Spg006645 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg006645
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionReverse transcriptase
Locationscaffold7: 47434586 .. 47436361 (-)
RNA-Seq ExpressionSpg006645
SyntenySpg006645
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGAGAAGTTTATGGTTTTTGAAATGGAAAACAGAGCACAGGATTTTCGTGCAAACCAGGAGGATCTCTGGTGTTCTCTGGTGTTTTGAGCATTCTGGGGTGTACAAGTTGAATCAGAATCTCTATTTGGTATGGTTGGAAAGCTGATTCAATTTCCTACAACGTTCATGAAGAATTTGTGAGCCAAAAATGACTTGAAGTAGGTTTATTTTCAGGAGAAAGACAAAGGGCAGCAGAGAACCTCGAATCTGTGTTGTCTGTGTTCGGGGCATTTCTGGACAAATATGGGTTGGAATACCGTTTTCATTTTTTCAGGTAAGTTGTCGAGTGTTCGAAAATGAAGAGTTTGATGTATAGTACATTAATGTTGGTTAATTATTGAGGAAGTTATGGGTGTTGGAAGTTAGGGTATAGAATCTGGAAAATCTGGAAAGTAGAATGTGAATGTGATTCTTAATTCAATTGTTTAGAAATTCATGAGCATGGAAAGACAAAACTAATTATGGTTCTGATGCTCGGTTTTGGTTGGTTGACAGGCCAAGAGGAAATAGGCCGGGTCTATAAACCAGGAAACTAGCTAAGACGGTGAGTGACAAAATGAATTTCGAAAATTGTTTCCAACGGTTGTTCATGTTTAGATGTTTTAAATGCTTTTCTATGAATGATACATGGTTTTTATGAATGGTTTCAAGCTTAAGTTATGAGCTTAGATTTTCTAATGAGGTTTATAAATGAGCACGTGATTATTGAGTTTCTGAAAAATAGTTGAAATGACATCCTTTTAAGCCAAGCATGTATTAGCAAGAATTATCCAAAGATTTTCGGTTTTTCTTCCACGAGCAAGCTGAGTAAACTTGGGTTTTACAAAAGATAACGTTAAGCAGGCATGTTTAAACGTTTTCATGTGATTTTCTAAGATTTCTATTGACTACATGACTGAGATATTGAGCCCGAGGCTATATGCTACCTTGTGCACACAGGTTATATTTCGTTGTTGACGTTGAGTGTACTCCGTGACAACGATGCTGTCGTGAGTGCTGGGCGGGCCCCACTACATCGTAGGACTAGTAAACGTTGGTTGTACTGGGCATGCCCTACACAACGTAGATCGGTCATGTTAGTTAAAATGTTTGATGTACTTGATATGTCTTGCTAGATTTTTTTAATGACGAGCCTGCGATGTTTTTAAGAAAGCTTTTATCGTTGCACGTTTATGTTTAACGCCTTGGTTGAGCATCTTCATACGTTTAAAGTTTTATCACCTGTTCTGATTTCCGAATGTATAGTTTTAAAGTTAGTCACTCACTGGGCTTCGTAGCTCATTCTTTCAAAATGTTTTTCCACTTTCAGGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGCTGAAAGTTCCAGATCAAGCTCCAGTACGTGGGTGGAGTTGTATATTGAGTCTGTCTATATTGTAAATGTTTTGTAAGGATTGGACAATTTAGTCCTAGAGGTCGTGGTTGTATAAACCTCATTTGTATATGTTTTTGGTTGTGAAATCTATATCAGGTTATTTTCCAAATCGAGTTATGTCAGGGCTGCACCTCGTGTATATTAGAAGGTTTTAAGGGATTCCGCTGTGTTGTGTTTCATGTGGAATGTTTTATATCTAAGATTAGTATGTTTATCAGGTTCTACGTTTCAGGAGGGTCAACGGGTATCGTTAGAGGATGACGATGTTCGTTGGCTTCACGCCATCTTCCGGACTAAGCTAGCAGGTGGTCTGGGAGGG

mRNA sequence

ATGATGAGAAGTTTATGGTTTTTGAAATGGAAAACAGAGCACAGGATTTTCGTGCAAACCAGGAGGATCTCTGGTGTTCTCTGGTGTTTTGAGCATTCTGGGGTGTACAAGTTGAATCAGAATCTCTATTTGGAGAAAGACAAAGGGCAGCAGAGAACCTCGAATCTGTGTTGTCTGTGTTCGGGGCATTTCTGGACAAATATGGGTTGGAATACCGTTTTCATTTTTTCAGGTTATATTTCGTTGTTGACGTTGAGTGTACTCCGTGACAACGATGCTGTCGTGAGTGCTGGGCGGGCCCCACTACATCGTAGGACTAGTAAACGTTGGTTGTACTGGGCATGCCCTACACAACGTAGATCGGTCATGTTAGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGCTGAAAGTTCCAGATCAAGCTCCAGTTCTACGTTTCAGGAGGGTCAACGGGTATCGTTAGAGGATGACGATGTTCGTTGGCTTCACGCCATCTTCCGGACTAAGCTAGCAGGTGGTCTGGGAGGG

Coding sequence (CDS)

ATGATGAGAAGTTTATGGTTTTTGAAATGGAAAACAGAGCACAGGATTTTCGTGCAAACCAGGAGGATCTCTGGTGTTCTCTGGTGTTTTGAGCATTCTGGGGTGTACAAGTTGAATCAGAATCTCTATTTGGAGAAAGACAAAGGGCAGCAGAGAACCTCGAATCTGTGTTGTCTGTGTTCGGGGCATTTCTGGACAAATATGGGTTGGAATACCGTTTTCATTTTTTCAGGTTATATTTCGTTGTTGACGTTGAGTGTACTCCGTGACAACGATGCTGTCGTGAGTGCTGGGCGGGCCCCACTACATCGTAGGACTAGTAAACGTTGGTTGTACTGGGCATGCCCTACACAACGTAGATCGGTCATGTTAGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGCTGAAAGTTCCAGATCAAGCTCCAGTTCTACGTTTCAGGAGGGTCAACGGGTATCGTTAGAGGATGACGATGTTCGTTGGCTTCACGCCATCTTCCGGACTAAGCTAGCAGGTGGTCTGGGAGGG

Protein sequence

MMRSLWFLKWKTEHRIFVQTRRISGVLWCFEHSGVYKLNQNLYLEKDKGQQRTSNLCCLCSGHFWTNMGWNTVFIFSGYISLLTLSVLRDNDAVVSAGRAPLHRRTSKRWLYWACPTQRRSVMLVEIELPVPDTLPTSAESSRSSSSSTFQEGQRVSLEDDDVRWLHAIFRTKLAGGLGG
Homology
BLAST of Spg006645 vs. NCBI nr
Match: KAA0065516.1 (uncharacterized protein E6C27_scaffold638G00090 [Cucumis melo var. makuwa])

HSP 1 Score: 127.5 bits (319), Expect = 1.2e-25
Identity = 86/168 (51.19%), Postives = 93/168 (55.36%), Query Frame = 0

Query: 35  VYKLNQNLYLEKDKGQQRTSNLCCLCSGHFWTNMGWNTVFIFSGYISLLTLSVLRDNDAV 94
           V K NQ LYL   +G     N   L +G     + +          SL TLSVLRDNDAV
Sbjct: 508 VQKSNQKLYLVWPRG-----NRSSLQTGELDKTVRF----------SLSTLSVLRDNDAV 567

Query: 95  VSAG------------RAPLHRRTSKRWLYWACPTQRRSVML----------VEIELPVP 154
           VSAG            RAPLHRRTSKRWLYWACPT RRSVML          VEIEL VP
Sbjct: 568 VSAGGPHYDKDDGNAVRAPLHRRTSKRWLYWACPTPRRSVMLVKTFDVLVMPVEIELSVP 627

Query: 155 DTLPTSAESSRSSSSSTFQEGQRVSLEDDDVRWLHAIFRTKLAGGLGG 181
           DTLPTSAESS S+S S         +  DDV WLH +FR K  GG GG
Sbjct: 628 DTLPTSAESSESNSRSA------GIVRGDDVCWLHVVFRAKTTGGPGG 654

BLAST of Spg006645 vs. NCBI nr
Match: TYJ98800.1 (uncharacterized protein E5676_scaffold1625G00140 [Cucumis melo var. makuwa])

HSP 1 Score: 127.5 bits (319), Expect = 1.2e-25
Identity = 86/168 (51.19%), Postives = 93/168 (55.36%), Query Frame = 0

Query: 35  VYKLNQNLYLEKDKGQQRTSNLCCLCSGHFWTNMGWNTVFIFSGYISLLTLSVLRDNDAV 94
           V K NQ LYL   +G     N   L +G     + +          SL TLSVLRDNDAV
Sbjct: 94  VQKSNQKLYLVWPRG-----NRSSLQTGELDKTVRF----------SLSTLSVLRDNDAV 153

Query: 95  VSAG------------RAPLHRRTSKRWLYWACPTQRRSVML----------VEIELPVP 154
           VSAG            RAPLHRRTSKRWLYWACPT RRSVML          VEIEL VP
Sbjct: 154 VSAGGPHYDKDDGNAVRAPLHRRTSKRWLYWACPTPRRSVMLVKTFDVLVMPVEIELSVP 213

Query: 155 DTLPTSAESSRSSSSSTFQEGQRVSLEDDDVRWLHAIFRTKLAGGLGG 181
           DTLPTSAESS S+S S         +  DDV WLH +FR K  GG GG
Sbjct: 214 DTLPTSAESSESNSRSA------GIVRGDDVCWLHVVFRAKTTGGPGG 240

BLAST of Spg006645 vs. NCBI nr
Match: KAA0037581.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 102.1 bits (253), Expect = 5.3e-18
Identity = 83/207 (40.10%), Postives = 97/207 (46.86%), Query Frame = 0

Query: 35  VYKLNQNLYLEKDKGQQRTSNLCCLCSGHFWTN-----MGWNTVFIFSGYIS-------- 94
           V+K NQ LYL +   + R   L CLCSG FW N       +  +++    IS        
Sbjct: 26  VHKSNQKLYLIRSGREPRI--LYCLCSGQFWMNWLRISFSFLQLWVLEVRISNLENLEMP 85

Query: 95  -------------------LLTLSVLRDNDAVVSAGRAPLHRRTSKRWLYWACPT----Q 154
                              L TLSVL DNDAVVSAG+APL +R   RW     PT    +
Sbjct: 86  RGNRLSLQTEEIDKTVIFPLSTLSVLSDNDAVVSAGQAPLRQR---RWECRTGPTTSFFK 145

Query: 155 RRSVMLVEIELPVPDTLPTSAESSRSSSSSTFQ---EGQRVSL----------------- 181
             S  LVEIELPVPD LPTSAESSRS+SS+  +   E   V +                 
Sbjct: 146 MFSTFLVEIELPVPDILPTSAESSRSNSSTWLELYFESVHVEMFYKVILQTKRCQGCILW 205

BLAST of Spg006645 vs. NCBI nr
Match: TYK03091.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 102.1 bits (253), Expect = 5.3e-18
Identity = 83/207 (40.10%), Postives = 97/207 (46.86%), Query Frame = 0

Query: 35  VYKLNQNLYLEKDKGQQRTSNLCCLCSGHFWTN-----MGWNTVFIFSGYIS-------- 94
           V+K NQ LYL +   + R   L CLCSG FW N       +  +++    IS        
Sbjct: 26  VHKSNQKLYLIRSGREPRI--LYCLCSGQFWMNWLRISFSFLQLWVLEVRISNLENLEMP 85

Query: 95  -------------------LLTLSVLRDNDAVVSAGRAPLHRRTSKRWLYWACPT----Q 154
                              L TLSVL DNDAVVSAG+APL +R   RW     PT    +
Sbjct: 86  RGNRLSLQTEEIDKTVIFPLSTLSVLSDNDAVVSAGQAPLRQR---RWECRTGPTTSFFK 145

Query: 155 RRSVMLVEIELPVPDTLPTSAESSRSSSSSTFQ---EGQRVSL----------------- 181
             S  LVEIELPVPD LPTSAESSRS+SS+  +   E   V +                 
Sbjct: 146 MFSTFLVEIELPVPDILPTSAESSRSNSSTWLELYFESVHVEMFYKVILQTKRCQGCILW 205

BLAST of Spg006645 vs. NCBI nr
Match: KAA0041324.1 (DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 84.3 bits (207), Expect = 1.1e-12
Identity = 43/61 (70.49%), Postives = 45/61 (73.77%), Query Frame = 0

Query: 78  GYISLLTLSVLRDNDAVV------------SAGRAPLHRRTSKRWLYWACPTQRRSVMLV 127
           GYISLLTLSVLRDN+ VV            SAGR PLHRRTSKRWLYWACPTQR  VMLV
Sbjct: 770 GYISLLTLSVLRDNNVVVSVERVPLVKDDGSAGRTPLHRRTSKRWLYWACPTQRNEVMLV 829

BLAST of Spg006645 vs. ExPASy TrEMBL
Match: A0A5A7VGQ5 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold638G00090 PE=4 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 5.7e-26
Identity = 86/168 (51.19%), Postives = 93/168 (55.36%), Query Frame = 0

Query: 35  VYKLNQNLYLEKDKGQQRTSNLCCLCSGHFWTNMGWNTVFIFSGYISLLTLSVLRDNDAV 94
           V K NQ LYL   +G     N   L +G     + +          SL TLSVLRDNDAV
Sbjct: 508 VQKSNQKLYLVWPRG-----NRSSLQTGELDKTVRF----------SLSTLSVLRDNDAV 567

Query: 95  VSAG------------RAPLHRRTSKRWLYWACPTQRRSVML----------VEIELPVP 154
           VSAG            RAPLHRRTSKRWLYWACPT RRSVML          VEIEL VP
Sbjct: 568 VSAGGPHYDKDDGNAVRAPLHRRTSKRWLYWACPTPRRSVMLVKTFDVLVMPVEIELSVP 627

Query: 155 DTLPTSAESSRSSSSSTFQEGQRVSLEDDDVRWLHAIFRTKLAGGLGG 181
           DTLPTSAESS S+S S         +  DDV WLH +FR K  GG GG
Sbjct: 628 DTLPTSAESSESNSRSA------GIVRGDDVCWLHVVFRAKTTGGPGG 654

BLAST of Spg006645 vs. ExPASy TrEMBL
Match: A0A5D3BG85 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1625G00140 PE=4 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 5.7e-26
Identity = 86/168 (51.19%), Postives = 93/168 (55.36%), Query Frame = 0

Query: 35  VYKLNQNLYLEKDKGQQRTSNLCCLCSGHFWTNMGWNTVFIFSGYISLLTLSVLRDNDAV 94
           V K NQ LYL   +G     N   L +G     + +          SL TLSVLRDNDAV
Sbjct: 94  VQKSNQKLYLVWPRG-----NRSSLQTGELDKTVRF----------SLSTLSVLRDNDAV 153

Query: 95  VSAG------------RAPLHRRTSKRWLYWACPTQRRSVML----------VEIELPVP 154
           VSAG            RAPLHRRTSKRWLYWACPT RRSVML          VEIEL VP
Sbjct: 154 VSAGGPHYDKDDGNAVRAPLHRRTSKRWLYWACPTPRRSVMLVKTFDVLVMPVEIELSVP 213

Query: 155 DTLPTSAESSRSSSSSTFQEGQRVSLEDDDVRWLHAIFRTKLAGGLGG 181
           DTLPTSAESS S+S S         +  DDV WLH +FR K  GG GG
Sbjct: 214 DTLPTSAESSESNSRSA------GIVRGDDVCWLHVVFRAKTTGGPGG 240

BLAST of Spg006645 vs. ExPASy TrEMBL
Match: A0A5D3BTP3 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold374G00020 PE=4 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 2.6e-18
Identity = 83/207 (40.10%), Postives = 97/207 (46.86%), Query Frame = 0

Query: 35  VYKLNQNLYLEKDKGQQRTSNLCCLCSGHFWTN-----MGWNTVFIFSGYIS-------- 94
           V+K NQ LYL +   + R   L CLCSG FW N       +  +++    IS        
Sbjct: 26  VHKSNQKLYLIRSGREPRI--LYCLCSGQFWMNWLRISFSFLQLWVLEVRISNLENLEMP 85

Query: 95  -------------------LLTLSVLRDNDAVVSAGRAPLHRRTSKRWLYWACPT----Q 154
                              L TLSVL DNDAVVSAG+APL +R   RW     PT    +
Sbjct: 86  RGNRLSLQTEEIDKTVIFPLSTLSVLSDNDAVVSAGQAPLRQR---RWECRTGPTTSFFK 145

Query: 155 RRSVMLVEIELPVPDTLPTSAESSRSSSSSTFQ---EGQRVSL----------------- 181
             S  LVEIELPVPD LPTSAESSRS+SS+  +   E   V +                 
Sbjct: 146 MFSTFLVEIELPVPDILPTSAESSRSNSSTWLELYFESVHVEMFYKVILQTKRCQGCILW 205

BLAST of Spg006645 vs. ExPASy TrEMBL
Match: A0A5A7T7M6 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold277G002250 PE=4 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 2.6e-18
Identity = 83/207 (40.10%), Postives = 97/207 (46.86%), Query Frame = 0

Query: 35  VYKLNQNLYLEKDKGQQRTSNLCCLCSGHFWTN-----MGWNTVFIFSGYIS-------- 94
           V+K NQ LYL +   + R   L CLCSG FW N       +  +++    IS        
Sbjct: 26  VHKSNQKLYLIRSGREPRI--LYCLCSGQFWMNWLRISFSFLQLWVLEVRISNLENLEMP 85

Query: 95  -------------------LLTLSVLRDNDAVVSAGRAPLHRRTSKRWLYWACPT----Q 154
                              L TLSVL DNDAVVSAG+APL +R   RW     PT    +
Sbjct: 86  RGNRLSLQTEEIDKTVIFPLSTLSVLSDNDAVVSAGQAPLRQR---RWECRTGPTTSFFK 145

Query: 155 RRSVMLVEIELPVPDTLPTSAESSRSSSSSTFQ---EGQRVSL----------------- 181
             S  LVEIELPVPD LPTSAESSRS+SS+  +   E   V +                 
Sbjct: 146 MFSTFLVEIELPVPDILPTSAESSRSNSSTWLELYFESVHVEMFYKVILQTKRCQGCILW 205

BLAST of Spg006645 vs. ExPASy TrEMBL
Match: A0A5A7TCY8 (DNA/RNA polymerases superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold128G003250 PE=4 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 5.5e-13
Identity = 43/61 (70.49%), Postives = 45/61 (73.77%), Query Frame = 0

Query: 78  GYISLLTLSVLRDNDAVV------------SAGRAPLHRRTSKRWLYWACPTQRRSVMLV 127
           GYISLLTLSVLRDN+ VV            SAGR PLHRRTSKRWLYWACPTQR  VMLV
Sbjct: 770 GYISLLTLSVLRDNNVVVSVERVPLVKDDGSAGRTPLHRRTSKRWLYWACPTQRNEVMLV 829

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0065516.11.2e-2551.19uncharacterized protein E6C27_scaffold638G00090 [Cucumis melo var. makuwa][more]
TYJ98800.11.2e-2551.19uncharacterized protein E5676_scaffold1625G00140 [Cucumis melo var. makuwa][more]
KAA0037581.15.3e-1840.10reverse transcriptase [Cucumis melo var. makuwa][more]
TYK03091.15.3e-1840.10reverse transcriptase [Cucumis melo var. makuwa][more]
KAA0041324.11.1e-1270.49DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5A7VGQ55.7e-2651.19Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A5D3BG855.7e-2651.19Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A5D3BTP32.6e-1840.10Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold37... [more]
A0A5A7T7M62.6e-1840.10Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold27... [more]
A0A5A7TCY85.5e-1370.49DNA/RNA polymerases superfamily protein OS=Cucumis melo var. makuwa OX=1194695 G... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 135..155
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 135..154

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg006645.1Spg006645.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003824 catalytic activity