MS012090 (gene) Bitter gourd (TR) v1

BLAST of MS012090 vs. NCBI nr
Match: XP_023890881.1 (cell wall / vacuolar inhibitor of fructosidase 1-like [Quercus suber])

HSP 1 Score: 75.1 bits (183), Expect = 4.7e-10
Identity = 43/95 (45.26%), Postives = 58/95 (61.05%), Query Frame = 0

Query: 28  AANLITETCSKTSHVDLCISSLGVDPRSPTADVRRLAEIILKETLANAQEILAHRVESFI 87
           + NLI  TC KTSH DLC+SSL  +P+S TADV+ LA+I+    LANA + L + +E  I
Sbjct: 96  SGNLIEHTCKKTSHYDLCLSSLQSNPQSSTADVKGLAQIMADILLANATDALNY-IEGLI 155

Query: 88  NQTTDPAVKECLAACKVLYRLVTNSTIPSAMWQLS 123
            Q  +P ++  L  C  LY  V   T+P A+  LS
Sbjct: 156 KQAPEPELERSLTYCAELYIPVVKYTLPQAIDALS 189

BLAST of MS012090 vs. NCBI nr
Match: XP_030975931.1 (cell wall / vacuolar inhibitor of fructosidase 1-like [Quercus lobata])

HSP 1 Score: 73.9 bits (180), Expect = 1.0e-09
Identity = 42/95 (44.21%), Postives = 58/95 (61.05%), Query Frame = 0

Query: 28  AANLITETCSKTSHVDLCISSLGVDPRSPTADVRRLAEIILKETLANAQEILAHRVESFI 87
           + NLI  TC KTSH DLC+SSL  +P+S TADV+ LA+I+    LAN  + L + +E  I
Sbjct: 34  SGNLIEHTCKKTSHYDLCLSSLQSNPQSATADVKGLAQIMADILLANVTDTLNY-IEGLI 93

Query: 88  NQTTDPAVKECLAACKVLYRLVTNSTIPSAMWQLS 123
            Q+ +P ++  L  C  LY  V   T+P A+  LS
Sbjct: 94  KQSPEPELERSLTYCAELYIPVVKYTLPQAIDALS 127

BLAST of MS012090 vs. NCBI nr
Match: XP_017969808.1 (PREDICTED: pectinesterase inhibitor [Theobroma cacao])

HSP 1 Score: 73.9 bits (180), Expect = 1.0e-09
Identity = 44/110 (40.00%), Postives = 72/110 (65.45%), Query Frame = 0

Query: 12  IVVSLLLVVNHSSSLVAANLITETCSKTSHVDLCISSLGVDPRSPTADVRRLAEIILKET 71
           ++VSLL +++     V A++I +TC++TS  +LC+S+L  DPRS TAD + LA I+L  T
Sbjct: 12  LIVSLLFLMS-----VRADMIDDTCNRTSDTELCLSTLRSDPRSSTADAKGLAHIVLDIT 71

Query: 72  LANAQEILAHRVESFINQTTDPAVKECLAACKVLYRLVTNSTIPSAMWQL 122
           L  A++ LA +++  +++TTDPA+K+ L  C   Y   T   +P A+ +L
Sbjct: 72  LKKAKDNLA-QIDQLLSRTTDPALKDVLTICSQEYGNATVVILPRAIKKL 115

BLAST of MS012090 vs. NCBI nr
Match: KAG5237625.1 (pectinesterase inhibitor [Salix suchowensis])

HSP 1 Score: 73.6 bits (179), Expect = 1.4e-09
Identity = 45/117 (38.46%), Postives = 73/117 (62.39%), Query Frame = 0

Query: 7   IKWAAIVVSLLLV--VNHSSSLVAANLITETCSKTSHVDLCISSLGVDPRSPTADVRRLA 66
           +K+ A +   LLV  ++  ++LV A+L+ ETC KT + DLC+ +L  +PRS +ADV+ LA
Sbjct: 1   MKFLAWLPLFLLVNFLHQPTALVGADLVQETCQKTRYPDLCVKTLKSNPRSSSADVKGLA 60

Query: 67  EIILKETLANAQEILAHRVESFINQTTDPAVKECLAACKVLYRLVTNSTIPSAMWQL 122
            I+L+  LAN++  LA +VE  + ++ D ++K+CL  C   Y    N   P+A+  L
Sbjct: 61  HIMLEANLANSKVSLA-KVEKLLKESGDKSLKKCLDDCAEQYDTAANEDFPTAIQSL 116

BLAST of MS012090 vs. NCBI nr
Match: XP_007221576.1 (cell wall / vacuolar inhibitor of fructosidase 2 [Prunus persica] >ONI14778.1 hypothetical protein PRUPE_3G007700 [Prunus persica])

HSP 1 Score: 73.6 bits (179), Expect = 1.4e-09
Identity = 44/102 (43.14%), Postives = 63/102 (61.76%), Query Frame = 0

Query: 7   IKWAAIVVSLLLVVNHSSSLVAANLITETCSKTSHVDLCISSLGVDPRSPTADVRRLAEI 66
           +KW  I    L V++ S   V+ +L+ ETC KT++ DLC+SSL  DPRS TADV+ LA I
Sbjct: 4   LKW-IIACLTLFVLSQSRGSVSTDLVEETCHKTTNYDLCVSSLKSDPRSSTADVKGLARI 63

Query: 67  ILKETLANAQEILAHRVESFINQTTDPAVKECLAACKVLYRL 109
            L +TL N+ +  A R+    N+T+D  +++ L  CK  Y L
Sbjct: 64  ALDQTLTNSVDTQA-RIARLFNETSDEYIRKGLGTCKDEYDL 103

BLAST of MS012090 vs. ExPASy Swiss-Prot
Match: O49603 (Cell wall / vacuolar inhibitor of fructosidase 2 OS=Arabidopsis thaliana OX=3702 GN=C/VIF2 PE=1 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 6.2e-05
Identity = 35/110 (31.82%), Postives = 56/110 (50.91%), Query Frame = 0

Query: 10  AAIVVSLLLV--VNHSSSLVAA-----NLITETCSKTSHVDLCISSLGVDPRSPTADVRR 69
           A+ ++ LLLV     +S+L++A      +I  TC  T++   C+S+L  DPRSPTAD + 
Sbjct: 2   ASSLIFLLLVTLTFSASTLISAKSNTTTIIESTCKTTNYYKFCVSALKSDPRSPTADTKG 61

Query: 70  LAEIILKETLANAQEILAHRVESFINQTTDPAVKECLAACKVLYRLVTNS 113
           LA I++   + NA     +   +      D  +K+ L  C   Y L  +S
Sbjct: 62  LASIMVGVGMTNATSTANYIAGNLSATVKDTVLKKVLQDCSEKYALAADS 111

BLAST of MS012090 vs. ExPASy Swiss-Prot
Match: Q0J8J8 (Pectinesterase inhibitor 28 OS=Oryza sativa subsp. japonica OX=39947 GN=PMEI28 PE=2 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 1.4e-04
Identity = 32/87 (36.78%), Postives = 43/87 (49.43%), Query Frame = 0

Query: 28  AANLITETCSKTSHVDLCISSLGVDPRSPTADVRRLAEIILKETLANAQEILAHRVESFI 87
           AA L+  TC+ T++ D+C+S+L  DP S TADVR L+ I +    ANA    A    +  
Sbjct: 58  AAALVRATCNSTAYYDVCVSALAADPSSTTADVRGLSAIAVSVAAANASG-AAQAAAALA 117

Query: 88  NQTT--------DPAVKECLAACKVLY 107
           N T         D  V+  L AC   Y
Sbjct: 118 NGTAPLAAAAAGDGTVQALLRACAGKY 143

BLAST of MS012090 vs. ExPASy Swiss-Prot
Match: P83326 (Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2)

HSP 1 Score: 46.2 bits (108), Expect = 3.1e-04
Identity = 35/104 (33.65%), Postives = 56/104 (53.85%), Query Frame = 0

Query: 10  AAIVVSLLLVV------NHSSSLVAAN-LITETCSKTSHVDLCISSLGVDPRSPTADVRR 69
           +++ VSLLLV+      +   S+ A N LI+E C KT +  LC+ +L  DPRS + D++ 
Sbjct: 8   SSLFVSLLLVILFISPLSQRPSVKAENHLISEICPKTRNPSLCLQALESDPRSASKDLKG 67

Query: 70  LAEIILKETLANAQEILAHRVESFINQTTDPAVKECLAACKVLY 107
           L +  +    A+A++  +  + S  NQ TDP +K     C   Y
Sbjct: 68  LGQFSIDIAQASAKQ-TSKIIASLTNQATDPKLKGRYETCSENY 110

BLAST of MS012090 vs. ExPASy TrEMBL
Match: A0A7N2LXR4 (PMEI domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 5.1e-10
Identity = 42/95 (44.21%), Postives = 58/95 (61.05%), Query Frame = 0

Query: 28  AANLITETCSKTSHVDLCISSLGVDPRSPTADVRRLAEIILKETLANAQEILAHRVESFI 87
           + NLI  TC KTSH DLC+SSL  +P+S TADV+ LA+I+    LAN  + L + +E  I
Sbjct: 34  SGNLIEHTCKKTSHYDLCLSSLQSNPQSATADVKGLAQIMADILLANVTDTLNY-IEGLI 93

Query: 88  NQTTDPAVKECLAACKVLYRLVTNSTIPSAMWQLS 123
            Q+ +P ++  L  C  LY  V   T+P A+  LS
Sbjct: 94  KQSPEPELERSLTYCAELYIPVVKYTLPQAIDALS 127

BLAST of MS012090 vs. ExPASy TrEMBL
Match: M5XUJ1 (PMEI domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_3G007700 PE=4 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 6.6e-10
Identity = 44/102 (43.14%), Postives = 63/102 (61.76%), Query Frame = 0

Query: 7   IKWAAIVVSLLLVVNHSSSLVAANLITETCSKTSHVDLCISSLGVDPRSPTADVRRLAEI 66
           +KW  I    L V++ S   V+ +L+ ETC KT++ DLC+SSL  DPRS TADV+ LA I
Sbjct: 4   LKW-IIACLTLFVLSQSRGSVSTDLVEETCHKTTNYDLCVSSLKSDPRSSTADVKGLARI 63

Query: 67  ILKETLANAQEILAHRVESFINQTTDPAVKECLAACKVLYRL 109
            L +TL N+ +  A R+    N+T+D  +++ L  CK  Y L
Sbjct: 64  ALDQTLTNSVDTQA-RIARLFNETSDEYIRKGLGTCKDEYDL 103

BLAST of MS012090 vs. ExPASy TrEMBL
Match: A0A5N5L7V8 (PMEI domain-containing protein OS=Salix brachista OX=2182728 GN=DKX38_016388 PE=4 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 6.6e-10
Identity = 44/117 (37.61%), Postives = 72/117 (61.54%), Query Frame = 0

Query: 7   IKWAAIVVSLLLV--VNHSSSLVAANLITETCSKTSHVDLCISSLGVDPRSPTADVRRLA 66
           +K+ A +   LLV  ++  ++LV A+L+ ETC KT + DLC+ +L  +PRS TADV+ L 
Sbjct: 1   MKFLACLPMFLLVNFLDQPTTLVGADLVQETCQKTRYPDLCVKTLKSNPRSSTADVKGLV 60

Query: 67  EIILKETLANAQEILAHRVESFINQTTDPAVKECLAACKVLYRLVTNSTIPSAMWQL 122
            I+L+  LAN++  LA +V+  + ++ D ++K+CL  C   Y    N   P+A+  L
Sbjct: 61  HIMLEANLANSKVTLA-KVDKLLKESGDKSLKKCLDDCAEEYDTAANEDFPTAIQSL 116

BLAST of MS012090 vs. ExPASy TrEMBL
Match: A0A5E4F258 (PREDICTED: cell wall OS=Prunus dulcis OX=3755 GN=ALMOND_2B015884 PE=4 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 1.5e-09
Identity = 44/102 (43.14%), Postives = 62/102 (60.78%), Query Frame = 0

Query: 7   IKWAAIVVSLLLVVNHSSSLVAANLITETCSKTSHVDLCISSLGVDPRSPTADVRRLAEI 66
           +KW  I    L V++ S   V+ + I ETC KT++ DLC+SSL  DPRS TADV+ LA I
Sbjct: 4   LKW-IIACLTLFVLSQSRGSVSTDFIQETCHKTTNYDLCVSSLKSDPRSSTADVKGLARI 63

Query: 67  ILKETLANAQEILAHRVESFINQTTDPAVKECLAACKVLYRL 109
            L +TL N+ +  A R+    N+T+D  +++ L  CK  Y L
Sbjct: 64  ALDQTLTNSVDTQA-RIARLFNETSDEYIRKGLGTCKDEYDL 103

BLAST of MS012090 vs. ExPASy TrEMBL
Match: A0A061DR99 (PMEI domain-containing protein OS=Theobroma cacao OX=3641 GN=TCM_004852 PE=4 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 2.5e-09
Identity = 44/115 (38.26%), Postives = 75/115 (65.22%), Query Frame = 0

Query: 7   IKWAAIVVSLLLVVNHSSSLVAANLITETCSKTSHVDLCISSLGVDPRSPTADVRRLAEI 66
           +K A +V+ ++L++   S  V A++I +TC++TS  +LC+S+L  DPRS TAD + LA I
Sbjct: 4   LKVADLVLLIVLLLFLMS--VRADMIDDTCNRTSDTELCLSTLRSDPRSSTADAKGLAHI 63

Query: 67  ILKETLANAQEILAHRVESFINQTTDPAVKECLAACKVLYRLVTNSTIPSAMWQL 122
           +L  TL  A++ +A +++  +++TTDPA+K+ L  C   Y   T   +P A+ +L
Sbjct: 64  VLDITLKKAKDNVA-QIDQLLSRTTDPALKDVLTICSQEYGNATVVILPRAIQKL 115

BLAST of MS012090 vs. TAIR 10
Match: AT5G64620.1 (cell wall / vacuolar inhibitor of fructosidase 2 )

HSP 1 Score: 48.5 bits (114), Expect = 4.4e-06
Identity = 35/110 (31.82%), Postives = 56/110 (50.91%), Query Frame = 0

Query: 10  AAIVVSLLLV--VNHSSSLVAA-----NLITETCSKTSHVDLCISSLGVDPRSPTADVRR 69
           A+ ++ LLLV     +S+L++A      +I  TC  T++   C+S+L  DPRSPTAD + 
Sbjct: 2   ASSLIFLLLVTLTFSASTLISAKSNTTTIIESTCKTTNYYKFCVSALKSDPRSPTADTKG 61

Query: 70  LAEIILKETLANAQEILAHRVESFINQTTDPAVKECLAACKVLYRLVTNS 113
           LA I++   + NA     +   +      D  +K+ L  C   Y L  +S
Sbjct: 62  LASIMVGVGMTNATSTANYIAGNLSATVKDTVLKKVLQDCSEKYALAADS 111

BLAST of MS012090 vs. TAIR 10
Match: AT1G47960.1 (cell wall / vacuolar inhibitor of fructosidase 1 )

HSP 1 Score: 43.1 bits (100), Expect = 1.8e-04
Identity = 31/122 (25.41%), Postives = 61/122 (50.00%), Query Frame = 0

Query: 1   LKMFPLIKWAAIVVSLLLVVNHSSSLVAANLITETCSKTSHVDLCISSLGVDPRSPTADV 60
           +KM  ++    +++ ++++V+  S      +I  TC +T   +LC+S L  DPR  +AD 
Sbjct: 1   MKMMKVMMLIVMMMMVMVMVSEGS------IIEPTCKETPDFNLCVSLLNSDPRGSSADT 60

Query: 61  RRLAEIILKETLANAQEILAHRVESFINQTTDPAVKECLAACKVLYRLVTNSTIPSAMWQ 120
             LA I++ +    A + L + +     +   P +K  L  C   Y+ + N+ +P A+  
Sbjct: 61  SGLALILIDKIKGLATKTL-NEINGLYKKR--PELKRALDECSRRYKTILNADVPEAIEA 113

Query: 121 LS 123
           +S
Sbjct: 121 IS 113