Homology
BLAST of Sed0025890 vs. NCBI nr
Match:
XP_038886229.1 (DIS3-like exonuclease 2 isoform X1 [Benincasa hispida])
HSP 1 Score: 1859.3 bits (4815), Expect = 0.0e+00
Identity = 940/1131 (83.11%), Postives = 1016/1131 (89.83%), Query Frame = 0
Query: 1 MRGAVEQSTPDRNDDGEKEKKKKRRYSRRSKHSASIATPVSCSSVNEISGEASECMENGR 60
MRGAVEQSTPDRN+DG+KEKKKKRR +RRSK +AS++T SC+SVN I+GEASE MENGR
Sbjct: 1 MRGAVEQSTPDRNEDGDKEKKKKRRSNRRSKQNASLSTSASCNSVNGITGEASESMENGR 60
Query: 61 IDANLTAPSNSSSPTQQACQSIHPSEPGLTGRNKISFGSLPPLHISKNAELSELQYSINQ 120
IDANLT+PSN SS TQQA QS HP E GLT RNKI+F SLPPLHIS+ AELSE Q NQ
Sbjct: 61 IDANLTSPSNYSSLTQQAYQSNHPIEHGLTRRNKIAFSSLPPLHISEEAELSESQNLKNQ 120
Query: 121 DLYSLNTGGRIVKSCPEQIACGKMLQLSMSEHSPPADANATVNNTQRKYFTSHWAMDDVN 180
+L+SL+ GGRI+KSCPEQIA G+ +S+++HSPPAD T NN+QRKYF SHW+MDDVN
Sbjct: 121 NLHSLDDGGRIIKSCPEQIAFGRNSGISLNQHSPPAD--VTENNSQRKYFPSHWSMDDVN 180
Query: 181 ERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILINGIASQNRALEGDIVAIMVDPFTS 240
E LQKG IFKALFRVNAHNRLEAYCKIDGLPVD+LINGIASQNRA+EGDIVAI VDPFTS
Sbjct: 181 EGLQKGDIFKALFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIKVDPFTS 240
Query: 241 WTMMKGTCEAHNNKHSMEDANLPAEETEKNSHNCKDKNKVNSDVKSDSYRSSSSSDKRCC 300
WT MKGT EAHNN HSMED NLP E EK+ HNCK KNKV++DVKSDS+RSSS DKRCC
Sbjct: 241 WTRMKGTSEAHNNMHSMEDVNLPFEVAEKDCHNCKGKNKVDADVKSDSFRSSSLPDKRCC 300
Query: 301 SEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHYSSIQDDVSMAIGRICTLINLYPSK 360
SE+KVLDGTACDDLLSNYE CD S V PSQAH+SS QDDVS A+GRIC +INLYP+K
Sbjct: 301 SEDKVLDGTACDDLLSNYEQCDVNQSSVVYPSQAHFSSNQDDVSKAVGRICAVINLYPAK 360
Query: 361 RPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYTKEMKTKSCLSSSHDSGYVQLMPND 420
RPTGRVV I+EKSR RET+VGHLNVKKFLSF EIY KE LS + GYVQL+PND
Sbjct: 361 RPTGRVVTILEKSRLRETVVGHLNVKKFLSFQEIYVKE--NTKFLSPLQNCGYVQLIPND 420
Query: 421 ARFPIMIVLARDLPDCIKKRLENGDVTVESELVAARIDEWVEESSAPRAQVLHVLGQGSK 480
ARFPIM+VLA DLPDCIKKRL+NGD+TVESELVAARI EWV ESSAPRAQVLHVLG+GS+
Sbjct: 421 ARFPIMMVLAEDLPDCIKKRLDNGDLTVESELVAARIHEWVIESSAPRAQVLHVLGRGSE 480
Query: 481 IESHIDAILFENAIRKCEFSQDSLSCLPRTPWKIPHEELQCRRDLRNLCIFTIDPSSVLD 540
+ESHIDAILFENAIR CEFS DSLSC+P TPWKIP EELQCRRD+RNLCIFTIDPSS D
Sbjct: 481 VESHIDAILFENAIRTCEFSHDSLSCIPHTPWKIPQEELQCRRDIRNLCIFTIDPSSASD 540
Query: 541 LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQHKIPMLPPLLS 600
LDDALSVQ LANGIFRVGIH+ADVSHFVLP TALDKEAQIRS SVYLLQ KIPMLPPLLS
Sbjct: 541 LDDALSVQILANGIFRVGIHVADVSHFVLPGTALDKEAQIRSMSVYLLQRKIPMLPPLLS 600
Query: 601 ENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSCCKLSYEHAQDIIDGLIDSDS 660
EN+ SLNPGVDRLAFSLFLD+++CG VK+ WI RTVICSCCKLSYE AQDIIDGLIDSDS
Sbjct: 601 ENIGSLNPGVDRLAFSLFLDINNCGDVKECWIGRTVICSCCKLSYEQAQDIIDGLIDSDS 660
Query: 661 SKIL-GNCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRDGALRLENSKIVYLYDEYGI 720
SKIL NCPQLH QF+W DVISSVK+LHEISKTLKEKRFRDGALRLENSKI++LYDE GI
Sbjct: 661 SKILRNNCPQLHGQFAWHDVISSVKLLHEISKTLKEKRFRDGALRLENSKIIFLYDECGI 720
Query: 721 PYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTYPNSALLRRQPEPILRKLREFESFCS 780
PYDSTFYEQKDSNFLVEEFMLLAN TVAEVISRT+P+SALLRR P+PILRKLREFESFCS
Sbjct: 721 PYDSTFYEQKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPDPILRKLREFESFCS 780
Query: 781 KHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYATRPMQLATYFCNGELKDGENG 840
+HG ELDTSSSV+FQQSLEQIRIKLHDDP LF+I+ SYATRPMQLATYFC+GELKDGE G
Sbjct: 781 RHGFELDTSSSVQFQQSLEQIRIKLHDDPLLFDILTSYATRPMQLATYFCSGELKDGEKG 840
Query: 841 SHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEAEELYLKHQGIIPKVNSDEQMRCFTG 900
SHYALA PLYTHFTSPLRRYPDIVVHRTLAAA+EAE LYLKH+GII KVNSDEQMRCFTG
Sbjct: 841 SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEMLYLKHRGIIQKVNSDEQMRCFTG 900
Query: 901 IYFDKDAADSSEGREALSSAASRHGLPCSKLLSDVALHCNNRKLASKHVADACNKLYMWA 960
I FDKDAADS EGREALSSAA RHG+PCSKLLSDVA+HCNNRKLASKHVAD C KLYMWA
Sbjct: 901 ISFDKDAADSLEGREALSSAALRHGVPCSKLLSDVAVHCNNRKLASKHVADGCEKLYMWA 960
Query: 961 LLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLEVEWLDATSTLVLSFFG 1020
LLK+KK+LFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGL VEWLD TSTLVLSFFG
Sbjct: 961 LLKKKKILFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLAVEWLDTTSTLVLSFFG 1020
Query: 1021 TRRPHRNRGSSIKWKALEDVALVISPCDLNVKQRTLGGMSLSESGGAGTGGASVEQEPNL 1080
TRR HRNRG SIKWKALEDVAL+ISPCD N+KQRTL G+S SE GGA TGG++VEQE NL
Sbjct: 1021 TRRSHRNRG-SIKWKALEDVALIISPCDQNIKQRTL-GVSPSELGGATTGGSAVEQESNL 1080
Query: 1081 KSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYTSSYLK 1131
KSHVSDTG+DPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLY SSYL+
Sbjct: 1081 KSHVSDTGVDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYMSSYLR 1125
BLAST of Sed0025890 vs. NCBI nr
Match:
KAG7016503.1 (DIS3-like exonuclease 2 [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1851.6 bits (4795), Expect = 0.0e+00
Identity = 931/1131 (82.32%), Postives = 1015/1131 (89.74%), Query Frame = 0
Query: 1 MRGAVEQSTPDRNDDGEKEKKKKRRYSRRSKHSASIATPVSCSSVNEISGEASECMENGR 60
MRGAVEQSTP+R DDG+KEKKKKRR +RRSK +ASI+T VSCSSVN +SGEASECMENG+
Sbjct: 1 MRGAVEQSTPERYDDGDKEKKKKRRSNRRSKQNASISTSVSCSSVNGMSGEASECMENGK 60
Query: 61 IDANLTAPSNSSSPTQQACQSIHPSEPGLTGRNKISFGSLPPLHISKNAELSELQYSINQ 120
IDANLTAPSN SS TQQA +S HP E G+T RNKI+F SLPPLHIS+ AELSE Q IN+
Sbjct: 61 IDANLTAPSNHSSLTQQAHESNHPIEHGVTRRNKIAFSSLPPLHISEQAELSESQNLINE 120
Query: 121 DLYSLNTGGRIVKSCPEQIACGKMLQLSMSEHSPPADANATVNNTQRKYFTSHWAMDDVN 180
+L+ L+ GG+ +KSCPEQI CG+M +S+++HSPPAD T NNTQRKYF SHW+++DV+
Sbjct: 121 NLHPLDAGGKTIKSCPEQIVCGRMPGISINQHSPPAD--VTENNTQRKYFASHWSVEDVD 180
Query: 181 ERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILINGIASQNRALEGDIVAIMVDPFTS 240
E LQKG IF+A FRVNAHNRLEAYCKIDGLPVD+LINGIASQNRA+EGDIVAIMVDP TS
Sbjct: 181 EGLQKGDIFRAFFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIMVDPLTS 240
Query: 241 WTMMKGTCEAHNNKHSMEDANLPAEETEKNSHNCKDKNKVNSDVKSDSYRSSSSSDKRCC 300
W MKGT E HN+ HSMEDANLPAE TE + NCK KNK+++ VKSDS+RSSSS DKRCC
Sbjct: 241 WIRMKGTSETHNSTHSMEDANLPAEATENDGRNCKGKNKLDASVKSDSFRSSSSPDKRCC 300
Query: 301 SEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHYSSIQDDVSMAIGRICTLINLYPSK 360
SE+K+LDGTACDDLL E D Y S V P +AHYSS QDDVS AI RIC +I+ YP K
Sbjct: 301 SEDKILDGTACDDLLLKNEQRDVYQSSVVDPPEAHYSSNQDDVSKAIQRICAVISSYPGK 360
Query: 361 RPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYTKEMKTKSCLSSSHDSGYVQLMPND 420
RPTGRVVAI+EKSR RE+IVGHLNVKKFLSF EIY KEM TKSCLS SH+ G+VQLMPND
Sbjct: 361 RPTGRVVAILEKSRQRESIVGHLNVKKFLSFQEIYMKEMNTKSCLSPSHNCGHVQLMPND 420
Query: 421 ARFPIMIVLARDLPDCIKKRLENGDVTVESELVAARIDEWVEESSAPRAQVLHVLGQGSK 480
ARFPIM+VLA DLPD IKKRL+NGDVTVE+ELVA +I EWV+ESSAP+A VLHVLG+GS+
Sbjct: 421 ARFPIMMVLAGDLPDSIKKRLDNGDVTVENELVAVKIHEWVKESSAPQAHVLHVLGRGSE 480
Query: 481 IESHIDAILFENAIRKCEFSQDSLSCLPRTPWKIPHEELQCRRDLRNLCIFTIDPSSVLD 540
+ SHIDAILFENAI CEFS DSL+CLP +PWKIPHEELQCRRDLRNLCIFTIDPSS D
Sbjct: 481 VASHIDAILFENAIHSCEFSNDSLACLPHSPWKIPHEELQCRRDLRNLCIFTIDPSSASD 540
Query: 541 LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQHKIPMLPPLLS 600
LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQ KIPMLPPLLS
Sbjct: 541 LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS 600
Query: 601 ENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSCCKLSYEHAQDIIDGLIDSDS 660
EN+ SL+PGVDRLAFSLFLD+D+CG VKD WI RTVICSCCKLSYEHAQDIIDGLIDSDS
Sbjct: 601 ENVGSLSPGVDRLAFSLFLDIDNCGDVKDRWIGRTVICSCCKLSYEHAQDIIDGLIDSDS 660
Query: 661 SKILG-NCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRDGALRLENSKIVYLYDEYGI 720
SK LG N PQLH QF+W DVISSVK+LHEISKTLK+KRFRDGALRLENSKIVYLYDEYG+
Sbjct: 661 SKNLGNNYPQLHGQFAWPDVISSVKLLHEISKTLKKKRFRDGALRLENSKIVYLYDEYGV 720
Query: 721 PYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTYPNSALLRRQPEPILRKLREFESFCS 780
PYDSTFYEQKDSNFLVEEFMLLAN TVAEVISRT+P+SALLRR PEPILRKLREFESFCS
Sbjct: 721 PYDSTFYEQKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPILRKLREFESFCS 780
Query: 781 KHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYATRPMQLATYFCNGELKDGENG 840
KHG ELDTSSSV+FQQSLEQIRIKLHDDP LF+I+ SYATRPMQLATYFC+GELKDGE G
Sbjct: 781 KHGFELDTSSSVQFQQSLEQIRIKLHDDPLLFDILTSYATRPMQLATYFCSGELKDGEKG 840
Query: 841 SHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEAEELYLKHQGIIPKVNSDEQMRCFTG 900
SHYALA PLYTHFTSPLRRYPDIVVHRTLAAA+EAE+LYLKH+GII KVNSDEQ+RCFTG
Sbjct: 841 SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKLYLKHRGIIQKVNSDEQIRCFTG 900
Query: 901 IYFDKDAADSSEGREALSSAASRHGLPCSKLLSDVALHCNNRKLASKHVADACNKLYMWA 960
+YFDKDAADS EGREALSSAA RHG+PC+KLL+DVALHCNNRKLASKHVAD C+KLYMWA
Sbjct: 901 MYFDKDAADSLEGREALSSAALRHGVPCAKLLADVALHCNNRKLASKHVADGCDKLYMWA 960
Query: 961 LLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLEVEWLDATSTLVLSFFG 1020
LLK+KKVLFSDARVLGLGPRFMS+YIQKL IERRIYYDE EGL VEWL+ TSTLVLSFFG
Sbjct: 961 LLKKKKVLFSDARVLGLGPRFMSLYIQKLDIERRIYYDETEGLAVEWLETTSTLVLSFFG 1020
Query: 1021 TRRPHRNRGSSIKWKALEDVALVISPCDLNVKQRTLGGMSLSESGGAGTGGASVEQEPNL 1080
TRR HR+RG SIKWKALEDVALV+SPCD NVKQR L G+S SE GG TGGA VEQE NL
Sbjct: 1021 TRRSHRSRG-SIKWKALEDVALVVSPCDHNVKQRAL-GVSPSELGGTSTGGAVVEQESNL 1080
Query: 1081 KSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYTSSYLK 1131
KSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLY SSYLK
Sbjct: 1081 KSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYMSSYLK 1127
BLAST of Sed0025890 vs. NCBI nr
Match:
XP_023550262.1 (DIS3-like exonuclease 2 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1850.1 bits (4791), Expect = 0.0e+00
Identity = 930/1131 (82.23%), Postives = 1016/1131 (89.83%), Query Frame = 0
Query: 1 MRGAVEQSTPDRNDDGEKEKKKKRRYSRRSKHSASIATPVSCSSVNEISGEASECMENGR 60
MRGAVEQSTP+R DDG+KEKKKKRR +RRSK +ASI+T VSCSSVN +SGEASECMENG+
Sbjct: 1 MRGAVEQSTPERYDDGDKEKKKKRRSNRRSKQNASISTSVSCSSVNGMSGEASECMENGK 60
Query: 61 IDANLTAPSNSSSPTQQACQSIHPSEPGLTGRNKISFGSLPPLHISKNAELSELQYSINQ 120
IDANLTAPSN SS TQQA +S HP E G+T RNKI+F SLPPLHIS+ AELSE Q IN+
Sbjct: 61 IDANLTAPSNHSSLTQQAHESNHPIEHGVTRRNKIAFSSLPPLHISEEAELSESQNLINE 120
Query: 121 DLYSLNTGGRIVKSCPEQIACGKMLQLSMSEHSPPADANATVNNTQRKYFTSHWAMDDVN 180
+L+ L+ GG+I+KSCPEQI CG+M +S+++HSPPAD T NNTQRKYF SHW+++DV+
Sbjct: 121 NLHPLDAGGKIIKSCPEQIVCGRMPGISINQHSPPAD--VTENNTQRKYFASHWSVEDVD 180
Query: 181 ERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILINGIASQNRALEGDIVAIMVDPFTS 240
E LQKG IF+ALFRVNAHNRLEAYCKIDGLPVD+LINGIASQNRA+EGDIVAIMVDP TS
Sbjct: 181 EGLQKGDIFRALFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIMVDPLTS 240
Query: 241 WTMMKGTCEAHNNKHSMEDANLPAEETEKNSHNCKDKNKVNSDVKSDSYRSSSSSDKRCC 300
WT MKGT EAHN HS+EDANLP E TE + NCK KNKV++ VKSDS+RSSSS DKRCC
Sbjct: 241 WTRMKGTSEAHNCTHSVEDANLPVEATENDGRNCKGKNKVDASVKSDSFRSSSSPDKRCC 300
Query: 301 SEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHYSSIQDDVSMAIGRICTLINLYPSK 360
SE+K+LDGTAC D+L E D Y S V P +AHYSS QDDVS AI RIC +I+ YP K
Sbjct: 301 SEDKILDGTACGDVLLKNEQRDVYQSSVVDPPEAHYSSNQDDVSKAIQRICAVISSYPGK 360
Query: 361 RPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYTKEMKTKSCLSSSHDSGYVQLMPND 420
RPTGRVVAI+EK+R RE+IVGHLNVKKFLSF EIY KEM TKSCLS SH+ GYVQLMPND
Sbjct: 361 RPTGRVVAILEKTRQRESIVGHLNVKKFLSFQEIYMKEMNTKSCLSPSHNCGYVQLMPND 420
Query: 421 ARFPIMIVLARDLPDCIKKRLENGDVTVESELVAARIDEWVEESSAPRAQVLHVLGQGSK 480
ARFP M+VLA DLPD IKKRL+NGDVTVE+ELVA +I EWV ESSAP+A VLHVLG+GS+
Sbjct: 421 ARFPTMMVLAGDLPDSIKKRLDNGDVTVENELVAVKIHEWVNESSAPQAHVLHVLGRGSE 480
Query: 481 IESHIDAILFENAIRKCEFSQDSLSCLPRTPWKIPHEELQCRRDLRNLCIFTIDPSSVLD 540
+ SHIDAILFENAI CEFS DSL+CLP TPWKIP EELQCRRDLRNLC+FTIDPSS D
Sbjct: 481 VASHIDAILFENAIHSCEFSNDSLACLPHTPWKIPPEELQCRRDLRNLCVFTIDPSSASD 540
Query: 541 LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQHKIPMLPPLLS 600
LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQ KIPMLPPLLS
Sbjct: 541 LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS 600
Query: 601 ENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSCCKLSYEHAQDIIDGLIDSDS 660
EN+ SL+PGVDRLAFSLFLD+D+CG VKD WI RTVICSCCKLSYEHAQDIIDGLIDSDS
Sbjct: 601 ENVGSLSPGVDRLAFSLFLDIDNCGDVKDRWIGRTVICSCCKLSYEHAQDIIDGLIDSDS 660
Query: 661 SKILG-NCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRDGALRLENSKIVYLYDEYGI 720
SK LG N PQLH QF+W DVISSVK+LHEISKTLK+KRFRDGALRLENSKIVYLYDEYGI
Sbjct: 661 SKNLGNNYPQLHGQFAWPDVISSVKLLHEISKTLKKKRFRDGALRLENSKIVYLYDEYGI 720
Query: 721 PYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTYPNSALLRRQPEPILRKLREFESFCS 780
PYDSTFYEQKDSNFLVEEFMLLAN +VAEVISRT+P+SALLRR PEPILRKLREFESFCS
Sbjct: 721 PYDSTFYEQKDSNFLVEEFMLLANTSVAEVISRTFPDSALLRRHPEPILRKLREFESFCS 780
Query: 781 KHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYATRPMQLATYFCNGELKDGENG 840
KHG ELDTSSSV+FQQSLEQIRIKLHDDP LF+I+ SYATRPMQLATYFC+GELKDGE G
Sbjct: 781 KHGFELDTSSSVQFQQSLEQIRIKLHDDPLLFDILTSYATRPMQLATYFCSGELKDGEKG 840
Query: 841 SHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEAEELYLKHQGIIPKVNSDEQMRCFTG 900
SHYALA PLYTHFTSPLRRYPDIVVHRTLAAA+EAE+LYLKH+G+I KVNSDEQ+RCFTG
Sbjct: 841 SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKLYLKHRGMIQKVNSDEQIRCFTG 900
Query: 901 IYFDKDAADSSEGREALSSAASRHGLPCSKLLSDVALHCNNRKLASKHVADACNKLYMWA 960
+YFDKDAADS EGREALSSAA RHG+PC+KLL+DVALHCNNRKLASKHVAD C+KLYMWA
Sbjct: 901 MYFDKDAADSLEGREALSSAALRHGVPCAKLLADVALHCNNRKLASKHVADGCDKLYMWA 960
Query: 961 LLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLEVEWLDATSTLVLSFFG 1020
LLK+KKVLFSDARVLGLGPRFMS+YIQKLAIERR+YYDE EGL VEWL+ TSTLVLSFFG
Sbjct: 961 LLKKKKVLFSDARVLGLGPRFMSLYIQKLAIERRVYYDETEGLAVEWLETTSTLVLSFFG 1020
Query: 1021 TRRPHRNRGSSIKWKALEDVALVISPCDLNVKQRTLGGMSLSESGGAGTGGASVEQEPNL 1080
TRR HR+RG SIKWKALEDVALV+SPCD NVKQRTL G+S SE GG GTGGA VEQE NL
Sbjct: 1021 TRRSHRSRG-SIKWKALEDVALVVSPCDQNVKQRTL-GVSPSELGGTGTGGAVVEQESNL 1080
Query: 1081 KSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYTSSYLK 1131
KSHVSDTGI+PAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLY SSYLK
Sbjct: 1081 KSHVSDTGIEPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYMSSYLK 1127
BLAST of Sed0025890 vs. NCBI nr
Match:
XP_022992945.1 (DIS3-like exonuclease 2 [Cucurbita maxima])
HSP 1 Score: 1848.9 bits (4788), Expect = 0.0e+00
Identity = 930/1131 (82.23%), Postives = 1011/1131 (89.39%), Query Frame = 0
Query: 1 MRGAVEQSTPDRNDDGEKEKKKKRRYSRRSKHSASIATPVSCSSVNEISGEASECMENGR 60
MRGAVEQSTP+R DDG+KEKKKKRR +RRSK +ASI+T VSCSSVN +SGEASECMENG+
Sbjct: 1 MRGAVEQSTPERYDDGDKEKKKKRRSNRRSKQNASISTSVSCSSVNGMSGEASECMENGK 60
Query: 61 IDANLTAPSNSSSPTQQACQSIHPSEPGLTGRNKISFGSLPPLHISKNAELSELQYSINQ 120
IDANLTAPSN SS TQQA +S HP E G+T RNKI+F SLPPLHIS+ AELSE Q IN+
Sbjct: 61 IDANLTAPSNHSSLTQQAHESNHPIEHGVTRRNKIAFSSLPPLHISEQAELSESQNLINE 120
Query: 121 DLYSLNTGGRIVKSCPEQIACGKMLQLSMSEHSPPADANATVNNTQRKYFTSHWAMDDVN 180
+L+ L+ GG+ +KSCPEQI CG+M +S ++HS PAD T NN+QRKYF SHW+++DV+
Sbjct: 121 NLHPLDAGGKTIKSCPEQIVCGRMPGISTNQHSSPAD--VTENNSQRKYFASHWSVEDVD 180
Query: 181 ERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILINGIASQNRALEGDIVAIMVDPFTS 240
E LQKG IF+A FRVNAHNRLEAYCKIDGLPVD+LINGIASQNRA+EGDIVAIMVDP TS
Sbjct: 181 EGLQKGDIFRAFFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIMVDPLTS 240
Query: 241 WTMMKGTCEAHNNKHSMEDANLPAEETEKNSHNCKDKNKVNSDVKSDSYRSSSSSDKRCC 300
WT MKGT E HN+ HSMEDANLPAE TE + NCK KNKV++ VKSDS+RSSSS DKRCC
Sbjct: 241 WTRMKGTSETHNSTHSMEDANLPAEATENDGRNCKGKNKVDASVKSDSFRSSSSPDKRCC 300
Query: 301 SEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHYSSIQDDVSMAIGRICTLINLYPSK 360
SE+K+LDGTACDDLL E D Y V +AHYSS QDDVS AI RIC +I+ YP K
Sbjct: 301 SEDKILDGTACDDLLLKNEQRDVYQSLVVDLPEAHYSSNQDDVSKAIQRICAVISSYPGK 360
Query: 361 RPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYTKEMKTKSCLSSSHDSGYVQLMPND 420
RPTGRVVAI+EKSR RE+IVGHLNVKKFLSF EIY KEM TKSCLS SH+ GYVQLMPND
Sbjct: 361 RPTGRVVAILEKSRQRESIVGHLNVKKFLSFQEIYMKEMNTKSCLSPSHNCGYVQLMPND 420
Query: 421 ARFPIMIVLARDLPDCIKKRLENGDVTVESELVAARIDEWVEESSAPRAQVLHVLGQGSK 480
ARFPIM+VLA DLPD IKKRL+NGDVTVE+ELVA +I EWV+ESSAP+A VLHVLG+GS+
Sbjct: 421 ARFPIMVVLAGDLPDSIKKRLDNGDVTVENELVAVKIHEWVKESSAPQAHVLHVLGRGSE 480
Query: 481 IESHIDAILFENAIRKCEFSQDSLSCLPRTPWKIPHEELQCRRDLRNLCIFTIDPSSVLD 540
+ SHIDAILFENAI CEFS DSL+CLP TPWKIPHEELQCRRDLRNLCIFTIDPSS D
Sbjct: 481 VASHIDAILFENAIHSCEFSNDSLACLPHTPWKIPHEELQCRRDLRNLCIFTIDPSSASD 540
Query: 541 LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQHKIPMLPPLLS 600
LDDALSVQKLANGIFRVG+HIADVSHFVLPDTALDKEAQIRSTSVYLLQ KIPMLPPLLS
Sbjct: 541 LDDALSVQKLANGIFRVGVHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS 600
Query: 601 ENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSCCKLSYEHAQDIIDGLIDSDS 660
EN+ SL+PGVDRLAFSLFLD+D+CG VKD WI RTVICSCCKLSYEHAQDIIDGLIDSDS
Sbjct: 601 ENVGSLSPGVDRLAFSLFLDIDNCGDVKDRWIGRTVICSCCKLSYEHAQDIIDGLIDSDS 660
Query: 661 SKILG-NCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRDGALRLENSKIVYLYDEYGI 720
SK LG N PQLH QF WLDVISSVKILHEISKTLK+KRFRDGALRLENSKIVYLYDEYGI
Sbjct: 661 SKNLGNNYPQLHGQFEWLDVISSVKILHEISKTLKKKRFRDGALRLENSKIVYLYDEYGI 720
Query: 721 PYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTYPNSALLRRQPEPILRKLREFESFCS 780
PYDS FYEQKDSNFLVEEFMLLAN TVAEVISRT+P+SALLRR PEPILRKLREFESFCS
Sbjct: 721 PYDSAFYEQKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPILRKLREFESFCS 780
Query: 781 KHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYATRPMQLATYFCNGELKDGENG 840
KHG ELDTSSSV+FQQSLEQIRIKLHDDP LF+I+ SYATRPMQLATYFC+GELKDGE G
Sbjct: 781 KHGFELDTSSSVQFQQSLEQIRIKLHDDPLLFDILTSYATRPMQLATYFCSGELKDGEKG 840
Query: 841 SHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEAEELYLKHQGIIPKVNSDEQMRCFTG 900
SHYALA PLYTHFTSPLRRYPDI+VHRTLAAA+EAE+LYLKH+GI KVNSDEQ+RCFTG
Sbjct: 841 SHYALAVPLYTHFTSPLRRYPDIIVHRTLAAAIEAEKLYLKHRGITQKVNSDEQIRCFTG 900
Query: 901 IYFDKDAADSSEGREALSSAASRHGLPCSKLLSDVALHCNNRKLASKHVADACNKLYMWA 960
+YFDKDAADS EG+EALSSAA RHG+PC+KLL+DVALHCNNRKLASKHVAD C+KLYMWA
Sbjct: 901 MYFDKDAADSLEGKEALSSAALRHGVPCAKLLADVALHCNNRKLASKHVADGCDKLYMWA 960
Query: 961 LLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLEVEWLDATSTLVLSFFG 1020
LLK+KKVLFSDARVLGLGPRFMS+YIQKLAIERRIYYDE EGL VEWL+ TSTLVLSFFG
Sbjct: 961 LLKKKKVLFSDARVLGLGPRFMSLYIQKLAIERRIYYDETEGLAVEWLETTSTLVLSFFG 1020
Query: 1021 TRRPHRNRGSSIKWKALEDVALVISPCDLNVKQRTLGGMSLSESGGAGTGGASVEQEPNL 1080
TRR HR+RG SIKWKALEDVALV+SPCD NVKQR LG S SE GG GTGGA VEQE NL
Sbjct: 1021 TRRSHRSRG-SIKWKALEDVALVVSPCDQNVKQRALGA-SPSELGGTGTGGAVVEQESNL 1080
Query: 1081 KSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYTSSYLK 1131
KSHVSDTGIDPAVFPLTVRLLST+PVALHAVGGDDGPIDIGVRLY SSYLK
Sbjct: 1081 KSHVSDTGIDPAVFPLTVRLLSTLPVALHAVGGDDGPIDIGVRLYMSSYLK 1127
BLAST of Sed0025890 vs. NCBI nr
Match:
XP_022938380.1 (DIS3-like exonuclease 2 [Cucurbita moschata])
HSP 1 Score: 1840.5 bits (4766), Expect = 0.0e+00
Identity = 926/1131 (81.87%), Postives = 1013/1131 (89.57%), Query Frame = 0
Query: 1 MRGAVEQSTPDRNDDGEKEKKKKRRYSRRSKHSASIATPVSCSSVNEISGEASECMENGR 60
MRGAVEQSTP+R DDG+KEKKKKRR +RRSK +ASI+T VSCSSVN + GEASEC ENG+
Sbjct: 1 MRGAVEQSTPERYDDGDKEKKKKRRSNRRSKQNASISTSVSCSSVNGMPGEASECRENGK 60
Query: 61 IDANLTAPSNSSSPTQQACQSIHPSEPGLTGRNKISFGSLPPLHISKNAELSELQYSINQ 120
I+ANLTAPSN SS TQQA +S HP E G+T RNKI+F SLPPLHIS+ AELSE Q IN+
Sbjct: 61 INANLTAPSNHSSLTQQAHESNHPIEHGVTRRNKIAFSSLPPLHISEQAELSESQNLINE 120
Query: 121 DLYSLNTGGRIVKSCPEQIACGKMLQLSMSEHSPPADANATVNNTQRKYFTSHWAMDDVN 180
+L+ L+ GG+ +KSCPEQI CG+M +S+++HSPPAD T NNTQRKYF SHW+++DV+
Sbjct: 121 NLHPLDAGGKTIKSCPEQIVCGRMPGISINQHSPPAD--VTENNTQRKYFASHWSVEDVD 180
Query: 181 ERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILINGIASQNRALEGDIVAIMVDPFTS 240
E LQKG IF+A FRVN+HNRLEAYCKIDGLPVD+LINGIASQNRA+EGDIVAIMVDP TS
Sbjct: 181 EGLQKGDIFRAFFRVNSHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIMVDPLTS 240
Query: 241 WTMMKGTCEAHNNKHSMEDANLPAEETEKNSHNCKDKNKVNSDVKSDSYRSSSSSDKRCC 300
WT MKGT E HN+ HSMEDANLPAE TE + NCK KNK+++ VKSDS+RSSSS DKRCC
Sbjct: 241 WTRMKGTSETHNSTHSMEDANLPAEATENDGRNCKGKNKLDASVKSDSFRSSSSPDKRCC 300
Query: 301 SEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHYSSIQDDVSMAIGRICTLINLYPSK 360
SE+K+LDGTACDDLL E D Y S V P +AHYS QDDVS AI RIC +I+ YP K
Sbjct: 301 SEDKILDGTACDDLLLKNEQRDVYQSSVVDPPEAHYSRNQDDVSKAIQRICAVISSYPGK 360
Query: 361 RPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYTKEMKTKSCLSSSHDSGYVQLMPND 420
RPTGRVVAI+EKSR RE+IVGHLNVKKFLSF EIY KEM TKSCLS SH+ GYVQLMPND
Sbjct: 361 RPTGRVVAILEKSRQRESIVGHLNVKKFLSFQEIYMKEMNTKSCLSPSHNCGYVQLMPND 420
Query: 421 ARFPIMIVLARDLPDCIKKRLENGDVTVESELVAARIDEWVEESSAPRAQVLHVLGQGSK 480
ARFPIM+VLA DLPD IKKRL+NGDVTVE+ELVA +I EWV+ESSAP+A VLHVLG+GS+
Sbjct: 421 ARFPIMMVLAGDLPDSIKKRLDNGDVTVENELVAVKIHEWVKESSAPQALVLHVLGRGSE 480
Query: 481 IESHIDAILFENAIRKCEFSQDSLSCLPRTPWKIPHEELQCRRDLRNLCIFTIDPSSVLD 540
+ SHIDAILFENAI CEFS DSL+CLP TPWKIPHEELQCRRDLRNLCIFTIDPSS D
Sbjct: 481 VASHIDAILFENAIHSCEFSNDSLACLPHTPWKIPHEELQCRRDLRNLCIFTIDPSSASD 540
Query: 541 LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQHKIPMLPPLLS 600
LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQ KIPMLPPLLS
Sbjct: 541 LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS 600
Query: 601 ENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSCCKLSYEHAQDIIDGLIDSDS 660
+N+ SL+PGVDRLAFSLFLD+D+ G VKD WI RTVICSCCKLSYEHAQDIIDGLIDSD+
Sbjct: 601 KNVGSLSPGVDRLAFSLFLDIDNSGDVKDRWIGRTVICSCCKLSYEHAQDIIDGLIDSDN 660
Query: 661 SKILG-NCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRDGALRLENSKIVYLYDEYGI 720
K LG N PQLH QF+W DVISSVK+LHEISKTLK+KRFRDGALRLENSKIVYLYDEYGI
Sbjct: 661 LKNLGNNYPQLHGQFAWPDVISSVKLLHEISKTLKKKRFRDGALRLENSKIVYLYDEYGI 720
Query: 721 PYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTYPNSALLRRQPEPILRKLREFESFCS 780
PYDSTFYEQKDSNFLVEEFMLLAN TVAEVISRT+P+SALLRR PEPILRKLREFESFCS
Sbjct: 721 PYDSTFYEQKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPILRKLREFESFCS 780
Query: 781 KHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYATRPMQLATYFCNGELKDGENG 840
KHG ELDTSSSV+FQQSLEQIRIKLHDDP LF+I+ SYATRPMQLATYFC+GELKDGE G
Sbjct: 781 KHGFELDTSSSVQFQQSLEQIRIKLHDDPLLFDILTSYATRPMQLATYFCSGELKDGEKG 840
Query: 841 SHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEAEELYLKHQGIIPKVNSDEQMRCFTG 900
SHYALA PLYTHFTSPLRRYPDIVVHRTLAAA+EAE+LYLKH+GII KVNSDEQ+RCFTG
Sbjct: 841 SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKLYLKHRGIIQKVNSDEQIRCFTG 900
Query: 901 IYFDKDAADSSEGREALSSAASRHGLPCSKLLSDVALHCNNRKLASKHVADACNKLYMWA 960
+YFDKDAADS EGREALSSAA RHG+PC+KLL+DVALHCN+RKLASKHVAD C+KLYMWA
Sbjct: 901 MYFDKDAADSLEGREALSSAALRHGVPCAKLLADVALHCNDRKLASKHVADGCDKLYMWA 960
Query: 961 LLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLEVEWLDATSTLVLSFFG 1020
+LK+KKVLFSDARVLGLGPRFMS+YIQKLAIERRIYYDE EGL VEWL+ TSTLVLSFFG
Sbjct: 961 VLKKKKVLFSDARVLGLGPRFMSLYIQKLAIERRIYYDETEGLAVEWLETTSTLVLSFFG 1020
Query: 1021 TRRPHRNRGSSIKWKALEDVALVISPCDLNVKQRTLGGMSLSESGGAGTGGASVEQEPNL 1080
TRR HR+RG SIKWKALEDVALV+SPCD NVKQR L G+S SE GG GTGGA VEQE NL
Sbjct: 1021 TRRSHRSRG-SIKWKALEDVALVVSPCDHNVKQRAL-GVSPSELGGTGTGGAVVEQESNL 1080
Query: 1081 KSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYTSSYLK 1131
KSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLY SSYLK
Sbjct: 1081 KSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYMSSYLK 1127
BLAST of Sed0025890 vs. ExPASy Swiss-Prot
Match:
P0DM58 (DIS3-like exonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=SOV PE=1 SV=1)
HSP 1 Score: 962.6 bits (2487), Expect = 3.9e-279
Identity = 553/1134 (48.77%), Postives = 726/1134 (64.02%), Query Frame = 0
Query: 1 MRGAVEQSTPDRNDDGEKEKKKK-RRYSRRSKHSASIATPVSCSSVNEISGEASECMENG 60
M+ A + + +R ++G K+K+ + ++ +RRSK S + P+ + V E +G
Sbjct: 1 MKSASSEQSVERIENGHKKKRNRPQKQNRRSKQS---SVPIEDAHVEE--------SLDG 60
Query: 61 RIDANLTAPSNSSSPTQQACQSIHPSEPGLTGRNKISFGSLPPLHISKNAELSELQYSIN 120
R + A ++SS QQ + E + ++F S+PP+ AE + S +
Sbjct: 61 RDSSRSKAKDSTSSSKQQRPNT---DELEAMRASNVAFNSMPPM----RAESGYPRRSAS 120
Query: 121 QDLYSLNTGGRIV-KSCPEQIACGKMLQLSMSEHSPPADA---NATVNNTQRKYFTSHWA 180
L S +++ KSCP+ AC E SP + ++QRK F+SHW+
Sbjct: 121 PLLSSPEVSKQLLSKSCPDPRAC---------EQSPGMNGELFQQIEGSSQRKIFSSHWS 180
Query: 181 MDDVNERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILINGIASQNRALEGDIVAIMV 240
+D V E L+KG FKALFRVNAHNR EAYCKIDG+P DILING Q+RA+EGD V I +
Sbjct: 181 LDAVTEALEKGEAFKALFRVNAHNRNEAYCKIDGVPTDILINGNVCQSRAVEGDTVVIKL 240
Query: 241 DPFTSWTMMKGTCEAHNNKHSMEDANLPAEETEKNSHNCKDKNKVNSDVKSDSYRSSSSS 300
DP + W MKG K E N P E+ +K + + V S SS
Sbjct: 241 DPLSLWPKMKGFVTESAAK--PEGTNSPPEKDDKKARQKNGIDVVEGFEDGFSKNKSSVI 300
Query: 301 DKRCCSEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHYSSIQDDVSMAIGRICTLIN 360
K + + D L + +C+ Q A+ ++C +++
Sbjct: 301 GKGAKNGVTPSSPPSLDSCLGS--FCE-----------------QKGNCSAVDKLCGILS 360
Query: 361 LYPSKRPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYTKEMKTKSCLSSSHDSGYVQ 420
+P KRPTG+VVA++EKS R++IVG L+VK ++ + E K K+ LS D YVQ
Sbjct: 361 SFPHKRPTGQVVAVVEKSLVRDSIVGLLDVKGWIHYKESDPKRCKSPLSLS---DDEYVQ 420
Query: 421 LMPNDARFPIMIVLARDLPDCIKKRLENGDVTVESELVAARIDEWVEESSAPRAQVLHVL 480
LMP D RFP +IV LP I+ RLEN D +E+ELVAA+I +W E S P AQ+ H+
Sbjct: 421 LMPADPRFPKLIVPFHVLPGSIRARLENLDPNLEAELVAAQIVDWGEGSPFPVAQITHLF 480
Query: 481 GQGSKIESHIDAILFENAIRKCEFSQDSLSCLPRTPWKIPHEELQCRRDLRNLCIFTIDP 540
G+GS++E I+AIL++N++ +FS SL+ LPR PW++P EE+Q R+DLR+LC+ TIDP
Sbjct: 481 GRGSELEPQINAILYQNSVCDSDFSPGSLTSLPRVPWEVPEEEVQRRKDLRDLCVLTIDP 540
Query: 541 SSVLDLDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQHKIPML 600
S+ DLDDALSVQ L G FRVG+HIADVS+FVLP+TALD EA+ RSTSVYL+Q KI ML
Sbjct: 541 STATDLDDALSVQSLPGGFFRVGVHIADVSYFVLPETALDTEARFRSTSVYLMQRKISML 600
Query: 601 PPLLSENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSCCKLSYEHAQDIIDGL 660
PPLLSEN+ SL+PG DRLAFS+ DL+ G V D WI RT+I SCCKLSY+HAQDIIDG
Sbjct: 601 PPLLSENVGSLSPGADRLAFSILWDLNREGDVIDRWIGRTIIRSCCKLSYDHAQDIIDGK 660
Query: 661 IDSDSSKILGNCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRDGALRLENSKIVYLYD 720
D + P LH F W DV SVK L EIS TL++KRFR+GAL+LENSK V+L+D
Sbjct: 661 SDVAEN----GWPALHGSFKWCDVTRSVKQLSEISTTLRQKRFRNGALQLENSKPVFLFD 720
Query: 721 EYGIPYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTYPNSALLRRQPEPILRKLREFE 780
E+G+PYD +K SNFLVEEFMLLAN T AEVIS+ YP S+LLRR PEP RKL+EFE
Sbjct: 721 EHGVPYDFVTCSRKGSNFLVEEFMLLANMTAAEVISQAYPASSLLRRHPEPNTRKLKEFE 780
Query: 781 SFCSKHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYATRPMQLATYFCNGELKD 840
FCSKHG++LD SSS + Q SLE+I L DD +I+ +YA +PMQLA+YFC G LKD
Sbjct: 781 GFCSKHGMDLDISSSGQLQDSLEKITGNLKDDSVFVDILNNYAIKPMQLASYFCTGNLKD 840
Query: 841 G-ENGSHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEAEELYLKHQGIIPKVNSDEQM 900
HYALA PLYTHFTSPLRRYPDIVVHR LAAALEAEELY K + + DE
Sbjct: 841 SVAEWGHYALAVPLYTHFTSPLRRYPDIVVHRALAAALEAEELYSKQK----QTAIDEGR 900
Query: 901 RCFTGIYFDKDAADSSEGREALSSAASRHGLPCSKLLSDVALHCNNRKLASKHVADACNK 960
CFTGI+F+KDAA+S EG+EALS AA +HG+P +++LSDVA +CN RKLA++ V DAC+K
Sbjct: 901 SCFTGIHFNKDAAESIEGKEALSVAALKHGVPSTEILSDVAAYCNERKLAARKVRDACDK 960
Query: 961 LYMWALLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLEVEWLDATSTLV 1020
LY W +LK+K++ +ARV+ LG RFM+VYI KL IERRIYYD++EGL +WL+ATSTL+
Sbjct: 961 LYTWFVLKQKEIFPCEARVMNLGSRFMTVYISKLGIERRIYYDQIEGLCADWLEATSTLI 1020
Query: 1021 LSFFGTRRPHRNRGSSIKWKALEDVALVISPCDLNVKQRTLGGMSLSESGGAGTGGASVE 1080
+ + + RG +K +++ ++SPC++ V + + + +ES A
Sbjct: 1021 VD-----KLYSKRGGRGFFKPMKEAVYLVSPCEVCVAKCSALSVHDTESPEA-------- 1054
Query: 1081 QEPNLKSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYTSSY 1129
VS + PAVFPLT++L STIPV LHAVGGDDGP+DIG RLY SSY
Sbjct: 1081 --------VSIDEVAPAVFPLTIQLFSTIPVVLHAVGGDDGPLDIGARLYMSSY 1054
BLAST of Sed0025890 vs. ExPASy Swiss-Prot
Match:
Q0WPN0 (Inactive exonuclease DIS3L2 OS=Arabidopsis thaliana OX=3702 GN=SOV PE=2 SV=1)
HSP 1 Score: 959.1 bits (2478), Expect = 4.3e-278
Identity = 552/1134 (48.68%), Postives = 725/1134 (63.93%), Query Frame = 0
Query: 1 MRGAVEQSTPDRNDDGEKEKKKK-RRYSRRSKHSASIATPVSCSSVNEISGEASECMENG 60
M+ A + + +R ++G K+K+ + ++ +RRSK S + P+ + V E +G
Sbjct: 1 MKSASSEQSVERIENGHKKKRNRPQKQNRRSKQS---SVPIEDAHVEE--------SLDG 60
Query: 61 RIDANLTAPSNSSSPTQQACQSIHPSEPGLTGRNKISFGSLPPLHISKNAELSELQYSIN 120
R + A ++SS QQ + E + ++F S+PP+ AE + S +
Sbjct: 61 RDSSRSKAKDSTSSSKQQRPNT---DELEAMRASNVAFNSMPPM----RAESGYPRRSAS 120
Query: 121 QDLYSLNTGGRIV-KSCPEQIACGKMLQLSMSEHSPPADA---NATVNNTQRKYFTSHWA 180
L S +++ KSCP+ AC E SP + ++QRK F+SHW+
Sbjct: 121 PLLSSPEVSKQLLSKSCPDPRAC---------EQSPGMNGELFQQIEGSSQRKIFSSHWS 180
Query: 181 MDDVNERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILINGIASQNRALEGDIVAIMV 240
+D V E L+KG FKALFRVNAHNR EAYCKIDG+P DILING Q+RA+EGD V I +
Sbjct: 181 LDAVTEALEKGEAFKALFRVNAHNRNEAYCKIDGVPTDILINGNVCQSRAVEGDTVVIKL 240
Query: 241 DPFTSWTMMKGTCEAHNNKHSMEDANLPAEETEKNSHNCKDKNKVNSDVKSDSYRSSSSS 300
DP + W MKG K E N P E+ +K + + V S SS
Sbjct: 241 DPLSLWPKMKGFVTESAAK--PEGTNSPPEKDDKKARQKNGIDVVEGFEDGFSKNKSSVI 300
Query: 301 DKRCCSEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHYSSIQDDVSMAIGRICTLIN 360
K + + D L + +C+ Q A+ ++C +++
Sbjct: 301 GKGAKNGVTPSSPPSLDSCLGS--FCE-----------------QKGNCSAVDKLCGILS 360
Query: 361 LYPSKRPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYTKEMKTKSCLSSSHDSGYVQ 420
+P KRPTG+VVA++EKS R++IVG L+VK ++ + E K K+ LS D YVQ
Sbjct: 361 SFPHKRPTGQVVAVVEKSLVRDSIVGLLDVKGWIHYKESDPKRCKSPLSLS---DDEYVQ 420
Query: 421 LMPNDARFPIMIVLARDLPDCIKKRLENGDVTVESELVAARIDEWVEESSAPRAQVLHVL 480
LMP D RFP +IV LP I+ RLEN D +E+ELVAA+I +W E S P AQ+ H+
Sbjct: 421 LMPADPRFPKLIVPFHVLPGSIRARLENLDPNLEAELVAAQIVDWGEGSPFPVAQITHLF 480
Query: 481 GQGSKIESHIDAILFENAIRKCEFSQDSLSCLPRTPWKIPHEELQCRRDLRNLCIFTIDP 540
G+GS++E I+AIL++N++ +FS SL+ LPR PW++P EE+Q R+DLR+LC+ TIDP
Sbjct: 481 GRGSELEPQINAILYQNSVCDSDFSPGSLTSLPRVPWEVPEEEVQRRKDLRDLCVLTIDP 540
Query: 541 SSVLDLDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQHKIPML 600
S+ DLDDALSVQ L G FRVG+HIADVS+FVLP+TALD EA+ RSTSVYL+Q KI ML
Sbjct: 541 STATDLDDALSVQSLPGGFFRVGVHIADVSYFVLPETALDTEARFRSTSVYLMQRKISML 600
Query: 601 PPLLSENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSCCKLSYEHAQDIIDGL 660
PPLLSEN+ SL+PG DRLAFS+ DL+ G V D WI RT+I SCCKLSY+HAQDIIDG
Sbjct: 601 PPLLSENVGSLSPGADRLAFSILWDLNREGDVIDRWIGRTIIRSCCKLSYDHAQDIIDGK 660
Query: 661 IDSDSSKILGNCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRDGALRLENSKIVYLYD 720
D + P LH F W DV SVK L EIS TL++KRFR+GAL+LENSK V+L+D
Sbjct: 661 SDVAEN----GWPALHGSFKWCDVTRSVKQLSEISTTLRQKRFRNGALQLENSKPVFLFD 720
Query: 721 EYGIPYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTYPNSALLRRQPEPILRKLREFE 780
E+G+PYD +K SNFLVEEFMLLAN T AEVIS+ Y S+LLRR PEP RKL+EFE
Sbjct: 721 EHGVPYDFVTCSRKGSNFLVEEFMLLANMTAAEVISQAYRASSLLRRHPEPNTRKLKEFE 780
Query: 781 SFCSKHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYATRPMQLATYFCNGELKD 840
FCSKHG++LD SSS + Q SLE+I L DD +I+ +YA +PMQLA+YFC G LKD
Sbjct: 781 GFCSKHGMDLDISSSGQLQDSLEKITGNLKDDSVFVDILNNYAIKPMQLASYFCTGNLKD 840
Query: 841 G-ENGSHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEAEELYLKHQGIIPKVNSDEQM 900
HYALA PLYTHFTSPLRRYPDIVVHR LAAALEAEELY K + + DE
Sbjct: 841 SVAEWGHYALAVPLYTHFTSPLRRYPDIVVHRALAAALEAEELYSKQK----QTAIDEGR 900
Query: 901 RCFTGIYFDKDAADSSEGREALSSAASRHGLPCSKLLSDVALHCNNRKLASKHVADACNK 960
CFTGI+F+KDAA+S EG+EALS AA +HG+P +++LSDVA +CN RKLA++ V DAC+K
Sbjct: 901 SCFTGIHFNKDAAESIEGKEALSVAALKHGVPSTEILSDVAAYCNERKLAARKVRDACDK 960
Query: 961 LYMWALLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLEVEWLDATSTLV 1020
LY W +LK+K++ +ARV+ LG RFM+VYI KL IERRIYYD++EGL +WL+ATSTL+
Sbjct: 961 LYTWFVLKQKEIFPCEARVMNLGSRFMTVYISKLGIERRIYYDQIEGLCADWLEATSTLI 1020
Query: 1021 LSFFGTRRPHRNRGSSIKWKALEDVALVISPCDLNVKQRTLGGMSLSESGGAGTGGASVE 1080
+ + + RG +K +++ ++SPC++ V + + + +ES A
Sbjct: 1021 VD-----KLYSKRGGRGFFKPMKEAVYLVSPCEVCVAKCSALSVHDTESPEA-------- 1054
Query: 1081 QEPNLKSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYTSSY 1129
VS + PAVFPLT++L STIPV LHAVGGDDGP+DIG RLY SSY
Sbjct: 1081 --------VSIDEVAPAVFPLTIQLFSTIPVVLHAVGGDDGPLDIGARLYMSSY 1054
BLAST of Sed0025890 vs. ExPASy Swiss-Prot
Match:
Q8IYB7 (DIS3-like exonuclease 2 OS=Homo sapiens OX=9606 GN=DIS3L2 PE=1 SV=4)
HSP 1 Score: 419.5 bits (1077), Expect = 1.2e-115
Identity = 286/841 (34.01%), Postives = 420/841 (49.94%), Query Frame = 0
Query: 159 NATVNNTQRKYFTSHWAMDDVNERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILING 218
N + ++ F ++ + +DV+E L++G + + + R+N EA+ DI I+G
Sbjct: 39 NRSTRGKKKSIFETYMSKEDVSEGLKRGTLIQGVLRINPKKFHEAFIPSPDGDRDIFIDG 98
Query: 219 IASQNRALEGDIVAIMVDPFTSWTMMKGTCEAHNNKHSMEDANLPAEETEKN--SHNCKD 278
+ ++NRAL GD+V + + P W ++K + + E +++P E + + K
Sbjct: 99 VVARNRALNGDLVVVKLLPEEHWKVVKPESNDKETEAAYE-SDIPEELCGHHLPQQSLKS 158
Query: 279 KNKVNSDVKSDSYRSSSSSDKRCCSEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHY 338
N + + S S D ++ ++DG + C + G
Sbjct: 159 YNDSPDVIVEAQFDGSDSEDGHGITQNVLVDG------VKKLSVCVSEKGREDGD----- 218
Query: 339 SSIQDDVSMAIGRICTLINLYPSKRPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYT 398
+ + D + I + ++ S + + +VV I+EK R T
Sbjct: 219 APVTKDETTCISQDTRALS-EKSLQRSAKVVYILEKKHSRAA-----------------T 278
Query: 399 KEMKTKSCLSSSHDSGYVQLMPNDARFPIMIVLARDLPDCIKKRLENGDVTVESELVAAR 458
+K + +S Y P+D R P + V +D P R ++ + L R
Sbjct: 279 GFLKLLADKNSELFRKYALFSPSDHRVPRIYVPLKDCPQDFVARPKD----YANTLFICR 338
Query: 459 IDEWVEESSAPRAQVLHVLGQGSKIESHIDAILFENAIRKCEFSQDSLSCLPR-TPWKIP 518
I +W E+ + Q+ LGQ +IE + IL E + +FS + L CLP+ PW IP
Sbjct: 339 IVDWKEDCNFALGQLAKSLGQAGEIEPETEGILTEYGVDFSDFSSEVLECLPQGLPWTIP 398
Query: 519 HEELQCRRDLRNLCIFTIDPSSVLDLDDALSVQKLANGIFRVGIHIADVSHFVLPDTALD 578
EE RRDLR CIFTIDPS+ DLDDALS + LA+G F+VG+HIADVS+FV + LD
Sbjct: 399 PEEFSKRRDLRKDCIFTIDPSTARDLDDALSCKPLADGNFKVGVHIADVSYFVPEGSDLD 458
Query: 579 KEAQIRSTSVYLLQHKIPMLPPLLSENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRT 638
K A R+TSVYL+Q +PMLP LL E + SLNP D+L FS+ L G + D W RT
Sbjct: 459 KVAAERATSVYLVQKVVPMLPRLLCEELCSLNPMSDKLTFSVIWTLTPEGKILDEWFGRT 518
Query: 639 VICSCCKLSYEHAQDIIDGLIDSDSSKILGNCPQLHSQFSWLDVISSVKILHEISKTLKE 698
+I SC KLSYEHAQ +I+ + +K L P + + S +V +V LH I+K L++
Sbjct: 519 IIRSCTKLSYEHAQSMIESPTEKIPAKEL---PPISPEHSSEEVHQAVLNLHGIAKQLRQ 578
Query: 699 KRFRDGALRLENSKIVYLYD-EYGIPYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTY 758
+RF DGALRL+ K+ + D E G+P YE ++SN LVEEFMLLAN VA I R +
Sbjct: 579 QRFVDGALRLDQLKLAFTLDHETGLPQGCHIYEYRESNKLVEEFMLLANMAVAHKIHRAF 638
Query: 759 PNSALLRRQPEPILRKLREFESFCSKHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIV 818
P ALLRR P P R L + FC + GL +D SS+ +SL Q + ++
Sbjct: 639 PEQALLRRHPPPQTRMLSDLVEFCDQMGLPVDFSSAGALNKSLTQTFGDDKYSLARKEVL 698
Query: 819 ISYATRPMQLATYFCNGELKDGENGSHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEA 878
+ +RPMQ+A YFC+G L+D HYAL PLYTHFTSP+RR+ D++VHR LAAAL
Sbjct: 699 TNMCSRPMQMALYFCSGLLQDPAQFRHYALNVPLYTHFTSPIRRFADVLVHRLLAAALGY 758
Query: 879 EELYLKHQGIIPKVNSDEQMRCFTGIYFDKDAADSSEGREALSSAASRHGLPCSKLLSDV 938
RE L A L
Sbjct: 759 --------------------------------------RERLDMAPD--------TLQKQ 796
Query: 939 ALHCNNRKLASKHVADACNKLYMWALLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERRI 996
A HCN+R++ASK V + L+ L+KE L S+A V+G+ + V + + +++RI
Sbjct: 819 ADHCNDRRMASKRVQELSTSLFFAVLVKESGPLESEAMVMGILKQAFDVLVLRYGVQKRI 796
BLAST of Sed0025890 vs. ExPASy Swiss-Prot
Match:
Q8CI75 (DIS3-like exonuclease 2 OS=Mus musculus OX=10090 GN=Dis3l2 PE=1 SV=1)
HSP 1 Score: 418.3 bits (1074), Expect = 2.7e-115
Identity = 285/842 (33.85%), Postives = 421/842 (50.00%), Query Frame = 0
Query: 159 NATVNNTQRKYFTSHWAMDDVNERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILING 218
N ++ ++ F ++ + +DV+E L++G + + + R+N EA+ DI I+G
Sbjct: 39 NKSMRGKKKSIFETYMSKEDVSEGLKRGTLIQGVLRINPKKFHEAFIPSPDGDRDIFIDG 98
Query: 219 IASQNRALEGDIVAIMVDPFTSWTMMKGTCEAHNNKHSME---DANLPAEETEKNSHNCK 278
+ ++NRAL GD+V + + P W +K +N +E +A++P E +
Sbjct: 99 VVARNRALNGDLVVVKLLPEDQWKAVK----PESNDKEIEATYEADIPEEGCGHHPLQQS 158
Query: 279 DKNKVNSDVKSDSYRSSSSSDKRCCSEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAH 338
K DV ++ S S+ R + ++DG LS S+ +
Sbjct: 159 RKGWSGPDVIIEAQFDDSDSEDRHGNTSGLVDGV---KKLSISTPDRGKEDSSTPVMKDE 218
Query: 339 YSSIQDDVSMAIGRICTLINLYPSKRPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIY 398
+ I D + S + + +VV I+EK R
Sbjct: 219 NTPIPQDTRGLSEK---------SLQKSAKVVYILEKKHSRAA----------------- 278
Query: 399 TKEMKTKSCLSSSHDSGYVQLMPNDARFPIMIVLARDLPDCIKKRLENGDVTVESELVAA 458
T +K + +S Y P+D R P + V +D P R ++ + L
Sbjct: 279 TGILKLLADKNSDLFKKYALFSPSDHRVPRIYVPLKDCPQDFMTRPKD----FANTLFIC 338
Query: 459 RIDEWVEESSAPRAQVLHVLGQGSKIESHIDAILFENAIRKCEFSQDSLSCLPRT-PWKI 518
RI +W E+ + Q+ LGQ +IE + IL E + +FS + L CLP++ PW I
Sbjct: 339 RIIDWKEDCNFALGQLAKSLGQAGEIEPETEGILTEYGVDFSDFSSEVLECLPQSLPWTI 398
Query: 519 PHEELQCRRDLRNLCIFTIDPSSVLDLDDALSVQKLANGIFRVGIHIADVSHFVLPDTAL 578
P +E+ RRDLR CIFTIDPS+ DLDDAL+ ++L +G F VG+HIADVS+FV ++L
Sbjct: 399 PPDEVGKRRDLRKDCIFTIDPSTARDLDDALACRRLTDGTFEVGVHIADVSYFVPEGSSL 458
Query: 579 DKEAQIRSTSVYLLQHKIPMLPPLLSENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDR 638
DK A R+TSVYL+Q +PMLP LL E + SLNP D+L FS+ L G + + W R
Sbjct: 459 DKVAAERATSVYLVQKVVPMLPRLLCEELCSLNPMTDKLTFSVIWKLTPEGKILEEWFGR 518
Query: 639 TVICSCCKLSYEHAQDIIDGLIDSDSSKILGNCPQLHSQFSWLDVISSVKILHEISKTLK 698
T+I SC KLSY+HAQ +I+ + + L P + + S +V +V LH I+K L+
Sbjct: 519 TIIRSCTKLSYDHAQSMIENPTEKIPEEEL---PPISPEHSVEEVHQAVLNLHSIAKQLR 578
Query: 699 EKRFRDGALRLENSKIVYLYD-EYGIPYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRT 758
+RF DGALRL+ K+ + D E G+P YE +DSN LVEEFMLLAN VA I RT
Sbjct: 579 RQRFVDGALRLDQLKLAFTLDHETGLPQGCHIYEYRDSNKLVEEFMLLANMAVAHKIFRT 638
Query: 759 YPNSALLRRQPEPILRKLREFESFCSKHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNI 818
+P ALLRR P P + L + FC + GL +D SS+ +SL + + +
Sbjct: 639 FPEQALLRRHPPPQTKMLSDLVEFCDQMGLPMDVSSAGALNKSLTKTFGDDKYSLARKEV 698
Query: 819 VISYATRPMQLATYFCNGELKDGENGSHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALE 878
+ + +RPMQ+A YFC+G L+D E HYAL PLYTHFTSP+RR+ D++VHR LAAAL
Sbjct: 699 LTNMYSRPMQMALYFCSGMLQDQEQFRHYALNVPLYTHFTSPIRRFADVIVHRLLAAALG 758
Query: 879 AEELYLKHQGIIPKVNSDEQMRCFTGIYFDKDAADSSEGREALSSAASRHGLPCSKLLSD 938
E P V D L
Sbjct: 759 YSEQ--------PDVEPD--------------------------------------TLQK 794
Query: 939 VALHCNNRKLASKHVADACNKLYMWALLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERR 996
A HCN+R++ASK V + L+ L+KE L S+A V+G+ + V + + +++R
Sbjct: 819 QADHCNDRRMASKRVQELSIGLFFAVLVKESGPLESEAMVMGVLNQAFDVLVLRFGVQKR 794
BLAST of Sed0025890 vs. ExPASy Swiss-Prot
Match:
Q0V9R3 (DIS3-like exonuclease 2 OS=Xenopus tropicalis OX=8364 GN=dis3l2 PE=2 SV=2)
HSP 1 Score: 401.4 bits (1030), Expect = 3.5e-110
Identity = 275/836 (32.89%), Postives = 401/836 (47.97%), Query Frame = 0
Query: 166 QRKYFTSHWAMDDVNERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILINGIASQNRA 225
++ F ++ ++V+ L++G + + R+N EAY D+ I+G+ +NRA
Sbjct: 33 KKSVFEAYMTKEEVSAGLKRGELIQGPLRINPKKFHEAYLPSPDGVRDLFIDGVVPRNRA 92
Query: 226 LEGDIVAIMVDPFTSWTMMKGTCEAHNNKHSMEDANLPAEETEKNSH----NCKDKNKVN 285
L GD+V + + P W ++K ED + P T H + + N
Sbjct: 93 LNGDVVVVKLLPQEQWKVLKNDV--------CEDDDTPGHSTGNKQHALSPHLMKSSAKN 152
Query: 286 SDVKSDSYRSSSSSDKRCCSEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHYSSIQD 345
D+ ++ SS+ D E L G + + + + A Q + D
Sbjct: 153 PDLIIEAKVDSSAED----GHESALIGCLQKE-IKDQDKLGAIEEKT--SKQGDPKTFSD 212
Query: 346 DVSMAIGRICTLINLYPSKRPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYTKEMKT 405
D ++ ++ S+ TG + + +KS ++
Sbjct: 213 DCFQKTAKVVYILEKKHSRAATGFIKPLSDKS-----------------------SDLAR 272
Query: 406 KSCLSSSHDSGYVQLMPNDARFPIMIVLARDLPDCIKKRLENGDVTVESELVAARIDEWV 465
K L S P D R P + V D P E T + L I W
Sbjct: 273 KRALFS----------PVDHRLPRIYVPLGDCPHDFAIHPE----TYANTLFICSITAWR 332
Query: 466 EESSAPRAQVLHVLGQGSKIESHIDAILFENAIRKCEFSQDSLSCLPR-TPWKIPHEELQ 525
++S+ +++ LGQ +IE + IL E + +F L CLP+ PW IP EE Q
Sbjct: 333 DDSNFAEGKLMKSLGQAGEIEPETEGILVEYGVDFSDFPDKVLQCLPQDLPWTIPQEEFQ 392
Query: 526 CRRDLRNLCIFTIDPSSVLDLDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQI 585
R+DLRN CIFTIDP++ DLDDALS + L +G F VG+HIADVS+FV +ALD A
Sbjct: 393 KRKDLRNECIFTIDPATARDLDDALSCKPLPDGNFEVGVHIADVSYFVAEGSALDIMASE 452
Query: 586 RSTSVYLLQHKIPMLPPLLSENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSC 645
R+TSVYL+Q IPMLP LL E + SLNP DRL FS+ + G + D W R+VICSC
Sbjct: 453 RATSVYLVQKVIPMLPRLLCEELCSLNPMTDRLTFSVIWKITPQGEILDEWFGRSVICSC 512
Query: 646 CKLSYEHAQDIIDGLIDSDSSKILGNCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRD 705
KLSY+HAQ++I+ D P + Q + ++ +V LH I++ L+++RF D
Sbjct: 513 VKLSYDHAQNMIN---HPDKKIEQHELPPVSPQHTINEIHQAVLNLHLIAQNLRKQRFDD 572
Query: 706 GALRLENSKIVYLYD-EYGIPYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTYPNSAL 765
GALRL+ K+ + D E G+P Y+ +DSN LVEEFMLLAN VA I R +P AL
Sbjct: 573 GALRLDQLKLTFTLDKESGLPQGCYIYQYRDSNKLVEEFMLLANMAVAHHIYRRFPEEAL 632
Query: 766 LRRQPEPILRKLREFESFCSKHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYAT 825
LRR P P + L + FC + GL+LD SSS +SL + ++ + +
Sbjct: 633 LRRHPPPQTKMLNDLIEFCDQMGLQLDFSSSGTLHKSLNDQFETDEYSAARKEVLTNMCS 692
Query: 826 RPMQLATYFCNGELKDGENGSHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEAEELYL 885
RPMQ+A YFC G LKD HYAL PLYTHFTSP+RR+ D++VHR LAA+L
Sbjct: 693 RPMQMAVYFCTGALKDETLFHHYALNVPLYTHFTSPIRRFADVIVHRLLAASL------- 752
Query: 886 KHQGIIPKVNSDEQMRCFTGIYFDKDAADSSEGREALSSAASRHGLPCSKLLSDVALHCN 945
G P + ++ ++ A HCN
Sbjct: 753 ---GCGPPLKMPKE------------------------------------VIQKQADHCN 767
Query: 946 NRKLASKHVADACNKLYMWALLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERRIY 996
+RK ASK V + +L+ +KE L S+A V+G+ V + + +++RIY
Sbjct: 813 DRKTASKRVQELSAELFFSVFVKECGPLESEAMVMGVLNEAFDVIVLRFGVQKRIY 767
BLAST of Sed0025890 vs. ExPASy TrEMBL
Match:
A0A6J1JX50 (DIS3-like exonuclease 2 OS=Cucurbita maxima OX=3661 GN=LOC111489125 PE=3 SV=1)
HSP 1 Score: 1848.9 bits (4788), Expect = 0.0e+00
Identity = 930/1131 (82.23%), Postives = 1011/1131 (89.39%), Query Frame = 0
Query: 1 MRGAVEQSTPDRNDDGEKEKKKKRRYSRRSKHSASIATPVSCSSVNEISGEASECMENGR 60
MRGAVEQSTP+R DDG+KEKKKKRR +RRSK +ASI+T VSCSSVN +SGEASECMENG+
Sbjct: 1 MRGAVEQSTPERYDDGDKEKKKKRRSNRRSKQNASISTSVSCSSVNGMSGEASECMENGK 60
Query: 61 IDANLTAPSNSSSPTQQACQSIHPSEPGLTGRNKISFGSLPPLHISKNAELSELQYSINQ 120
IDANLTAPSN SS TQQA +S HP E G+T RNKI+F SLPPLHIS+ AELSE Q IN+
Sbjct: 61 IDANLTAPSNHSSLTQQAHESNHPIEHGVTRRNKIAFSSLPPLHISEQAELSESQNLINE 120
Query: 121 DLYSLNTGGRIVKSCPEQIACGKMLQLSMSEHSPPADANATVNNTQRKYFTSHWAMDDVN 180
+L+ L+ GG+ +KSCPEQI CG+M +S ++HS PAD T NN+QRKYF SHW+++DV+
Sbjct: 121 NLHPLDAGGKTIKSCPEQIVCGRMPGISTNQHSSPAD--VTENNSQRKYFASHWSVEDVD 180
Query: 181 ERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILINGIASQNRALEGDIVAIMVDPFTS 240
E LQKG IF+A FRVNAHNRLEAYCKIDGLPVD+LINGIASQNRA+EGDIVAIMVDP TS
Sbjct: 181 EGLQKGDIFRAFFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIMVDPLTS 240
Query: 241 WTMMKGTCEAHNNKHSMEDANLPAEETEKNSHNCKDKNKVNSDVKSDSYRSSSSSDKRCC 300
WT MKGT E HN+ HSMEDANLPAE TE + NCK KNKV++ VKSDS+RSSSS DKRCC
Sbjct: 241 WTRMKGTSETHNSTHSMEDANLPAEATENDGRNCKGKNKVDASVKSDSFRSSSSPDKRCC 300
Query: 301 SEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHYSSIQDDVSMAIGRICTLINLYPSK 360
SE+K+LDGTACDDLL E D Y V +AHYSS QDDVS AI RIC +I+ YP K
Sbjct: 301 SEDKILDGTACDDLLLKNEQRDVYQSLVVDLPEAHYSSNQDDVSKAIQRICAVISSYPGK 360
Query: 361 RPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYTKEMKTKSCLSSSHDSGYVQLMPND 420
RPTGRVVAI+EKSR RE+IVGHLNVKKFLSF EIY KEM TKSCLS SH+ GYVQLMPND
Sbjct: 361 RPTGRVVAILEKSRQRESIVGHLNVKKFLSFQEIYMKEMNTKSCLSPSHNCGYVQLMPND 420
Query: 421 ARFPIMIVLARDLPDCIKKRLENGDVTVESELVAARIDEWVEESSAPRAQVLHVLGQGSK 480
ARFPIM+VLA DLPD IKKRL+NGDVTVE+ELVA +I EWV+ESSAP+A VLHVLG+GS+
Sbjct: 421 ARFPIMVVLAGDLPDSIKKRLDNGDVTVENELVAVKIHEWVKESSAPQAHVLHVLGRGSE 480
Query: 481 IESHIDAILFENAIRKCEFSQDSLSCLPRTPWKIPHEELQCRRDLRNLCIFTIDPSSVLD 540
+ SHIDAILFENAI CEFS DSL+CLP TPWKIPHEELQCRRDLRNLCIFTIDPSS D
Sbjct: 481 VASHIDAILFENAIHSCEFSNDSLACLPHTPWKIPHEELQCRRDLRNLCIFTIDPSSASD 540
Query: 541 LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQHKIPMLPPLLS 600
LDDALSVQKLANGIFRVG+HIADVSHFVLPDTALDKEAQIRSTSVYLLQ KIPMLPPLLS
Sbjct: 541 LDDALSVQKLANGIFRVGVHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS 600
Query: 601 ENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSCCKLSYEHAQDIIDGLIDSDS 660
EN+ SL+PGVDRLAFSLFLD+D+CG VKD WI RTVICSCCKLSYEHAQDIIDGLIDSDS
Sbjct: 601 ENVGSLSPGVDRLAFSLFLDIDNCGDVKDRWIGRTVICSCCKLSYEHAQDIIDGLIDSDS 660
Query: 661 SKILG-NCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRDGALRLENSKIVYLYDEYGI 720
SK LG N PQLH QF WLDVISSVKILHEISKTLK+KRFRDGALRLENSKIVYLYDEYGI
Sbjct: 661 SKNLGNNYPQLHGQFEWLDVISSVKILHEISKTLKKKRFRDGALRLENSKIVYLYDEYGI 720
Query: 721 PYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTYPNSALLRRQPEPILRKLREFESFCS 780
PYDS FYEQKDSNFLVEEFMLLAN TVAEVISRT+P+SALLRR PEPILRKLREFESFCS
Sbjct: 721 PYDSAFYEQKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPILRKLREFESFCS 780
Query: 781 KHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYATRPMQLATYFCNGELKDGENG 840
KHG ELDTSSSV+FQQSLEQIRIKLHDDP LF+I+ SYATRPMQLATYFC+GELKDGE G
Sbjct: 781 KHGFELDTSSSVQFQQSLEQIRIKLHDDPLLFDILTSYATRPMQLATYFCSGELKDGEKG 840
Query: 841 SHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEAEELYLKHQGIIPKVNSDEQMRCFTG 900
SHYALA PLYTHFTSPLRRYPDI+VHRTLAAA+EAE+LYLKH+GI KVNSDEQ+RCFTG
Sbjct: 841 SHYALAVPLYTHFTSPLRRYPDIIVHRTLAAAIEAEKLYLKHRGITQKVNSDEQIRCFTG 900
Query: 901 IYFDKDAADSSEGREALSSAASRHGLPCSKLLSDVALHCNNRKLASKHVADACNKLYMWA 960
+YFDKDAADS EG+EALSSAA RHG+PC+KLL+DVALHCNNRKLASKHVAD C+KLYMWA
Sbjct: 901 MYFDKDAADSLEGKEALSSAALRHGVPCAKLLADVALHCNNRKLASKHVADGCDKLYMWA 960
Query: 961 LLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLEVEWLDATSTLVLSFFG 1020
LLK+KKVLFSDARVLGLGPRFMS+YIQKLAIERRIYYDE EGL VEWL+ TSTLVLSFFG
Sbjct: 961 LLKKKKVLFSDARVLGLGPRFMSLYIQKLAIERRIYYDETEGLAVEWLETTSTLVLSFFG 1020
Query: 1021 TRRPHRNRGSSIKWKALEDVALVISPCDLNVKQRTLGGMSLSESGGAGTGGASVEQEPNL 1080
TRR HR+RG SIKWKALEDVALV+SPCD NVKQR LG S SE GG GTGGA VEQE NL
Sbjct: 1021 TRRSHRSRG-SIKWKALEDVALVVSPCDQNVKQRALGA-SPSELGGTGTGGAVVEQESNL 1080
Query: 1081 KSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYTSSYLK 1131
KSHVSDTGIDPAVFPLTVRLLST+PVALHAVGGDDGPIDIGVRLY SSYLK
Sbjct: 1081 KSHVSDTGIDPAVFPLTVRLLSTLPVALHAVGGDDGPIDIGVRLYMSSYLK 1127
BLAST of Sed0025890 vs. ExPASy TrEMBL
Match:
A0A6J1FJM2 (DIS3-like exonuclease 2 OS=Cucurbita moschata OX=3662 GN=LOC111444646 PE=3 SV=1)
HSP 1 Score: 1840.5 bits (4766), Expect = 0.0e+00
Identity = 926/1131 (81.87%), Postives = 1013/1131 (89.57%), Query Frame = 0
Query: 1 MRGAVEQSTPDRNDDGEKEKKKKRRYSRRSKHSASIATPVSCSSVNEISGEASECMENGR 60
MRGAVEQSTP+R DDG+KEKKKKRR +RRSK +ASI+T VSCSSVN + GEASEC ENG+
Sbjct: 1 MRGAVEQSTPERYDDGDKEKKKKRRSNRRSKQNASISTSVSCSSVNGMPGEASECRENGK 60
Query: 61 IDANLTAPSNSSSPTQQACQSIHPSEPGLTGRNKISFGSLPPLHISKNAELSELQYSINQ 120
I+ANLTAPSN SS TQQA +S HP E G+T RNKI+F SLPPLHIS+ AELSE Q IN+
Sbjct: 61 INANLTAPSNHSSLTQQAHESNHPIEHGVTRRNKIAFSSLPPLHISEQAELSESQNLINE 120
Query: 121 DLYSLNTGGRIVKSCPEQIACGKMLQLSMSEHSPPADANATVNNTQRKYFTSHWAMDDVN 180
+L+ L+ GG+ +KSCPEQI CG+M +S+++HSPPAD T NNTQRKYF SHW+++DV+
Sbjct: 121 NLHPLDAGGKTIKSCPEQIVCGRMPGISINQHSPPAD--VTENNTQRKYFASHWSVEDVD 180
Query: 181 ERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILINGIASQNRALEGDIVAIMVDPFTS 240
E LQKG IF+A FRVN+HNRLEAYCKIDGLPVD+LINGIASQNRA+EGDIVAIMVDP TS
Sbjct: 181 EGLQKGDIFRAFFRVNSHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIMVDPLTS 240
Query: 241 WTMMKGTCEAHNNKHSMEDANLPAEETEKNSHNCKDKNKVNSDVKSDSYRSSSSSDKRCC 300
WT MKGT E HN+ HSMEDANLPAE TE + NCK KNK+++ VKSDS+RSSSS DKRCC
Sbjct: 241 WTRMKGTSETHNSTHSMEDANLPAEATENDGRNCKGKNKLDASVKSDSFRSSSSPDKRCC 300
Query: 301 SEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHYSSIQDDVSMAIGRICTLINLYPSK 360
SE+K+LDGTACDDLL E D Y S V P +AHYS QDDVS AI RIC +I+ YP K
Sbjct: 301 SEDKILDGTACDDLLLKNEQRDVYQSSVVDPPEAHYSRNQDDVSKAIQRICAVISSYPGK 360
Query: 361 RPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYTKEMKTKSCLSSSHDSGYVQLMPND 420
RPTGRVVAI+EKSR RE+IVGHLNVKKFLSF EIY KEM TKSCLS SH+ GYVQLMPND
Sbjct: 361 RPTGRVVAILEKSRQRESIVGHLNVKKFLSFQEIYMKEMNTKSCLSPSHNCGYVQLMPND 420
Query: 421 ARFPIMIVLARDLPDCIKKRLENGDVTVESELVAARIDEWVEESSAPRAQVLHVLGQGSK 480
ARFPIM+VLA DLPD IKKRL+NGDVTVE+ELVA +I EWV+ESSAP+A VLHVLG+GS+
Sbjct: 421 ARFPIMMVLAGDLPDSIKKRLDNGDVTVENELVAVKIHEWVKESSAPQALVLHVLGRGSE 480
Query: 481 IESHIDAILFENAIRKCEFSQDSLSCLPRTPWKIPHEELQCRRDLRNLCIFTIDPSSVLD 540
+ SHIDAILFENAI CEFS DSL+CLP TPWKIPHEELQCRRDLRNLCIFTIDPSS D
Sbjct: 481 VASHIDAILFENAIHSCEFSNDSLACLPHTPWKIPHEELQCRRDLRNLCIFTIDPSSASD 540
Query: 541 LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQHKIPMLPPLLS 600
LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQ KIPMLPPLLS
Sbjct: 541 LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS 600
Query: 601 ENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSCCKLSYEHAQDIIDGLIDSDS 660
+N+ SL+PGVDRLAFSLFLD+D+ G VKD WI RTVICSCCKLSYEHAQDIIDGLIDSD+
Sbjct: 601 KNVGSLSPGVDRLAFSLFLDIDNSGDVKDRWIGRTVICSCCKLSYEHAQDIIDGLIDSDN 660
Query: 661 SKILG-NCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRDGALRLENSKIVYLYDEYGI 720
K LG N PQLH QF+W DVISSVK+LHEISKTLK+KRFRDGALRLENSKIVYLYDEYGI
Sbjct: 661 LKNLGNNYPQLHGQFAWPDVISSVKLLHEISKTLKKKRFRDGALRLENSKIVYLYDEYGI 720
Query: 721 PYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTYPNSALLRRQPEPILRKLREFESFCS 780
PYDSTFYEQKDSNFLVEEFMLLAN TVAEVISRT+P+SALLRR PEPILRKLREFESFCS
Sbjct: 721 PYDSTFYEQKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPILRKLREFESFCS 780
Query: 781 KHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYATRPMQLATYFCNGELKDGENG 840
KHG ELDTSSSV+FQQSLEQIRIKLHDDP LF+I+ SYATRPMQLATYFC+GELKDGE G
Sbjct: 781 KHGFELDTSSSVQFQQSLEQIRIKLHDDPLLFDILTSYATRPMQLATYFCSGELKDGEKG 840
Query: 841 SHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEAEELYLKHQGIIPKVNSDEQMRCFTG 900
SHYALA PLYTHFTSPLRRYPDIVVHRTLAAA+EAE+LYLKH+GII KVNSDEQ+RCFTG
Sbjct: 841 SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKLYLKHRGIIQKVNSDEQIRCFTG 900
Query: 901 IYFDKDAADSSEGREALSSAASRHGLPCSKLLSDVALHCNNRKLASKHVADACNKLYMWA 960
+YFDKDAADS EGREALSSAA RHG+PC+KLL+DVALHCN+RKLASKHVAD C+KLYMWA
Sbjct: 901 MYFDKDAADSLEGREALSSAALRHGVPCAKLLADVALHCNDRKLASKHVADGCDKLYMWA 960
Query: 961 LLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLEVEWLDATSTLVLSFFG 1020
+LK+KKVLFSDARVLGLGPRFMS+YIQKLAIERRIYYDE EGL VEWL+ TSTLVLSFFG
Sbjct: 961 VLKKKKVLFSDARVLGLGPRFMSLYIQKLAIERRIYYDETEGLAVEWLETTSTLVLSFFG 1020
Query: 1021 TRRPHRNRGSSIKWKALEDVALVISPCDLNVKQRTLGGMSLSESGGAGTGGASVEQEPNL 1080
TRR HR+RG SIKWKALEDVALV+SPCD NVKQR L G+S SE GG GTGGA VEQE NL
Sbjct: 1021 TRRSHRSRG-SIKWKALEDVALVVSPCDHNVKQRAL-GVSPSELGGTGTGGAVVEQESNL 1080
Query: 1081 KSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYTSSYLK 1131
KSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLY SSYLK
Sbjct: 1081 KSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYMSSYLK 1127
BLAST of Sed0025890 vs. ExPASy TrEMBL
Match:
A0A0A0KC80 (DIS3-like exonuclease 2 OS=Cucumis sativus OX=3659 GN=Csa_6G040560 PE=3 SV=1)
HSP 1 Score: 1805.4 bits (4675), Expect = 0.0e+00
Identity = 909/1132 (80.30%), Postives = 1005/1132 (88.78%), Query Frame = 0
Query: 1 MRGAVEQSTPDRNDDGEKEKKKKRRYSRRSKHSASIATPVSCSSVNEISGEASECMENGR 60
MR AVEQSTP+RN DG+KEKKKKRR +RRSKH+ S+ T S +SVN I GEASECMENGR
Sbjct: 1 MRAAVEQSTPERNGDGDKEKKKKRRSNRRSKHNPSLTTSASYTSVNGILGEASECMENGR 60
Query: 61 IDANLTAPSNSSSPTQQACQSIHPSEPGLTGRNKISFGSLPPLHISKNAELSELQYSINQ 120
IDANLT+PSN SS TQQ S E GLT +KI F SLPPLHI++ AELS +NQ
Sbjct: 61 IDANLTSPSNYSSLTQQENHSNQQIEHGLTRGDKIGFSSLPPLHINEQAELSASHNLMNQ 120
Query: 121 DLYSLNTGGRIVKSCPEQIACGKMLQLSMSEHSPPADANATVNNTQRKYFTSHWAMDDVN 180
+ +S + GGR+ KSCPEQIA G+ +S+++HSPPAD T NNTQRKYF SHW++DDVN
Sbjct: 121 NHHSSDAGGRVTKSCPEQIASGRYSGISLNQHSPPAD--VTDNNTQRKYFPSHWSVDDVN 180
Query: 181 ERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILINGIASQNRALEGDIVAIMVDPFTS 240
E LQKG IFKALFRVNAHNRLEAYCKIDGLP+D+LINGIASQNRA+EGDIVAI +DPFTS
Sbjct: 181 EGLQKGGIFKALFRVNAHNRLEAYCKIDGLPIDVLINGIASQNRAVEGDIVAIKLDPFTS 240
Query: 241 WTMMKGTCEAHNNKHSMEDANLPAEETEKNSHNCKDKNKVNSDVKSDSYRSSSSSDKRCC 300
WT MKGT EAHNN HSMEDANLPAE TEKN HNCK KNKV++DVKSDS+RS+S DKRCC
Sbjct: 241 WTKMKGTSEAHNNMHSMEDANLPAELTEKNDHNCKGKNKVDADVKSDSFRSTSLPDKRCC 300
Query: 301 SEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHYSSIQDDVSMAIGRICTLINLYPSK 360
SE+KVLDG ACD LLSNYE CD S V PSQAH+SS QDDVS AIGRIC LINLYP+K
Sbjct: 301 SEDKVLDGVACDVLLSNYEQCDINELSVVNPSQAHHSSNQDDVSKAIGRICALINLYPAK 360
Query: 361 RPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYTKEMKTKSCLSSSHDSGYVQLMPND 420
RPTGRVV I+EKSR RE +VGHLNVKKFLSF E Y KE TKSCLS S + GYVQLMPND
Sbjct: 361 RPTGRVVTILEKSRLRENVVGHLNVKKFLSFQEFYVKE-STKSCLSPSQNCGYVQLMPND 420
Query: 421 ARFPIMIVLARDLPDCIKKRLENGDVTVESELVAARIDEWVEESSAPRAQVLHVLGQGSK 480
ARFPIM+VLA DLP+CIKKRL+NGDVTVE+ELVAARI EWV+ESS+PRA VLHVLG+G++
Sbjct: 421 ARFPIMMVLAGDLPNCIKKRLDNGDVTVENELVAARIYEWVKESSSPRAHVLHVLGRGNE 480
Query: 481 IESHIDAILFENAIRKCEFSQDSLSCLPRTPWKIPHEELQCRRDLRNLCIFTIDPSSVLD 540
+ESHIDAILFENAIR CEFSQDSLSC+P+TPWKIP EELQCRRD+RNLCIFTIDPSS D
Sbjct: 481 VESHIDAILFENAIRTCEFSQDSLSCVPQTPWKIPPEELQCRRDIRNLCIFTIDPSSASD 540
Query: 541 LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQHKIPMLPPLLS 600
LDDALSVQ+LANGIFRVGIHIADVS+FVLPDTALDKEAQIRSTSVYLLQ KIPMLPPLLS
Sbjct: 541 LDDALSVQRLANGIFRVGIHIADVSYFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS 600
Query: 601 ENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSCCKLSYEHAQDIIDGLIDSDS 660
E++ SLNPGVDRLAFSLFLD++ CG VKD+WI+RTVIC CCKLSYEHAQDIIDGLIDSDS
Sbjct: 601 ESIGSLNPGVDRLAFSLFLDINSCGDVKDFWIERTVICCCCKLSYEHAQDIIDGLIDSDS 660
Query: 661 SKILG-NCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRDGALRLENSKIVYLYDEYGI 720
S++ G NCPQLH QF+W DVISSVK+LHEISKT+KEKRFR+GALRLENSK++YLYDEYGI
Sbjct: 661 SELFGNNCPQLHGQFTWHDVISSVKLLHEISKTVKEKRFRNGALRLENSKLIYLYDEYGI 720
Query: 721 PYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTYPNSALLRRQPEPILRKLREFESFCS 780
PYDS FYEQKDSNFLVEEFMLLANRTVAEVISRT+P+SALLRR PEP+LRKLREFE+FCS
Sbjct: 721 PYDSMFYEQKDSNFLVEEFMLLANRTVAEVISRTFPDSALLRRHPEPMLRKLREFETFCS 780
Query: 781 KHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYATRPMQLATYFCNGELKDGENG 840
KHG ELDTSSSV FQQSLEQIRI+L DDP LF+I+ISYATRPMQLATYFC+GELKDGE
Sbjct: 781 KHGFELDTSSSVHFQQSLEQIRIELQDDPLLFDILISYATRPMQLATYFCSGELKDGETR 840
Query: 841 SHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEAEELYLKHQGIIPKVNSDEQMRCFTG 900
SHYALA PLYTHFTSPLRRYPDIVVHRTLAAA+EAE++YLKH+G+I KVNS+E+ RCFTG
Sbjct: 841 SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKMYLKHKGVIQKVNSNEETRCFTG 900
Query: 901 IYFDKDAADSSEGREALSSAASRHGLPCSKLLSDVALHCNNRKLASKHVADACNKLYMWA 960
IYFDKDAADS EGREALSSAA +HG+PCSKLL DVALHCN+RKLASKHVAD KLYMWA
Sbjct: 901 IYFDKDAADSLEGREALSSAALKHGVPCSKLLLDVALHCNDRKLASKHVADGIEKLYMWA 960
Query: 961 LLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLEVEWLDATSTLVLSFFG 1020
LLK+KK+LFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGL VEWL+ TSTLVL FF
Sbjct: 961 LLKKKKILFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLAVEWLETTSTLVLRFFC 1020
Query: 1021 TRRPHRNRGSSIKWKALEDVALVISPCDLNVKQRTLGGMSLSESGGAGTGG-ASVEQEPN 1080
+RR HR+RG S+KWKALEDVALVISPCD NVK+RTLG +S +GGA GG A VEQ+ N
Sbjct: 1021 SRRSHRSRG-SVKWKALEDVALVISPCDQNVKERTLG---VSSNGGASKGGSAVVEQDSN 1080
Query: 1081 LKSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYTSSYLK 1131
LKSHVSDTG+DPA+FPLTVRLLSTIPVALHAVGGDDGPIDIGVRLY SSYL+
Sbjct: 1081 LKSHVSDTGVDPAIFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYMSSYLR 1125
BLAST of Sed0025890 vs. ExPASy TrEMBL
Match:
A0A1S3BDZ0 (DIS3-like exonuclease 2 OS=Cucumis melo OX=3656 GN=LOC103488626 PE=3 SV=1)
HSP 1 Score: 1797.3 bits (4654), Expect = 0.0e+00
Identity = 912/1132 (80.57%), Postives = 998/1132 (88.16%), Query Frame = 0
Query: 1 MRGAVEQSTPDRNDDGEKEKKKKRRYSRRSKHSASIATPVSCSSVNEISGEASECMENGR 60
MR A EQSTP+RN D +KEKKKKRR +RRSKH+ S+ T S +SVN I GEASECMENGR
Sbjct: 1 MRAAFEQSTPERNGDCDKEKKKKRRSNRRSKHNPSLTTSASYTSVNGILGEASECMENGR 60
Query: 61 IDANLTAPSNSSSPTQQACQSIHPSEPGLTGRNKISFGSLPPLHISKNAELSELQYSINQ 120
IDANLT+PSN SS TQQ S HP E GLTG NKI+F SLP LHI+ AELS Q INQ
Sbjct: 61 IDANLTSPSNYSSLTQQENHSNHPIEHGLTGGNKIAFSSLPSLHINDQAELSASQNLINQ 120
Query: 121 DLYSLNTGGRIVKSCPEQIACGKMLQLSMSEHSPPADANATVNNTQRKYFTSHWAMDDVN 180
+ +S + GGRI+KSCPEQIA G+ +S ++ SPPAD T NNTQRKYF SHW++DDVN
Sbjct: 121 NHHSSDAGGRIIKSCPEQIASGRNSGISSNQLSPPAD--LTENNTQRKYFPSHWSIDDVN 180
Query: 181 ERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILINGIASQNRALEGDIVAIMVDPFTS 240
E LQKG IFKALFRVNAHNRLEAYCKIDGLPVD+LINGIASQNRA+EGDIVAI VDPFT
Sbjct: 181 EGLQKGDIFKALFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIKVDPFTV 240
Query: 241 WTMMKGTCEAHNNKHSMEDANLPAEETEKNSHNCKDKNKVNSDVKSDSYRSSSSSDKRCC 300
WT MKGT EAH+N SM+DANLPAE TEKNSHNCK KNK ++D KSDS+RSSS DKRCC
Sbjct: 241 WTKMKGTSEAHDNIKSMDDANLPAEPTEKNSHNCKGKNKFDADGKSDSFRSSSLPDKRCC 300
Query: 301 SEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHYSSIQDDVSMAIGRICTLINLYPSK 360
SE+KVLDG +CDDLLSNYE CD S V PSQAH+SS Q DVS IGRIC LINLYP+K
Sbjct: 301 SEDKVLDGISCDDLLSNYEQCDINQLSVVDPSQAHHSSNQYDVSKIIGRICALINLYPAK 360
Query: 361 RPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYTKEMKTKSCLSSSHDSGYVQLMPND 420
RPTGRVV I+EKSR R+ +VGHLNVKKFLSF E Y KE TKSCLS S + GYVQLMPND
Sbjct: 361 RPTGRVVTILEKSRLRDNVVGHLNVKKFLSFQEFYVKE-NTKSCLSPSQNGGYVQLMPND 420
Query: 421 ARFPIMIVLARDLPDCIKKRLENGDVTVESELVAARIDEWVEESSAPRAQVLHVLGQGSK 480
ARFPIM+VLA DLPDCIKKRL+NGDVTVE+ELVAARI +WV+ESS+PRA VLHVLG+GS+
Sbjct: 421 ARFPIMMVLAGDLPDCIKKRLDNGDVTVENELVAARIYDWVKESSSPRAHVLHVLGRGSE 480
Query: 481 IESHIDAILFENAIRKCEFSQDSLSCLPRTPWKIPHEELQCRRDLRNLCIFTIDPSSVLD 540
+ESHIDAILFENAIR CEFS DSLSC+P TPWKIPHEEL+CRRD+RNLCIFTIDPSS D
Sbjct: 481 VESHIDAILFENAIRTCEFSHDSLSCIPHTPWKIPHEELRCRRDIRNLCIFTIDPSSASD 540
Query: 541 LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQHKIPMLPPLLS 600
LDDALSVQKLAN IFRVGIHIADVS+FVLPDTALDKEAQIRSTSVYLLQ KIPMLPPLLS
Sbjct: 541 LDDALSVQKLANDIFRVGIHIADVSYFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS 600
Query: 601 ENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSCCKLSYEHAQDIIDGLIDSDS 660
EN+ SLNPGVDRLAFSLFLD++ CG VKDYWI+RTVIC CCKLSYE+AQDIIDGLIDSDS
Sbjct: 601 ENIGSLNPGVDRLAFSLFLDINGCGDVKDYWIERTVICCCCKLSYEYAQDIIDGLIDSDS 660
Query: 661 SKILG-NCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRDGALRLENSKIVYLYDEYGI 720
+I G NCPQLH QF+W DVISSVK+LHEISKTLKEKRFRDGALRLENSK++YLYDEYGI
Sbjct: 661 PEIFGNNCPQLHGQFTWHDVISSVKLLHEISKTLKEKRFRDGALRLENSKLIYLYDEYGI 720
Query: 721 PYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTYPNSALLRRQPEPILRKLREFESFCS 780
PYDS FYEQKDSNFLVEEFMLLANRTVAEVISRT+P+SALLRR PEP+LRKLREFESFCS
Sbjct: 721 PYDSMFYEQKDSNFLVEEFMLLANRTVAEVISRTFPDSALLRRHPEPMLRKLREFESFCS 780
Query: 781 KHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYATRPMQLATYFCNGELKDGENG 840
KHG ELDTSSSV FQQSLEQIR KLHDDP LF+I+ISYATRPMQLATYFC+GELKDGE
Sbjct: 781 KHGFELDTSSSVHFQQSLEQIRTKLHDDPLLFDILISYATRPMQLATYFCSGELKDGEKR 840
Query: 841 SHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEAEELYLKHQGIIPKVNSDEQMRCFTG 900
+HYALA PLYTHFTSPLRRYPDIVVHRTLAAA+EAE++YLKHQGII KVNSD++MRCFTG
Sbjct: 841 NHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKVYLKHQGIIQKVNSDKEMRCFTG 900
Query: 901 IYFDKDAADSSEGREALSSAASRHGLPCSKLLSDVALHCNNRKLASKHVADACNKLYMWA 960
IYFDKDAADS EGREALS AA +HG+PCSKLLSDVALHCN+RKLASKH+AD C KLYMWA
Sbjct: 901 IYFDKDAADSLEGREALSFAALKHGVPCSKLLSDVALHCNDRKLASKHIADGCEKLYMWA 960
Query: 961 LLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLEVEWLDATSTLVLSFFG 1020
LLK+K++LFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGL VEWLD TSTLVLSF
Sbjct: 961 LLKKKRILFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLAVEWLDTTSTLVLSFC- 1020
Query: 1021 TRRPHRNRGSSIKWKALEDVALVISPCDLNVKQRTLGGMSLSESGGAGTGG-ASVEQEPN 1080
+RR HR+RG S+KWKALEDVALVISPCD NV +RTLG + +GGA GG A+VEQ+ N
Sbjct: 1021 SRRSHRSRG-SVKWKALEDVALVISPCDQNVNKRTLG---VCPNGGASKGGSAAVEQDSN 1080
Query: 1081 LKSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYTSSYLK 1131
LKSHVSD G+DPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLY SSYL+
Sbjct: 1081 LKSHVSDIGVDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYMSSYLR 1124
BLAST of Sed0025890 vs. ExPASy TrEMBL
Match:
A0A5D3CLM1 (DIS3-like exonuclease 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1827G00340 PE=3 SV=1)
HSP 1 Score: 1786.9 bits (4627), Expect = 0.0e+00
Identity = 905/1122 (80.66%), Postives = 990/1122 (88.24%), Query Frame = 0
Query: 1 MRGAVEQSTPDRNDDGEKEKKKKRRYSRRSKHSASIATPVSCSSVNEISGEASECMENGR 60
MR A EQSTP+RN D +KEKKKKRR +RRSKH+ S+ T S +SVN I GEASECMENGR
Sbjct: 1 MRAAFEQSTPERNGDCDKEKKKKRRSNRRSKHNPSLTTSASYTSVNGILGEASECMENGR 60
Query: 61 IDANLTAPSNSSSPTQQACQSIHPSEPGLTGRNKISFGSLPPLHISKNAELSELQYSINQ 120
IDANLT+PSN SS TQQ S HP E GLTG NKI+F SLP LHI+ AELS Q INQ
Sbjct: 61 IDANLTSPSNYSSLTQQENHSNHPIEHGLTGGNKIAFSSLPSLHINDQAELSASQNLINQ 120
Query: 121 DLYSLNTGGRIVKSCPEQIACGKMLQLSMSEHSPPADANATVNNTQRKYFTSHWAMDDVN 180
+ +S + GGRI+KSCPEQIA G+ +S ++ SPPAD T NNTQRKYF SHW++DDVN
Sbjct: 121 NHHSSDAGGRIIKSCPEQIASGRNSGISSNQLSPPAD--LTENNTQRKYFPSHWSIDDVN 180
Query: 181 ERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILINGIASQNRALEGDIVAIMVDPFTS 240
E LQKG IFKALFRVNAHNRLEAYCKIDGLPVD+LINGIASQNRA+EGDIVAI VDPFT
Sbjct: 181 EGLQKGDIFKALFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIKVDPFTV 240
Query: 241 WTMMKGTCEAHNNKHSMEDANLPAEETEKNSHNCKDKNKVNSDVKSDSYRSSSSSDKRCC 300
WT MKGT EAH+N SM+DANLPAE TEKNSHNCK KNK ++D KSDS+RSSS DKRCC
Sbjct: 241 WTKMKGTSEAHDNIKSMDDANLPAEPTEKNSHNCKGKNKFDADGKSDSFRSSSLPDKRCC 300
Query: 301 SEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHYSSIQDDVSMAIGRICTLINLYPSK 360
SE+KVLDG +CDDLLSNYE CD S V PSQAH+SS Q DVS IGRIC LINLYP+K
Sbjct: 301 SEDKVLDGISCDDLLSNYEQCDINQLSVVDPSQAHHSSNQYDVSKIIGRICALINLYPAK 360
Query: 361 RPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYTKEMKTKSCLSSSHDSGYVQLMPND 420
RPTGRVV I+EKSR R+ +VGHLNVKKFLSF E Y KE TKSCLS S + GYVQLMPND
Sbjct: 361 RPTGRVVTILEKSRLRDNVVGHLNVKKFLSFQEFYVKE-NTKSCLSPSQNGGYVQLMPND 420
Query: 421 ARFPIMIVLARDLPDCIKKRLENGDVTVESELVAARIDEWVEESSAPRAQVLHVLGQGSK 480
ARFPIM+VLA DLPDCIKKRL+NGDVTVE+ELVAARI +WV+ESS+PRA VLHVLG+GS+
Sbjct: 421 ARFPIMMVLAGDLPDCIKKRLDNGDVTVENELVAARIYDWVKESSSPRAHVLHVLGRGSE 480
Query: 481 IESHIDAILFENAIRKCEFSQDSLSCLPRTPWKIPHEELQCRRDLRNLCIFTIDPSSVLD 540
+ESHIDAILFENAIR CEFS DSLSC+P TPWKIPHEEL+CRRD+RNLCIFTIDPSS D
Sbjct: 481 VESHIDAILFENAIRTCEFSHDSLSCIPHTPWKIPHEELRCRRDIRNLCIFTIDPSSASD 540
Query: 541 LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQHKIPMLPPLLS 600
LDDALSVQKLAN IFRVGIHIADVS+FVLPDTALDKEAQIRSTSVYLLQ KIPMLPPLLS
Sbjct: 541 LDDALSVQKLANDIFRVGIHIADVSYFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS 600
Query: 601 ENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSCCKLSYEHAQDIIDGLIDSDS 660
EN+ SLNPGVDRLAFSLFLD++ CG VKDYWI+RTVIC CCKLSYE+AQDIIDGLIDSDS
Sbjct: 601 ENIGSLNPGVDRLAFSLFLDINGCGDVKDYWIERTVICCCCKLSYEYAQDIIDGLIDSDS 660
Query: 661 SKILG-NCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRDGALRLENSKIVYLYDEYGI 720
+I G NCPQLH QF+W DVISSVK+LHEISKTLKEKRFRDGALRLENSK++YLYDEYGI
Sbjct: 661 PEIFGNNCPQLHGQFTWHDVISSVKLLHEISKTLKEKRFRDGALRLENSKLIYLYDEYGI 720
Query: 721 PYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTYPNSALLRRQPEPILRKLREFESFCS 780
PYDS FYEQKDSNFLVEEFMLLANRTVAEVISRT+P+SALLRR PEP+LRKLREFESFCS
Sbjct: 721 PYDSMFYEQKDSNFLVEEFMLLANRTVAEVISRTFPDSALLRRHPEPMLRKLREFESFCS 780
Query: 781 KHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYATRPMQLATYFCNGELKDGENG 840
KHG ELDTSSSV FQQSLEQIR KLHDDP LF+I+ISYATRPMQLATYFC+GELKDGE
Sbjct: 781 KHGFELDTSSSVHFQQSLEQIRTKLHDDPLLFDILISYATRPMQLATYFCSGELKDGEKR 840
Query: 841 SHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEAEELYLKHQGIIPKVNSDEQMRCFTG 900
+HYALA PLYTHFTSPLRRYPDIVVHRTLAAA+EAE++YLKHQGII KVNSD++MRCFTG
Sbjct: 841 NHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKVYLKHQGIIQKVNSDKEMRCFTG 900
Query: 901 IYFDKDAADSSEGREALSSAASRHGLPCSKLLSDVALHCNNRKLASKHVADACNKLYMWA 960
IYFDKDAADS EGREALS AA +HG+PCSKLLSDVALHCN+RKLASKH+AD C KLYMWA
Sbjct: 901 IYFDKDAADSLEGREALSFAALKHGVPCSKLLSDVALHCNDRKLASKHIADGCEKLYMWA 960
Query: 961 LLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLEVEWLDATSTLVLSFFG 1020
LLK+K++LFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGL VEWLD TSTLVLSFF
Sbjct: 961 LLKKKRILFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLAVEWLDTTSTLVLSFFC 1020
Query: 1021 TRRPHRNRGSSIKWKALEDVALVISPCDLNVKQRTLGGMSLSESGGAGTGG-ASVEQEPN 1080
+RR HR+RG S+KWKALEDVALVISPCD NV +RTLG + +GGA GG A+VEQ+ N
Sbjct: 1021 SRRSHRSRG-SVKWKALEDVALVISPCDQNVNKRTLG---VCPNGGASKGGSAAVEQDSN 1080
Query: 1081 LKSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIG 1121
LKSHVSD G+DPAVFPLTVRLLSTIPVALHAVGGDDGPIDIG
Sbjct: 1081 LKSHVSDIGVDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIG 1115
BLAST of Sed0025890 vs. TAIR 10
Match:
AT1G77680.1 (Ribonuclease II/R family protein )
HSP 1 Score: 959.1 bits (2478), Expect = 3.1e-279
Identity = 552/1134 (48.68%), Postives = 725/1134 (63.93%), Query Frame = 0
Query: 1 MRGAVEQSTPDRNDDGEKEKKKK-RRYSRRSKHSASIATPVSCSSVNEISGEASECMENG 60
M+ A + + +R ++G K+K+ + ++ +RRSK S + P+ + V E +G
Sbjct: 1 MKSASSEQSVERIENGHKKKRNRPQKQNRRSKQS---SVPIEDAHVEE--------SLDG 60
Query: 61 RIDANLTAPSNSSSPTQQACQSIHPSEPGLTGRNKISFGSLPPLHISKNAELSELQYSIN 120
R + A ++SS QQ + E + ++F S+PP+ AE + S +
Sbjct: 61 RDSSRSKAKDSTSSSKQQRPNT---DELEAMRASNVAFNSMPPM----RAESGYPRRSAS 120
Query: 121 QDLYSLNTGGRIV-KSCPEQIACGKMLQLSMSEHSPPADA---NATVNNTQRKYFTSHWA 180
L S +++ KSCP+ AC E SP + ++QRK F+SHW+
Sbjct: 121 PLLSSPEVSKQLLSKSCPDPRAC---------EQSPGMNGELFQQIEGSSQRKIFSSHWS 180
Query: 181 MDDVNERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILINGIASQNRALEGDIVAIMV 240
+D V E L+KG FKALFRVNAHNR EAYCKIDG+P DILING Q+RA+EGD V I +
Sbjct: 181 LDAVTEALEKGEAFKALFRVNAHNRNEAYCKIDGVPTDILINGNVCQSRAVEGDTVVIKL 240
Query: 241 DPFTSWTMMKGTCEAHNNKHSMEDANLPAEETEKNSHNCKDKNKVNSDVKSDSYRSSSSS 300
DP + W MKG K E N P E+ +K + + V S SS
Sbjct: 241 DPLSLWPKMKGFVTESAAK--PEGTNSPPEKDDKKARQKNGIDVVEGFEDGFSKNKSSVI 300
Query: 301 DKRCCSEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHYSSIQDDVSMAIGRICTLIN 360
K + + D L + +C+ Q A+ ++C +++
Sbjct: 301 GKGAKNGVTPSSPPSLDSCLGS--FCE-----------------QKGNCSAVDKLCGILS 360
Query: 361 LYPSKRPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYTKEMKTKSCLSSSHDSGYVQ 420
+P KRPTG+VVA++EKS R++IVG L+VK ++ + E K K+ LS D YVQ
Sbjct: 361 SFPHKRPTGQVVAVVEKSLVRDSIVGLLDVKGWIHYKESDPKRCKSPLSLS---DDEYVQ 420
Query: 421 LMPNDARFPIMIVLARDLPDCIKKRLENGDVTVESELVAARIDEWVEESSAPRAQVLHVL 480
LMP D RFP +IV LP I+ RLEN D +E+ELVAA+I +W E S P AQ+ H+
Sbjct: 421 LMPADPRFPKLIVPFHVLPGSIRARLENLDPNLEAELVAAQIVDWGEGSPFPVAQITHLF 480
Query: 481 GQGSKIESHIDAILFENAIRKCEFSQDSLSCLPRTPWKIPHEELQCRRDLRNLCIFTIDP 540
G+GS++E I+AIL++N++ +FS SL+ LPR PW++P EE+Q R+DLR+LC+ TIDP
Sbjct: 481 GRGSELEPQINAILYQNSVCDSDFSPGSLTSLPRVPWEVPEEEVQRRKDLRDLCVLTIDP 540
Query: 541 SSVLDLDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQHKIPML 600
S+ DLDDALSVQ L G FRVG+HIADVS+FVLP+TALD EA+ RSTSVYL+Q KI ML
Sbjct: 541 STATDLDDALSVQSLPGGFFRVGVHIADVSYFVLPETALDTEARFRSTSVYLMQRKISML 600
Query: 601 PPLLSENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSCCKLSYEHAQDIIDGL 660
PPLLSEN+ SL+PG DRLAFS+ DL+ G V D WI RT+I SCCKLSY+HAQDIIDG
Sbjct: 601 PPLLSENVGSLSPGADRLAFSILWDLNREGDVIDRWIGRTIIRSCCKLSYDHAQDIIDGK 660
Query: 661 IDSDSSKILGNCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRDGALRLENSKIVYLYD 720
D + P LH F W DV SVK L EIS TL++KRFR+GAL+LENSK V+L+D
Sbjct: 661 SDVAEN----GWPALHGSFKWCDVTRSVKQLSEISTTLRQKRFRNGALQLENSKPVFLFD 720
Query: 721 EYGIPYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTYPNSALLRRQPEPILRKLREFE 780
E+G+PYD +K SNFLVEEFMLLAN T AEVIS+ Y S+LLRR PEP RKL+EFE
Sbjct: 721 EHGVPYDFVTCSRKGSNFLVEEFMLLANMTAAEVISQAYRASSLLRRHPEPNTRKLKEFE 780
Query: 781 SFCSKHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYATRPMQLATYFCNGELKD 840
FCSKHG++LD SSS + Q SLE+I L DD +I+ +YA +PMQLA+YFC G LKD
Sbjct: 781 GFCSKHGMDLDISSSGQLQDSLEKITGNLKDDSVFVDILNNYAIKPMQLASYFCTGNLKD 840
Query: 841 G-ENGSHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEAEELYLKHQGIIPKVNSDEQM 900
HYALA PLYTHFTSPLRRYPDIVVHR LAAALEAEELY K + + DE
Sbjct: 841 SVAEWGHYALAVPLYTHFTSPLRRYPDIVVHRALAAALEAEELYSKQK----QTAIDEGR 900
Query: 901 RCFTGIYFDKDAADSSEGREALSSAASRHGLPCSKLLSDVALHCNNRKLASKHVADACNK 960
CFTGI+F+KDAA+S EG+EALS AA +HG+P +++LSDVA +CN RKLA++ V DAC+K
Sbjct: 901 SCFTGIHFNKDAAESIEGKEALSVAALKHGVPSTEILSDVAAYCNERKLAARKVRDACDK 960
Query: 961 LYMWALLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLEVEWLDATSTLV 1020
LY W +LK+K++ +ARV+ LG RFM+VYI KL IERRIYYD++EGL +WL+ATSTL+
Sbjct: 961 LYTWFVLKQKEIFPCEARVMNLGSRFMTVYISKLGIERRIYYDQIEGLCADWLEATSTLI 1020
Query: 1021 LSFFGTRRPHRNRGSSIKWKALEDVALVISPCDLNVKQRTLGGMSLSESGGAGTGGASVE 1080
+ + + RG +K +++ ++SPC++ V + + + +ES A
Sbjct: 1021 VD-----KLYSKRGGRGFFKPMKEAVYLVSPCEVCVAKCSALSVHDTESPEA-------- 1054
Query: 1081 QEPNLKSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYTSSY 1129
VS + PAVFPLT++L STIPV LHAVGGDDGP+DIG RLY SSY
Sbjct: 1081 --------VSIDEVAPAVFPLTIQLFSTIPVVLHAVGGDDGPLDIGARLYMSSY 1054
BLAST of Sed0025890 vs. TAIR 10
Match:
AT2G17510.1 (ribonuclease II family protein )
HSP 1 Score: 290.4 bits (742), Expect = 6.1e-78
Identity = 209/719 (29.07%), Postives = 330/719 (45.90%), Query Frame = 0
Query: 157 DANATVNNTQRKYFTSHWAMDDVNERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILI 216
DA+ + + ++ + H M ++ L +GI + RVN N EAY + + +I+I
Sbjct: 203 DADDSRPSKRKLIYQEHKPMSEITAGLHRGIYHQGKLRVNRFNPYEAYVGSESIGEEIII 262
Query: 217 NGIASQNRALEGDIVAIMVDPFTSWTMMKGTCEAHNNKHSMEDANLPAEETEKNSHNCKD 276
G ++ NRA +GDIVA+ + P W K A + +E + H D
Sbjct: 263 YGRSNMNRAFDGDIVAVELLPRDQWQDEKALSIAEED-----------DEEDDTVHLAPD 322
Query: 277 KNKVNSDVKSDSYRSSSSSDKRCCSEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHY 336
V+ ++ + +S DK
Sbjct: 323 --NVDDAPRTSNLSHETSGDKNAA------------------------------------ 382
Query: 337 SSIQDDVSMAIGRICTLINLYPSKRPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYT 396
RP+GRVV +I + ++H Y
Sbjct: 383 ----------------------PVRPSGRVVGVIRR-----------------NWHS-YC 442
Query: 397 KEMKTKSCLSSSHDSGYVQLMPNDARFPIMIVLARDLPDCIKKRLENGDVTVESELVAAR 456
++ S + S + + + D R P + + R L + + R +
Sbjct: 443 GSLEPMSLPAGSGGTAHALFVSKDRRIPKIRINTRQLQNLLDMR------------IVVA 502
Query: 457 IDEWVEESSAPRAQVLHVLGQGSKIESHIDAILFENAIRKCEFSQDSLSCLPRTPWKIPH 516
+D W +S P + +G+ E+ + +L EN + FS L+CLP PW +
Sbjct: 503 VDSWDRQSRYPSGHYVRPIGKIGDKETETEVVLIENDVDYSPFSSQVLACLPPLPWSVSS 562
Query: 517 EELQ--CRRDLRNLCIFTIDPSSVLDLDDALSVQKLANGIFRVGIHIADVSHFVLPDTAL 576
E++ R+DLR+L +F++DP D+DDAL L NG F +G+HIADV++FV P T L
Sbjct: 563 EDVSNPVRQDLRHLLVFSVDPPGCKDIDDALHCTSLPNGNFELGVHIADVTNFVHPGTPL 622
Query: 577 DKEAQIRSTSVYLLQHKIPMLPPLLSENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDR 636
D EA R TSVYL++ +I MLP L+E++ SL V+RLAFS+ ++ + +
Sbjct: 623 DDEASKRGTSVYLVERRIDMLPKPLTEDICSLRADVERLAFSVIWEMSPDAEIISTRFTK 682
Query: 637 TVICSCCKLSYEHAQDIIDGLIDSDSSKILGNCPQLHSQFSWLDVISSVKILHEISKTLK 696
++I S LSY AQ +D +DS + + ++ ++ ++K ++
Sbjct: 683 SIIKSSAALSYIEAQARMDDSRLTDS------------------LTTDLRNMNTLAKIMR 742
Query: 697 EKRFRDGALRLENSKIVYLYD-EYGIPYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRT 756
++R GAL L ++++ + D E P + Y+ ++N +VEEFML AN +VA I +
Sbjct: 743 QRRIDRGALTLASAEVKFDIDPENHDPLNIGMYQILEANQMVEEFMLAANVSVAGQILKL 798
Query: 757 YPNSALLRRQPEPILRKLREFESFCSKHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNI 816
+P+ +LLRR P P L + GL LD SSS SL++ + +DP +
Sbjct: 803 FPSCSLLRRHPTPTREMLEPLLRTAAAIGLTLDVSSSKALADSLDR---AVGEDPYFNKL 798
Query: 817 VISYATRPMQLATYFCNGELKDGENGSHYALAAPLYTHFTSPLRRYPDIVVHRTLAAAL 873
+ ATR M A YFC+G+L E HY LAAPLYTHFTSP+RRY D+ VHR LAA+L
Sbjct: 863 IRILATRCMTQAVYFCSGDLSPPEY-HHYGLAAPLYTHFTSPIRRYADVFVHRLLAASL 798
BLAST of Sed0025890 vs. TAIR 10
Match:
AT2G17510.2 (ribonuclease II family protein )
HSP 1 Score: 268.9 bits (686), Expect = 1.9e-71
Identity = 209/753 (27.76%), Postives = 333/753 (44.22%), Query Frame = 0
Query: 157 DANATVNNTQRKYFTSHWAMDDVNERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILI 216
DA+ + + ++ + H M ++ L +GI + RVN N EAY + + +I+I
Sbjct: 248 DADDSRPSKRKLIYQEHKPMSEITAGLHRGIYHQGKLRVNRFNPYEAYVGSESIGEEIII 307
Query: 217 NGIASQNRALEGDIVAIMVDPFTSWTMMKGTCEAHNNKHSMEDANLPAEETEKNSHNCKD 276
G ++ NRA +GDIVA+ + P W K A + +E + H D
Sbjct: 308 YGRSNMNRAFDGDIVAVELLPRDQWQDEKALSIAEED-----------DEEDDTVHLAPD 367
Query: 277 KNKVNSDVKSDSYRSSSSSDKRCCSEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHY 336
V+ ++ + +S DK
Sbjct: 368 --NVDDAPRTSNLSHETSGDKNAA------------------------------------ 427
Query: 337 SSIQDDVSMAIGRICTLINLYPSKRPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYT 396
RP+GRVV +I + ++H Y
Sbjct: 428 ----------------------PVRPSGRVVGVIRR-----------------NWHS-YC 487
Query: 397 KEMKTKSCLSSSHDSGYVQLMPNDARFPIMIVLARDLPDCIKKRLENGDVTVESELVAAR 456
++ S + S + + + D R P + + R L + + R +
Sbjct: 488 GSLEPMSLPAGSGGTAHALFVSKDRRIPKIRINTRQLQNLLDMR------------IVVA 547
Query: 457 IDEWVEESSAPRAQVLHVLGQ------GSKIESHID----------------AILFENAI 516
+D W +S P + +G+ +++ HI+ +L EN +
Sbjct: 548 VDSWDRQSRYPSGHYVRPIGKIGDKETETEVRDHINLFDSILVGVRWARVGKVVLIENDV 607
Query: 517 RKCEFSQDSLSCLPRTPWKIPHEELQ--CRRDLRNLCIFTIDPSSVLDLDDALSVQKLAN 576
FS L+CLP PW + E++ R+DLR+L +F++DP D+DDAL L N
Sbjct: 608 DYSPFSSQVLACLPPLPWSVSSEDVSNPVRQDLRHLLVFSVDPPGCKDIDDALHCTSLPN 667
Query: 577 GIFRVGI------------HIADVSHFVLPDTALDKEAQIRSTSVYLLQHKIPMLPPLLS 636
G F +G+ +IADV++FV P T LD EA R TSVYL++ +I MLP L+
Sbjct: 668 GNFELGVRILESSDSHKYDYIADVTNFVHPGTPLDDEASKRGTSVYLVERRIDMLPKPLT 727
Query: 637 ENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSCCKLSYEHAQDIIDGLIDSDS 696
E++ SL V+RLAFS+ ++ + +++I S LSY AQ +D +DS
Sbjct: 728 EDICSLRADVERLAFSVIWEMSPDAEIISTRFTKSIIKSSAALSYIEAQARMDDSRLTDS 787
Query: 697 SKILGNCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRDGALRLENSKIVYLYD-EYGI 756
+ + ++ ++ ++K ++++R GAL L ++++ + D E
Sbjct: 788 ------------------LTTDLRNMNTLAKIMRQRRIDRGALTLASAEVKFDIDPENHD 847
Query: 757 PYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTYPNSALLRRQPEPILRKLREFESFCS 816
P + Y+ ++N +VEEFML AN +VA I + +P+ +LLRR P P L +
Sbjct: 848 PLNIGMYQILEANQMVEEFMLAANVSVAGQILKLFPSCSLLRRHPTPTREMLEPLLRTAA 877
Query: 817 KHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYATRPMQLATYFCNGELKDGENG 873
GL LD SSS SL++ + +DP ++ ATR M A YFC+G+L E
Sbjct: 908 AIGLTLDVSSSKALADSLDR---AVGEDPYFNKLIRILATRCMTQAVYFCSGDLSPPEY- 877
BLAST of Sed0025890 vs. TAIR 10
Match:
AT5G02250.1 (Ribonuclease II/R family protein )
HSP 1 Score: 95.9 bits (237), Expect = 2.2e-19
Identity = 94/379 (24.80%), Postives = 154/379 (40.63%), Query Frame = 0
Query: 522 RRDLRNLCIFTIDPSSVLDLDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIR 581
R DL +L ++ ID +LDDALS +L +G ++ IH+AD + +V P + +D+EA+ R
Sbjct: 399 RIDLTHLKVYAIDVDEADELDDALSATRLQDGRIKIWIHVADPARYVTPGSKVDREARRR 458
Query: 582 STSVYLLQHKIPMLPPLLSENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSCC 641
TSV+L PM P L+ +SL G + A S+ + L G + +Y +D ++I
Sbjct: 459 GTSVFLPTATYPMFPEKLAMEGMSLRQGENCNAVSVSVVLRSDGCITEYSVDNSIIRPTY 518
Query: 642 KLSYEHAQDIIDGLIDSDSS-KILGNCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRD 701
L+YE A +++ ++ ++ K+L + SQ+ L K + +
Sbjct: 519 MLTYESASELLHLNLEEEAELKLLSEAAFIRSQWRREQGAVDTTTLETRIKVVNPE---- 578
Query: 702 GALRLENSKIVYLYDEYGIPYDSTFYEQKD-SNFLVEEFMLLANRTVAEVISRTYPNSAL 761
+ ++ LY E Q D + LV E M+L VA
Sbjct: 579 -----DPEPLINLYVE----------NQADLAMRLVFEMMILCGEVVA------------ 638
Query: 762 LRRQPEPILRKLREFESFCSKHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYAT 821
+F S+H + L + Q I D S F +
Sbjct: 639 ----------------TFGSQHNIPLP-------YRGQPQSNI----DVSAFAHLPEGPV 698
Query: 822 RPMQLATYFCNGELKDGENGSHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEAEELYL 881
R + E+ H L P Y FTSP+RRY D+ H + A L + +
Sbjct: 699 RSSSIVKVMRAAEMNFRCPVRHGVLGIPGYVQFTSPIRRYMDLTAHYQIKAFLRGGDNFP 719
Query: 882 ----KHQGIIPKVNSDEQM 895
+ +GI VN ++
Sbjct: 759 FSAGELEGIAASVNMQSKV 719
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| P0DM58 | 3.9e-279 | 48.77 | DIS3-like exonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=SOV PE=1 SV=1 | [more] |
| Q0WPN0 | 4.3e-278 | 48.68 | Inactive exonuclease DIS3L2 OS=Arabidopsis thaliana OX=3702 GN=SOV PE=2 SV=1 | [more] |
| Q8IYB7 | 1.2e-115 | 34.01 | DIS3-like exonuclease 2 OS=Homo sapiens OX=9606 GN=DIS3L2 PE=1 SV=4 | [more] |
| Q8CI75 | 2.7e-115 | 33.85 | DIS3-like exonuclease 2 OS=Mus musculus OX=10090 GN=Dis3l2 PE=1 SV=1 | [more] |
| Q0V9R3 | 3.5e-110 | 32.89 | DIS3-like exonuclease 2 OS=Xenopus tropicalis OX=8364 GN=dis3l2 PE=2 SV=2 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1JX50 | 0.0e+00 | 82.23 | DIS3-like exonuclease 2 OS=Cucurbita maxima OX=3661 GN=LOC111489125 PE=3 SV=1 | [more] |
| A0A6J1FJM2 | 0.0e+00 | 81.87 | DIS3-like exonuclease 2 OS=Cucurbita moschata OX=3662 GN=LOC111444646 PE=3 SV=1 | [more] |
| A0A0A0KC80 | 0.0e+00 | 80.30 | DIS3-like exonuclease 2 OS=Cucumis sativus OX=3659 GN=Csa_6G040560 PE=3 SV=1 | [more] |
| A0A1S3BDZ0 | 0.0e+00 | 80.57 | DIS3-like exonuclease 2 OS=Cucumis melo OX=3656 GN=LOC103488626 PE=3 SV=1 | [more] |
| A0A5D3CLM1 | 0.0e+00 | 80.66 | DIS3-like exonuclease 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... | [more] |
