Spg006645.1 (mRNA) Sponge gourd (cylindrica) v1

Overview


Name	Spg006645.1
Type	mRNA
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Reverse transcriptase
Location	scaffold7: 47434586 .. 47436361 (-)
Sequence length	540
RNA-Seq Expression	Spg006645.1
Synteny	Spg006645.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGAGAAGTTTATGGTTTTTGAAATGGAAAACAGAGCACAGGATTTTCGTGCAAACCAGGAGGATCTCTGGTGTTCTCTGGTGTTTTGAGCATTCTGGGGTGTACAAGTTGAATCAGAATCTCTATTTGGTATGGTTGGAAAGCTGATTCAATTTCCTACAACGTTCATGAAGAATTTGTGAGCCAAAAATGACTTGAAGTAGGTTTATTTTCAGGAGAAAGACAAAGGGCAGCAGAGAACCTCGAATCTGTGTTGTCTGTGTTCGGGGCATTTCTGGACAAATATGGGTTGGAATACCGTTTTCATTTTTTCAGGTAAGTTGTCGAGTGTTCGAAAATGAAGAGTTTGATGTATAGTACATTAATGTTGGTTAATTATTGAGGAAGTTATGGGTGTTGGAAGTTAGGGTATAGAATCTGGAAAATCTGGAAAGTAGAATGTGAATGTGATTCTTAATTCAATTGTTTAGAAATTCATGAGCATGGAAAGACAAAACTAATTATGGTTCTGATGCTCGGTTTTGGTTGGTTGACAGGCCAAGAGGAAATAGGCCGGGTCTATAAACCAGGAAACTAGCTAAGACGGTGAGTGACAAAATGAATTTCGAAAATTGTTTCCAACGGTTGTTCATGTTTAGATGTTTTAAATGCTTTTCTATGAATGATACATGGTTTTTATGAATGGTTTCAAGCTTAAGTTATGAGCTTAGATTTTCTAATGAGGTTTATAAATGAGCACGTGATTATTGAGTTTCTGAAAAATAGTTGAAATGACATCCTTTTAAGCCAAGCATGTATTAGCAAGAATTATCCAAAGATTTTCGGTTTTTCTTCCACGAGCAAGCTGAGTAAACTTGGGTTTTACAAAAGATAACGTTAAGCAGGCATGTTTAAACGTTTTCATGTGATTTTCTAAGATTTCTATTGACTACATGACTGAGATATTGAGCCCGAGGCTATATGCTACCTTGTGCACACAGGTTATATTTCGTTGTTGACGTTGAGTGTACTCCGTGACAACGATGCTGTCGTGAGTGCTGGGCGGGCCCCACTACATCGTAGGACTAGTAAACGTTGGTTGTACTGGGCATGCCCTACACAACGTAGATCGGTCATGTTAGTTAAAATGTTTGATGTACTTGATATGTCTTGCTAGATTTTTTTAATGACGAGCCTGCGATGTTTTTAAGAAAGCTTTTATCGTTGCACGTTTATGTTTAACGCCTTGGTTGAGCATCTTCATACGTTTAAAGTTTTATCACCTGTTCTGATTTCCGAATGTATAGTTTTAAAGTTAGTCACTCACTGGGCTTCGTAGCTCATTCTTTCAAAATGTTTTTCCACTTTCAGGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGCTGAAAGTTCCAGATCAAGCTCCAGTACGTGGGTGGAGTTGTATATTGAGTCTGTCTATATTGTAAATGTTTTGTAAGGATTGGACAATTTAGTCCTAGAGGTCGTGGTTGTATAAACCTCATTTGTATATGTTTTTGGTTGTGAAATCTATATCAGGTTATTTTCCAAATCGAGTTATGTCAGGGCTGCACCTCGTGTATATTAGAAGGTTTTAAGGGATTCCGCTGTGTTGTGTTTCATGTGGAATGTTTTATATCTAAGATTAGTATGTTTATCAGGTTCTACGTTTCAGGAGGGTCAACGGGTATCGTTAGAGGATGACGATGTTCGTTGGCTTCACGCCATCTTCCGGACTAAGCTAGCAGGTGGTCTGGGAGGG

mRNA sequence

ATGATGAGAAGTTTATGGTTTTTGAAATGGAAAACAGAGCACAGGATTTTCGTGCAAACCAGGAGGATCTCTGGTGTTCTCTGGTGTTTTGAGCATTCTGGGGTGTACAAGTTGAATCAGAATCTCTATTTGGAGAAAGACAAAGGGCAGCAGAGAACCTCGAATCTGTGTTGTCTGTGTTCGGGGCATTTCTGGACAAATATGGGTTGGAATACCGTTTTCATTTTTTCAGGTTATATTTCGTTGTTGACGTTGAGTGTACTCCGTGACAACGATGCTGTCGTGAGTGCTGGGCGGGCCCCACTACATCGTAGGACTAGTAAACGTTGGTTGTACTGGGCATGCCCTACACAACGTAGATCGGTCATGTTAGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGCTGAAAGTTCCAGATCAAGCTCCAGTTCTACGTTTCAGGAGGGTCAACGGGTATCGTTAGAGGATGACGATGTTCGTTGGCTTCACGCCATCTTCCGGACTAAGCTAGCAGGTGGTCTGGGAGGG

Coding sequence (CDS)

ATGATGAGAAGTTTATGGTTTTTGAAATGGAAAACAGAGCACAGGATTTTCGTGCAAACCAGGAGGATCTCTGGTGTTCTCTGGTGTTTTGAGCATTCTGGGGTGTACAAGTTGAATCAGAATCTCTATTTGGAGAAAGACAAAGGGCAGCAGAGAACCTCGAATCTGTGTTGTCTGTGTTCGGGGCATTTCTGGACAAATATGGGTTGGAATACCGTTTTCATTTTTTCAGGTTATATTTCGTTGTTGACGTTGAGTGTACTCCGTGACAACGATGCTGTCGTGAGTGCTGGGCGGGCCCCACTACATCGTAGGACTAGTAAACGTTGGTTGTACTGGGCATGCCCTACACAACGTAGATCGGTCATGTTAGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGCTGAAAGTTCCAGATCAAGCTCCAGTTCTACGTTTCAGGAGGGTCAACGGGTATCGTTAGAGGATGACGATGTTCGTTGGCTTCACGCCATCTTCCGGACTAAGCTAGCAGGTGGTCTGGGAGGG

Protein sequence

MMRSLWFLKWKTEHRIFVQTRRISGVLWCFEHSGVYKLNQNLYLEKDKGQQRTSNLCCLCSGHFWTNMGWNTVFIFSGYISLLTLSVLRDNDAVVSAGRAPLHRRTSKRWLYWACPTQRRSVMLVEIELPVPDTLPTSAESSRSSSSSTFQEGQRVSLEDDDVRWLHAIFRTKLAGGLGG

Homology

[Close]

BLAST of Spg006645.1 vs. NCBI nr
Match: KAA0065516.1 (uncharacterized protein E6C27_scaffold638G00090 [Cucumis melo var. makuwa])

HSP 1 Score: 127.5 bits (319), Expect = 1.2e-25
Identity = 86/168 (51.19%), Postives = 93/168 (55.36%), Query Frame = 0


Query: 35  VYKLNQNLYLEKDKGQQRTSNLCCLCSGHFWTNMGWNTVFIFSGYISLLTLSVLRDNDAV 94
           V K NQ LYL   +G     N   L +G     + +          SL TLSVLRDNDAV
Sbjct: 508 VQKSNQKLYLVWPRG-----NRSSLQTGELDKTVRF----------SLSTLSVLRDNDAV 567

Query: 95  VSAG------------RAPLHRRTSKRWLYWACPTQRRSVML----------VEIELPVP 154
           VSAG            RAPLHRRTSKRWLYWACPT RRSVML          VEIEL VP
Sbjct: 568 VSAGGPHYDKDDGNAVRAPLHRRTSKRWLYWACPTPRRSVMLVKTFDVLVMPVEIELSVP 627

Query: 155 DTLPTSAESSRSSSSSTFQEGQRVSLEDDDVRWLHAIFRTKLAGGLGG 181
           DTLPTSAESS S+S S         +  DDV WLH +FR K  GG GG
Sbjct: 628 DTLPTSAESSESNSRSA------GIVRGDDVCWLHVVFRAKTTGGPGG 654

[Close]

BLAST of Spg006645.1 vs. NCBI nr
Match: TYJ98800.1 (uncharacterized protein E5676_scaffold1625G00140 [Cucumis melo var. makuwa])

HSP 1 Score: 127.5 bits (319), Expect = 1.2e-25
Identity = 86/168 (51.19%), Postives = 93/168 (55.36%), Query Frame = 0


Query: 35  VYKLNQNLYLEKDKGQQRTSNLCCLCSGHFWTNMGWNTVFIFSGYISLLTLSVLRDNDAV 94
           V K NQ LYL   +G     N   L +G     + +          SL TLSVLRDNDAV
Sbjct: 94  VQKSNQKLYLVWPRG-----NRSSLQTGELDKTVRF----------SLSTLSVLRDNDAV 153

Query: 95  VSAG------------RAPLHRRTSKRWLYWACPTQRRSVML----------VEIELPVP 154
           VSAG            RAPLHRRTSKRWLYWACPT RRSVML          VEIEL VP
Sbjct: 154 VSAGGPHYDKDDGNAVRAPLHRRTSKRWLYWACPTPRRSVMLVKTFDVLVMPVEIELSVP 213

Query: 155 DTLPTSAESSRSSSSSTFQEGQRVSLEDDDVRWLHAIFRTKLAGGLGG 181
           DTLPTSAESS S+S S         +  DDV WLH +FR K  GG GG
Sbjct: 214 DTLPTSAESSESNSRSA------GIVRGDDVCWLHVVFRAKTTGGPGG 240

[Close]

BLAST of Spg006645.1 vs. NCBI nr
Match: KAA0037581.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 102.1 bits (253), Expect = 5.3e-18
Identity = 83/207 (40.10%), Postives = 97/207 (46.86%), Query Frame = 0


Query: 35  VYKLNQNLYLEKDKGQQRTSNLCCLCSGHFWTN-----MGWNTVFIFSGYIS-------- 94
           V+K NQ LYL +   + R   L CLCSG FW N       +  +++    IS        
Sbjct: 26  VHKSNQKLYLIRSGREPRI--LYCLCSGQFWMNWLRISFSFLQLWVLEVRISNLENLEMP 85

Query: 95  -------------------LLTLSVLRDNDAVVSAGRAPLHRRTSKRWLYWACPT----Q 154
                              L TLSVL DNDAVVSAG+APL +R   RW     PT    +
Sbjct: 86  RGNRLSLQTEEIDKTVIFPLSTLSVLSDNDAVVSAGQAPLRQR---RWECRTGPTTSFFK 145

Query: 155 RRSVMLVEIELPVPDTLPTSAESSRSSSSSTFQ---EGQRVSL----------------- 181
             S  LVEIELPVPD LPTSAESSRS+SS+  +   E   V +                 
Sbjct: 146 MFSTFLVEIELPVPDILPTSAESSRSNSSTWLELYFESVHVEMFYKVILQTKRCQGCILW 205

[Close]

BLAST of Spg006645.1 vs. NCBI nr
Match: TYK03091.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 102.1 bits (253), Expect = 5.3e-18
Identity = 83/207 (40.10%), Postives = 97/207 (46.86%), Query Frame = 0


Query: 35  VYKLNQNLYLEKDKGQQRTSNLCCLCSGHFWTN-----MGWNTVFIFSGYIS-------- 94
           V+K NQ LYL +   + R   L CLCSG FW N       +  +++    IS        
Sbjct: 26  VHKSNQKLYLIRSGREPRI--LYCLCSGQFWMNWLRISFSFLQLWVLEVRISNLENLEMP 85

Query: 95  -------------------LLTLSVLRDNDAVVSAGRAPLHRRTSKRWLYWACPT----Q 154
                              L TLSVL DNDAVVSAG+APL +R   RW     PT    +
Sbjct: 86  RGNRLSLQTEEIDKTVIFPLSTLSVLSDNDAVVSAGQAPLRQR---RWECRTGPTTSFFK 145

Query: 155 RRSVMLVEIELPVPDTLPTSAESSRSSSSSTFQ---EGQRVSL----------------- 181
             S  LVEIELPVPD LPTSAESSRS+SS+  +   E   V +                 
Sbjct: 146 MFSTFLVEIELPVPDILPTSAESSRSNSSTWLELYFESVHVEMFYKVILQTKRCQGCILW 205

[Close]

BLAST of Spg006645.1 vs. NCBI nr
Match: KAA0041324.1 (DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 84.3 bits (207), Expect = 1.1e-12
Identity = 43/61 (70.49%), Postives = 45/61 (73.77%), Query Frame = 0


Query: 78  GYISLLTLSVLRDNDAVV------------SAGRAPLHRRTSKRWLYWACPTQRRSVMLV 127
           GYISLLTLSVLRDN+ VV            SAGR PLHRRTSKRWLYWACPTQR  VMLV
Sbjct: 770 GYISLLTLSVLRDNNVVVSVERVPLVKDDGSAGRTPLHRRTSKRWLYWACPTQRNEVMLV 829

[Close]

BLAST of Spg006645.1 vs. ExPASy TrEMBL
Match: A0A5A7VGQ5 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold638G00090 PE=4 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 5.7e-26
Identity = 86/168 (51.19%), Postives = 93/168 (55.36%), Query Frame = 0


Query: 35  VYKLNQNLYLEKDKGQQRTSNLCCLCSGHFWTNMGWNTVFIFSGYISLLTLSVLRDNDAV 94
           V K NQ LYL   +G     N   L +G     + +          SL TLSVLRDNDAV
Sbjct: 508 VQKSNQKLYLVWPRG-----NRSSLQTGELDKTVRF----------SLSTLSVLRDNDAV 567

Query: 95  VSAG------------RAPLHRRTSKRWLYWACPTQRRSVML----------VEIELPVP 154
           VSAG            RAPLHRRTSKRWLYWACPT RRSVML          VEIEL VP
Sbjct: 568 VSAGGPHYDKDDGNAVRAPLHRRTSKRWLYWACPTPRRSVMLVKTFDVLVMPVEIELSVP 627

Query: 155 DTLPTSAESSRSSSSSTFQEGQRVSLEDDDVRWLHAIFRTKLAGGLGG 181
           DTLPTSAESS S+S S         +  DDV WLH +FR K  GG GG
Sbjct: 628 DTLPTSAESSESNSRSA------GIVRGDDVCWLHVVFRAKTTGGPGG 654

[Close]

BLAST of Spg006645.1 vs. ExPASy TrEMBL
Match: A0A5D3BG85 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1625G00140 PE=4 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 5.7e-26
Identity = 86/168 (51.19%), Postives = 93/168 (55.36%), Query Frame = 0


Query: 35  VYKLNQNLYLEKDKGQQRTSNLCCLCSGHFWTNMGWNTVFIFSGYISLLTLSVLRDNDAV 94
           V K NQ LYL   +G     N   L +G     + +          SL TLSVLRDNDAV
Sbjct: 94  VQKSNQKLYLVWPRG-----NRSSLQTGELDKTVRF----------SLSTLSVLRDNDAV 153

Query: 95  VSAG------------RAPLHRRTSKRWLYWACPTQRRSVML----------VEIELPVP 154
           VSAG            RAPLHRRTSKRWLYWACPT RRSVML          VEIEL VP
Sbjct: 154 VSAGGPHYDKDDGNAVRAPLHRRTSKRWLYWACPTPRRSVMLVKTFDVLVMPVEIELSVP 213

Query: 155 DTLPTSAESSRSSSSSTFQEGQRVSLEDDDVRWLHAIFRTKLAGGLGG 181
           DTLPTSAESS S+S S         +  DDV WLH +FR K  GG GG
Sbjct: 214 DTLPTSAESSESNSRSA------GIVRGDDVCWLHVVFRAKTTGGPGG 240

[Close]

BLAST of Spg006645.1 vs. ExPASy TrEMBL
Match: A0A5D3BTP3 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold374G00020 PE=4 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 2.6e-18
Identity = 83/207 (40.10%), Postives = 97/207 (46.86%), Query Frame = 0


Query: 35  VYKLNQNLYLEKDKGQQRTSNLCCLCSGHFWTN-----MGWNTVFIFSGYIS-------- 94
           V+K NQ LYL +   + R   L CLCSG FW N       +  +++    IS        
Sbjct: 26  VHKSNQKLYLIRSGREPRI--LYCLCSGQFWMNWLRISFSFLQLWVLEVRISNLENLEMP 85

Query: 95  -------------------LLTLSVLRDNDAVVSAGRAPLHRRTSKRWLYWACPT----Q 154
                              L TLSVL DNDAVVSAG+APL +R   RW     PT    +
Sbjct: 86  RGNRLSLQTEEIDKTVIFPLSTLSVLSDNDAVVSAGQAPLRQR---RWECRTGPTTSFFK 145

Query: 155 RRSVMLVEIELPVPDTLPTSAESSRSSSSSTFQ---EGQRVSL----------------- 181
             S  LVEIELPVPD LPTSAESSRS+SS+  +   E   V +                 
Sbjct: 146 MFSTFLVEIELPVPDILPTSAESSRSNSSTWLELYFESVHVEMFYKVILQTKRCQGCILW 205

[Close]

BLAST of Spg006645.1 vs. ExPASy TrEMBL
Match: A0A5A7T7M6 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold277G002250 PE=4 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 2.6e-18
Identity = 83/207 (40.10%), Postives = 97/207 (46.86%), Query Frame = 0


Query: 35  VYKLNQNLYLEKDKGQQRTSNLCCLCSGHFWTN-----MGWNTVFIFSGYIS-------- 94
           V+K NQ LYL +   + R   L CLCSG FW N       +  +++    IS        
Sbjct: 26  VHKSNQKLYLIRSGREPRI--LYCLCSGQFWMNWLRISFSFLQLWVLEVRISNLENLEMP 85

Query: 95  -------------------LLTLSVLRDNDAVVSAGRAPLHRRTSKRWLYWACPT----Q 154
                              L TLSVL DNDAVVSAG+APL +R   RW     PT    +
Sbjct: 86  RGNRLSLQTEEIDKTVIFPLSTLSVLSDNDAVVSAGQAPLRQR---RWECRTGPTTSFFK 145

Query: 155 RRSVMLVEIELPVPDTLPTSAESSRSSSSSTFQ---EGQRVSL----------------- 181
             S  LVEIELPVPD LPTSAESSRS+SS+  +   E   V +                 
Sbjct: 146 MFSTFLVEIELPVPDILPTSAESSRSNSSTWLELYFESVHVEMFYKVILQTKRCQGCILW 205

[Close]

BLAST of Spg006645.1 vs. ExPASy TrEMBL
Match: A0A5A7TCY8 (DNA/RNA polymerases superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold128G003250 PE=4 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 5.5e-13
Identity = 43/61 (70.49%), Postives = 45/61 (73.77%), Query Frame = 0


Query: 78  GYISLLTLSVLRDNDAVV------------SAGRAPLHRRTSKRWLYWACPTQRRSVMLV 127
           GYISLLTLSVLRDN+ VV            SAGR PLHRRTSKRWLYWACPTQR  VMLV
Sbjct: 770 GYISLLTLSVLRDNNVVVSVERVPLVKDDGSAGRTPLHRRTSKRWLYWACPTQRNEVMLV 829

The following BLAST results are available for this feature:

BLAST of Spg006645.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0065516.1	1.2e-25	51.19	uncharacterized protein E6C27_scaffold638G00090 [Cucumis melo var. makuwa]	[more]
TYJ98800.1	1.2e-25	51.19	uncharacterized protein E5676_scaffold1625G00140 [Cucumis melo var. makuwa]	[more]
KAA0037581.1	5.3e-18	40.10	reverse transcriptase [Cucumis melo var. makuwa]	[more]
TYK03091.1	5.3e-18	40.10	reverse transcriptase [Cucumis melo var. makuwa]	[more]
KAA0041324.1	1.1e-12	70.49	DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa]	[more]

BLAST of Spg006645.1 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Spg006645.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VGQ5	5.7e-26	51.19	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]
A0A5D3BG85	5.7e-26	51.19	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A5D3BTP3	2.6e-18	40.10	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold37...	[more]
A0A5A7T7M6	2.6e-18	40.10	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold27...	[more]
A0A5A7TCY8	5.5e-13	70.49	DNA/RNA polymerases superfamily protein OS=Cucumis melo var. makuwa OX=1194695 G...	[more]

BLAST of Spg006645.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 135..155
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 135..154

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spg006645	Spg006645	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg006645.1-cds	Spg006645.1-cds-scaffold7:47434586..47434686	CDS
Spg006645.1-cds	Spg006645.1-cds-scaffold7:47434942..47435008	CDS
Spg006645.1-cds	Spg006645.1-cds-scaffold7:47435239..47435378	CDS
Spg006645.1-cds	Spg006645.1-cds-scaffold7:47436044..47436143	CDS
Spg006645.1-cds	Spg006645.1-cds-scaffold7:47436230..47436361	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spg006645.1	Spg006645.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003824	catalytic activity