CmaCh14G008380.1 (mRNA) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh14G008380.1
Type	mRNA
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	calcium-binding protein CML24-like
Location	Cma_Chr14: 4303611 .. 4304075 (-)
Sequence length	465
RNA-Seq Expression	CmaCh14G008380.1
Synteny	CmaCh14G008380.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAAGAAGTCGGTTTCCAATCCAGGAGCCTTGGGCTCCATGGACGAGGTCATGAAGATCTTCAAGAAGTTCGACAAGAACGGCGACGGCAAGATTTCTGTTAGCGAGCTTGGCGCCGCCCTCGGTGAACTCGGCGGCAAAATCTCCTCGGACGAGACTCGCCGCATTATGTTAGAGATCGATACGGATGGCGACGGATTCATCGACCTTGACGAGTTTGCCGCGTTTCATCGGGAAGCCTATCCAGACGGCGGAGGTAACAAAGATCTACAGGATGCTTTCGATCTGTACGATATGGACAAGAACGGCCTAATCTCGGCCAAGGAGTTGCACTTTGTTCTCAAGCGCCTCGGTGAAAAATGTAGCCTCAAAGATTGTTGCCGGATGATCAGTTCCGTCGATGTCGACGGCGATGGCCATGTAAATTTCGAGGAGTTCAAGAAGATGATGTCGCGCTCTTAG

mRNA sequence

ATGGCGAAGAAGTCGGTTTCCAATCCAGGAGCCTTGGGCTCCATGGACGAGGTCATGAAGATCTTCAAGAAGTTCGACAAGAACGGCGACGGCAAGATTTCTGTTAGCGAGCTTGGCGCCGCCCTCGGTGAACTCGGCGGCAAAATCTCCTCGGACGAGACTCGCCGCATTATGTTAGAGATCGATACGGATGGCGACGGATTCATCGACCTTGACGAGTTTGCCGCGTTTCATCGGGAAGCCTATCCAGACGGCGGAGGTAACAAAGATCTACAGGATGCTTTCGATCTGTACGATATGGACAAGAACGGCCTAATCTCGGCCAAGGAGTTGCACTTTGTTCTCAAGCGCCTCGGTGAAAAATGTAGCCTCAAAGATTGTTGCCGGATGATCAGTTCCGTCGATGTCGACGGCGATGGCCATGTAAATTTCGAGGAGTTCAAGAAGATGATGTCGCGCTCTTAG

Coding sequence (CDS)

ATGGCGAAGAAGTCGGTTTCCAATCCAGGAGCCTTGGGCTCCATGGACGAGGTCATGAAGATCTTCAAGAAGTTCGACAAGAACGGCGACGGCAAGATTTCTGTTAGCGAGCTTGGCGCCGCCCTCGGTGAACTCGGCGGCAAAATCTCCTCGGACGAGACTCGCCGCATTATGTTAGAGATCGATACGGATGGCGACGGATTCATCGACCTTGACGAGTTTGCCGCGTTTCATCGGGAAGCCTATCCAGACGGCGGAGGTAACAAAGATCTACAGGATGCTTTCGATCTGTACGATATGGACAAGAACGGCCTAATCTCGGCCAAGGAGTTGCACTTTGTTCTCAAGCGCCTCGGTGAAAAATGTAGCCTCAAAGATTGTTGCCGGATGATCAGTTCCGTCGATGTCGACGGCGATGGCCATGTAAATTTCGAGGAGTTCAAGAAGATGATGTCGCGCTCTTAG

Protein sequence

MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLEIDTDGDGFIDLDEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS

Homology

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BLAST of CmaCh14G008380.1 vs. ExPASy Swiss-Prot
Match: P25070 (Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2)

HSP 1 Score: 173.3 bits (438), Expect = 2.1e-42
Identity = 88/156 (56.41%), Postives = 112/156 (71.79%), Query Frame = 0


Query: 1   MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLE 60
           M+ K+      LGSMD++ K+F++FDKNGDGKISV EL   +  L    S +ET  +M +
Sbjct: 1   MSSKNGVVRSCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQ 60

Query: 61  IDTDGDGFIDLDEFAAFHREAYPDGGGNK----DLQDAFDLYDMDKNGLISAKELHFVLK 120
            D DG+GFIDLDEF A  +     GG N+    DL++AF+LYD+D NG ISAKELH V+K
Sbjct: 61  FDLDGNGFIDLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMK 120

Query: 121 RLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMS 153
            LGEKCS++DC +MIS VD+DGDG VNF+EFKKMMS
Sbjct: 121 NLGEKCSVQDCKKMISKVDIDGDGCVNFDEFKKMMS 156

[Close]

BLAST of CmaCh14G008380.1 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 8.8e-41
Identity = 84/150 (56.00%), Postives = 110/150 (73.33%), Query Frame = 0


Query: 4   KSVSNPGALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLEIDT 63
           K+VS    LGSM+++ K+F++FDKN DGKIS+ EL   +G L    S +ET+ +M E D 
Sbjct: 3   KNVSR-NCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 62

Query: 64  DGDGFIDLDEFAAFHR--EAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEK 123
           DG+GFIDLDEF A  +  +   +    +DL++AFDLYD+D+NG ISA ELH V+K LGEK
Sbjct: 63  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 122

Query: 124 CSLKDCCRMISSVDVDGDGHVNFEEFKKMM 152
           CS++DC RMI+ VD DGDG V+FEEFKKMM
Sbjct: 123 CSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151

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BLAST of CmaCh14G008380.1 vs. ExPASy Swiss-Prot
Match: Q9M7R0 (Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 1.2e-37
Identity = 80/140 (57.14%), Postives = 98/140 (70.00%), Query Frame = 0


Query: 16  DEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLEIDTDGDGFIDLDEFA 75
           +EV  +F +FD NGDGKIS  EL   L  LG   S +E  RIM EIDTD DGFI++ EFA
Sbjct: 19  NEVQGVFNRFDANGDGKISGDELAGVLKALGSNTSKEEIGRIMEEIDTDKDGFINVQEFA 78

Query: 76  AF---HREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEKCSLKDCCRMIS 135
           AF     + YP  GG  +L++AF+LYD D NGLIS+ ELH +L RLGE+ +  DC  MI 
Sbjct: 79  AFVKAETDPYPSSGGENELKEAFELYDQDHNGLISSVELHKILTRLGERYAEHDCVEMIK 138

Query: 136 SVDVDGDGHVNFEEFKKMMS 153
           SVD DGDG+V+FEEFKKMM+
Sbjct: 139 SVDSDGDGYVSFEEFKKMMT 158

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BLAST of CmaCh14G008380.1 vs. ExPASy Swiss-Prot
Match: Q9LE22 (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 PE=1 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 1.2e-37
Identity = 77/143 (53.85%), Postives = 100/143 (69.93%), Query Frame = 0


Query: 12  LGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLEIDTDGDGFIDL 71
           + + +E+ K+F +FD NGDGKISV ELG     +G   +  E  R++ E+DTD DG+I+L
Sbjct: 18  MANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINL 77

Query: 72  DEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEKCSLKDCCRMI 131
           DEF+   R +        +++DAFDLYD DKNGLISA ELH VL RLG  CS++DC RMI
Sbjct: 78  DEFSTLCRSS----SSAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMI 137

Query: 132 SSVDVDGDGHVNFEEFKKMMSRS 155
             VD DGDG+VNFEEF+KMM+ S
Sbjct: 138 GPVDADGDGNVNFEEFQKMMTSS 156

[Close]

BLAST of CmaCh14G008380.1 vs. ExPASy Swiss-Prot
Match: Q0DJV6 (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 GN=CML18 PE=2 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 4.5e-37
Identity = 79/149 (53.02%), Postives = 101/149 (67.79%), Query Frame = 0


Query: 15  MDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLEIDTDGDGFIDLDEF 74
           M E+ ++F+++D NGDGKIS  EL + L  LG  +   E RR+M E+D+D DGF+DL EF
Sbjct: 3   MAELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEF 62

Query: 75  AAFHREAYPDGGG------------NKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEKC 134
           AAFH    P  GG              +L++AF +YD D NG ISA+ELH VL++LG+KC
Sbjct: 63  AAFHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDKC 122

Query: 135 SLKDCCRMISSVDVDGDGHVNFEEFKKMM 152
           S+ DC RMI SVD DGDG VNF+EFKKMM
Sbjct: 123 SVADCSRMIRSVDADGDGCVNFDEFKKMM 151

[Close]

BLAST of CmaCh14G008380.1 vs. ExPASy TrEMBL
Match: A0A6J1J811 (calcium-binding protein CML24-like OS=Cucurbita maxima OX=3661 GN=LOC111482109 PE=4 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 2.4e-81
Identity = 154/154 (100.00%), Postives = 154/154 (100.00%), Query Frame = 0


Query: 1   MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLE 60
           MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLE
Sbjct: 1   MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLE 60

Query: 61  IDTDGDGFIDLDEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE 120
           IDTDGDGFIDLDEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE
Sbjct: 61  IDTDGDGFIDLDEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE 120

Query: 121 KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 155
           KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS
Sbjct: 121 KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 154

[Close]

BLAST of CmaCh14G008380.1 vs. ExPASy TrEMBL
Match: A0A6J1F6R4 (calcium-binding protein CML24-like OS=Cucurbita moschata OX=3662 GN=LOC111441384 PE=4 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 1.9e-78
Identity = 147/154 (95.45%), Postives = 152/154 (98.70%), Query Frame = 0


Query: 1   MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLE 60
           MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKIS+SELGAALGELGG+ISSDE RRIMLE
Sbjct: 1   MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISISELGAALGELGGRISSDEIRRIMLE 60

Query: 61  IDTDGDGFIDLDEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE 120
           IDTDGDGFIDLDEF AFH++AYP+GGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE
Sbjct: 61  IDTDGDGFIDLDEFTAFHQDAYPEGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE 120

Query: 121 KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 155
           KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS
Sbjct: 121 KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 154

[Close]

BLAST of CmaCh14G008380.1 vs. ExPASy TrEMBL
Match: A0A6J1CNU4 (probable calcium-binding protein CML23 OS=Momordica charantia OX=3673 GN=LOC111013180 PE=4 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 5.1e-68
Identity = 134/160 (83.75%), Postives = 142/160 (88.75%), Query Frame = 0


Query: 1   MAKKSVSNP------GALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDET 60
           MAKKSVS+P      GALGSMDEV K+F KFDKNGDGKISV+ELG ALGELGGK+SSDE 
Sbjct: 3   MAKKSVSDPSSTNLGGALGSMDEVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEV 62

Query: 61  RRIMLEIDTDGDGFIDLDEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFV 120
           RRIM EID DGDGFIDLDEFA FH++    GGGNKDLQDAFDLYD+DKNGLISAKELH V
Sbjct: 63  RRIMAEIDKDGDGFIDLDEFAEFHQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSV 122

Query: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 155
           LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMM+RS
Sbjct: 123 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 162

[Close]

BLAST of CmaCh14G008380.1 vs. ExPASy TrEMBL
Match: A0A6J1G9H5 (probable calcium-binding protein CML23 OS=Cucurbita moschata OX=3662 GN=LOC111452166 PE=4 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 6.3e-66
Identity = 131/160 (81.88%), Postives = 140/160 (87.50%), Query Frame = 0


Query: 1   MAKKSVSNP------GALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDET 60
           MAK SVSNP      GALGSMDEV+ +FKKFDKNGDGKIS+ EL AALGELGGKIS DE 
Sbjct: 3   MAKTSVSNPSSPNPGGALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKISDDEI 62

Query: 61  RRIMLEIDTDGDGFIDLDEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFV 120
           RRIM EID DG+GFIDLDEFA FH+ +   GGG+KDLQDAFDLYD+DKNGLISAKELH V
Sbjct: 63  RRIMSEIDKDGNGFIDLDEFAEFHQNSSSTGGGSKDLQDAFDLYDVDKNGLISAKELHSV 122

Query: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 155
           LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMM+RS
Sbjct: 123 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 162

[Close]

BLAST of CmaCh14G008380.1 vs. ExPASy TrEMBL
Match: A0A6J1KB05 (probable calcium-binding protein CML23 OS=Cucurbita maxima OX=3661 GN=LOC111493297 PE=4 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 4.1e-65
Identity = 130/160 (81.25%), Postives = 139/160 (86.88%), Query Frame = 0


Query: 1   MAKKSVSNP------GALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDET 60
           MAK SVSNP      GALGSMDEV+ +FKKFDKNGDGKIS+ EL AALGELGGKIS DE 
Sbjct: 3   MAKTSVSNPSSPNPGGALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKISDDEI 62

Query: 61  RRIMLEIDTDGDGFIDLDEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFV 120
           RRIM EID DG+GFIDLDEFA FH+     GGG+KDLQDAFDL+D+DKNGLISAKELH V
Sbjct: 63  RRIMSEIDKDGNGFIDLDEFAEFHQNFSSTGGGSKDLQDAFDLFDVDKNGLISAKELHSV 122

Query: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 155
           LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMM+RS
Sbjct: 123 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 162

[Close]

BLAST of CmaCh14G008380.1 vs. NCBI nr
Match: XP_022983538.1 (calcium-binding protein CML24-like [Cucurbita maxima])

HSP 1 Score: 311.2 bits (796), Expect = 4.9e-81
Identity = 154/154 (100.00%), Postives = 154/154 (100.00%), Query Frame = 0


Query: 1   MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLE 60
           MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLE
Sbjct: 1   MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLE 60

Query: 61  IDTDGDGFIDLDEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE 120
           IDTDGDGFIDLDEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE
Sbjct: 61  IDTDGDGFIDLDEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE 120

Query: 121 KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 155
           KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS
Sbjct: 121 KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 154

[Close]

BLAST of CmaCh14G008380.1 vs. NCBI nr
Match: XP_023526443.1 (calcium-binding protein CML24-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 302.8 bits (774), Expect = 1.7e-78
Identity = 148/154 (96.10%), Postives = 152/154 (98.70%), Query Frame = 0


Query: 1   MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLE 60
           MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKIS+SELGAALGELGGKISSDE RRIMLE
Sbjct: 1   MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISISELGAALGELGGKISSDEIRRIMLE 60

Query: 61  IDTDGDGFIDLDEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE 120
           IDTDGDGFIDLDEF AFH++AYP+GGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE
Sbjct: 61  IDTDGDGFIDLDEFTAFHQDAYPEGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE 120

Query: 121 KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 155
           KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS
Sbjct: 121 KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 154

[Close]

BLAST of CmaCh14G008380.1 vs. NCBI nr
Match: XP_022934113.1 (calcium-binding protein CML24-like [Cucurbita moschata] >KAG6581203.1 putative calcium-binding protein CML23, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017942.1 putative calcium-binding protein CML23, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 301.6 bits (771), Expect = 3.9e-78
Identity = 147/154 (95.45%), Postives = 152/154 (98.70%), Query Frame = 0


Query: 1   MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLE 60
           MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKIS+SELGAALGELGG+ISSDE RRIMLE
Sbjct: 1   MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISISELGAALGELGGRISSDEIRRIMLE 60

Query: 61  IDTDGDGFIDLDEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE 120
           IDTDGDGFIDLDEF AFH++AYP+GGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE
Sbjct: 61  IDTDGDGFIDLDEFTAFHQDAYPEGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE 120

Query: 121 KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 155
           KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS
Sbjct: 121 KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 154

[Close]

BLAST of CmaCh14G008380.1 vs. NCBI nr
Match: XP_022143269.1 (probable calcium-binding protein CML23 [Momordica charantia])

HSP 1 Score: 266.9 bits (681), Expect = 1.1e-67
Identity = 134/160 (83.75%), Postives = 142/160 (88.75%), Query Frame = 0


Query: 1   MAKKSVSNP------GALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDET 60
           MAKKSVS+P      GALGSMDEV K+F KFDKNGDGKISV+ELG ALGELGGK+SSDE 
Sbjct: 3   MAKKSVSDPSSTNLGGALGSMDEVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEV 62

Query: 61  RRIMLEIDTDGDGFIDLDEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFV 120
           RRIM EID DGDGFIDLDEFA FH++    GGGNKDLQDAFDLYD+DKNGLISAKELH V
Sbjct: 63  RRIMAEIDKDGDGFIDLDEFAEFHQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSV 122

Query: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 155
           LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMM+RS
Sbjct: 123 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 162

[Close]

BLAST of CmaCh14G008380.1 vs. NCBI nr
Match: XP_022948508.1 (probable calcium-binding protein CML23 [Cucurbita moschata] >XP_023524703.1 probable calcium-binding protein CML23 [Cucurbita pepo subsp. pepo] >KAG6607012.1 putative calcium-binding protein CML23, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036709.1 putative calcium-binding protein CML23, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 260.0 bits (663), Expect = 1.3e-65
Identity = 131/160 (81.88%), Postives = 140/160 (87.50%), Query Frame = 0


Query: 1   MAKKSVSNP------GALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDET 60
           MAK SVSNP      GALGSMDEV+ +FKKFDKNGDGKIS+ EL AALGELGGKIS DE 
Sbjct: 3   MAKTSVSNPSSPNPGGALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKISDDEI 62

Query: 61  RRIMLEIDTDGDGFIDLDEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFV 120
           RRIM EID DG+GFIDLDEFA FH+ +   GGG+KDLQDAFDLYD+DKNGLISAKELH V
Sbjct: 63  RRIMSEIDKDGNGFIDLDEFAEFHQNSSSTGGGSKDLQDAFDLYDVDKNGLISAKELHSV 122

Query: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 155
           LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMM+RS
Sbjct: 123 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 162

[Close]

BLAST of CmaCh14G008380.1 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 173.3 bits (438), Expect = 1.5e-43
Identity = 88/156 (56.41%), Postives = 112/156 (71.79%), Query Frame = 0


Query: 1   MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLE 60
           M+ K+      LGSMD++ K+F++FDKNGDGKISV EL   +  L    S +ET  +M +
Sbjct: 1   MSSKNGVVRSCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQ 60

Query: 61  IDTDGDGFIDLDEFAAFHREAYPDGGGNK----DLQDAFDLYDMDKNGLISAKELHFVLK 120
            D DG+GFIDLDEF A  +     GG N+    DL++AF+LYD+D NG ISAKELH V+K
Sbjct: 61  FDLDGNGFIDLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMK 120

Query: 121 RLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMS 153
            LGEKCS++DC +MIS VD+DGDG VNF+EFKKMMS
Sbjct: 121 NLGEKCSVQDCKKMISKVDIDGDGCVNFDEFKKMMS 156

[Close]

BLAST of CmaCh14G008380.1 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 167.9 bits (424), Expect = 6.2e-42
Identity = 84/150 (56.00%), Postives = 110/150 (73.33%), Query Frame = 0


Query: 4   KSVSNPGALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLEIDT 63
           K+VS    LGSM+++ K+F++FDKN DGKIS+ EL   +G L    S +ET+ +M E D 
Sbjct: 3   KNVSR-NCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 62

Query: 64  DGDGFIDLDEFAAFHR--EAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEK 123
           DG+GFIDLDEF A  +  +   +    +DL++AFDLYD+D+NG ISA ELH V+K LGEK
Sbjct: 63  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 122

Query: 124 CSLKDCCRMISSVDVDGDGHVNFEEFKKMM 152
           CS++DC RMI+ VD DGDG V+FEEFKKMM
Sbjct: 123 CSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151

[Close]

BLAST of CmaCh14G008380.1 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 157.5 bits (397), Expect = 8.4e-39
Identity = 77/143 (53.85%), Postives = 100/143 (69.93%), Query Frame = 0


Query: 12  LGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLEIDTDGDGFIDL 71
           + + +E+ K+F +FD NGDGKISV ELG     +G   +  E  R++ E+DTD DG+I+L
Sbjct: 18  MANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINL 77

Query: 72  DEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEKCSLKDCCRMI 131
           DEF+   R +        +++DAFDLYD DKNGLISA ELH VL RLG  CS++DC RMI
Sbjct: 78  DEFSTLCRSS----SSAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMI 137

Query: 132 SSVDVDGDGHVNFEEFKKMMSRS 155
             VD DGDG+VNFEEF+KMM+ S
Sbjct: 138 GPVDADGDGNVNFEEFQKMMTSS 156

[Close]

BLAST of CmaCh14G008380.1 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 157.5 bits (397), Expect = 8.4e-39
Identity = 77/143 (53.85%), Postives = 100/143 (69.93%), Query Frame = 0


Query: 12  LGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLEIDTDGDGFIDL 71
           + + +E+ K+F +FD NGDGKISV ELG     +G   +  E  R++ E+DTD DG+I+L
Sbjct: 18  MANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINL 77

Query: 72  DEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEKCSLKDCCRMI 131
           DEF+   R +        +++DAFDLYD DKNGLISA ELH VL RLG  CS++DC RMI
Sbjct: 78  DEFSTLCRSS----SSAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMI 137

Query: 132 SSVDVDGDGHVNFEEFKKMMSRS 155
             VD DGDG+VNFEEF+KMM+ S
Sbjct: 138 GPVDADGDGNVNFEEFQKMMTSS 156

[Close]

BLAST of CmaCh14G008380.1 vs. TAIR 10
Match: AT1G73630.1 (EF hand calcium-binding protein family )

HSP 1 Score: 150.2 bits (378), Expect = 1.3e-36
Identity = 75/136 (55.15%), Postives = 96/136 (70.59%), Query Frame = 0


Query: 17  EVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLEIDTDGDGFIDLDEFAA 76
           E+ K+F KFD NGDGKISVSELG     +G   + +E  R++ EID D DGFI+ +EFA 
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEFAT 79

Query: 77  FHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEKCSLKDCCRMISSVDV 136
             R +        ++++AFDLYD +KNGLIS+ E+H VL RLG  CS++DC RMI  VD 
Sbjct: 80  ICRSS----SSAVEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVDT 139

Query: 137 DGDGHVNFEEFKKMMS 153
           DGDG+VNFEEF+KMMS
Sbjct: 140 DGDGNVNFEEFQKMMS 151

The following BLAST results are available for this feature:

BLAST of CmaCh14G008380.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P25070	2.1e-42	56.41	Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2	[more]
Q9C8Y1	8.8e-41	56.00	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ...	[more]
Q9M7R0	1.2e-37	57.14	Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1	[more]
Q9LE22	1.2e-37	53.85	Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 ...	[more]
Q0DJV6	4.5e-37	53.02	Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of CmaCh14G008380.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J811	2.4e-81	100.00	calcium-binding protein CML24-like OS=Cucurbita maxima OX=3661 GN=LOC111482109 P...	[more]
A0A6J1F6R4	1.9e-78	95.45	calcium-binding protein CML24-like OS=Cucurbita moschata OX=3662 GN=LOC111441384...	[more]
A0A6J1CNU4	5.1e-68	83.75	probable calcium-binding protein CML23 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1G9H5	6.3e-66	81.88	probable calcium-binding protein CML23 OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]
A0A6J1KB05	4.1e-65	81.25	probable calcium-binding protein CML23 OS=Cucurbita maxima OX=3661 GN=LOC1114932...	[more]

BLAST of CmaCh14G008380.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022983538.1	4.9e-81	100.00	calcium-binding protein CML24-like [Cucurbita maxima]	[more]
XP_023526443.1	1.7e-78	96.10	calcium-binding protein CML24-like [Cucurbita pepo subsp. pepo]	[more]
XP_022934113.1	3.9e-78	95.45	calcium-binding protein CML24-like [Cucurbita moschata] >KAG6581203.1 putative c...	[more]
XP_022143269.1	1.1e-67	83.75	probable calcium-binding protein CML23 [Momordica charantia]	[more]
XP_022948508.1	1.3e-65	81.88	probable calcium-binding protein CML23 [Cucurbita moschata] >XP_023524703.1 prob...	[more]

BLAST of CmaCh14G008380.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G37770.1	1.5e-43	56.41	EF hand calcium-binding protein family	[more]
AT1G66400.1	6.2e-42	56.00	calmodulin like 23	[more]
AT1G18210.1	8.4e-39	53.85	Calcium-binding EF-hand family protein	[more]
AT1G18210.2	8.4e-39	53.85	Calcium-binding EF-hand family protein	[more]
AT1G73630.1	1.3e-36	55.15	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 126..154 e-value: 2.4E-4 score: 30.5 coord: 90..118 e-value: 1.2E-4 score: 31.5 coord: 53..81 e-value: 8.7E-4 score: 28.6 coord: 17..45 e-value: 2.7E-7 score: 40.2
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 17..77 e-value: 4.9E-14 score: 52.5 coord: 89..152 e-value: 2.7E-10 score: 40.6
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 13..48 score: 16.521873
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 49..84 score: 12.923368
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 86..121 score: 13.676544
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 122..154 score: 13.062845
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 17..79 e-value: 8.4636E-18 score: 70.6545
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 90..152 e-value: 4.23889E-18 score: 71.4249
None	No IPR available	GENE3D	1.10.238.10		coord: 89..154 e-value: 1.1E-21 score: 78.9
None	No IPR available	GENE3D	1.10.238.10		coord: 2..88 e-value: 2.5E-21 score: 78.5
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 7..152
None	No IPR available	PANTHER	PTHR10891:SF794	CALCIUM-BINDING PROTEIN CML23-RELATED	coord: 7..152
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 26..38
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 135..147
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 99..111
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 62..74
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 15..152

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmaCh14G008380	CmaCh14G008380	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh14G008380.1:exon:699	CmaCh14G008380.1:exon:699	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh14G008380.1:cds	CmaCh14G008380.1:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmaCh14G008380.1	CmaCh14G008380.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding