Cp4.1LG19g00370.1 (mRNA) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG19g00370.1
Type	mRNA
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Eukaryotic translation initiation factor 3 subunit E
Location	Cp4.1LG19: 275180 .. 275598 (-)
Sequence length	309
RNA-Seq Expression	Cp4.1LG19g00370.1
Synteny	Cp4.1LG19g00370.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGATGGGTGGGAGCAAAAGAGGAAGAAAGGAGGTTTTACTCTAAGTTTGTAGTAAGATTGCCTTCAGCCAGTTAGTAACAGATCTGATTCTAAATCCTCTCTTTTAGTGCTGCTAAATAATGAGGTTCTCTAATGTATAAAGCTTGGTTCTCAGGATTTGGATTGGTAAAACAATACTTCCTGACCATTATTGTATATCTTCTGTCATTATAACTTGTGACTTTTCTTGGTTCATTTCGTTGTTATTATCTCAAGTGCTGGTAACTTGTGGCAGCGTCCTTGCTGAAAAGTTGAACTTGAATTATGAGGAAGCTGAGCGGTGGATTGTAAATCTCGTGCGCACGTCAAAGCTTGATGCAAAGCTTGATTCAAAGACAGGAACGGTTATAATGGAAACGAACGAGCCTGACGTGTAA

mRNA sequence

ATGGGATGGGTGGGAGCAAAAGAGGAAGAAAGGAGGTTTTACTCTAAGATTTGGATTGGTAAAACAATACTTCCTGACCATTATTGTATATCTTCTGTCATTATAACTTGTGACTTTTCTTGGTTCATTTCGTTGTTATTATCTCAAGTGCTGGTAACTTGTGGCAGCGTCCTTGCTGAAAAGTTGAACTTGAATTATGAGGAAGCTGAGCGGTGGATTGTAAATCTCGTGCGCACGTCAAAGCTTGATGCAAAGCTTGATTCAAAGACAGGAACGGTTATAATGGAAACGAACGAGCCTGACGTGTAA

Coding sequence (CDS)

ATGGGATGGGTGGGAGCAAAAGAGGAAGAAAGGAGGTTTTACTCTAAGATTTGGATTGGTAAAACAATACTTCCTGACCATTATTGTATATCTTCTGTCATTATAACTTGTGACTTTTCTTGGTTCATTTCGTTGTTATTATCTCAAGTGCTGGTAACTTGTGGCAGCGTCCTTGCTGAAAAGTTGAACTTGAATTATGAGGAAGCTGAGCGGTGGATTGTAAATCTCGTGCGCACGTCAAAGCTTGATGCAAAGCTTGATTCAAAGACAGGAACGGTTATAATGGAAACGAACGAGCCTGACGTGTAA

Protein sequence

MGWVGAKEEERRFYSKIWIGKTILPDHYCISSVIITCDFSWFISLLLSQVLVTCGSVLAEKLNLNYEEAERWIVNLVRTSKLDAKLDSKTGTVIMETNEPDV

Homology

[Close]

BLAST of Cp4.1LG19g00370.1 vs. ExPASy Swiss-Prot
Match: Q9C5Z3 (Eukaryotic translation initiation factor 3 subunit E OS=Arabidopsis thaliana OX=3702 GN=TIF3E1 PE=1 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 1.6e-14
Identity = 38/46 (82.61%), Postives = 44/46 (95.65%), Query Frame = 0


Query: 57  VLAEKLNLNYEEAERWIVNLVRTSKLDAKLDSKTGTVIMETNEPDV 103
           VLAEKLNLNYEEAERWIVNL+RTSKLDAK+DS++GTVIME  +P+V
Sbjct: 364 VLAEKLNLNYEEAERWIVNLIRTSKLDAKIDSESGTVIMEPTQPNV 409

[Close]

BLAST of Cp4.1LG19g00370.1 vs. ExPASy Swiss-Prot
Match: A7RWP6 (Eukaryotic translation initiation factor 3 subunit E OS=Nematostella vectensis OX=45351 GN=v1g163572 PE=3 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 9.1e-10
Identity = 31/47 (65.96%), Postives = 37/47 (78.72%), Query Frame = 0


Query: 56  SVLAEKLNLNYEEAERWIVNLVRTSKLDAKLDSKTGTVIMETNEPDV 103
           S+LAEKLN+  EEAERWIVNL+R +KLDAK+DSK G V+M    P V
Sbjct: 352 SMLAEKLNMTPEEAERWIVNLIRNAKLDAKIDSKLGHVVMGVQAPSV 398

[Close]

BLAST of Cp4.1LG19g00370.1 vs. ExPASy Swiss-Prot
Match: Q05AY2 (Eukaryotic translation initiation factor 3 subunit E-A OS=Xenopus laevis OX=8355 GN=eif3e-a PE=2 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 1.0e-08
Identity = 28/43 (65.12%), Postives = 36/43 (83.72%), Query Frame = 0


Query: 56  SVLAEKLNLNYEEAERWIVNLVRTSKLDAKLDSKTGTVIMETN 99
           S+LA+KLN+  EEAERWIVNL+R ++LDAK+DSK G V+M  N
Sbjct: 355 SMLADKLNMTPEEAERWIVNLIRNARLDAKIDSKLGHVVMGNN 397

[Close]

BLAST of Cp4.1LG19g00370.1 vs. ExPASy Swiss-Prot
Match: Q1LUA8 (Eukaryotic translation initiation factor 3 subunit E-B OS=Danio rerio OX=7955 GN=eif3eb PE=3 SV=2)

HSP 1 Score: 60.8 bits (146), Expect = 1.0e-08
Identity = 28/43 (65.12%), Postives = 36/43 (83.72%), Query Frame = 0


Query: 56  SVLAEKLNLNYEEAERWIVNLVRTSKLDAKLDSKTGTVIMETN 99
           S+LA+KLN+  EEAERWIVNL+R ++LDAK+DSK G V+M  N
Sbjct: 355 SMLADKLNMTPEEAERWIVNLIRNARLDAKIDSKLGHVVMGNN 397

[Close]

BLAST of Cp4.1LG19g00370.1 vs. ExPASy Swiss-Prot
Match: Q3B8M3 (Eukaryotic translation initiation factor 3 subunit E-B OS=Xenopus laevis OX=8355 GN=eif3e-b PE=2 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 1.0e-08
Identity = 28/43 (65.12%), Postives = 36/43 (83.72%), Query Frame = 0


Query: 56  SVLAEKLNLNYEEAERWIVNLVRTSKLDAKLDSKTGTVIMETN 99
           S+LA+KLN+  EEAERWIVNL+R ++LDAK+DSK G V+M  N
Sbjct: 355 SMLADKLNMTPEEAERWIVNLIRNARLDAKIDSKLGHVVMGNN 397

[Close]

BLAST of Cp4.1LG19g00370.1 vs. NCBI nr
Match: XP_023518937.1 (eukaryotic translation initiation factor 3 subunit E [Cucurbita pepo subsp. pepo])

HSP 1 Score: 93.2 bits (230), Expect = 1.15e-19
Identity = 47/47 (100.00%), Postives = 47/47 (100.00%), Query Frame = 0


Query: 56  SVLAEKLNLNYEEAERWIVNLVRTSKLDAKLDSKTGTVIMETNEPDV 102
           SVLAEKLNLNYEEAERWIVNLVRTSKLDAKLDSKTGTVIMETNEPDV
Sbjct: 359 SVLAEKLNLNYEEAERWIVNLVRTSKLDAKLDSKTGTVIMETNEPDV 405

[Close]

BLAST of Cp4.1LG19g00370.1 vs. NCBI nr
Match: KAG7027648.1 (Eukaryotic translation initiation factor 3 subunit E, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 92.4 bits (228), Expect = 2.19e-19
Identity = 46/47 (97.87%), Postives = 47/47 (100.00%), Query Frame = 0


Query: 56  SVLAEKLNLNYEEAERWIVNLVRTSKLDAKLDSKTGTVIMETNEPDV 102
           SVLAEKLNLNYEEAERWIVNLVRTSKLDAK+DSKTGTVIMETNEPDV
Sbjct: 359 SVLAEKLNLNYEEAERWIVNLVRTSKLDAKIDSKTGTVIMETNEPDV 405

[Close]

BLAST of Cp4.1LG19g00370.1 vs. NCBI nr
Match: XP_022966317.1 (eukaryotic translation initiation factor 3 subunit E [Cucurbita maxima])

HSP 1 Score: 92.4 bits (228), Expect = 2.19e-19
Identity = 46/47 (97.87%), Postives = 47/47 (100.00%), Query Frame = 0


Query: 56  SVLAEKLNLNYEEAERWIVNLVRTSKLDAKLDSKTGTVIMETNEPDV 102
           SVLAEKLNLNYEEAERWIVNLVRTSKLDAK+DSKTGTVIMETNEPDV
Sbjct: 359 SVLAEKLNLNYEEAERWIVNLVRTSKLDAKIDSKTGTVIMETNEPDV 405

[Close]

BLAST of Cp4.1LG19g00370.1 vs. NCBI nr
Match: KAG6595686.1 (Eukaryotic translation initiation factor 3 subunit E, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 92.4 bits (228), Expect = 2.84e-19
Identity = 46/47 (97.87%), Postives = 47/47 (100.00%), Query Frame = 0


Query: 56  SVLAEKLNLNYEEAERWIVNLVRTSKLDAKLDSKTGTVIMETNEPDV 102
           SVLAEKLNLNYEEAERWIVNLVRTSKLDAK+DSKTGTVIMETNEPDV
Sbjct: 359 SVLAEKLNLNYEEAERWIVNLVRTSKLDAKIDSKTGTVIMETNEPDV 405

[Close]

BLAST of Cp4.1LG19g00370.1 vs. NCBI nr
Match: XP_022925104.1 (eukaryotic translation initiation factor 3 subunit E-like [Cucurbita moschata])

HSP 1 Score: 89.4 bits (220), Expect = 2.84e-18
Identity = 44/47 (93.62%), Postives = 47/47 (100.00%), Query Frame = 0


Query: 56  SVLAEKLNLNYEEAERWIVNLVRTSKLDAKLDSKTGTVIMETNEPDV 102
           SVLAEKLNLNYEEAE+WIVNLVRTSKLDAK+DSKTGTVIMETN+PDV
Sbjct: 359 SVLAEKLNLNYEEAEQWIVNLVRTSKLDAKIDSKTGTVIMETNKPDV 405

[Close]

BLAST of Cp4.1LG19g00370.1 vs. ExPASy TrEMBL
Match: A0A6J1HMM9 (Eukaryotic translation initiation factor 3 subunit E OS=Cucurbita maxima OX=3661 GN=LOC111466010 PE=3 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 1.06e-19
Identity = 46/47 (97.87%), Postives = 47/47 (100.00%), Query Frame = 0


Query: 56  SVLAEKLNLNYEEAERWIVNLVRTSKLDAKLDSKTGTVIMETNEPDV 102
           SVLAEKLNLNYEEAERWIVNLVRTSKLDAK+DSKTGTVIMETNEPDV
Sbjct: 359 SVLAEKLNLNYEEAERWIVNLVRTSKLDAKIDSKTGTVIMETNEPDV 405

[Close]

BLAST of Cp4.1LG19g00370.1 vs. ExPASy TrEMBL
Match: A0A6J1EB55 (Eukaryotic translation initiation factor 3 subunit E OS=Cucurbita moschata OX=3662 GN=LOC111432445 PE=3 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 1.38e-18
Identity = 44/47 (93.62%), Postives = 47/47 (100.00%), Query Frame = 0


Query: 56  SVLAEKLNLNYEEAERWIVNLVRTSKLDAKLDSKTGTVIMETNEPDV 102
           SVLAEKLNLNYEEAE+WIVNLVRTSKLDAK+DSKTGTVIMETN+PDV
Sbjct: 359 SVLAEKLNLNYEEAEQWIVNLVRTSKLDAKIDSKTGTVIMETNKPDV 405

[Close]

BLAST of Cp4.1LG19g00370.1 vs. ExPASy TrEMBL
Match: A0A6J1EKJ5 (Eukaryotic translation initiation factor 3 subunit E OS=Cucurbita moschata OX=3662 GN=LOC111435512 PE=3 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 9.33e-18
Identity = 43/47 (91.49%), Postives = 46/47 (97.87%), Query Frame = 0


Query: 56  SVLAEKLNLNYEEAERWIVNLVRTSKLDAKLDSKTGTVIMETNEPDV 102
           SVLAEKLNLNYEEAERWIVNLVRTSKLDAK+DSKTGTVIME N+P+V
Sbjct: 359 SVLAEKLNLNYEEAERWIVNLVRTSKLDAKIDSKTGTVIMEPNQPNV 405

[Close]

BLAST of Cp4.1LG19g00370.1 vs. ExPASy TrEMBL
Match: A0A199UAE0 (PCI domain-containing protein OS=Manihot esculenta OX=3983 GN=MANES_S071900 PE=4 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 1.45e-17
Identity = 38/46 (82.61%), Postives = 44/46 (95.65%), Query Frame = 0


Query: 57  VLAEKLNLNYEEAERWIVNLVRTSKLDAKLDSKTGTVIMETNEPDV 102
           VLAEKLNLNYEEAERWIVNL+R SKLDAK+DS++GTVIME N+P+V
Sbjct: 3   VLAEKLNLNYEEAERWIVNLIRNSKLDAKIDSQSGTVIMEPNQPNV 48

[Close]

BLAST of Cp4.1LG19g00370.1 vs. ExPASy TrEMBL
Match: A0A2P5AQU8 (Eukaryotic translation initiation factor 3 subunit E OS=Trema orientale OX=63057 GN=TorRG33x02_343960 PE=4 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 4.50e-17
Identity = 40/46 (86.96%), Postives = 44/46 (95.65%), Query Frame = 0


Query: 57  VLAEKLNLNYEEAERWIVNLVRTSKLDAKLDSKTGTVIMETNEPDV 102
           VLAEKLNLNYEEAERWIVNL+RTSKLDAK+DSKTGTV+ME N P+V
Sbjct: 223 VLAEKLNLNYEEAERWIVNLIRTSKLDAKIDSKTGTVVMEPNHPNV 268

[Close]

BLAST of Cp4.1LG19g00370.1 vs. TAIR 10
Match: AT3G57290.1 (eukaryotic translation initiation factor 3E )

HSP 1 Score: 80.1 bits (196), Expect = 1.1e-15
Identity = 38/46 (82.61%), Postives = 44/46 (95.65%), Query Frame = 0


Query: 57  VLAEKLNLNYEEAERWIVNLVRTSKLDAKLDSKTGTVIMETNEPDV 103
           VLAEKLNLNYEEAERWIVNL+RTSKLDAK+DS++GTVIME  +P+V
Sbjct: 364 VLAEKLNLNYEEAERWIVNLIRTSKLDAKIDSESGTVIMEPTQPNV 409

The following BLAST results are available for this feature:

BLAST of Cp4.1LG19g00370.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C5Z3	1.6e-14	82.61	Eukaryotic translation initiation factor 3 subunit E OS=Arabidopsis thaliana OX=...	[more]
A7RWP6	9.1e-10	65.96	Eukaryotic translation initiation factor 3 subunit E OS=Nematostella vectensis O...	[more]
Q05AY2	1.0e-08	65.12	Eukaryotic translation initiation factor 3 subunit E-A OS=Xenopus laevis OX=8355...	[more]
Q1LUA8	1.0e-08	65.12	Eukaryotic translation initiation factor 3 subunit E-B OS=Danio rerio OX=7955 GN...	[more]
Q3B8M3	1.0e-08	65.12	Eukaryotic translation initiation factor 3 subunit E-B OS=Xenopus laevis OX=8355...	[more]

BLAST of Cp4.1LG19g00370.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023518937.1	1.15e-19	100.00	eukaryotic translation initiation factor 3 subunit E [Cucurbita pepo subsp. pepo...	[more]
KAG7027648.1	2.19e-19	97.87	Eukaryotic translation initiation factor 3 subunit E, partial [Cucurbita argyros...	[more]
XP_022966317.1	2.19e-19	97.87	eukaryotic translation initiation factor 3 subunit E [Cucurbita maxima]	[more]
KAG6595686.1	2.84e-19	97.87	Eukaryotic translation initiation factor 3 subunit E, partial [Cucurbita argyros...	[more]
XP_022925104.1	2.84e-18	93.62	eukaryotic translation initiation factor 3 subunit E-like [Cucurbita moschata]	[more]

BLAST of Cp4.1LG19g00370.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HMM9	1.06e-19	97.87	Eukaryotic translation initiation factor 3 subunit E OS=Cucurbita maxima OX=3661...	[more]
A0A6J1EB55	1.38e-18	93.62	Eukaryotic translation initiation factor 3 subunit E OS=Cucurbita moschata OX=36...	[more]
A0A6J1EKJ5	9.33e-18	91.49	Eukaryotic translation initiation factor 3 subunit E OS=Cucurbita moschata OX=36...	[more]
A0A199UAE0	1.45e-17	82.61	PCI domain-containing protein OS=Manihot esculenta OX=3983 GN=MANES_S071900 PE=4...	[more]
A0A2P5AQU8	4.50e-17	86.96	Eukaryotic translation initiation factor 3 subunit E OS=Trema orientale OX=63057...	[more]

BLAST of Cp4.1LG19g00370.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G57290.1	1.1e-15	82.61	eukaryotic translation initiation factor 3E	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000717	Proteasome component (PCI) domain	PFAM	PF01399	PCI	coord: 56..96 e-value: 1.3E-8 score: 35.3
IPR016650	Eukaryotic translation initiation factor 3 subunit E	PANTHER	PTHR10317	EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT E	coord: 57..101
None	No IPR available	PANTHER	PTHR10317:SF0	EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT E	coord: 57..101
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 56..100

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cp4.1LG19g00370	Cp4.1LG19g00370	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG19g00370.1:exon:001	Cp4.1LG19g00370.1:exon:001	exon
Cp4.1LG19g00370.1:exon:002	Cp4.1LG19g00370.1:exon:002	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG19g00370.1:cds:001	Cp4.1LG19g00370.1:cds:001	CDS
Cp4.1LG19g00370.1:cds:002	Cp4.1LG19g00370.1:cds:002	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cp4.1LG19g00370.1	Cp4.1LG19g00370.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001732	formation of cytoplasmic translation initiation complex
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005852	eukaryotic translation initiation factor 3 complex
cellular_component	GO:0016282	eukaryotic 43S preinitiation complex
cellular_component	GO:0033290	eukaryotic 48S preinitiation complex
cellular_component	GO:0071540	eukaryotic translation initiation factor 3 complex, eIF3e
molecular_function	GO:0003743	translation initiation factor activity