Homology
BLAST of Spg017434.1 vs. NCBI nr
Match:
XP_022159319.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Momordica charantia])
HSP 1 Score: 753.8 bits (1945), Expect = 9.2e-214
Identity = 365/492 (74.19%), Postives = 424/492 (86.18%), Query Frame = 0
Query: 5 EDFVLAVLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAE 64
+ VL +L+L+ LF+ ++Y SPSSKQ LPPGPKPWPILGNLLQIGE+AH S TRFA+
Sbjct: 11 QQLVLPILLLLASLFITLKYFRSPSSKQTKLPPGPKPWPILGNLLQIGEDAHASMTRFAQ 70
Query: 65 AYGPLISLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPE 124
AYGPLISLRLG QLLVVASSP AAA VLKTQDRLLSARYIF D +RECS +FSPE
Sbjct: 71 AYGPLISLRLGGQLLVVASSPDAAAVVLKTQDRLLSARYIFQTVHDEALHRECSFVFSPE 130
Query: 125 CGEHWKNLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGSVLEVKDFVFTTVFN 184
C + W+ +RS+CRANLF+ +AI+SQA+LR KMDEMVEFL+S QGSV+E++DFVFTTVFN
Sbjct: 131 CNDRWRKVRSICRANLFTAKAIDSQANLRRMKMDEMVEFLVSKQGSVVEIRDFVFTTVFN 190
Query: 185 TLSNLIFSRDLFDFVGDGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKSAIY 244
LSN IFSRDLFDFVGDGF+GIK SLKK+V+LGL PNLAD+Y LLRN D+Q LRKK+ IY
Sbjct: 191 ILSNFIFSRDLFDFVGDGFNGIKASLKKMVTLGLTPNLADYYSLLRNLDLQGLRKKATIY 250
Query: 245 RKEIDSVWEVLIKERRELHRQNA-SLPEQDFLDVMIHSQFNDQQINFLIFELLMAGADTT 304
RK+IDS W+ LI ERR++H QN+ + +DFLDV+I SQFND+QIN LI E+ +AG DTT
Sbjct: 251 RKQIDSAWKELIDERRKIHLQNSPNFVARDFLDVLIQSQFNDEQINHLIVEIFLAGTDTT 310
Query: 305 TMTVEWAMAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKETLRLHPPA 364
TMTVEWAMAE+LK +D M KVREEMK EIN+Q+ +DES +S+LHYLWKCVKETLRLHPPA
Sbjct: 311 TMTVEWAMAEMLKQRDIMAKVREEMKREINNQSTVDESKISQLHYLWKCVKETLRLHPPA 370
Query: 365 PFLLPRVAPMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERFISCDVDFKGY 424
PFLLPRVAP DCELMGYSIPK TLIFVNVWGIGRDPS+W+DSL+F+PERF CDVDFKGY
Sbjct: 371 PFLLPRVAPTDCELMGYSIPKDTLIFVNVWGIGRDPSLWEDSLSFNPERFTDCDVDFKGY 430
Query: 425 DYRYLPFGGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLDLNGMLGVTLQK 484
DYRYLPFGGGRRICPGLPMA VQ+ ILATLIHNFE SLPNG+DFS LDL+G LGVTLQK
Sbjct: 431 DYRYLPFGGGRRICPGLPMASVQIPLILATLIHNFELSLPNGQDFSKLDLDGKLGVTLQK 490
Query: 485 KEPLKLIPKQRI 496
K+PL+LI ++R+
Sbjct: 491 KKPLQLILQKRL 502
BLAST of Spg017434.1 vs. NCBI nr
Match:
XP_022928791.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata])
HSP 1 Score: 722.2 bits (1863), Expect = 3.0e-204
Identity = 355/484 (73.35%), Postives = 407/484 (84.09%), Query Frame = 0
Query: 11 VLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAEAYGPLI 70
+L+L+P +F+++R+I+S SSKQPPLPPGPKPWPILGNLLQIG+NAH+S T+F+ YGPLI
Sbjct: 9 LLLLLPLIFIILRHISS-SSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGPLI 68
Query: 71 SLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPECGEHWK 130
SL+LGAQ LVVASSP+AAAA+LKTQDRLLSARYIF MTPD + CSLLFSPECG+ WK
Sbjct: 69 SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 128
Query: 131 NLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGSVLEVKDFVFTTVFNTLSNLI 190
NLRS+CRA LF+ RAIESQASLR +KM+EMVEFL S QGSV+E+K+ VF TVFNTLSN I
Sbjct: 129 NLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 188
Query: 191 FSRDLFDFVGDGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKSAIYRKEIDS 250
FSRDL FVGDGF+GIKG K ++ LGLIPNLADFYPLLR FD+Q LRKK+AIY+ ++DS
Sbjct: 189 FSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQVDS 248
Query: 251 VWEVLIKERRELHRQNASLPEQDFLDVMIHSQFNDQQINFLIFELLMAGADTTTMTVEWA 310
WE+LIKERR +H Q S DFLDV+I S F D+QI++LI E+L AG DTTT TVEWA
Sbjct: 249 SWEILIKERRTIHSQGVS-AASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTTTTTVEWA 308
Query: 311 MAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKETLRLHPPAPFLLPRV 370
MAELLKN+D M +VREE K IN IDES +S+LHYLWKCVKETLRLHPPAPFLLPR
Sbjct: 309 MAELLKNRDIMNRVREETKKAING-GAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRY 368
Query: 371 APMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERFISCDVDFKGYDYRYLPF 430
AP CELMGYS+PK T+IFVNVWGIGRDPSIW+D +F PERF CD D KG+DYR+LPF
Sbjct: 369 APEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFLPF 428
Query: 431 GGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLDLNGMLGVTLQKKEPLKLI 490
GGGRRICPGLPMA VQV ILATLIHNFEW LPN ED S LDLNG LGVTLQK+EPLKL+
Sbjct: 429 GGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEEPLKLL 488
Query: 491 PKQR 495
K R
Sbjct: 489 SKNR 489
BLAST of Spg017434.1 vs. NCBI nr
Match:
XP_038892964.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Benincasa hispida])
HSP 1 Score: 716.1 bits (1847), Expect = 2.1e-202
Identity = 348/496 (70.16%), Postives = 418/496 (84.27%), Query Frame = 0
Query: 1 MATHEDFVLAVLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTT 60
+A+ EDFVL V++L+P LF++ + I+ PSSK+PP+PPGPKPWPILGNLLQIG+NAH+S T
Sbjct: 9 VASQEDFVLCVILLLPLLFVVFKLISFPSSKKPPIPPGPKPWPILGNLLQIGKNAHISIT 68
Query: 61 RFAEAYGPLISLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLL 120
FA+ YGPLISLRLG +LLVVASSP+AAAAVLKTQDRLLS RY+FHMTPD Q+ ECSL+
Sbjct: 69 HFAQTYGPLISLRLGGRLLVVASSPAAAAAVLKTQDRLLSNRYVFHMTPDLAQHHECSLV 128
Query: 121 FSPECGEHWKNLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGSVLEVKDFVFT 180
FSPEC +HW++LRS+C+ANLF+ +AI+SQASLR RKM+EM+EFLLS QGSV+E++DFV T
Sbjct: 129 FSPECNDHWRSLRSICKANLFTAKAIKSQASLRRRKMNEMIEFLLSKQGSVVEIRDFVLT 188
Query: 181 TVFNTLSNLIFSRDLFDFVGDGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKK 240
TVFN LSN IFSRDL DF G+GF+G+K S L +LGL PNL DFYP LRN D+Q LR K
Sbjct: 189 TVFNALSNFIFSRDLIDFDGEGFNGLKQSFNNLKNLGLAPNLGDFYPFLRNLDVQGLRNK 248
Query: 241 SAIYRKEIDSVWEVLIKERRELHRQNASLPEQDFLDVMIHSQFNDQQINFLIFELLMAGA 300
+IY K+I+S +++I +RR+L + S P QDFLDVMI SQF + IN+L+ ELL+AG
Sbjct: 249 CSIYSKQINSTLDIIINQRRKLASTDPSAP-QDFLDVMIQSQFTNDHINYLVVELLLAGT 308
Query: 301 DTTTMTVEWAMAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKETLRLH 360
DT+T+TVEW MAELLKNK+ +EKVREE+ TE+ QN IDES + KL YLWKCVKETLRLH
Sbjct: 309 DTSTITVEWTMAELLKNKNIIEKVREEVNTEMK-QNSIDESEIYKLKYLWKCVKETLRLH 368
Query: 361 PPAPFLLPRVAPMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERFISCD-VD 420
PPAPFLLPR+AP+DCELMGYSIPK +LIFVNVWGIGRDPSIW++SL+F+P RF CD VD
Sbjct: 369 PPAPFLLPRIAPIDCELMGYSIPKESLIFVNVWGIGRDPSIWEESLSFNPMRFSDCDHVD 428
Query: 421 FKGYDYRYLPFGGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNG-EDFSNLDLNGMLG 480
KG D+ YLPFGGGRRICPG PMA V + ILA LIHNFEWSLPNG EDFS +D+ GMLG
Sbjct: 429 LKGNDFGYLPFGGGRRICPGFPMATVLLPLILANLIHNFEWSLPNGDEDFSKMDMEGMLG 488
Query: 481 VTLQKKEPLKLIPKQR 495
VTL KK+PLKLIPK+R
Sbjct: 489 VTLLKKQPLKLIPKKR 502
BLAST of Spg017434.1 vs. NCBI nr
Match:
KAE8647950.1 (hypothetical protein Csa_000545 [Cucumis sativus])
HSP 1 Score: 714.1 bits (1842), Expect = 8.1e-202
Identity = 350/489 (71.57%), Postives = 408/489 (83.44%), Query Frame = 0
Query: 10 AVLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAEAYGPL 69
++L+L+P +F++++ I SPS K+ PLPPGP PWPILGNL QIG+NAH+S T+FA YGPL
Sbjct: 12 SLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHISITQFANIYGPL 71
Query: 70 ISLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPECGEHW 129
ISL+LGAQ LVVASSP+ A AVLKT DR LS RYI MTPD + SL+FSPECG+HW
Sbjct: 72 ISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHSLVFSPECGDHW 131
Query: 130 KNLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGSVLEVKDFVFTTVFNTLSNL 189
KNLRS+C+ANLF+ +AIESQA LR +KM E+VEFL S QGSV+++KDFVFT+VFNTLSNL
Sbjct: 132 KNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFVFTSVFNTLSNL 191
Query: 190 IFSRDLFDFVGDGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKSAIYRKEID 249
IFS DL DFVGDGF+GIKGS KK++ LGL PNLADFYPLLR FD+Q LRKK+AIY E+D
Sbjct: 192 IFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLRKKAAIYMNEVD 251
Query: 250 SVWEVLIKERRELHRQNASLPEQDFLDVMIHSQFNDQQINFLIFELLMAGADTTTMTVEW 309
S+W +LIKERRE+H Q S DFLDV+I S F+DQQIN+LI ELL A DTTT TVEW
Sbjct: 252 SLWGILIKERREIHCQQGS-ASSDFLDVLIQSGFSDQQINYLINELLFAATDTTTTTVEW 311
Query: 310 AMAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKETLRLHPPAPFLLPR 369
AM+E+LK+KD + K REE+K + IDESN+SKL YLW CVKETLRLHPP PFLLPR
Sbjct: 312 AMSEILKDKDILNKAREEIKKVAKESPAIDESNISKLQYLWSCVKETLRLHPPVPFLLPR 371
Query: 370 VAPMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERF-ISC--DVDFKGYDYR 429
+APMDCE+MGYSIPK ++IFVNVWGIGRDPSIW+DS F+PERF + C +VDFKGYDYR
Sbjct: 372 LAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGNNVDFKGYDYR 431
Query: 430 YLPFGGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLDLNGMLGVTLQKKEP 489
YLPFGGGRRICPGLPMAIVQV ILATL+HNFEWSLPNGED S LDLNG LGVTLQK E
Sbjct: 432 YLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRLGVTLQKYET 491
Query: 490 LKLIPKQRI 496
LKLIPK+R+
Sbjct: 492 LKLIPKRRL 499
BLAST of Spg017434.1 vs. NCBI nr
Match:
XP_022970204.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita maxima])
HSP 1 Score: 713.8 bits (1841), Expect = 1.1e-201
Identity = 352/484 (72.73%), Postives = 407/484 (84.09%), Query Frame = 0
Query: 11 VLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAEAYGPLI 70
+L+L+P +F ++R+I S SSKQPPLPPGP PWPILGNLLQIG+NAH+S T+F+ YGPLI
Sbjct: 9 LLLLLPLIFTILRHICS-SSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQFSHFYGPLI 68
Query: 71 SLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPECGEHWK 130
SL+LGAQ LVVASSP+AAAA+LKTQDRLLSARYIF MTPD + CSLLFSPECG+ WK
Sbjct: 69 SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 128
Query: 131 NLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGSVLEVKDFVFTTVFNTLSNLI 190
NLRS+CRA LF+ RAIESQASLR +KM++MVEFL S QGSV+E+K+ VF TVFNTLSN I
Sbjct: 129 NLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 188
Query: 191 FSRDLFDFVGDGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKSAIYRKEIDS 250
FSRDL FVGDGF+GIKGS K ++ LGLIPNLADFYPLLR+FD+Q LRKK+AIY+ ++DS
Sbjct: 189 FSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRKKAAIYKNQVDS 248
Query: 251 VWEVLIKERRELHRQNASLPEQDFLDVMIHSQFNDQQINFLIFELLMAGADTTTMTVEWA 310
WE+LIKERR +H ++ S DFLDV+I S F D+QI++LI E+L AG DTTT TVEWA
Sbjct: 249 SWEILIKERRTIHSRDVS-AASDFLDVLIESGFTDEQISYLIVEVLSAGTDTTTTTVEWA 308
Query: 311 MAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKETLRLHPPAPFLLPRV 370
MAELLKN+D M +VREE K IN IDES +S+LHYLWKCVKETLRLHPPAPFLLPR
Sbjct: 309 MAELLKNRDIMNRVREETKKAING-GAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRY 368
Query: 371 APMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERFISCDVDFKGYDYRYLPF 430
AP CELMGYS+PK T+IFVNVWGIGRDPSIW+D +F PERF D D KG+DYR+LPF
Sbjct: 369 APEACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFDGFDADLKGHDYRFLPF 428
Query: 431 GGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLDLNGMLGVTLQKKEPLKLI 490
GGGRRICPGLPMA VQV ILATLIHNFEW LPN ED S LDLNG LGVTLQK+EPLKL+
Sbjct: 429 GGGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLGVTLQKEEPLKLL 488
Query: 491 PKQR 495
K+R
Sbjct: 489 SKKR 489
BLAST of Spg017434.1 vs. ExPASy Swiss-Prot
Match:
O64899 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia californica OX=3467 GN=CYP80B1 PE=1 SV=1)
HSP 1 Score: 417.9 bits (1073), Expect = 1.6e-115
Identity = 213/489 (43.56%), Postives = 312/489 (63.80%), Query Frame = 0
Query: 10 AVLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAEAYGPL 69
+ + L+ + + Y+ S LPPGPKPWPI+GNLLQ+GE H A+ YG +
Sbjct: 3 STVALIAVIISSILYLLFGGSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDI 62
Query: 70 ISLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPECGEHW 129
+L++G + +VVAS+ SAA+ +LKT DR+LSARY+F + + + E S+++S +C E W
Sbjct: 63 FTLKMGTETVVVASTSSAASEILKTHDRILSARYVF-QSFRVKGHVENSIVWS-DCTETW 122
Query: 130 KNLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGSVLEVKDFVFTTVFNTLSNL 189
KNLR +CR LF+ + IESQA +R +K +EMVE+L+ QG +++ + +F T+ N NL
Sbjct: 123 KNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNL 182
Query: 190 IFSRDLFDFVGD---GFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKSAIYRK 249
IFS+++F+ +GD G K L +++ LG N AD++P+L FD+ RK+ A K
Sbjct: 183 IFSQNIFE-LGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLK 242
Query: 250 EIDSVWEVLIKERRELHRQNASLPEQDFLDVMIHSQFNDQQINFLIFELLMAGADTTTMT 309
I ++W +++ER+ + + + DF+DV + S ND QIN L+ EL AG +T+ T
Sbjct: 243 GIYAIWGAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSAST 302
Query: 310 VEWAMAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKETLRLHPPAPFL 369
+EWAM EL KN K+R E++T + +++ + ES+ L YL VKETLRLHPP P L
Sbjct: 303 IEWAMTELTKNPKITAKIRSEIQTVVGERS-VKESDFPNLPYLEATVKETLRLHPPTPLL 362
Query: 370 LPRVAPMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERFISCDVDFKGYDYR 429
LPR A C ++ Y+IPK I VN WGIGRDP W D L F PERF++ VDF+G D+
Sbjct: 363 LPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWTDPLTFSPERFLNSSVDFRGNDFS 422
Query: 430 YLPFGGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLDLNGMLGVTLQKKEP 489
+PFG GRRICPGLP+A + ++AT + N +W LPNG +L + G+TLQK+ P
Sbjct: 423 LIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPP 482
Query: 490 LKLIPKQRI 496
L ++PK R+
Sbjct: 483 LFIVPKSRV 487
BLAST of Spg017434.1 vs. ExPASy Swiss-Prot
Match:
Q9FXW4 (Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3442 GN=CYP80B2 PE=2 SV=1)
HSP 1 Score: 417.9 bits (1073), Expect = 1.6e-115
Identity = 222/485 (45.77%), Postives = 310/485 (63.92%), Query Frame = 0
Query: 12 LVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAEAYGPLIS 71
+ +V F FLL + S+ LPPGP+P PI+GNLLQ+G+ H + A+ YG L S
Sbjct: 6 IAIVSFSFLLFLFFILRDSRPKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKYGELFS 65
Query: 72 LRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPECGEHWKN 131
L+LG+Q +VVASSP+AAA +LKT D++LS RY+F + +++ E S+++S EC ++WK
Sbjct: 66 LKLGSQTVVVASSPAAAAEILKTHDKILSGRYVF-QSFRVKEHVENSIVWS-ECNDNWKL 125
Query: 132 LRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGSVLEVKDFVFTTVFNTLSNLIF 191
LR +CR LF+ + IESQ+ +R K EMV+FL +G V+++ + VF T+ N NLIF
Sbjct: 126 LRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVNIFGNLIF 185
Query: 192 SRDLFDFVG--DGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKSAIYRKEID 251
S+D+FD G +K L KL+ +G N AD++P++ D+ R+ A ++I
Sbjct: 186 SKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQQIY 245
Query: 252 SVWEVLIKERRELHRQNASLPEQDFLDVMIHSQFNDQQINFLIFELLMAGADTTTMTVEW 311
VW V++KERR S + DF+DV++++ +DQ+IN L+ EL AG +T+ T+EW
Sbjct: 246 DVWGVMLKERR---GTKGSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSASTIEW 305
Query: 312 AMAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKETLRLHPPAPFLLPR 371
A+ EL K + K+R E+ + D N + ES++ L YL VKETLRLHPP P LLPR
Sbjct: 306 AITELTKKPLVVSKIRLELVNVVGD-NTVKESDLPHLPYLQAFVKETLRLHPPTPLLLPR 365
Query: 372 VAPMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERFISCDVDFKGYDYRYLP 431
A C +M Y+IPK I VN W IGRDP W D L F PERF+S DVD+KG D+ +P
Sbjct: 366 RALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIP 425
Query: 432 FGGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLDLNGMLGVTLQKKEPLKL 491
FGGGRRICPGLP+A I+ATL+ NFEWSLP G S L ++ G+TLQK PL +
Sbjct: 426 FGGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLI 484
Query: 492 IPKQR 495
+ K R
Sbjct: 486 VLKAR 484
BLAST of Spg017434.1 vs. ExPASy Swiss-Prot
Match:
O64900 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3467 GN=CYP80B2 PE=2 SV=1)
HSP 1 Score: 416.8 bits (1070), Expect = 3.5e-115
Identity = 212/489 (43.35%), Postives = 310/489 (63.39%), Query Frame = 0
Query: 9 LAVLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAEAYGP 68
+ + L+ + + Y+ SS LPPGPKPWPI+GNLLQ+GE H A+ YG
Sbjct: 3 VVTVALIAVIISSILYLLFGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGD 62
Query: 69 LISLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPECGEH 128
+ +L++G + +VVAS+ SAA+ +LKT DR+LSARY+F + + + E S+++S +C E
Sbjct: 63 IFTLKMGTETVVVASTSSAASEILKTHDRILSARYVF-QSFRVKGHVENSIVWS-DCTET 122
Query: 129 WKNLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGSVLEVKDFVFTTVFNTLSN 188
WKNLR +CR LF+ + IESQA +R +K +EMVE+L+ QG +++ + +F T+ N N
Sbjct: 123 WKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGN 182
Query: 189 LIFSRDLFDF--VGDGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKSAIYRK 248
LIFS+++F+ G K L +++ LG N AD++P+L FD+ RK+ A K
Sbjct: 183 LIFSQNIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLK 242
Query: 249 EIDSVWEVLIKERRELHRQNASLPEQDFLDVMIHSQFNDQQINFLIFELLMAGADTTTMT 308
I ++W +++ER+ + + + DF+DV + S ND QIN L+ EL AG +T+ T
Sbjct: 243 GIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSAST 302
Query: 309 VEWAMAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKETLRLHPPAPFL 368
+EWAM EL KN K+R E++T + +++ + ES+ L YL VKETLRLHPP P L
Sbjct: 303 IEWAMTELTKNPKITAKLRSELQTVVGERS-VKESDFPNLPYLEATVKETLRLHPPTPLL 362
Query: 369 LPRVAPMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERFISCDVDFKGYDYR 428
LPR A C ++ Y+IPK I VN WGIGRDP W D L F PERF++ VDF+G D+
Sbjct: 363 LPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLNSSVDFRGNDFS 422
Query: 429 YLPFGGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLDLNGMLGVTLQKKEP 488
+PFG GRRICPGLP+A + ++AT + N +W LPNG +L + G+TLQK+ P
Sbjct: 423 LIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPP 482
Query: 489 LKLIPKQRI 496
L ++PK R+
Sbjct: 483 LFIVPKSRV 488
BLAST of Spg017434.1 vs. ExPASy Swiss-Prot
Match:
Q9SP06 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum OX=3469 GN=CYP80B3 PE=1 SV=1)
HSP 1 Score: 408.3 bits (1048), Expect = 1.2e-112
Identity = 215/489 (43.97%), Postives = 314/489 (64.21%), Query Frame = 0
Query: 9 LAVLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAEAYGP 68
L +V+ FL+L+ R S LPPGPKPWPI+GNLLQ+GE H + AE YG
Sbjct: 2 LVAVVITTFLYLIFR-----DSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGD 61
Query: 69 LISLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPECGEH 128
L SL+LG++ +VVAS+P AA+ +LKT DR+LS RY+F + +++ E S+++S EC E
Sbjct: 62 LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVF-QSFRVKEHVENSIVWS-ECNET 121
Query: 129 WKNLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGSVLEVKDFVFTTVFNTLSN 188
WK LR +CR LF+ + IESQA +R K EMVE+L N G+ +++ + VF T+ N N
Sbjct: 122 WKKLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGN 181
Query: 189 LIFSRDLFDFVGDGFHG---IKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKSAIYR 248
LIFS+++F +GD G +K L +++ LG N AD++P L FD+ K A
Sbjct: 182 LIFSQNIFK-LGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCL 241
Query: 249 KEIDSVWEVLIKERRELHRQNASLPEQDFLDVMIHSQFNDQQINFLIFELLMAGADTTTM 308
+ I SVW ++KER+ + +Q+ + + DF+++++ S +DQQIN L+ E+ AG +T+
Sbjct: 242 QGIYSVWGAMLKERK-IAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSAS 301
Query: 309 TVEWAMAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKETLRLHPPAPF 368
T+EWA++EL KN +R E+ + + + + ES++ + YL VKETLRLHP P
Sbjct: 302 TIEWALSELTKNPQVTANMRLELLSVVG-KRPVKESDIPNMPYLQAFVKETLRLHPATPL 361
Query: 369 LLPRVAPMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERFISCDVDFKGYDY 428
LLPR A C+++ Y+IPK I VN WGIGRDP W D L F PERF++ +DFKG D+
Sbjct: 362 LLPRRALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDF 421
Query: 429 RYLPFGGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLDLNGMLGVTLQKKE 488
+PFG GRRICPG+P+A ++ I+++L+ NF+W P G D S L + G+TLQK+
Sbjct: 422 ELIPFGAGRRICPGVPLATQFISLIVSSLVQNFDWGFPKGMDPSQLIMEEKFGLTLQKEP 480
Query: 489 PLKLIPKQR 495
PL ++PK R
Sbjct: 482 PLYIVPKTR 480
BLAST of Spg017434.1 vs. ExPASy Swiss-Prot
Match:
A8CDR5 (Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1)
HSP 1 Score: 377.5 bits (968), Expect = 2.3e-103
Identity = 198/495 (40.00%), Postives = 310/495 (62.63%), Query Frame = 0
Query: 6 DFVLAVLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENA----HVSTTR 65
D +A+ L+P + + + + K LPPGP PWP++GNL + N H++
Sbjct: 2 DLQIALFSLIPVILVFILLL---KPKYKNLPPGPHPWPLIGNLPILFTNTEVPLHITLAN 61
Query: 66 FAEAYGPLISLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLF 125
A +GP++ L LG Q V+AS+ AA +LKT DR+ SAR+I M+ + + + SL++
Sbjct: 62 MARTHGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHI-RMSFRLKHHIKYSLVW 121
Query: 126 SPECGEHWKNLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGSVLEVKDFVFTT 185
S +C ++WK LR + R +FS + +++Q+ +R +K+ E+++FL S +G V+++ FVF T
Sbjct: 122 S-DCTDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFVFGT 181
Query: 186 VFNTLSNLIFSRDLFDFVGDGFH--GIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRK 245
+ N L N++FS+D+F + GD GI+ +++++ +G PN+A+FYP L D+Q L+K
Sbjct: 182 LLNILGNVVFSKDVFVY-GDETDKGGIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLKK 241
Query: 246 KSAIYRKEIDSVWEVLIKERRELHRQNASLPEQDFLDVMIHSQFNDQQINFLIFELLMAG 305
K + +WE +KER + N + +D LDV++ + FND QIN L E G
Sbjct: 242 KCDERFIRVMKMWEGTVKER----KANRNEESKDMLDVLLANDFNDAQINALFLETFGPG 301
Query: 306 ADTTTMTVEWAMAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKETLRL 365
++T++ T+EW +AEL+K+ M KVR+E+ E+ + I ES++ +L YL C+KE +RL
Sbjct: 302 SETSSATIEWVIAELIKSPKEMAKVRKEL-NEVVGTSTIKESDLPQLPYLQACIKEAMRL 361
Query: 366 HPPAPFLLPRVAPMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERFISCDVD 425
HP APFLLPR A CE+MGY+IPK + + VN + IGRDP WKD F PERF+ DVD
Sbjct: 362 HPAAPFLLPRRAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFLESDVD 421
Query: 426 FKGYDYRYLPFGGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLDLNGMLGV 485
F G Y+++PFG GRR C G+P+A + I+ +L+HN+++ LP G +L +N ML +
Sbjct: 422 FHGAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMNEMLSL 481
Query: 486 TLQKKEPLKLIPKQR 495
TL L ++PK R
Sbjct: 482 TLAIDPSLCVVPKAR 485
BLAST of Spg017434.1 vs. ExPASy TrEMBL
Match:
A0A6J1E237 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Momordica charantia OX=3673 GN=LOC111025729 PE=3 SV=1)
HSP 1 Score: 753.8 bits (1945), Expect = 4.5e-214
Identity = 365/492 (74.19%), Postives = 424/492 (86.18%), Query Frame = 0
Query: 5 EDFVLAVLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAE 64
+ VL +L+L+ LF+ ++Y SPSSKQ LPPGPKPWPILGNLLQIGE+AH S TRFA+
Sbjct: 11 QQLVLPILLLLASLFITLKYFRSPSSKQTKLPPGPKPWPILGNLLQIGEDAHASMTRFAQ 70
Query: 65 AYGPLISLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPE 124
AYGPLISLRLG QLLVVASSP AAA VLKTQDRLLSARYIF D +RECS +FSPE
Sbjct: 71 AYGPLISLRLGGQLLVVASSPDAAAVVLKTQDRLLSARYIFQTVHDEALHRECSFVFSPE 130
Query: 125 CGEHWKNLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGSVLEVKDFVFTTVFN 184
C + W+ +RS+CRANLF+ +AI+SQA+LR KMDEMVEFL+S QGSV+E++DFVFTTVFN
Sbjct: 131 CNDRWRKVRSICRANLFTAKAIDSQANLRRMKMDEMVEFLVSKQGSVVEIRDFVFTTVFN 190
Query: 185 TLSNLIFSRDLFDFVGDGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKSAIY 244
LSN IFSRDLFDFVGDGF+GIK SLKK+V+LGL PNLAD+Y LLRN D+Q LRKK+ IY
Sbjct: 191 ILSNFIFSRDLFDFVGDGFNGIKASLKKMVTLGLTPNLADYYSLLRNLDLQGLRKKATIY 250
Query: 245 RKEIDSVWEVLIKERRELHRQNA-SLPEQDFLDVMIHSQFNDQQINFLIFELLMAGADTT 304
RK+IDS W+ LI ERR++H QN+ + +DFLDV+I SQFND+QIN LI E+ +AG DTT
Sbjct: 251 RKQIDSAWKELIDERRKIHLQNSPNFVARDFLDVLIQSQFNDEQINHLIVEIFLAGTDTT 310
Query: 305 TMTVEWAMAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKETLRLHPPA 364
TMTVEWAMAE+LK +D M KVREEMK EIN+Q+ +DES +S+LHYLWKCVKETLRLHPPA
Sbjct: 311 TMTVEWAMAEMLKQRDIMAKVREEMKREINNQSTVDESKISQLHYLWKCVKETLRLHPPA 370
Query: 365 PFLLPRVAPMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERFISCDVDFKGY 424
PFLLPRVAP DCELMGYSIPK TLIFVNVWGIGRDPS+W+DSL+F+PERF CDVDFKGY
Sbjct: 371 PFLLPRVAPTDCELMGYSIPKDTLIFVNVWGIGRDPSLWEDSLSFNPERFTDCDVDFKGY 430
Query: 425 DYRYLPFGGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLDLNGMLGVTLQK 484
DYRYLPFGGGRRICPGLPMA VQ+ ILATLIHNFE SLPNG+DFS LDL+G LGVTLQK
Sbjct: 431 DYRYLPFGGGRRICPGLPMASVQIPLILATLIHNFELSLPNGQDFSKLDLDGKLGVTLQK 490
Query: 485 KEPLKLIPKQRI 496
K+PL+LI ++R+
Sbjct: 491 KKPLQLILQKRL 502
BLAST of Spg017434.1 vs. ExPASy TrEMBL
Match:
A0A6J1ELA6 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435609 PE=3 SV=1)
HSP 1 Score: 722.2 bits (1863), Expect = 1.4e-204
Identity = 355/484 (73.35%), Postives = 407/484 (84.09%), Query Frame = 0
Query: 11 VLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAEAYGPLI 70
+L+L+P +F+++R+I+S SSKQPPLPPGPKPWPILGNLLQIG+NAH+S T+F+ YGPLI
Sbjct: 9 LLLLLPLIFIILRHISS-SSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGPLI 68
Query: 71 SLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPECGEHWK 130
SL+LGAQ LVVASSP+AAAA+LKTQDRLLSARYIF MTPD + CSLLFSPECG+ WK
Sbjct: 69 SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 128
Query: 131 NLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGSVLEVKDFVFTTVFNTLSNLI 190
NLRS+CRA LF+ RAIESQASLR +KM+EMVEFL S QGSV+E+K+ VF TVFNTLSN I
Sbjct: 129 NLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 188
Query: 191 FSRDLFDFVGDGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKSAIYRKEIDS 250
FSRDL FVGDGF+GIKG K ++ LGLIPNLADFYPLLR FD+Q LRKK+AIY+ ++DS
Sbjct: 189 FSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQVDS 248
Query: 251 VWEVLIKERRELHRQNASLPEQDFLDVMIHSQFNDQQINFLIFELLMAGADTTTMTVEWA 310
WE+LIKERR +H Q S DFLDV+I S F D+QI++LI E+L AG DTTT TVEWA
Sbjct: 249 SWEILIKERRTIHSQGVS-AASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTTTTTVEWA 308
Query: 311 MAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKETLRLHPPAPFLLPRV 370
MAELLKN+D M +VREE K IN IDES +S+LHYLWKCVKETLRLHPPAPFLLPR
Sbjct: 309 MAELLKNRDIMNRVREETKKAING-GAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRY 368
Query: 371 APMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERFISCDVDFKGYDYRYLPF 430
AP CELMGYS+PK T+IFVNVWGIGRDPSIW+D +F PERF CD D KG+DYR+LPF
Sbjct: 369 APEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDYRFLPF 428
Query: 431 GGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLDLNGMLGVTLQKKEPLKLI 490
GGGRRICPGLPMA VQV ILATLIHNFEW LPN ED S LDLNG LGVTLQK+EPLKL+
Sbjct: 429 GGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEEPLKLL 488
Query: 491 PKQR 495
K R
Sbjct: 489 SKNR 489
BLAST of Spg017434.1 vs. ExPASy TrEMBL
Match:
A0A6J1I4V7 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=3661 GN=LOC111469167 PE=3 SV=1)
HSP 1 Score: 713.8 bits (1841), Expect = 5.1e-202
Identity = 352/484 (72.73%), Postives = 407/484 (84.09%), Query Frame = 0
Query: 11 VLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAEAYGPLI 70
+L+L+P +F ++R+I S SSKQPPLPPGP PWPILGNLLQIG+NAH+S T+F+ YGPLI
Sbjct: 9 LLLLLPLIFTILRHICS-SSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQFSHFYGPLI 68
Query: 71 SLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPECGEHWK 130
SL+LGAQ LVVASSP+AAAA+LKTQDRLLSARYIF MTPD + CSLLFSPECG+ WK
Sbjct: 69 SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 128
Query: 131 NLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGSVLEVKDFVFTTVFNTLSNLI 190
NLRS+CRA LF+ RAIESQASLR +KM++MVEFL S QGSV+E+K+ VF TVFNTLSN I
Sbjct: 129 NLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 188
Query: 191 FSRDLFDFVGDGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKSAIYRKEIDS 250
FSRDL FVGDGF+GIKGS K ++ LGLIPNLADFYPLLR+FD+Q LRKK+AIY+ ++DS
Sbjct: 189 FSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRKKAAIYKNQVDS 248
Query: 251 VWEVLIKERRELHRQNASLPEQDFLDVMIHSQFNDQQINFLIFELLMAGADTTTMTVEWA 310
WE+LIKERR +H ++ S DFLDV+I S F D+QI++LI E+L AG DTTT TVEWA
Sbjct: 249 SWEILIKERRTIHSRDVS-AASDFLDVLIESGFTDEQISYLIVEVLSAGTDTTTTTVEWA 308
Query: 311 MAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKETLRLHPPAPFLLPRV 370
MAELLKN+D M +VREE K IN IDES +S+LHYLWKCVKETLRLHPPAPFLLPR
Sbjct: 309 MAELLKNRDIMNRVREETKKAING-GAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRY 368
Query: 371 APMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERFISCDVDFKGYDYRYLPF 430
AP CELMGYS+PK T+IFVNVWGIGRDPSIW+D +F PERF D D KG+DYR+LPF
Sbjct: 369 APEACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFDGFDADLKGHDYRFLPF 428
Query: 431 GGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLDLNGMLGVTLQKKEPLKLI 490
GGGRRICPGLPMA VQV ILATLIHNFEW LPN ED S LDLNG LGVTLQK+EPLKL+
Sbjct: 429 GGGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLGVTLQKEEPLKLL 488
Query: 491 PKQR 495
K+R
Sbjct: 489 SKKR 489
BLAST of Spg017434.1 vs. ExPASy TrEMBL
Match:
A0A5A7UIU3 (Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G001030 PE=3 SV=1)
HSP 1 Score: 713.0 bits (1839), Expect = 8.7e-202
Identity = 352/490 (71.84%), Postives = 413/490 (84.29%), Query Frame = 0
Query: 10 AVLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAEAYGPL 69
++L+L+P +F+++++I SPSSK PLPPGP PWPILGNLLQIG+NAH+S T+FA YGPL
Sbjct: 12 SLLLLLP-IFIILKHIKSPSSKL-PLPPGPNPWPILGNLLQIGQNAHISITQFANIYGPL 71
Query: 70 ISLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPECGEHW 129
ISL+LGAQ L+VASSP+AA AVLKTQDRLLSARYIF MTPD + + SL+FSPECG+ W
Sbjct: 72 ISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFSPECGDQW 131
Query: 130 KNLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGSVLEVKDFVFTTVFNTLSNL 189
KNLRSLC+ NLF+ +AIESQA LR +KM E+V+FL S QGSV+EVKDFVFT+VFNTLSNL
Sbjct: 132 KNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFVFTSVFNTLSNL 191
Query: 190 IFSRDLFDFVGDGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKSAIYRKEID 249
IFS DL DFVGDGF GIKG KK++ LGL PNLADFYPLLR FD+Q L+ K+ IY+ E+D
Sbjct: 192 IFSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKADIYKNEVD 251
Query: 250 SVWEVLIKERRELHRQNASLPEQDFLDVMIHSQFNDQQINFLIFELLMAGADTTTMTVEW 309
S+W +LIKERRE+H Q S DFLDV+I S F+DQQIN LI ELL AG DTTT TVEW
Sbjct: 252 SLWGILIKERREIHCQQGS-ASNDFLDVLIQSGFDDQQINALIIELLSAGTDTTTTTVEW 311
Query: 310 AMAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKETLRLHPPAPFLLPR 369
AM+E+LK+KD + KVREEMK + IDESN+S+L YLW+CVKETLRLHPPAPFLLPR
Sbjct: 312 AMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRLHPPAPFLLPR 371
Query: 370 VAPMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERF-ISC---DVDFKGYDY 429
+APMDCE+MGYSIPK +++FVNVWGIGRDPS+W+DS F+P+RF + C +VDFKGYDY
Sbjct: 372 LAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGNDNVDFKGYDY 431
Query: 430 RYLPFGGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLDLNGMLGVTLQKKE 489
RYLPFGGGRRICPGLPMAIVQV ILATLIHNFEWSLPN ED S LDLNG LGVTLQK E
Sbjct: 432 RYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGRLGVTLQKYE 491
Query: 490 PLKLIPKQRI 496
LKLIPK+R+
Sbjct: 492 TLKLIPKRRV 498
BLAST of Spg017434.1 vs. ExPASy TrEMBL
Match:
A0A1S3CT14 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656 GN=LOC103504626 PE=3 SV=1)
HSP 1 Score: 713.0 bits (1839), Expect = 8.7e-202
Identity = 352/490 (71.84%), Postives = 413/490 (84.29%), Query Frame = 0
Query: 10 AVLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAEAYGPL 69
++L+L+P +F+++++I SPSSK PLPPGP PWPILGNLLQIG+NAH+S T+FA YGPL
Sbjct: 12 SLLLLLP-IFIILKHIKSPSSKL-PLPPGPNPWPILGNLLQIGQNAHISITQFANIYGPL 71
Query: 70 ISLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPECGEHW 129
ISL+LGAQ L+VASSP+AA AVLKTQDRLLSARYIF MTPD + + SL+FSPECG+ W
Sbjct: 72 ISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFSPECGDQW 131
Query: 130 KNLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGSVLEVKDFVFTTVFNTLSNL 189
KNLRSLC+ NLF+ +AIESQA LR +KM E+V+FL S QGSV+EVKDFVFT+VFNTLSNL
Sbjct: 132 KNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFVFTSVFNTLSNL 191
Query: 190 IFSRDLFDFVGDGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKSAIYRKEID 249
IFS DL DFVGDGF GIKG KK++ LGL PNLADFYPLLR FD+Q L+ K+ IY+ E+D
Sbjct: 192 IFSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKADIYKNEVD 251
Query: 250 SVWEVLIKERRELHRQNASLPEQDFLDVMIHSQFNDQQINFLIFELLMAGADTTTMTVEW 309
S+W +LIKERRE+H Q S DFLDV+I S F+DQQIN LI ELL AG DTTT TVEW
Sbjct: 252 SLWGILIKERREIHCQQGS-ASNDFLDVLIQSGFDDQQINALIIELLSAGTDTTTTTVEW 311
Query: 310 AMAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKETLRLHPPAPFLLPR 369
AM+E+LK+KD + KVREEMK + IDESN+S+L YLW+CVKETLRLHPPAPFLLPR
Sbjct: 312 AMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRLHPPAPFLLPR 371
Query: 370 VAPMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERF-ISC---DVDFKGYDY 429
+APMDCE+MGYSIPK +++FVNVWGIGRDPS+W+DS F+P+RF + C +VDFKGYDY
Sbjct: 372 LAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGNDNVDFKGYDY 431
Query: 430 RYLPFGGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLDLNGMLGVTLQKKE 489
RYLPFGGGRRICPGLPMAIVQV ILATLIHNFEWSLPN ED S LDLNG LGVTLQK E
Sbjct: 432 RYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGRLGVTLQKYE 491
Query: 490 PLKLIPKQRI 496
LKLIPK+R+
Sbjct: 492 TLKLIPKRRV 498
BLAST of Spg017434.1 vs. TAIR 10
Match:
AT2G45570.1 (cytochrome P450, family 76, subfamily C, polypeptide 2 )
HSP 1 Score: 339.7 bits (870), Expect = 3.9e-93
Identity = 188/504 (37.30%), Postives = 289/504 (57.34%), Query Frame = 0
Query: 7 FVLAVLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAEAY 66
F L VL F+ S K P PPGP PI+GN+ +G N H S ++ Y
Sbjct: 10 FPLFCFVLSFFIIFFTTTRPRSSRKVVPSPPGPPRLPIIGNIHLVGRNPHHSFADLSKTY 69
Query: 67 GPLISLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQ----QNRECSLLFS 126
GP++SL+ G+ VV +SP AA VL+T D++LS+R TP + + S+++
Sbjct: 70 GPIMSLKFGSLNTVVVTSPEAAREVLRTYDQILSSR-----TPTNSIRSINHDKVSVVWL 129
Query: 127 PECGEHWKNLRSLCRANLFSGRAIESQASLRGRKMDEMVEFL--LSNQGSVLEVKDFVFT 186
P W+ LR L LFS + IE+ +LR K+ E+V F+ S + +++ F
Sbjct: 130 PPSSSRWRLLRKLSATQLFSPQRIEATKTLRENKVKELVSFMSESSEREEAVDISRATFI 189
Query: 187 TVFNTLSNLIFSRDLFDFVGDGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKK 246
T N +SN++FS DL ++ + + ++ ++ P+ A+F+P L D+Q RK
Sbjct: 190 TALNIISNILFSVDLGNYDSNKSGVFQDTVIGVMEAVGNPDAANFFPFLGFLDLQGNRKT 249
Query: 247 SAIYRKEIDSVW----EVLIKERRELHRQNASLPEQDFLDVMI------HSQFNDQQINF 306
+ + V+ + + E+ + + E+DF+DV++ ++ N I
Sbjct: 250 LKACSERLFKVFRGFIDAKLAEKSLRDTNSKDVRERDFVDVLLDLTEGDEAELNTNDIVH 309
Query: 307 LIFELLMAGADTTTMTVEWAMAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLW 366
L+ +L AG DT + TVEWAMAELL+N +TM K + E+ I + +++ES++S L YL
Sbjct: 310 LLLDLFGAGTDTNSSTVEWAMAELLRNPETMVKAQAEIDCVIGQKGVVEESDISALPYLQ 369
Query: 367 KCVKETLRLHPPAPFLLPRVAPMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDP 426
VKET RLHP AP L+PR A D E++G+ +PK T +FVNVW IGRDP++W++S F P
Sbjct: 370 AVVKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVFVNVWAIGRDPNVWENSSRFKP 429
Query: 427 ERFISCDVDFKGYDYRYLPFGGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSN 486
ERF+ D+D +G DY PFG GRRICPGLP+A+ V +LA+L+++F+W LPNG +
Sbjct: 430 ERFLGKDIDLRGRDYELTPFGAGRRICPGLPLAVKTVPLMLASLLYSFDWKLPNGVGSED 489
Query: 487 LDLNGMLGVTLQKKEPLKLIPKQR 495
LD++ G+TL K PL +P ++
Sbjct: 490 LDMDETFGLTLHKTNPLHAVPVKK 508
BLAST of Spg017434.1 vs. TAIR 10
Match:
AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4 )
HSP 1 Score: 331.6 bits (849), Expect = 1.0e-90
Identity = 181/502 (36.06%), Postives = 289/502 (57.57%), Query Frame = 0
Query: 7 FVLAVLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAEAY 66
F+L + FL S + LPPGP PI+GN+ Q+G+N H S A+ Y
Sbjct: 10 FLLFCFISSCFLISTTARSRRSSGRAATLPPGPPRLPIIGNIHQVGKNPHSSFADLAKIY 69
Query: 67 GPLISLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQ---NRECSLLFSP 126
GP++SL+ G VV +SP AA VL+T D++LS R + D+ + + E S+++ P
Sbjct: 70 GPIMSLKFGCLNSVVITSPEAAREVLRTHDQILSGR----KSNDSIRCFGHEEVSVIWLP 129
Query: 127 ECGEHWKNLRSLCRANLFSGRAIESQASLRGRKMDEMVEFL--LSNQGSVLEVKDFVFTT 186
W+ LR L +FS + E+ +LR +K+ E+V F+ S + +++ +TT
Sbjct: 130 PSSARWRMLRKLSVTLMFSPQRTEATKALRMKKVQELVSFMNESSERKEAVDISRASYTT 189
Query: 187 VFNTLSNLIFSRDLFDFVGDGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKS 246
V N +SN++FS DL + + + ++ + P+ A+++P + D+Q RK
Sbjct: 190 VLNIISNILFSVDLGSYDSKKSNEFQDTVIGAMEAAGKPDAANYFPFMGFLDLQGNRKAM 249
Query: 247 AIYRKEIDSVWEVLIKER---RELHRQNASLPEQDFLDVMI------HSQFNDQQINFLI 306
+ + V+ + + + L + + +DFLD ++ ++ ++ I L+
Sbjct: 250 RGLTERLFRVFRGFMDAKIAEKSLGNYSKDVSNRDFLDSLLILNEGDEAELDNNDIEHLL 309
Query: 307 FELLMAGADTTTMTVEWAMAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKC 366
++ AG DT++ T+EWAMAELL+N TM K + EM + +++ ES++S L YL
Sbjct: 310 LDMFTAGTDTSSSTLEWAMAELLRNPKTMVKAQAEMDRVLGQNSVVQESDISGLPYLQAV 369
Query: 367 VKETLRLHPPAPFLLPRVAPMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPER 426
VKET RLHP AP L+PR A D E++G+ +PK T + VNVW IGRDPS+W++ F+PER
Sbjct: 370 VKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVLVNVWAIGRDPSVWENPSQFEPER 429
Query: 427 FISCDVDFKGYDYRYLPFGGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLD 486
F+ D+D KG DY PFGGGRRICPGLP+A+ V+ +LA+L+++F+W LPNG +LD
Sbjct: 430 FMGKDIDVKGRDYELTPFGGGRRICPGLPLAVKTVSLMLASLLYSFDWKLPNGVVSEDLD 489
Query: 487 LNGMLGVTLQKKEPLKLIPKQR 495
++ G+TL + L IP ++
Sbjct: 490 MDETFGITLHRTNTLYAIPVKK 507
BLAST of Spg017434.1 vs. TAIR 10
Match:
AT3G52970.1 (cytochrome P450, family 76, subfamily G, polypeptide 1 )
HSP 1 Score: 331.3 bits (848), Expect = 1.4e-90
Identity = 187/503 (37.18%), Postives = 283/503 (56.26%), Query Frame = 0
Query: 9 LAVLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQI-GENAHVSTTRFAEAYG 68
+AVL+ V LF R + LPPGP PWP++GN+ Q+ G H S T+ + +G
Sbjct: 17 IAVLIYVTCLFYTKRC-------RTRLPPGPNPWPVIGNIFQLAGLPPHDSLTKLSRRHG 76
Query: 69 PLISLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPECGE 128
P+++LR+G+ L VV SS A + K D L+ R I+ + + L + + G
Sbjct: 77 PIMTLRIGSMLTVVISSSEVAREIFKKHDAALAGRKIYEAMKGGKSSD--GSLITAQYGA 136
Query: 129 HWKNLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLL---SNQGSVLEVKDFVFTTVFN 188
+W+ LR LC F R +++ + +R R +D+M+ F+ N ++V + F FN
Sbjct: 137 YWRMLRRLCTTQFFVTRRLDAMSDVRSRCVDQMLRFVEEGGQNGTKTIDVGRYFFLMAFN 196
Query: 189 TLSNLIFSRDLFDFVGDGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKSAIY 248
+ NL+FSRDL D K++ PN+ADF+PLLR D Q +R+K+ +
Sbjct: 197 LIGNLMFSRDLLDPDSKRGSEFFYHTGKVMEFAGKPNVADFFPLLRFLDPQGIRRKTQFH 256
Query: 249 RKEIDSVWEVLIKERREL-HRQNASLPEQDFLDVMIH-----------SQFNDQQINFLI 308
++ + I+ER E+ R+ + +D+LDV++ S F+ + IN ++
Sbjct: 257 VEKAFEIAGEFIRERTEVREREKSDEKTKDYLDVLLEFRGGDGVDEEPSSFSSRDINVIV 316
Query: 309 FELLMAGADTTTMTVEWAMAELLKNKDTMEKVREEMKTEINDQN-IIDESNVSKLHYLWK 368
FE+ AG DTTT T+EWA+AELL N T+ K++ E++T N + E ++ L YL
Sbjct: 317 FEMFTAGTDTTTSTLEWALAELLHNPRTLTKLQTELRTYFKSSNQKLQEEDLPNLPYLSA 376
Query: 369 CVKETLRLHPPAPFLLPRVAPMDCELMG-YSIPKGTLIFVNVWGIGRDPSIWKDSLAFDP 428
+ ETLRLHPP PFL+P A C + Y+IPK T + VNVW IGRDP W D + F P
Sbjct: 377 VIMETLRLHPPLPFLVPHKAMSTCHIFDQYTIPKETQVLVNVWAIGRDPKTWIDPIMFKP 436
Query: 429 ERFISCD--VDFKGYDYRYLPFGGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDF 488
ERFIS DFKG DY +LPFG GRR+CP LP+A + + +++ +F+W+L NG +
Sbjct: 437 ERFISDPDARDFKGQDYEFLPFGSGRRMCPALPLASRVLPLAIGSMVRSFDWALENGLNA 496
Query: 489 SNLDLNGMLGVTLQKKEPLKLIP 492
+D+ +G+TL+K PL+ IP
Sbjct: 497 EEMDMGERIGITLKKAVPLEAIP 510
BLAST of Spg017434.1 vs. TAIR 10
Match:
AT2G45560.1 (cytochrome P450, family 76, subfamily C, polypeptide 1 )
HSP 1 Score: 325.5 bits (833), Expect = 7.5e-89
Identity = 181/498 (36.35%), Postives = 285/498 (57.23%), Query Frame = 0
Query: 8 VLAVLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQIGENAHVSTTRFAEAYG 67
+L +L FL S LPPGP PI+GN+ +G++ H S ++ YG
Sbjct: 11 LLFCFILSCFLIFTTTRSGRISRGATALPPGPPRLPIIGNIHLVGKHPHRSFAELSKTYG 70
Query: 68 PLISLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPECGE 127
P++SL+LG+ VV +SP AA VL+T D++LSAR + +++ SL++ P
Sbjct: 71 PVMSLKLGSLNTVVIASPEAAREVLRTHDQILSARSPTNAVRSI-NHQDASLVWLPSSSA 130
Query: 128 HWKNLRSLCRANLFSGRAIESQASLRGRKMDEMVEFL--LSNQGSVLEVKDFVFTTVFNT 187
W+ LR L L S + IE+ +LR K+ E+V F+ S++ +++ F T N
Sbjct: 131 RWRLLRRLSVTQLLSPQRIEATKALRMNKVKELVSFISESSDREESVDISRVAFITTLNI 190
Query: 188 LSNLIFSRDLFDF-VGDGFHGIKGSLKKLVSLGLIPNLADFYPLLRNFDIQRLRKKSAIY 247
+SN++FS DL + +G++ ++ ++ P+ A+++P LR D+Q K +
Sbjct: 191 ISNILFSVDLGSYNAKASINGVQDTVISVMDAAGTPDAANYFPFLRFLDLQGNVKTFKVC 250
Query: 248 RKEIDSVWEVLIKER-RELHRQN--ASLPEQDFLDVMI-----HSQFNDQQINFLIFELL 307
+ + V+ I + E QN + + DF+D ++ S+ + I L+ ++
Sbjct: 251 TERLVRVFRGFIDAKIAEKSSQNNPKDVSKNDFVDNLLDYKGDESELSISDIEHLLLDMF 310
Query: 308 MAGADTTTMTVEWAMAELLKNKDTMEKVREEMKTEINDQNIIDESNVSKLHYLWKCVKET 367
AG DT++ T+EWAM ELLKN TM K + E+ I I++ES++SKL YL VKET
Sbjct: 311 TAGTDTSSSTLEWAMTELLKNPKTMAKAQAEIDCVIGQNGIVEESDISKLPYLQAVVKET 370
Query: 368 LRLHPPAPFLLPRVAPMDCELMGYSIPKGTLIFVNVWGIGRDPSIWKDSLAFDPERFISC 427
RLH P P L+PR A D E++G+ + K T + VNVW IGRDPS+W + F+PERF+
Sbjct: 371 FRLHTPVPLLIPRKAESDAEILGFMVLKDTQVLVNVWAIGRDPSVWDNPSQFEPERFLGK 430
Query: 428 DVDFKGYDYRYLPFGGGRRICPGLPMAIVQVTSILATLIHNFEWSLPNGEDFSNLDLNGM 487
D+D +G DY PFG GRRICPG+P+A+ V+ +LA+L+++F+W LP G +LD++
Sbjct: 431 DMDVRGRDYELTPFGAGRRICPGMPLAMKTVSLMLASLLYSFDWKLPKGVLSEDLDMDET 490
Query: 488 LGVTLQKKEPLKLIPKQR 495
G+TL K PL +P ++
Sbjct: 491 FGLTLHKTNPLHAVPVKK 507
BLAST of Spg017434.1 vs. TAIR 10
Match:
AT3G52970.2 (cytochrome P450, family 76, subfamily G, polypeptide 1 )
HSP 1 Score: 323.9 bits (829), Expect = 2.2e-88
Identity = 186/517 (35.98%), Postives = 283/517 (54.74%), Query Frame = 0
Query: 9 LAVLVLVPFLFLLVRYINSPSSKQPPLPPGPKPWPILGNLLQI-GENAHVSTTRFAEAYG 68
+AVL+ V LF R + LPPGP PWP++GN+ Q+ G H S T+ + +G
Sbjct: 17 IAVLIYVTCLFYTKRC-------RTRLPPGPNPWPVIGNIFQLAGLPPHDSLTKLSRRHG 76
Query: 69 PLISLRLGAQLLVVASSPSAAAAVLKTQDRLLSARYIFHMTPDTQQNRECSLLFSPECGE 128
P+++LR+G+ L VV SS A + K D L+ R I+ + + L + + G
Sbjct: 77 PIMTLRIGSMLTVVISSSEVAREIFKKHDAALAGRKIYEAMKGGKSSD--GSLITAQYGA 136
Query: 129 HWKNLRSLCRANLFSGRAIESQASLRGRKMDEMVEFLLSNQGS----------------- 188
+W+ LR LC F R +++ + +R R +D+M+ F+ +
Sbjct: 137 YWRMLRRLCTTQFFVTRRLDAMSDVRSRCVDQMLRFVEEGGQNGMYYTINNIMTCIICTK 196
Query: 189 VLEVKDFVFTTVFNTLSNLIFSRDLFDFVGDGFHGIKGSLKKLVSLGLIPNLADFYPLLR 248
++V + F FN + NL+FSRDL D K++ PN+ADF+PLLR
Sbjct: 197 TIDVGRYFFLMAFNLIGNLMFSRDLLDPDSKRGSEFFYHTGKVMEFAGKPNVADFFPLLR 256
Query: 249 NFDIQRLRKKSAIYRKEIDSVWEVLIKERREL-HRQNASLPEQDFLDVMIH--------- 308
D Q +R+K+ + ++ + I+ER E+ R+ + +D+LDV++
Sbjct: 257 FLDPQGIRRKTQFHVEKAFEIAGEFIRERTEVREREKSDEKTKDYLDVLLEFRGGDGVDE 316
Query: 309 --SQFNDQQINFLIFELLMAGADTTTMTVEWAMAELLKNKDTMEKVREEMKTEINDQN-I 368
S F+ + IN ++FE+ AG DTTT T+EWA+AELL N T+ K++ E++T N
Sbjct: 317 EPSSFSSRDINVIVFEMFTAGTDTTTSTLEWALAELLHNPRTLTKLQTELRTYFKSSNQK 376
Query: 369 IDESNVSKLHYLWKCVKETLRLHPPAPFLLPRVAPMDCELMG-YSIPKGTLIFVNVWGIG 428
+ E ++ L YL + ETLRLHPP PFL+P A C + Y+IPK T + VNVW IG
Sbjct: 377 LQEEDLPNLPYLSAVIMETLRLHPPLPFLVPHKAMSTCHIFDQYTIPKETQVLVNVWAIG 436
Query: 429 RDPSIWKDSLAFDPERFISCD--VDFKGYDYRYLPFGGGRRICPGLPMAIVQVTSILATL 488
RDP W D + F PERFIS DFKG DY +LPFG GRR+CP LP+A + + ++
Sbjct: 437 RDPKTWIDPIMFKPERFISDPDARDFKGQDYEFLPFGSGRRMCPALPLASRVLPLAIGSM 496
Query: 489 IHNFEWSLPNGEDFSNLDLNGMLGVTLQKKEPLKLIP 492
+ +F+W+L NG + +D+ +G+TL+K PL+ IP
Sbjct: 497 VRSFDWALENGLNAEEMDMGERIGITLKKAVPLEAIP 524
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022159319.1 | 9.2e-214 | 74.19 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Momordica charantia] | [more] |
| XP_022928791.1 | 3.0e-204 | 73.35 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata] | [more] |
| XP_038892964.1 | 2.1e-202 | 70.16 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Benincasa hispida] | [more] |
| KAE8647950.1 | 8.1e-202 | 71.57 | hypothetical protein Csa_000545 [Cucumis sativus] | [more] |
| XP_022970204.1 | 1.1e-201 | 72.73 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita maxima] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| O64899 | 1.6e-115 | 43.56 | (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia calif... | [more] |
| Q9FXW4 | 1.6e-115 | 45.77 | Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3... | [more] |
| O64900 | 3.5e-115 | 43.35 | (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3... | [more] |
| Q9SP06 | 1.2e-112 | 43.97 | (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum... | [more] |
| A8CDR5 | 2.3e-103 | 40.00 | Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1E237 | 4.5e-214 | 74.19 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Momordica charantia ... | [more] |
| A0A6J1ELA6 | 1.4e-204 | 73.35 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata O... | [more] |
| A0A6J1I4V7 | 5.1e-202 | 72.73 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=... | [more] |
| A0A5A7UIU3 | 8.7e-202 | 71.84 | Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. ma... | [more] |
| A0A1S3CT14 | 8.7e-202 | 71.84 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT2G45570.1 | 3.9e-93 | 37.30 | cytochrome P450, family 76, subfamily C, polypeptide 2 | [more] |
| AT2G45550.1 | 1.0e-90 | 36.06 | cytochrome P450, family 76, subfamily C, polypeptide 4 | [more] |
| AT3G52970.1 | 1.4e-90 | 37.18 | cytochrome P450, family 76, subfamily G, polypeptide 1 | [more] |
| AT2G45560.1 | 7.5e-89 | 36.35 | cytochrome P450, family 76, subfamily C, polypeptide 1 | [more] |
| AT3G52970.2 | 2.2e-88 | 35.98 | cytochrome P450, family 76, subfamily G, polypeptide 1 | [more] |
