MS004887.1 (mRNA) Bitter gourd (TR) v1

BLAST of MS004887.1 vs. NCBI nr
Match: XP_022140639.1 (uncharacterized protein LOC111011249 [Momordica charantia])

HSP 1 Score: 258.8 bits (660), Expect = 2.8e-65
Identity = 139/147 (94.56%), Postives = 140/147 (95.24%), Query Frame = 0

Query: 1   MGNCLRSNRVMAQDEACSPSPNSSLTETNYKVEDKPAAGSALARPKTEEARIAARGKKVV 60
           MGNCLR+NRVMAQDEACSPSPNSSLTETNYKVEDKPAAGSALARPKTEEARIAAR KKVV
Sbjct: 43  MGNCLRNNRVMAQDEACSPSPNSSLTETNYKVEDKPAAGSALARPKTEEARIAARRKKVV 102

Query: 61  RFQLREDEISGGGGGGGGGVLRIKVVVSQKELKQILKDRESNSSTLEELLAELKMKGRTI 120
           RFQ REDEIS    GGGGGVLRIKVVVSQKELKQILKDRESNSSTLEELLAELKMKGRTI
Sbjct: 103 RFQQREDEIS----GGGGGVLRIKVVVSQKELKQILKDRESNSSTLEELLAELKMKGRTI 162

Query: 121 SDARADKEEDENGSWRPALESIPEDLH 148
           SDARAD EEDENGSWRPALESIPEDLH
Sbjct: 163 SDARADNEEDENGSWRPALESIPEDLH 185

BLAST of MS004887.1 vs. NCBI nr
Match: KAG6570883.1 (hypothetical protein SDJN03_29798, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 161.0 bits (406), Expect = 7.8e-36
Identity = 97/153 (63.40%), Postives = 118/153 (77.12%), Query Frame = 0

Query: 1   MGN-CLRSNRVMAQDEACSPSPNSSLTETNYKVEDKPAAGSALARPKTEEARI-AARGKK 60
           MGN C +SN+VMAQDE+C    NS   E   KVE+KP AGSA+A+PKT E R  AA GKK
Sbjct: 1   MGNSCFKSNKVMAQDESCLALSNSPPVEAK-KVEEKPVAGSAMAKPKTAEERSGAAAGKK 60

Query: 61  VVRFQLREDEISGGGGGGGG---GVLRIKVVVSQKELKQILKDRESNSSTLEELLAELKM 120
           VVRF+L+E++ + GG GG G   GVLRIKVV+SQ+ELKQILK+ E++S +LEEL+AE K+
Sbjct: 61  VVRFKLQEEDENSGGSGGDGDRAGVLRIKVVMSQRELKQILKENENSSRSLEELIAEFKV 120

Query: 121 KGR-TISDARADKEEDENGSWRPALESIPEDLH 148
           KGR T+SDA  D+ EDENGS RPALE IPE LH
Sbjct: 121 KGRTTVSDAITDEVEDENGSRRPALECIPEGLH 152

BLAST of MS004887.1 vs. NCBI nr
Match: XP_038902397.1 (uncharacterized protein LOC120089037 [Benincasa hispida])

HSP 1 Score: 149.8 bits (377), Expect = 1.8e-32
Identity = 89/144 (61.81%), Postives = 103/144 (71.53%), Query Frame = 0

Query: 3   NCLRSNRVMAQDEACSPSPNSSLTETN-YKVEDKPAAGSALARPKTEEARIAARGKKVVR 62
           NC +SN+VMAQDE     P   L      KVE+KP  GSA+A+PKT EAR     KKVVR
Sbjct: 4   NCFKSNKVMAQDE-----PEDLLPPIEAKKVEEKPRPGSAMAKPKTAEARTGGASKKVVR 63

Query: 63  FQLREDEISGGGGGGGGGVLRIKVVVSQKELKQILKDRESNSSTLEELLAELKMKGR-TI 122
           F+L+E+E       G GGVLRIKVV+SQKELKQ+LKDRE+NS TLEEL+ ELK+KGR TI
Sbjct: 64  FKLQEEEEK---NSGDGGVLRIKVVMSQKELKQMLKDRENNSCTLEELITELKVKGRTTI 123

Query: 123 SDARADKEEDENGSWRPALESIPE 145
           SD R D  EDENG W+P LE IPE
Sbjct: 124 SDGRIDAVEDENGRWKPDLEGIPE 139

BLAST of MS004887.1 vs. NCBI nr
Match: KGN63254.1 (hypothetical protein Csa_022493 [Cucumis sativus])

HSP 1 Score: 125.6 bits (314), Expect = 3.6e-25
Identity = 81/148 (54.73%), Postives = 97/148 (65.54%), Query Frame = 0

Query: 1   MGN-CLRSNRVMAQDEACSPSPNSSLTETNYKVEDKPAAGSALARPKTEEARIAARGKKV 60
           MGN C +SN+VMAQD++    P   L E   KV+ +P  GSA+A+PK       A GKKV
Sbjct: 1   MGNICFKSNKVMAQDDSYDDFPPHHLIEPK-KVQQQPLPGSAMAKPKNGTG--GAAGKKV 60

Query: 61  VRFQLRE---DEISGGGGGGGGGVLRIKVVVSQKELKQILKDRESNSSTLEELLAELKMK 120
           VRF L+E   DE     G  G GVLRIKVV+SQKELKQILK RE+NS +LEEL+ ELK+K
Sbjct: 61  VRFNLQEEEKDEEGRNSGDSGPGVLRIKVVISQKELKQILKSRENNSCSLEELIEELKVK 120

Query: 121 GRTISDARADKEEDENGSWRPALESIPE 145
           GR  +        DE GSW+PALE IPE
Sbjct: 121 GRATT-----VSADETGSWKPALECIPE 140

BLAST of MS004887.1 vs. NCBI nr
Match: TYK24218.1 (hypothetical protein E5676_scaffold27G00200 [Cucumis melo var. makuwa])

HSP 1 Score: 121.7 bits (304), Expect = 5.3e-24
Identity = 83/156 (53.21%), Postives = 101/156 (64.74%), Query Frame = 0

Query: 1   MGN-CLRSNRVMAQDEACSPSPNSSLTETNYKVEDKP-AAGSALARPKTEEARIAARGKK 60
           MGN C R+N+VMAQD++    P     E   KVE++P   GSA+A+PK       A GKK
Sbjct: 1   MGNICFRTNKVMAQDDSYDNLPPDQFIEAE-KVEEQPLRPGSAMAKPKNGTG--GAAGKK 60

Query: 61  VVRFQLREDEIS----GGGGGGGGGVLRIKVVVSQKELKQILKDRESNSSTLEELLAELK 120
           VVRF L+E+E        G   G GVLRIKVV+SQKELK+ILK+RE+NS +LEEL+ ELK
Sbjct: 61  VVRFNLQEEEEDQEDRNSGDDSGAGVLRIKVVISQKELKEILKNRENNSCSLEELIEELK 120

Query: 121 MKGRTISDARADKEEDENGSWRPALESIPE---DLH 148
           +KG      RA    DE GSW+PALE IPE   DLH
Sbjct: 121 VKG------RATTVSDEIGSWKPALECIPEGEGDLH 147

BLAST of MS004887.1 vs. ExPASy TrEMBL
Match: A0A6J1CG85 (uncharacterized protein LOC111011249 OS=Momordica charantia OX=3673 GN=LOC111011249 PE=4 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 1.3e-65
Identity = 139/147 (94.56%), Postives = 140/147 (95.24%), Query Frame = 0

Query: 1   MGNCLRSNRVMAQDEACSPSPNSSLTETNYKVEDKPAAGSALARPKTEEARIAARGKKVV 60
           MGNCLR+NRVMAQDEACSPSPNSSLTETNYKVEDKPAAGSALARPKTEEARIAAR KKVV
Sbjct: 43  MGNCLRNNRVMAQDEACSPSPNSSLTETNYKVEDKPAAGSALARPKTEEARIAARRKKVV 102

Query: 61  RFQLREDEISGGGGGGGGGVLRIKVVVSQKELKQILKDRESNSSTLEELLAELKMKGRTI 120
           RFQ REDEIS    GGGGGVLRIKVVVSQKELKQILKDRESNSSTLEELLAELKMKGRTI
Sbjct: 103 RFQQREDEIS----GGGGGVLRIKVVVSQKELKQILKDRESNSSTLEELLAELKMKGRTI 162

Query: 121 SDARADKEEDENGSWRPALESIPEDLH 148
           SDARAD EEDENGSWRPALESIPEDLH
Sbjct: 163 SDARADNEEDENGSWRPALESIPEDLH 185

BLAST of MS004887.1 vs. ExPASy TrEMBL
Match: A0A0A0LQE9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G418890 PE=4 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 1.8e-25
Identity = 81/148 (54.73%), Postives = 97/148 (65.54%), Query Frame = 0

Query: 1   MGN-CLRSNRVMAQDEACSPSPNSSLTETNYKVEDKPAAGSALARPKTEEARIAARGKKV 60
           MGN C +SN+VMAQD++    P   L E   KV+ +P  GSA+A+PK       A GKKV
Sbjct: 1   MGNICFKSNKVMAQDDSYDDFPPHHLIEPK-KVQQQPLPGSAMAKPKNGTG--GAAGKKV 60

Query: 61  VRFQLRE---DEISGGGGGGGGGVLRIKVVVSQKELKQILKDRESNSSTLEELLAELKMK 120
           VRF L+E   DE     G  G GVLRIKVV+SQKELKQILK RE+NS +LEEL+ ELK+K
Sbjct: 61  VRFNLQEEEKDEEGRNSGDSGPGVLRIKVVISQKELKQILKSRENNSCSLEELIEELKVK 120

Query: 121 GRTISDARADKEEDENGSWRPALESIPE 145
           GR  +        DE GSW+PALE IPE
Sbjct: 121 GRATT-----VSADETGSWKPALECIPE 140

BLAST of MS004887.1 vs. ExPASy TrEMBL
Match: A0A5D3DKZ8 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold27G00200 PE=4 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 2.5e-24
Identity = 83/156 (53.21%), Postives = 101/156 (64.74%), Query Frame = 0

Query: 1   MGN-CLRSNRVMAQDEACSPSPNSSLTETNYKVEDKP-AAGSALARPKTEEARIAARGKK 60
           MGN C R+N+VMAQD++    P     E   KVE++P   GSA+A+PK       A GKK
Sbjct: 1   MGNICFRTNKVMAQDDSYDNLPPDQFIEAE-KVEEQPLRPGSAMAKPKNGTG--GAAGKK 60

Query: 61  VVRFQLREDEIS----GGGGGGGGGVLRIKVVVSQKELKQILKDRESNSSTLEELLAELK 120
           VVRF L+E+E        G   G GVLRIKVV+SQKELK+ILK+RE+NS +LEEL+ ELK
Sbjct: 61  VVRFNLQEEEEDQEDRNSGDDSGAGVLRIKVVISQKELKEILKNRENNSCSLEELIEELK 120

Query: 121 MKGRTISDARADKEEDENGSWRPALESIPE---DLH 148
           +KG      RA    DE GSW+PALE IPE   DLH
Sbjct: 121 VKG------RATTVSDEIGSWKPALECIPEGEGDLH 147

BLAST of MS004887.1 vs. ExPASy TrEMBL
Match: A0A7N2MA05 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 2.4e-14
Identity = 66/150 (44.00%), Postives = 91/150 (60.67%), Query Frame = 0

Query: 1   MGNCLRSNRVMAQDEACSPSPNSSLTETNYKVEDKPAAGSALARPKTEEARIAARGKKVV 60
           MGNCL SN+ +AQ+E           E     + KP+  S L   K  +     + KKVV
Sbjct: 1   MGNCL-SNKSLAQEEEVP-------KEAEVVEQTKPSTASKLEPVKLVDGG-HKKKKKVV 60

Query: 61  RFQLREDEISGGGGGGG---GGVLRIKVVVSQKELKQILKDRES-NSSTLEELLAELKMK 120
           RF+L ED+ + G    G    GV+RI+VVV+QKELKQIL  +E    S++E+L+  L ++
Sbjct: 61  RFKLEEDDTNVGTSSEGDSRSGVVRIRVVVTQKELKQILDCKEGLKYSSVEQLVNALNLR 120

Query: 121 GRTISDAR-ADKEEDENGSWRPALESIPED 146
           GR IS+ R +D++E  N +WRPALESIPED
Sbjct: 121 GRKISEVRTSDEDEGINSNWRPALESIPED 141

BLAST of MS004887.1 vs. ExPASy TrEMBL
Match: A0A6J1B1M6 (uncharacterized protein LOC110423293 OS=Herrania umbratica OX=108875 GN=LOC110423293 PE=4 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 1.2e-13
Identity = 63/155 (40.65%), Postives = 92/155 (59.35%), Query Frame = 0

Query: 1   MGNCLRSNRVMAQDEACSPSPNSSLTETNYKVEDKPAAGSALARPKTEEARIAA------ 60
           MGNCL SN+++AQ++   P P     E    +E+       +   K E A +AA      
Sbjct: 1   MGNCLTSNKIVAQND--QPEPQGCRAEV---IEET----GKVTASKLERAEVAADEGEKV 60

Query: 61  RGKKVVRFQLREDEISGGG--GGGGGGVLRIKVVVSQKELKQILKDRES-NSSTLEELLA 120
           + KK+VRF+L E+    GG  G    GV+RI++VV+QKELKQIL  RE    ++LE L+ 
Sbjct: 61  KKKKMVRFKLNEENDVDGGRQGESKDGVVRIRLVVTQKELKQILSSREDLKHTSLEGLIR 120

Query: 121 ELKMKGRTISD-ARADKEEDENGSWRPALESIPED 146
            +K++G  IS+  R + ++  +G WRPALESIPE+
Sbjct: 121 VMKLRGVRISEGGRTNDDDGFHGGWRPALESIPEE 146

BLAST of MS004887.1 vs. TAIR 10
Match: AT3G21680.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-terminal protein myristoylation; LOCATED IN: cellular_component unknown; EXPRESSED IN: root, flower, stamen; EXPRESSED DURING: 4 anthesis, petal differentiation and expansion stage; Has 34 Blast hits to 34 proteins in 7 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 34; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 55.5 bits (132), Expect = 4.3e-08
Identity = 51/148 (34.46%), Postives = 70/148 (47.30%), Query Frame = 0

Query: 1   MGNCLRSNRVMAQDEA--CSPSPNSSLTETNYKVEDKPAAGSALARPKTEEARIAARGKK 60
           MGNCLR +  +A+ E     P P   L E           G    R + E  R      K
Sbjct: 1   MGNCLRHDNGVARKEKDDLDPEPLVKLLE----------EGKTSFRGEEESERSTEEESK 60

Query: 61  VVRFQLREDEISGGGGGGGGGVLRIKVVVSQKELKQILKDRESNSSTLEELLAELKMKGR 120
           VV                     RIKVVV++KEL+QIL   ++  +++++L+  LK  GR
Sbjct: 61  VV---------------------RIKVVVTKKELRQIL-GHKNGINSIQQLVHVLKDSGR 116

Query: 121 TISDARADKEEDENG--SWRPALESIPE 145
            IS A  +++E E G  +WRP LESIPE
Sbjct: 121 NISMASYEEDEKEEGDENWRPTLESIPE 116