Sed0025890.3 (mRNA) Chayote v1

BLAST of Sed0025890.3 vs. NCBI nr
Match: XP_038886229.1 (DIS3-like exonuclease 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 1859.3 bits (4815), Expect = 0.0e+00
Identity = 940/1131 (83.11%), Postives = 1016/1131 (89.83%), Query Frame = 0

Query: 1    MRGAVEQSTPDRNDDGEKEKKKKRRYSRRSKHSASIATPVSCSSVNEISGEASECMENGR 60
            MRGAVEQSTPDRN+DG+KEKKKKRR +RRSK +AS++T  SC+SVN I+GEASE MENGR
Sbjct: 1    MRGAVEQSTPDRNEDGDKEKKKKRRSNRRSKQNASLSTSASCNSVNGITGEASESMENGR 60

Query: 61   IDANLTAPSNSSSPTQQACQSIHPSEPGLTGRNKISFGSLPPLHISKNAELSELQYSINQ 120
            IDANLT+PSN SS TQQA QS HP E GLT RNKI+F SLPPLHIS+ AELSE Q   NQ
Sbjct: 61   IDANLTSPSNYSSLTQQAYQSNHPIEHGLTRRNKIAFSSLPPLHISEEAELSESQNLKNQ 120

Query: 121  DLYSLNTGGRIVKSCPEQIACGKMLQLSMSEHSPPADANATVNNTQRKYFTSHWAMDDVN 180
            +L+SL+ GGRI+KSCPEQIA G+   +S+++HSPPAD   T NN+QRKYF SHW+MDDVN
Sbjct: 121  NLHSLDDGGRIIKSCPEQIAFGRNSGISLNQHSPPAD--VTENNSQRKYFPSHWSMDDVN 180

Query: 181  ERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILINGIASQNRALEGDIVAIMVDPFTS 240
            E LQKG IFKALFRVNAHNRLEAYCKIDGLPVD+LINGIASQNRA+EGDIVAI VDPFTS
Sbjct: 181  EGLQKGDIFKALFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIKVDPFTS 240

Query: 241  WTMMKGTCEAHNNKHSMEDANLPAEETEKNSHNCKDKNKVNSDVKSDSYRSSSSSDKRCC 300
            WT MKGT EAHNN HSMED NLP E  EK+ HNCK KNKV++DVKSDS+RSSS  DKRCC
Sbjct: 241  WTRMKGTSEAHNNMHSMEDVNLPFEVAEKDCHNCKGKNKVDADVKSDSFRSSSLPDKRCC 300

Query: 301  SEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHYSSIQDDVSMAIGRICTLINLYPSK 360
            SE+KVLDGTACDDLLSNYE CD    S V PSQAH+SS QDDVS A+GRIC +INLYP+K
Sbjct: 301  SEDKVLDGTACDDLLSNYEQCDVNQSSVVYPSQAHFSSNQDDVSKAVGRICAVINLYPAK 360

Query: 361  RPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYTKEMKTKSCLSSSHDSGYVQLMPND 420
            RPTGRVV I+EKSR RET+VGHLNVKKFLSF EIY KE      LS   + GYVQL+PND
Sbjct: 361  RPTGRVVTILEKSRLRETVVGHLNVKKFLSFQEIYVKE--NTKFLSPLQNCGYVQLIPND 420

Query: 421  ARFPIMIVLARDLPDCIKKRLENGDVTVESELVAARIDEWVEESSAPRAQVLHVLGQGSK 480
            ARFPIM+VLA DLPDCIKKRL+NGD+TVESELVAARI EWV ESSAPRAQVLHVLG+GS+
Sbjct: 421  ARFPIMMVLAEDLPDCIKKRLDNGDLTVESELVAARIHEWVIESSAPRAQVLHVLGRGSE 480

Query: 481  IESHIDAILFENAIRKCEFSQDSLSCLPRTPWKIPHEELQCRRDLRNLCIFTIDPSSVLD 540
            +ESHIDAILFENAIR CEFS DSLSC+P TPWKIP EELQCRRD+RNLCIFTIDPSS  D
Sbjct: 481  VESHIDAILFENAIRTCEFSHDSLSCIPHTPWKIPQEELQCRRDIRNLCIFTIDPSSASD 540

Query: 541  LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQHKIPMLPPLLS 600
            LDDALSVQ LANGIFRVGIH+ADVSHFVLP TALDKEAQIRS SVYLLQ KIPMLPPLLS
Sbjct: 541  LDDALSVQILANGIFRVGIHVADVSHFVLPGTALDKEAQIRSMSVYLLQRKIPMLPPLLS 600

Query: 601  ENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSCCKLSYEHAQDIIDGLIDSDS 660
            EN+ SLNPGVDRLAFSLFLD+++CG VK+ WI RTVICSCCKLSYE AQDIIDGLIDSDS
Sbjct: 601  ENIGSLNPGVDRLAFSLFLDINNCGDVKECWIGRTVICSCCKLSYEQAQDIIDGLIDSDS 660

Query: 661  SKIL-GNCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRDGALRLENSKIVYLYDEYGI 720
            SKIL  NCPQLH QF+W DVISSVK+LHEISKTLKEKRFRDGALRLENSKI++LYDE GI
Sbjct: 661  SKILRNNCPQLHGQFAWHDVISSVKLLHEISKTLKEKRFRDGALRLENSKIIFLYDECGI 720

Query: 721  PYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTYPNSALLRRQPEPILRKLREFESFCS 780
            PYDSTFYEQKDSNFLVEEFMLLAN TVAEVISRT+P+SALLRR P+PILRKLREFESFCS
Sbjct: 721  PYDSTFYEQKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPDPILRKLREFESFCS 780

Query: 781  KHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYATRPMQLATYFCNGELKDGENG 840
            +HG ELDTSSSV+FQQSLEQIRIKLHDDP LF+I+ SYATRPMQLATYFC+GELKDGE G
Sbjct: 781  RHGFELDTSSSVQFQQSLEQIRIKLHDDPLLFDILTSYATRPMQLATYFCSGELKDGEKG 840

Query: 841  SHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEAEELYLKHQGIIPKVNSDEQMRCFTG 900
            SHYALA PLYTHFTSPLRRYPDIVVHRTLAAA+EAE LYLKH+GII KVNSDEQMRCFTG
Sbjct: 841  SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEMLYLKHRGIIQKVNSDEQMRCFTG 900

Query: 901  IYFDKDAADSSEGREALSSAASRHGLPCSKLLSDVALHCNNRKLASKHVADACNKLYMWA 960
            I FDKDAADS EGREALSSAA RHG+PCSKLLSDVA+HCNNRKLASKHVAD C KLYMWA
Sbjct: 901  ISFDKDAADSLEGREALSSAALRHGVPCSKLLSDVAVHCNNRKLASKHVADGCEKLYMWA 960

Query: 961  LLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLEVEWLDATSTLVLSFFG 1020
            LLK+KK+LFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGL VEWLD TSTLVLSFFG
Sbjct: 961  LLKKKKILFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLAVEWLDTTSTLVLSFFG 1020

Query: 1021 TRRPHRNRGSSIKWKALEDVALVISPCDLNVKQRTLGGMSLSESGGAGTGGASVEQEPNL 1080
            TRR HRNRG SIKWKALEDVAL+ISPCD N+KQRTL G+S SE GGA TGG++VEQE NL
Sbjct: 1021 TRRSHRNRG-SIKWKALEDVALIISPCDQNIKQRTL-GVSPSELGGATTGGSAVEQESNL 1080

Query: 1081 KSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYTSSYLK 1131
            KSHVSDTG+DPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLY SSYL+
Sbjct: 1081 KSHVSDTGVDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYMSSYLR 1125

BLAST of Sed0025890.3 vs. NCBI nr
Match: KAG7016503.1 (DIS3-like exonuclease 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1851.6 bits (4795), Expect = 0.0e+00
Identity = 931/1131 (82.32%), Postives = 1015/1131 (89.74%), Query Frame = 0

Query: 1    MRGAVEQSTPDRNDDGEKEKKKKRRYSRRSKHSASIATPVSCSSVNEISGEASECMENGR 60
            MRGAVEQSTP+R DDG+KEKKKKRR +RRSK +ASI+T VSCSSVN +SGEASECMENG+
Sbjct: 1    MRGAVEQSTPERYDDGDKEKKKKRRSNRRSKQNASISTSVSCSSVNGMSGEASECMENGK 60

Query: 61   IDANLTAPSNSSSPTQQACQSIHPSEPGLTGRNKISFGSLPPLHISKNAELSELQYSINQ 120
            IDANLTAPSN SS TQQA +S HP E G+T RNKI+F SLPPLHIS+ AELSE Q  IN+
Sbjct: 61   IDANLTAPSNHSSLTQQAHESNHPIEHGVTRRNKIAFSSLPPLHISEQAELSESQNLINE 120

Query: 121  DLYSLNTGGRIVKSCPEQIACGKMLQLSMSEHSPPADANATVNNTQRKYFTSHWAMDDVN 180
            +L+ L+ GG+ +KSCPEQI CG+M  +S+++HSPPAD   T NNTQRKYF SHW+++DV+
Sbjct: 121  NLHPLDAGGKTIKSCPEQIVCGRMPGISINQHSPPAD--VTENNTQRKYFASHWSVEDVD 180

Query: 181  ERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILINGIASQNRALEGDIVAIMVDPFTS 240
            E LQKG IF+A FRVNAHNRLEAYCKIDGLPVD+LINGIASQNRA+EGDIVAIMVDP TS
Sbjct: 181  EGLQKGDIFRAFFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIMVDPLTS 240

Query: 241  WTMMKGTCEAHNNKHSMEDANLPAEETEKNSHNCKDKNKVNSDVKSDSYRSSSSSDKRCC 300
            W  MKGT E HN+ HSMEDANLPAE TE +  NCK KNK+++ VKSDS+RSSSS DKRCC
Sbjct: 241  WIRMKGTSETHNSTHSMEDANLPAEATENDGRNCKGKNKLDASVKSDSFRSSSSPDKRCC 300

Query: 301  SEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHYSSIQDDVSMAIGRICTLINLYPSK 360
            SE+K+LDGTACDDLL   E  D Y  S V P +AHYSS QDDVS AI RIC +I+ YP K
Sbjct: 301  SEDKILDGTACDDLLLKNEQRDVYQSSVVDPPEAHYSSNQDDVSKAIQRICAVISSYPGK 360

Query: 361  RPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYTKEMKTKSCLSSSHDSGYVQLMPND 420
            RPTGRVVAI+EKSR RE+IVGHLNVKKFLSF EIY KEM TKSCLS SH+ G+VQLMPND
Sbjct: 361  RPTGRVVAILEKSRQRESIVGHLNVKKFLSFQEIYMKEMNTKSCLSPSHNCGHVQLMPND 420

Query: 421  ARFPIMIVLARDLPDCIKKRLENGDVTVESELVAARIDEWVEESSAPRAQVLHVLGQGSK 480
            ARFPIM+VLA DLPD IKKRL+NGDVTVE+ELVA +I EWV+ESSAP+A VLHVLG+GS+
Sbjct: 421  ARFPIMMVLAGDLPDSIKKRLDNGDVTVENELVAVKIHEWVKESSAPQAHVLHVLGRGSE 480

Query: 481  IESHIDAILFENAIRKCEFSQDSLSCLPRTPWKIPHEELQCRRDLRNLCIFTIDPSSVLD 540
            + SHIDAILFENAI  CEFS DSL+CLP +PWKIPHEELQCRRDLRNLCIFTIDPSS  D
Sbjct: 481  VASHIDAILFENAIHSCEFSNDSLACLPHSPWKIPHEELQCRRDLRNLCIFTIDPSSASD 540

Query: 541  LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQHKIPMLPPLLS 600
            LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQ KIPMLPPLLS
Sbjct: 541  LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS 600

Query: 601  ENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSCCKLSYEHAQDIIDGLIDSDS 660
            EN+ SL+PGVDRLAFSLFLD+D+CG VKD WI RTVICSCCKLSYEHAQDIIDGLIDSDS
Sbjct: 601  ENVGSLSPGVDRLAFSLFLDIDNCGDVKDRWIGRTVICSCCKLSYEHAQDIIDGLIDSDS 660

Query: 661  SKILG-NCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRDGALRLENSKIVYLYDEYGI 720
            SK LG N PQLH QF+W DVISSVK+LHEISKTLK+KRFRDGALRLENSKIVYLYDEYG+
Sbjct: 661  SKNLGNNYPQLHGQFAWPDVISSVKLLHEISKTLKKKRFRDGALRLENSKIVYLYDEYGV 720

Query: 721  PYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTYPNSALLRRQPEPILRKLREFESFCS 780
            PYDSTFYEQKDSNFLVEEFMLLAN TVAEVISRT+P+SALLRR PEPILRKLREFESFCS
Sbjct: 721  PYDSTFYEQKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPILRKLREFESFCS 780

Query: 781  KHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYATRPMQLATYFCNGELKDGENG 840
            KHG ELDTSSSV+FQQSLEQIRIKLHDDP LF+I+ SYATRPMQLATYFC+GELKDGE G
Sbjct: 781  KHGFELDTSSSVQFQQSLEQIRIKLHDDPLLFDILTSYATRPMQLATYFCSGELKDGEKG 840

Query: 841  SHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEAEELYLKHQGIIPKVNSDEQMRCFTG 900
            SHYALA PLYTHFTSPLRRYPDIVVHRTLAAA+EAE+LYLKH+GII KVNSDEQ+RCFTG
Sbjct: 841  SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKLYLKHRGIIQKVNSDEQIRCFTG 900

Query: 901  IYFDKDAADSSEGREALSSAASRHGLPCSKLLSDVALHCNNRKLASKHVADACNKLYMWA 960
            +YFDKDAADS EGREALSSAA RHG+PC+KLL+DVALHCNNRKLASKHVAD C+KLYMWA
Sbjct: 901  MYFDKDAADSLEGREALSSAALRHGVPCAKLLADVALHCNNRKLASKHVADGCDKLYMWA 960

Query: 961  LLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLEVEWLDATSTLVLSFFG 1020
            LLK+KKVLFSDARVLGLGPRFMS+YIQKL IERRIYYDE EGL VEWL+ TSTLVLSFFG
Sbjct: 961  LLKKKKVLFSDARVLGLGPRFMSLYIQKLDIERRIYYDETEGLAVEWLETTSTLVLSFFG 1020

Query: 1021 TRRPHRNRGSSIKWKALEDVALVISPCDLNVKQRTLGGMSLSESGGAGTGGASVEQEPNL 1080
            TRR HR+RG SIKWKALEDVALV+SPCD NVKQR L G+S SE GG  TGGA VEQE NL
Sbjct: 1021 TRRSHRSRG-SIKWKALEDVALVVSPCDHNVKQRAL-GVSPSELGGTSTGGAVVEQESNL 1080

Query: 1081 KSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYTSSYLK 1131
            KSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLY SSYLK
Sbjct: 1081 KSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYMSSYLK 1127

BLAST of Sed0025890.3 vs. NCBI nr
Match: XP_023550262.1 (DIS3-like exonuclease 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1850.1 bits (4791), Expect = 0.0e+00
Identity = 930/1131 (82.23%), Postives = 1016/1131 (89.83%), Query Frame = 0

Query: 1    MRGAVEQSTPDRNDDGEKEKKKKRRYSRRSKHSASIATPVSCSSVNEISGEASECMENGR 60
            MRGAVEQSTP+R DDG+KEKKKKRR +RRSK +ASI+T VSCSSVN +SGEASECMENG+
Sbjct: 1    MRGAVEQSTPERYDDGDKEKKKKRRSNRRSKQNASISTSVSCSSVNGMSGEASECMENGK 60

Query: 61   IDANLTAPSNSSSPTQQACQSIHPSEPGLTGRNKISFGSLPPLHISKNAELSELQYSINQ 120
            IDANLTAPSN SS TQQA +S HP E G+T RNKI+F SLPPLHIS+ AELSE Q  IN+
Sbjct: 61   IDANLTAPSNHSSLTQQAHESNHPIEHGVTRRNKIAFSSLPPLHISEEAELSESQNLINE 120

Query: 121  DLYSLNTGGRIVKSCPEQIACGKMLQLSMSEHSPPADANATVNNTQRKYFTSHWAMDDVN 180
            +L+ L+ GG+I+KSCPEQI CG+M  +S+++HSPPAD   T NNTQRKYF SHW+++DV+
Sbjct: 121  NLHPLDAGGKIIKSCPEQIVCGRMPGISINQHSPPAD--VTENNTQRKYFASHWSVEDVD 180

Query: 181  ERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILINGIASQNRALEGDIVAIMVDPFTS 240
            E LQKG IF+ALFRVNAHNRLEAYCKIDGLPVD+LINGIASQNRA+EGDIVAIMVDP TS
Sbjct: 181  EGLQKGDIFRALFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIMVDPLTS 240

Query: 241  WTMMKGTCEAHNNKHSMEDANLPAEETEKNSHNCKDKNKVNSDVKSDSYRSSSSSDKRCC 300
            WT MKGT EAHN  HS+EDANLP E TE +  NCK KNKV++ VKSDS+RSSSS DKRCC
Sbjct: 241  WTRMKGTSEAHNCTHSVEDANLPVEATENDGRNCKGKNKVDASVKSDSFRSSSSPDKRCC 300

Query: 301  SEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHYSSIQDDVSMAIGRICTLINLYPSK 360
            SE+K+LDGTAC D+L   E  D Y  S V P +AHYSS QDDVS AI RIC +I+ YP K
Sbjct: 301  SEDKILDGTACGDVLLKNEQRDVYQSSVVDPPEAHYSSNQDDVSKAIQRICAVISSYPGK 360

Query: 361  RPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYTKEMKTKSCLSSSHDSGYVQLMPND 420
            RPTGRVVAI+EK+R RE+IVGHLNVKKFLSF EIY KEM TKSCLS SH+ GYVQLMPND
Sbjct: 361  RPTGRVVAILEKTRQRESIVGHLNVKKFLSFQEIYMKEMNTKSCLSPSHNCGYVQLMPND 420

Query: 421  ARFPIMIVLARDLPDCIKKRLENGDVTVESELVAARIDEWVEESSAPRAQVLHVLGQGSK 480
            ARFP M+VLA DLPD IKKRL+NGDVTVE+ELVA +I EWV ESSAP+A VLHVLG+GS+
Sbjct: 421  ARFPTMMVLAGDLPDSIKKRLDNGDVTVENELVAVKIHEWVNESSAPQAHVLHVLGRGSE 480

Query: 481  IESHIDAILFENAIRKCEFSQDSLSCLPRTPWKIPHEELQCRRDLRNLCIFTIDPSSVLD 540
            + SHIDAILFENAI  CEFS DSL+CLP TPWKIP EELQCRRDLRNLC+FTIDPSS  D
Sbjct: 481  VASHIDAILFENAIHSCEFSNDSLACLPHTPWKIPPEELQCRRDLRNLCVFTIDPSSASD 540

Query: 541  LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQHKIPMLPPLLS 600
            LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQ KIPMLPPLLS
Sbjct: 541  LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS 600

Query: 601  ENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSCCKLSYEHAQDIIDGLIDSDS 660
            EN+ SL+PGVDRLAFSLFLD+D+CG VKD WI RTVICSCCKLSYEHAQDIIDGLIDSDS
Sbjct: 601  ENVGSLSPGVDRLAFSLFLDIDNCGDVKDRWIGRTVICSCCKLSYEHAQDIIDGLIDSDS 660

Query: 661  SKILG-NCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRDGALRLENSKIVYLYDEYGI 720
            SK LG N PQLH QF+W DVISSVK+LHEISKTLK+KRFRDGALRLENSKIVYLYDEYGI
Sbjct: 661  SKNLGNNYPQLHGQFAWPDVISSVKLLHEISKTLKKKRFRDGALRLENSKIVYLYDEYGI 720

Query: 721  PYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTYPNSALLRRQPEPILRKLREFESFCS 780
            PYDSTFYEQKDSNFLVEEFMLLAN +VAEVISRT+P+SALLRR PEPILRKLREFESFCS
Sbjct: 721  PYDSTFYEQKDSNFLVEEFMLLANTSVAEVISRTFPDSALLRRHPEPILRKLREFESFCS 780

Query: 781  KHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYATRPMQLATYFCNGELKDGENG 840
            KHG ELDTSSSV+FQQSLEQIRIKLHDDP LF+I+ SYATRPMQLATYFC+GELKDGE G
Sbjct: 781  KHGFELDTSSSVQFQQSLEQIRIKLHDDPLLFDILTSYATRPMQLATYFCSGELKDGEKG 840

Query: 841  SHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEAEELYLKHQGIIPKVNSDEQMRCFTG 900
            SHYALA PLYTHFTSPLRRYPDIVVHRTLAAA+EAE+LYLKH+G+I KVNSDEQ+RCFTG
Sbjct: 841  SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKLYLKHRGMIQKVNSDEQIRCFTG 900

Query: 901  IYFDKDAADSSEGREALSSAASRHGLPCSKLLSDVALHCNNRKLASKHVADACNKLYMWA 960
            +YFDKDAADS EGREALSSAA RHG+PC+KLL+DVALHCNNRKLASKHVAD C+KLYMWA
Sbjct: 901  MYFDKDAADSLEGREALSSAALRHGVPCAKLLADVALHCNNRKLASKHVADGCDKLYMWA 960

Query: 961  LLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLEVEWLDATSTLVLSFFG 1020
            LLK+KKVLFSDARVLGLGPRFMS+YIQKLAIERR+YYDE EGL VEWL+ TSTLVLSFFG
Sbjct: 961  LLKKKKVLFSDARVLGLGPRFMSLYIQKLAIERRVYYDETEGLAVEWLETTSTLVLSFFG 1020

Query: 1021 TRRPHRNRGSSIKWKALEDVALVISPCDLNVKQRTLGGMSLSESGGAGTGGASVEQEPNL 1080
            TRR HR+RG SIKWKALEDVALV+SPCD NVKQRTL G+S SE GG GTGGA VEQE NL
Sbjct: 1021 TRRSHRSRG-SIKWKALEDVALVVSPCDQNVKQRTL-GVSPSELGGTGTGGAVVEQESNL 1080

Query: 1081 KSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYTSSYLK 1131
            KSHVSDTGI+PAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLY SSYLK
Sbjct: 1081 KSHVSDTGIEPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYMSSYLK 1127

BLAST of Sed0025890.3 vs. NCBI nr
Match: XP_022992945.1 (DIS3-like exonuclease 2 [Cucurbita maxima])

HSP 1 Score: 1848.9 bits (4788), Expect = 0.0e+00
Identity = 930/1131 (82.23%), Postives = 1011/1131 (89.39%), Query Frame = 0

Query: 1    MRGAVEQSTPDRNDDGEKEKKKKRRYSRRSKHSASIATPVSCSSVNEISGEASECMENGR 60
            MRGAVEQSTP+R DDG+KEKKKKRR +RRSK +ASI+T VSCSSVN +SGEASECMENG+
Sbjct: 1    MRGAVEQSTPERYDDGDKEKKKKRRSNRRSKQNASISTSVSCSSVNGMSGEASECMENGK 60

Query: 61   IDANLTAPSNSSSPTQQACQSIHPSEPGLTGRNKISFGSLPPLHISKNAELSELQYSINQ 120
            IDANLTAPSN SS TQQA +S HP E G+T RNKI+F SLPPLHIS+ AELSE Q  IN+
Sbjct: 61   IDANLTAPSNHSSLTQQAHESNHPIEHGVTRRNKIAFSSLPPLHISEQAELSESQNLINE 120

Query: 121  DLYSLNTGGRIVKSCPEQIACGKMLQLSMSEHSPPADANATVNNTQRKYFTSHWAMDDVN 180
            +L+ L+ GG+ +KSCPEQI CG+M  +S ++HS PAD   T NN+QRKYF SHW+++DV+
Sbjct: 121  NLHPLDAGGKTIKSCPEQIVCGRMPGISTNQHSSPAD--VTENNSQRKYFASHWSVEDVD 180

Query: 181  ERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILINGIASQNRALEGDIVAIMVDPFTS 240
            E LQKG IF+A FRVNAHNRLEAYCKIDGLPVD+LINGIASQNRA+EGDIVAIMVDP TS
Sbjct: 181  EGLQKGDIFRAFFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIMVDPLTS 240

Query: 241  WTMMKGTCEAHNNKHSMEDANLPAEETEKNSHNCKDKNKVNSDVKSDSYRSSSSSDKRCC 300
            WT MKGT E HN+ HSMEDANLPAE TE +  NCK KNKV++ VKSDS+RSSSS DKRCC
Sbjct: 241  WTRMKGTSETHNSTHSMEDANLPAEATENDGRNCKGKNKVDASVKSDSFRSSSSPDKRCC 300

Query: 301  SEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHYSSIQDDVSMAIGRICTLINLYPSK 360
            SE+K+LDGTACDDLL   E  D Y    V   +AHYSS QDDVS AI RIC +I+ YP K
Sbjct: 301  SEDKILDGTACDDLLLKNEQRDVYQSLVVDLPEAHYSSNQDDVSKAIQRICAVISSYPGK 360

Query: 361  RPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYTKEMKTKSCLSSSHDSGYVQLMPND 420
            RPTGRVVAI+EKSR RE+IVGHLNVKKFLSF EIY KEM TKSCLS SH+ GYVQLMPND
Sbjct: 361  RPTGRVVAILEKSRQRESIVGHLNVKKFLSFQEIYMKEMNTKSCLSPSHNCGYVQLMPND 420

Query: 421  ARFPIMIVLARDLPDCIKKRLENGDVTVESELVAARIDEWVEESSAPRAQVLHVLGQGSK 480
            ARFPIM+VLA DLPD IKKRL+NGDVTVE+ELVA +I EWV+ESSAP+A VLHVLG+GS+
Sbjct: 421  ARFPIMVVLAGDLPDSIKKRLDNGDVTVENELVAVKIHEWVKESSAPQAHVLHVLGRGSE 480

Query: 481  IESHIDAILFENAIRKCEFSQDSLSCLPRTPWKIPHEELQCRRDLRNLCIFTIDPSSVLD 540
            + SHIDAILFENAI  CEFS DSL+CLP TPWKIPHEELQCRRDLRNLCIFTIDPSS  D
Sbjct: 481  VASHIDAILFENAIHSCEFSNDSLACLPHTPWKIPHEELQCRRDLRNLCIFTIDPSSASD 540

Query: 541  LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQHKIPMLPPLLS 600
            LDDALSVQKLANGIFRVG+HIADVSHFVLPDTALDKEAQIRSTSVYLLQ KIPMLPPLLS
Sbjct: 541  LDDALSVQKLANGIFRVGVHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS 600

Query: 601  ENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSCCKLSYEHAQDIIDGLIDSDS 660
            EN+ SL+PGVDRLAFSLFLD+D+CG VKD WI RTVICSCCKLSYEHAQDIIDGLIDSDS
Sbjct: 601  ENVGSLSPGVDRLAFSLFLDIDNCGDVKDRWIGRTVICSCCKLSYEHAQDIIDGLIDSDS 660

Query: 661  SKILG-NCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRDGALRLENSKIVYLYDEYGI 720
            SK LG N PQLH QF WLDVISSVKILHEISKTLK+KRFRDGALRLENSKIVYLYDEYGI
Sbjct: 661  SKNLGNNYPQLHGQFEWLDVISSVKILHEISKTLKKKRFRDGALRLENSKIVYLYDEYGI 720

Query: 721  PYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTYPNSALLRRQPEPILRKLREFESFCS 780
            PYDS FYEQKDSNFLVEEFMLLAN TVAEVISRT+P+SALLRR PEPILRKLREFESFCS
Sbjct: 721  PYDSAFYEQKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPILRKLREFESFCS 780

Query: 781  KHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYATRPMQLATYFCNGELKDGENG 840
            KHG ELDTSSSV+FQQSLEQIRIKLHDDP LF+I+ SYATRPMQLATYFC+GELKDGE G
Sbjct: 781  KHGFELDTSSSVQFQQSLEQIRIKLHDDPLLFDILTSYATRPMQLATYFCSGELKDGEKG 840

Query: 841  SHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEAEELYLKHQGIIPKVNSDEQMRCFTG 900
            SHYALA PLYTHFTSPLRRYPDI+VHRTLAAA+EAE+LYLKH+GI  KVNSDEQ+RCFTG
Sbjct: 841  SHYALAVPLYTHFTSPLRRYPDIIVHRTLAAAIEAEKLYLKHRGITQKVNSDEQIRCFTG 900

Query: 901  IYFDKDAADSSEGREALSSAASRHGLPCSKLLSDVALHCNNRKLASKHVADACNKLYMWA 960
            +YFDKDAADS EG+EALSSAA RHG+PC+KLL+DVALHCNNRKLASKHVAD C+KLYMWA
Sbjct: 901  MYFDKDAADSLEGKEALSSAALRHGVPCAKLLADVALHCNNRKLASKHVADGCDKLYMWA 960

Query: 961  LLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLEVEWLDATSTLVLSFFG 1020
            LLK+KKVLFSDARVLGLGPRFMS+YIQKLAIERRIYYDE EGL VEWL+ TSTLVLSFFG
Sbjct: 961  LLKKKKVLFSDARVLGLGPRFMSLYIQKLAIERRIYYDETEGLAVEWLETTSTLVLSFFG 1020

Query: 1021 TRRPHRNRGSSIKWKALEDVALVISPCDLNVKQRTLGGMSLSESGGAGTGGASVEQEPNL 1080
            TRR HR+RG SIKWKALEDVALV+SPCD NVKQR LG  S SE GG GTGGA VEQE NL
Sbjct: 1021 TRRSHRSRG-SIKWKALEDVALVVSPCDQNVKQRALGA-SPSELGGTGTGGAVVEQESNL 1080

Query: 1081 KSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYTSSYLK 1131
            KSHVSDTGIDPAVFPLTVRLLST+PVALHAVGGDDGPIDIGVRLY SSYLK
Sbjct: 1081 KSHVSDTGIDPAVFPLTVRLLSTLPVALHAVGGDDGPIDIGVRLYMSSYLK 1127

BLAST of Sed0025890.3 vs. NCBI nr
Match: XP_022938380.1 (DIS3-like exonuclease 2 [Cucurbita moschata])

HSP 1 Score: 1840.5 bits (4766), Expect = 0.0e+00
Identity = 926/1131 (81.87%), Postives = 1013/1131 (89.57%), Query Frame = 0

Query: 1    MRGAVEQSTPDRNDDGEKEKKKKRRYSRRSKHSASIATPVSCSSVNEISGEASECMENGR 60
            MRGAVEQSTP+R DDG+KEKKKKRR +RRSK +ASI+T VSCSSVN + GEASEC ENG+
Sbjct: 1    MRGAVEQSTPERYDDGDKEKKKKRRSNRRSKQNASISTSVSCSSVNGMPGEASECRENGK 60

Query: 61   IDANLTAPSNSSSPTQQACQSIHPSEPGLTGRNKISFGSLPPLHISKNAELSELQYSINQ 120
            I+ANLTAPSN SS TQQA +S HP E G+T RNKI+F SLPPLHIS+ AELSE Q  IN+
Sbjct: 61   INANLTAPSNHSSLTQQAHESNHPIEHGVTRRNKIAFSSLPPLHISEQAELSESQNLINE 120

Query: 121  DLYSLNTGGRIVKSCPEQIACGKMLQLSMSEHSPPADANATVNNTQRKYFTSHWAMDDVN 180
            +L+ L+ GG+ +KSCPEQI CG+M  +S+++HSPPAD   T NNTQRKYF SHW+++DV+
Sbjct: 121  NLHPLDAGGKTIKSCPEQIVCGRMPGISINQHSPPAD--VTENNTQRKYFASHWSVEDVD 180

Query: 181  ERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILINGIASQNRALEGDIVAIMVDPFTS 240
            E LQKG IF+A FRVN+HNRLEAYCKIDGLPVD+LINGIASQNRA+EGDIVAIMVDP TS
Sbjct: 181  EGLQKGDIFRAFFRVNSHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIMVDPLTS 240

Query: 241  WTMMKGTCEAHNNKHSMEDANLPAEETEKNSHNCKDKNKVNSDVKSDSYRSSSSSDKRCC 300
            WT MKGT E HN+ HSMEDANLPAE TE +  NCK KNK+++ VKSDS+RSSSS DKRCC
Sbjct: 241  WTRMKGTSETHNSTHSMEDANLPAEATENDGRNCKGKNKLDASVKSDSFRSSSSPDKRCC 300

Query: 301  SEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHYSSIQDDVSMAIGRICTLINLYPSK 360
            SE+K+LDGTACDDLL   E  D Y  S V P +AHYS  QDDVS AI RIC +I+ YP K
Sbjct: 301  SEDKILDGTACDDLLLKNEQRDVYQSSVVDPPEAHYSRNQDDVSKAIQRICAVISSYPGK 360

Query: 361  RPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYTKEMKTKSCLSSSHDSGYVQLMPND 420
            RPTGRVVAI+EKSR RE+IVGHLNVKKFLSF EIY KEM TKSCLS SH+ GYVQLMPND
Sbjct: 361  RPTGRVVAILEKSRQRESIVGHLNVKKFLSFQEIYMKEMNTKSCLSPSHNCGYVQLMPND 420

Query: 421  ARFPIMIVLARDLPDCIKKRLENGDVTVESELVAARIDEWVEESSAPRAQVLHVLGQGSK 480
            ARFPIM+VLA DLPD IKKRL+NGDVTVE+ELVA +I EWV+ESSAP+A VLHVLG+GS+
Sbjct: 421  ARFPIMMVLAGDLPDSIKKRLDNGDVTVENELVAVKIHEWVKESSAPQALVLHVLGRGSE 480

Query: 481  IESHIDAILFENAIRKCEFSQDSLSCLPRTPWKIPHEELQCRRDLRNLCIFTIDPSSVLD 540
            + SHIDAILFENAI  CEFS DSL+CLP TPWKIPHEELQCRRDLRNLCIFTIDPSS  D
Sbjct: 481  VASHIDAILFENAIHSCEFSNDSLACLPHTPWKIPHEELQCRRDLRNLCIFTIDPSSASD 540

Query: 541  LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQHKIPMLPPLLS 600
            LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQ KIPMLPPLLS
Sbjct: 541  LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS 600

Query: 601  ENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSCCKLSYEHAQDIIDGLIDSDS 660
            +N+ SL+PGVDRLAFSLFLD+D+ G VKD WI RTVICSCCKLSYEHAQDIIDGLIDSD+
Sbjct: 601  KNVGSLSPGVDRLAFSLFLDIDNSGDVKDRWIGRTVICSCCKLSYEHAQDIIDGLIDSDN 660

Query: 661  SKILG-NCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRDGALRLENSKIVYLYDEYGI 720
             K LG N PQLH QF+W DVISSVK+LHEISKTLK+KRFRDGALRLENSKIVYLYDEYGI
Sbjct: 661  LKNLGNNYPQLHGQFAWPDVISSVKLLHEISKTLKKKRFRDGALRLENSKIVYLYDEYGI 720

Query: 721  PYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTYPNSALLRRQPEPILRKLREFESFCS 780
            PYDSTFYEQKDSNFLVEEFMLLAN TVAEVISRT+P+SALLRR PEPILRKLREFESFCS
Sbjct: 721  PYDSTFYEQKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPILRKLREFESFCS 780

Query: 781  KHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYATRPMQLATYFCNGELKDGENG 840
            KHG ELDTSSSV+FQQSLEQIRIKLHDDP LF+I+ SYATRPMQLATYFC+GELKDGE G
Sbjct: 781  KHGFELDTSSSVQFQQSLEQIRIKLHDDPLLFDILTSYATRPMQLATYFCSGELKDGEKG 840

Query: 841  SHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEAEELYLKHQGIIPKVNSDEQMRCFTG 900
            SHYALA PLYTHFTSPLRRYPDIVVHRTLAAA+EAE+LYLKH+GII KVNSDEQ+RCFTG
Sbjct: 841  SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKLYLKHRGIIQKVNSDEQIRCFTG 900

Query: 901  IYFDKDAADSSEGREALSSAASRHGLPCSKLLSDVALHCNNRKLASKHVADACNKLYMWA 960
            +YFDKDAADS EGREALSSAA RHG+PC+KLL+DVALHCN+RKLASKHVAD C+KLYMWA
Sbjct: 901  MYFDKDAADSLEGREALSSAALRHGVPCAKLLADVALHCNDRKLASKHVADGCDKLYMWA 960

Query: 961  LLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLEVEWLDATSTLVLSFFG 1020
            +LK+KKVLFSDARVLGLGPRFMS+YIQKLAIERRIYYDE EGL VEWL+ TSTLVLSFFG
Sbjct: 961  VLKKKKVLFSDARVLGLGPRFMSLYIQKLAIERRIYYDETEGLAVEWLETTSTLVLSFFG 1020

Query: 1021 TRRPHRNRGSSIKWKALEDVALVISPCDLNVKQRTLGGMSLSESGGAGTGGASVEQEPNL 1080
            TRR HR+RG SIKWKALEDVALV+SPCD NVKQR L G+S SE GG GTGGA VEQE NL
Sbjct: 1021 TRRSHRSRG-SIKWKALEDVALVVSPCDHNVKQRAL-GVSPSELGGTGTGGAVVEQESNL 1080

Query: 1081 KSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYTSSYLK 1131
            KSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLY SSYLK
Sbjct: 1081 KSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYMSSYLK 1127

BLAST of Sed0025890.3 vs. ExPASy Swiss-Prot
Match: P0DM58 (DIS3-like exonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=SOV PE=1 SV=1)

HSP 1 Score: 962.6 bits (2487), Expect = 3.9e-279
Identity = 553/1134 (48.77%), Postives = 726/1134 (64.02%), Query Frame = 0

Query: 1    MRGAVEQSTPDRNDDGEKEKKKK-RRYSRRSKHSASIATPVSCSSVNEISGEASECMENG 60
            M+ A  + + +R ++G K+K+ + ++ +RRSK S   + P+  + V E          +G
Sbjct: 1    MKSASSEQSVERIENGHKKKRNRPQKQNRRSKQS---SVPIEDAHVEE--------SLDG 60

Query: 61   RIDANLTAPSNSSSPTQQACQSIHPSEPGLTGRNKISFGSLPPLHISKNAELSELQYSIN 120
            R  +   A  ++SS  QQ   +    E      + ++F S+PP+     AE    + S +
Sbjct: 61   RDSSRSKAKDSTSSSKQQRPNT---DELEAMRASNVAFNSMPPM----RAESGYPRRSAS 120

Query: 121  QDLYSLNTGGRIV-KSCPEQIACGKMLQLSMSEHSPPADA---NATVNNTQRKYFTSHWA 180
              L S     +++ KSCP+  AC         E SP  +         ++QRK F+SHW+
Sbjct: 121  PLLSSPEVSKQLLSKSCPDPRAC---------EQSPGMNGELFQQIEGSSQRKIFSSHWS 180

Query: 181  MDDVNERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILINGIASQNRALEGDIVAIMV 240
            +D V E L+KG  FKALFRVNAHNR EAYCKIDG+P DILING   Q+RA+EGD V I +
Sbjct: 181  LDAVTEALEKGEAFKALFRVNAHNRNEAYCKIDGVPTDILINGNVCQSRAVEGDTVVIKL 240

Query: 241  DPFTSWTMMKGTCEAHNNKHSMEDANLPAEETEKNSHNCKDKNKVNSDVKSDSYRSSSSS 300
            DP + W  MKG       K   E  N P E+ +K +      + V       S   SS  
Sbjct: 241  DPLSLWPKMKGFVTESAAK--PEGTNSPPEKDDKKARQKNGIDVVEGFEDGFSKNKSSVI 300

Query: 301  DKRCCSEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHYSSIQDDVSMAIGRICTLIN 360
             K   +        + D  L +  +C+                 Q     A+ ++C +++
Sbjct: 301  GKGAKNGVTPSSPPSLDSCLGS--FCE-----------------QKGNCSAVDKLCGILS 360

Query: 361  LYPSKRPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYTKEMKTKSCLSSSHDSGYVQ 420
             +P KRPTG+VVA++EKS  R++IVG L+VK ++ + E   K  K+   LS   D  YVQ
Sbjct: 361  SFPHKRPTGQVVAVVEKSLVRDSIVGLLDVKGWIHYKESDPKRCKSPLSLS---DDEYVQ 420

Query: 421  LMPNDARFPIMIVLARDLPDCIKKRLENGDVTVESELVAARIDEWVEESSAPRAQVLHVL 480
            LMP D RFP +IV    LP  I+ RLEN D  +E+ELVAA+I +W E S  P AQ+ H+ 
Sbjct: 421  LMPADPRFPKLIVPFHVLPGSIRARLENLDPNLEAELVAAQIVDWGEGSPFPVAQITHLF 480

Query: 481  GQGSKIESHIDAILFENAIRKCEFSQDSLSCLPRTPWKIPHEELQCRRDLRNLCIFTIDP 540
            G+GS++E  I+AIL++N++   +FS  SL+ LPR PW++P EE+Q R+DLR+LC+ TIDP
Sbjct: 481  GRGSELEPQINAILYQNSVCDSDFSPGSLTSLPRVPWEVPEEEVQRRKDLRDLCVLTIDP 540

Query: 541  SSVLDLDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQHKIPML 600
            S+  DLDDALSVQ L  G FRVG+HIADVS+FVLP+TALD EA+ RSTSVYL+Q KI ML
Sbjct: 541  STATDLDDALSVQSLPGGFFRVGVHIADVSYFVLPETALDTEARFRSTSVYLMQRKISML 600

Query: 601  PPLLSENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSCCKLSYEHAQDIIDGL 660
            PPLLSEN+ SL+PG DRLAFS+  DL+  G V D WI RT+I SCCKLSY+HAQDIIDG 
Sbjct: 601  PPLLSENVGSLSPGADRLAFSILWDLNREGDVIDRWIGRTIIRSCCKLSYDHAQDIIDGK 660

Query: 661  IDSDSSKILGNCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRDGALRLENSKIVYLYD 720
             D   +      P LH  F W DV  SVK L EIS TL++KRFR+GAL+LENSK V+L+D
Sbjct: 661  SDVAEN----GWPALHGSFKWCDVTRSVKQLSEISTTLRQKRFRNGALQLENSKPVFLFD 720

Query: 721  EYGIPYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTYPNSALLRRQPEPILRKLREFE 780
            E+G+PYD     +K SNFLVEEFMLLAN T AEVIS+ YP S+LLRR PEP  RKL+EFE
Sbjct: 721  EHGVPYDFVTCSRKGSNFLVEEFMLLANMTAAEVISQAYPASSLLRRHPEPNTRKLKEFE 780

Query: 781  SFCSKHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYATRPMQLATYFCNGELKD 840
             FCSKHG++LD SSS + Q SLE+I   L DD    +I+ +YA +PMQLA+YFC G LKD
Sbjct: 781  GFCSKHGMDLDISSSGQLQDSLEKITGNLKDDSVFVDILNNYAIKPMQLASYFCTGNLKD 840

Query: 841  G-ENGSHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEAEELYLKHQGIIPKVNSDEQM 900
                  HYALA PLYTHFTSPLRRYPDIVVHR LAAALEAEELY K +    +   DE  
Sbjct: 841  SVAEWGHYALAVPLYTHFTSPLRRYPDIVVHRALAAALEAEELYSKQK----QTAIDEGR 900

Query: 901  RCFTGIYFDKDAADSSEGREALSSAASRHGLPCSKLLSDVALHCNNRKLASKHVADACNK 960
             CFTGI+F+KDAA+S EG+EALS AA +HG+P +++LSDVA +CN RKLA++ V DAC+K
Sbjct: 901  SCFTGIHFNKDAAESIEGKEALSVAALKHGVPSTEILSDVAAYCNERKLAARKVRDACDK 960

Query: 961  LYMWALLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLEVEWLDATSTLV 1020
            LY W +LK+K++   +ARV+ LG RFM+VYI KL IERRIYYD++EGL  +WL+ATSTL+
Sbjct: 961  LYTWFVLKQKEIFPCEARVMNLGSRFMTVYISKLGIERRIYYDQIEGLCADWLEATSTLI 1020

Query: 1021 LSFFGTRRPHRNRGSSIKWKALEDVALVISPCDLNVKQRTLGGMSLSESGGAGTGGASVE 1080
            +      + +  RG    +K +++   ++SPC++ V + +   +  +ES  A        
Sbjct: 1021 VD-----KLYSKRGGRGFFKPMKEAVYLVSPCEVCVAKCSALSVHDTESPEA-------- 1054

Query: 1081 QEPNLKSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYTSSY 1129
                    VS   + PAVFPLT++L STIPV LHAVGGDDGP+DIG RLY SSY
Sbjct: 1081 --------VSIDEVAPAVFPLTIQLFSTIPVVLHAVGGDDGPLDIGARLYMSSY 1054

BLAST of Sed0025890.3 vs. ExPASy Swiss-Prot
Match: Q0WPN0 (Inactive exonuclease DIS3L2 OS=Arabidopsis thaliana OX=3702 GN=SOV PE=2 SV=1)

HSP 1 Score: 959.1 bits (2478), Expect = 4.3e-278
Identity = 552/1134 (48.68%), Postives = 725/1134 (63.93%), Query Frame = 0

Query: 1    MRGAVEQSTPDRNDDGEKEKKKK-RRYSRRSKHSASIATPVSCSSVNEISGEASECMENG 60
            M+ A  + + +R ++G K+K+ + ++ +RRSK S   + P+  + V E          +G
Sbjct: 1    MKSASSEQSVERIENGHKKKRNRPQKQNRRSKQS---SVPIEDAHVEE--------SLDG 60

Query: 61   RIDANLTAPSNSSSPTQQACQSIHPSEPGLTGRNKISFGSLPPLHISKNAELSELQYSIN 120
            R  +   A  ++SS  QQ   +    E      + ++F S+PP+     AE    + S +
Sbjct: 61   RDSSRSKAKDSTSSSKQQRPNT---DELEAMRASNVAFNSMPPM----RAESGYPRRSAS 120

Query: 121  QDLYSLNTGGRIV-KSCPEQIACGKMLQLSMSEHSPPADA---NATVNNTQRKYFTSHWA 180
              L S     +++ KSCP+  AC         E SP  +         ++QRK F+SHW+
Sbjct: 121  PLLSSPEVSKQLLSKSCPDPRAC---------EQSPGMNGELFQQIEGSSQRKIFSSHWS 180

Query: 181  MDDVNERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILINGIASQNRALEGDIVAIMV 240
            +D V E L+KG  FKALFRVNAHNR EAYCKIDG+P DILING   Q+RA+EGD V I +
Sbjct: 181  LDAVTEALEKGEAFKALFRVNAHNRNEAYCKIDGVPTDILINGNVCQSRAVEGDTVVIKL 240

Query: 241  DPFTSWTMMKGTCEAHNNKHSMEDANLPAEETEKNSHNCKDKNKVNSDVKSDSYRSSSSS 300
            DP + W  MKG       K   E  N P E+ +K +      + V       S   SS  
Sbjct: 241  DPLSLWPKMKGFVTESAAK--PEGTNSPPEKDDKKARQKNGIDVVEGFEDGFSKNKSSVI 300

Query: 301  DKRCCSEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHYSSIQDDVSMAIGRICTLIN 360
             K   +        + D  L +  +C+                 Q     A+ ++C +++
Sbjct: 301  GKGAKNGVTPSSPPSLDSCLGS--FCE-----------------QKGNCSAVDKLCGILS 360

Query: 361  LYPSKRPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYTKEMKTKSCLSSSHDSGYVQ 420
             +P KRPTG+VVA++EKS  R++IVG L+VK ++ + E   K  K+   LS   D  YVQ
Sbjct: 361  SFPHKRPTGQVVAVVEKSLVRDSIVGLLDVKGWIHYKESDPKRCKSPLSLS---DDEYVQ 420

Query: 421  LMPNDARFPIMIVLARDLPDCIKKRLENGDVTVESELVAARIDEWVEESSAPRAQVLHVL 480
            LMP D RFP +IV    LP  I+ RLEN D  +E+ELVAA+I +W E S  P AQ+ H+ 
Sbjct: 421  LMPADPRFPKLIVPFHVLPGSIRARLENLDPNLEAELVAAQIVDWGEGSPFPVAQITHLF 480

Query: 481  GQGSKIESHIDAILFENAIRKCEFSQDSLSCLPRTPWKIPHEELQCRRDLRNLCIFTIDP 540
            G+GS++E  I+AIL++N++   +FS  SL+ LPR PW++P EE+Q R+DLR+LC+ TIDP
Sbjct: 481  GRGSELEPQINAILYQNSVCDSDFSPGSLTSLPRVPWEVPEEEVQRRKDLRDLCVLTIDP 540

Query: 541  SSVLDLDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQHKIPML 600
            S+  DLDDALSVQ L  G FRVG+HIADVS+FVLP+TALD EA+ RSTSVYL+Q KI ML
Sbjct: 541  STATDLDDALSVQSLPGGFFRVGVHIADVSYFVLPETALDTEARFRSTSVYLMQRKISML 600

Query: 601  PPLLSENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSCCKLSYEHAQDIIDGL 660
            PPLLSEN+ SL+PG DRLAFS+  DL+  G V D WI RT+I SCCKLSY+HAQDIIDG 
Sbjct: 601  PPLLSENVGSLSPGADRLAFSILWDLNREGDVIDRWIGRTIIRSCCKLSYDHAQDIIDGK 660

Query: 661  IDSDSSKILGNCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRDGALRLENSKIVYLYD 720
             D   +      P LH  F W DV  SVK L EIS TL++KRFR+GAL+LENSK V+L+D
Sbjct: 661  SDVAEN----GWPALHGSFKWCDVTRSVKQLSEISTTLRQKRFRNGALQLENSKPVFLFD 720

Query: 721  EYGIPYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTYPNSALLRRQPEPILRKLREFE 780
            E+G+PYD     +K SNFLVEEFMLLAN T AEVIS+ Y  S+LLRR PEP  RKL+EFE
Sbjct: 721  EHGVPYDFVTCSRKGSNFLVEEFMLLANMTAAEVISQAYRASSLLRRHPEPNTRKLKEFE 780

Query: 781  SFCSKHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYATRPMQLATYFCNGELKD 840
             FCSKHG++LD SSS + Q SLE+I   L DD    +I+ +YA +PMQLA+YFC G LKD
Sbjct: 781  GFCSKHGMDLDISSSGQLQDSLEKITGNLKDDSVFVDILNNYAIKPMQLASYFCTGNLKD 840

Query: 841  G-ENGSHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEAEELYLKHQGIIPKVNSDEQM 900
                  HYALA PLYTHFTSPLRRYPDIVVHR LAAALEAEELY K +    +   DE  
Sbjct: 841  SVAEWGHYALAVPLYTHFTSPLRRYPDIVVHRALAAALEAEELYSKQK----QTAIDEGR 900

Query: 901  RCFTGIYFDKDAADSSEGREALSSAASRHGLPCSKLLSDVALHCNNRKLASKHVADACNK 960
             CFTGI+F+KDAA+S EG+EALS AA +HG+P +++LSDVA +CN RKLA++ V DAC+K
Sbjct: 901  SCFTGIHFNKDAAESIEGKEALSVAALKHGVPSTEILSDVAAYCNERKLAARKVRDACDK 960

Query: 961  LYMWALLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLEVEWLDATSTLV 1020
            LY W +LK+K++   +ARV+ LG RFM+VYI KL IERRIYYD++EGL  +WL+ATSTL+
Sbjct: 961  LYTWFVLKQKEIFPCEARVMNLGSRFMTVYISKLGIERRIYYDQIEGLCADWLEATSTLI 1020

Query: 1021 LSFFGTRRPHRNRGSSIKWKALEDVALVISPCDLNVKQRTLGGMSLSESGGAGTGGASVE 1080
            +      + +  RG    +K +++   ++SPC++ V + +   +  +ES  A        
Sbjct: 1021 VD-----KLYSKRGGRGFFKPMKEAVYLVSPCEVCVAKCSALSVHDTESPEA-------- 1054

Query: 1081 QEPNLKSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYTSSY 1129
                    VS   + PAVFPLT++L STIPV LHAVGGDDGP+DIG RLY SSY
Sbjct: 1081 --------VSIDEVAPAVFPLTIQLFSTIPVVLHAVGGDDGPLDIGARLYMSSY 1054

BLAST of Sed0025890.3 vs. ExPASy Swiss-Prot
Match: Q8IYB7 (DIS3-like exonuclease 2 OS=Homo sapiens OX=9606 GN=DIS3L2 PE=1 SV=4)

HSP 1 Score: 419.5 bits (1077), Expect = 1.2e-115
Identity = 286/841 (34.01%), Postives = 420/841 (49.94%), Query Frame = 0

Query: 159 NATVNNTQRKYFTSHWAMDDVNERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILING 218
           N +    ++  F ++ + +DV+E L++G + + + R+N     EA+        DI I+G
Sbjct: 39  NRSTRGKKKSIFETYMSKEDVSEGLKRGTLIQGVLRINPKKFHEAFIPSPDGDRDIFIDG 98

Query: 219 IASQNRALEGDIVAIMVDPFTSWTMMKGTCEAHNNKHSMEDANLPAEETEKN--SHNCKD 278
           + ++NRAL GD+V + + P   W ++K        + + E +++P E    +    + K 
Sbjct: 99  VVARNRALNGDLVVVKLLPEEHWKVVKPESNDKETEAAYE-SDIPEELCGHHLPQQSLKS 158

Query: 279 KNKVNSDVKSDSYRSSSSSDKRCCSEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHY 338
            N     +    +  S S D    ++  ++DG      +     C +      G      
Sbjct: 159 YNDSPDVIVEAQFDGSDSEDGHGITQNVLVDG------VKKLSVCVSEKGREDGD----- 218

Query: 339 SSIQDDVSMAIGRICTLINLYPSKRPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYT 398
           + +  D +  I +    ++   S + + +VV I+EK   R                   T
Sbjct: 219 APVTKDETTCISQDTRALS-EKSLQRSAKVVYILEKKHSRAA-----------------T 278

Query: 399 KEMKTKSCLSSSHDSGYVQLMPNDARFPIMIVLARDLPDCIKKRLENGDVTVESELVAAR 458
             +K  +  +S     Y    P+D R P + V  +D P     R ++      + L   R
Sbjct: 279 GFLKLLADKNSELFRKYALFSPSDHRVPRIYVPLKDCPQDFVARPKD----YANTLFICR 338

Query: 459 IDEWVEESSAPRAQVLHVLGQGSKIESHIDAILFENAIRKCEFSQDSLSCLPR-TPWKIP 518
           I +W E+ +    Q+   LGQ  +IE   + IL E  +   +FS + L CLP+  PW IP
Sbjct: 339 IVDWKEDCNFALGQLAKSLGQAGEIEPETEGILTEYGVDFSDFSSEVLECLPQGLPWTIP 398

Query: 519 HEELQCRRDLRNLCIFTIDPSSVLDLDDALSVQKLANGIFRVGIHIADVSHFVLPDTALD 578
            EE   RRDLR  CIFTIDPS+  DLDDALS + LA+G F+VG+HIADVS+FV   + LD
Sbjct: 399 PEEFSKRRDLRKDCIFTIDPSTARDLDDALSCKPLADGNFKVGVHIADVSYFVPEGSDLD 458

Query: 579 KEAQIRSTSVYLLQHKIPMLPPLLSENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRT 638
           K A  R+TSVYL+Q  +PMLP LL E + SLNP  D+L FS+   L   G + D W  RT
Sbjct: 459 KVAAERATSVYLVQKVVPMLPRLLCEELCSLNPMSDKLTFSVIWTLTPEGKILDEWFGRT 518

Query: 639 VICSCCKLSYEHAQDIIDGLIDSDSSKILGNCPQLHSQFSWLDVISSVKILHEISKTLKE 698
           +I SC KLSYEHAQ +I+   +   +K L   P +  + S  +V  +V  LH I+K L++
Sbjct: 519 IIRSCTKLSYEHAQSMIESPTEKIPAKEL---PPISPEHSSEEVHQAVLNLHGIAKQLRQ 578

Query: 699 KRFRDGALRLENSKIVYLYD-EYGIPYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTY 758
           +RF DGALRL+  K+ +  D E G+P     YE ++SN LVEEFMLLAN  VA  I R +
Sbjct: 579 QRFVDGALRLDQLKLAFTLDHETGLPQGCHIYEYRESNKLVEEFMLLANMAVAHKIHRAF 638

Query: 759 PNSALLRRQPEPILRKLREFESFCSKHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIV 818
           P  ALLRR P P  R L +   FC + GL +D SS+    +SL Q         +   ++
Sbjct: 639 PEQALLRRHPPPQTRMLSDLVEFCDQMGLPVDFSSAGALNKSLTQTFGDDKYSLARKEVL 698

Query: 819 ISYATRPMQLATYFCNGELKDGENGSHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEA 878
            +  +RPMQ+A YFC+G L+D     HYAL  PLYTHFTSP+RR+ D++VHR LAAAL  
Sbjct: 699 TNMCSRPMQMALYFCSGLLQDPAQFRHYALNVPLYTHFTSPIRRFADVLVHRLLAAALGY 758

Query: 879 EELYLKHQGIIPKVNSDEQMRCFTGIYFDKDAADSSEGREALSSAASRHGLPCSKLLSDV 938
                                                 RE L  A           L   
Sbjct: 759 --------------------------------------RERLDMAPD--------TLQKQ 796

Query: 939 ALHCNNRKLASKHVADACNKLYMWALLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERRI 996
           A HCN+R++ASK V +    L+   L+KE   L S+A V+G+  +   V + +  +++RI
Sbjct: 819 ADHCNDRRMASKRVQELSTSLFFAVLVKESGPLESEAMVMGILKQAFDVLVLRYGVQKRI 796

BLAST of Sed0025890.3 vs. ExPASy Swiss-Prot
Match: Q8CI75 (DIS3-like exonuclease 2 OS=Mus musculus OX=10090 GN=Dis3l2 PE=1 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 2.7e-115
Identity = 285/842 (33.85%), Postives = 421/842 (50.00%), Query Frame = 0

Query: 159 NATVNNTQRKYFTSHWAMDDVNERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILING 218
           N ++   ++  F ++ + +DV+E L++G + + + R+N     EA+        DI I+G
Sbjct: 39  NKSMRGKKKSIFETYMSKEDVSEGLKRGTLIQGVLRINPKKFHEAFIPSPDGDRDIFIDG 98

Query: 219 IASQNRALEGDIVAIMVDPFTSWTMMKGTCEAHNNKHSME---DANLPAEETEKNSHNCK 278
           + ++NRAL GD+V + + P   W  +K      +N   +E   +A++P E    +     
Sbjct: 99  VVARNRALNGDLVVVKLLPEDQWKAVK----PESNDKEIEATYEADIPEEGCGHHPLQQS 158

Query: 279 DKNKVNSDVKSDSYRSSSSSDKRCCSEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAH 338
            K     DV  ++    S S+ R  +   ++DG      LS          S+    +  
Sbjct: 159 RKGWSGPDVIIEAQFDDSDSEDRHGNTSGLVDGV---KKLSISTPDRGKEDSSTPVMKDE 218

Query: 339 YSSIQDDVSMAIGRICTLINLYPSKRPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIY 398
            + I  D      +         S + + +VV I+EK   R                   
Sbjct: 219 NTPIPQDTRGLSEK---------SLQKSAKVVYILEKKHSRAA----------------- 278

Query: 399 TKEMKTKSCLSSSHDSGYVQLMPNDARFPIMIVLARDLPDCIKKRLENGDVTVESELVAA 458
           T  +K  +  +S     Y    P+D R P + V  +D P     R ++      + L   
Sbjct: 279 TGILKLLADKNSDLFKKYALFSPSDHRVPRIYVPLKDCPQDFMTRPKD----FANTLFIC 338

Query: 459 RIDEWVEESSAPRAQVLHVLGQGSKIESHIDAILFENAIRKCEFSQDSLSCLPRT-PWKI 518
           RI +W E+ +    Q+   LGQ  +IE   + IL E  +   +FS + L CLP++ PW I
Sbjct: 339 RIIDWKEDCNFALGQLAKSLGQAGEIEPETEGILTEYGVDFSDFSSEVLECLPQSLPWTI 398

Query: 519 PHEELQCRRDLRNLCIFTIDPSSVLDLDDALSVQKLANGIFRVGIHIADVSHFVLPDTAL 578
           P +E+  RRDLR  CIFTIDPS+  DLDDAL+ ++L +G F VG+HIADVS+FV   ++L
Sbjct: 399 PPDEVGKRRDLRKDCIFTIDPSTARDLDDALACRRLTDGTFEVGVHIADVSYFVPEGSSL 458

Query: 579 DKEAQIRSTSVYLLQHKIPMLPPLLSENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDR 638
           DK A  R+TSVYL+Q  +PMLP LL E + SLNP  D+L FS+   L   G + + W  R
Sbjct: 459 DKVAAERATSVYLVQKVVPMLPRLLCEELCSLNPMTDKLTFSVIWKLTPEGKILEEWFGR 518

Query: 639 TVICSCCKLSYEHAQDIIDGLIDSDSSKILGNCPQLHSQFSWLDVISSVKILHEISKTLK 698
           T+I SC KLSY+HAQ +I+   +    + L   P +  + S  +V  +V  LH I+K L+
Sbjct: 519 TIIRSCTKLSYDHAQSMIENPTEKIPEEEL---PPISPEHSVEEVHQAVLNLHSIAKQLR 578

Query: 699 EKRFRDGALRLENSKIVYLYD-EYGIPYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRT 758
            +RF DGALRL+  K+ +  D E G+P     YE +DSN LVEEFMLLAN  VA  I RT
Sbjct: 579 RQRFVDGALRLDQLKLAFTLDHETGLPQGCHIYEYRDSNKLVEEFMLLANMAVAHKIFRT 638

Query: 759 YPNSALLRRQPEPILRKLREFESFCSKHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNI 818
           +P  ALLRR P P  + L +   FC + GL +D SS+    +SL +         +   +
Sbjct: 639 FPEQALLRRHPPPQTKMLSDLVEFCDQMGLPMDVSSAGALNKSLTKTFGDDKYSLARKEV 698

Query: 819 VISYATRPMQLATYFCNGELKDGENGSHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALE 878
           + +  +RPMQ+A YFC+G L+D E   HYAL  PLYTHFTSP+RR+ D++VHR LAAAL 
Sbjct: 699 LTNMYSRPMQMALYFCSGMLQDQEQFRHYALNVPLYTHFTSPIRRFADVIVHRLLAAALG 758

Query: 879 AEELYLKHQGIIPKVNSDEQMRCFTGIYFDKDAADSSEGREALSSAASRHGLPCSKLLSD 938
             E         P V  D                                       L  
Sbjct: 759 YSEQ--------PDVEPD--------------------------------------TLQK 794

Query: 939 VALHCNNRKLASKHVADACNKLYMWALLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERR 996
            A HCN+R++ASK V +    L+   L+KE   L S+A V+G+  +   V + +  +++R
Sbjct: 819 QADHCNDRRMASKRVQELSIGLFFAVLVKESGPLESEAMVMGVLNQAFDVLVLRFGVQKR 794

BLAST of Sed0025890.3 vs. ExPASy Swiss-Prot
Match: Q0V9R3 (DIS3-like exonuclease 2 OS=Xenopus tropicalis OX=8364 GN=dis3l2 PE=2 SV=2)

HSP 1 Score: 401.4 bits (1030), Expect = 3.5e-110
Identity = 275/836 (32.89%), Postives = 401/836 (47.97%), Query Frame = 0

Query: 166 QRKYFTSHWAMDDVNERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILINGIASQNRA 225
           ++  F ++   ++V+  L++G + +   R+N     EAY        D+ I+G+  +NRA
Sbjct: 33  KKSVFEAYMTKEEVSAGLKRGELIQGPLRINPKKFHEAYLPSPDGVRDLFIDGVVPRNRA 92

Query: 226 LEGDIVAIMVDPFTSWTMMKGTCEAHNNKHSMEDANLPAEETEKNSH----NCKDKNKVN 285
           L GD+V + + P   W ++K            ED + P   T    H    +    +  N
Sbjct: 93  LNGDVVVVKLLPQEQWKVLKNDV--------CEDDDTPGHSTGNKQHALSPHLMKSSAKN 152

Query: 286 SDVKSDSYRSSSSSDKRCCSEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHYSSIQD 345
            D+  ++   SS+ D      E  L G    + + + +   A         Q    +  D
Sbjct: 153 PDLIIEAKVDSSAED----GHESALIGCLQKE-IKDQDKLGAIEEKT--SKQGDPKTFSD 212

Query: 346 DVSMAIGRICTLINLYPSKRPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYTKEMKT 405
           D      ++  ++    S+  TG +  + +KS                        ++  
Sbjct: 213 DCFQKTAKVVYILEKKHSRAATGFIKPLSDKS-----------------------SDLAR 272

Query: 406 KSCLSSSHDSGYVQLMPNDARFPIMIVLARDLPDCIKKRLENGDVTVESELVAARIDEWV 465
           K  L S          P D R P + V   D P       E    T  + L    I  W 
Sbjct: 273 KRALFS----------PVDHRLPRIYVPLGDCPHDFAIHPE----TYANTLFICSITAWR 332

Query: 466 EESSAPRAQVLHVLGQGSKIESHIDAILFENAIRKCEFSQDSLSCLPR-TPWKIPHEELQ 525
           ++S+    +++  LGQ  +IE   + IL E  +   +F    L CLP+  PW IP EE Q
Sbjct: 333 DDSNFAEGKLMKSLGQAGEIEPETEGILVEYGVDFSDFPDKVLQCLPQDLPWTIPQEEFQ 392

Query: 526 CRRDLRNLCIFTIDPSSVLDLDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQI 585
            R+DLRN CIFTIDP++  DLDDALS + L +G F VG+HIADVS+FV   +ALD  A  
Sbjct: 393 KRKDLRNECIFTIDPATARDLDDALSCKPLPDGNFEVGVHIADVSYFVAEGSALDIMASE 452

Query: 586 RSTSVYLLQHKIPMLPPLLSENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSC 645
           R+TSVYL+Q  IPMLP LL E + SLNP  DRL FS+   +   G + D W  R+VICSC
Sbjct: 453 RATSVYLVQKVIPMLPRLLCEELCSLNPMTDRLTFSVIWKITPQGEILDEWFGRSVICSC 512

Query: 646 CKLSYEHAQDIIDGLIDSDSSKILGNCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRD 705
            KLSY+HAQ++I+     D        P +  Q +  ++  +V  LH I++ L+++RF D
Sbjct: 513 VKLSYDHAQNMIN---HPDKKIEQHELPPVSPQHTINEIHQAVLNLHLIAQNLRKQRFDD 572

Query: 706 GALRLENSKIVYLYD-EYGIPYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTYPNSAL 765
           GALRL+  K+ +  D E G+P     Y+ +DSN LVEEFMLLAN  VA  I R +P  AL
Sbjct: 573 GALRLDQLKLTFTLDKESGLPQGCYIYQYRDSNKLVEEFMLLANMAVAHHIYRRFPEEAL 632

Query: 766 LRRQPEPILRKLREFESFCSKHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYAT 825
           LRR P P  + L +   FC + GL+LD SSS    +SL           +   ++ +  +
Sbjct: 633 LRRHPPPQTKMLNDLIEFCDQMGLQLDFSSSGTLHKSLNDQFETDEYSAARKEVLTNMCS 692

Query: 826 RPMQLATYFCNGELKDGENGSHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEAEELYL 885
           RPMQ+A YFC G LKD     HYAL  PLYTHFTSP+RR+ D++VHR LAA+L       
Sbjct: 693 RPMQMAVYFCTGALKDETLFHHYALNVPLYTHFTSPIRRFADVIVHRLLAASL------- 752

Query: 886 KHQGIIPKVNSDEQMRCFTGIYFDKDAADSSEGREALSSAASRHGLPCSKLLSDVALHCN 945
              G  P +   ++                                    ++   A HCN
Sbjct: 753 ---GCGPPLKMPKE------------------------------------VIQKQADHCN 767

Query: 946 NRKLASKHVADACNKLYMWALLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERRIY 996
           +RK ASK V +   +L+    +KE   L S+A V+G+      V + +  +++RIY
Sbjct: 813 DRKTASKRVQELSAELFFSVFVKECGPLESEAMVMGVLNEAFDVIVLRFGVQKRIY 767

BLAST of Sed0025890.3 vs. ExPASy TrEMBL
Match: A0A6J1JX50 (DIS3-like exonuclease 2 OS=Cucurbita maxima OX=3661 GN=LOC111489125 PE=3 SV=1)

HSP 1 Score: 1848.9 bits (4788), Expect = 0.0e+00
Identity = 930/1131 (82.23%), Postives = 1011/1131 (89.39%), Query Frame = 0

Query: 1    MRGAVEQSTPDRNDDGEKEKKKKRRYSRRSKHSASIATPVSCSSVNEISGEASECMENGR 60
            MRGAVEQSTP+R DDG+KEKKKKRR +RRSK +ASI+T VSCSSVN +SGEASECMENG+
Sbjct: 1    MRGAVEQSTPERYDDGDKEKKKKRRSNRRSKQNASISTSVSCSSVNGMSGEASECMENGK 60

Query: 61   IDANLTAPSNSSSPTQQACQSIHPSEPGLTGRNKISFGSLPPLHISKNAELSELQYSINQ 120
            IDANLTAPSN SS TQQA +S HP E G+T RNKI+F SLPPLHIS+ AELSE Q  IN+
Sbjct: 61   IDANLTAPSNHSSLTQQAHESNHPIEHGVTRRNKIAFSSLPPLHISEQAELSESQNLINE 120

Query: 121  DLYSLNTGGRIVKSCPEQIACGKMLQLSMSEHSPPADANATVNNTQRKYFTSHWAMDDVN 180
            +L+ L+ GG+ +KSCPEQI CG+M  +S ++HS PAD   T NN+QRKYF SHW+++DV+
Sbjct: 121  NLHPLDAGGKTIKSCPEQIVCGRMPGISTNQHSSPAD--VTENNSQRKYFASHWSVEDVD 180

Query: 181  ERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILINGIASQNRALEGDIVAIMVDPFTS 240
            E LQKG IF+A FRVNAHNRLEAYCKIDGLPVD+LINGIASQNRA+EGDIVAIMVDP TS
Sbjct: 181  EGLQKGDIFRAFFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIMVDPLTS 240

Query: 241  WTMMKGTCEAHNNKHSMEDANLPAEETEKNSHNCKDKNKVNSDVKSDSYRSSSSSDKRCC 300
            WT MKGT E HN+ HSMEDANLPAE TE +  NCK KNKV++ VKSDS+RSSSS DKRCC
Sbjct: 241  WTRMKGTSETHNSTHSMEDANLPAEATENDGRNCKGKNKVDASVKSDSFRSSSSPDKRCC 300

Query: 301  SEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHYSSIQDDVSMAIGRICTLINLYPSK 360
            SE+K+LDGTACDDLL   E  D Y    V   +AHYSS QDDVS AI RIC +I+ YP K
Sbjct: 301  SEDKILDGTACDDLLLKNEQRDVYQSLVVDLPEAHYSSNQDDVSKAIQRICAVISSYPGK 360

Query: 361  RPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYTKEMKTKSCLSSSHDSGYVQLMPND 420
            RPTGRVVAI+EKSR RE+IVGHLNVKKFLSF EIY KEM TKSCLS SH+ GYVQLMPND
Sbjct: 361  RPTGRVVAILEKSRQRESIVGHLNVKKFLSFQEIYMKEMNTKSCLSPSHNCGYVQLMPND 420

Query: 421  ARFPIMIVLARDLPDCIKKRLENGDVTVESELVAARIDEWVEESSAPRAQVLHVLGQGSK 480
            ARFPIM+VLA DLPD IKKRL+NGDVTVE+ELVA +I EWV+ESSAP+A VLHVLG+GS+
Sbjct: 421  ARFPIMVVLAGDLPDSIKKRLDNGDVTVENELVAVKIHEWVKESSAPQAHVLHVLGRGSE 480

Query: 481  IESHIDAILFENAIRKCEFSQDSLSCLPRTPWKIPHEELQCRRDLRNLCIFTIDPSSVLD 540
            + SHIDAILFENAI  CEFS DSL+CLP TPWKIPHEELQCRRDLRNLCIFTIDPSS  D
Sbjct: 481  VASHIDAILFENAIHSCEFSNDSLACLPHTPWKIPHEELQCRRDLRNLCIFTIDPSSASD 540

Query: 541  LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQHKIPMLPPLLS 600
            LDDALSVQKLANGIFRVG+HIADVSHFVLPDTALDKEAQIRSTSVYLLQ KIPMLPPLLS
Sbjct: 541  LDDALSVQKLANGIFRVGVHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS 600

Query: 601  ENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSCCKLSYEHAQDIIDGLIDSDS 660
            EN+ SL+PGVDRLAFSLFLD+D+CG VKD WI RTVICSCCKLSYEHAQDIIDGLIDSDS
Sbjct: 601  ENVGSLSPGVDRLAFSLFLDIDNCGDVKDRWIGRTVICSCCKLSYEHAQDIIDGLIDSDS 660

Query: 661  SKILG-NCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRDGALRLENSKIVYLYDEYGI 720
            SK LG N PQLH QF WLDVISSVKILHEISKTLK+KRFRDGALRLENSKIVYLYDEYGI
Sbjct: 661  SKNLGNNYPQLHGQFEWLDVISSVKILHEISKTLKKKRFRDGALRLENSKIVYLYDEYGI 720

Query: 721  PYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTYPNSALLRRQPEPILRKLREFESFCS 780
            PYDS FYEQKDSNFLVEEFMLLAN TVAEVISRT+P+SALLRR PEPILRKLREFESFCS
Sbjct: 721  PYDSAFYEQKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPILRKLREFESFCS 780

Query: 781  KHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYATRPMQLATYFCNGELKDGENG 840
            KHG ELDTSSSV+FQQSLEQIRIKLHDDP LF+I+ SYATRPMQLATYFC+GELKDGE G
Sbjct: 781  KHGFELDTSSSVQFQQSLEQIRIKLHDDPLLFDILTSYATRPMQLATYFCSGELKDGEKG 840

Query: 841  SHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEAEELYLKHQGIIPKVNSDEQMRCFTG 900
            SHYALA PLYTHFTSPLRRYPDI+VHRTLAAA+EAE+LYLKH+GI  KVNSDEQ+RCFTG
Sbjct: 841  SHYALAVPLYTHFTSPLRRYPDIIVHRTLAAAIEAEKLYLKHRGITQKVNSDEQIRCFTG 900

Query: 901  IYFDKDAADSSEGREALSSAASRHGLPCSKLLSDVALHCNNRKLASKHVADACNKLYMWA 960
            +YFDKDAADS EG+EALSSAA RHG+PC+KLL+DVALHCNNRKLASKHVAD C+KLYMWA
Sbjct: 901  MYFDKDAADSLEGKEALSSAALRHGVPCAKLLADVALHCNNRKLASKHVADGCDKLYMWA 960

Query: 961  LLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLEVEWLDATSTLVLSFFG 1020
            LLK+KKVLFSDARVLGLGPRFMS+YIQKLAIERRIYYDE EGL VEWL+ TSTLVLSFFG
Sbjct: 961  LLKKKKVLFSDARVLGLGPRFMSLYIQKLAIERRIYYDETEGLAVEWLETTSTLVLSFFG 1020

Query: 1021 TRRPHRNRGSSIKWKALEDVALVISPCDLNVKQRTLGGMSLSESGGAGTGGASVEQEPNL 1080
            TRR HR+RG SIKWKALEDVALV+SPCD NVKQR LG  S SE GG GTGGA VEQE NL
Sbjct: 1021 TRRSHRSRG-SIKWKALEDVALVVSPCDQNVKQRALGA-SPSELGGTGTGGAVVEQESNL 1080

Query: 1081 KSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYTSSYLK 1131
            KSHVSDTGIDPAVFPLTVRLLST+PVALHAVGGDDGPIDIGVRLY SSYLK
Sbjct: 1081 KSHVSDTGIDPAVFPLTVRLLSTLPVALHAVGGDDGPIDIGVRLYMSSYLK 1127

BLAST of Sed0025890.3 vs. ExPASy TrEMBL
Match: A0A6J1FJM2 (DIS3-like exonuclease 2 OS=Cucurbita moschata OX=3662 GN=LOC111444646 PE=3 SV=1)

HSP 1 Score: 1840.5 bits (4766), Expect = 0.0e+00
Identity = 926/1131 (81.87%), Postives = 1013/1131 (89.57%), Query Frame = 0

Query: 1    MRGAVEQSTPDRNDDGEKEKKKKRRYSRRSKHSASIATPVSCSSVNEISGEASECMENGR 60
            MRGAVEQSTP+R DDG+KEKKKKRR +RRSK +ASI+T VSCSSVN + GEASEC ENG+
Sbjct: 1    MRGAVEQSTPERYDDGDKEKKKKRRSNRRSKQNASISTSVSCSSVNGMPGEASECRENGK 60

Query: 61   IDANLTAPSNSSSPTQQACQSIHPSEPGLTGRNKISFGSLPPLHISKNAELSELQYSINQ 120
            I+ANLTAPSN SS TQQA +S HP E G+T RNKI+F SLPPLHIS+ AELSE Q  IN+
Sbjct: 61   INANLTAPSNHSSLTQQAHESNHPIEHGVTRRNKIAFSSLPPLHISEQAELSESQNLINE 120

Query: 121  DLYSLNTGGRIVKSCPEQIACGKMLQLSMSEHSPPADANATVNNTQRKYFTSHWAMDDVN 180
            +L+ L+ GG+ +KSCPEQI CG+M  +S+++HSPPAD   T NNTQRKYF SHW+++DV+
Sbjct: 121  NLHPLDAGGKTIKSCPEQIVCGRMPGISINQHSPPAD--VTENNTQRKYFASHWSVEDVD 180

Query: 181  ERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILINGIASQNRALEGDIVAIMVDPFTS 240
            E LQKG IF+A FRVN+HNRLEAYCKIDGLPVD+LINGIASQNRA+EGDIVAIMVDP TS
Sbjct: 181  EGLQKGDIFRAFFRVNSHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIMVDPLTS 240

Query: 241  WTMMKGTCEAHNNKHSMEDANLPAEETEKNSHNCKDKNKVNSDVKSDSYRSSSSSDKRCC 300
            WT MKGT E HN+ HSMEDANLPAE TE +  NCK KNK+++ VKSDS+RSSSS DKRCC
Sbjct: 241  WTRMKGTSETHNSTHSMEDANLPAEATENDGRNCKGKNKLDASVKSDSFRSSSSPDKRCC 300

Query: 301  SEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHYSSIQDDVSMAIGRICTLINLYPSK 360
            SE+K+LDGTACDDLL   E  D Y  S V P +AHYS  QDDVS AI RIC +I+ YP K
Sbjct: 301  SEDKILDGTACDDLLLKNEQRDVYQSSVVDPPEAHYSRNQDDVSKAIQRICAVISSYPGK 360

Query: 361  RPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYTKEMKTKSCLSSSHDSGYVQLMPND 420
            RPTGRVVAI+EKSR RE+IVGHLNVKKFLSF EIY KEM TKSCLS SH+ GYVQLMPND
Sbjct: 361  RPTGRVVAILEKSRQRESIVGHLNVKKFLSFQEIYMKEMNTKSCLSPSHNCGYVQLMPND 420

Query: 421  ARFPIMIVLARDLPDCIKKRLENGDVTVESELVAARIDEWVEESSAPRAQVLHVLGQGSK 480
            ARFPIM+VLA DLPD IKKRL+NGDVTVE+ELVA +I EWV+ESSAP+A VLHVLG+GS+
Sbjct: 421  ARFPIMMVLAGDLPDSIKKRLDNGDVTVENELVAVKIHEWVKESSAPQALVLHVLGRGSE 480

Query: 481  IESHIDAILFENAIRKCEFSQDSLSCLPRTPWKIPHEELQCRRDLRNLCIFTIDPSSVLD 540
            + SHIDAILFENAI  CEFS DSL+CLP TPWKIPHEELQCRRDLRNLCIFTIDPSS  D
Sbjct: 481  VASHIDAILFENAIHSCEFSNDSLACLPHTPWKIPHEELQCRRDLRNLCIFTIDPSSASD 540

Query: 541  LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQHKIPMLPPLLS 600
            LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQ KIPMLPPLLS
Sbjct: 541  LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS 600

Query: 601  ENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSCCKLSYEHAQDIIDGLIDSDS 660
            +N+ SL+PGVDRLAFSLFLD+D+ G VKD WI RTVICSCCKLSYEHAQDIIDGLIDSD+
Sbjct: 601  KNVGSLSPGVDRLAFSLFLDIDNSGDVKDRWIGRTVICSCCKLSYEHAQDIIDGLIDSDN 660

Query: 661  SKILG-NCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRDGALRLENSKIVYLYDEYGI 720
             K LG N PQLH QF+W DVISSVK+LHEISKTLK+KRFRDGALRLENSKIVYLYDEYGI
Sbjct: 661  LKNLGNNYPQLHGQFAWPDVISSVKLLHEISKTLKKKRFRDGALRLENSKIVYLYDEYGI 720

Query: 721  PYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTYPNSALLRRQPEPILRKLREFESFCS 780
            PYDSTFYEQKDSNFLVEEFMLLAN TVAEVISRT+P+SALLRR PEPILRKLREFESFCS
Sbjct: 721  PYDSTFYEQKDSNFLVEEFMLLANTTVAEVISRTFPDSALLRRHPEPILRKLREFESFCS 780

Query: 781  KHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYATRPMQLATYFCNGELKDGENG 840
            KHG ELDTSSSV+FQQSLEQIRIKLHDDP LF+I+ SYATRPMQLATYFC+GELKDGE G
Sbjct: 781  KHGFELDTSSSVQFQQSLEQIRIKLHDDPLLFDILTSYATRPMQLATYFCSGELKDGEKG 840

Query: 841  SHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEAEELYLKHQGIIPKVNSDEQMRCFTG 900
            SHYALA PLYTHFTSPLRRYPDIVVHRTLAAA+EAE+LYLKH+GII KVNSDEQ+RCFTG
Sbjct: 841  SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKLYLKHRGIIQKVNSDEQIRCFTG 900

Query: 901  IYFDKDAADSSEGREALSSAASRHGLPCSKLLSDVALHCNNRKLASKHVADACNKLYMWA 960
            +YFDKDAADS EGREALSSAA RHG+PC+KLL+DVALHCN+RKLASKHVAD C+KLYMWA
Sbjct: 901  MYFDKDAADSLEGREALSSAALRHGVPCAKLLADVALHCNDRKLASKHVADGCDKLYMWA 960

Query: 961  LLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLEVEWLDATSTLVLSFFG 1020
            +LK+KKVLFSDARVLGLGPRFMS+YIQKLAIERRIYYDE EGL VEWL+ TSTLVLSFFG
Sbjct: 961  VLKKKKVLFSDARVLGLGPRFMSLYIQKLAIERRIYYDETEGLAVEWLETTSTLVLSFFG 1020

Query: 1021 TRRPHRNRGSSIKWKALEDVALVISPCDLNVKQRTLGGMSLSESGGAGTGGASVEQEPNL 1080
            TRR HR+RG SIKWKALEDVALV+SPCD NVKQR L G+S SE GG GTGGA VEQE NL
Sbjct: 1021 TRRSHRSRG-SIKWKALEDVALVVSPCDHNVKQRAL-GVSPSELGGTGTGGAVVEQESNL 1080

Query: 1081 KSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYTSSYLK 1131
            KSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLY SSYLK
Sbjct: 1081 KSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYMSSYLK 1127

BLAST of Sed0025890.3 vs. ExPASy TrEMBL
Match: A0A0A0KC80 (DIS3-like exonuclease 2 OS=Cucumis sativus OX=3659 GN=Csa_6G040560 PE=3 SV=1)

HSP 1 Score: 1805.4 bits (4675), Expect = 0.0e+00
Identity = 909/1132 (80.30%), Postives = 1005/1132 (88.78%), Query Frame = 0

Query: 1    MRGAVEQSTPDRNDDGEKEKKKKRRYSRRSKHSASIATPVSCSSVNEISGEASECMENGR 60
            MR AVEQSTP+RN DG+KEKKKKRR +RRSKH+ S+ T  S +SVN I GEASECMENGR
Sbjct: 1    MRAAVEQSTPERNGDGDKEKKKKRRSNRRSKHNPSLTTSASYTSVNGILGEASECMENGR 60

Query: 61   IDANLTAPSNSSSPTQQACQSIHPSEPGLTGRNKISFGSLPPLHISKNAELSELQYSINQ 120
            IDANLT+PSN SS TQQ   S    E GLT  +KI F SLPPLHI++ AELS     +NQ
Sbjct: 61   IDANLTSPSNYSSLTQQENHSNQQIEHGLTRGDKIGFSSLPPLHINEQAELSASHNLMNQ 120

Query: 121  DLYSLNTGGRIVKSCPEQIACGKMLQLSMSEHSPPADANATVNNTQRKYFTSHWAMDDVN 180
            + +S + GGR+ KSCPEQIA G+   +S+++HSPPAD   T NNTQRKYF SHW++DDVN
Sbjct: 121  NHHSSDAGGRVTKSCPEQIASGRYSGISLNQHSPPAD--VTDNNTQRKYFPSHWSVDDVN 180

Query: 181  ERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILINGIASQNRALEGDIVAIMVDPFTS 240
            E LQKG IFKALFRVNAHNRLEAYCKIDGLP+D+LINGIASQNRA+EGDIVAI +DPFTS
Sbjct: 181  EGLQKGGIFKALFRVNAHNRLEAYCKIDGLPIDVLINGIASQNRAVEGDIVAIKLDPFTS 240

Query: 241  WTMMKGTCEAHNNKHSMEDANLPAEETEKNSHNCKDKNKVNSDVKSDSYRSSSSSDKRCC 300
            WT MKGT EAHNN HSMEDANLPAE TEKN HNCK KNKV++DVKSDS+RS+S  DKRCC
Sbjct: 241  WTKMKGTSEAHNNMHSMEDANLPAELTEKNDHNCKGKNKVDADVKSDSFRSTSLPDKRCC 300

Query: 301  SEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHYSSIQDDVSMAIGRICTLINLYPSK 360
            SE+KVLDG ACD LLSNYE CD    S V PSQAH+SS QDDVS AIGRIC LINLYP+K
Sbjct: 301  SEDKVLDGVACDVLLSNYEQCDINELSVVNPSQAHHSSNQDDVSKAIGRICALINLYPAK 360

Query: 361  RPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYTKEMKTKSCLSSSHDSGYVQLMPND 420
            RPTGRVV I+EKSR RE +VGHLNVKKFLSF E Y KE  TKSCLS S + GYVQLMPND
Sbjct: 361  RPTGRVVTILEKSRLRENVVGHLNVKKFLSFQEFYVKE-STKSCLSPSQNCGYVQLMPND 420

Query: 421  ARFPIMIVLARDLPDCIKKRLENGDVTVESELVAARIDEWVEESSAPRAQVLHVLGQGSK 480
            ARFPIM+VLA DLP+CIKKRL+NGDVTVE+ELVAARI EWV+ESS+PRA VLHVLG+G++
Sbjct: 421  ARFPIMMVLAGDLPNCIKKRLDNGDVTVENELVAARIYEWVKESSSPRAHVLHVLGRGNE 480

Query: 481  IESHIDAILFENAIRKCEFSQDSLSCLPRTPWKIPHEELQCRRDLRNLCIFTIDPSSVLD 540
            +ESHIDAILFENAIR CEFSQDSLSC+P+TPWKIP EELQCRRD+RNLCIFTIDPSS  D
Sbjct: 481  VESHIDAILFENAIRTCEFSQDSLSCVPQTPWKIPPEELQCRRDIRNLCIFTIDPSSASD 540

Query: 541  LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQHKIPMLPPLLS 600
            LDDALSVQ+LANGIFRVGIHIADVS+FVLPDTALDKEAQIRSTSVYLLQ KIPMLPPLLS
Sbjct: 541  LDDALSVQRLANGIFRVGIHIADVSYFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS 600

Query: 601  ENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSCCKLSYEHAQDIIDGLIDSDS 660
            E++ SLNPGVDRLAFSLFLD++ CG VKD+WI+RTVIC CCKLSYEHAQDIIDGLIDSDS
Sbjct: 601  ESIGSLNPGVDRLAFSLFLDINSCGDVKDFWIERTVICCCCKLSYEHAQDIIDGLIDSDS 660

Query: 661  SKILG-NCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRDGALRLENSKIVYLYDEYGI 720
            S++ G NCPQLH QF+W DVISSVK+LHEISKT+KEKRFR+GALRLENSK++YLYDEYGI
Sbjct: 661  SELFGNNCPQLHGQFTWHDVISSVKLLHEISKTVKEKRFRNGALRLENSKLIYLYDEYGI 720

Query: 721  PYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTYPNSALLRRQPEPILRKLREFESFCS 780
            PYDS FYEQKDSNFLVEEFMLLANRTVAEVISRT+P+SALLRR PEP+LRKLREFE+FCS
Sbjct: 721  PYDSMFYEQKDSNFLVEEFMLLANRTVAEVISRTFPDSALLRRHPEPMLRKLREFETFCS 780

Query: 781  KHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYATRPMQLATYFCNGELKDGENG 840
            KHG ELDTSSSV FQQSLEQIRI+L DDP LF+I+ISYATRPMQLATYFC+GELKDGE  
Sbjct: 781  KHGFELDTSSSVHFQQSLEQIRIELQDDPLLFDILISYATRPMQLATYFCSGELKDGETR 840

Query: 841  SHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEAEELYLKHQGIIPKVNSDEQMRCFTG 900
            SHYALA PLYTHFTSPLRRYPDIVVHRTLAAA+EAE++YLKH+G+I KVNS+E+ RCFTG
Sbjct: 841  SHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKMYLKHKGVIQKVNSNEETRCFTG 900

Query: 901  IYFDKDAADSSEGREALSSAASRHGLPCSKLLSDVALHCNNRKLASKHVADACNKLYMWA 960
            IYFDKDAADS EGREALSSAA +HG+PCSKLL DVALHCN+RKLASKHVAD   KLYMWA
Sbjct: 901  IYFDKDAADSLEGREALSSAALKHGVPCSKLLLDVALHCNDRKLASKHVADGIEKLYMWA 960

Query: 961  LLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLEVEWLDATSTLVLSFFG 1020
            LLK+KK+LFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGL VEWL+ TSTLVL FF 
Sbjct: 961  LLKKKKILFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLAVEWLETTSTLVLRFFC 1020

Query: 1021 TRRPHRNRGSSIKWKALEDVALVISPCDLNVKQRTLGGMSLSESGGAGTGG-ASVEQEPN 1080
            +RR HR+RG S+KWKALEDVALVISPCD NVK+RTLG   +S +GGA  GG A VEQ+ N
Sbjct: 1021 SRRSHRSRG-SVKWKALEDVALVISPCDQNVKERTLG---VSSNGGASKGGSAVVEQDSN 1080

Query: 1081 LKSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYTSSYLK 1131
            LKSHVSDTG+DPA+FPLTVRLLSTIPVALHAVGGDDGPIDIGVRLY SSYL+
Sbjct: 1081 LKSHVSDTGVDPAIFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYMSSYLR 1125

BLAST of Sed0025890.3 vs. ExPASy TrEMBL
Match: A0A1S3BDZ0 (DIS3-like exonuclease 2 OS=Cucumis melo OX=3656 GN=LOC103488626 PE=3 SV=1)

HSP 1 Score: 1797.3 bits (4654), Expect = 0.0e+00
Identity = 912/1132 (80.57%), Postives = 998/1132 (88.16%), Query Frame = 0

Query: 1    MRGAVEQSTPDRNDDGEKEKKKKRRYSRRSKHSASIATPVSCSSVNEISGEASECMENGR 60
            MR A EQSTP+RN D +KEKKKKRR +RRSKH+ S+ T  S +SVN I GEASECMENGR
Sbjct: 1    MRAAFEQSTPERNGDCDKEKKKKRRSNRRSKHNPSLTTSASYTSVNGILGEASECMENGR 60

Query: 61   IDANLTAPSNSSSPTQQACQSIHPSEPGLTGRNKISFGSLPPLHISKNAELSELQYSINQ 120
            IDANLT+PSN SS TQQ   S HP E GLTG NKI+F SLP LHI+  AELS  Q  INQ
Sbjct: 61   IDANLTSPSNYSSLTQQENHSNHPIEHGLTGGNKIAFSSLPSLHINDQAELSASQNLINQ 120

Query: 121  DLYSLNTGGRIVKSCPEQIACGKMLQLSMSEHSPPADANATVNNTQRKYFTSHWAMDDVN 180
            + +S + GGRI+KSCPEQIA G+   +S ++ SPPAD   T NNTQRKYF SHW++DDVN
Sbjct: 121  NHHSSDAGGRIIKSCPEQIASGRNSGISSNQLSPPAD--LTENNTQRKYFPSHWSIDDVN 180

Query: 181  ERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILINGIASQNRALEGDIVAIMVDPFTS 240
            E LQKG IFKALFRVNAHNRLEAYCKIDGLPVD+LINGIASQNRA+EGDIVAI VDPFT 
Sbjct: 181  EGLQKGDIFKALFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIKVDPFTV 240

Query: 241  WTMMKGTCEAHNNKHSMEDANLPAEETEKNSHNCKDKNKVNSDVKSDSYRSSSSSDKRCC 300
            WT MKGT EAH+N  SM+DANLPAE TEKNSHNCK KNK ++D KSDS+RSSS  DKRCC
Sbjct: 241  WTKMKGTSEAHDNIKSMDDANLPAEPTEKNSHNCKGKNKFDADGKSDSFRSSSLPDKRCC 300

Query: 301  SEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHYSSIQDDVSMAIGRICTLINLYPSK 360
            SE+KVLDG +CDDLLSNYE CD    S V PSQAH+SS Q DVS  IGRIC LINLYP+K
Sbjct: 301  SEDKVLDGISCDDLLSNYEQCDINQLSVVDPSQAHHSSNQYDVSKIIGRICALINLYPAK 360

Query: 361  RPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYTKEMKTKSCLSSSHDSGYVQLMPND 420
            RPTGRVV I+EKSR R+ +VGHLNVKKFLSF E Y KE  TKSCLS S + GYVQLMPND
Sbjct: 361  RPTGRVVTILEKSRLRDNVVGHLNVKKFLSFQEFYVKE-NTKSCLSPSQNGGYVQLMPND 420

Query: 421  ARFPIMIVLARDLPDCIKKRLENGDVTVESELVAARIDEWVEESSAPRAQVLHVLGQGSK 480
            ARFPIM+VLA DLPDCIKKRL+NGDVTVE+ELVAARI +WV+ESS+PRA VLHVLG+GS+
Sbjct: 421  ARFPIMMVLAGDLPDCIKKRLDNGDVTVENELVAARIYDWVKESSSPRAHVLHVLGRGSE 480

Query: 481  IESHIDAILFENAIRKCEFSQDSLSCLPRTPWKIPHEELQCRRDLRNLCIFTIDPSSVLD 540
            +ESHIDAILFENAIR CEFS DSLSC+P TPWKIPHEEL+CRRD+RNLCIFTIDPSS  D
Sbjct: 481  VESHIDAILFENAIRTCEFSHDSLSCIPHTPWKIPHEELRCRRDIRNLCIFTIDPSSASD 540

Query: 541  LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQHKIPMLPPLLS 600
            LDDALSVQKLAN IFRVGIHIADVS+FVLPDTALDKEAQIRSTSVYLLQ KIPMLPPLLS
Sbjct: 541  LDDALSVQKLANDIFRVGIHIADVSYFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS 600

Query: 601  ENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSCCKLSYEHAQDIIDGLIDSDS 660
            EN+ SLNPGVDRLAFSLFLD++ CG VKDYWI+RTVIC CCKLSYE+AQDIIDGLIDSDS
Sbjct: 601  ENIGSLNPGVDRLAFSLFLDINGCGDVKDYWIERTVICCCCKLSYEYAQDIIDGLIDSDS 660

Query: 661  SKILG-NCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRDGALRLENSKIVYLYDEYGI 720
             +I G NCPQLH QF+W DVISSVK+LHEISKTLKEKRFRDGALRLENSK++YLYDEYGI
Sbjct: 661  PEIFGNNCPQLHGQFTWHDVISSVKLLHEISKTLKEKRFRDGALRLENSKLIYLYDEYGI 720

Query: 721  PYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTYPNSALLRRQPEPILRKLREFESFCS 780
            PYDS FYEQKDSNFLVEEFMLLANRTVAEVISRT+P+SALLRR PEP+LRKLREFESFCS
Sbjct: 721  PYDSMFYEQKDSNFLVEEFMLLANRTVAEVISRTFPDSALLRRHPEPMLRKLREFESFCS 780

Query: 781  KHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYATRPMQLATYFCNGELKDGENG 840
            KHG ELDTSSSV FQQSLEQIR KLHDDP LF+I+ISYATRPMQLATYFC+GELKDGE  
Sbjct: 781  KHGFELDTSSSVHFQQSLEQIRTKLHDDPLLFDILISYATRPMQLATYFCSGELKDGEKR 840

Query: 841  SHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEAEELYLKHQGIIPKVNSDEQMRCFTG 900
            +HYALA PLYTHFTSPLRRYPDIVVHRTLAAA+EAE++YLKHQGII KVNSD++MRCFTG
Sbjct: 841  NHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKVYLKHQGIIQKVNSDKEMRCFTG 900

Query: 901  IYFDKDAADSSEGREALSSAASRHGLPCSKLLSDVALHCNNRKLASKHVADACNKLYMWA 960
            IYFDKDAADS EGREALS AA +HG+PCSKLLSDVALHCN+RKLASKH+AD C KLYMWA
Sbjct: 901  IYFDKDAADSLEGREALSFAALKHGVPCSKLLSDVALHCNDRKLASKHIADGCEKLYMWA 960

Query: 961  LLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLEVEWLDATSTLVLSFFG 1020
            LLK+K++LFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGL VEWLD TSTLVLSF  
Sbjct: 961  LLKKKRILFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLAVEWLDTTSTLVLSFC- 1020

Query: 1021 TRRPHRNRGSSIKWKALEDVALVISPCDLNVKQRTLGGMSLSESGGAGTGG-ASVEQEPN 1080
            +RR HR+RG S+KWKALEDVALVISPCD NV +RTLG   +  +GGA  GG A+VEQ+ N
Sbjct: 1021 SRRSHRSRG-SVKWKALEDVALVISPCDQNVNKRTLG---VCPNGGASKGGSAAVEQDSN 1080

Query: 1081 LKSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYTSSYLK 1131
            LKSHVSD G+DPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLY SSYL+
Sbjct: 1081 LKSHVSDIGVDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYMSSYLR 1124

BLAST of Sed0025890.3 vs. ExPASy TrEMBL
Match: A0A5D3CLM1 (DIS3-like exonuclease 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1827G00340 PE=3 SV=1)

HSP 1 Score: 1786.9 bits (4627), Expect = 0.0e+00
Identity = 905/1122 (80.66%), Postives = 990/1122 (88.24%), Query Frame = 0

Query: 1    MRGAVEQSTPDRNDDGEKEKKKKRRYSRRSKHSASIATPVSCSSVNEISGEASECMENGR 60
            MR A EQSTP+RN D +KEKKKKRR +RRSKH+ S+ T  S +SVN I GEASECMENGR
Sbjct: 1    MRAAFEQSTPERNGDCDKEKKKKRRSNRRSKHNPSLTTSASYTSVNGILGEASECMENGR 60

Query: 61   IDANLTAPSNSSSPTQQACQSIHPSEPGLTGRNKISFGSLPPLHISKNAELSELQYSINQ 120
            IDANLT+PSN SS TQQ   S HP E GLTG NKI+F SLP LHI+  AELS  Q  INQ
Sbjct: 61   IDANLTSPSNYSSLTQQENHSNHPIEHGLTGGNKIAFSSLPSLHINDQAELSASQNLINQ 120

Query: 121  DLYSLNTGGRIVKSCPEQIACGKMLQLSMSEHSPPADANATVNNTQRKYFTSHWAMDDVN 180
            + +S + GGRI+KSCPEQIA G+   +S ++ SPPAD   T NNTQRKYF SHW++DDVN
Sbjct: 121  NHHSSDAGGRIIKSCPEQIASGRNSGISSNQLSPPAD--LTENNTQRKYFPSHWSIDDVN 180

Query: 181  ERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILINGIASQNRALEGDIVAIMVDPFTS 240
            E LQKG IFKALFRVNAHNRLEAYCKIDGLPVD+LINGIASQNRA+EGDIVAI VDPFT 
Sbjct: 181  EGLQKGDIFKALFRVNAHNRLEAYCKIDGLPVDVLINGIASQNRAVEGDIVAIKVDPFTV 240

Query: 241  WTMMKGTCEAHNNKHSMEDANLPAEETEKNSHNCKDKNKVNSDVKSDSYRSSSSSDKRCC 300
            WT MKGT EAH+N  SM+DANLPAE TEKNSHNCK KNK ++D KSDS+RSSS  DKRCC
Sbjct: 241  WTKMKGTSEAHDNIKSMDDANLPAEPTEKNSHNCKGKNKFDADGKSDSFRSSSLPDKRCC 300

Query: 301  SEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHYSSIQDDVSMAIGRICTLINLYPSK 360
            SE+KVLDG +CDDLLSNYE CD    S V PSQAH+SS Q DVS  IGRIC LINLYP+K
Sbjct: 301  SEDKVLDGISCDDLLSNYEQCDINQLSVVDPSQAHHSSNQYDVSKIIGRICALINLYPAK 360

Query: 361  RPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYTKEMKTKSCLSSSHDSGYVQLMPND 420
            RPTGRVV I+EKSR R+ +VGHLNVKKFLSF E Y KE  TKSCLS S + GYVQLMPND
Sbjct: 361  RPTGRVVTILEKSRLRDNVVGHLNVKKFLSFQEFYVKE-NTKSCLSPSQNGGYVQLMPND 420

Query: 421  ARFPIMIVLARDLPDCIKKRLENGDVTVESELVAARIDEWVEESSAPRAQVLHVLGQGSK 480
            ARFPIM+VLA DLPDCIKKRL+NGDVTVE+ELVAARI +WV+ESS+PRA VLHVLG+GS+
Sbjct: 421  ARFPIMMVLAGDLPDCIKKRLDNGDVTVENELVAARIYDWVKESSSPRAHVLHVLGRGSE 480

Query: 481  IESHIDAILFENAIRKCEFSQDSLSCLPRTPWKIPHEELQCRRDLRNLCIFTIDPSSVLD 540
            +ESHIDAILFENAIR CEFS DSLSC+P TPWKIPHEEL+CRRD+RNLCIFTIDPSS  D
Sbjct: 481  VESHIDAILFENAIRTCEFSHDSLSCIPHTPWKIPHEELRCRRDIRNLCIFTIDPSSASD 540

Query: 541  LDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQHKIPMLPPLLS 600
            LDDALSVQKLAN IFRVGIHIADVS+FVLPDTALDKEAQIRSTSVYLLQ KIPMLPPLLS
Sbjct: 541  LDDALSVQKLANDIFRVGIHIADVSYFVLPDTALDKEAQIRSTSVYLLQRKIPMLPPLLS 600

Query: 601  ENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSCCKLSYEHAQDIIDGLIDSDS 660
            EN+ SLNPGVDRLAFSLFLD++ CG VKDYWI+RTVIC CCKLSYE+AQDIIDGLIDSDS
Sbjct: 601  ENIGSLNPGVDRLAFSLFLDINGCGDVKDYWIERTVICCCCKLSYEYAQDIIDGLIDSDS 660

Query: 661  SKILG-NCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRDGALRLENSKIVYLYDEYGI 720
             +I G NCPQLH QF+W DVISSVK+LHEISKTLKEKRFRDGALRLENSK++YLYDEYGI
Sbjct: 661  PEIFGNNCPQLHGQFTWHDVISSVKLLHEISKTLKEKRFRDGALRLENSKLIYLYDEYGI 720

Query: 721  PYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTYPNSALLRRQPEPILRKLREFESFCS 780
            PYDS FYEQKDSNFLVEEFMLLANRTVAEVISRT+P+SALLRR PEP+LRKLREFESFCS
Sbjct: 721  PYDSMFYEQKDSNFLVEEFMLLANRTVAEVISRTFPDSALLRRHPEPMLRKLREFESFCS 780

Query: 781  KHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYATRPMQLATYFCNGELKDGENG 840
            KHG ELDTSSSV FQQSLEQIR KLHDDP LF+I+ISYATRPMQLATYFC+GELKDGE  
Sbjct: 781  KHGFELDTSSSVHFQQSLEQIRTKLHDDPLLFDILISYATRPMQLATYFCSGELKDGEKR 840

Query: 841  SHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEAEELYLKHQGIIPKVNSDEQMRCFTG 900
            +HYALA PLYTHFTSPLRRYPDIVVHRTLAAA+EAE++YLKHQGII KVNSD++MRCFTG
Sbjct: 841  NHYALAVPLYTHFTSPLRRYPDIVVHRTLAAAIEAEKVYLKHQGIIQKVNSDKEMRCFTG 900

Query: 901  IYFDKDAADSSEGREALSSAASRHGLPCSKLLSDVALHCNNRKLASKHVADACNKLYMWA 960
            IYFDKDAADS EGREALS AA +HG+PCSKLLSDVALHCN+RKLASKH+AD C KLYMWA
Sbjct: 901  IYFDKDAADSLEGREALSFAALKHGVPCSKLLSDVALHCNDRKLASKHIADGCEKLYMWA 960

Query: 961  LLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLEVEWLDATSTLVLSFFG 1020
            LLK+K++LFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGL VEWLD TSTLVLSFF 
Sbjct: 961  LLKKKRILFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLAVEWLDTTSTLVLSFFC 1020

Query: 1021 TRRPHRNRGSSIKWKALEDVALVISPCDLNVKQRTLGGMSLSESGGAGTGG-ASVEQEPN 1080
            +RR HR+RG S+KWKALEDVALVISPCD NV +RTLG   +  +GGA  GG A+VEQ+ N
Sbjct: 1021 SRRSHRSRG-SVKWKALEDVALVISPCDQNVNKRTLG---VCPNGGASKGGSAAVEQDSN 1080

Query: 1081 LKSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIG 1121
            LKSHVSD G+DPAVFPLTVRLLSTIPVALHAVGGDDGPIDIG
Sbjct: 1081 LKSHVSDIGVDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIG 1115

BLAST of Sed0025890.3 vs. TAIR 10
Match: AT1G77680.1 (Ribonuclease II/R family protein )

HSP 1 Score: 959.1 bits (2478), Expect = 3.1e-279
Identity = 552/1134 (48.68%), Postives = 725/1134 (63.93%), Query Frame = 0

Query: 1    MRGAVEQSTPDRNDDGEKEKKKK-RRYSRRSKHSASIATPVSCSSVNEISGEASECMENG 60
            M+ A  + + +R ++G K+K+ + ++ +RRSK S   + P+  + V E          +G
Sbjct: 1    MKSASSEQSVERIENGHKKKRNRPQKQNRRSKQS---SVPIEDAHVEE--------SLDG 60

Query: 61   RIDANLTAPSNSSSPTQQACQSIHPSEPGLTGRNKISFGSLPPLHISKNAELSELQYSIN 120
            R  +   A  ++SS  QQ   +    E      + ++F S+PP+     AE    + S +
Sbjct: 61   RDSSRSKAKDSTSSSKQQRPNT---DELEAMRASNVAFNSMPPM----RAESGYPRRSAS 120

Query: 121  QDLYSLNTGGRIV-KSCPEQIACGKMLQLSMSEHSPPADA---NATVNNTQRKYFTSHWA 180
              L S     +++ KSCP+  AC         E SP  +         ++QRK F+SHW+
Sbjct: 121  PLLSSPEVSKQLLSKSCPDPRAC---------EQSPGMNGELFQQIEGSSQRKIFSSHWS 180

Query: 181  MDDVNERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILINGIASQNRALEGDIVAIMV 240
            +D V E L+KG  FKALFRVNAHNR EAYCKIDG+P DILING   Q+RA+EGD V I +
Sbjct: 181  LDAVTEALEKGEAFKALFRVNAHNRNEAYCKIDGVPTDILINGNVCQSRAVEGDTVVIKL 240

Query: 241  DPFTSWTMMKGTCEAHNNKHSMEDANLPAEETEKNSHNCKDKNKVNSDVKSDSYRSSSSS 300
            DP + W  MKG       K   E  N P E+ +K +      + V       S   SS  
Sbjct: 241  DPLSLWPKMKGFVTESAAK--PEGTNSPPEKDDKKARQKNGIDVVEGFEDGFSKNKSSVI 300

Query: 301  DKRCCSEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHYSSIQDDVSMAIGRICTLIN 360
             K   +        + D  L +  +C+                 Q     A+ ++C +++
Sbjct: 301  GKGAKNGVTPSSPPSLDSCLGS--FCE-----------------QKGNCSAVDKLCGILS 360

Query: 361  LYPSKRPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYTKEMKTKSCLSSSHDSGYVQ 420
             +P KRPTG+VVA++EKS  R++IVG L+VK ++ + E   K  K+   LS   D  YVQ
Sbjct: 361  SFPHKRPTGQVVAVVEKSLVRDSIVGLLDVKGWIHYKESDPKRCKSPLSLS---DDEYVQ 420

Query: 421  LMPNDARFPIMIVLARDLPDCIKKRLENGDVTVESELVAARIDEWVEESSAPRAQVLHVL 480
            LMP D RFP +IV    LP  I+ RLEN D  +E+ELVAA+I +W E S  P AQ+ H+ 
Sbjct: 421  LMPADPRFPKLIVPFHVLPGSIRARLENLDPNLEAELVAAQIVDWGEGSPFPVAQITHLF 480

Query: 481  GQGSKIESHIDAILFENAIRKCEFSQDSLSCLPRTPWKIPHEELQCRRDLRNLCIFTIDP 540
            G+GS++E  I+AIL++N++   +FS  SL+ LPR PW++P EE+Q R+DLR+LC+ TIDP
Sbjct: 481  GRGSELEPQINAILYQNSVCDSDFSPGSLTSLPRVPWEVPEEEVQRRKDLRDLCVLTIDP 540

Query: 541  SSVLDLDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIRSTSVYLLQHKIPML 600
            S+  DLDDALSVQ L  G FRVG+HIADVS+FVLP+TALD EA+ RSTSVYL+Q KI ML
Sbjct: 541  STATDLDDALSVQSLPGGFFRVGVHIADVSYFVLPETALDTEARFRSTSVYLMQRKISML 600

Query: 601  PPLLSENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSCCKLSYEHAQDIIDGL 660
            PPLLSEN+ SL+PG DRLAFS+  DL+  G V D WI RT+I SCCKLSY+HAQDIIDG 
Sbjct: 601  PPLLSENVGSLSPGADRLAFSILWDLNREGDVIDRWIGRTIIRSCCKLSYDHAQDIIDGK 660

Query: 661  IDSDSSKILGNCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRDGALRLENSKIVYLYD 720
             D   +      P LH  F W DV  SVK L EIS TL++KRFR+GAL+LENSK V+L+D
Sbjct: 661  SDVAEN----GWPALHGSFKWCDVTRSVKQLSEISTTLRQKRFRNGALQLENSKPVFLFD 720

Query: 721  EYGIPYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTYPNSALLRRQPEPILRKLREFE 780
            E+G+PYD     +K SNFLVEEFMLLAN T AEVIS+ Y  S+LLRR PEP  RKL+EFE
Sbjct: 721  EHGVPYDFVTCSRKGSNFLVEEFMLLANMTAAEVISQAYRASSLLRRHPEPNTRKLKEFE 780

Query: 781  SFCSKHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYATRPMQLATYFCNGELKD 840
             FCSKHG++LD SSS + Q SLE+I   L DD    +I+ +YA +PMQLA+YFC G LKD
Sbjct: 781  GFCSKHGMDLDISSSGQLQDSLEKITGNLKDDSVFVDILNNYAIKPMQLASYFCTGNLKD 840

Query: 841  G-ENGSHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEAEELYLKHQGIIPKVNSDEQM 900
                  HYALA PLYTHFTSPLRRYPDIVVHR LAAALEAEELY K +    +   DE  
Sbjct: 841  SVAEWGHYALAVPLYTHFTSPLRRYPDIVVHRALAAALEAEELYSKQK----QTAIDEGR 900

Query: 901  RCFTGIYFDKDAADSSEGREALSSAASRHGLPCSKLLSDVALHCNNRKLASKHVADACNK 960
             CFTGI+F+KDAA+S EG+EALS AA +HG+P +++LSDVA +CN RKLA++ V DAC+K
Sbjct: 901  SCFTGIHFNKDAAESIEGKEALSVAALKHGVPSTEILSDVAAYCNERKLAARKVRDACDK 960

Query: 961  LYMWALLKEKKVLFSDARVLGLGPRFMSVYIQKLAIERRIYYDEVEGLEVEWLDATSTLV 1020
            LY W +LK+K++   +ARV+ LG RFM+VYI KL IERRIYYD++EGL  +WL+ATSTL+
Sbjct: 961  LYTWFVLKQKEIFPCEARVMNLGSRFMTVYISKLGIERRIYYDQIEGLCADWLEATSTLI 1020

Query: 1021 LSFFGTRRPHRNRGSSIKWKALEDVALVISPCDLNVKQRTLGGMSLSESGGAGTGGASVE 1080
            +      + +  RG    +K +++   ++SPC++ V + +   +  +ES  A        
Sbjct: 1021 VD-----KLYSKRGGRGFFKPMKEAVYLVSPCEVCVAKCSALSVHDTESPEA-------- 1054

Query: 1081 QEPNLKSHVSDTGIDPAVFPLTVRLLSTIPVALHAVGGDDGPIDIGVRLYTSSY 1129
                    VS   + PAVFPLT++L STIPV LHAVGGDDGP+DIG RLY SSY
Sbjct: 1081 --------VSIDEVAPAVFPLTIQLFSTIPVVLHAVGGDDGPLDIGARLYMSSY 1054

BLAST of Sed0025890.3 vs. TAIR 10
Match: AT2G17510.1 (ribonuclease II family protein )

HSP 1 Score: 290.4 bits (742), Expect = 6.1e-78
Identity = 209/719 (29.07%), Postives = 330/719 (45.90%), Query Frame = 0

Query: 157 DANATVNNTQRKYFTSHWAMDDVNERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILI 216
           DA+ +  + ++  +  H  M ++   L +GI  +   RVN  N  EAY   + +  +I+I
Sbjct: 203 DADDSRPSKRKLIYQEHKPMSEITAGLHRGIYHQGKLRVNRFNPYEAYVGSESIGEEIII 262

Query: 217 NGIASQNRALEGDIVAIMVDPFTSWTMMKGTCEAHNNKHSMEDANLPAEETEKNSHNCKD 276
            G ++ NRA +GDIVA+ + P   W   K    A  +           +E +   H   D
Sbjct: 263 YGRSNMNRAFDGDIVAVELLPRDQWQDEKALSIAEED-----------DEEDDTVHLAPD 322

Query: 277 KNKVNSDVKSDSYRSSSSSDKRCCSEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHY 336
              V+   ++ +    +S DK                                       
Sbjct: 323 --NVDDAPRTSNLSHETSGDKNAA------------------------------------ 382

Query: 337 SSIQDDVSMAIGRICTLINLYPSKRPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYT 396
                                   RP+GRVV +I +                 ++H  Y 
Sbjct: 383 ----------------------PVRPSGRVVGVIRR-----------------NWHS-YC 442

Query: 397 KEMKTKSCLSSSHDSGYVQLMPNDARFPIMIVLARDLPDCIKKRLENGDVTVESELVAAR 456
             ++  S  + S  + +   +  D R P + +  R L + +  R            +   
Sbjct: 443 GSLEPMSLPAGSGGTAHALFVSKDRRIPKIRINTRQLQNLLDMR------------IVVA 502

Query: 457 IDEWVEESSAPRAQVLHVLGQGSKIESHIDAILFENAIRKCEFSQDSLSCLPRTPWKIPH 516
           +D W  +S  P    +  +G+    E+  + +L EN +    FS   L+CLP  PW +  
Sbjct: 503 VDSWDRQSRYPSGHYVRPIGKIGDKETETEVVLIENDVDYSPFSSQVLACLPPLPWSVSS 562

Query: 517 EELQ--CRRDLRNLCIFTIDPSSVLDLDDALSVQKLANGIFRVGIHIADVSHFVLPDTAL 576
           E++    R+DLR+L +F++DP    D+DDAL    L NG F +G+HIADV++FV P T L
Sbjct: 563 EDVSNPVRQDLRHLLVFSVDPPGCKDIDDALHCTSLPNGNFELGVHIADVTNFVHPGTPL 622

Query: 577 DKEAQIRSTSVYLLQHKIPMLPPLLSENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDR 636
           D EA  R TSVYL++ +I MLP  L+E++ SL   V+RLAFS+  ++     +      +
Sbjct: 623 DDEASKRGTSVYLVERRIDMLPKPLTEDICSLRADVERLAFSVIWEMSPDAEIISTRFTK 682

Query: 637 TVICSCCKLSYEHAQDIIDGLIDSDSSKILGNCPQLHSQFSWLDVISSVKILHEISKTLK 696
           ++I S   LSY  AQ  +D    +DS                  + + ++ ++ ++K ++
Sbjct: 683 SIIKSSAALSYIEAQARMDDSRLTDS------------------LTTDLRNMNTLAKIMR 742

Query: 697 EKRFRDGALRLENSKIVYLYD-EYGIPYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRT 756
           ++R   GAL L ++++ +  D E   P +   Y+  ++N +VEEFML AN +VA  I + 
Sbjct: 743 QRRIDRGALTLASAEVKFDIDPENHDPLNIGMYQILEANQMVEEFMLAANVSVAGQILKL 798

Query: 757 YPNSALLRRQPEPILRKLREFESFCSKHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNI 816
           +P+ +LLRR P P    L       +  GL LD SSS     SL++    + +DP    +
Sbjct: 803 FPSCSLLRRHPTPTREMLEPLLRTAAAIGLTLDVSSSKALADSLDR---AVGEDPYFNKL 798

Query: 817 VISYATRPMQLATYFCNGELKDGENGSHYALAAPLYTHFTSPLRRYPDIVVHRTLAAAL 873
           +   ATR M  A YFC+G+L   E   HY LAAPLYTHFTSP+RRY D+ VHR LAA+L
Sbjct: 863 IRILATRCMTQAVYFCSGDLSPPEY-HHYGLAAPLYTHFTSPIRRYADVFVHRLLAASL 798

BLAST of Sed0025890.3 vs. TAIR 10
Match: AT2G17510.2 (ribonuclease II family protein )

HSP 1 Score: 268.9 bits (686), Expect = 1.9e-71
Identity = 209/753 (27.76%), Postives = 333/753 (44.22%), Query Frame = 0

Query: 157 DANATVNNTQRKYFTSHWAMDDVNERLQKGIIFKALFRVNAHNRLEAYCKIDGLPVDILI 216
           DA+ +  + ++  +  H  M ++   L +GI  +   RVN  N  EAY   + +  +I+I
Sbjct: 248 DADDSRPSKRKLIYQEHKPMSEITAGLHRGIYHQGKLRVNRFNPYEAYVGSESIGEEIII 307

Query: 217 NGIASQNRALEGDIVAIMVDPFTSWTMMKGTCEAHNNKHSMEDANLPAEETEKNSHNCKD 276
            G ++ NRA +GDIVA+ + P   W   K    A  +           +E +   H   D
Sbjct: 308 YGRSNMNRAFDGDIVAVELLPRDQWQDEKALSIAEED-----------DEEDDTVHLAPD 367

Query: 277 KNKVNSDVKSDSYRSSSSSDKRCCSEEKVLDGTACDDLLSNYEYCDAYHPSAVGPSQAHY 336
              V+   ++ +    +S DK                                       
Sbjct: 368 --NVDDAPRTSNLSHETSGDKNAA------------------------------------ 427

Query: 337 SSIQDDVSMAIGRICTLINLYPSKRPTGRVVAIIEKSRPRETIVGHLNVKKFLSFHEIYT 396
                                   RP+GRVV +I +                 ++H  Y 
Sbjct: 428 ----------------------PVRPSGRVVGVIRR-----------------NWHS-YC 487

Query: 397 KEMKTKSCLSSSHDSGYVQLMPNDARFPIMIVLARDLPDCIKKRLENGDVTVESELVAAR 456
             ++  S  + S  + +   +  D R P + +  R L + +  R            +   
Sbjct: 488 GSLEPMSLPAGSGGTAHALFVSKDRRIPKIRINTRQLQNLLDMR------------IVVA 547

Query: 457 IDEWVEESSAPRAQVLHVLGQ------GSKIESHID----------------AILFENAI 516
           +D W  +S  P    +  +G+       +++  HI+                 +L EN +
Sbjct: 548 VDSWDRQSRYPSGHYVRPIGKIGDKETETEVRDHINLFDSILVGVRWARVGKVVLIENDV 607

Query: 517 RKCEFSQDSLSCLPRTPWKIPHEELQ--CRRDLRNLCIFTIDPSSVLDLDDALSVQKLAN 576
               FS   L+CLP  PW +  E++    R+DLR+L +F++DP    D+DDAL    L N
Sbjct: 608 DYSPFSSQVLACLPPLPWSVSSEDVSNPVRQDLRHLLVFSVDPPGCKDIDDALHCTSLPN 667

Query: 577 GIFRVGI------------HIADVSHFVLPDTALDKEAQIRSTSVYLLQHKIPMLPPLLS 636
           G F +G+            +IADV++FV P T LD EA  R TSVYL++ +I MLP  L+
Sbjct: 668 GNFELGVRILESSDSHKYDYIADVTNFVHPGTPLDDEASKRGTSVYLVERRIDMLPKPLT 727

Query: 637 ENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSCCKLSYEHAQDIIDGLIDSDS 696
           E++ SL   V+RLAFS+  ++     +      +++I S   LSY  AQ  +D    +DS
Sbjct: 728 EDICSLRADVERLAFSVIWEMSPDAEIISTRFTKSIIKSSAALSYIEAQARMDDSRLTDS 787

Query: 697 SKILGNCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRDGALRLENSKIVYLYD-EYGI 756
                             + + ++ ++ ++K ++++R   GAL L ++++ +  D E   
Sbjct: 788 ------------------LTTDLRNMNTLAKIMRQRRIDRGALTLASAEVKFDIDPENHD 847

Query: 757 PYDSTFYEQKDSNFLVEEFMLLANRTVAEVISRTYPNSALLRRQPEPILRKLREFESFCS 816
           P +   Y+  ++N +VEEFML AN +VA  I + +P+ +LLRR P P    L       +
Sbjct: 848 PLNIGMYQILEANQMVEEFMLAANVSVAGQILKLFPSCSLLRRHPTPTREMLEPLLRTAA 877

Query: 817 KHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYATRPMQLATYFCNGELKDGENG 873
             GL LD SSS     SL++    + +DP    ++   ATR M  A YFC+G+L   E  
Sbjct: 908 AIGLTLDVSSSKALADSLDR---AVGEDPYFNKLIRILATRCMTQAVYFCSGDLSPPEY- 877

BLAST of Sed0025890.3 vs. TAIR 10
Match: AT5G02250.1 (Ribonuclease II/R family protein )

HSP 1 Score: 95.9 bits (237), Expect = 2.2e-19
Identity = 94/379 (24.80%), Postives = 154/379 (40.63%), Query Frame = 0

Query: 522 RRDLRNLCIFTIDPSSVLDLDDALSVQKLANGIFRVGIHIADVSHFVLPDTALDKEAQIR 581
           R DL +L ++ ID     +LDDALS  +L +G  ++ IH+AD + +V P + +D+EA+ R
Sbjct: 399 RIDLTHLKVYAIDVDEADELDDALSATRLQDGRIKIWIHVADPARYVTPGSKVDREARRR 458

Query: 582 STSVYLLQHKIPMLPPLLSENMVSLNPGVDRLAFSLFLDLDHCGGVKDYWIDRTVICSCC 641
            TSV+L     PM P  L+   +SL  G +  A S+ + L   G + +Y +D ++I    
Sbjct: 459 GTSVFLPTATYPMFPEKLAMEGMSLRQGENCNAVSVSVVLRSDGCITEYSVDNSIIRPTY 518

Query: 642 KLSYEHAQDIIDGLIDSDSS-KILGNCPQLHSQFSWLDVISSVKILHEISKTLKEKRFRD 701
            L+YE A +++   ++ ++  K+L     + SQ+           L    K +  +    
Sbjct: 519 MLTYESASELLHLNLEEEAELKLLSEAAFIRSQWRREQGAVDTTTLETRIKVVNPE---- 578

Query: 702 GALRLENSKIVYLYDEYGIPYDSTFYEQKD-SNFLVEEFMLLANRTVAEVISRTYPNSAL 761
                +   ++ LY E           Q D +  LV E M+L    VA            
Sbjct: 579 -----DPEPLINLYVE----------NQADLAMRLVFEMMILCGEVVA------------ 638

Query: 762 LRRQPEPILRKLREFESFCSKHGLELDTSSSVRFQQSLEQIRIKLHDDPSLFNIVISYAT 821
                           +F S+H + L         +   Q  I    D S F  +     
Sbjct: 639 ----------------TFGSQHNIPLP-------YRGQPQSNI----DVSAFAHLPEGPV 698

Query: 822 RPMQLATYFCNGELKDGENGSHYALAAPLYTHFTSPLRRYPDIVVHRTLAAALEAEELYL 881
           R   +       E+       H  L  P Y  FTSP+RRY D+  H  + A L   + + 
Sbjct: 699 RSSSIVKVMRAAEMNFRCPVRHGVLGIPGYVQFTSPIRRYMDLTAHYQIKAFLRGGDNFP 719

Query: 882 ----KHQGIIPKVNSDEQM 895
               + +GI   VN   ++
Sbjct: 759 FSAGELEGIAASVNMQSKV 719