MS012090.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS012090.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	PMEI domain-containing protein
Location	scaffold708: 211803 .. 212168 (-)
Sequence length	366
RNA-Seq Expression	MS012090.1
Synteny	MS012090.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTAAAGATGTTCCCTCTCATCAAGTGGGCTGCCATTGTTGTTTCTCTGCTACTTGTTGTTAACCACTCTTCATCTTTGGTTGCAGCCAATCTCATTACAGAAACATGTTCGAAGACATCCCATGTTGACCTCTGCATATCGAGCCTTGGGGTCGACCCTCGAAGCCCGACGGCCGATGTTAGGCGTCTTGCCGAGATCATCCTCAAAGAGACATTAGCGAACGCCCAAGAAATTCTGGCACATAGAGTTGAGAGCTTCATCAACCAGACGACAGATCCTGCAGTGAAGGAATGTCTCGCTGCCTGCAAAGTGCTGTATCGACTTGTAACCAACTCGACGATCCCAAGCGCAATGTGGCAACTCTCG

mRNA sequence

TTAAAGATGTTCCCTCTCATCAAGTGGGCTGCCATTGTTGTTTCTCTGCTACTTGTTGTTAACCACTCTTCATCTTTGGTTGCAGCCAATCTCATTACAGAAACATGTTCGAAGACATCCCATGTTGACCTCTGCATATCGAGCCTTGGGGTCGACCCTCGAAGCCCGACGGCCGATGTTAGGCGTCTTGCCGAGATCATCCTCAAAGAGACATTAGCGAACGCCCAAGAAATTCTGGCACATAGAGTTGAGAGCTTCATCAACCAGACGACAGATCCTGCAGTGAAGGAATGTCTCGCTGCCTGCAAAGTGCTGTATCGACTTGTAACCAACTCGACGATCCCAAGCGCAATGTGGCAACTCTCG

Coding sequence (CDS)

TTAAAGATGTTCCCTCTCATCAAGTGGGCTGCCATTGTTGTTTCTCTGCTACTTGTTGTTAACCACTCTTCATCTTTGGTTGCAGCCAATCTCATTACAGAAACATGTTCGAAGACATCCCATGTTGACCTCTGCATATCGAGCCTTGGGGTCGACCCTCGAAGCCCGACGGCCGATGTTAGGCGTCTTGCCGAGATCATCCTCAAAGAGACATTAGCGAACGCCCAAGAAATTCTGGCACATAGAGTTGAGAGCTTCATCAACCAGACGACAGATCCTGCAGTGAAGGAATGTCTCGCTGCCTGCAAAGTGCTGTATCGACTTGTAACCAACTCGACGATCCCAAGCGCAATGTGGCAACTCTCG

Protein sequence

LKMFPLIKWAAIVVSLLLVVNHSSSLVAANLITETCSKTSHVDLCISSLGVDPRSPTADVRRLAEIILKETLANAQEILAHRVESFINQTTDPAVKECLAACKVLYRLVTNSTIPSAMWQLS

Homology

[Close]

BLAST of MS012090.1 vs. NCBI nr
Match: XP_023890881.1 (cell wall / vacuolar inhibitor of fructosidase 1-like [Quercus suber])

HSP 1 Score: 75.1 bits (183), Expect = 4.7e-10
Identity = 43/95 (45.26%), Postives = 58/95 (61.05%), Query Frame = 0


Query: 28  AANLITETCSKTSHVDLCISSLGVDPRSPTADVRRLAEIILKETLANAQEILAHRVESFI 87
           + NLI  TC KTSH DLC+SSL  +P+S TADV+ LA+I+    LANA + L + +E  I
Sbjct: 96  SGNLIEHTCKKTSHYDLCLSSLQSNPQSSTADVKGLAQIMADILLANATDALNY-IEGLI 155

Query: 88  NQTTDPAVKECLAACKVLYRLVTNSTIPSAMWQLS 123
            Q  +P ++  L  C  LY  V   T+P A+  LS
Sbjct: 156 KQAPEPELERSLTYCAELYIPVVKYTLPQAIDALS 189

[Close]

BLAST of MS012090.1 vs. NCBI nr
Match: XP_030975931.1 (cell wall / vacuolar inhibitor of fructosidase 1-like [Quercus lobata])

HSP 1 Score: 73.9 bits (180), Expect = 1.0e-09
Identity = 42/95 (44.21%), Postives = 58/95 (61.05%), Query Frame = 0


Query: 28  AANLITETCSKTSHVDLCISSLGVDPRSPTADVRRLAEIILKETLANAQEILAHRVESFI 87
           + NLI  TC KTSH DLC+SSL  +P+S TADV+ LA+I+    LAN  + L + +E  I
Sbjct: 34  SGNLIEHTCKKTSHYDLCLSSLQSNPQSATADVKGLAQIMADILLANVTDTLNY-IEGLI 93

Query: 88  NQTTDPAVKECLAACKVLYRLVTNSTIPSAMWQLS 123
            Q+ +P ++  L  C  LY  V   T+P A+  LS
Sbjct: 94  KQSPEPELERSLTYCAELYIPVVKYTLPQAIDALS 127

[Close]

BLAST of MS012090.1 vs. NCBI nr
Match: XP_017969808.1 (PREDICTED: pectinesterase inhibitor [Theobroma cacao])

HSP 1 Score: 73.9 bits (180), Expect = 1.0e-09
Identity = 44/110 (40.00%), Postives = 72/110 (65.45%), Query Frame = 0


Query: 12  IVVSLLLVVNHSSSLVAANLITETCSKTSHVDLCISSLGVDPRSPTADVRRLAEIILKET 71
           ++VSLL +++     V A++I +TC++TS  +LC+S+L  DPRS TAD + LA I+L  T
Sbjct: 12  LIVSLLFLMS-----VRADMIDDTCNRTSDTELCLSTLRSDPRSSTADAKGLAHIVLDIT 71

Query: 72  LANAQEILAHRVESFINQTTDPAVKECLAACKVLYRLVTNSTIPSAMWQL 122
           L  A++ LA +++  +++TTDPA+K+ L  C   Y   T   +P A+ +L
Sbjct: 72  LKKAKDNLA-QIDQLLSRTTDPALKDVLTICSQEYGNATVVILPRAIKKL 115

[Close]

BLAST of MS012090.1 vs. NCBI nr
Match: KAG5237625.1 (pectinesterase inhibitor [Salix suchowensis])

HSP 1 Score: 73.6 bits (179), Expect = 1.4e-09
Identity = 45/117 (38.46%), Postives = 73/117 (62.39%), Query Frame = 0


Query: 7   IKWAAIVVSLLLV--VNHSSSLVAANLITETCSKTSHVDLCISSLGVDPRSPTADVRRLA 66
           +K+ A +   LLV  ++  ++LV A+L+ ETC KT + DLC+ +L  +PRS +ADV+ LA
Sbjct: 1   MKFLAWLPLFLLVNFLHQPTALVGADLVQETCQKTRYPDLCVKTLKSNPRSSSADVKGLA 60

Query: 67  EIILKETLANAQEILAHRVESFINQTTDPAVKECLAACKVLYRLVTNSTIPSAMWQL 122
            I+L+  LAN++  LA +VE  + ++ D ++K+CL  C   Y    N   P+A+  L
Sbjct: 61  HIMLEANLANSKVSLA-KVEKLLKESGDKSLKKCLDDCAEQYDTAANEDFPTAIQSL 116

[Close]

BLAST of MS012090.1 vs. NCBI nr
Match: XP_007221576.1 (cell wall / vacuolar inhibitor of fructosidase 2 [Prunus persica] >ONI14778.1 hypothetical protein PRUPE_3G007700 [Prunus persica])

HSP 1 Score: 73.6 bits (179), Expect = 1.4e-09
Identity = 44/102 (43.14%), Postives = 63/102 (61.76%), Query Frame = 0


Query: 7   IKWAAIVVSLLLVVNHSSSLVAANLITETCSKTSHVDLCISSLGVDPRSPTADVRRLAEI 66
           +KW  I    L V++ S   V+ +L+ ETC KT++ DLC+SSL  DPRS TADV+ LA I
Sbjct: 4   LKW-IIACLTLFVLSQSRGSVSTDLVEETCHKTTNYDLCVSSLKSDPRSSTADVKGLARI 63

Query: 67  ILKETLANAQEILAHRVESFINQTTDPAVKECLAACKVLYRL 109
            L +TL N+ +  A R+    N+T+D  +++ L  CK  Y L
Sbjct: 64  ALDQTLTNSVDTQA-RIARLFNETSDEYIRKGLGTCKDEYDL 103

[Close]

BLAST of MS012090.1 vs. ExPASy Swiss-Prot
Match: O49603 (Cell wall / vacuolar inhibitor of fructosidase 2 OS=Arabidopsis thaliana OX=3702 GN=C/VIF2 PE=1 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 6.2e-05
Identity = 35/110 (31.82%), Postives = 56/110 (50.91%), Query Frame = 0


Query: 10  AAIVVSLLLV--VNHSSSLVAA-----NLITETCSKTSHVDLCISSLGVDPRSPTADVRR 69
           A+ ++ LLLV     +S+L++A      +I  TC  T++   C+S+L  DPRSPTAD + 
Sbjct: 2   ASSLIFLLLVTLTFSASTLISAKSNTTTIIESTCKTTNYYKFCVSALKSDPRSPTADTKG 61

Query: 70  LAEIILKETLANAQEILAHRVESFINQTTDPAVKECLAACKVLYRLVTNS 113
           LA I++   + NA     +   +      D  +K+ L  C   Y L  +S
Sbjct: 62  LASIMVGVGMTNATSTANYIAGNLSATVKDTVLKKVLQDCSEKYALAADS 111

[Close]

BLAST of MS012090.1 vs. ExPASy Swiss-Prot
Match: Q0J8J8 (Pectinesterase inhibitor 28 OS=Oryza sativa subsp. japonica OX=39947 GN=PMEI28 PE=2 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 1.4e-04
Identity = 32/87 (36.78%), Postives = 43/87 (49.43%), Query Frame = 0


Query: 28  AANLITETCSKTSHVDLCISSLGVDPRSPTADVRRLAEIILKETLANAQEILAHRVESFI 87
           AA L+  TC+ T++ D+C+S+L  DP S TADVR L+ I +    ANA    A    +  
Sbjct: 58  AAALVRATCNSTAYYDVCVSALAADPSSTTADVRGLSAIAVSVAAANASG-AAQAAAALA 117

Query: 88  NQTT--------DPAVKECLAACKVLY 107
           N T         D  V+  L AC   Y
Sbjct: 118 NGTAPLAAAAAGDGTVQALLRACAGKY 143

[Close]

BLAST of MS012090.1 vs. ExPASy Swiss-Prot
Match: P83326 (Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2)

HSP 1 Score: 46.2 bits (108), Expect = 3.1e-04
Identity = 35/104 (33.65%), Postives = 56/104 (53.85%), Query Frame = 0


Query: 10  AAIVVSLLLVV------NHSSSLVAAN-LITETCSKTSHVDLCISSLGVDPRSPTADVRR 69
           +++ VSLLLV+      +   S+ A N LI+E C KT +  LC+ +L  DPRS + D++ 
Sbjct: 8   SSLFVSLLLVILFISPLSQRPSVKAENHLISEICPKTRNPSLCLQALESDPRSASKDLKG 67

Query: 70  LAEIILKETLANAQEILAHRVESFINQTTDPAVKECLAACKVLY 107
           L +  +    A+A++  +  + S  NQ TDP +K     C   Y
Sbjct: 68  LGQFSIDIAQASAKQ-TSKIIASLTNQATDPKLKGRYETCSENY 110

[Close]

BLAST of MS012090.1 vs. ExPASy TrEMBL
Match: A0A7N2LXR4 (PMEI domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 5.1e-10
Identity = 42/95 (44.21%), Postives = 58/95 (61.05%), Query Frame = 0


Query: 28  AANLITETCSKTSHVDLCISSLGVDPRSPTADVRRLAEIILKETLANAQEILAHRVESFI 87
           + NLI  TC KTSH DLC+SSL  +P+S TADV+ LA+I+    LAN  + L + +E  I
Sbjct: 34  SGNLIEHTCKKTSHYDLCLSSLQSNPQSATADVKGLAQIMADILLANVTDTLNY-IEGLI 93

Query: 88  NQTTDPAVKECLAACKVLYRLVTNSTIPSAMWQLS 123
            Q+ +P ++  L  C  LY  V   T+P A+  LS
Sbjct: 94  KQSPEPELERSLTYCAELYIPVVKYTLPQAIDALS 127

[Close]

BLAST of MS012090.1 vs. ExPASy TrEMBL
Match: M5XUJ1 (PMEI domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_3G007700 PE=4 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 6.6e-10
Identity = 44/102 (43.14%), Postives = 63/102 (61.76%), Query Frame = 0


Query: 7   IKWAAIVVSLLLVVNHSSSLVAANLITETCSKTSHVDLCISSLGVDPRSPTADVRRLAEI 66
           +KW  I    L V++ S   V+ +L+ ETC KT++ DLC+SSL  DPRS TADV+ LA I
Sbjct: 4   LKW-IIACLTLFVLSQSRGSVSTDLVEETCHKTTNYDLCVSSLKSDPRSSTADVKGLARI 63

Query: 67  ILKETLANAQEILAHRVESFINQTTDPAVKECLAACKVLYRL 109
            L +TL N+ +  A R+    N+T+D  +++ L  CK  Y L
Sbjct: 64  ALDQTLTNSVDTQA-RIARLFNETSDEYIRKGLGTCKDEYDL 103

[Close]

BLAST of MS012090.1 vs. ExPASy TrEMBL
Match: A0A5N5L7V8 (PMEI domain-containing protein OS=Salix brachista OX=2182728 GN=DKX38_016388 PE=4 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 6.6e-10
Identity = 44/117 (37.61%), Postives = 72/117 (61.54%), Query Frame = 0


Query: 7   IKWAAIVVSLLLV--VNHSSSLVAANLITETCSKTSHVDLCISSLGVDPRSPTADVRRLA 66
           +K+ A +   LLV  ++  ++LV A+L+ ETC KT + DLC+ +L  +PRS TADV+ L 
Sbjct: 1   MKFLACLPMFLLVNFLDQPTTLVGADLVQETCQKTRYPDLCVKTLKSNPRSSTADVKGLV 60

Query: 67  EIILKETLANAQEILAHRVESFINQTTDPAVKECLAACKVLYRLVTNSTIPSAMWQL 122
            I+L+  LAN++  LA +V+  + ++ D ++K+CL  C   Y    N   P+A+  L
Sbjct: 61  HIMLEANLANSKVTLA-KVDKLLKESGDKSLKKCLDDCAEEYDTAANEDFPTAIQSL 116

[Close]

BLAST of MS012090.1 vs. ExPASy TrEMBL
Match: A0A5E4F258 (PREDICTED: cell wall OS=Prunus dulcis OX=3755 GN=ALMOND_2B015884 PE=4 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 1.5e-09
Identity = 44/102 (43.14%), Postives = 62/102 (60.78%), Query Frame = 0


Query: 7   IKWAAIVVSLLLVVNHSSSLVAANLITETCSKTSHVDLCISSLGVDPRSPTADVRRLAEI 66
           +KW  I    L V++ S   V+ + I ETC KT++ DLC+SSL  DPRS TADV+ LA I
Sbjct: 4   LKW-IIACLTLFVLSQSRGSVSTDFIQETCHKTTNYDLCVSSLKSDPRSSTADVKGLARI 63

Query: 67  ILKETLANAQEILAHRVESFINQTTDPAVKECLAACKVLYRL 109
            L +TL N+ +  A R+    N+T+D  +++ L  CK  Y L
Sbjct: 64  ALDQTLTNSVDTQA-RIARLFNETSDEYIRKGLGTCKDEYDL 103

[Close]

BLAST of MS012090.1 vs. ExPASy TrEMBL
Match: A0A061DR99 (PMEI domain-containing protein OS=Theobroma cacao OX=3641 GN=TCM_004852 PE=4 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 2.5e-09
Identity = 44/115 (38.26%), Postives = 75/115 (65.22%), Query Frame = 0


Query: 7   IKWAAIVVSLLLVVNHSSSLVAANLITETCSKTSHVDLCISSLGVDPRSPTADVRRLAEI 66
           +K A +V+ ++L++   S  V A++I +TC++TS  +LC+S+L  DPRS TAD + LA I
Sbjct: 4   LKVADLVLLIVLLLFLMS--VRADMIDDTCNRTSDTELCLSTLRSDPRSSTADAKGLAHI 63

Query: 67  ILKETLANAQEILAHRVESFINQTTDPAVKECLAACKVLYRLVTNSTIPSAMWQL 122
           +L  TL  A++ +A +++  +++TTDPA+K+ L  C   Y   T   +P A+ +L
Sbjct: 64  VLDITLKKAKDNVA-QIDQLLSRTTDPALKDVLTICSQEYGNATVVILPRAIQKL 115

[Close]

BLAST of MS012090.1 vs. TAIR 10
Match: AT5G64620.1 (cell wall / vacuolar inhibitor of fructosidase 2 )

HSP 1 Score: 48.5 bits (114), Expect = 4.4e-06
Identity = 35/110 (31.82%), Postives = 56/110 (50.91%), Query Frame = 0


Query: 10  AAIVVSLLLV--VNHSSSLVAA-----NLITETCSKTSHVDLCISSLGVDPRSPTADVRR 69
           A+ ++ LLLV     +S+L++A      +I  TC  T++   C+S+L  DPRSPTAD + 
Sbjct: 2   ASSLIFLLLVTLTFSASTLISAKSNTTTIIESTCKTTNYYKFCVSALKSDPRSPTADTKG 61

Query: 70  LAEIILKETLANAQEILAHRVESFINQTTDPAVKECLAACKVLYRLVTNS 113
           LA I++   + NA     +   +      D  +K+ L  C   Y L  +S
Sbjct: 62  LASIMVGVGMTNATSTANYIAGNLSATVKDTVLKKVLQDCSEKYALAADS 111

[Close]

BLAST of MS012090.1 vs. TAIR 10
Match: AT1G47960.1 (cell wall / vacuolar inhibitor of fructosidase 1 )

HSP 1 Score: 43.1 bits (100), Expect = 1.8e-04
Identity = 31/122 (25.41%), Postives = 61/122 (50.00%), Query Frame = 0


Query: 1   LKMFPLIKWAAIVVSLLLVVNHSSSLVAANLITETCSKTSHVDLCISSLGVDPRSPTADV 60
           +KM  ++    +++ ++++V+  S      +I  TC +T   +LC+S L  DPR  +AD 
Sbjct: 1   MKMMKVMMLIVMMMMVMVMVSEGS------IIEPTCKETPDFNLCVSLLNSDPRGSSADT 60

Query: 61  RRLAEIILKETLANAQEILAHRVESFINQTTDPAVKECLAACKVLYRLVTNSTIPSAMWQ 120
             LA I++ +    A + L + +     +   P +K  L  C   Y+ + N+ +P A+  
Sbjct: 61  SGLALILIDKIKGLATKTL-NEINGLYKKR--PELKRALDECSRRYKTILNADVPEAIEA 113

Query: 121 LS 123
           +S
Sbjct: 121 IS 113

The following BLAST results are available for this feature:

BLAST of MS012090.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023890881.1	4.7e-10	45.26	cell wall / vacuolar inhibitor of fructosidase 1-like [Quercus suber]	[more]
XP_030975931.1	1.0e-09	44.21	cell wall / vacuolar inhibitor of fructosidase 1-like [Quercus lobata]	[more]
XP_017969808.1	1.0e-09	40.00	PREDICTED: pectinesterase inhibitor [Theobroma cacao]	[more]
KAG5237625.1	1.4e-09	38.46	pectinesterase inhibitor [Salix suchowensis]	[more]
XP_007221576.1	1.4e-09	43.14	cell wall / vacuolar inhibitor of fructosidase 2 [Prunus persica] >ONI14778.1 hy...	[more]

BLAST of MS012090.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49603	6.2e-05	31.82	Cell wall / vacuolar inhibitor of fructosidase 2 OS=Arabidopsis thaliana OX=3702...	[more]
Q0J8J8	1.4e-04	36.78	Pectinesterase inhibitor 28 OS=Oryza sativa subsp. japonica OX=39947 GN=PMEI28 P...	[more]
P83326	3.1e-04	33.65	Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2	[more]

BLAST of MS012090.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A7N2LXR4	5.1e-10	44.21	PMEI domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1	[more]
M5XUJ1	6.6e-10	43.14	PMEI domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_3G007700 PE=4 ...	[more]
A0A5N5L7V8	6.6e-10	37.61	PMEI domain-containing protein OS=Salix brachista OX=2182728 GN=DKX38_016388 PE=...	[more]
A0A5E4F258	1.5e-09	43.14	PREDICTED: cell wall OS=Prunus dulcis OX=3755 GN=ALMOND_2B015884 PE=4 SV=1	[more]
A0A061DR99	2.5e-09	38.26	PMEI domain-containing protein OS=Theobroma cacao OX=3641 GN=TCM_004852 PE=4 SV=...	[more]

BLAST of MS012090.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G64620.1	4.4e-06	31.82	cell wall / vacuolar inhibitor of fructosidase 2	[more]
AT1G47960.1	1.8e-04	25.41	cell wall / vacuolar inhibitor of fructosidase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 27..122 e-value: 4.9E-20 score: 74.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 30..120
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 12..112 e-value: 1.5E-21 score: 74.5
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 30..110 e-value: 9.1E-13 score: 48.8
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 13..107
None	No IPR available	PANTHER	PTHR31080:SF152	CELL WALL / VACUOLAR INHIBITOR OF FRUCTOSIDASE 2	coord: 13..107

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS012090	MS012090	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS012090.1-cds	MS012090.1-cds-scaffold708:211803..212168	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS012090.1	MS012090.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004857	enzyme inhibitor activity