CmaCh14G008380 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh14G008380
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptioncalcium-binding protein CML24-like
LocationCma_Chr14: 4303611 .. 4304075 (-)
RNA-Seq ExpressionCmaCh14G008380
SyntenyCmaCh14G008380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAAGAAGTCGGTTTCCAATCCAGGAGCCTTGGGCTCCATGGACGAGGTCATGAAGATCTTCAAGAAGTTCGACAAGAACGGCGACGGCAAGATTTCTGTTAGCGAGCTTGGCGCCGCCCTCGGTGAACTCGGCGGCAAAATCTCCTCGGACGAGACTCGCCGCATTATGTTAGAGATCGATACGGATGGCGACGGATTCATCGACCTTGACGAGTTTGCCGCGTTTCATCGGGAAGCCTATCCAGACGGCGGAGGTAACAAAGATCTACAGGATGCTTTCGATCTGTACGATATGGACAAGAACGGCCTAATCTCGGCCAAGGAGTTGCACTTTGTTCTCAAGCGCCTCGGTGAAAAATGTAGCCTCAAAGATTGTTGCCGGATGATCAGTTCCGTCGATGTCGACGGCGATGGCCATGTAAATTTCGAGGAGTTCAAGAAGATGATGTCGCGCTCTTAG

mRNA sequence

ATGGCGAAGAAGTCGGTTTCCAATCCAGGAGCCTTGGGCTCCATGGACGAGGTCATGAAGATCTTCAAGAAGTTCGACAAGAACGGCGACGGCAAGATTTCTGTTAGCGAGCTTGGCGCCGCCCTCGGTGAACTCGGCGGCAAAATCTCCTCGGACGAGACTCGCCGCATTATGTTAGAGATCGATACGGATGGCGACGGATTCATCGACCTTGACGAGTTTGCCGCGTTTCATCGGGAAGCCTATCCAGACGGCGGAGGTAACAAAGATCTACAGGATGCTTTCGATCTGTACGATATGGACAAGAACGGCCTAATCTCGGCCAAGGAGTTGCACTTTGTTCTCAAGCGCCTCGGTGAAAAATGTAGCCTCAAAGATTGTTGCCGGATGATCAGTTCCGTCGATGTCGACGGCGATGGCCATGTAAATTTCGAGGAGTTCAAGAAGATGATGTCGCGCTCTTAG

Coding sequence (CDS)

ATGGCGAAGAAGTCGGTTTCCAATCCAGGAGCCTTGGGCTCCATGGACGAGGTCATGAAGATCTTCAAGAAGTTCGACAAGAACGGCGACGGCAAGATTTCTGTTAGCGAGCTTGGCGCCGCCCTCGGTGAACTCGGCGGCAAAATCTCCTCGGACGAGACTCGCCGCATTATGTTAGAGATCGATACGGATGGCGACGGATTCATCGACCTTGACGAGTTTGCCGCGTTTCATCGGGAAGCCTATCCAGACGGCGGAGGTAACAAAGATCTACAGGATGCTTTCGATCTGTACGATATGGACAAGAACGGCCTAATCTCGGCCAAGGAGTTGCACTTTGTTCTCAAGCGCCTCGGTGAAAAATGTAGCCTCAAAGATTGTTGCCGGATGATCAGTTCCGTCGATGTCGACGGCGATGGCCATGTAAATTTCGAGGAGTTCAAGAAGATGATGTCGCGCTCTTAG

Protein sequence

MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLEIDTDGDGFIDLDEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS
Homology
BLAST of CmaCh14G008380 vs. ExPASy Swiss-Prot
Match: P25070 (Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2)

HSP 1 Score: 173.3 bits (438), Expect = 2.1e-42
Identity = 88/156 (56.41%), Postives = 112/156 (71.79%), Query Frame = 0

Query: 1   MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLE 60
           M+ K+      LGSMD++ K+F++FDKNGDGKISV EL   +  L    S +ET  +M +
Sbjct: 1   MSSKNGVVRSCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQ 60

Query: 61  IDTDGDGFIDLDEFAAFHREAYPDGGGNK----DLQDAFDLYDMDKNGLISAKELHFVLK 120
            D DG+GFIDLDEF A  +     GG N+    DL++AF+LYD+D NG ISAKELH V+K
Sbjct: 61  FDLDGNGFIDLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMK 120

Query: 121 RLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMS 153
            LGEKCS++DC +MIS VD+DGDG VNF+EFKKMMS
Sbjct: 121 NLGEKCSVQDCKKMISKVDIDGDGCVNFDEFKKMMS 156

BLAST of CmaCh14G008380 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 8.8e-41
Identity = 84/150 (56.00%), Postives = 110/150 (73.33%), Query Frame = 0

Query: 4   KSVSNPGALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLEIDT 63
           K+VS    LGSM+++ K+F++FDKN DGKIS+ EL   +G L    S +ET+ +M E D 
Sbjct: 3   KNVSR-NCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 62

Query: 64  DGDGFIDLDEFAAFHR--EAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEK 123
           DG+GFIDLDEF A  +  +   +    +DL++AFDLYD+D+NG ISA ELH V+K LGEK
Sbjct: 63  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 122

Query: 124 CSLKDCCRMISSVDVDGDGHVNFEEFKKMM 152
           CS++DC RMI+ VD DGDG V+FEEFKKMM
Sbjct: 123 CSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151

BLAST of CmaCh14G008380 vs. ExPASy Swiss-Prot
Match: Q9M7R0 (Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 1.2e-37
Identity = 80/140 (57.14%), Postives = 98/140 (70.00%), Query Frame = 0

Query: 16  DEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLEIDTDGDGFIDLDEFA 75
           +EV  +F +FD NGDGKIS  EL   L  LG   S +E  RIM EIDTD DGFI++ EFA
Sbjct: 19  NEVQGVFNRFDANGDGKISGDELAGVLKALGSNTSKEEIGRIMEEIDTDKDGFINVQEFA 78

Query: 76  AF---HREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEKCSLKDCCRMIS 135
           AF     + YP  GG  +L++AF+LYD D NGLIS+ ELH +L RLGE+ +  DC  MI 
Sbjct: 79  AFVKAETDPYPSSGGENELKEAFELYDQDHNGLISSVELHKILTRLGERYAEHDCVEMIK 138

Query: 136 SVDVDGDGHVNFEEFKKMMS 153
           SVD DGDG+V+FEEFKKMM+
Sbjct: 139 SVDSDGDGYVSFEEFKKMMT 158

BLAST of CmaCh14G008380 vs. ExPASy Swiss-Prot
Match: Q9LE22 (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 PE=1 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 1.2e-37
Identity = 77/143 (53.85%), Postives = 100/143 (69.93%), Query Frame = 0

Query: 12  LGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLEIDTDGDGFIDL 71
           + + +E+ K+F +FD NGDGKISV ELG     +G   +  E  R++ E+DTD DG+I+L
Sbjct: 18  MANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINL 77

Query: 72  DEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEKCSLKDCCRMI 131
           DEF+   R +        +++DAFDLYD DKNGLISA ELH VL RLG  CS++DC RMI
Sbjct: 78  DEFSTLCRSS----SSAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMI 137

Query: 132 SSVDVDGDGHVNFEEFKKMMSRS 155
             VD DGDG+VNFEEF+KMM+ S
Sbjct: 138 GPVDADGDGNVNFEEFQKMMTSS 156

BLAST of CmaCh14G008380 vs. ExPASy Swiss-Prot
Match: Q0DJV6 (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 GN=CML18 PE=2 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 4.5e-37
Identity = 79/149 (53.02%), Postives = 101/149 (67.79%), Query Frame = 0

Query: 15  MDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLEIDTDGDGFIDLDEF 74
           M E+ ++F+++D NGDGKIS  EL + L  LG  +   E RR+M E+D+D DGF+DL EF
Sbjct: 3   MAELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEF 62

Query: 75  AAFHREAYPDGGG------------NKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEKC 134
           AAFH    P  GG              +L++AF +YD D NG ISA+ELH VL++LG+KC
Sbjct: 63  AAFHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDKC 122

Query: 135 SLKDCCRMISSVDVDGDGHVNFEEFKKMM 152
           S+ DC RMI SVD DGDG VNF+EFKKMM
Sbjct: 123 SVADCSRMIRSVDADGDGCVNFDEFKKMM 151

BLAST of CmaCh14G008380 vs. ExPASy TrEMBL
Match: A0A6J1J811 (calcium-binding protein CML24-like OS=Cucurbita maxima OX=3661 GN=LOC111482109 PE=4 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 2.4e-81
Identity = 154/154 (100.00%), Postives = 154/154 (100.00%), Query Frame = 0

Query: 1   MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLE 60
           MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLE
Sbjct: 1   MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLE 60

Query: 61  IDTDGDGFIDLDEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE 120
           IDTDGDGFIDLDEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE
Sbjct: 61  IDTDGDGFIDLDEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE 120

Query: 121 KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 155
           KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS
Sbjct: 121 KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 154

BLAST of CmaCh14G008380 vs. ExPASy TrEMBL
Match: A0A6J1F6R4 (calcium-binding protein CML24-like OS=Cucurbita moschata OX=3662 GN=LOC111441384 PE=4 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 1.9e-78
Identity = 147/154 (95.45%), Postives = 152/154 (98.70%), Query Frame = 0

Query: 1   MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLE 60
           MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKIS+SELGAALGELGG+ISSDE RRIMLE
Sbjct: 1   MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISISELGAALGELGGRISSDEIRRIMLE 60

Query: 61  IDTDGDGFIDLDEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE 120
           IDTDGDGFIDLDEF AFH++AYP+GGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE
Sbjct: 61  IDTDGDGFIDLDEFTAFHQDAYPEGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE 120

Query: 121 KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 155
           KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS
Sbjct: 121 KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 154

BLAST of CmaCh14G008380 vs. ExPASy TrEMBL
Match: A0A6J1CNU4 (probable calcium-binding protein CML23 OS=Momordica charantia OX=3673 GN=LOC111013180 PE=4 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 5.1e-68
Identity = 134/160 (83.75%), Postives = 142/160 (88.75%), Query Frame = 0

Query: 1   MAKKSVSNP------GALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDET 60
           MAKKSVS+P      GALGSMDEV K+F KFDKNGDGKISV+ELG ALGELGGK+SSDE 
Sbjct: 3   MAKKSVSDPSSTNLGGALGSMDEVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEV 62

Query: 61  RRIMLEIDTDGDGFIDLDEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFV 120
           RRIM EID DGDGFIDLDEFA FH++    GGGNKDLQDAFDLYD+DKNGLISAKELH V
Sbjct: 63  RRIMAEIDKDGDGFIDLDEFAEFHQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSV 122

Query: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 155
           LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMM+RS
Sbjct: 123 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 162

BLAST of CmaCh14G008380 vs. ExPASy TrEMBL
Match: A0A6J1G9H5 (probable calcium-binding protein CML23 OS=Cucurbita moschata OX=3662 GN=LOC111452166 PE=4 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 6.3e-66
Identity = 131/160 (81.88%), Postives = 140/160 (87.50%), Query Frame = 0

Query: 1   MAKKSVSNP------GALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDET 60
           MAK SVSNP      GALGSMDEV+ +FKKFDKNGDGKIS+ EL AALGELGGKIS DE 
Sbjct: 3   MAKTSVSNPSSPNPGGALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKISDDEI 62

Query: 61  RRIMLEIDTDGDGFIDLDEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFV 120
           RRIM EID DG+GFIDLDEFA FH+ +   GGG+KDLQDAFDLYD+DKNGLISAKELH V
Sbjct: 63  RRIMSEIDKDGNGFIDLDEFAEFHQNSSSTGGGSKDLQDAFDLYDVDKNGLISAKELHSV 122

Query: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 155
           LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMM+RS
Sbjct: 123 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 162

BLAST of CmaCh14G008380 vs. ExPASy TrEMBL
Match: A0A6J1KB05 (probable calcium-binding protein CML23 OS=Cucurbita maxima OX=3661 GN=LOC111493297 PE=4 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 4.1e-65
Identity = 130/160 (81.25%), Postives = 139/160 (86.88%), Query Frame = 0

Query: 1   MAKKSVSNP------GALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDET 60
           MAK SVSNP      GALGSMDEV+ +FKKFDKNGDGKIS+ EL AALGELGGKIS DE 
Sbjct: 3   MAKTSVSNPSSPNPGGALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKISDDEI 62

Query: 61  RRIMLEIDTDGDGFIDLDEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFV 120
           RRIM EID DG+GFIDLDEFA FH+     GGG+KDLQDAFDL+D+DKNGLISAKELH V
Sbjct: 63  RRIMSEIDKDGNGFIDLDEFAEFHQNFSSTGGGSKDLQDAFDLFDVDKNGLISAKELHSV 122

Query: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 155
           LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMM+RS
Sbjct: 123 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 162

BLAST of CmaCh14G008380 vs. NCBI nr
Match: XP_022983538.1 (calcium-binding protein CML24-like [Cucurbita maxima])

HSP 1 Score: 311.2 bits (796), Expect = 4.9e-81
Identity = 154/154 (100.00%), Postives = 154/154 (100.00%), Query Frame = 0

Query: 1   MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLE 60
           MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLE
Sbjct: 1   MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLE 60

Query: 61  IDTDGDGFIDLDEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE 120
           IDTDGDGFIDLDEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE
Sbjct: 61  IDTDGDGFIDLDEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE 120

Query: 121 KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 155
           KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS
Sbjct: 121 KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 154

BLAST of CmaCh14G008380 vs. NCBI nr
Match: XP_023526443.1 (calcium-binding protein CML24-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 302.8 bits (774), Expect = 1.7e-78
Identity = 148/154 (96.10%), Postives = 152/154 (98.70%), Query Frame = 0

Query: 1   MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLE 60
           MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKIS+SELGAALGELGGKISSDE RRIMLE
Sbjct: 1   MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISISELGAALGELGGKISSDEIRRIMLE 60

Query: 61  IDTDGDGFIDLDEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE 120
           IDTDGDGFIDLDEF AFH++AYP+GGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE
Sbjct: 61  IDTDGDGFIDLDEFTAFHQDAYPEGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE 120

Query: 121 KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 155
           KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS
Sbjct: 121 KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 154

BLAST of CmaCh14G008380 vs. NCBI nr
Match: XP_022934113.1 (calcium-binding protein CML24-like [Cucurbita moschata] >KAG6581203.1 putative calcium-binding protein CML23, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017942.1 putative calcium-binding protein CML23, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 301.6 bits (771), Expect = 3.9e-78
Identity = 147/154 (95.45%), Postives = 152/154 (98.70%), Query Frame = 0

Query: 1   MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLE 60
           MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKIS+SELGAALGELGG+ISSDE RRIMLE
Sbjct: 1   MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISISELGAALGELGGRISSDEIRRIMLE 60

Query: 61  IDTDGDGFIDLDEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE 120
           IDTDGDGFIDLDEF AFH++AYP+GGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE
Sbjct: 61  IDTDGDGFIDLDEFTAFHQDAYPEGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGE 120

Query: 121 KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 155
           KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS
Sbjct: 121 KCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 154

BLAST of CmaCh14G008380 vs. NCBI nr
Match: XP_022143269.1 (probable calcium-binding protein CML23 [Momordica charantia])

HSP 1 Score: 266.9 bits (681), Expect = 1.1e-67
Identity = 134/160 (83.75%), Postives = 142/160 (88.75%), Query Frame = 0

Query: 1   MAKKSVSNP------GALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDET 60
           MAKKSVS+P      GALGSMDEV K+F KFDKNGDGKISV+ELG ALGELGGK+SSDE 
Sbjct: 3   MAKKSVSDPSSTNLGGALGSMDEVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEV 62

Query: 61  RRIMLEIDTDGDGFIDLDEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFV 120
           RRIM EID DGDGFIDLDEFA FH++    GGGNKDLQDAFDLYD+DKNGLISAKELH V
Sbjct: 63  RRIMAEIDKDGDGFIDLDEFAEFHQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSV 122

Query: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 155
           LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMM+RS
Sbjct: 123 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 162

BLAST of CmaCh14G008380 vs. NCBI nr
Match: XP_022948508.1 (probable calcium-binding protein CML23 [Cucurbita moschata] >XP_023524703.1 probable calcium-binding protein CML23 [Cucurbita pepo subsp. pepo] >KAG6607012.1 putative calcium-binding protein CML23, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036709.1 putative calcium-binding protein CML23, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 260.0 bits (663), Expect = 1.3e-65
Identity = 131/160 (81.88%), Postives = 140/160 (87.50%), Query Frame = 0

Query: 1   MAKKSVSNP------GALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDET 60
           MAK SVSNP      GALGSMDEV+ +FKKFDKNGDGKIS+ EL AALGELGGKIS DE 
Sbjct: 3   MAKTSVSNPSSPNPGGALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKISDDEI 62

Query: 61  RRIMLEIDTDGDGFIDLDEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFV 120
           RRIM EID DG+GFIDLDEFA FH+ +   GGG+KDLQDAFDLYD+DKNGLISAKELH V
Sbjct: 63  RRIMSEIDKDGNGFIDLDEFAEFHQNSSSTGGGSKDLQDAFDLYDVDKNGLISAKELHSV 122

Query: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMSRS 155
           LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMM+RS
Sbjct: 123 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 162

BLAST of CmaCh14G008380 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 173.3 bits (438), Expect = 1.5e-43
Identity = 88/156 (56.41%), Postives = 112/156 (71.79%), Query Frame = 0

Query: 1   MAKKSVSNPGALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLE 60
           M+ K+      LGSMD++ K+F++FDKNGDGKISV EL   +  L    S +ET  +M +
Sbjct: 1   MSSKNGVVRSCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQ 60

Query: 61  IDTDGDGFIDLDEFAAFHREAYPDGGGNK----DLQDAFDLYDMDKNGLISAKELHFVLK 120
            D DG+GFIDLDEF A  +     GG N+    DL++AF+LYD+D NG ISAKELH V+K
Sbjct: 61  FDLDGNGFIDLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMK 120

Query: 121 RLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMS 153
            LGEKCS++DC +MIS VD+DGDG VNF+EFKKMMS
Sbjct: 121 NLGEKCSVQDCKKMISKVDIDGDGCVNFDEFKKMMS 156

BLAST of CmaCh14G008380 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 167.9 bits (424), Expect = 6.2e-42
Identity = 84/150 (56.00%), Postives = 110/150 (73.33%), Query Frame = 0

Query: 4   KSVSNPGALGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLEIDT 63
           K+VS    LGSM+++ K+F++FDKN DGKIS+ EL   +G L    S +ET+ +M E D 
Sbjct: 3   KNVSR-NCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 62

Query: 64  DGDGFIDLDEFAAFHR--EAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEK 123
           DG+GFIDLDEF A  +  +   +    +DL++AFDLYD+D+NG ISA ELH V+K LGEK
Sbjct: 63  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 122

Query: 124 CSLKDCCRMISSVDVDGDGHVNFEEFKKMM 152
           CS++DC RMI+ VD DGDG V+FEEFKKMM
Sbjct: 123 CSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151

BLAST of CmaCh14G008380 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 157.5 bits (397), Expect = 8.4e-39
Identity = 77/143 (53.85%), Postives = 100/143 (69.93%), Query Frame = 0

Query: 12  LGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLEIDTDGDGFIDL 71
           + + +E+ K+F +FD NGDGKISV ELG     +G   +  E  R++ E+DTD DG+I+L
Sbjct: 18  MANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINL 77

Query: 72  DEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEKCSLKDCCRMI 131
           DEF+   R +        +++DAFDLYD DKNGLISA ELH VL RLG  CS++DC RMI
Sbjct: 78  DEFSTLCRSS----SSAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMI 137

Query: 132 SSVDVDGDGHVNFEEFKKMMSRS 155
             VD DGDG+VNFEEF+KMM+ S
Sbjct: 138 GPVDADGDGNVNFEEFQKMMTSS 156

BLAST of CmaCh14G008380 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 157.5 bits (397), Expect = 8.4e-39
Identity = 77/143 (53.85%), Postives = 100/143 (69.93%), Query Frame = 0

Query: 12  LGSMDEVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLEIDTDGDGFIDL 71
           + + +E+ K+F +FD NGDGKISV ELG     +G   +  E  R++ E+DTD DG+I+L
Sbjct: 18  MANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINL 77

Query: 72  DEFAAFHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEKCSLKDCCRMI 131
           DEF+   R +        +++DAFDLYD DKNGLISA ELH VL RLG  CS++DC RMI
Sbjct: 78  DEFSTLCRSS----SSAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMI 137

Query: 132 SSVDVDGDGHVNFEEFKKMMSRS 155
             VD DGDG+VNFEEF+KMM+ S
Sbjct: 138 GPVDADGDGNVNFEEFQKMMTSS 156

BLAST of CmaCh14G008380 vs. TAIR 10
Match: AT1G73630.1 (EF hand calcium-binding protein family )

HSP 1 Score: 150.2 bits (378), Expect = 1.3e-36
Identity = 75/136 (55.15%), Postives = 96/136 (70.59%), Query Frame = 0

Query: 17  EVMKIFKKFDKNGDGKISVSELGAALGELGGKISSDETRRIMLEIDTDGDGFIDLDEFAA 76
           E+ K+F KFD NGDGKISVSELG     +G   + +E  R++ EID D DGFI+ +EFA 
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEFAT 79

Query: 77  FHREAYPDGGGNKDLQDAFDLYDMDKNGLISAKELHFVLKRLGEKCSLKDCCRMISSVDV 136
             R +        ++++AFDLYD +KNGLIS+ E+H VL RLG  CS++DC RMI  VD 
Sbjct: 80  ICRSS----SSAVEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVDT 139

Query: 137 DGDGHVNFEEFKKMMS 153
           DGDG+VNFEEF+KMMS
Sbjct: 140 DGDGNVNFEEFQKMMS 151

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P250702.1e-4256.41Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2[more]
Q9C8Y18.8e-4156.00Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ... [more]
Q9M7R01.2e-3757.14Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1[more]
Q9LE221.2e-3753.85Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 ... [more]
Q0DJV64.5e-3753.02Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Match NameE-valueIdentityDescription
A0A6J1J8112.4e-81100.00calcium-binding protein CML24-like OS=Cucurbita maxima OX=3661 GN=LOC111482109 P... [more]
A0A6J1F6R41.9e-7895.45calcium-binding protein CML24-like OS=Cucurbita moschata OX=3662 GN=LOC111441384... [more]
A0A6J1CNU45.1e-6883.75probable calcium-binding protein CML23 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1G9H56.3e-6681.88probable calcium-binding protein CML23 OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A6J1KB054.1e-6581.25probable calcium-binding protein CML23 OS=Cucurbita maxima OX=3661 GN=LOC1114932... [more]
Match NameE-valueIdentityDescription
XP_022983538.14.9e-81100.00calcium-binding protein CML24-like [Cucurbita maxima][more]
XP_023526443.11.7e-7896.10calcium-binding protein CML24-like [Cucurbita pepo subsp. pepo][more]
XP_022934113.13.9e-7895.45calcium-binding protein CML24-like [Cucurbita moschata] >KAG6581203.1 putative c... [more]
XP_022143269.11.1e-6783.75probable calcium-binding protein CML23 [Momordica charantia][more]
XP_022948508.11.3e-6581.88probable calcium-binding protein CML23 [Cucurbita moschata] >XP_023524703.1 prob... [more]
Match NameE-valueIdentityDescription
AT5G37770.11.5e-4356.41EF hand calcium-binding protein family [more]
AT1G66400.16.2e-4256.00calmodulin like 23 [more]
AT1G18210.18.4e-3953.85Calcium-binding EF-hand family protein [more]
AT1G18210.28.4e-3953.85Calcium-binding EF-hand family protein [more]
AT1G73630.11.3e-3655.15EF hand calcium-binding protein family [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 126..154
e-value: 2.4E-4
score: 30.5
coord: 90..118
e-value: 1.2E-4
score: 31.5
coord: 53..81
e-value: 8.7E-4
score: 28.6
coord: 17..45
e-value: 2.7E-7
score: 40.2
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 17..77
e-value: 4.9E-14
score: 52.5
coord: 89..152
e-value: 2.7E-10
score: 40.6
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 13..48
score: 16.521873
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 49..84
score: 12.923368
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 86..121
score: 13.676544
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 122..154
score: 13.062845
IPR002048EF-hand domainCDDcd00051EFhcoord: 17..79
e-value: 8.4636E-18
score: 70.6545
IPR002048EF-hand domainCDDcd00051EFhcoord: 90..152
e-value: 4.23889E-18
score: 71.4249
NoneNo IPR availableGENE3D1.10.238.10coord: 89..154
e-value: 1.1E-21
score: 78.9
NoneNo IPR availableGENE3D1.10.238.10coord: 2..88
e-value: 2.5E-21
score: 78.5
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 7..152
NoneNo IPR availablePANTHERPTHR10891:SF794CALCIUM-BINDING PROTEIN CML23-RELATEDcoord: 7..152
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 26..38
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 135..147
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 99..111
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 62..74
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 15..152

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh14G008380.1CmaCh14G008380.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding