Cucsat.G12648.T2 (mRNA) Cucumber (B10) v3

Overview
NameCucsat.G12648.T2
TypemRNA
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionFSH1 domain-containing protein
Locationctg1838: 1697952 .. 1698996 (-)
RNA-Seq ExpressionCucsat.G12648.T2
SyntenyCucsat.G12648.T2
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGTATGAGTATTGATTTTGTTGAAGTATAAAATAAACTGAGAAAAGAAATTGGAAATGGAAAACAGGGAAAGGTGTTGAAAGAGCATCCACCAATGAAAATGTTTGTGTCAATATCAGGAACAAAATTCAAAGATGAAAGCATATGTGAAGTTGCCTACAAACACAAGATAAAGGTTAAATCTGTTCACTTCATAGGTGAAAAGGATTGGTTGAAACTTCCTTCTCAACAACTTGCTTCGGTCTTCCATGAGCCTCTCATCATAAGGCATCCTCAAGGACATACAGTTCCTAGACTAGGTCATTTCTAGTCTACACACTATTCAACATATAAACATCTGTTCAACATGACGAGAGACTAATGTTTTTTTTTTTTTATAGATGAATCAGCTACTAAAGAGCTCAAATGTTGGGTTGATGCAATACTTTCTAGTCGTGATCATGATGGAGATGGAGAAGTGAAGGAGAATGTCATTGACAAAGTAGATGAGAGAATTGAAAATCAGATCCTGAAAGGGGAGAATAAATGTATCGATGTTGTTTAATTTTATAACATAAGTTGATAGGGAGAATGGATGAGTAAGCTTACAATCAATTGTGAGCTTTATCAAGGGGAGAAACAATATGTGGTGTCCATGCTTATTATGCTTCAAGCATACACATTTCAATTATGTCAAGTCTTGAATTTATGAATTGTGAAGTAATTTGATTGAACACCATAAACAGGGGCTTATATATATATATTAATTTCACCTAATCTATAGGGTTTGCAAATCCTATTTCAAAATAACTGTACAGGCAACGTAATGCTCAATCAATGAAGATCATATTAGATTTTTTTTCCCCCTCAAAAGTGATTCACAGCATCACTTTTCTTAGAACAAAATCTGTATAGAACTTACAGTTGTTGAAAGGCAATCAAGCACCACGGTTAGGGCAAACATTTGCGAAGTGTGACGGATCACCACAAACAAAACATCTTCTATCAGATATTGGCTCACCAGTTGCTGACACAAATGTTGCTGCAATCTGACCTTGACCCCGAT

Coding sequence (CDS)

ATGAAAATGTTTGTGTCAATATCAGGAACAAAATTCAAAGATGAAAGCATATGTGAAGTTGCCTACAAACACAAGATAAAGGTTAAATCTGTTCACTTCATAGGTGAAAAGGATTGGTTGAAACTTCCTTCTCAACAACTTGCTTCGGTCTTCCATGAGCCTCTCATCATAAGGCATCCTCAAGGACATACAGTTCCTAGACTAGATGAATCAGCTACTAAAGAGCTCAAATGTTGGGTTGATGCAATACTTTCTAGTCGTGATCATGATGGAGATGGAGAAGTGAAGGAGAATGTCATTGACAAAGTAGATGAGAGAATTGAAAATCAGATCCTGAAAGGGGAGAATAAATGTATCGATGTTGTTTAA

Protein sequence

MKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASVFHEPLIIRHPQGHTVPRLDESATKELKCWVDAILSSRDHDGDGEVKENVIDKVDERIENQILKGENKCIDVV
Homology
[Close]
BLAST of Cucsat.G12648.T2 vs. NCBI nr
Match: XP_004136600.1 (esterase OVCA2 [Cucumis sativus] >KGN59361.1 hypothetical protein Csa_001052 [Cucumis sativus])

HSP 1 Score: 250 bits (638), Expect = 8.77e-82
Identity = 122/122 (100.00%), Postives = 122/122 (100.00%), Query Frame = 0 

Query: 1   MKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASVFHEPLIIRHP 60
           MKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASVFHEPLIIRHP
Sbjct: 133 MKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASVFHEPLIIRHP 192

Query: 61  QGHTVPRLDESATKELKCWVDAILSSRDHDGDGEVKENVIDKVDERIENQILKGENKCID 120
           QGHTVPRLDESATKELKCWVDAILSSRDHDGDGEVKENVIDKVDERIENQILKGENKCID
Sbjct: 193 QGHTVPRLDESATKELKCWVDAILSSRDHDGDGEVKENVIDKVDERIENQILKGENKCID 252

Query: 121 VV 122
           VV
Sbjct: 253 VV 254

[Close]
BLAST of Cucsat.G12648.T2 vs. NCBI nr
Match: KAA0043679.1 (esterase C25G4.2-like [Cucumis melo var. makuwa] >TYK25452.1 esterase C25G4.2-like [Cucumis melo var. makuwa])

HSP 1 Score: 231 bits (589), Expect = 2.99e-76
Identity = 112/120 (93.33%), Postives = 116/120 (96.67%), Query Frame = 0 

Query: 3   MFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASVFHEPLIIRHPQG 62
           MFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASVFH+PLIIRHPQG
Sbjct: 1   MFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASVFHQPLIIRHPQG 60

Query: 63  HTVPRLDESATKELKCWVDAILSSRDHDGDGEVKENVIDKVDERIENQILKGENKCIDVV 122
           HTVPRLDESATKEL+CWVD ILSSR HDGD EVKE+VIDKVDERIENQI+K ENKCIDVV
Sbjct: 61  HTVPRLDESATKELQCWVDTILSSRGHDGDSEVKESVIDKVDERIENQIMKEENKCIDVV 120

[Close]
BLAST of Cucsat.G12648.T2 vs. NCBI nr
Match: XP_008443142.1 (PREDICTED: esterase C25G4.2-like [Cucumis melo])

HSP 1 Score: 233 bits (595), Expect = 2.83e-75
Identity = 113/122 (92.62%), Postives = 118/122 (96.72%), Query Frame = 0 

Query: 1   MKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASVFHEPLIIRHP 60
           +KMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASVFH+PLIIRHP
Sbjct: 131 IKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASVFHQPLIIRHP 190

Query: 61  QGHTVPRLDESATKELKCWVDAILSSRDHDGDGEVKENVIDKVDERIENQILKGENKCID 120
           QGHTVPRLDESATKEL+CWVD ILSSR HDGD EVKE+VIDKVDERIENQI+K ENKCID
Sbjct: 191 QGHTVPRLDESATKELQCWVDTILSSRGHDGDSEVKESVIDKVDERIENQIMKEENKCID 250

Query: 121 VV 122
           VV
Sbjct: 251 VV 252

[Close]
BLAST of Cucsat.G12648.T2 vs. NCBI nr
Match: XP_023539260.1 (family of serine hydrolases 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 197 bits (500), Expect = 6.41e-61
Identity = 93/111 (83.78%), Postives = 102/111 (91.89%), Query Frame = 0 

Query: 1   MKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASVFHEPLIIRHP 60
           MKMFVSISGTKFKDESICEVAYKHKIKVKSVH IGEKDWLKLPS++LAS FHEPLIIRHP
Sbjct: 131 MKMFVSISGTKFKDESICEVAYKHKIKVKSVHLIGEKDWLKLPSEELASAFHEPLIIRHP 190

Query: 61  QGHTVPRLDESATKELKCWVDAILSSRDHDGDGEVKENVIDKVDERIENQI 111
           QGHTVPRLDE AT+EL+CWVDAI+SS DH GDGEVKEN+  KV + IEN++
Sbjct: 191 QGHTVPRLDEVATEELRCWVDAIVSSHDHVGDGEVKENITQKVADNIENEL 241

[Close]
BLAST of Cucsat.G12648.T2 vs. NCBI nr
Match: KAG6596208.1 (Esterase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 195 bits (496), Expect = 2.59e-60
Identity = 92/111 (82.88%), Postives = 101/111 (90.99%), Query Frame = 0 

Query: 1   MKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASVFHEPLIIRHP 60
           MKMFVSISGTKFKDESICEVAYKHKIKVKSVH IGEKDWLKLPS++LAS FHEPLIIRHP
Sbjct: 131 MKMFVSISGTKFKDESICEVAYKHKIKVKSVHLIGEKDWLKLPSEELASAFHEPLIIRHP 190

Query: 61  QGHTVPRLDESATKELKCWVDAILSSRDHDGDGEVKENVIDKVDERIENQI 111
           QGHTVPRLDE ATK+L+CWVDAI+SS DH GDGE KEN+  KV + IEN++
Sbjct: 191 QGHTVPRLDEVATKKLRCWVDAIVSSHDHVGDGEAKENITQKVADNIENEL 241

[Close]
BLAST of Cucsat.G12648.T2 vs. ExPASy TrEMBL
Match: A0A0A0LH35 (FSH1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G813300 PE=4 SV=1)

HSP 1 Score: 250 bits (638), Expect = 4.24e-82
Identity = 122/122 (100.00%), Postives = 122/122 (100.00%), Query Frame = 0 

Query: 1   MKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASVFHEPLIIRHP 60
           MKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASVFHEPLIIRHP
Sbjct: 133 MKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASVFHEPLIIRHP 192

Query: 61  QGHTVPRLDESATKELKCWVDAILSSRDHDGDGEVKENVIDKVDERIENQILKGENKCID 120
           QGHTVPRLDESATKELKCWVDAILSSRDHDGDGEVKENVIDKVDERIENQILKGENKCID
Sbjct: 193 QGHTVPRLDESATKELKCWVDAILSSRDHDGDGEVKENVIDKVDERIENQILKGENKCID 252

Query: 121 VV 122
           VV
Sbjct: 253 VV 254

[Close]
BLAST of Cucsat.G12648.T2 vs. ExPASy TrEMBL
Match: A0A5A7TR68 (Esterase C25G4.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005980 PE=4 SV=1)

HSP 1 Score: 231 bits (589), Expect = 1.45e-76
Identity = 112/120 (93.33%), Postives = 116/120 (96.67%), Query Frame = 0 

Query: 3   MFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASVFHEPLIIRHPQG 62
           MFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASVFH+PLIIRHPQG
Sbjct: 1   MFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASVFHQPLIIRHPQG 60

Query: 63  HTVPRLDESATKELKCWVDAILSSRDHDGDGEVKENVIDKVDERIENQILKGENKCIDVV 122
           HTVPRLDESATKEL+CWVD ILSSR HDGD EVKE+VIDKVDERIENQI+K ENKCIDVV
Sbjct: 61  HTVPRLDESATKELQCWVDTILSSRGHDGDSEVKESVIDKVDERIENQIMKEENKCIDVV 120

[Close]
BLAST of Cucsat.G12648.T2 vs. ExPASy TrEMBL
Match: A0A1S3B6W5 (esterase C25G4.2-like OS=Cucumis melo OX=3656 GN=LOC103486820 PE=4 SV=1)

HSP 1 Score: 233 bits (595), Expect = 1.37e-75
Identity = 113/122 (92.62%), Postives = 118/122 (96.72%), Query Frame = 0 

Query: 1   MKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASVFHEPLIIRHP 60
           +KMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASVFH+PLIIRHP
Sbjct: 131 IKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASVFHQPLIIRHP 190

Query: 61  QGHTVPRLDESATKELKCWVDAILSSRDHDGDGEVKENVIDKVDERIENQILKGENKCID 120
           QGHTVPRLDESATKEL+CWVD ILSSR HDGD EVKE+VIDKVDERIENQI+K ENKCID
Sbjct: 191 QGHTVPRLDESATKELQCWVDTILSSRGHDGDSEVKESVIDKVDERIENQIMKEENKCID 250

Query: 121 VV 122
           VV
Sbjct: 251 VV 252

[Close]
BLAST of Cucsat.G12648.T2 vs. ExPASy TrEMBL
Match: A0A6J1I8F2 (uncharacterized protein LOC111470131 OS=Cucurbita maxima OX=3661 GN=LOC111470131 PE=4 SV=1)

HSP 1 Score: 185 bits (470), Expect = 5.84e-58
Identity = 87/111 (78.38%), Postives = 98/111 (88.29%), Query Frame = 0 

Query: 1   MKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASVFHEPLIIRHP 60
           MKMF+SISGT FKDESICEVAYKHKIKVKSVH IGEKDWLKLPS++LAS FHEPLIIRHP
Sbjct: 35  MKMFLSISGTNFKDESICEVAYKHKIKVKSVHLIGEKDWLKLPSEELASAFHEPLIIRHP 94

Query: 61  QGHTVPRLDESATKELKCWVDAILSSRDHDGDGEVKENVIDKVDERIENQI 111
           QGHTVPRLDE AT+EL+CWVDAI+SS +  GD E KEN+  K+ E IEN++
Sbjct: 95  QGHTVPRLDEVATEELRCWVDAIVSSHERVGDAEAKENITQKMAENIENEL 145

[Close]
BLAST of Cucsat.G12648.T2 vs. ExPASy TrEMBL
Match: A0A6J1DLE8 (dihydrofolate reductase-like OS=Momordica charantia OX=3673 GN=LOC111021534 PE=4 SV=1)

HSP 1 Score: 179 bits (454), Expect = 3.27e-54
Identity = 88/115 (76.52%), Postives = 101/115 (87.83%), Query Frame = 0 

Query: 1   MKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASVFHEPLIIRHP 60
           +K+ VSISG+KF+DE ICEVAYKHKI+ KSVH IGEKDWLKLPSQ+LAS FH+PLIIRHP
Sbjct: 130 IKLLVSISGSKFRDEKICEVAYKHKIEAKSVHLIGEKDWLKLPSQELASAFHDPLIIRHP 189

Query: 61  QGHTVPRLDESATKELKCWVDAILSSRDHD-GDGEVKEN-VIDKVDERIENQILK 113
           QGHTVPRLDE+ATKEL+CWVDA+LS  DH   D EVKEN VIDK+DE+IEN + K
Sbjct: 190 QGHTVPRLDEAATKELQCWVDAVLSGHDHVIEDEEVKENYVIDKMDEKIENGLSK 244

The following BLAST results are available for this feature:
BLAST of Cucsat.G12648.T2 vs.
Match NameE-valueIdentityDescription
BLAST of Cucsat.G12648.T2 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004136600.18.77e-82100.00esterase OVCA2 [Cucumis sativus] >KGN59361.1 hypothetical protein Csa_001052 [Cu... [more]
KAA0043679.12.99e-7693.33esterase C25G4.2-like [Cucumis melo var. makuwa] >TYK25452.1 esterase C25G4.2-li... [more]
XP_008443142.12.83e-7592.62PREDICTED: esterase C25G4.2-like [Cucumis melo][more]
XP_023539260.16.41e-6183.78family of serine hydrolases 2-like [Cucurbita pepo subsp. pepo][more]
KAG6596208.12.59e-6082.88Esterase-like protein, partial [Cucurbita argyrosperma subsp. sororia][more]
BLAST of Cucsat.G12648.T2 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LH354.24e-82100.00FSH1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G813300 PE=4 S... [more]
A0A5A7TR681.45e-7693.33Esterase C25G4.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35... [more]
A0A1S3B6W51.37e-7592.62esterase C25G4.2-like OS=Cucumis melo OX=3656 GN=LOC103486820 PE=4 SV=1[more]
A0A6J1I8F25.84e-5878.38uncharacterized protein LOC111470131 OS=Cucurbita maxima OX=3661 GN=LOC111470131... [more]
A0A6J1DLE83.27e-5476.52dihydrofolate reductase-like OS=Momordica charantia OX=3673 GN=LOC111021534 PE=4... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005645Serine hydrolase FSHPFAMPF03959FSH1coord: 1..71
e-value: 5.5E-12
score: 45.8
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 1..88
e-value: 5.4E-11
score: 44.4
NoneNo IPR availablePANTHERPTHR48070:SF10OS07G0687100 PROTEINcoord: 1..97
NoneNo IPR availablePANTHERPTHR48070ESTERASE OVCA2coord: 1..97

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsat.G12648Cucsat.G12648gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G12648.T2.E1Cucsat.G12648.T2.E1exon
Cucsat.G12648.T2.E2Cucsat.G12648.T2.E2exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsat.G12648.T2.C2Cucsat.G12648.T2.C2CDS
Cucsat.G12648.T2.C1Cucsat.G12648.T2.C1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsat.G12648.T2Cucsat.G12648.T2-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0016787 hydrolase activity