Cucsat.G12648 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G12648
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	FSH1 domain-containing protein
Location	ctg1838: 1697941 .. 1700859 (-)
RNA-Seq Expression	Cucsat.G12648
Synteny	Cucsat.G12648

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AATGAAGTTGGGGGGAAAAGAAAGGGTTGTGGTGTATGTGGAAGTGAAATGAAATGGAGATGAGTAGCAAAAGAGATGATAAAAAAAGATTGAAGATATTGTGCCTTCATGGATTTAGAACAAATGGAAGTTTTCTTAGAAAGCAAATTAGCAAATGGGATCCTTCCATTTTTGCTCATTTTGATATGGTATGTTATGTTATATGTATGTATACATCTTCCAACGCAGTTTTTTTGTTTTAAATTTTGATAAACTGTTGTACGTAATTAATATCACTATCTCAATACGATAGGGGTGTTATTGGATTAATTTACGGGTCAAGTAATTTCTTTGTAGAGAAGGCTTTCATATTTCTCCATATTAACAACTTACATTGTGGGTTAAATTCCATTGTAACGGTTTGAAAGAAAGGAAAAAAAAAAAAAACTTTTGAAAAGCTTTTTTTTGTGATGAAAACGTTTCAAAACTTTAAAGATTTAGGGTTTAGTAGGAAACCTTTTTTTTTTTTTTGAGACGTAGTATCGTTCTAATTATGTTTGAGACTTTTTTTTATTCTTTATATCAAACTATGATAATACGAACTTATGAGCATTTGAGTTTAGTGTTCATTTTAATTATTTATTTATTTATTTATATGTTCTTTATATATTTAATTTTTGTAGTTATGTCAATTTCTTAAAATCGTACACTTGCACTGAATTCACACTTTTTTTAGTTAATTCGAGAAACAGTCAATTTTATATTCATTCAATTTCAACCTTACGATGAAAATGAAAATTTTGACCAAAACACCCTTTACTTGACCTAATTTATAACATATGTTAGTGACTAAATAATTTGTAGTTCAATTCTTCGACGAGTAAATTTTACGATAAAATATAAATTCAAATGCATGATATATATAATAAGAGTAATAGTAATAATTTGTACATTTAAACAGGAATTTCCAGATGGTATATTTCCAGCAGGAGGAAAATCAGAGATAGAAGGATATTTTCCACCTCCTTATTTTGAATGGTTTCAATTTAATGAGGTACAATTTAAAATCAAATATTTTTTTCTAATATCTCAATATATGTATGCATGTGTGTGTATATATATATCAAAGAAAGTGATATATATATCTTGTTTTAATTTGAACATGGCAGGATTTCACAGAATACAAGAACTTGGAAGAATGCATAGATTATGTATGCAACTACATCACTACAAATGGCCCTTTTCATGGCTTATTGGGTTTTTCTCAAGTATGTTTCTTTTATTTTCTCTTTTAGATCTTGTTTTAAAAAGCATACATTATTGTATTTTCAAATTCACAAAATTTAAATTTTAAATTGAAACAAAATGTATTTTCAAATATAATGAAAAAAAATAATTTATTTTTAAAAATTGCTTTATTGTTGATAAAATAGATAACTATGTTTAAATAACCTTCTAAATAATTTCAAAGGTATATTTTTAAGGGTTTATTATGACATTGTTATATGGTAGAGTAATTAAAATGAAAATTATTTTAAATACCTTGATTTTACTGTATTTAAAAATAAGCCTAATTAAAATTTTACTCATTATTCAGGGAGCAGTATTGTCAGCACTCTTATTGGGTTACCAAGCACAGGTATACATTAATTAATCGAAAATATATCTAAAATAAGTGAAATAACCATTTTGATTTCAAATGAGTGAAATAAAAACATTGCAAAAAGTATAGTTATATTGATTAAGTTAGTGAATGATTGCAATAAATTATATCTAAGTCAAAGAGGATTAGTCATGACATCCATTTACATTTGATTTGAACAAAAAAACAATCTGGAAGAGTATGATTTTGAAGCAAATGTTAATATTGAAACTTCTGAATGTTTGTATGAGTATTGATTTTGTTGAAGTATAAAATAAACTGAGAAAAGAAATTGGAAATGGAAAACAGGGAAAGGTGTTGAAAGAGCATCCACCAATGAAAATGTTTGTGTCAATATCAGGAACAAAATTCAAAGATGAAAGCATATGTGAAGTTGCCTACAAACACAAGATAAAGGTTAAATCTGTTCACTTCATAGGTGAAAAGGATTGGTTGAAACTTCCTTCTCAACAACTTGCTTCGGTCTTCCATGAGCCTCTCATCATAAGGCATCCTCAAGGACATACAGTTCCTAGACTAGGTCATTTCTAGTCTACACACTATTCAACATATAAACATCTGTTCAACATGACGAGAGACTAATGTTTTTTTTTTTTTATAGATGAATCAGCTACTAAAGAGCTCAAATGTTGGGTTGATGCAATACTTTCTAGTCGTGATCATGATGGAGATGGAGAAGTGAAGGAGAATGTCATTGACAAAGTAGATGAGAGAATTGAAAATCAGATCCTGAAAGGGGAGAATAAATGTATCGATGTTGTTTAATTTTATAACATAAGTTGATAGGGAGAATGGATGAGTAAGCTTACAATCAATTGTGAGCTTTATCAAGGGGAGAAACAATATGTGGTGTCCATGCTTATTATGCTTCAAGCATACACATTTCAATTATGTCAAGTCTTGAATTTATGAATTGTGAAGTAATTTGATTGAACACCATAAACAGGGGCTTATATATATATATTAATTTCACCTAATCTATAGGGTTTGCAAATCCTATTTCAAAATAACTGTACAGGCAACGTAATGCTCAATCAATGAAGATCATATTAGATTTTTTTTCCCCCTCAAAAGTGATTCACAGCATCACTTTTCTTAGAACAAAATCTGTATAGAACTTACAGTTGTTGAAAGGCAATCAAGCACCACGGTTAGGGCAAACATTTGCGAAGTGTGACGGATCACCACAAACAAAACATCTTCTATCAGATATTGGCTCACCAGTTGCTGACACAAATGTTGCTGCAATCTGACCTTGACCCCGATCCCGATCCCGA

Coding sequence (CDS)

ATGGAGATGAGTAGCAAAAGAGATGATAAAAAAAGATTGAAGATATTGTGCCTTCATGGATTTAGAACAAATGGAAGTTTTCTTAGAAAGCAAATTAGCAAATGGGATCCTTCCATTTTTGCTCATTTTGATATGGAATTTCCAGATGGTATATTTCCAGCAGGAGGAAAATCAGAGATAGAAGGATATTTTCCACCTCCTTATTTTGAATGGTTTCAATTTAATGAGGATTTCACAGAATACAAGAACTTGGAAGAATGCATAGATTATGTATGCAACTACATCACTACAAATGGCCCTTTTCATGGCTTATTGGGTTTTTCTCAAGGAGCAGTATTGTCAGCACTCTTATTGGGTTACCAAGCACAGGGAAAGGTGTTGAAAGAGCATCCACCAATGAAAATGTTTGTGTCAATATCAGGAACAAAATTCAAAGATGAAAGCATATGTGAAGTTGCCTACAAACACAAGATAAAGGTTAAATCTGTTCACTTCATAGGTGAAAAGGATTGGTTGAAACTTCCTTCTCAACAACTTGCTTCGGTCTTCCATGAGCCTCTCATCATAAGGCATCCTCAAGGACATACAGTTCCTAGACTAGATGAATCAGCTACTAAAGAGCTCAAATGTTGGGTTGATGCAATACTTTCTAGTCGTGATCATGATGGAGATGGAGAAGTGAAGGAGAATGTCATTGACAAAGTAGATGAGAGAATTGAAAATCAGATCCTGAAAGGGGAGAATAAATGTATCGATGTTGTTTAA

Protein sequence

MEMSSKRDDKKRLKILCLHGFRTNGSFLRKQISKWDPSIFAHFDMEFPDGIFPAGGKSEIEGYFPPPYFEWFQFNEDFTEYKNLEECIDYVCNYITTNGPFHGLLGFSQGAVLSALLLGYQAQGKVLKEHPPMKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASVFHEPLIIRHPQGHTVPRLDESATKELKCWVDAILSSRDHDGDGEVKENVIDKVDERIENQILKGENKCIDVV

Homology

[Close]

BLAST of Cucsat.G12648 vs. ExPASy Swiss-Prot
Match: P36591 (Dihydrofolate reductase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dfr1 PE=2 SV=2)

HSP 1 Score: 84.0 bits (206), Expect = 2.8e-15
Identity = 56/206 (27.18%), Postives = 92/206 (44.66%), Query Frame = 0


Query: 11  KRLKILCLHGFRTNGSFLRKQISKWDPSIFAHFDMEFPDGIFPAGGKSE----------- 70
           K LK+LCLHG+  +G    K++      +  + ++ FP G   A  +++           
Sbjct: 3   KPLKVLCLHGWIQSGPVFSKKMGSVQKYLSKYAELHFPTGPVVADEEADPNDEEEKKRLA 62

Query: 71  -IEGYFPPPYFEWFQFNEDFTEYKNLEECIDYVCNYITTNGPFHGLLGFSQGAVLSALLL 130
            + G      F WF+  +    Y + +E ++ +  Y+   GPF GL+GFSQGA + A+L 
Sbjct: 63  ALGGEQNGGKFGWFEVEDFKNTYGSWDESLECINQYMQEKGPFDGLIGFSQGAGIGAMLA 122

Query: 131 GYQAQGK---VLKEHPPMKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWL--K 190
                G+      +HPP K  V + G + +     +  Y  K+   S+H  G  D L   
Sbjct: 123 QMLQPGQPPNPYVQHPPFKFVVFVGGFRAEKPEF-DHFYNPKLTTPSLHIAGTSDTLVPL 182

Query: 191 LPSQQLASVFHEPLIIRHPQGHTVPR 200
             S+QL        ++ HP  H VP+
Sbjct: 183 ARSKQLVERCENAHVLLHPGQHIVPQ 207

[Close]

BLAST of Cucsat.G12648 vs. ExPASy Swiss-Prot
Match: Q99369 (Family of serine hydrolases 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=FSH3 PE=2 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.8e-14
Identity = 73/268 (27.24%), Postives = 120/268 (44.78%), Query Frame = 0


Query: 11  KRLKILCLHGF---------RTNGSFLRKQISKWDPSIF-----------AHFDMEFPDG 70
           ++ K+L LHGF         +T G  LRK + K    ++           A F  E   G
Sbjct: 3   EKKKVLMLHGFVQSDKIFSAKTGG--LRKNLKKLGYDLYYPCAPHSIDKKALFQSESEKG 62

Query: 71  ------IFPAGGKSEIEGYFPPPYFEWFQFNEDFTEYKNLEECIDYVCNYITTNGPFHGL 130
                    +    E+ G+F       F+  E F  ++  ++  +Y+ NY+  NGPF G+
Sbjct: 63  RDAAKEFNTSATSDEVYGWF-------FRNPESFNSFQIDQKVFNYLRNYVLENGPFDGV 122

Query: 131 LGFSQGAVLSALLLG--YQAQGKVLKEHPPMKMFVSISGTKFKDESICEVAYKHKIKVKS 190
           +GFSQGA L   L+    +      ++ P +K F+S SG K +D+S  +  Y   I+V S
Sbjct: 123 IGFSQGAGLGGYLVTDFNRILNLTDEQQPALKFFISFSGFKLEDQSY-QKEYHRIIQVPS 182

Query: 191 VHFIGEKDWLKLPSQQLASVFHEP----LIIRHPQGHTVPRLDESATKELKCWVDAILS- 245
           +H  GE D +   S+ +A     P     ++ HP  H VP   +    ++  W+  I S 
Sbjct: 183 LHVRGELDEVVAESRIMALYESWPDNKRTLLVHPGAHFVPN-SKPFVSQVCNWIQGITSK 242

[Close]

BLAST of Cucsat.G12648 vs. ExPASy Swiss-Prot
Match: Q7QBJ0 (Esterase AGAP003155 OS=Anopheles gambiae OX=7165 GN=AGAP003155 PE=3 SV=3)

HSP 1 Score: 80.1 bits (196), Expect = 4.0e-14
Identity = 64/245 (26.12%), Postives = 110/245 (44.90%), Query Frame = 0


Query: 10  KKRLKILCLHGFRTNGSFLRKQISKWDPSIFAHFDMEFPDGIFPAGGKSEIEGYFPPPYF 69
           +++LK+L LHG+R N    + ++  +   +  + +  F     PA     + G   P   
Sbjct: 9   EEKLKVLALHGYRQNADSFKSKLGSFRKMLNKYVEFVFVSAPHPAAPLEAVGGEPDPNQR 68

Query: 70  EWFQFNEDFTEYK---------NLEECIDYVCNYITTNGPFHGLLGFSQGAVLSALLLGY 129
            W+ FN+D   +K           +E +  V       G  HGLLGFSQGA    LL   
Sbjct: 69  SWW-FNKDDRTFKGTNQGGPAYGFDESLRLVERTWQAEG-CHGLLGFSQGACFVGLLCDL 128

Query: 130 QAQGKVLKEHPPMKMFVSISGTKFKDESICEV-AYKHKIKVKSVHFIGEKDWL--KLPSQ 189
            A+G        MK   ++  + F+  S+  +  Y++K+++ S+H  GE D +  K  S+
Sbjct: 129 SARGMT-----TMKPQFAVVASGFRSGSLVHLNYYENKVQIPSLHIFGETDEIITKDMSE 188

Query: 190 QLASVFHEPLIIRHPQGHTVPRLDESATKELKCWVDAILSSRDHDGDGEVKENVIDKVDE 243
            LA  F +P ++ HP GH  P   +++ KE   +VD          + +  +N  ++   
Sbjct: 189 ALAETFLDPEVVTHPGGHYFPA--QASLKE--TYVDFFRDQLQQHLEAKELQNATEENSF 242

[Close]

BLAST of Cucsat.G12648 vs. ExPASy Swiss-Prot
Match: Q18169 (Esterase C25G4.2 OS=Caenorhabditis elegans OX=6239 GN=C25G4.2 PE=3 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.5e-13
Identity = 61/209 (29.19%), Postives = 99/209 (47.37%), Query Frame = 0


Query: 12  RLKILCLHGFRTNGSFLRKQISKWDPSIFAHFDMEFPDGIFPAGGKSEIEGYFPPPYFEW 71
           +L+ILCLHG+R      R++       + +  + EF +G+        ++ +       W
Sbjct: 6   KLRILCLHGYRQCDQSFRQKTGSTRKLVKSLAEFEFVNGVHSVA----VDEHVDSSRAWW 65

Query: 72  FQFNE-------DFTEYK-NLEECIDYVCNYITTNGPFHGLLGFSQGAVLSALLLGYQAQ 131
           F  NE       + TE     EE +  V  +I  NGPF GLLGFSQGA +  LL+     
Sbjct: 66  FSNNEAMSFSSRESTEVAVGFEESVAAVVKFIEENGPFDGLLGFSQGASMVHLLIAKAQL 125

Query: 132 GKVLKEHPPMKMFVSISG---TKFKDESICEVAYKHKIKVKSVHFIGEKDWL--KLPSQQ 191
           G++  + P ++  +  SG      K +S+  +  K   +  S+H  G+ D +  +  S++
Sbjct: 126 GEI--KLPGIRFAIFFSGFLSLSSKHDSLTLLRIK---EFPSMHVFGDADEIVARPKSEK 185

Query: 192 LASVFH-EPLIIRHPQGHTVPRLDESATK 207
           +A +F  EPL I H  GH VP + +   K
Sbjct: 186 MADMFDVEPLRIAHDGGHVVPSMSKHKEK 205

[Close]

BLAST of Cucsat.G12648 vs. ExPASy Swiss-Prot
Match: A4II73 (Esterase OVCA2 OS=Xenopus tropicalis OX=8364 GN=ovca2 PE=2 SV=2)

HSP 1 Score: 77.4 bits (189), Expect = 2.6e-13
Identity = 65/206 (31.55%), Postives = 91/206 (44.17%), Query Frame = 0


Query: 10  KKRLKILCLHGFRTN--------GSFLRKQISKWDPSIF-AHFDMEFPDGIFPAGGKSEI 69
           K+ L++L LHG+R N        G+  ++   + D   F A   +  PD    AG    +
Sbjct: 12  KRVLRVLALHGYRQNERSFWERTGALRKRLRGRADLITFSAPLLVPDPDAEPGAGDPDSL 71

Query: 70  EG-----YFPPPYFEWFQFNEDFTEYKNLEECIDYVCNYITTNGPFHGLLGFSQGAVLSA 129
           +      +F  P    F   E+      LE  +D V    +  GPF G+LGFSQGA L A
Sbjct: 72  QDESRGWWFSNPEQNSFDAMEESKTCSGLEAPLDTVAKAFSELGPFDGILGFSQGAALVA 131

Query: 130 LLLGYQAQGKVLKEHPPMKMFVSISGTKFKDESICEVA-YKHKIKVKSVHFIGEKDWL-- 189
           ++   + QG      P      +I    FK  S      Y+  I V S+H IGE D +  
Sbjct: 132 IICALKQQG-----DPRFHFDFAILVAGFKSLSTDHAKHYQQPITVPSLHVIGETDRVIS 191

Query: 190 KLPSQQLASVFHEPLIIRHPQGHTVP 199
              SQ+L S F  P+I+ H  GH VP
Sbjct: 192 AAMSQELVSHFENPVILMHSGGHYVP 212

[Close]

BLAST of Cucsat.G12648 vs. NCBI nr
Match: XP_004136600.1 (esterase OVCA2 [Cucumis sativus] >KGN59361.1 hypothetical protein Csa_001052 [Cucumis sativus])

HSP 1 Score: 528 bits (1359), Expect = 2.80e-189
Identity = 254/254 (100.00%), Postives = 254/254 (100.00%), Query Frame = 0


Query: 1   MEMSSKRDDKKRLKILCLHGFRTNGSFLRKQISKWDPSIFAHFDMEFPDGIFPAGGKSEI 60
           MEMSSKRDDKKRLKILCLHGFRTNGSFLRKQISKWDPSIFAHFDMEFPDGIFPAGGKSEI
Sbjct: 1   MEMSSKRDDKKRLKILCLHGFRTNGSFLRKQISKWDPSIFAHFDMEFPDGIFPAGGKSEI 60

Query: 61  EGYFPPPYFEWFQFNEDFTEYKNLEECIDYVCNYITTNGPFHGLLGFSQGAVLSALLLGY 120
           EGYFPPPYFEWFQFNEDFTEYKNLEECIDYVCNYITTNGPFHGLLGFSQGAVLSALLLGY
Sbjct: 61  EGYFPPPYFEWFQFNEDFTEYKNLEECIDYVCNYITTNGPFHGLLGFSQGAVLSALLLGY 120

Query: 121 QAQGKVLKEHPPMKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLA 180
           QAQGKVLKEHPPMKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLA
Sbjct: 121 QAQGKVLKEHPPMKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLA 180

Query: 181 SVFHEPLIIRHPQGHTVPRLDESATKELKCWVDAILSSRDHDGDGEVKENVIDKVDERIE 240
           SVFHEPLIIRHPQGHTVPRLDESATKELKCWVDAILSSRDHDGDGEVKENVIDKVDERIE
Sbjct: 181 SVFHEPLIIRHPQGHTVPRLDESATKELKCWVDAILSSRDHDGDGEVKENVIDKVDERIE 240

Query: 241 NQILKGENKCIDVV 254
           NQILKGENKCIDVV
Sbjct: 241 NQILKGENKCIDVV 254

[Close]

BLAST of Cucsat.G12648 vs. NCBI nr
Match: XP_008443142.1 (PREDICTED: esterase C25G4.2-like [Cucumis melo])

HSP 1 Score: 487 bits (1253), Expect = 3.78e-173
Identity = 233/252 (92.46%), Postives = 243/252 (96.43%), Query Frame = 0


Query: 3   MSSKRDDKKRLKILCLHGFRTNGSFLRKQISKWDPSIFAHFDMEFPDGIFPAGGKSEIEG 62
           M S + D++RLKILCLHGFRTNGSFLRKQ+SKWDPSIFA F MEFPDGIFPAGGKSEIEG
Sbjct: 1   MESGKRDEERLKILCLHGFRTNGSFLRKQLSKWDPSIFALFHMEFPDGIFPAGGKSEIEG 60

Query: 63  YFPPPYFEWFQFNEDFTEYKNLEECIDYVCNYITTNGPFHGLLGFSQGAVLSALLLGYQA 122
           YFPPPYFEWFQFNEDFTEYKNLEECIDYVCNYITTNGPFHGLLGFSQGAVLSALLLGYQA
Sbjct: 61  YFPPPYFEWFQFNEDFTEYKNLEECIDYVCNYITTNGPFHGLLGFSQGAVLSALLLGYQA 120

Query: 123 QGKVLKEHPPMKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASV 182
           QGKVL+EHPP+KMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASV
Sbjct: 121 QGKVLREHPPIKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASV 180

Query: 183 FHEPLIIRHPQGHTVPRLDESATKELKCWVDAILSSRDHDGDGEVKENVIDKVDERIENQ 242
           FH+PLIIRHPQGHTVPRLDESATKEL+CWVD ILSSR HDGD EVKE+VIDKVDERIENQ
Sbjct: 181 FHQPLIIRHPQGHTVPRLDESATKELQCWVDTILSSRGHDGDSEVKESVIDKVDERIENQ 240

Query: 243 ILKGENKCIDVV 254
           I+K ENKCIDVV
Sbjct: 241 IMKEENKCIDVV 252

[Close]

BLAST of Cucsat.G12648 vs. NCBI nr
Match: KAG6596208.1 (Esterase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 434 bits (1115), Expect = 3.40e-152
Identity = 206/243 (84.77%), Postives = 223/243 (91.77%), Query Frame = 0


Query: 1   MEMSSKRDDKKRLKILCLHGFRTNGSFLRKQISKWDPSIFAHFDMEFPDGIFPAGGKSEI 60
           ME   KR +  RLKILCLHGFRT+GSFLRKQ++KWDPSIFAHFDMEFPD IFP+GGKSEI
Sbjct: 1   MESGGKRAE--RLKILCLHGFRTSGSFLRKQLTKWDPSIFAHFDMEFPDAIFPSGGKSEI 60

Query: 61  EGYFPPPYFEWFQFNEDFTEYKNLEECIDYVCNYITTNGPFHGLLGFSQGAVLSALLLGY 120
           EGYFPPPYFEWFQFNEDFTEY NLE+CIDY+ NYIT+NGPFHGLLGFSQGAVLSALLLGY
Sbjct: 61  EGYFPPPYFEWFQFNEDFTEYTNLEQCIDYLNNYITSNGPFHGLLGFSQGAVLSALLLGY 120

Query: 121 QAQGKVLKEHPPMKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLA 180
           QAQGKVLKEHP MKMFVSISGTKFKDESICEVAYKHKIKVKSVH IGEKDWLKLPS++LA
Sbjct: 121 QAQGKVLKEHPAMKMFVSISGTKFKDESICEVAYKHKIKVKSVHLIGEKDWLKLPSEELA 180

Query: 181 SVFHEPLIIRHPQGHTVPRLDESATKELKCWVDAILSSRDHDGDGEVKENVIDKVDERIE 240
           S FHEPLIIRHPQGHTVPRLDE ATK+L+CWVDAI+SS DH GDGE KEN+  KV + IE
Sbjct: 181 SAFHEPLIIRHPQGHTVPRLDEVATKKLRCWVDAIVSSHDHVGDGEAKENITQKVADNIE 240

Query: 241 NQI 243
           N++
Sbjct: 241 NEL 241

[Close]

BLAST of Cucsat.G12648 vs. NCBI nr
Match: XP_023539260.1 (family of serine hydrolases 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 432 bits (1112), Expect = 9.74e-152
Identity = 208/243 (85.60%), Postives = 222/243 (91.36%), Query Frame = 0


Query: 1   MEMSSKRDDKKRLKILCLHGFRTNGSFLRKQISKWDPSIFAHFDMEFPDGIFPAGGKSEI 60
           ME   KR   KRLKILCLHGFRT+GSFLRKQ++KWDPSIFAHFDMEFPD IFPAGGKSEI
Sbjct: 1   MESGGKRA--KRLKILCLHGFRTSGSFLRKQLTKWDPSIFAHFDMEFPDAIFPAGGKSEI 60

Query: 61  EGYFPPPYFEWFQFNEDFTEYKNLEECIDYVCNYITTNGPFHGLLGFSQGAVLSALLLGY 120
           EGYFPPPYFEWFQFNEDFTEY NLE+ IDY+ NYIT NGPFHGLLGFSQGAVLSALLLGY
Sbjct: 61  EGYFPPPYFEWFQFNEDFTEYTNLEQSIDYLNNYITCNGPFHGLLGFSQGAVLSALLLGY 120

Query: 121 QAQGKVLKEHPPMKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLA 180
           QAQ KVLKEHPPMKMFVSISGTKFKDESICEVAYKHKIKVKSVH IGEKDWLKLPS++LA
Sbjct: 121 QAQEKVLKEHPPMKMFVSISGTKFKDESICEVAYKHKIKVKSVHLIGEKDWLKLPSEELA 180

Query: 181 SVFHEPLIIRHPQGHTVPRLDESATKELKCWVDAILSSRDHDGDGEVKENVIDKVDERIE 240
           S FHEPLIIRHPQGHTVPRLDE AT+EL+CWVDAI+SS DH GDGEVKEN+  KV + IE
Sbjct: 181 SAFHEPLIIRHPQGHTVPRLDEVATEELRCWVDAIVSSHDHVGDGEVKENITQKVADNIE 240

Query: 241 NQI 243
           N++
Sbjct: 241 NEL 241

[Close]

BLAST of Cucsat.G12648 vs. NCBI nr
Match: XP_022154224.1 (dihydrofolate reductase-like [Momordica charantia])

HSP 1 Score: 412 bits (1058), Expect = 1.90e-143
Identity = 196/244 (80.33%), Postives = 222/244 (90.98%), Query Frame = 0


Query: 4   SSKRDDKKRLKILCLHGFRTNGSFLRKQISKWDPSIFAHFDMEFPDGIFPAGGKSEIEGY 63
           S KR++K  +KILCLHGFRT+GSFLRKQ+SKWDPSIFAHFD++FPDG+ PAGGKS+IEGY
Sbjct: 3   SGKRENK--MKILCLHGFRTSGSFLRKQLSKWDPSIFAHFDLDFPDGMCPAGGKSDIEGY 62

Query: 64  FPPPYFEWFQFNEDFTEYKNLEECIDYVCNYITTNGPFHGLLGFSQGAVLSALLLGYQAQ 123
           FPPPYFEWFQFN++FTEY NLE+CI Y+CNYITTNGPF GLLGFSQGA LSALLLGYQAQ
Sbjct: 63  FPPPYFEWFQFNQEFTEYTNLEQCIGYLCNYITTNGPFDGLLGFSQGATLSALLLGYQAQ 122

Query: 124 GKVLKEHPPMKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASVF 183
           GKVLKEHPP+K+ VSISG+KF+DE ICEVAYKHKI+ KSVH IGEKDWLKLPSQ+LAS F
Sbjct: 123 GKVLKEHPPIKLLVSISGSKFRDEKICEVAYKHKIEAKSVHLIGEKDWLKLPSQELASAF 182

Query: 184 HEPLIIRHPQGHTVPRLDESATKELKCWVDAILSSRDHD-GDGEVKEN-VIDKVDERIEN 243
           H+PLIIRHPQGHTVPRLDE+ATKEL+CWVDA+LS  DH   D EVKEN VIDK+DE+IEN
Sbjct: 183 HDPLIIRHPQGHTVPRLDEAATKELQCWVDAVLSGHDHVIEDEEVKENYVIDKMDEKIEN 242

Query: 244 QILK 245
            + K
Sbjct: 243 GLSK 244

[Close]

BLAST of Cucsat.G12648 vs. ExPASy TrEMBL
Match: A0A0A0LH35 (FSH1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G813300 PE=4 SV=1)

HSP 1 Score: 528 bits (1359), Expect = 1.36e-189
Identity = 254/254 (100.00%), Postives = 254/254 (100.00%), Query Frame = 0


Query: 1   MEMSSKRDDKKRLKILCLHGFRTNGSFLRKQISKWDPSIFAHFDMEFPDGIFPAGGKSEI 60
           MEMSSKRDDKKRLKILCLHGFRTNGSFLRKQISKWDPSIFAHFDMEFPDGIFPAGGKSEI
Sbjct: 1   MEMSSKRDDKKRLKILCLHGFRTNGSFLRKQISKWDPSIFAHFDMEFPDGIFPAGGKSEI 60

Query: 61  EGYFPPPYFEWFQFNEDFTEYKNLEECIDYVCNYITTNGPFHGLLGFSQGAVLSALLLGY 120
           EGYFPPPYFEWFQFNEDFTEYKNLEECIDYVCNYITTNGPFHGLLGFSQGAVLSALLLGY
Sbjct: 61  EGYFPPPYFEWFQFNEDFTEYKNLEECIDYVCNYITTNGPFHGLLGFSQGAVLSALLLGY 120

Query: 121 QAQGKVLKEHPPMKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLA 180
           QAQGKVLKEHPPMKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLA
Sbjct: 121 QAQGKVLKEHPPMKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLA 180

Query: 181 SVFHEPLIIRHPQGHTVPRLDESATKELKCWVDAILSSRDHDGDGEVKENVIDKVDERIE 240
           SVFHEPLIIRHPQGHTVPRLDESATKELKCWVDAILSSRDHDGDGEVKENVIDKVDERIE
Sbjct: 181 SVFHEPLIIRHPQGHTVPRLDESATKELKCWVDAILSSRDHDGDGEVKENVIDKVDERIE 240

Query: 241 NQILKGENKCIDVV 254
           NQILKGENKCIDVV
Sbjct: 241 NQILKGENKCIDVV 254

[Close]

BLAST of Cucsat.G12648 vs. ExPASy TrEMBL
Match: A0A1S3B6W5 (esterase C25G4.2-like OS=Cucumis melo OX=3656 GN=LOC103486820 PE=4 SV=1)

HSP 1 Score: 487 bits (1253), Expect = 1.83e-173
Identity = 233/252 (92.46%), Postives = 243/252 (96.43%), Query Frame = 0


Query: 3   MSSKRDDKKRLKILCLHGFRTNGSFLRKQISKWDPSIFAHFDMEFPDGIFPAGGKSEIEG 62
           M S + D++RLKILCLHGFRTNGSFLRKQ+SKWDPSIFA F MEFPDGIFPAGGKSEIEG
Sbjct: 1   MESGKRDEERLKILCLHGFRTNGSFLRKQLSKWDPSIFALFHMEFPDGIFPAGGKSEIEG 60

Query: 63  YFPPPYFEWFQFNEDFTEYKNLEECIDYVCNYITTNGPFHGLLGFSQGAVLSALLLGYQA 122
           YFPPPYFEWFQFNEDFTEYKNLEECIDYVCNYITTNGPFHGLLGFSQGAVLSALLLGYQA
Sbjct: 61  YFPPPYFEWFQFNEDFTEYKNLEECIDYVCNYITTNGPFHGLLGFSQGAVLSALLLGYQA 120

Query: 123 QGKVLKEHPPMKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASV 182
           QGKVL+EHPP+KMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASV
Sbjct: 121 QGKVLREHPPIKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASV 180

Query: 183 FHEPLIIRHPQGHTVPRLDESATKELKCWVDAILSSRDHDGDGEVKENVIDKVDERIENQ 242
           FH+PLIIRHPQGHTVPRLDESATKEL+CWVD ILSSR HDGD EVKE+VIDKVDERIENQ
Sbjct: 181 FHQPLIIRHPQGHTVPRLDESATKELQCWVDTILSSRGHDGDSEVKESVIDKVDERIENQ 240

Query: 243 ILKGENKCIDVV 254
           I+K ENKCIDVV
Sbjct: 241 IMKEENKCIDVV 252

[Close]

BLAST of Cucsat.G12648 vs. ExPASy TrEMBL
Match: A0A6J1DLE8 (dihydrofolate reductase-like OS=Momordica charantia OX=3673 GN=LOC111021534 PE=4 SV=1)

HSP 1 Score: 412 bits (1058), Expect = 9.18e-144
Identity = 196/244 (80.33%), Postives = 222/244 (90.98%), Query Frame = 0


Query: 4   SSKRDDKKRLKILCLHGFRTNGSFLRKQISKWDPSIFAHFDMEFPDGIFPAGGKSEIEGY 63
           S KR++K  +KILCLHGFRT+GSFLRKQ+SKWDPSIFAHFD++FPDG+ PAGGKS+IEGY
Sbjct: 3   SGKRENK--MKILCLHGFRTSGSFLRKQLSKWDPSIFAHFDLDFPDGMCPAGGKSDIEGY 62

Query: 64  FPPPYFEWFQFNEDFTEYKNLEECIDYVCNYITTNGPFHGLLGFSQGAVLSALLLGYQAQ 123
           FPPPYFEWFQFN++FTEY NLE+CI Y+CNYITTNGPF GLLGFSQGA LSALLLGYQAQ
Sbjct: 63  FPPPYFEWFQFNQEFTEYTNLEQCIGYLCNYITTNGPFDGLLGFSQGATLSALLLGYQAQ 122

Query: 124 GKVLKEHPPMKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASVF 183
           GKVLKEHPP+K+ VSISG+KF+DE ICEVAYKHKI+ KSVH IGEKDWLKLPSQ+LAS F
Sbjct: 123 GKVLKEHPPIKLLVSISGSKFRDEKICEVAYKHKIEAKSVHLIGEKDWLKLPSQELASAF 182

Query: 184 HEPLIIRHPQGHTVPRLDESATKELKCWVDAILSSRDHD-GDGEVKEN-VIDKVDERIEN 243
           H+PLIIRHPQGHTVPRLDE+ATKEL+CWVDA+LS  DH   D EVKEN VIDK+DE+IEN
Sbjct: 183 HDPLIIRHPQGHTVPRLDEAATKELQCWVDAVLSGHDHVIEDEEVKENYVIDKMDEKIEN 242

Query: 244 QILK 245
            + K
Sbjct: 243 GLSK 244

[Close]

BLAST of Cucsat.G12648 vs. ExPASy TrEMBL
Match: A0A1R3J7I5 (Serine hydrolase FSH OS=Corchorus capsularis OX=210143 GN=CCACVL1_07293 PE=4 SV=1)

HSP 1 Score: 362 bits (928), Expect = 5.32e-124
Identity = 162/208 (77.88%), Postives = 189/208 (90.87%), Query Frame = 0


Query: 9   DKKRLKILCLHGFRTNGSFLRKQISKWDPSIFAHFDMEFPDGIFPAGGKSEIEGYFPPPY 68
           +K+++KILCLHGFRT+G FL+KQISKWDPSIF HFDM+FPDGIF AGGKSEIEG FPPPY
Sbjct: 4   EKQKMKILCLHGFRTSGKFLQKQISKWDPSIFFHFDMDFPDGIFAAGGKSEIEGIFPPPY 63

Query: 69  FEWFQFNEDFTEYKNLEECIDYVCNYITTNGPFHGLLGFSQGAVLSALLLGYQAQGKVLK 128
           FEWFQFN+DFTEY NL++CI Y+C YI+TNGPFHGLLGFSQGA L ALLLGYQAQGKVLK
Sbjct: 64  FEWFQFNKDFTEYTNLDQCISYLCEYISTNGPFHGLLGFSQGATLCALLLGYQAQGKVLK 123

Query: 129 EHPPMKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASVFHEPLI 188
           EHPP KM +SISG+KF+D +ICEVAYK K+KVKSVHFIG+KDWLKLPS++LA+ F  PLI
Sbjct: 124 EHPPFKMMISISGSKFRDPTICEVAYKEKMKVKSVHFIGDKDWLKLPSEELATAFDNPLI 183

Query: 189 IRHPQGHTVPRLDESATKELKCWVDAIL 216
           IRHPQGHTVPRLDE++T++L+ W+  IL
Sbjct: 184 IRHPQGHTVPRLDETSTQQLRTWITNIL 211

[Close]

BLAST of Cucsat.G12648 vs. ExPASy TrEMBL
Match: A0A022PWU6 (FSH1 domain-containing protein OS=Erythranthe guttata OX=4155 GN=MIMGU_mgv1a012528mg PE=4 SV=1)

HSP 1 Score: 358 bits (918), Expect = 1.71e-122
Identity = 166/209 (79.43%), Postives = 187/209 (89.47%), Query Frame = 0


Query: 3   MSSKRDDKKRLKILCLHGFRTNGSFLRKQISKWDPSIFAHFDMEFPDGIFPAGGKSEIEG 62
           M +K    K+ KILCLHGFRT+GSFL KQISKWDPSIFAHFDM+FPDGIFPAGGKS+IEG
Sbjct: 1   MEAKEVAIKKKKILCLHGFRTSGSFLCKQISKWDPSIFAHFDMDFPDGIFPAGGKSDIEG 60

Query: 63  YFPPPYFEWFQFNEDFTEYKNLEECIDYVCNYITTNGPFHGLLGFSQGAVLSALLLGYQA 122
            FPPPYFEWFQF+++FTEY NL+ECIDY+C+YIT+ GPFHGLLGFSQGA LSALLLGYQA
Sbjct: 61  IFPPPYFEWFQFDKEFTEYTNLDECIDYLCDYITSKGPFHGLLGFSQGATLSALLLGYQA 120

Query: 123 QGKVLKEHPPMKMFVSISGTKFKDESICEVAYKHKIKVKSVHFIGEKDWLKLPSQQLASV 182
           QGK+LKEHPPM++FVSISG+KF+  SICEVAYK  I VKSVHFIGEKDWLKLPS++LAS 
Sbjct: 121 QGKILKEHPPMELFVSISGSKFRQPSICEVAYKDPINVKSVHFIGEKDWLKLPSEELASA 180

Query: 183 FHEPLIIRHPQGHTVPRLDESATKELKCW 211
           F +PLIIRHPQGHTVPRLDE A K+L  W
Sbjct: 181 FVDPLIIRHPQGHTVPRLDEDAVKKLGNW 209

The following BLAST results are available for this feature:

BLAST of Cucsat.G12648 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P36591	2.8e-15	27.18	Dihydrofolate reductase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) O...	[more]
Q99369	1.8e-14	27.24	Family of serine hydrolases 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / ...	[more]
Q7QBJ0	4.0e-14	26.12	Esterase AGAP003155 OS=Anopheles gambiae OX=7165 GN=AGAP003155 PE=3 SV=3	[more]
Q18169	1.5e-13	29.19	Esterase C25G4.2 OS=Caenorhabditis elegans OX=6239 GN=C25G4.2 PE=3 SV=1	[more]
A4II73	2.6e-13	31.55	Esterase OVCA2 OS=Xenopus tropicalis OX=8364 GN=ovca2 PE=2 SV=2	[more]

BLAST of Cucsat.G12648 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004136600.1	2.80e-189	100.00	esterase OVCA2 [Cucumis sativus] >KGN59361.1 hypothetical protein Csa_001052 [Cu...	[more]
XP_008443142.1	3.78e-173	92.46	PREDICTED: esterase C25G4.2-like [Cucumis melo]	[more]
KAG6596208.1	3.40e-152	84.77	Esterase-like protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023539260.1	9.74e-152	85.60	family of serine hydrolases 2-like [Cucurbita pepo subsp. pepo]	[more]
XP_022154224.1	1.90e-143	80.33	dihydrofolate reductase-like [Momordica charantia]	[more]

BLAST of Cucsat.G12648 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LH35	1.36e-189	100.00	FSH1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G813300 PE=4 S...	[more]
A0A1S3B6W5	1.83e-173	92.46	esterase C25G4.2-like OS=Cucumis melo OX=3656 GN=LOC103486820 PE=4 SV=1	[more]
A0A6J1DLE8	9.18e-144	80.33	dihydrofolate reductase-like OS=Momordica charantia OX=3673 GN=LOC111021534 PE=4...	[more]
A0A1R3J7I5	5.32e-124	77.88	Serine hydrolase FSH OS=Corchorus capsularis OX=210143 GN=CCACVL1_07293 PE=4 SV=...	[more]
A0A022PWU6	1.71e-122	79.43	FSH1 domain-containing protein OS=Erythranthe guttata OX=4155 GN=MIMGU_mgv1a0125...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005645	Serine hydrolase FSH	PFAM	PF03959	FSH1	coord: 11..112 e-value: 1.6E-23 score: 83.5
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 9..116 e-value: 3.6E-24 score: 87.5
None	No IPR available	PANTHER	PTHR48070	ESTERASE OVCA2	coord: 7..110
None	No IPR available	PANTHER	PTHR48070:SF10	OS07G0687100 PROTEIN	coord: 7..110

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G12648.T1	Cucsat.G12648.T1	mRNA
Cucsat.G12648.T2	Cucsat.G12648.T2	mRNA
Cucsat.G12648.T3	Cucsat.G12648.T3	mRNA
Cucsat.G12648.T4	Cucsat.G12648.T4	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0016787	hydrolase activity