Spg017434 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg017434 vs. NCBI nr
Match: XP_022159319.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Momordica charantia])

HSP 1 Score: 753.8 bits (1945), Expect = 9.2e-214
Identity = 365/492 (74.19%), Postives = 424/492 (86.18%), Query Frame = 0

Query: 5   EDFVLAVLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAE 64
           +  VL +L+L+  LF+ ++Y  SPSSKQ  LPPGPKPWPILGNLLQIGE+AH S TRFA+
Sbjct: 11  QQLVLPILLLLASLFITLKYFRSPSSKQTKLPPGPKPWPILGNLLQIGEDAHASMTRFAQ 70

Query: 65  AYGPLISLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPE 124
           AYGPLISLRLG QLLVVASSP AAA VLKTQDRLLSARYIF    D   +RECS +FSPE
Sbjct: 71  AYGPLISLRLGGQLLVVASSPDAAAVVLKTQDRLLSARYIFQTVHDEALHRECSFVFSPE 130

Query: 125 CGEHWKNLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGSVLEVKDFVFTTVFN 184
           C + W+ +RS+CRANLF+ +AI+SQA+LR  KMDEMVEFL+S QGSV+E++DFVFTTVFN
Sbjct: 131 CNDRWRKVRSICRANLFTAKAIDSQANLRRMKMDEMVEFLVSKQGSVVEIRDFVFTTVFN 190

Query: 185 TLSNLIFSRDLFDFVGDGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKSAIY 244
            LSN IFSRDLFDFVGDGF+GIK SLKK+V+LGL PNLAD+Y LLRN D+Q LRKK+ IY
Sbjct: 191 ILSNFIFSRDLFDFVGDGFNGIKASLKKMVTLGLTPNLADYYSLLRNLDLQGLRKKATIY 250

Query: 245 RKEIDSVWEVLIKERRELHRQNA-SLPEQDFLDVMIHSQFNDQQINFLIFELLMAGADTT 304
           RK+IDS W+ LI ERR++H QN+ +   +DFLDV+I SQFND+QIN LI E+ +AG DTT
Sbjct: 251 RKQIDSAWKELIDERRKIHLQNSPNFVARDFLDVLIQSQFNDEQINHLIVEIFLAGTDTT 310

Query: 305 TMTVEWAMAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKETLRLHPPA 364
           TMTVEWAMAE+LK +D M KVREEMK EIN+Q+ +DES +S+LHYLWKCVKETLRLHPPA
Sbjct: 311 TMTVEWAMAEMLKQRDIMAKVREEMKREINNQSTVDESKISQLHYLWKCVKETLRLHPPA 370

Query: 365 PFLLPRVAPMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERFISCDVDFKGY 424
           PFLLPRVAP DCELMGYSIPK TLIFVNVWGIGRDPS+W+DSL+F+PERF  CDVDFKGY
Sbjct: 371 PFLLPRVAPTDCELMGYSIPKDTLIFVNVWGIGRDPSLWEDSLSFNPERFTDCDVDFKGY 430

Query: 425 DYRYLPFGGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLDLNGMLGVTLQK 484
           DYRYLPFGGGRRICPGLPMA VQ+  ILATLIHNFE SLPNG+DFS LDL+G LGVTLQK
Sbjct: 431 DYRYLPFGGGRRICPGLPMASVQIPLILATLIHNFELSLPNGQDFSKLDLDGKLGVTLQK 490

Query: 485 KEPLKLIPKQRI 496
           K+PL+LI ++R+
Sbjct: 491 KKPLQLILQKRL 502

BLAST of Spg017434 vs. NCBI nr
Match: XP_022928791.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata])

HSP 1 Score: 722.2 bits (1863), Expect = 3.0e-204
Identity = 355/484 (73.35%), Postives = 407/484 (84.09%), Query Frame = 0

Query: 11  VLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAEAYGPLI 70
           +L+L+P +F+++R+I+S SSKQPPLPPGPKPWPILGNLLQIG+NAH+S T+F+  YGPLI
Sbjct: 9   LLLLLPLIFIILRHISS-SSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGPLI 68

Query: 71  SLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPECGEHWK 130
           SL+LGAQ LVVASSP+AAAA+LKTQDRLLSARYIF MTPD   +  CSLLFSPECG+ WK
Sbjct: 69  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 128

Query: 131 NLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGSVLEVKDFVFTTVFNTLSNLI 190
           NLRS+CRA LF+ RAIESQASLR +KM+EMVEFL S QGSV+E+K+ VF TVFNTLSN I
Sbjct: 129 NLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 188

Query: 191 FSRDLFDFVGDGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKSAIYRKEIDS 250
           FSRDL  FVGDGF+GIKG  K ++ LGLIPNLADFYPLLR FD+Q LRKK+AIY+ ++DS
Sbjct: 189 FSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQVDS 248

Query: 251 VWEVLIKERRELHRQNASLPEQDFLDVMIHSQFNDQQINFLIFELLMAGADTTTMTVEWA 310
            WE+LIKERR +H Q  S    DFLDV+I S F D+QI++LI E+L AG DTTT TVEWA
Sbjct: 249 SWEILIKERRTIHSQGVS-AASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTTTTTVEWA 308

Query: 311 MAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKETLRLHPPAPFLLPRV 370
           MAELLKN+D M +VREE K  IN    IDES +S+LHYLWKCVKETLRLHPPAPFLLPR 
Sbjct: 309 MAELLKNRDIMNRVREETKKAING-GAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRY 368

Query: 371 APMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERFISCDVDFKGYDYRYLPF 430
           AP  CELMGYS+PK T+IFVNVWGIGRDPSIW+D  +F PERF  CD D KG+DYR+LPF
Sbjct: 369 APEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFLPF 428

Query: 431 GGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLDLNGMLGVTLQKKEPLKLI 490
           GGGRRICPGLPMA VQV  ILATLIHNFEW LPN ED S LDLNG LGVTLQK+EPLKL+
Sbjct: 429 GGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEEPLKLL 488

Query: 491 PKQR 495
            K R
Sbjct: 489 SKNR 489

BLAST of Spg017434 vs. NCBI nr
Match: XP_038892964.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Benincasa hispida])

HSP 1 Score: 716.1 bits (1847), Expect = 2.1e-202
Identity = 348/496 (70.16%), Postives = 418/496 (84.27%), Query Frame = 0

Query: 1   MATHEDFVLAVLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTT 60
           +A+ EDFVL V++L+P LF++ + I+ PSSK+PP+PPGPKPWPILGNLLQIG+NAH+S T
Sbjct: 9   VASQEDFVLCVILLLPLLFVVFKLISFPSSKKPPIPPGPKPWPILGNLLQIGKNAHISIT 68

Query: 61  RFAEAYGPLISLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLL 120
            FA+ YGPLISLRLG +LLVVASSP+AAAAVLKTQDRLLS RY+FHMTPD  Q+ ECSL+
Sbjct: 69  HFAQTYGPLISLRLGGRLLVVASSPAAAAAVLKTQDRLLSNRYVFHMTPDLAQHHECSLV 128

Query: 121 FSPECGEHWKNLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGSVLEVKDFVFT 180
           FSPEC +HW++LRS+C+ANLF+ +AI+SQASLR RKM+EM+EFLLS QGSV+E++DFV T
Sbjct: 129 FSPECNDHWRSLRSICKANLFTAKAIKSQASLRRRKMNEMIEFLLSKQGSVVEIRDFVLT 188

Query: 181 TVFNTLSNLIFSRDLFDFVGDGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKK 240
           TVFN LSN IFSRDL DF G+GF+G+K S   L +LGL PNL DFYP LRN D+Q LR K
Sbjct: 189 TVFNALSNFIFSRDLIDFDGEGFNGLKQSFNNLKNLGLAPNLGDFYPFLRNLDVQGLRNK 248

Query: 241 SAIYRKEIDSVWEVLIKERRELHRQNASLPEQDFLDVMIHSQFNDQQINFLIFELLMAGA 300
            +IY K+I+S  +++I +RR+L   + S P QDFLDVMI SQF +  IN+L+ ELL+AG 
Sbjct: 249 CSIYSKQINSTLDIIINQRRKLASTDPSAP-QDFLDVMIQSQFTNDHINYLVVELLLAGT 308

Query: 301 DTTTMTVEWAMAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKETLRLH 360
           DT+T+TVEW MAELLKNK+ +EKVREE+ TE+  QN IDES + KL YLWKCVKETLRLH
Sbjct: 309 DTSTITVEWTMAELLKNKNIIEKVREEVNTEMK-QNSIDESEIYKLKYLWKCVKETLRLH 368

Query: 361 PPAPFLLPRVAPMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERFISCD-VD 420
           PPAPFLLPR+AP+DCELMGYSIPK +LIFVNVWGIGRDPSIW++SL+F+P RF  CD VD
Sbjct: 369 PPAPFLLPRIAPIDCELMGYSIPKESLIFVNVWGIGRDPSIWEESLSFNPMRFSDCDHVD 428

Query: 421 FKGYDYRYLPFGGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNG-EDFSNLDLNGMLG 480
            KG D+ YLPFGGGRRICPG PMA V +  ILA LIHNFEWSLPNG EDFS +D+ GMLG
Sbjct: 429 LKGNDFGYLPFGGGRRICPGFPMATVLLPLILANLIHNFEWSLPNGDEDFSKMDMEGMLG 488

Query: 481 VTLQKKEPLKLIPKQR 495
           VTL KK+PLKLIPK+R
Sbjct: 489 VTLLKKQPLKLIPKKR 502

BLAST of Spg017434 vs. NCBI nr
Match: KAE8647950.1 (hypothetical protein Csa_000545 [Cucumis sativus])

HSP 1 Score: 714.1 bits (1842), Expect = 8.1e-202
Identity = 350/489 (71.57%), Postives = 408/489 (83.44%), Query Frame = 0

Query: 10  AVLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAEAYGPL 69
           ++L+L+P +F++++ I SPS K+ PLPPGP PWPILGNL QIG+NAH+S T+FA  YGPL
Sbjct: 12  SLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHISITQFANIYGPL 71

Query: 70  ISLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPECGEHW 129
           ISL+LGAQ LVVASSP+ A AVLKT DR LS RYI  MTPD     + SL+FSPECG+HW
Sbjct: 72  ISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHSLVFSPECGDHW 131

Query: 130 KNLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGSVLEVKDFVFTTVFNTLSNL 189
           KNLRS+C+ANLF+ +AIESQA LR +KM E+VEFL S QGSV+++KDFVFT+VFNTLSNL
Sbjct: 132 KNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFVFTSVFNTLSNL 191

Query: 190 IFSRDLFDFVGDGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKSAIYRKEID 249
           IFS DL DFVGDGF+GIKGS KK++ LGL PNLADFYPLLR FD+Q LRKK+AIY  E+D
Sbjct: 192 IFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLRKKAAIYMNEVD 251

Query: 250 SVWEVLIKERRELHRQNASLPEQDFLDVMIHSQFNDQQINFLIFELLMAGADTTTMTVEW 309
           S+W +LIKERRE+H Q  S    DFLDV+I S F+DQQIN+LI ELL A  DTTT TVEW
Sbjct: 252 SLWGILIKERREIHCQQGS-ASSDFLDVLIQSGFSDQQINYLINELLFAATDTTTTTVEW 311

Query: 310 AMAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKETLRLHPPAPFLLPR 369
           AM+E+LK+KD + K REE+K    +   IDESN+SKL YLW CVKETLRLHPP PFLLPR
Sbjct: 312 AMSEILKDKDILNKAREEIKKVAKESPAIDESNISKLQYLWSCVKETLRLHPPVPFLLPR 371

Query: 370 VAPMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERF-ISC--DVDFKGYDYR 429
           +APMDCE+MGYSIPK ++IFVNVWGIGRDPSIW+DS  F+PERF + C  +VDFKGYDYR
Sbjct: 372 LAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGNNVDFKGYDYR 431

Query: 430 YLPFGGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLDLNGMLGVTLQKKEP 489
           YLPFGGGRRICPGLPMAIVQV  ILATL+HNFEWSLPNGED S LDLNG LGVTLQK E 
Sbjct: 432 YLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRLGVTLQKYET 491

Query: 490 LKLIPKQRI 496
           LKLIPK+R+
Sbjct: 492 LKLIPKRRL 499

BLAST of Spg017434 vs. NCBI nr
Match: XP_022970204.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita maxima])

HSP 1 Score: 713.8 bits (1841), Expect = 1.1e-201
Identity = 352/484 (72.73%), Postives = 407/484 (84.09%), Query Frame = 0

Query: 11  VLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAEAYGPLI 70
           +L+L+P +F ++R+I S SSKQPPLPPGP PWPILGNLLQIG+NAH+S T+F+  YGPLI
Sbjct: 9   LLLLLPLIFTILRHICS-SSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQFSHFYGPLI 68

Query: 71  SLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPECGEHWK 130
           SL+LGAQ LVVASSP+AAAA+LKTQDRLLSARYIF MTPD   +  CSLLFSPECG+ WK
Sbjct: 69  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 128

Query: 131 NLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGSVLEVKDFVFTTVFNTLSNLI 190
           NLRS+CRA LF+ RAIESQASLR +KM++MVEFL S QGSV+E+K+ VF TVFNTLSN I
Sbjct: 129 NLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 188

Query: 191 FSRDLFDFVGDGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKSAIYRKEIDS 250
           FSRDL  FVGDGF+GIKGS K ++ LGLIPNLADFYPLLR+FD+Q LRKK+AIY+ ++DS
Sbjct: 189 FSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRKKAAIYKNQVDS 248

Query: 251 VWEVLIKERRELHRQNASLPEQDFLDVMIHSQFNDQQINFLIFELLMAGADTTTMTVEWA 310
            WE+LIKERR +H ++ S    DFLDV+I S F D+QI++LI E+L AG DTTT TVEWA
Sbjct: 249 SWEILIKERRTIHSRDVS-AASDFLDVLIESGFTDEQISYLIVEVLSAGTDTTTTTVEWA 308

Query: 311 MAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKETLRLHPPAPFLLPRV 370
           MAELLKN+D M +VREE K  IN    IDES +S+LHYLWKCVKETLRLHPPAPFLLPR 
Sbjct: 309 MAELLKNRDIMNRVREETKKAING-GAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRY 368

Query: 371 APMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERFISCDVDFKGYDYRYLPF 430
           AP  CELMGYS+PK T+IFVNVWGIGRDPSIW+D  +F PERF   D D KG+DYR+LPF
Sbjct: 369 APEACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFDGFDADLKGHDYRFLPF 428

Query: 431 GGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLDLNGMLGVTLQKKEPLKLI 490
           GGGRRICPGLPMA VQV  ILATLIHNFEW LPN ED S LDLNG LGVTLQK+EPLKL+
Sbjct: 429 GGGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLGVTLQKEEPLKLL 488

Query: 491 PKQR 495
            K+R
Sbjct: 489 SKKR 489

BLAST of Spg017434 vs. ExPASy Swiss-Prot
Match: O64899 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia californica OX=3467 GN=CYP80B1 PE=1 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 1.6e-115
Identity = 213/489 (43.56%), Postives = 312/489 (63.80%), Query Frame = 0

Query: 10  AVLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAEAYGPL 69
           + + L+  +   + Y+    S    LPPGPKPWPI+GNLLQ+GE  H      A+ YG +
Sbjct: 3   STVALIAVIISSILYLLFGGSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDI 62

Query: 70  ISLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPECGEHW 129
            +L++G + +VVAS+ SAA+ +LKT DR+LSARY+F  +   + + E S+++S +C E W
Sbjct: 63  FTLKMGTETVVVASTSSAASEILKTHDRILSARYVF-QSFRVKGHVENSIVWS-DCTETW 122

Query: 130 KNLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGSVLEVKDFVFTTVFNTLSNL 189
           KNLR +CR  LF+ + IESQA +R +K +EMVE+L+  QG  +++ + +F T+ N   NL
Sbjct: 123 KNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNL 182

Query: 190 IFSRDLFDFVGD---GFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKSAIYRK 249
           IFS+++F+ +GD   G    K  L +++ LG   N AD++P+L  FD+   RK+ A   K
Sbjct: 183 IFSQNIFE-LGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLK 242

Query: 250 EIDSVWEVLIKERRELHRQNASLPEQDFLDVMIHSQFNDQQINFLIFELLMAGADTTTMT 309
            I ++W  +++ER+   + +    + DF+DV + S  ND QIN L+ EL  AG +T+  T
Sbjct: 243 GIYAIWGAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSAST 302

Query: 310 VEWAMAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKETLRLHPPAPFL 369
           +EWAM EL KN     K+R E++T + +++ + ES+   L YL   VKETLRLHPP P L
Sbjct: 303 IEWAMTELTKNPKITAKIRSEIQTVVGERS-VKESDFPNLPYLEATVKETLRLHPPTPLL 362

Query: 370 LPRVAPMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERFISCDVDFKGYDYR 429
           LPR A   C ++ Y+IPK   I VN WGIGRDP  W D L F PERF++  VDF+G D+ 
Sbjct: 363 LPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWTDPLTFSPERFLNSSVDFRGNDFS 422

Query: 430 YLPFGGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLDLNGMLGVTLQKKEP 489
            +PFG GRRICPGLP+A   +  ++AT + N +W LPNG    +L +    G+TLQK+ P
Sbjct: 423 LIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPP 482

Query: 490 LKLIPKQRI 496
           L ++PK R+
Sbjct: 483 LFIVPKSRV 487

BLAST of Spg017434 vs. ExPASy Swiss-Prot
Match: Q9FXW4 (Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3442 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 1.6e-115
Identity = 222/485 (45.77%), Postives = 310/485 (63.92%), Query Frame = 0

Query: 12  LVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAEAYGPLIS 71
           + +V F FLL  +     S+   LPPGP+P PI+GNLLQ+G+  H    + A+ YG L S
Sbjct: 6   IAIVSFSFLLFLFFILRDSRPKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKYGELFS 65

Query: 72  LRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPECGEHWKN 131
           L+LG+Q +VVASSP+AAA +LKT D++LS RY+F  +   +++ E S+++S EC ++WK 
Sbjct: 66  LKLGSQTVVVASSPAAAAEILKTHDKILSGRYVF-QSFRVKEHVENSIVWS-ECNDNWKL 125

Query: 132 LRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGSVLEVKDFVFTTVFNTLSNLIF 191
           LR +CR  LF+ + IESQ+ +R  K  EMV+FL   +G V+++ + VF T+ N   NLIF
Sbjct: 126 LRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVNIFGNLIF 185

Query: 192 SRDLFDFVG--DGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKSAIYRKEID 251
           S+D+FD      G   +K  L KL+ +G   N AD++P++   D+   R+  A   ++I 
Sbjct: 186 SKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQQIY 245

Query: 252 SVWEVLIKERRELHRQNASLPEQDFLDVMIHSQFNDQQINFLIFELLMAGADTTTMTVEW 311
            VW V++KERR       S  + DF+DV++++  +DQ+IN L+ EL  AG +T+  T+EW
Sbjct: 246 DVWGVMLKERR---GTKGSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSASTIEW 305

Query: 312 AMAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKETLRLHPPAPFLLPR 371
           A+ EL K    + K+R E+   + D N + ES++  L YL   VKETLRLHPP P LLPR
Sbjct: 306 AITELTKKPLVVSKIRLELVNVVGD-NTVKESDLPHLPYLQAFVKETLRLHPPTPLLLPR 365

Query: 372 VAPMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERFISCDVDFKGYDYRYLP 431
            A   C +M Y+IPK   I VN W IGRDP  W D L F PERF+S DVD+KG D+  +P
Sbjct: 366 RALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIP 425

Query: 432 FGGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLDLNGMLGVTLQKKEPLKL 491
           FGGGRRICPGLP+A      I+ATL+ NFEWSLP G   S L ++   G+TLQK  PL +
Sbjct: 426 FGGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLI 484

Query: 492 IPKQR 495
           + K R
Sbjct: 486 VLKAR 484

BLAST of Spg017434 vs. ExPASy Swiss-Prot
Match: O64900 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3467 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 3.5e-115
Identity = 212/489 (43.35%), Postives = 310/489 (63.39%), Query Frame = 0

Query: 9   LAVLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAEAYGP 68
           +  + L+  +   + Y+   SS    LPPGPKPWPI+GNLLQ+GE  H      A+ YG 
Sbjct: 3   VVTVALIAVIISSILYLLFGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGD 62

Query: 69  LISLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPECGEH 128
           + +L++G + +VVAS+ SAA+ +LKT DR+LSARY+F  +   + + E S+++S +C E 
Sbjct: 63  IFTLKMGTETVVVASTSSAASEILKTHDRILSARYVF-QSFRVKGHVENSIVWS-DCTET 122

Query: 129 WKNLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGSVLEVKDFVFTTVFNTLSN 188
           WKNLR +CR  LF+ + IESQA +R +K +EMVE+L+  QG  +++ + +F T+ N   N
Sbjct: 123 WKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGN 182

Query: 189 LIFSRDLFDF--VGDGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKSAIYRK 248
           LIFS+++F+      G    K  L +++ LG   N AD++P+L  FD+   RK+ A   K
Sbjct: 183 LIFSQNIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLK 242

Query: 249 EIDSVWEVLIKERRELHRQNASLPEQDFLDVMIHSQFNDQQINFLIFELLMAGADTTTMT 308
            I ++W  +++ER+   + +    + DF+DV + S  ND QIN L+ EL  AG +T+  T
Sbjct: 243 GIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSAST 302

Query: 309 VEWAMAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKETLRLHPPAPFL 368
           +EWAM EL KN     K+R E++T + +++ + ES+   L YL   VKETLRLHPP P L
Sbjct: 303 IEWAMTELTKNPKITAKLRSELQTVVGERS-VKESDFPNLPYLEATVKETLRLHPPTPLL 362

Query: 369 LPRVAPMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERFISCDVDFKGYDYR 428
           LPR A   C ++ Y+IPK   I VN WGIGRDP  W D L F PERF++  VDF+G D+ 
Sbjct: 363 LPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLNSSVDFRGNDFS 422

Query: 429 YLPFGGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLDLNGMLGVTLQKKEP 488
            +PFG GRRICPGLP+A   +  ++AT + N +W LPNG    +L +    G+TLQK+ P
Sbjct: 423 LIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPP 482

Query: 489 LKLIPKQRI 496
           L ++PK R+
Sbjct: 483 LFIVPKSRV 488

BLAST of Spg017434 vs. ExPASy Swiss-Prot
Match: Q9SP06 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum OX=3469 GN=CYP80B3 PE=1 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 1.2e-112
Identity = 215/489 (43.97%), Postives = 314/489 (64.21%), Query Frame = 0

Query: 9   LAVLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAEAYGP 68
           L  +V+  FL+L+ R      S    LPPGPKPWPI+GNLLQ+GE  H    + AE YG 
Sbjct: 2   LVAVVITTFLYLIFR-----DSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGD 61

Query: 69  LISLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPECGEH 128
           L SL+LG++ +VVAS+P AA+ +LKT DR+LS RY+F  +   +++ E S+++S EC E 
Sbjct: 62  LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVF-QSFRVKEHVENSIVWS-ECNET 121

Query: 129 WKNLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGSVLEVKDFVFTTVFNTLSN 188
           WK LR +CR  LF+ + IESQA +R  K  EMVE+L  N G+ +++ + VF T+ N   N
Sbjct: 122 WKKLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGN 181

Query: 189 LIFSRDLFDFVGDGFHG---IKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKSAIYR 248
           LIFS+++F  +GD   G   +K  L +++ LG   N AD++P L  FD+    K  A   
Sbjct: 182 LIFSQNIFK-LGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCL 241

Query: 249 KEIDSVWEVLIKERRELHRQNASLPEQDFLDVMIHSQFNDQQINFLIFELLMAGADTTTM 308
           + I SVW  ++KER+ + +Q+ +  + DF+++++ S  +DQQIN L+ E+  AG +T+  
Sbjct: 242 QGIYSVWGAMLKERK-IAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSAS 301

Query: 309 TVEWAMAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKETLRLHPPAPF 368
           T+EWA++EL KN      +R E+ + +  +  + ES++  + YL   VKETLRLHP  P 
Sbjct: 302 TIEWALSELTKNPQVTANMRLELLSVVG-KRPVKESDIPNMPYLQAFVKETLRLHPATPL 361

Query: 369 LLPRVAPMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERFISCDVDFKGYDY 428
           LLPR A   C+++ Y+IPK   I VN WGIGRDP  W D L F PERF++  +DFKG D+
Sbjct: 362 LLPRRALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDF 421

Query: 429 RYLPFGGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLDLNGMLGVTLQKKE 488
             +PFG GRRICPG+P+A   ++ I+++L+ NF+W  P G D S L +    G+TLQK+ 
Sbjct: 422 ELIPFGAGRRICPGVPLATQFISLIVSSLVQNFDWGFPKGMDPSQLIMEEKFGLTLQKEP 480

Query: 489 PLKLIPKQR 495
           PL ++PK R
Sbjct: 482 PLYIVPKTR 480

BLAST of Spg017434 vs. ExPASy Swiss-Prot
Match: A8CDR5 (Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 2.3e-103
Identity = 198/495 (40.00%), Postives = 310/495 (62.63%), Query Frame = 0

Query: 6   DFVLAVLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENA----HVSTTR 65
           D  +A+  L+P + + +  +     K   LPPGP PWP++GNL  +  N     H++   
Sbjct: 2   DLQIALFSLIPVILVFILLL---KPKYKNLPPGPHPWPLIGNLPILFTNTEVPLHITLAN 61

Query: 66  FAEAYGPLISLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLF 125
            A  +GP++ L LG Q  V+AS+  AA  +LKT DR+ SAR+I  M+   + + + SL++
Sbjct: 62  MARTHGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHI-RMSFRLKHHIKYSLVW 121

Query: 126 SPECGEHWKNLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGSVLEVKDFVFTT 185
           S +C ++WK LR + R  +FS + +++Q+ +R +K+ E+++FL S +G V+++  FVF T
Sbjct: 122 S-DCTDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFVFGT 181

Query: 186 VFNTLSNLIFSRDLFDFVGDGFH--GIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRK 245
           + N L N++FS+D+F + GD     GI+  +++++ +G  PN+A+FYP L   D+Q L+K
Sbjct: 182 LLNILGNVVFSKDVFVY-GDETDKGGIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLKK 241

Query: 246 KSAIYRKEIDSVWEVLIKERRELHRQNASLPEQDFLDVMIHSQFNDQQINFLIFELLMAG 305
           K       +  +WE  +KER    + N +   +D LDV++ + FND QIN L  E    G
Sbjct: 242 KCDERFIRVMKMWEGTVKER----KANRNEESKDMLDVLLANDFNDAQINALFLETFGPG 301

Query: 306 ADTTTMTVEWAMAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKETLRL 365
           ++T++ T+EW +AEL+K+   M KVR+E+  E+   + I ES++ +L YL  C+KE +RL
Sbjct: 302 SETSSATIEWVIAELIKSPKEMAKVRKEL-NEVVGTSTIKESDLPQLPYLQACIKEAMRL 361

Query: 366 HPPAPFLLPRVAPMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERFISCDVD 425
           HP APFLLPR A   CE+MGY+IPK + + VN + IGRDP  WKD   F PERF+  DVD
Sbjct: 362 HPAAPFLLPRRAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFLESDVD 421

Query: 426 FKGYDYRYLPFGGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLDLNGMLGV 485
           F G  Y+++PFG GRR C G+P+A   +  I+ +L+HN+++ LP G    +L +N ML +
Sbjct: 422 FHGAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMNEMLSL 481

Query: 486 TLQKKEPLKLIPKQR 495
           TL     L ++PK R
Sbjct: 482 TLAIDPSLCVVPKAR 485

BLAST of Spg017434 vs. ExPASy TrEMBL
Match: A0A6J1E237 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Momordica charantia OX=3673 GN=LOC111025729 PE=3 SV=1)

HSP 1 Score: 753.8 bits (1945), Expect = 4.5e-214
Identity = 365/492 (74.19%), Postives = 424/492 (86.18%), Query Frame = 0

Query: 5   EDFVLAVLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAE 64
           +  VL +L+L+  LF+ ++Y  SPSSKQ  LPPGPKPWPILGNLLQIGE+AH S TRFA+
Sbjct: 11  QQLVLPILLLLASLFITLKYFRSPSSKQTKLPPGPKPWPILGNLLQIGEDAHASMTRFAQ 70

Query: 65  AYGPLISLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPE 124
           AYGPLISLRLG QLLVVASSP AAA VLKTQDRLLSARYIF    D   +RECS +FSPE
Sbjct: 71  AYGPLISLRLGGQLLVVASSPDAAAVVLKTQDRLLSARYIFQTVHDEALHRECSFVFSPE 130

Query: 125 CGEHWKNLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGSVLEVKDFVFTTVFN 184
           C + W+ +RS+CRANLF+ +AI+SQA+LR  KMDEMVEFL+S QGSV+E++DFVFTTVFN
Sbjct: 131 CNDRWRKVRSICRANLFTAKAIDSQANLRRMKMDEMVEFLVSKQGSVVEIRDFVFTTVFN 190

Query: 185 TLSNLIFSRDLFDFVGDGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKSAIY 244
            LSN IFSRDLFDFVGDGF+GIK SLKK+V+LGL PNLAD+Y LLRN D+Q LRKK+ IY
Sbjct: 191 ILSNFIFSRDLFDFVGDGFNGIKASLKKMVTLGLTPNLADYYSLLRNLDLQGLRKKATIY 250

Query: 245 RKEIDSVWEVLIKERRELHRQNA-SLPEQDFLDVMIHSQFNDQQINFLIFELLMAGADTT 304
           RK+IDS W+ LI ERR++H QN+ +   +DFLDV+I SQFND+QIN LI E+ +AG DTT
Sbjct: 251 RKQIDSAWKELIDERRKIHLQNSPNFVARDFLDVLIQSQFNDEQINHLIVEIFLAGTDTT 310

Query: 305 TMTVEWAMAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKETLRLHPPA 364
           TMTVEWAMAE+LK +D M KVREEMK EIN+Q+ +DES +S+LHYLWKCVKETLRLHPPA
Sbjct: 311 TMTVEWAMAEMLKQRDIMAKVREEMKREINNQSTVDESKISQLHYLWKCVKETLRLHPPA 370

Query: 365 PFLLPRVAPMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERFISCDVDFKGY 424
           PFLLPRVAP DCELMGYSIPK TLIFVNVWGIGRDPS+W+DSL+F+PERF  CDVDFKGY
Sbjct: 371 PFLLPRVAPTDCELMGYSIPKDTLIFVNVWGIGRDPSLWEDSLSFNPERFTDCDVDFKGY 430

Query: 425 DYRYLPFGGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLDLNGMLGVTLQK 484
           DYRYLPFGGGRRICPGLPMA VQ+  ILATLIHNFE SLPNG+DFS LDL+G LGVTLQK
Sbjct: 431 DYRYLPFGGGRRICPGLPMASVQIPLILATLIHNFELSLPNGQDFSKLDLDGKLGVTLQK 490

Query: 485 KEPLKLIPKQRI 496
           K+PL+LI ++R+
Sbjct: 491 KKPLQLILQKRL 502

BLAST of Spg017434 vs. ExPASy TrEMBL
Match: A0A6J1ELA6 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435609 PE=3 SV=1)

HSP 1 Score: 722.2 bits (1863), Expect = 1.4e-204
Identity = 355/484 (73.35%), Postives = 407/484 (84.09%), Query Frame = 0

Query: 11  VLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAEAYGPLI 70
           +L+L+P +F+++R+I+S SSKQPPLPPGPKPWPILGNLLQIG+NAH+S T+F+  YGPLI
Sbjct: 9   LLLLLPLIFIILRHISS-SSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGPLI 68

Query: 71  SLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPECGEHWK 130
           SL+LGAQ LVVASSP+AAAA+LKTQDRLLSARYIF MTPD   +  CSLLFSPECG+ WK
Sbjct: 69  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 128

Query: 131 NLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGSVLEVKDFVFTTVFNTLSNLI 190
           NLRS+CRA LF+ RAIESQASLR +KM+EMVEFL S QGSV+E+K+ VF TVFNTLSN I
Sbjct: 129 NLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 188

Query: 191 FSRDLFDFVGDGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKSAIYRKEIDS 250
           FSRDL  FVGDGF+GIKG  K ++ LGLIPNLADFYPLLR FD+Q LRKK+AIY+ ++DS
Sbjct: 189 FSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQVDS 248

Query: 251 VWEVLIKERRELHRQNASLPEQDFLDVMIHSQFNDQQINFLIFELLMAGADTTTMTVEWA 310
            WE+LIKERR +H Q  S    DFLDV+I S F D+QI++LI E+L AG DTTT TVEWA
Sbjct: 249 SWEILIKERRTIHSQGVS-AASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTTTTTVEWA 308

Query: 311 MAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKETLRLHPPAPFLLPRV 370
           MAELLKN+D M +VREE K  IN    IDES +S+LHYLWKCVKETLRLHPPAPFLLPR 
Sbjct: 309 MAELLKNRDIMNRVREETKKAING-GAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRY 368

Query: 371 APMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERFISCDVDFKGYDYRYLPF 430
           AP  CELMGYS+PK T+IFVNVWGIGRDPSIW+D  +F PERF  CD D KG+DYR+LPF
Sbjct: 369 APEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFLPF 428

Query: 431 GGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLDLNGMLGVTLQKKEPLKLI 490
           GGGRRICPGLPMA VQV  ILATLIHNFEW LPN ED S LDLNG LGVTLQK+EPLKL+
Sbjct: 429 GGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEEPLKLL 488

Query: 491 PKQR 495
            K R
Sbjct: 489 SKNR 489

BLAST of Spg017434 vs. ExPASy TrEMBL
Match: A0A6J1I4V7 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=3661 GN=LOC111469167 PE=3 SV=1)

HSP 1 Score: 713.8 bits (1841), Expect = 5.1e-202
Identity = 352/484 (72.73%), Postives = 407/484 (84.09%), Query Frame = 0

Query: 11  VLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAEAYGPLI 70
           +L+L+P +F ++R+I S SSKQPPLPPGP PWPILGNLLQIG+NAH+S T+F+  YGPLI
Sbjct: 9   LLLLLPLIFTILRHICS-SSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQFSHFYGPLI 68

Query: 71  SLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPECGEHWK 130
           SL+LGAQ LVVASSP+AAAA+LKTQDRLLSARYIF MTPD   +  CSLLFSPECG+ WK
Sbjct: 69  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 128

Query: 131 NLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGSVLEVKDFVFTTVFNTLSNLI 190
           NLRS+CRA LF+ RAIESQASLR +KM++MVEFL S QGSV+E+K+ VF TVFNTLSN I
Sbjct: 129 NLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 188

Query: 191 FSRDLFDFVGDGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKSAIYRKEIDS 250
           FSRDL  FVGDGF+GIKGS K ++ LGLIPNLADFYPLLR+FD+Q LRKK+AIY+ ++DS
Sbjct: 189 FSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRKKAAIYKNQVDS 248

Query: 251 VWEVLIKERRELHRQNASLPEQDFLDVMIHSQFNDQQINFLIFELLMAGADTTTMTVEWA 310
            WE+LIKERR +H ++ S    DFLDV+I S F D+QI++LI E+L AG DTTT TVEWA
Sbjct: 249 SWEILIKERRTIHSRDVS-AASDFLDVLIESGFTDEQISYLIVEVLSAGTDTTTTTVEWA 308

Query: 311 MAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKETLRLHPPAPFLLPRV 370
           MAELLKN+D M +VREE K  IN    IDES +S+LHYLWKCVKETLRLHPPAPFLLPR 
Sbjct: 309 MAELLKNRDIMNRVREETKKAING-GAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRY 368

Query: 371 APMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERFISCDVDFKGYDYRYLPF 430
           AP  CELMGYS+PK T+IFVNVWGIGRDPSIW+D  +F PERF   D D KG+DYR+LPF
Sbjct: 369 APEACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFDGFDADLKGHDYRFLPF 428

Query: 431 GGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLDLNGMLGVTLQKKEPLKLI 490
           GGGRRICPGLPMA VQV  ILATLIHNFEW LPN ED S LDLNG LGVTLQK+EPLKL+
Sbjct: 429 GGGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLGVTLQKEEPLKLL 488

Query: 491 PKQR 495
            K+R
Sbjct: 489 SKKR 489

BLAST of Spg017434 vs. ExPASy TrEMBL
Match: A0A5A7UIU3 (Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G001030 PE=3 SV=1)

HSP 1 Score: 713.0 bits (1839), Expect = 8.7e-202
Identity = 352/490 (71.84%), Postives = 413/490 (84.29%), Query Frame = 0

Query: 10  AVLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAEAYGPL 69
           ++L+L+P +F+++++I SPSSK  PLPPGP PWPILGNLLQIG+NAH+S T+FA  YGPL
Sbjct: 12  SLLLLLP-IFIILKHIKSPSSKL-PLPPGPNPWPILGNLLQIGQNAHISITQFANIYGPL 71

Query: 70  ISLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPECGEHW 129
           ISL+LGAQ L+VASSP+AA AVLKTQDRLLSARYIF MTPD   + + SL+FSPECG+ W
Sbjct: 72  ISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFSPECGDQW 131

Query: 130 KNLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGSVLEVKDFVFTTVFNTLSNL 189
           KNLRSLC+ NLF+ +AIESQA LR +KM E+V+FL S QGSV+EVKDFVFT+VFNTLSNL
Sbjct: 132 KNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFVFTSVFNTLSNL 191

Query: 190 IFSRDLFDFVGDGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKSAIYRKEID 249
           IFS DL DFVGDGF GIKG  KK++ LGL PNLADFYPLLR FD+Q L+ K+ IY+ E+D
Sbjct: 192 IFSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKADIYKNEVD 251

Query: 250 SVWEVLIKERRELHRQNASLPEQDFLDVMIHSQFNDQQINFLIFELLMAGADTTTMTVEW 309
           S+W +LIKERRE+H Q  S    DFLDV+I S F+DQQIN LI ELL AG DTTT TVEW
Sbjct: 252 SLWGILIKERREIHCQQGS-ASNDFLDVLIQSGFDDQQINALIIELLSAGTDTTTTTVEW 311

Query: 310 AMAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKETLRLHPPAPFLLPR 369
           AM+E+LK+KD + KVREEMK    +   IDESN+S+L YLW+CVKETLRLHPPAPFLLPR
Sbjct: 312 AMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRLHPPAPFLLPR 371

Query: 370 VAPMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERF-ISC---DVDFKGYDY 429
           +APMDCE+MGYSIPK +++FVNVWGIGRDPS+W+DS  F+P+RF + C   +VDFKGYDY
Sbjct: 372 LAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGNDNVDFKGYDY 431

Query: 430 RYLPFGGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLDLNGMLGVTLQKKE 489
           RYLPFGGGRRICPGLPMAIVQV  ILATLIHNFEWSLPN ED S LDLNG LGVTLQK E
Sbjct: 432 RYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGRLGVTLQKYE 491

Query: 490 PLKLIPKQRI 496
            LKLIPK+R+
Sbjct: 492 TLKLIPKRRV 498

BLAST of Spg017434 vs. ExPASy TrEMBL
Match: A0A1S3CT14 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656 GN=LOC103504626 PE=3 SV=1)

HSP 1 Score: 713.0 bits (1839), Expect = 8.7e-202
Identity = 352/490 (71.84%), Postives = 413/490 (84.29%), Query Frame = 0

Query: 10  AVLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAEAYGPL 69
           ++L+L+P +F+++++I SPSSK  PLPPGP PWPILGNLLQIG+NAH+S T+FA  YGPL
Sbjct: 12  SLLLLLP-IFIILKHIKSPSSKL-PLPPGPNPWPILGNLLQIGQNAHISITQFANIYGPL 71

Query: 70  ISLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPECGEHW 129
           ISL+LGAQ L+VASSP+AA AVLKTQDRLLSARYIF MTPD   + + SL+FSPECG+ W
Sbjct: 72  ISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFSPECGDQW 131

Query: 130 KNLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGSVLEVKDFVFTTVFNTLSNL 189
           KNLRSLC+ NLF+ +AIESQA LR +KM E+V+FL S QGSV+EVKDFVFT+VFNTLSNL
Sbjct: 132 KNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFVFTSVFNTLSNL 191

Query: 190 IFSRDLFDFVGDGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKSAIYRKEID 249
           IFS DL DFVGDGF GIKG  KK++ LGL PNLADFYPLLR FD+Q L+ K+ IY+ E+D
Sbjct: 192 IFSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKADIYKNEVD 251

Query: 250 SVWEVLIKERRELHRQNASLPEQDFLDVMIHSQFNDQQINFLIFELLMAGADTTTMTVEW 309
           S+W +LIKERRE+H Q  S    DFLDV+I S F+DQQIN LI ELL AG DTTT TVEW
Sbjct: 252 SLWGILIKERREIHCQQGS-ASNDFLDVLIQSGFDDQQINALIIELLSAGTDTTTTTVEW 311

Query: 310 AMAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKETLRLHPPAPFLLPR 369
           AM+E+LK+KD + KVREEMK    +   IDESN+S+L YLW+CVKETLRLHPPAPFLLPR
Sbjct: 312 AMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRLHPPAPFLLPR 371

Query: 370 VAPMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERF-ISC---DVDFKGYDY 429
           +APMDCE+MGYSIPK +++FVNVWGIGRDPS+W+DS  F+P+RF + C   +VDFKGYDY
Sbjct: 372 LAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGNDNVDFKGYDY 431

Query: 430 RYLPFGGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLDLNGMLGVTLQKKE 489
           RYLPFGGGRRICPGLPMAIVQV  ILATLIHNFEWSLPN ED S LDLNG LGVTLQK E
Sbjct: 432 RYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGRLGVTLQKYE 491

Query: 490 PLKLIPKQRI 496
            LKLIPK+R+
Sbjct: 492 TLKLIPKRRV 498

BLAST of Spg017434 vs. TAIR 10
Match: AT2G45570.1 (cytochrome P450, family 76, subfamily C, polypeptide 2 )

HSP 1 Score: 339.7 bits (870), Expect = 3.9e-93
Identity = 188/504 (37.30%), Postives = 289/504 (57.34%), Query Frame = 0

Query: 7   FVLAVLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAEAY 66
           F L   VL  F+          S K  P PPGP   PI+GN+  +G N H S    ++ Y
Sbjct: 10  FPLFCFVLSFFIIFFTTTRPRSSRKVVPSPPGPPRLPIIGNIHLVGRNPHHSFADLSKTY 69

Query: 67  GPLISLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQ----QNRECSLLFS 126
           GP++SL+ G+   VV +SP AA  VL+T D++LS+R     TP        + + S+++ 
Sbjct: 70  GPIMSLKFGSLNTVVVTSPEAAREVLRTYDQILSSR-----TPTNSIRSINHDKVSVVWL 129

Query: 127 PECGEHWKNLRSLCRANLFSGRAIESQASLRGRKMDEMVEFL--LSNQGSVLEVKDFVFT 186
           P     W+ LR L    LFS + IE+  +LR  K+ E+V F+   S +   +++    F 
Sbjct: 130 PPSSSRWRLLRKLSATQLFSPQRIEATKTLRENKVKELVSFMSESSEREEAVDISRATFI 189

Query: 187 TVFNTLSNLIFSRDLFDFVGDGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKK 246
           T  N +SN++FS DL ++  +     + ++  ++     P+ A+F+P L   D+Q  RK 
Sbjct: 190 TALNIISNILFSVDLGNYDSNKSGVFQDTVIGVMEAVGNPDAANFFPFLGFLDLQGNRKT 249

Query: 247 SAIYRKEIDSVW----EVLIKERRELHRQNASLPEQDFLDVMI------HSQFNDQQINF 306
                + +  V+    +  + E+      +  + E+DF+DV++       ++ N   I  
Sbjct: 250 LKACSERLFKVFRGFIDAKLAEKSLRDTNSKDVRERDFVDVLLDLTEGDEAELNTNDIVH 309

Query: 307 LIFELLMAGADTTTMTVEWAMAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLW 366
           L+ +L  AG DT + TVEWAMAELL+N +TM K + E+   I  + +++ES++S L YL 
Sbjct: 310 LLLDLFGAGTDTNSSTVEWAMAELLRNPETMVKAQAEIDCVIGQKGVVEESDISALPYLQ 369

Query: 367 KCVKETLRLHPPAPFLLPRVAPMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDP 426
             VKET RLHP AP L+PR A  D E++G+ +PK T +FVNVW IGRDP++W++S  F P
Sbjct: 370 AVVKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVFVNVWAIGRDPNVWENSSRFKP 429

Query: 427 ERFISCDVDFKGYDYRYLPFGGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSN 486
           ERF+  D+D +G DY   PFG GRRICPGLP+A+  V  +LA+L+++F+W LPNG    +
Sbjct: 430 ERFLGKDIDLRGRDYELTPFGAGRRICPGLPLAVKTVPLMLASLLYSFDWKLPNGVGSED 489

Query: 487 LDLNGMLGVTLQKKEPLKLIPKQR 495
           LD++   G+TL K  PL  +P ++
Sbjct: 490 LDMDETFGLTLHKTNPLHAVPVKK 508

BLAST of Spg017434 vs. TAIR 10
Match: AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4 )

HSP 1 Score: 331.6 bits (849), Expect = 1.0e-90
Identity = 181/502 (36.06%), Postives = 289/502 (57.57%), Query Frame = 0

Query: 7   FVLAVLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAEAY 66
           F+L   +   FL          S +   LPPGP   PI+GN+ Q+G+N H S    A+ Y
Sbjct: 10  FLLFCFISSCFLISTTARSRRSSGRAATLPPGPPRLPIIGNIHQVGKNPHSSFADLAKIY 69

Query: 67  GPLISLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQ---NRECSLLFSP 126
           GP++SL+ G    VV +SP AA  VL+T D++LS R     + D+ +   + E S+++ P
Sbjct: 70  GPIMSLKFGCLNSVVITSPEAAREVLRTHDQILSGR----KSNDSIRCFGHEEVSVIWLP 129

Query: 127 ECGEHWKNLRSLCRANLFSGRAIESQASLRGRKMDEMVEFL--LSNQGSVLEVKDFVFTT 186
                W+ LR L    +FS +  E+  +LR +K+ E+V F+   S +   +++    +TT
Sbjct: 130 PSSARWRMLRKLSVTLMFSPQRTEATKALRMKKVQELVSFMNESSERKEAVDISRASYTT 189

Query: 187 VFNTLSNLIFSRDLFDFVGDGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKS 246
           V N +SN++FS DL  +     +  + ++   +     P+ A+++P +   D+Q  RK  
Sbjct: 190 VLNIISNILFSVDLGSYDSKKSNEFQDTVIGAMEAAGKPDAANYFPFMGFLDLQGNRKAM 249

Query: 247 AIYRKEIDSVWEVLIKER---RELHRQNASLPEQDFLDVMI------HSQFNDQQINFLI 306
               + +  V+   +  +   + L   +  +  +DFLD ++       ++ ++  I  L+
Sbjct: 250 RGLTERLFRVFRGFMDAKIAEKSLGNYSKDVSNRDFLDSLLILNEGDEAELDNNDIEHLL 309

Query: 307 FELLMAGADTTTMTVEWAMAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKC 366
            ++  AG DT++ T+EWAMAELL+N  TM K + EM   +   +++ ES++S L YL   
Sbjct: 310 LDMFTAGTDTSSSTLEWAMAELLRNPKTMVKAQAEMDRVLGQNSVVQESDISGLPYLQAV 369

Query: 367 VKETLRLHPPAPFLLPRVAPMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPER 426
           VKET RLHP AP L+PR A  D E++G+ +PK T + VNVW IGRDPS+W++   F+PER
Sbjct: 370 VKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVLVNVWAIGRDPSVWENPSQFEPER 429

Query: 427 FISCDVDFKGYDYRYLPFGGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLD 486
           F+  D+D KG DY   PFGGGRRICPGLP+A+  V+ +LA+L+++F+W LPNG    +LD
Sbjct: 430 FMGKDIDVKGRDYELTPFGGGRRICPGLPLAVKTVSLMLASLLYSFDWKLPNGVVSEDLD 489

Query: 487 LNGMLGVTLQKKEPLKLIPKQR 495
           ++   G+TL +   L  IP ++
Sbjct: 490 MDETFGITLHRTNTLYAIPVKK 507

BLAST of Spg017434 vs. TAIR 10
Match: AT3G52970.1 (cytochrome P450, family 76, subfamily G, polypeptide 1 )

HSP 1 Score: 331.3 bits (848), Expect = 1.4e-90
Identity = 187/503 (37.18%), Postives = 283/503 (56.26%), Query Frame = 0

Query: 9   LAVLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQI-GENAHVSTTRFAEAYG 68
           +AVL+ V  LF   R        +  LPPGP PWP++GN+ Q+ G   H S T+ +  +G
Sbjct: 17  IAVLIYVTCLFYTKRC-------RTRLPPGPNPWPVIGNIFQLAGLPPHDSLTKLSRRHG 76

Query: 69  PLISLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPECGE 128
           P+++LR+G+ L VV SS   A  + K  D  L+ R I+      + +     L + + G 
Sbjct: 77  PIMTLRIGSMLTVVISSSEVAREIFKKHDAALAGRKIYEAMKGGKSSD--GSLITAQYGA 136

Query: 129 HWKNLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLL---SNQGSVLEVKDFVFTTVFN 188
           +W+ LR LC    F  R +++ + +R R +D+M+ F+     N    ++V  + F   FN
Sbjct: 137 YWRMLRRLCTTQFFVTRRLDAMSDVRSRCVDQMLRFVEEGGQNGTKTIDVGRYFFLMAFN 196

Query: 189 TLSNLIFSRDLFDFVGDGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKSAIY 248
            + NL+FSRDL D              K++     PN+ADF+PLLR  D Q +R+K+  +
Sbjct: 197 LIGNLMFSRDLLDPDSKRGSEFFYHTGKVMEFAGKPNVADFFPLLRFLDPQGIRRKTQFH 256

Query: 249 RKEIDSVWEVLIKERREL-HRQNASLPEQDFLDVMIH-----------SQFNDQQINFLI 308
            ++   +    I+ER E+  R+ +    +D+LDV++            S F+ + IN ++
Sbjct: 257 VEKAFEIAGEFIRERTEVREREKSDEKTKDYLDVLLEFRGGDGVDEEPSSFSSRDINVIV 316

Query: 309 FELLMAGADTTTMTVEWAMAELLKNKDTMEKVREEMKTEINDQN-IIDESNVSKLHYLWK 368
           FE+  AG DTTT T+EWA+AELL N  T+ K++ E++T     N  + E ++  L YL  
Sbjct: 317 FEMFTAGTDTTTSTLEWALAELLHNPRTLTKLQTELRTYFKSSNQKLQEEDLPNLPYLSA 376

Query: 369 CVKETLRLHPPAPFLLPRVAPMDCELMG-YSIPKGTLIFVNVWGIGRDPSIWKDSLAFDP 428
            + ETLRLHPP PFL+P  A   C +   Y+IPK T + VNVW IGRDP  W D + F P
Sbjct: 377 VIMETLRLHPPLPFLVPHKAMSTCHIFDQYTIPKETQVLVNVWAIGRDPKTWIDPIMFKP 436

Query: 429 ERFISCD--VDFKGYDYRYLPFGGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDF 488
           ERFIS     DFKG DY +LPFG GRR+CP LP+A   +   + +++ +F+W+L NG + 
Sbjct: 437 ERFISDPDARDFKGQDYEFLPFGSGRRMCPALPLASRVLPLAIGSMVRSFDWALENGLNA 496

Query: 489 SNLDLNGMLGVTLQKKEPLKLIP 492
             +D+   +G+TL+K  PL+ IP
Sbjct: 497 EEMDMGERIGITLKKAVPLEAIP 510

BLAST of Spg017434 vs. TAIR 10
Match: AT2G45560.1 (cytochrome P450, family 76, subfamily C, polypeptide 1 )

HSP 1 Score: 325.5 bits (833), Expect = 7.5e-89
Identity = 181/498 (36.35%), Postives = 285/498 (57.23%), Query Frame = 0

Query: 8   VLAVLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAEAYG 67
           +L   +L  FL          S     LPPGP   PI+GN+  +G++ H S    ++ YG
Sbjct: 11  LLFCFILSCFLIFTTTRSGRISRGATALPPGPPRLPIIGNIHLVGKHPHRSFAELSKTYG 70

Query: 68  PLISLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPECGE 127
           P++SL+LG+   VV +SP AA  VL+T D++LSAR   +       +++ SL++ P    
Sbjct: 71  PVMSLKLGSLNTVVIASPEAAREVLRTHDQILSARSPTNAVRSI-NHQDASLVWLPSSSA 130

Query: 128 HWKNLRSLCRANLFSGRAIESQASLRGRKMDEMVEFL--LSNQGSVLEVKDFVFTTVFNT 187
            W+ LR L    L S + IE+  +LR  K+ E+V F+   S++   +++    F T  N 
Sbjct: 131 RWRLLRRLSVTQLLSPQRIEATKALRMNKVKELVSFISESSDREESVDISRVAFITTLNI 190

Query: 188 LSNLIFSRDLFDF-VGDGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKSAIY 247
           +SN++FS DL  +      +G++ ++  ++     P+ A+++P LR  D+Q   K   + 
Sbjct: 191 ISNILFSVDLGSYNAKASINGVQDTVISVMDAAGTPDAANYFPFLRFLDLQGNVKTFKVC 250

Query: 248 RKEIDSVWEVLIKER-RELHRQN--ASLPEQDFLDVMI-----HSQFNDQQINFLIFELL 307
            + +  V+   I  +  E   QN    + + DF+D ++      S+ +   I  L+ ++ 
Sbjct: 251 TERLVRVFRGFIDAKIAEKSSQNNPKDVSKNDFVDNLLDYKGDESELSISDIEHLLLDMF 310

Query: 308 MAGADTTTMTVEWAMAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKET 367
            AG DT++ T+EWAM ELLKN  TM K + E+   I    I++ES++SKL YL   VKET
Sbjct: 311 TAGTDTSSSTLEWAMTELLKNPKTMAKAQAEIDCVIGQNGIVEESDISKLPYLQAVVKET 370

Query: 368 LRLHPPAPFLLPRVAPMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERFISC 427
            RLH P P L+PR A  D E++G+ + K T + VNVW IGRDPS+W +   F+PERF+  
Sbjct: 371 FRLHTPVPLLIPRKAESDAEILGFMVLKDTQVLVNVWAIGRDPSVWDNPSQFEPERFLGK 430

Query: 428 DVDFKGYDYRYLPFGGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLDLNGM 487
           D+D +G DY   PFG GRRICPG+P+A+  V+ +LA+L+++F+W LP G    +LD++  
Sbjct: 431 DMDVRGRDYELTPFGAGRRICPGMPLAMKTVSLMLASLLYSFDWKLPKGVLSEDLDMDET 490

Query: 488 LGVTLQKKEPLKLIPKQR 495
            G+TL K  PL  +P ++
Sbjct: 491 FGLTLHKTNPLHAVPVKK 507

BLAST of Spg017434 vs. TAIR 10
Match: AT3G52970.2 (cytochrome P450, family 76, subfamily G, polypeptide 1 )

HSP 1 Score: 323.9 bits (829), Expect = 2.2e-88
Identity = 186/517 (35.98%), Postives = 283/517 (54.74%), Query Frame = 0

Query: 9   LAVLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQI-GENAHVSTTRFAEAYG 68
           +AVL+ V  LF   R        +  LPPGP PWP++GN+ Q+ G   H S T+ +  +G
Sbjct: 17  IAVLIYVTCLFYTKRC-------RTRLPPGPNPWPVIGNIFQLAGLPPHDSLTKLSRRHG 76

Query: 69  PLISLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPECGE 128
           P+++LR+G+ L VV SS   A  + K  D  L+ R I+      + +     L + + G 
Sbjct: 77  PIMTLRIGSMLTVVISSSEVAREIFKKHDAALAGRKIYEAMKGGKSSD--GSLITAQYGA 136

Query: 129 HWKNLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGS----------------- 188
           +W+ LR LC    F  R +++ + +R R +D+M+ F+     +                 
Sbjct: 137 YWRMLRRLCTTQFFVTRRLDAMSDVRSRCVDQMLRFVEEGGQNGMYYTINNIMTCIICTK 196

Query: 189 VLEVKDFVFTTVFNTLSNLIFSRDLFDFVGDGFHGIKGSLKKLVSLGLIPNLADFYPLLR 248
            ++V  + F   FN + NL+FSRDL D              K++     PN+ADF+PLLR
Sbjct: 197 TIDVGRYFFLMAFNLIGNLMFSRDLLDPDSKRGSEFFYHTGKVMEFAGKPNVADFFPLLR 256

Query: 249 NFDIQRLRKKSAIYRKEIDSVWEVLIKERREL-HRQNASLPEQDFLDVMIH--------- 308
             D Q +R+K+  + ++   +    I+ER E+  R+ +    +D+LDV++          
Sbjct: 257 FLDPQGIRRKTQFHVEKAFEIAGEFIRERTEVREREKSDEKTKDYLDVLLEFRGGDGVDE 316

Query: 309 --SQFNDQQINFLIFELLMAGADTTTMTVEWAMAELLKNKDTMEKVREEMKTEINDQN-I 368
             S F+ + IN ++FE+  AG DTTT T+EWA+AELL N  T+ K++ E++T     N  
Sbjct: 317 EPSSFSSRDINVIVFEMFTAGTDTTTSTLEWALAELLHNPRTLTKLQTELRTYFKSSNQK 376

Query: 369 IDESNVSKLHYLWKCVKETLRLHPPAPFLLPRVAPMDCELMG-YSIPKGTLIFVNVWGIG 428
           + E ++  L YL   + ETLRLHPP PFL+P  A   C +   Y+IPK T + VNVW IG
Sbjct: 377 LQEEDLPNLPYLSAVIMETLRLHPPLPFLVPHKAMSTCHIFDQYTIPKETQVLVNVWAIG 436

Query: 429 RDPSIWKDSLAFDPERFISCD--VDFKGYDYRYLPFGGGRRICPGLPMAIVQVTSILATL 488
           RDP  W D + F PERFIS     DFKG DY +LPFG GRR+CP LP+A   +   + ++
Sbjct: 437 RDPKTWIDPIMFKPERFISDPDARDFKGQDYEFLPFGSGRRMCPALPLASRVLPLAIGSM 496

Query: 489 IHNFEWSLPNGEDFSNLDLNGMLGVTLQKKEPLKLIP 492
           + +F+W+L NG +   +D+   +G+TL+K  PL+ IP
Sbjct: 497 VRSFDWALENGLNAEEMDMGERIGITLKKAVPLEAIP 524