Homology
BLAST of MS009042 vs. NCBI nr
Match:
XP_022146462.1 (probable RNA-dependent RNA polymerase 1 [Momordica charantia])
HSP 1 Score: 2187.5 bits (5667), Expect = 0.0e+00
Identity = 1083/1103 (98.19%), Postives = 1088/1103 (98.64%), Query Frame = 0
Query: 1 MGKTIEIYGFRRDVTEEEVKEFLENHTGDGTVSEVRIAESRDENSRFKFATVRFTSELAA 60
MGKTIEIYGFRRDVT EEVKEFLENHTGDGTVS VRIAE RDENSRF FATV+F+SELAA
Sbjct: 1 MGKTIEIYGFRRDVTAEEVKEFLENHTGDGTVSAVRIAEFRDENSRFTFATVQFSSELAA 60
Query: 61 GHVVSLAAAEERLWYGSSYLKAREADRQNRRGGVGVERMENLTLHLGSQISKEKLSVIWK 120
GHVVS AAAEERLWYGSSYLKAREADRQNRRGGVGVERMENLTLHLGSQISKEKLSVIWK
Sbjct: 61 GHVVSQAAAEERLWYGSSYLKAREADRQNRRGGVGVERMENLTLHLGSQISKEKLSVIWK 120
Query: 121 GENCSVEFGSGVRKLFVYVNLEYKMEVWFESILRVQLRCPPDGASKFLLIQLQGAPRIFK 180
GENCSVEFGSGVRKLFVYVNLEYKMEVWFESILRVQLRCPPDGASKFLLIQLQ APRIFK
Sbjct: 121 GENCSVEFGSGVRKLFVYVNLEYKMEVWFESILRVQLRCPPDGASKFLLIQLQRAPRIFK 180
Query: 181 KRSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPGDQLPSF 240
K SSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPGDQLPSF
Sbjct: 181 KSSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPGDQLPSF 240
Query: 241 FKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGFDLSYKILFKINALVQHGYLPGP 300
FKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGFD S+KILFKINALVQHGYLPGP
Sbjct: 241 FKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGFDFSFKILFKINALVQHGYLPGP 300
Query: 301 LLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQNWLKQQYRSYYNSKQLPWKPDI 360
LLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQ WLKQQY SYYNSKQLPWKPDI
Sbjct: 301 LLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQKWLKQQYLSYYNSKQLPWKPDI 360
Query: 361 SLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELDKIRPID 420
SLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELDKIR ID
Sbjct: 361 SLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELDKIRSID 420
Query: 421 LSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFASREGLSA 480
LSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFASREGLSA
Sbjct: 421 LSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFASREGLSA 480
Query: 481 ADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETNNVTYCFSD 540
ADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETNNVTYCFSD
Sbjct: 481 ADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETNNVTYCFSD 540
Query: 541 GIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPKSEKKLSLRKSMLKYKSL 600
GIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDP SEKKLSLRKSMLKYKSL
Sbjct: 541 GIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPTSEKKLSLRKSMLKYKSL 600
Query: 601 DTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLDCILKDPSRALEVL 660
DTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQL+CILKDPSRALEVL
Sbjct: 601 DTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLNCILKDPSRALEVL 660
Query: 661 ELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCL 720
ELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCL
Sbjct: 661 ELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCL 720
Query: 721 DETGTLKYGQVFVQCSVPGRSSESNFIAEGKVVVAKNPCLHPGDMRVLNAVDVKALHHMV 780
DETGTLKYGQVFVQCSVPGRSSESNFI EGKVVVAKNPCLHPGDMRVLNAVDVKALHHMV
Sbjct: 721 DETGTLKYGQVFVQCSVPGRSSESNFIPEGKVVVAKNPCLHPGDMRVLNAVDVKALHHMV 780
Query: 781 DCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELTCIRPVKPMSYKPAPTIQLDHDVTI 840
DCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPEL CIRPVKPMSYKPAPTIQLDHDVTI
Sbjct: 781 DCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELNCIRPVKPMSYKPAPTIQLDHDVTI 840
Query: 841 EEVQEYMANYMVNDGLGAIANAHTAFADREPRKAMSAKCIKLAKLFSIAVDFPKTGVPAN 900
EEVQEYMANYMVNDGLGAIANAHT FADREPRKAMSAKCIKLAKLFSIAVDFPKTGVPAN
Sbjct: 841 EEVQEYMANYMVNDGLGAIANAHTVFADREPRKAMSAKCIKLAKLFSIAVDFPKTGVPAN 900
Query: 901 LPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREVAAKCYDPD 960
LPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREVAAKCYDPD
Sbjct: 901 LPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREVAAKCYDPD 960
Query: 961 MEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFDKRTDMEQI 1020
MEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFDKRTDMEQI
Sbjct: 961 MEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFDKRTDMEQI 1020
Query: 1021 SHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEEEEFAKASAWYHVTYHQSYWGRYNEGME 1080
SHAMRSLRKEARAWFNEKGSKPRCDDDDNGDE+EEFAKASAWYHVTYHQSYWGRYNEGME
Sbjct: 1021 SHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEDEEFAKASAWYHVTYHQSYWGRYNEGME 1080
Query: 1081 RDHFLSFPWCVSDKLIQIKKKKI 1104
RDHFLSFPWCVSDKLIQIKKKKI
Sbjct: 1081 RDHFLSFPWCVSDKLIQIKKKKI 1103
BLAST of MS009042 vs. NCBI nr
Match:
XP_038875555.1 (probable RNA-dependent RNA polymerase 1 [Benincasa hispida])
HSP 1 Score: 1788.1 bits (4630), Expect = 0.0e+00
Identity = 883/1107 (79.77%), Postives = 982/1107 (88.71%), Query Frame = 0
Query: 1 MGKTIEIYGFRRDVTEEEVKEFLENHTGDGTVSEVRIAESRDENSRFKFATVRFTSELAA 60
MGKTIEIYGFR +VT +EVKEFLENHTGDGTV VRI++ +DE +RF F TVRF SELAA
Sbjct: 1 MGKTIEIYGFRPEVTVDEVKEFLENHTGDGTVLTVRISKPKDEKTRFTFVTVRFKSELAA 60
Query: 61 GHVVSLA-AAEERLWYGSSYLKAREADRQN-RRGGVGVERMENLTLHLGSQISKEKLSVI 120
++V A AAE++LW+GSSYLKARE +R + RGG +ERME++ HLGS ISK+K+ VI
Sbjct: 61 EYIVEKATAAEKKLWFGSSYLKAREVERASPARGGGEMERMEDVKGHLGSLISKDKMRVI 120
Query: 121 WKGENCSVEFGSGVRKLFVYVN--LEYKMEVWFESILRVQLRCPPDGASKFLLIQLQGAP 180
W+GEN +VEFG GVRKL Y++ +EYKME+ FE+IL V+ RCP + SKF LIQLQGAP
Sbjct: 121 WEGENWNVEFGIGVRKLSFYLSYEVEYKMELAFENILGVEFRCPLNQPSKFFLIQLQGAP 180
Query: 181 RIFKKRSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPGDQ 240
RIFKK S SSSSS +S+ F F RWIRDVDFTPSSCIGQSFALCLELSPG
Sbjct: 181 RIFKK--SRSSSSSRLNSNEFCF--------RWIRDVDFTPSSCIGQSFALCLELSPGHH 240
Query: 241 LPSFFKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGFDLSYKILFKINALVQHGY 300
LP FF+ LVGY+E Y PF L+TGSSFSS SNLVPI+TPP+GFD+SYK LFKINALVQHGY
Sbjct: 241 LPPFFQNLVGYKETYAPFILQTGSSFSSISNLVPIITPPRGFDISYKTLFKINALVQHGY 300
Query: 301 LPGPLLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQNWLKQQYRSYYNSKQLPW 360
LPGP LDDEFFRLVD SRFRP+Y+E ALEK+FNLKEC YEPQ WLK Y S+Y S QLPW
Sbjct: 301 LPGPTLDDEFFRLVDSSRFRPDYVEHALEKMFNLKECCYEPQKWLKHHYLSFYTSNQLPW 360
Query: 361 KPDISLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELDKI 420
KP+ISLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVR++ DDIDNF+RVSFVDE+LDK+
Sbjct: 361 KPNISLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEDLDKL 420
Query: 421 RPIDLSPRTSTA-NGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFASR 480
IDL+PR+S+A N ERTRVYDRILS LRNGIVIGDKKFEFLAFSASQLRENS WMFASR
Sbjct: 421 HSIDLAPRSSSAGNSERTRVYDRILSVLRNGIVIGDKKFEFLAFSASQLRENSFWMFASR 480
Query: 481 EGLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETNNVT 540
EGLSAADIREWMGDFR+IRNVAKYAARLGQSFGSSRKTLCVEEHE EVI DVEVE
Sbjct: 481 EGLSAADIREWMGDFREIRNVAKYAARLGQSFGSSRKTLCVEEHEFEVIPDVEVERKKTK 540
Query: 541 YCFSDGIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPKSEKKLSLRKSML 600
YCFSDGIGKIS+TLAEKVAE+CGLI+HTPSAFQIRYAGYKGVVAIDP +KKLSLRKSML
Sbjct: 541 YCFSDGIGKISKTLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTLKKKLSLRKSML 600
Query: 601 KYKSLDTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLDCILKDPSR 660
KY SLDTQLDVL WSKY PCFLNRQVINLLSTLGIKDRVF KKQKEAI QLD IL+DPS+
Sbjct: 601 KYMSLDTQLDVLLWSKYQPCFLNRQVINLLSTLGIKDRVFVKKQKEAIDQLDSILEDPSK 660
Query: 661 ALEVLELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRT 720
ALEVLELMSPGEMT +LKE+LL+YKPN+EPFLNMMLRTFRADK LDLRTKSRIFVPKGRT
Sbjct: 661 ALEVLELMSPGEMTGILKELLLFYKPNEEPFLNMMLRTFRADKFLDLRTKSRIFVPKGRT 720
Query: 721 MMGCLDETGTLKYGQVFVQCSVPGRSSESNFIAEGKVVVAKNPCLHPGDMRVLNAVDVKA 780
MMGCLDET TL+YGQVFV CS+PGRS+ESNF+ +GKVVVAKNPCLHPGD+RVL+AVDVKA
Sbjct: 721 MMGCLDETRTLEYGQVFVHCSIPGRSNESNFVIKGKVVVAKNPCLHPGDVRVLDAVDVKA 780
Query: 781 LHHMVDCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELTCIRPVKPMSYKPAPTIQLD 840
LHHMVDCVVFPQKG+RPHPNECSGSDLDGDLYFVCWDPELTCI+PVKPMSY+PAPT+QLD
Sbjct: 781 LHHMVDCVVFPQKGKRPHPNECSGSDLDGDLYFVCWDPELTCIKPVKPMSYEPAPTMQLD 840
Query: 841 HDVTIEEVQEYMANYMVNDGLGAIANAHTAFADREPRKAMSAKCIKLAKLFSIAVDFPKT 900
HDVTIEEVQEY+ANYMVNDGLGAIANAHT FAD+ P+KAMS +CIKLAKLFSIAVDFPKT
Sbjct: 841 HDVTIEEVQEYLANYMVNDGLGAIANAHTVFADKNPKKAMSTECIKLAKLFSIAVDFPKT 900
Query: 901 GVPANLPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREVAAK 960
GVPANLP LRVHE+PDFM+K ++P Y S GV+GKLF+ VKDVS + NT+E +TREVA
Sbjct: 901 GVPANLPRNLRVHEYPDFMDKPNKPTYASNGVLGKLFQGVKDVSSDVNTLEIFTREVAIN 960
Query: 961 CYDPDMEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFDKRT 1020
CYDPDME+DGF+KYL+EAFD+KTRYD+KLGNLMDYYGI+TEPELVSGNILR+ KSFDKR
Sbjct: 961 CYDPDMEVDGFDKYLSEAFDYKTRYDFKLGNLMDYYGIKTEPELVSGNILRMAKSFDKRN 1020
Query: 1021 DMEQISHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEEEEFAKASAWYHVTYHQSYWGRY 1080
D+EQI+ AM+SLRKEARAWFNEKGSK D+ EEEE+AKASAWYHVTYH YWGRY
Sbjct: 1021 DLEQITFAMKSLRKEARAWFNEKGSKSTYDNSKG--EEEEYAKASAWYHVTYHPDYWGRY 1080
Query: 1081 NEGMERDHFLSFPWCVSDKLIQIKKKK 1103
NEGM+RDHFLSFPWCV+DKLIQIK++K
Sbjct: 1081 NEGMQRDHFLSFPWCVADKLIQIKREK 1095
BLAST of MS009042 vs. NCBI nr
Match:
KAG6579193.1 (RNA-dependent RNA polymerase 1, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1770.0 bits (4583), Expect = 0.0e+00
Identity = 874/1110 (78.74%), Postives = 975/1110 (87.84%), Query Frame = 0
Query: 1 MGKTIEIYGFRRDVTEEEVKEFLENHTGDGTVSEVRIAESRDENSRFKFATVRFTSELAA 60
MGKTIEIYGF +VT +EVKEF+ENHTGDGTV VRI++ DE +RF FATVRFTS+L
Sbjct: 7 MGKTIEIYGFPAEVTADEVKEFIENHTGDGTVLTVRISKFNDEKARFTFATVRFTSKLGG 66
Query: 61 GHVVSLAAAEERLWYGSSYLKAREADRQNRRGGVG-----VERMENLTLHLGSQISKEKL 120
+VV+ AAAE+RLW+GSSYLKAR+ +R+ + +ERMEN+ + GS +SKEK+
Sbjct: 67 EYVVAQAAAEKRLWFGSSYLKARKVEREIKTAAAARVHGELERMENVNVQWGSLVSKEKM 126
Query: 121 SVIWKGENCSVEFGSGVRKLFVYVN---LEYKMEVWFESILRVQLRCPPDGASKFLLIQL 180
VIWKG SVE+G GVRKL Y++ +EYKME+ FE+IL VQLRC D SK LIQL
Sbjct: 127 KVIWKGGKWSVEYGIGVRKLGFYLSYEGVEYKMELCFENILGVQLRCSVDRGSKLFLIQL 186
Query: 181 QGAPRIFKKRSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELS 240
QGAPRIFKK SSSS SS +S+G RWIRDVDFTPSSCIGQSFALCLELS
Sbjct: 187 QGAPRIFKKAPLSSSSRLSS---------EESTGFRWIRDVDFTPSSCIGQSFALCLELS 246
Query: 241 PGDQLPSFFKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGFDLSYKILFKINALV 300
GDQLPSFF+TLVGY+E Y PF L+TGSS SS SNLVPI+TPPQGFD+ YKILFKINAL+
Sbjct: 247 HGDQLPSFFQTLVGYKESYAPFILQTGSSLSSISNLVPIITPPQGFDIPYKILFKINALL 306
Query: 301 QHGYLPGPLLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQNWLKQQYRSYYNSK 360
QHGYLPGP LD+EFFRLVD SRFRP+Y+E AL+KLF+LKEC YEPQ WLKQQY S+Y+SK
Sbjct: 307 QHGYLPGPALDNEFFRLVDSSRFRPDYVEYALDKLFHLKECCYEPQKWLKQQYLSFYSSK 366
Query: 361 QLPWKPDISLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEE 420
QLPWKP+ISLDDGLVYVHRVQITP+KVYFRGPEANLSNRVVR + DD+DNF+RVSFVDEE
Sbjct: 367 QLPWKPNISLDDGLVYVHRVQITPTKVYFRGPEANLSNRVVRHFIDDVDNFLRVSFVDEE 426
Query: 421 LDKIRPIDLSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMF 480
LDK+ IDLSPRTS N RTRVYDR+LS LRNGIVIGDKKFEFLAFSASQLRENS WMF
Sbjct: 427 LDKLHSIDLSPRTSAENDLRTRVYDRVLSVLRNGIVIGDKKFEFLAFSASQLRENSCWMF 486
Query: 481 ASREGLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETN 540
ASR+GLSAADIREWMGD RQIRNVAKYAARLGQSFGSSR+TLCVE+HEIEVI+DVEVETN
Sbjct: 487 ASRKGLSAADIREWMGDLRQIRNVAKYAARLGQSFGSSRRTLCVEQHEIEVISDVEVETN 546
Query: 541 NVTYCFSDGIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPKSEKKLSLRK 600
+TYCFSDGIGKIS TLAEKVAE+CGLI+HTPSAFQIRYAGYKGVVAIDP S KKLSLRK
Sbjct: 547 KITYCFSDGIGKISGTLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSTKKLSLRK 606
Query: 601 SMLKYKSLDTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLDCILKD 660
SMLKY SLDTQ+DVL+WSKY PCFLNRQVINLLSTLGIKDRVF KKQKEAI QLD IL+D
Sbjct: 607 SMLKYTSLDTQVDVLSWSKYQPCFLNRQVINLLSTLGIKDRVFVKKQKEAIDQLDSILED 666
Query: 661 PSRALEVLELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPK 720
PSRALEVLELMSPGEMTS+LKE+LL YKPN+EPFLNMMLRTFRA KLLDLRTKSRIFVPK
Sbjct: 667 PSRALEVLELMSPGEMTSILKELLLLYKPNEEPFLNMMLRTFRAAKLLDLRTKSRIFVPK 726
Query: 721 GRTMMGCLDETGTLKYGQVFVQCSVPGRSSESNFIAEGKVVVAKNPCLHPGDMRVLNAVD 780
GRTMMGCLDET TL+YGQVFV CS+PGRSSESNF+ +GKVVVAKNPCLHPGD+R+L+AVD
Sbjct: 727 GRTMMGCLDETRTLEYGQVFVHCSLPGRSSESNFVVKGKVVVAKNPCLHPGDVRLLDAVD 786
Query: 781 VKALHHMVDCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELTCIRPVKPMSYKPAPTI 840
VKALHHMVDCVVFPQKG+RPHPNECSGSDLDGDLYFV WDPELT I+PVKPMSY+PAPT+
Sbjct: 787 VKALHHMVDCVVFPQKGERPHPNECSGSDLDGDLYFVGWDPELTRIKPVKPMSYEPAPTM 846
Query: 841 QLDHDVTIEEVQEYMANYMVNDGLGAIANAHTAFADREPRKAMSAKCIKLAKLFSIAVDF 900
QLDHDVTIEEVQ Y+ YMVNDGLG IANAHT FAD++P+KAM+A+CIKLAKLFSIAVDF
Sbjct: 847 QLDHDVTIEEVQGYLVKYMVNDGLGGIANAHTVFADKKPKKAMTAECIKLAKLFSIAVDF 906
Query: 901 PKTGVPANLPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREV 960
PKTGVPAN P LRV E+PDFMEK D+P Y S GV+GKLFR VKDVS + NT+E ++REV
Sbjct: 907 PKTGVPANFPHNLRVREYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDVNTVEIFSREV 966
Query: 961 AAKCYDPDMEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFD 1020
A KCYDPDME+DGFE YL+EAF+FK RYD+KLGNLMDYYGI+TEPEL+SGNILR+ KSFD
Sbjct: 967 ATKCYDPDMEVDGFEDYLSEAFEFKARYDFKLGNLMDYYGIKTEPELISGNILRMAKSFD 1026
Query: 1021 KRTDMEQISHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEEEEFAKASAWYHVTYHQSYW 1080
KR DMEQIS AM+SLRKEAR+WFNEKGSK D+DDN + EE+AKASAWY VTYH YW
Sbjct: 1027 KRNDMEQISLAMKSLRKEARSWFNEKGSKSAYDEDDN--KNEEYAKASAWYRVTYHPDYW 1086
Query: 1081 GRYNEGMERDHFLSFPWCVSDKLIQIKKKK 1103
GRYNEGM+RDHFLSFPWCVSDKLI+IK++K
Sbjct: 1087 GRYNEGMQRDHFLSFPWCVSDKLIRIKREK 1105
BLAST of MS009042 vs. NCBI nr
Match:
XP_022994004.1 (probable RNA-dependent RNA polymerase 1 [Cucurbita maxima])
HSP 1 Score: 1767.3 bits (4576), Expect = 0.0e+00
Identity = 875/1113 (78.62%), Postives = 976/1113 (87.69%), Query Frame = 0
Query: 1 MGKTIEIYGFRRDVTEEEVKEFLENHTGDGTVSEVRIAESRDENSRFKFATVRFTSELAA 60
MGK IEIYGF +VT +EVKEF+ENHTGDGTV VRI++ DE +RF FATVRFTS+L
Sbjct: 7 MGKMIEIYGFPAEVTADEVKEFIENHTGDGTVLTVRISKPNDEKARFTFATVRFTSKLGG 66
Query: 61 GHVVSLAAAEERLWYGSSYLKAREADRQNR--------RGGVGVERMENLTLHLGSQISK 120
+VV+ AAAE+RLW+GSSYLKAR+ +R+ R R G+ERMEN+ + GS +SK
Sbjct: 67 EYVVAQAAAEKRLWFGSSYLKARKVEREIRTATAAAAARVHGGLERMENVNVQWGSLVSK 126
Query: 121 EKLSVIWKGENCSVEFGSGVRKLFVYVN---LEYKMEVWFESILRVQLRCPPDGASKFLL 180
EK+ VIWK SVE+G G RKL Y++ +EYKME+ FE+IL VQLRC D S+ L
Sbjct: 127 EKMKVIWKEGKWSVEYGIGARKLGFYLSYEGVEYKMELCFENILGVQLRCSGDRGSELFL 186
Query: 181 IQLQGAPRIFKKRSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCL 240
IQLQGAPRIFKK SSSSS SS +S+G RWIRDVDFTPSSCIGQSFALCL
Sbjct: 187 IQLQGAPRIFKKTPSSSSSRLSS---------EESTGFRWIRDVDFTPSSCIGQSFALCL 246
Query: 241 ELSPGDQLPSFFKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGFDLSYKILFKIN 300
ELSPGDQLPSFF+TLVGY+E Y PF L GSS SS SNLVPI+TPPQGFD+SYKILFKIN
Sbjct: 247 ELSPGDQLPSFFRTLVGYKESYAPFILHPGSSLSSISNLVPIITPPQGFDISYKILFKIN 306
Query: 301 ALVQHGYLPGPLLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQNWLKQQYRSYY 360
AL+QHGYLPGP LD+EFFRLVD SRFRPEYIE ALEKLF+ KEC YEPQ WLKQQY S+Y
Sbjct: 307 ALLQHGYLPGPALDNEFFRLVDSSRFRPEYIEYALEKLFHFKECCYEPQKWLKQQYLSFY 366
Query: 361 NSKQLPWKPDISLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFV 420
+SKQLP KP++SLDDGLVYVHRVQITP+KVYFRGPEANLSNRVVR + DD+DNF+RVSFV
Sbjct: 367 SSKQLPCKPNVSLDDGLVYVHRVQITPTKVYFRGPEANLSNRVVRHFIDDVDNFLRVSFV 426
Query: 421 DEELDKIRPIDLSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSV 480
DEELDK+ IDLSPRTS N RTRVYDR+LS LRNGIVIGDKKFEFLAFSASQLRENS
Sbjct: 427 DEELDKLHSIDLSPRTSAENDLRTRVYDRVLSVLRNGIVIGDKKFEFLAFSASQLRENSC 486
Query: 481 WMFASREGLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEV 540
WMFASR+GLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSR+TLCVE+HEIEVI DVEV
Sbjct: 487 WMFASRKGLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRRTLCVEQHEIEVIPDVEV 546
Query: 541 ETNNVTYCFSDGIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPKSEKKLS 600
ETN +TYCFSDGIGKIS TLAEKVAE+CGLI+HTPSAFQIRYAGYKGVVAIDP S KKLS
Sbjct: 547 ETNKITYCFSDGIGKISGTLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSTKKLS 606
Query: 601 LRKSMLKYKSLDTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLDCI 660
LRKSMLKY SLDTQ+DVL+WSKY PCFLNRQVINLLSTLGIKDRVF KKQKEAI QLD I
Sbjct: 607 LRKSMLKYTSLDTQVDVLSWSKYQPCFLNRQVINLLSTLGIKDRVFVKKQKEAIDQLDSI 666
Query: 661 LKDPSRALEVLELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIF 720
L+DPSRALEVLELMSPGEMTS+LKE+LL YKPN+EPFLNMMLRTFRA KLLDLRTKSRIF
Sbjct: 667 LEDPSRALEVLELMSPGEMTSILKELLLLYKPNEEPFLNMMLRTFRAAKLLDLRTKSRIF 726
Query: 721 VPKGRTMMGCLDETGTLKYGQVFVQCSVPGRSSESNFIAEGKVVVAKNPCLHPGDMRVLN 780
VPKGRTMMGCLDET TL+YGQVFV CS+PGRSSESNF+ +GKVVVAKNPCLHPGD+R+L+
Sbjct: 727 VPKGRTMMGCLDETRTLEYGQVFVHCSLPGRSSESNFVVKGKVVVAKNPCLHPGDVRLLD 786
Query: 781 AVDVKALHHMVDCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELTCIRPVKPMSYKPA 840
AVDVKALHHMV+CVVFPQKG+RPHPNECSGSDLDGDLYFV WDPELT I+PVKPMSY+PA
Sbjct: 787 AVDVKALHHMVNCVVFPQKGERPHPNECSGSDLDGDLYFVGWDPELTRIKPVKPMSYEPA 846
Query: 841 PTIQLDHDVTIEEVQEYMANYMVNDGLGAIANAHTAFADREPRKAMSAKCIKLAKLFSIA 900
PT++LDHDVTIEEVQEY+A YMVNDGLG IANAHT FAD++P+KAM+A+CIKLAKLFSIA
Sbjct: 847 PTMRLDHDVTIEEVQEYLAKYMVNDGLGGIANAHTVFADKKPKKAMTAECIKLAKLFSIA 906
Query: 901 VDFPKTGVPANLPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYT 960
VDFPKTGVPAN P LRV E+PDFMEK D+P Y S GV+GKLFR VKDVS + NTIE ++
Sbjct: 907 VDFPKTGVPANFPHNLRVREYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDVNTIEIFS 966
Query: 961 REVAAKCYDPDMEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGK 1020
REVA KCYDPDME+DGFE YL+EAF++K RYD+KLGNLMDYYGI+TEPEL+SGNILR+ K
Sbjct: 967 REVATKCYDPDMEVDGFEDYLSEAFEYKARYDFKLGNLMDYYGIKTEPELISGNILRMAK 1026
Query: 1021 SFDKRTDMEQISHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEEEEFAKASAWYHVTYHQ 1080
SFDKR D+EQIS A++SLRKEAR+WFNEKGSK CD+D D++EE+AKASAWY VTYH
Sbjct: 1027 SFDKRNDLEQISLAIKSLRKEARSWFNEKGSKSACDED---DKDEEYAKASAWYRVTYHP 1086
Query: 1081 SYWGRYNEGMERDHFLSFPWCVSDKLIQIKKKK 1103
YWGRYNEGM+RDHFLSFPWCVSDKLIQIK++K
Sbjct: 1087 DYWGRYNEGMQRDHFLSFPWCVSDKLIQIKREK 1107
BLAST of MS009042 vs. NCBI nr
Match:
XP_022938807.1 (probable RNA-dependent RNA polymerase 1 [Cucurbita moschata])
HSP 1 Score: 1765.0 bits (4570), Expect = 0.0e+00
Identity = 873/1108 (78.79%), Postives = 974/1108 (87.91%), Query Frame = 0
Query: 1 MGKTIEIYGFRRDVTEEEVKEFLENHTGDGTVSEVRIAESRDENSRFKFATVRFTSELAA 60
MGKTIEIYGF VT +EVKEF+ENHTGDGTV VRI++ DE +RF FATV+FTS+L
Sbjct: 7 MGKTIEIYGFPTQVTADEVKEFIENHTGDGTVLTVRISKLNDEKARFTFATVQFTSKLGG 66
Query: 61 GHVVSLAAAEERLWYGSSYLKAREADRQNR---RGGVGVERMENLTLHLGSQISKEKLSV 120
+VV+ AAAE+RLW+GSSYLKAR+ +R+ + R +ERMEN+ + GS +SKE++ V
Sbjct: 67 EYVVAQAAAEKRLWFGSSYLKARKVEREIKTAARVHGELERMENVNVQWGSLVSKEEMKV 126
Query: 121 IWKGENCSVEFGSGVRKLFVYVN---LEYKMEVWFESILRVQLRCPPDGASKFLLIQLQG 180
IWKG SVE+G GVRKL Y++ +EYKME+ FE+IL VQLRC D SK LIQLQG
Sbjct: 127 IWKGGKWSVEYGIGVRKLGFYLSYEGVEYKMELCFENILGVQLRCSVDRGSKLFLIQLQG 186
Query: 181 APRIFKKRSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPG 240
APRIFKK SSSS SS +S+G RWIRDVDFTPSSCIGQSFALCLELS G
Sbjct: 187 APRIFKKAPLSSSSRLSS---------EESTGFRWIRDVDFTPSSCIGQSFALCLELSHG 246
Query: 241 DQLPSFFKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGFDLSYKILFKINALVQH 300
DQLPSFF+TLVGY+E Y PF L+TGSS SS SNLVPI+TPPQGFD+ YKILFKINAL+QH
Sbjct: 247 DQLPSFFQTLVGYKESYAPFILQTGSSLSSISNLVPIITPPQGFDIPYKILFKINALLQH 306
Query: 301 GYLPGPLLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQNWLKQQYRSYYNSKQL 360
GYLPGP LD+EFFRLVD SRFRP+Y+E AL+KLF+ KEC YEPQ WLKQQY S+Y+SKQL
Sbjct: 307 GYLPGPALDNEFFRLVDSSRFRPDYVEYALDKLFHFKECCYEPQKWLKQQYLSFYSSKQL 366
Query: 361 PWKPDISLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELD 420
PWKP+ISLDDGLVYVHRVQITP+KVYFRGPEANLSNRVVR + DD+DNF+RVSFVDEELD
Sbjct: 367 PWKPNISLDDGLVYVHRVQITPTKVYFRGPEANLSNRVVRHFIDDVDNFLRVSFVDEELD 426
Query: 421 KIRPIDLSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFAS 480
K+ IDLSPRTS N RTRVYDR+LS LRNGIVIGDKKFEFLAFSASQLRENS WMFAS
Sbjct: 427 KLHSIDLSPRTSAENDLRTRVYDRVLSVLRNGIVIGDKKFEFLAFSASQLRENSCWMFAS 486
Query: 481 REGLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETNNV 540
R+GLSAADIREWMGD RQIRNVAKYAARLGQSFGSSR+TLCVE+HEIEVI+DVEVETN +
Sbjct: 487 RKGLSAADIREWMGDLRQIRNVAKYAARLGQSFGSSRRTLCVEQHEIEVISDVEVETNKI 546
Query: 541 TYCFSDGIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPKSEKKLSLRKSM 600
TYCFSDGIGKIS TLAEKVAE+CGLI+HTPSAFQIRYAGYKGVVAIDP S KKLSLRKSM
Sbjct: 547 TYCFSDGIGKISGTLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSTKKLSLRKSM 606
Query: 601 LKYKSLDTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLDCILKDPS 660
LKY SLDTQ+DVL+WSKY PCFLNRQVINLLSTLGIKDRVF KKQKEAI QLD IL+DPS
Sbjct: 607 LKYTSLDTQVDVLSWSKYQPCFLNRQVINLLSTLGIKDRVFVKKQKEAIDQLDSILEDPS 666
Query: 661 RALEVLELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGR 720
RALEVLELMSPGEMTS+LKE+LL YKPN+EPFLNMMLRTFRA KLLDLRTKSRIFVPKGR
Sbjct: 667 RALEVLELMSPGEMTSILKELLLLYKPNEEPFLNMMLRTFRAAKLLDLRTKSRIFVPKGR 726
Query: 721 TMMGCLDETGTLKYGQVFVQCSVPGRSSESNFIAEGKVVVAKNPCLHPGDMRVLNAVDVK 780
TMMGCLDET TL+YGQVFV CS+PGRSSESNF+ +GKVVVAKNPCLHPGD+R+L+AVDVK
Sbjct: 727 TMMGCLDETRTLEYGQVFVHCSLPGRSSESNFVVKGKVVVAKNPCLHPGDVRLLDAVDVK 786
Query: 781 ALHHMVDCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELTCIRPVKPMSYKPAPTIQL 840
ALHHMVDCVVFPQKG+RPHPNECSGSDLDGDLYFV WDPELT I+PVKPMSY+PAPT+QL
Sbjct: 787 ALHHMVDCVVFPQKGKRPHPNECSGSDLDGDLYFVGWDPELTRIKPVKPMSYEPAPTMQL 846
Query: 841 DHDVTIEEVQEYMANYMVNDGLGAIANAHTAFADREPRKAMSAKCIKLAKLFSIAVDFPK 900
DHDVTIEEVQ Y+ YMVNDGLG IANAHT FAD++P+KAM+A+CIKLAKLFSIAVDFPK
Sbjct: 847 DHDVTIEEVQGYLVKYMVNDGLGGIANAHTVFADKKPKKAMAAECIKLAKLFSIAVDFPK 906
Query: 901 TGVPANLPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREVAA 960
TGVPAN P LRV E+PDFMEK D+P Y S GV+GKLFR VKDVS + NT+E ++REVA
Sbjct: 907 TGVPANFPHNLRVREYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDVNTVEIFSREVAT 966
Query: 961 KCYDPDMEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFDKR 1020
KCYDPDME+DGFE YL+EAF+FK RYD+KLGNLMDYYGI+TEPEL+SGNILR+ KSFDKR
Sbjct: 967 KCYDPDMEVDGFEDYLSEAFEFKARYDFKLGNLMDYYGIKTEPELISGNILRMAKSFDKR 1026
Query: 1021 TDMEQISHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEEEEFAKASAWYHVTYHQSYWGR 1080
DMEQIS AM+SLRKEAR+WFNEKGSK D+DDN + EE+AKASAWY VTYH YWGR
Sbjct: 1027 NDMEQISLAMKSLRKEARSWFNEKGSKSAYDEDDN--KNEEYAKASAWYRVTYHPDYWGR 1086
Query: 1081 YNEGMERDHFLSFPWCVSDKLIQIKKKK 1103
YNEGM+RDHFLSFPWCVSDKLIQIK++K
Sbjct: 1087 YNEGMQRDHFLSFPWCVSDKLIQIKREK 1103
BLAST of MS009042 vs. ExPASy Swiss-Prot
Match:
Q9LQV2 (RNA-dependent RNA polymerase 1 OS=Arabidopsis thaliana OX=3702 GN=RDR1 PE=2 SV=1)
HSP 1 Score: 1238.8 bits (3204), Expect = 0.0e+00
Identity = 644/1110 (58.02%), Postives = 804/1110 (72.43%), Query Frame = 0
Query: 1 MGKTIEIYGFRRDVTEEEVKEFLENHTGDGTVSEVRIAESRDENSRFKFATVRFTSELAA 60
MGKTI+++GF V+ EEVK+FLE TG GTV +++ + + R +A V+FTSE
Sbjct: 1 MGKTIQVFGFPNGVSAEEVKKFLERLTGSGTVYAIKVRQPKKGGPRV-YAIVQFTSERHT 60
Query: 61 GHVVSLAAAEERLWYGSSYLKAREADRQ-NRRGGVGVERMENLTLHLGSQISKEKLSVIW 120
+++ AA ERL+YG SYLKA E ++ + + + L + G Q+S +K +W
Sbjct: 61 RLIIT--AAAERLYYGRSYLKAFEVEQDIVPKPRASLHTISGLKMFFGCQVSTKKFLTLW 120
Query: 121 KGENCSVEFGSGVRKL---FVYVNLEYKMEVWFESILRVQLRCPPDGASKFLLIQLQGAP 180
++ V FG G+RKL F + +Y++E+ +E+I ++ L P +SKFL+IQ+ GAP
Sbjct: 121 SAQDVCVSFGIGMRKLHFSFSWYQKDYRLELSYENIWQIDLHSPQGRSSKFLVIQVIGAP 180
Query: 181 RIFKKRSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPGDQ 240
+IF+K + FY S S +WIR DFT SSCIGQS A CLEL
Sbjct: 181 KIFEKEDQPINLLFGIMD--FY---SDGSDEQWIRTTDFTSSSCIGQSTAFCLELPVHLN 240
Query: 241 LPSFFKTLVGY-EEIYGPFNLETGSSFSSTSN-LVPIVTPPQGFDLSYKILFKINALVQH 300
+P F + Y E F +E+GSS+SS +N LVP+V PP GF L ++ILFK+N LVQ+
Sbjct: 241 VPDFRENFANYAEHRASSFLIESGSSYSSNANTLVPVVDPPPGFSLPFEILFKLNTLVQN 300
Query: 301 GYLPGPLLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQNWLKQQYRSYYNSKQL 360
L GP LD +F+RL++ ++ I+ LEKLF+L EC YEP +WL+ +Y+ + + +L
Sbjct: 301 ACLSGPALDLDFYRLLNQKKYDRALIDHCLEKLFHLGECCYEPAHWLRDEYKKWISKGKL 360
Query: 361 PWKPDISLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELD 420
P P ISLDDGLVY++RVQ+TP++VYF GPE N+SNRV+R YS I+NF+RVSFVDE+L+
Sbjct: 361 PLSPTISLDDGLVYMYRVQVTPARVYFSGPEVNVSNRVLRHYSKYINNFLRVSFVDEDLE 420
Query: 421 KIRPIDLSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFAS 480
K+R +DLSPR+ST RT++YDRI S LR+GIVIGDKKFEFLAFS+SQLRENS WMFA
Sbjct: 421 KVRSMDLSPRSSTQ--RRTKLYDRIYSVLRDGIVIGDKKFEFLAFSSSQLRENSAWMFAP 480
Query: 481 REGLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETNNV 540
+ ++AA IR WMGDF IRNVAKYAARLGQSF SSR+TL V EIEVI DVE+ +
Sbjct: 481 IDRITAAHIRAWMGDFDHIRNVAKYAARLGQSFSSSRETLNVRSDEIEVIPDVEIISLGT 540
Query: 541 TYCFSDGIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPKSEKKLSLRKSM 600
Y FSDGIGKIS A KVA +CGL +PSAFQIRY GYKGVVA+DP S KKLSLRKSM
Sbjct: 541 RYVFSDGIGKISAEFARKVARKCGLTEFSPSAFQIRYGGYKGVVAVDPNSSKKLSLRKSM 600
Query: 601 LKYKSLDTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLDCILKDPS 660
K++S +T+LDVLAWSKY PC++NRQ+I LLSTLG+ D VFEKKQ+E + +LD IL P
Sbjct: 601 SKFESENTKLDVLAWSKYQPCYMNRQLITLLSTLGVTDSVFEKKQREVVDRLDAILTHPL 660
Query: 661 RALEVLELMSPGEMTSVLKEMLLY-YKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKG 720
A E L LM+PGE T++LK ++L YKP+ EPFL+MML+ FRA KLL+LRTK+RIF+ G
Sbjct: 661 EAHEALGLMAPGENTNILKALILCGYKPDAEPFLSMMLQNFRASKLLELRTKTRIFISGG 720
Query: 721 RTMMGCLDETGTLKYGQVFVQCSVPGRSSESNFIAEGKVVVAKNPCLHPGDMRVLNAVDV 780
R+MMGCLDET TL+YGQV VQ S P R FI G VVVAKNPCLHPGD+RVL AV+V
Sbjct: 721 RSMMGCLDETRTLEYGQVVVQYSDPMRPGR-RFIITGPVVVAKNPCLHPGDVRVLQAVNV 780
Query: 781 KALHHMVDCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELTCIRPVKPMSYKPAPTIQ 840
AL+HMVDCVVFPQKG RPHPNECSGSDLDGD+YFVCWD EL R +PM Y P PT
Sbjct: 781 PALNHMVDCVVFPQKGLRPHPNECSGSDLDGDIYFVCWDQELVPPRTSEPMDYTPEPTQI 840
Query: 841 LDHDVTIEEVQEYMANYMVNDGLGAIANAHTAFADREPRKAMSAKCIKLAKLFSIAVDFP 900
LDHDVTIEEV+EY ANY+VND LG IANAHTAFAD+EP KA S CI+LAK FS AVDFP
Sbjct: 841 LDHDVTIEEVEEYFANYIVNDSLGIIANAHTAFADKEPLKAFSDPCIELAKKFSTAVDFP 900
Query: 901 KTGVPANLPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREVA 960
KTGV A +P L V E+PDFMEK D+P YESK VIGKLFR VK+ + +I+++T +VA
Sbjct: 901 KTGVAAVIPQHLYVKEYPDFMEKPDKPTYESKNVIGKLFREVKERAPPLISIKSFTLDVA 960
Query: 961 AKCYDPDMEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFDK 1020
+K YD DME+DGFE+Y++EAF K YD+KLGNLMDYYGI+TE E++SG I+R+ KSF K
Sbjct: 961 SKSYDKDMEVDGFEEYVDEAFYQKANYDFKLGNLMDYYGIKTEAEILSGGIMRMSKSFTK 1020
Query: 1021 RTDMEQISHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEEEEFAKASAWYHVTYHQSYWG 1080
R D E I A+R+LRKE + FN + +EE E AKASAWYHVTYH SYWG
Sbjct: 1021 RRDAESIGRAVRALRKETLSLFNA-----------SEEEENESAKASAWYHVTYHSSYWG 1080
Query: 1081 RYNEGMERDHFLSFPWCVSDKLIQIKKKKI 1104
YNEG+ RDHFLSF WCV DKL++IKK +
Sbjct: 1081 LYNEGLNRDHFLSFAWCVYDKLVRIKKTNL 1088
BLAST of MS009042 vs. ExPASy Swiss-Prot
Match:
Q0DXS3 (Probable RNA-dependent RNA polymerase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=RDR1 PE=2 SV=2)
HSP 1 Score: 918.7 bits (2373), Expect = 6.3e-266
Identity = 453/728 (62.23%), Postives = 570/728 (78.30%), Query Frame = 0
Query: 377 SKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELDKIRPIDLSPRTSTA-NGERTRV 436
S VYF GPE N+SNRVVR +S DI+NF+R+SFVDE+ +K+R DLSPR+++ + RT +
Sbjct: 7 STVYFYGPEINVSNRVVRNFSSDIENFLRISFVDEDCEKLRATDLSPRSASGHDANRTAL 66
Query: 437 YDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFASREGLSAADIREWMGDFRQIRN 496
Y R+LS L +GI IG K FEFLAFS+SQLR+NS WMFASR+GL+A+DIR WMGDFR IRN
Sbjct: 67 YKRVLSVLSDGITIGGKNFEFLAFSSSQLRDNSAWMFASRQGLAASDIRTWMGDFRNIRN 126
Query: 497 VAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETNNVTYCFSDGIGKISETLAEKVAE 556
VAKYAARLGQSF SS +TL V+++E+E I+D++ N + FSDGIGKIS A +VA
Sbjct: 127 VAKYAARLGQSFSSSTETLKVQKYEVEEISDIK---NGTQHVFSDGIGKISSAFANEVAM 186
Query: 557 RCGLINHTPSAFQIRYAGYKGVVAIDPKSEKKLSLRKSMLKYKSLDTQLDVLAWSKYLPC 616
+C L PSAFQIRY GYKGVVA+DP S KLSLRKSMLK++S + +DVLA+SKY P
Sbjct: 187 KCNLKRFAPSAFQIRYGGYKGVVAVDPTSRWKLSLRKSMLKFQSDNITVDVLAYSKYQPG 246
Query: 617 FLNRQVINLLSTLGIKDRVFEKKQKEAIYQLDCILKDPSRALEVLELMSPGEMTSVLKEM 676
FLNRQ+I LLSTLG++D VFE+KQ+EA+ QL+ ++ DP A+E +ELM GE+T+ +KE+
Sbjct: 247 FLNRQLITLLSTLGVRDSVFEQKQEEAVNQLNKMVTDPQAAIEAIELMPMGEITNAVKEL 306
Query: 677 LLY-YKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDETGTLKYGQVFVQ 736
LL Y+P+ EP+L+M+L+TFRA KLL+L+TKSRI +PKGR MMGCLDET TLKYGQVF++
Sbjct: 307 LLCGYQPDDEPYLSMLLQTFRASKLLELKTKSRILIPKGRAMMGCLDETRTLKYGQVFIR 366
Query: 737 CSVPGRSSESNFIAEGKVVVAKNPCLHPGDMRVLNAVDVKALHHMVDCVVFPQKGQRPHP 796
+ G + F GKVV+AKNPCLHPGD+R+L+AVDV LHHM +CVVFPQ+G RPHP
Sbjct: 367 -ATSGVNDNDRFTVTGKVVIAKNPCLHPGDIRILHAVDVPVLHHMFNCVVFPQQGPRPHP 426
Query: 797 NECSGSDLDGDLYFVCWDPELTCIRPVKPMSYKPAPTIQLDHDVTIEEVQEYMANYMVND 856
NECSGSDLDGD+YFV WDP L R V PM Y PAPT LDHDVTIEEV+EY NY+VN+
Sbjct: 427 NECSGSDLDGDIYFVSWDPSLIPPRMVTPMDYTPAPTETLDHDVTIEEVEEYFTNYIVNE 486
Query: 857 GLGAIANAHTAFADREPRKAMSAKCIKLAKLFSIAVDFPKTGVPANLPPFLRVHEFPDFM 916
LG IANAH FAD+E KA S+ CI+LAKLFSIAVDFPKTGVPA +PP L V E+PDFM
Sbjct: 487 SLGMIANAHVVFADKEDLKAESSPCIELAKLFSIAVDFPKTGVPALIPPELHVKEYPDFM 546
Query: 917 EKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREVAAKCYDPDMEIDGFEKYLNEAF 976
EKLD+ YESKGVIGKL+R +K +T I+ +TREVA + YD DM +DG+E Y+ EA
Sbjct: 547 EKLDKVTYESKGVIGKLYREIKK---HTPHIKHFTREVARRSYDTDMIVDGYEDYITEAM 606
Query: 977 DFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFDKRTDMEQISHAMRSLRKEARAW 1036
K YD+KLGNLMD+YGI++E E++SG IL++ K+F K++D + I A+RSLRKEAR+
Sbjct: 607 ALKDEYDFKLGNLMDHYGIKSEAEIISGCILKMAKNFTKKSDADAIRLAVRSLRKEARSR 666
Query: 1037 FNEKGSKPRCDDDDNGDEEEEFAKASAWYHVTYHQSYWGRYNEGMERDHFLSFPWCVSDK 1096
F+E DD+ +G + E AKASAWYHVTYH +WG YNEG ER HF+SFPWC+ +K
Sbjct: 667 FSEMS----LDDNGHGHDASE-AKASAWYHVTYHPEFWGCYNEGYERPHFISFPWCIYEK 722
Query: 1097 LIQIKKKK 1103
L++IK+++
Sbjct: 727 LLRIKQRR 722
BLAST of MS009042 vs. ExPASy Swiss-Prot
Match:
O82504 (RNA-dependent RNA polymerase 2 OS=Arabidopsis thaliana OX=3702 GN=RDR2 PE=1 SV=1)
HSP 1 Score: 734.9 bits (1896), Expect = 1.3e-210
Identity = 443/1133 (39.10%), Postives = 652/1133 (57.55%), Query Frame = 0
Query: 4 TIEIYGFRRDVTEEEVKEFLENHTGDGTVSEVRIAESRDENSRFKFATVRFTSELAAGHV 63
T++I + + +E+ FLE H G+ TV + I +RD FA V+FT+ L
Sbjct: 11 TVKISNVPQTIVADELLRFLELHLGEDTVFALEIPTTRDNWKPRDFARVQFTT-LEVKSR 70
Query: 64 VSLAAAEERLWYGSSYLKAREA-DRQNRRGGVGVERMENLTLHLGSQISKEKLSVI---W 123
L +++ +L + + L+ EA D R +R++++ L +G S EK W
Sbjct: 71 AQLLSSQSKLLFKTHNLRLSEAYDDIIPRPVDPRKRLDDIVLTVGFPESDEKRFCALEKW 130
Query: 124 KGENCSVEFGSGVRKLFVYVNLE-YKMEVWFESILRVQLRCPPDGASKF--LLIQLQGAP 183
G C + + +V+ + + YK+EV FE I+ C AS+ L++L+ P
Sbjct: 131 DGVRCWILTEKRRVEFWVWESGDCYKIEVRFEDIIETLSCCVNGDASEIDAFLLKLKYGP 190
Query: 184 RIFKKRSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPGDQ 243
++FK+ + ++ S RF + WIR DF+ S IG S CLE+ G
Sbjct: 191 KVFKRVTVHIATKFKSDRYRFC---KEDFDFMWIRTTDFSGSKSIGTSTCFCLEVHNGST 250
Query: 244 LPSFFKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPP-QGFDLSYKILFKINALVQHG 303
+ F L Y E G +F+S + +VP++ G + Y+ILF++NALV
Sbjct: 251 MLDIFSGLPYYREDTLSLTYVDGKTFASAAQIVPLLNAAILGLEFPYEILFQLNALVHAQ 310
Query: 304 YLP-GPLLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQNWLKQQYRSYYNSKQL 363
+ D E +++ + L+KL Y+P ++K Q +S K++
Sbjct: 311 KISLFAASDMELIKILRGMSLETALV--ILKKLHQQSSICYDPVFFVKTQMQSVV--KKM 370
Query: 364 PWKPDIS----LDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVD 423
P + + ++ R +TPSK+Y GPE +N VV+ +++ + +F+RV+FV+
Sbjct: 371 KHSPASAYKRLTEQNIMSCQRAYVTPSKIYLLGPELETANYVVKNFAEHVSDFMRVTFVE 430
Query: 424 EELDKIRPIDLSPRTST---ANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLREN 483
E+ K+ LS + RT +Y+R+LS L GI +G K+FEFLAFSASQLR N
Sbjct: 431 EDWSKLPANALSVNSKEGYFVKPSRTNIYNRVLSILGEGITVGPKRFEFLAFSASQLRGN 490
Query: 484 SVWMFASREGLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDV 543
SVWMFAS E + A DIREWMG FR+IR+++K AAR+GQ F +SR+TL V ++E I D+
Sbjct: 491 SVWMFASNEKVKAEDIREWMGCFRKIRSISKCAARMGQLFSASRQTLIVRAQDVEQIPDI 550
Query: 544 EVETNNVTYCFSDGIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPKSEKK 603
EV T+ YCFSDGIGKIS A++VA++CGL +H PSAFQIRY GYKGV+A+D S +K
Sbjct: 551 EVTTDGADYCFSDGIGKISLAFAKQVAQKCGL-SHVPSAFQIRYGGYKGVIAVDRSSFRK 610
Query: 604 LSLRKSMLKYKSLDTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLD 663
LSLR SMLK+ S + L+V W++ +PCFLNR++I LLSTLGI+D +FE Q + L
Sbjct: 611 LSLRDSMLKFDSNNRMLNVTRWTESMPCFLNREIICLLSTLGIEDAMFEAMQAVHLSMLG 670
Query: 664 CILKDPSRALEVLELMSPGEMTSVLKEMLLY-YKPNKEPFLNMMLRTFRADKLLDLRTKS 723
+L+D AL VL+ +S ++L +MLL Y P+ EP+L+MMLR +L +L+++
Sbjct: 671 NMLEDRDAALNVLQKLSGENSKNLLVKMLLQGYAPSSEPYLSMMLRVHHESQLSELKSRC 730
Query: 724 RIFVPKGRTMMGCLDETGTLKYGQVFVQCSVP------------GRSSESNFIAEGKVVV 783
RI VPKGR ++GC+DE G L+YGQV+V+ ++ + E + GKVVV
Sbjct: 731 RILVPKGRILIGCMDEMGILEYGQVYVRVTLTKAELKSRDQSYFRKIDEETSVVIGKVVV 790
Query: 784 AKNPCLHPGDMRVLNAVDVKALHH--MVDCVVFPQKGQRPHPNECSGSDLDGDLYFVCWD 843
KNPCLHPGD+RVL+A+ +DC++FPQKG+RPHPNECSG DLDGD +FV WD
Sbjct: 791 TKNPCLHPGDIRVLDAIYEVHFEEKGYLDCIIFPQKGERPHPNECSGGDLDGDQFFVSWD 850
Query: 844 PELTCIRPVKPMSYKPAPTIQLDHDVTIEEVQEYMANYMVNDGLGAIANAHTAFADREPR 903
++ PM Y + +DHDVT+EE+ ++ +YM++D LG I+ AH ADR+P
Sbjct: 851 EKIIPSEMDPPMDYAGSRPRLMDHDVTLEEIHKFFVDYMISDTLGVISTAHLVHADRDPE 910
Query: 904 KAMSAKCIKLAKLFSIAVDFPKTGVPANLPPFLRVHEFPDFMEKLDRPAYESKGVIGKLF 963
KA S KC++LA L S AVDF KTG PA +P L+ EFPDF+E+ ++P Y S+ V GKL+
Sbjct: 911 KARSQKCLELANLHSRAVDFAKTGAPAEMPYALKPREFPDFLERFEKPTYISESVFGKLY 970
Query: 964 RSVKDVSFNTNTIETYTREVAAKCYDPDMEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYG 1023
R+VK S E + + A YD +E GFE ++ A + Y KL +LM YYG
Sbjct: 971 RAVKS-SLAQRKPEAESEDTVA--YDVTLEEAGFESFIETAKAHRDMYGEKLTSLMIYYG 1030
Query: 1024 IRTEPELVSGNILRVGKSFDKRTD------MEQISHAMRSLRKEARAWFNEKGSKPRCDD 1083
E E+++G IL+ + + R + ++I+ +++ L KEA WF + C+
Sbjct: 1031 AANEEEILTG-ILKTKEMYLARDNRRYGDMKDRITLSVKDLHKEAMGWFEKS-----CE- 1090
Query: 1084 DDNGDEEEEFAKASAWYHVTYHQSYWGRYNEGMERDHFLSFPWCVSDKLIQIK 1100
DE+++ ASAWY+VTY+ N E+ FLSFPW V D L+ IK
Sbjct: 1091 ----DEQQKKKLASAWYYVTYNP------NHRDEKLTFLSFPWIVGDVLLDIK 1114
BLAST of MS009042 vs. ExPASy Swiss-Prot
Match:
Q7XM31 (Probable RNA-dependent RNA polymerase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=RDR2 PE=2 SV=1)
HSP 1 Score: 697.2 bits (1798), Expect = 3.0e-199
Identity = 419/1087 (38.55%), Postives = 624/1087 (57.41%), Query Frame = 0
Query: 51 TVRFTSELAAGHVVSLAAAEERLWYGSSYLKA---------READRQNRRGGVGVERMEN 110
TV+F S AA LA++ + + L R +D R G G
Sbjct: 59 TVQFGSAAAAAAAAGLASSGRLPRFLGALLSVSPSPVDLLPRASDLSLRAAGAG------ 118
Query: 111 LTLHLGSQISKEKLSVIWKGENCSVEFGSGVRKLFVYV---NLEYKMEVWFESILRVQLR 170
L +G ++++ + E G R++ +Y+ + YK+EV FE ++ L
Sbjct: 119 --LVVGDRVAERVFEAADAWDGVRAEVIPGKRRVDLYLEHDSQRYKLEVLFED-MKDCLG 178
Query: 171 CPPDGASKFLLIQLQGAPRIFKKRSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPS 230
C DG +L+QL APRI S + +S RF+ + + S W+R +DFTP+
Sbjct: 179 CTLDGMGA-ILLQLNYAPRI-HTAISGPAVNSRFMDDRFHACKEDAKFS-WVRALDFTPN 238
Query: 231 SCIGQSFALCLELSPGDQLPSFFKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGF 290
G+ L L+L + K+L + G + + ++SN+VP+V P+ +
Sbjct: 239 YSFGRCSTLVLKLGKSALVSDILKSL-PFSGNLGELTMNSMDGVGASSNVVPLVHCPRDY 298
Query: 291 DLSYKILFKINALVQHGYLPGPLLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQ 350
+ Y++LF++N+L+ G + ++ + F+ + + R EK+ L+ Y P
Sbjct: 299 SVPYEVLFRLNSLMHMGKIVAKHVNADLFKAL--QELPVDVSRRIFEKMHKLESTCYGPL 358
Query: 351 NWLKQQYRSYYNSKQ--LPWKPDISLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQY 410
+++Q+ S S L + + + L+ +RV ITPSK++ GPE ++N VV+ +
Sbjct: 359 QFIQQEAYSMKRSHNVLLSNEGEGEGERKLMKCYRVNITPSKIFCFGPEEEVTNYVVKHH 418
Query: 411 SDDIDNFIRVSFVDEELDKIRPIDLSPRTST---ANGERTRVYDRILSTLRNGIVIGDKK 470
S +F+RV+FVDE+ K+ +S R + +T +Y RILS L+ G IG K
Sbjct: 419 SAYASDFVRVTFVDEDWSKLSSNAISARIEQGFFSKPFKTGLYYRILSILKKGFSIGPKN 478
Query: 471 FEFLAFSASQLRENSVWMFASREGLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKT 530
FEFLAFSASQLR NSVWMFAS L+A IR WMG F IR+V+K AAR+GQ F SSR+T
Sbjct: 479 FEFLAFSASQLRGNSVWMFASNASLNAGGIRRWMGHFENIRSVSKCAARMGQLFSSSRQT 538
Query: 531 LCVEEHEIEVITDVEVETNNVTYCFSDGIGKISETLAEKVAERCGL-INHTPSAFQIRYA 590
V ++EVI D+E+ T+ Y FSDGIGKIS A++VA GL + PSAFQIRY
Sbjct: 539 FEVLRWDVEVIPDIEITTDGSKYIFSDGIGKISLRFAKRVAHHVGLDPTNLPSAFQIRYG 598
Query: 591 GYKGVVAIDPKSEKKLSLRKSMLKYKSLDTQLDVLAWSKYLPCFLNRQVINLLSTLGIKD 650
GYKGV+AIDP S LSLR SM K++S L++ +WSK PC++NR++I+LLSTLGI+D
Sbjct: 599 GYKGVIAIDPMSSIDLSLRPSMKKFESESRMLNITSWSKSQPCYVNREIISLLSTLGIRD 658
Query: 651 RVFEKKQKEAIYQLDCILKDPSRALEVLELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLR 710
+F Q++ + + + +L + AL VL + E + +K +L Y+P+ EP+L+M+L+
Sbjct: 659 EIFVAMQQDEMRETEEMLTNKEVALSVLGKLGGSETKTAVKMLLQGYEPSSEPYLSMILK 718
Query: 711 TFRADKLLDLRTKSRIFVPKGRTMMGCLDETGTLKYGQVFVQCSVPGR----SSESNFIA 770
+ ++L D+RT+ +I VPKGR ++GCLDETG L+YGQV+++ + + S++S F
Sbjct: 719 AHQENRLTDIRTRCKIHVPKGRVLIGCLDETGVLEYGQVYIRITKNSKEQKDSNQSYFYN 778
Query: 771 E--------GKVVVAKNPCLHPGDMRVLNAVDVKALHHMVDCVVFPQKGQRPHPNECSGS 830
+ GKV + KNPCLHPGD+RVL A+ L MVDC+VFPQ+G+RPHPNECSG
Sbjct: 779 DDGKTATVVGKVAITKNPCLHPGDIRVLEAIYDPDLVGMVDCLVFPQRGERPHPNECSGG 838
Query: 831 DLDGDLYFVCWDPELTCIRPVKPMSYKPAPTIQLDHDVTIEEVQEYMANYMVNDGLGAIA 890
DLDGDLYF+ WD +L + PM Y +DH VT+EE+Q++ +YM+ND LGAI+
Sbjct: 839 DLDGDLYFITWDDKLIPEKVDTPMDYTATRPRIMDHVVTLEEIQKHFVDYMINDSLGAIS 898
Query: 891 NAHTAFADREPRKAMSAKCIKLAKLFSIAVDFPKTGVPANLPPFLRVHEFPDFMEKLDRP 950
AH ADR P KA S +C++LA L S+AVDF KTG PA +P LR E+PDFME+ ++P
Sbjct: 899 TAHLIHADRSPLKARSPECLQLATLHSMAVDFAKTGAPAEMPRTLRPREYPDFMERWEKP 958
Query: 951 AYESKGVIGKLFRSV---KDVSFNTNTIETYTREVAAKCYDPDMEIDGFEKYLNEAFDFK 1010
Y S GV+GKL+RS + S ++ + + + + + YDPD+E+ G +++L A ++
Sbjct: 959 MYISNGVLGKLYRSAMGHMEKSGDSGALSSSSAQ-PSPTYDPDLEVPGSDEFLQAAEEYY 1018
Query: 1011 TRYDYKLGNLMDYYGIRTEPELVSGNI----LRVGKSFDKRTDM-EQISHAMRSLRKEAR 1070
Y+ KL LM+YY E E+++GNI L + + + +M ++I A+ +L +EAR
Sbjct: 1019 ELYEEKLTTLMNYYRAELEDEILTGNIRNKMLYLKRDNKRYFEMKDRIVAAVDALHREAR 1078
Query: 1071 AWFNEKGSKPRCDDDDNGDEEEEFAKASAWYHVTYHQSYWGRYNEGMERDHFLSFPWCVS 1100
W + EE+ ASAWY VTYH + F SFPW
Sbjct: 1079 GWLL-----------SSRKEEDASRMASAWYRVTYHP-------DRRRGKRFWSFPWIAC 1111
BLAST of MS009042 vs. ExPASy Swiss-Prot
Match:
Q9SG02 (RNA-dependent RNA polymerase 6 OS=Arabidopsis thaliana OX=3702 GN=RDR6 PE=1 SV=1)
HSP 1 Score: 561.6 bits (1446), Expect = 2.0e-158
Identity = 379/1068 (35.49%), Postives = 562/1068 (52.62%), Query Frame = 0
Query: 98 RMENLTLHLGSQISKEKLSVIWKGENC-----------------SVEFGSGVRKLFVYVN 157
++ +TL +G+ +S++ V W+ E S F + +N
Sbjct: 131 KLAGITLEIGTLVSRDDFFVSWRAEGVDFLVDPFDNTCKFCFRKSTAFSFKDAVMHAVIN 190
Query: 158 LEYKMEVWFESILRVQLRCPPDGASKFLLIQLQGAPRIFKKRSSSSSSSSSSSSSRFYFK 217
+YK+E+ I V+ G L++QL +PR++ + + +
Sbjct: 191 CDYKLELLVRDIQTVRQYKTLHGF--VLILQLASSPRVWYRTADDDIYDTVPG------- 250
Query: 218 ESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPGDQ------LPSFFKTLVGYEEIYGPF 277
+ WIR DFT IG+ + + +SP + L F V E + P
Sbjct: 251 DLLDDDDPWIRTTDFTQVGAIGRCHSYRVLISPRYENKLRTALDYFRMRRVQEERVRWPP 310
Query: 278 NLETGSSFSS-TSNLVPIVTPPQGFDLSYKILFKINALVQHGYLPGPLLDDEFFRLVDPS 337
+ F S+ + +G +S++I+F +N+++ G L + FF L+
Sbjct: 311 RIRNEPCFGEPVSDHFFCIHHKEG--ISFEIMFLVNSVLHRGVFNQFQLTERFFDLL--- 370
Query: 338 RFRPEYIERA-LEKLFNLKECFYEPQNWLKQQYRSYYNSKQLPWKPDISLDDGLVYVHRV 397
R +P+ + A L+ L K ++ LK + +L + S D + + R+
Sbjct: 371 RNQPKDVNIASLKHLCTYKRPVFDAYKRLKLVQEWIQKNPKLLGSHEQSED--ISEIRRL 430
Query: 398 QITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELDKIRPIDLS--------PR 457
ITP++ Y PE LSNRV+R+Y + F+RV+F+DE + I LS
Sbjct: 431 VITPTRAYCLPPEVELSNRVLRRYKAVAERFLRVTFMDESMQTINSNVLSYFVAPIVKDL 490
Query: 458 TSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFASREGLSAADIR 517
TS++ ++T V+ R+ S L +G + +K+ FLAFSA+QLR+ S W FA +DI+
Sbjct: 491 TSSSFSQKTYVFKRVKSILTDGFKLCGRKYSFLAFSANQLRDRSAWFFAEDGKTRVSDIK 550
Query: 518 EWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDV-EVETNNVTYCFSDGIG 577
WMG F+ +NVAK AAR+G F S+ T+ V HE++ T+V ++E N Y FSDGIG
Sbjct: 551 TWMGKFKD-KNVAKCAARMGLCFSSTYATVDVMPHEVD--TEVPDIERNG--YVFSDGIG 610
Query: 578 KISETLAEKVAERCGL-INHTPSAFQIRYAGYKGVVAIDPKSEK--KLSLRKSMLKYKSL 637
I+ LA++V E+ L ++++P A+QIRYAG+KGVVA P +L+LR SM K+ S
Sbjct: 611 TITPDLADEVMEKLKLDVHYSPCAYQIRYAGFKGVVARWPSKSDGIRLALRDSMKKFFSK 670
Query: 638 DTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLDCILKDPSRALEVL 697
T L++ +W+++ P FLNRQ+I LLS LG+ D +F Q+ +Y+L+ IL D A EVL
Sbjct: 671 HTILEICSWTRFQPGFLNRQIITLLSVLGVPDEIFWDMQESMLYKLNRILDDTDVAFEVL 730
Query: 698 ELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCL 757
+ + + +KP EP L ML + R +L LR KSRIFV GR +MGCL
Sbjct: 731 TASCAEQGNTAAIMLSAGFKPKTEPHLRGMLSSVRIAQLWGLREKSRIFVTSGRWLMGCL 790
Query: 758 DETGTLKYGQVFVQCSVP----------GRSSESNF---IAEGKVVVAKNPCLHPGDMRV 817
DE G L++GQ F+Q S P R E+ + +G V +AKNPCLHPGD+R+
Sbjct: 791 DEAGILEHGQCFIQVSKPSIENCFSKHGSRFKETKTDLEVVKGYVAIAKNPCLHPGDVRI 850
Query: 818 LNAVDVKALHHMVDCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELTCIRPVK----P 877
L AVDV LHHM DC++FPQKG RPH NE SGSDLDGDLYFV WD +L I P +
Sbjct: 851 LEAVDVPQLHHMYDCLIFPQKGDRPHTNEASGSDLDGDLYFVAWDQKL--IPPNRKSYPA 910
Query: 878 MSYKPAPTIQLDHDVTIEEVQEYMANYMVNDGLGAIANAHTAFADREPRKAMSAKCIKLA 937
M Y A L V +++ ++ A + N+ LG I NAH ADR AM +C+ LA
Sbjct: 911 MHYDAAEEKSLGRAVNHQDIIDFFARNLANEQLGTICNAHVVHADRSEYGAMDEECLLLA 970
Query: 938 KLFSIAVDFPKTGVPANLPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDV-SFNT 997
+L + AVDFPKTG ++P L+ +PDFM K D Y+S ++G+L+R VK+V +
Sbjct: 971 ELAATAVDFPKTGKIVSMPFHLKPKLYPDFMGKEDYQTYKSNKILGRLYRRVKEVYDEDA 1030
Query: 998 NTIETYTREVAAKCYDPDMEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSG 1057
+ + +A YD +EI GFE + EA+ K YD +L L+ Y ++ E E+V+G
Sbjct: 1031 EASSEESTDPSAIPYDAVLEIPGFEDLIPEAWGHKCLYDGQLIGLLGQYKVQKEEEIVTG 1090
Query: 1058 NILRVGKSFDKRTD--MEQISHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEEEE----F 1100
+I + K K+ E++ H+ SL+KE R F E D +N EEE+
Sbjct: 1091 HIWSMPKYTSKKQGELKERLKHSYNSLKKEFRKVFEE-----TIPDHENLSEEEKNILYE 1150
BLAST of MS009042 vs. ExPASy TrEMBL
Match:
A0A6J1CZM7 (RNA-dependent RNA polymerase OS=Momordica charantia OX=3673 GN=LOC111015673 PE=3 SV=1)
HSP 1 Score: 2187.5 bits (5667), Expect = 0.0e+00
Identity = 1083/1103 (98.19%), Postives = 1088/1103 (98.64%), Query Frame = 0
Query: 1 MGKTIEIYGFRRDVTEEEVKEFLENHTGDGTVSEVRIAESRDENSRFKFATVRFTSELAA 60
MGKTIEIYGFRRDVT EEVKEFLENHTGDGTVS VRIAE RDENSRF FATV+F+SELAA
Sbjct: 1 MGKTIEIYGFRRDVTAEEVKEFLENHTGDGTVSAVRIAEFRDENSRFTFATVQFSSELAA 60
Query: 61 GHVVSLAAAEERLWYGSSYLKAREADRQNRRGGVGVERMENLTLHLGSQISKEKLSVIWK 120
GHVVS AAAEERLWYGSSYLKAREADRQNRRGGVGVERMENLTLHLGSQISKEKLSVIWK
Sbjct: 61 GHVVSQAAAEERLWYGSSYLKAREADRQNRRGGVGVERMENLTLHLGSQISKEKLSVIWK 120
Query: 121 GENCSVEFGSGVRKLFVYVNLEYKMEVWFESILRVQLRCPPDGASKFLLIQLQGAPRIFK 180
GENCSVEFGSGVRKLFVYVNLEYKMEVWFESILRVQLRCPPDGASKFLLIQLQ APRIFK
Sbjct: 121 GENCSVEFGSGVRKLFVYVNLEYKMEVWFESILRVQLRCPPDGASKFLLIQLQRAPRIFK 180
Query: 181 KRSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPGDQLPSF 240
K SSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPGDQLPSF
Sbjct: 181 KSSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPGDQLPSF 240
Query: 241 FKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGFDLSYKILFKINALVQHGYLPGP 300
FKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGFD S+KILFKINALVQHGYLPGP
Sbjct: 241 FKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGFDFSFKILFKINALVQHGYLPGP 300
Query: 301 LLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQNWLKQQYRSYYNSKQLPWKPDI 360
LLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQ WLKQQY SYYNSKQLPWKPDI
Sbjct: 301 LLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQKWLKQQYLSYYNSKQLPWKPDI 360
Query: 361 SLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELDKIRPID 420
SLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELDKIR ID
Sbjct: 361 SLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELDKIRSID 420
Query: 421 LSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFASREGLSA 480
LSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFASREGLSA
Sbjct: 421 LSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFASREGLSA 480
Query: 481 ADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETNNVTYCFSD 540
ADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETNNVTYCFSD
Sbjct: 481 ADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETNNVTYCFSD 540
Query: 541 GIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPKSEKKLSLRKSMLKYKSL 600
GIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDP SEKKLSLRKSMLKYKSL
Sbjct: 541 GIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPTSEKKLSLRKSMLKYKSL 600
Query: 601 DTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLDCILKDPSRALEVL 660
DTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQL+CILKDPSRALEVL
Sbjct: 601 DTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLNCILKDPSRALEVL 660
Query: 661 ELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCL 720
ELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCL
Sbjct: 661 ELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCL 720
Query: 721 DETGTLKYGQVFVQCSVPGRSSESNFIAEGKVVVAKNPCLHPGDMRVLNAVDVKALHHMV 780
DETGTLKYGQVFVQCSVPGRSSESNFI EGKVVVAKNPCLHPGDMRVLNAVDVKALHHMV
Sbjct: 721 DETGTLKYGQVFVQCSVPGRSSESNFIPEGKVVVAKNPCLHPGDMRVLNAVDVKALHHMV 780
Query: 781 DCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELTCIRPVKPMSYKPAPTIQLDHDVTI 840
DCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPEL CIRPVKPMSYKPAPTIQLDHDVTI
Sbjct: 781 DCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELNCIRPVKPMSYKPAPTIQLDHDVTI 840
Query: 841 EEVQEYMANYMVNDGLGAIANAHTAFADREPRKAMSAKCIKLAKLFSIAVDFPKTGVPAN 900
EEVQEYMANYMVNDGLGAIANAHT FADREPRKAMSAKCIKLAKLFSIAVDFPKTGVPAN
Sbjct: 841 EEVQEYMANYMVNDGLGAIANAHTVFADREPRKAMSAKCIKLAKLFSIAVDFPKTGVPAN 900
Query: 901 LPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREVAAKCYDPD 960
LPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREVAAKCYDPD
Sbjct: 901 LPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREVAAKCYDPD 960
Query: 961 MEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFDKRTDMEQI 1020
MEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFDKRTDMEQI
Sbjct: 961 MEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFDKRTDMEQI 1020
Query: 1021 SHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEEEEFAKASAWYHVTYHQSYWGRYNEGME 1080
SHAMRSLRKEARAWFNEKGSKPRCDDDDNGDE+EEFAKASAWYHVTYHQSYWGRYNEGME
Sbjct: 1021 SHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEDEEFAKASAWYHVTYHQSYWGRYNEGME 1080
Query: 1081 RDHFLSFPWCVSDKLIQIKKKKI 1104
RDHFLSFPWCVSDKLIQIKKKKI
Sbjct: 1081 RDHFLSFPWCVSDKLIQIKKKKI 1103
BLAST of MS009042 vs. ExPASy TrEMBL
Match:
A0A6J1K1Q3 (RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111489826 PE=3 SV=1)
HSP 1 Score: 1767.3 bits (4576), Expect = 0.0e+00
Identity = 875/1113 (78.62%), Postives = 976/1113 (87.69%), Query Frame = 0
Query: 1 MGKTIEIYGFRRDVTEEEVKEFLENHTGDGTVSEVRIAESRDENSRFKFATVRFTSELAA 60
MGK IEIYGF +VT +EVKEF+ENHTGDGTV VRI++ DE +RF FATVRFTS+L
Sbjct: 7 MGKMIEIYGFPAEVTADEVKEFIENHTGDGTVLTVRISKPNDEKARFTFATVRFTSKLGG 66
Query: 61 GHVVSLAAAEERLWYGSSYLKAREADRQNR--------RGGVGVERMENLTLHLGSQISK 120
+VV+ AAAE+RLW+GSSYLKAR+ +R+ R R G+ERMEN+ + GS +SK
Sbjct: 67 EYVVAQAAAEKRLWFGSSYLKARKVEREIRTATAAAAARVHGGLERMENVNVQWGSLVSK 126
Query: 121 EKLSVIWKGENCSVEFGSGVRKLFVYVN---LEYKMEVWFESILRVQLRCPPDGASKFLL 180
EK+ VIWK SVE+G G RKL Y++ +EYKME+ FE+IL VQLRC D S+ L
Sbjct: 127 EKMKVIWKEGKWSVEYGIGARKLGFYLSYEGVEYKMELCFENILGVQLRCSGDRGSELFL 186
Query: 181 IQLQGAPRIFKKRSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCL 240
IQLQGAPRIFKK SSSSS SS +S+G RWIRDVDFTPSSCIGQSFALCL
Sbjct: 187 IQLQGAPRIFKKTPSSSSSRLSS---------EESTGFRWIRDVDFTPSSCIGQSFALCL 246
Query: 241 ELSPGDQLPSFFKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGFDLSYKILFKIN 300
ELSPGDQLPSFF+TLVGY+E Y PF L GSS SS SNLVPI+TPPQGFD+SYKILFKIN
Sbjct: 247 ELSPGDQLPSFFRTLVGYKESYAPFILHPGSSLSSISNLVPIITPPQGFDISYKILFKIN 306
Query: 301 ALVQHGYLPGPLLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQNWLKQQYRSYY 360
AL+QHGYLPGP LD+EFFRLVD SRFRPEYIE ALEKLF+ KEC YEPQ WLKQQY S+Y
Sbjct: 307 ALLQHGYLPGPALDNEFFRLVDSSRFRPEYIEYALEKLFHFKECCYEPQKWLKQQYLSFY 366
Query: 361 NSKQLPWKPDISLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFV 420
+SKQLP KP++SLDDGLVYVHRVQITP+KVYFRGPEANLSNRVVR + DD+DNF+RVSFV
Sbjct: 367 SSKQLPCKPNVSLDDGLVYVHRVQITPTKVYFRGPEANLSNRVVRHFIDDVDNFLRVSFV 426
Query: 421 DEELDKIRPIDLSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSV 480
DEELDK+ IDLSPRTS N RTRVYDR+LS LRNGIVIGDKKFEFLAFSASQLRENS
Sbjct: 427 DEELDKLHSIDLSPRTSAENDLRTRVYDRVLSVLRNGIVIGDKKFEFLAFSASQLRENSC 486
Query: 481 WMFASREGLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEV 540
WMFASR+GLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSR+TLCVE+HEIEVI DVEV
Sbjct: 487 WMFASRKGLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRRTLCVEQHEIEVIPDVEV 546
Query: 541 ETNNVTYCFSDGIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPKSEKKLS 600
ETN +TYCFSDGIGKIS TLAEKVAE+CGLI+HTPSAFQIRYAGYKGVVAIDP S KKLS
Sbjct: 547 ETNKITYCFSDGIGKISGTLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSTKKLS 606
Query: 601 LRKSMLKYKSLDTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLDCI 660
LRKSMLKY SLDTQ+DVL+WSKY PCFLNRQVINLLSTLGIKDRVF KKQKEAI QLD I
Sbjct: 607 LRKSMLKYTSLDTQVDVLSWSKYQPCFLNRQVINLLSTLGIKDRVFVKKQKEAIDQLDSI 666
Query: 661 LKDPSRALEVLELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIF 720
L+DPSRALEVLELMSPGEMTS+LKE+LL YKPN+EPFLNMMLRTFRA KLLDLRTKSRIF
Sbjct: 667 LEDPSRALEVLELMSPGEMTSILKELLLLYKPNEEPFLNMMLRTFRAAKLLDLRTKSRIF 726
Query: 721 VPKGRTMMGCLDETGTLKYGQVFVQCSVPGRSSESNFIAEGKVVVAKNPCLHPGDMRVLN 780
VPKGRTMMGCLDET TL+YGQVFV CS+PGRSSESNF+ +GKVVVAKNPCLHPGD+R+L+
Sbjct: 727 VPKGRTMMGCLDETRTLEYGQVFVHCSLPGRSSESNFVVKGKVVVAKNPCLHPGDVRLLD 786
Query: 781 AVDVKALHHMVDCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELTCIRPVKPMSYKPA 840
AVDVKALHHMV+CVVFPQKG+RPHPNECSGSDLDGDLYFV WDPELT I+PVKPMSY+PA
Sbjct: 787 AVDVKALHHMVNCVVFPQKGERPHPNECSGSDLDGDLYFVGWDPELTRIKPVKPMSYEPA 846
Query: 841 PTIQLDHDVTIEEVQEYMANYMVNDGLGAIANAHTAFADREPRKAMSAKCIKLAKLFSIA 900
PT++LDHDVTIEEVQEY+A YMVNDGLG IANAHT FAD++P+KAM+A+CIKLAKLFSIA
Sbjct: 847 PTMRLDHDVTIEEVQEYLAKYMVNDGLGGIANAHTVFADKKPKKAMTAECIKLAKLFSIA 906
Query: 901 VDFPKTGVPANLPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYT 960
VDFPKTGVPAN P LRV E+PDFMEK D+P Y S GV+GKLFR VKDVS + NTIE ++
Sbjct: 907 VDFPKTGVPANFPHNLRVREYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDVNTIEIFS 966
Query: 961 REVAAKCYDPDMEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGK 1020
REVA KCYDPDME+DGFE YL+EAF++K RYD+KLGNLMDYYGI+TEPEL+SGNILR+ K
Sbjct: 967 REVATKCYDPDMEVDGFEDYLSEAFEYKARYDFKLGNLMDYYGIKTEPELISGNILRMAK 1026
Query: 1021 SFDKRTDMEQISHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEEEEFAKASAWYHVTYHQ 1080
SFDKR D+EQIS A++SLRKEAR+WFNEKGSK CD+D D++EE+AKASAWY VTYH
Sbjct: 1027 SFDKRNDLEQISLAIKSLRKEARSWFNEKGSKSACDED---DKDEEYAKASAWYRVTYHP 1086
Query: 1081 SYWGRYNEGMERDHFLSFPWCVSDKLIQIKKKK 1103
YWGRYNEGM+RDHFLSFPWCVSDKLIQIK++K
Sbjct: 1087 DYWGRYNEGMQRDHFLSFPWCVSDKLIQIKREK 1107
BLAST of MS009042 vs. ExPASy TrEMBL
Match:
A0A6J1FF44 (RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111444909 PE=3 SV=1)
HSP 1 Score: 1765.0 bits (4570), Expect = 0.0e+00
Identity = 873/1108 (78.79%), Postives = 974/1108 (87.91%), Query Frame = 0
Query: 1 MGKTIEIYGFRRDVTEEEVKEFLENHTGDGTVSEVRIAESRDENSRFKFATVRFTSELAA 60
MGKTIEIYGF VT +EVKEF+ENHTGDGTV VRI++ DE +RF FATV+FTS+L
Sbjct: 7 MGKTIEIYGFPTQVTADEVKEFIENHTGDGTVLTVRISKLNDEKARFTFATVQFTSKLGG 66
Query: 61 GHVVSLAAAEERLWYGSSYLKAREADRQNR---RGGVGVERMENLTLHLGSQISKEKLSV 120
+VV+ AAAE+RLW+GSSYLKAR+ +R+ + R +ERMEN+ + GS +SKE++ V
Sbjct: 67 EYVVAQAAAEKRLWFGSSYLKARKVEREIKTAARVHGELERMENVNVQWGSLVSKEEMKV 126
Query: 121 IWKGENCSVEFGSGVRKLFVYVN---LEYKMEVWFESILRVQLRCPPDGASKFLLIQLQG 180
IWKG SVE+G GVRKL Y++ +EYKME+ FE+IL VQLRC D SK LIQLQG
Sbjct: 127 IWKGGKWSVEYGIGVRKLGFYLSYEGVEYKMELCFENILGVQLRCSVDRGSKLFLIQLQG 186
Query: 181 APRIFKKRSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPG 240
APRIFKK SSSS SS +S+G RWIRDVDFTPSSCIGQSFALCLELS G
Sbjct: 187 APRIFKKAPLSSSSRLSS---------EESTGFRWIRDVDFTPSSCIGQSFALCLELSHG 246
Query: 241 DQLPSFFKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGFDLSYKILFKINALVQH 300
DQLPSFF+TLVGY+E Y PF L+TGSS SS SNLVPI+TPPQGFD+ YKILFKINAL+QH
Sbjct: 247 DQLPSFFQTLVGYKESYAPFILQTGSSLSSISNLVPIITPPQGFDIPYKILFKINALLQH 306
Query: 301 GYLPGPLLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQNWLKQQYRSYYNSKQL 360
GYLPGP LD+EFFRLVD SRFRP+Y+E AL+KLF+ KEC YEPQ WLKQQY S+Y+SKQL
Sbjct: 307 GYLPGPALDNEFFRLVDSSRFRPDYVEYALDKLFHFKECCYEPQKWLKQQYLSFYSSKQL 366
Query: 361 PWKPDISLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELD 420
PWKP+ISLDDGLVYVHRVQITP+KVYFRGPEANLSNRVVR + DD+DNF+RVSFVDEELD
Sbjct: 367 PWKPNISLDDGLVYVHRVQITPTKVYFRGPEANLSNRVVRHFIDDVDNFLRVSFVDEELD 426
Query: 421 KIRPIDLSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFAS 480
K+ IDLSPRTS N RTRVYDR+LS LRNGIVIGDKKFEFLAFSASQLRENS WMFAS
Sbjct: 427 KLHSIDLSPRTSAENDLRTRVYDRVLSVLRNGIVIGDKKFEFLAFSASQLRENSCWMFAS 486
Query: 481 REGLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETNNV 540
R+GLSAADIREWMGD RQIRNVAKYAARLGQSFGSSR+TLCVE+HEIEVI+DVEVETN +
Sbjct: 487 RKGLSAADIREWMGDLRQIRNVAKYAARLGQSFGSSRRTLCVEQHEIEVISDVEVETNKI 546
Query: 541 TYCFSDGIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPKSEKKLSLRKSM 600
TYCFSDGIGKIS TLAEKVAE+CGLI+HTPSAFQIRYAGYKGVVAIDP S KKLSLRKSM
Sbjct: 547 TYCFSDGIGKISGTLAEKVAEKCGLISHTPSAFQIRYAGYKGVVAIDPTSTKKLSLRKSM 606
Query: 601 LKYKSLDTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLDCILKDPS 660
LKY SLDTQ+DVL+WSKY PCFLNRQVINLLSTLGIKDRVF KKQKEAI QLD IL+DPS
Sbjct: 607 LKYTSLDTQVDVLSWSKYQPCFLNRQVINLLSTLGIKDRVFVKKQKEAIDQLDSILEDPS 666
Query: 661 RALEVLELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGR 720
RALEVLELMSPGEMTS+LKE+LL YKPN+EPFLNMMLRTFRA KLLDLRTKSRIFVPKGR
Sbjct: 667 RALEVLELMSPGEMTSILKELLLLYKPNEEPFLNMMLRTFRAAKLLDLRTKSRIFVPKGR 726
Query: 721 TMMGCLDETGTLKYGQVFVQCSVPGRSSESNFIAEGKVVVAKNPCLHPGDMRVLNAVDVK 780
TMMGCLDET TL+YGQVFV CS+PGRSSESNF+ +GKVVVAKNPCLHPGD+R+L+AVDVK
Sbjct: 727 TMMGCLDETRTLEYGQVFVHCSLPGRSSESNFVVKGKVVVAKNPCLHPGDVRLLDAVDVK 786
Query: 781 ALHHMVDCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELTCIRPVKPMSYKPAPTIQL 840
ALHHMVDCVVFPQKG+RPHPNECSGSDLDGDLYFV WDPELT I+PVKPMSY+PAPT+QL
Sbjct: 787 ALHHMVDCVVFPQKGKRPHPNECSGSDLDGDLYFVGWDPELTRIKPVKPMSYEPAPTMQL 846
Query: 841 DHDVTIEEVQEYMANYMVNDGLGAIANAHTAFADREPRKAMSAKCIKLAKLFSIAVDFPK 900
DHDVTIEEVQ Y+ YMVNDGLG IANAHT FAD++P+KAM+A+CIKLAKLFSIAVDFPK
Sbjct: 847 DHDVTIEEVQGYLVKYMVNDGLGGIANAHTVFADKKPKKAMAAECIKLAKLFSIAVDFPK 906
Query: 901 TGVPANLPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREVAA 960
TGVPAN P LRV E+PDFMEK D+P Y S GV+GKLFR VKDVS + NT+E ++REVA
Sbjct: 907 TGVPANFPHNLRVREYPDFMEKPDKPTYVSNGVLGKLFRGVKDVSSDVNTVEIFSREVAT 966
Query: 961 KCYDPDMEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFDKR 1020
KCYDPDME+DGFE YL+EAF+FK RYD+KLGNLMDYYGI+TEPEL+SGNILR+ KSFDKR
Sbjct: 967 KCYDPDMEVDGFEDYLSEAFEFKARYDFKLGNLMDYYGIKTEPELISGNILRMAKSFDKR 1026
Query: 1021 TDMEQISHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEEEEFAKASAWYHVTYHQSYWGR 1080
DMEQIS AM+SLRKEAR+WFNEKGSK D+DDN + EE+AKASAWY VTYH YWGR
Sbjct: 1027 NDMEQISLAMKSLRKEARSWFNEKGSKSAYDEDDN--KNEEYAKASAWYRVTYHPDYWGR 1086
Query: 1081 YNEGMERDHFLSFPWCVSDKLIQIKKKK 1103
YNEGM+RDHFLSFPWCVSDKLIQIK++K
Sbjct: 1087 YNEGMQRDHFLSFPWCVSDKLIQIKREK 1103
BLAST of MS009042 vs. ExPASy TrEMBL
Match:
A0A6J1JTB1 (RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111487656 PE=3 SV=1)
HSP 1 Score: 1750.3 bits (4532), Expect = 0.0e+00
Identity = 868/1107 (78.41%), Postives = 971/1107 (87.71%), Query Frame = 0
Query: 2 GKTIEIYGFRRDVTEEEVKEFLENHTGDGTVSEVRIAESRDENSRFKFATVRFTSELAAG 61
GKTIE++GFRR+V+ EVKEF+ENHTG+GTVS+VRI++ +D R FA V F S++AA
Sbjct: 5 GKTIEVFGFRREVSAGEVKEFMENHTGEGTVSKVRISKPKDGKGRLTFARVEFRSKVAAE 64
Query: 62 HVVSLAAAEERLWYGSSYLKAREADRQNRRGGVGVERMENLTLHLGSQISKEKLSVIWKG 121
+VV A AEE LW+G YLKAR +R+ R VERME + + LGS ISKE++ VIW+G
Sbjct: 65 YVVEKAEAEEGLWFGRWYLKARGVEREMR-----VERMEKVRVQLGSVISKEEMRVIWRG 124
Query: 122 ENCSVEFGSGVRKLFVYVNL-----EYKMEVWFESILRVQLRCPPDGASKFLLIQLQGAP 181
E SVE+G+G+RKL+ Y++ +YKME+ FE+IL VQLRCP + +S LIQLQGAP
Sbjct: 125 EEWSVEYGNGMRKLWFYLSYGVEDDDYKMELRFENILGVQLRCPHNSSSNLFLIQLQGAP 184
Query: 182 RIFKKRSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPGDQ 241
RIFKK SSSSSSS+FY KE S+G RWIRDVDFTPSSCIGQSF+LCLE+SPGDQ
Sbjct: 185 RIFKK-----CPSSSSSSSQFYSKE--STGFRWIRDVDFTPSSCIGQSFSLCLEVSPGDQ 244
Query: 242 LPSFFKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGFDLSYKILFKINALVQHGY 301
LPSFF+TLVGY+EIYGPF L TGS+FSSTSNLVPIVTPPQ FD+ YKILFKINALVQHGY
Sbjct: 245 LPSFFQTLVGYKEIYGPFKLSTGSAFSSTSNLVPIVTPPQPFDIPYKILFKINALVQHGY 304
Query: 302 LPGPLLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQNWLKQQYRSYYNSKQLPW 361
LPG LDD+FFRLVDPSRFR +YIE ALEKLF+LKEC YEPQ WLKQQY S+ +S QLPW
Sbjct: 305 LPGSALDDKFFRLVDPSRFRSDYIEYALEKLFSLKECCYEPQKWLKQQYLSFCSSMQLPW 364
Query: 362 KPDISLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELDKI 421
K +ISLDD LVYVHRVQITP+KVYFRGPEANLSNRVVR++ DDIDNF+RVSFVDEELDKI
Sbjct: 365 KANISLDDSLVYVHRVQITPAKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELDKI 424
Query: 422 RPIDLSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFASRE 481
DLSPRTST ERTRVYDRILS LRNGIVIG KKFEFLAFSASQLRENS WMFASRE
Sbjct: 425 HSSDLSPRTSTEKIERTRVYDRILSVLRNGIVIGGKKFEFLAFSASQLRENSFWMFASRE 484
Query: 482 GLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETNNVTY 541
GL+AADIREWMGDFR+IRNVAKYAARLGQSFGSSRKTLCVEE+EIEVI D EVE N+ Y
Sbjct: 485 GLNAADIREWMGDFREIRNVAKYAARLGQSFGSSRKTLCVEENEIEVIPDAEVERKNIKY 544
Query: 542 CFSDGIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPKSEKKLSLRKSMLK 601
CFSDGIGKISE LAE VAE+CGLI+HTPSAFQIRYAGYKGVVA+DP S+KKLSLRKSMLK
Sbjct: 545 CFSDGIGKISEALAEMVAEKCGLISHTPSAFQIRYAGYKGVVAVDPTSKKKLSLRKSMLK 604
Query: 602 YKSLDTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLDCILKDPSRA 661
Y SLDTQLDVL+WSKY PCFLNRQVINLLSTLGI+D VF K+QK+A+ QLD IL+DPS+A
Sbjct: 605 YMSLDTQLDVLSWSKYQPCFLNRQVINLLSTLGIRDDVFVKQQKKAVDQLDSILRDPSKA 664
Query: 662 LEVLELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTM 721
LEVLELMSPGEMTSVLK+MLL+Y P++EPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTM
Sbjct: 665 LEVLELMSPGEMTSVLKDMLLFYNPHEEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTM 724
Query: 722 MGCLDETGTLKYGQVFVQCSVPGRSSESNFIAEGKVVVAKNPCLHPGDMRVLNAVDVKAL 781
MGCLDET TL+YGQVF+ CSVPGRSSESNF+ +GKVVVAKNPCLHPGD+RVL+AVDVK L
Sbjct: 725 MGCLDETRTLEYGQVFIHCSVPGRSSESNFVVKGKVVVAKNPCLHPGDVRVLDAVDVKTL 784
Query: 782 HHMVDCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELTCIRPVKPMSYKPAPTIQLDH 841
HHMVDCVVFPQKG+RPHPNECSGSDLDGDLYFVCWDPELTCIRP+KPMSY+PAPTIQLDH
Sbjct: 785 HHMVDCVVFPQKGKRPHPNECSGSDLDGDLYFVCWDPELTCIRPIKPMSYEPAPTIQLDH 844
Query: 842 DVTIEEVQEYMANYMVNDGLGAIANAHTAFADREPRKAMSAKCIKLAKLFSIAVDFPKTG 901
DVT+EEVQEY ANYMVNDGLG IANAHT FAD +P+KAMSA+CI+LAKLFSIAVDFPKTG
Sbjct: 845 DVTLEEVQEYFANYMVNDGLGGIANAHTVFADMKPKKAMSAECIELAKLFSIAVDFPKTG 904
Query: 902 VPANLPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREVAAKC 961
VPANLP LRVHE+PDFMEK ++ Y S GVIGKLFR VKDVS + N IET+TREVA KC
Sbjct: 905 VPANLPRSLRVHEYPDFMEKPNKRTYISNGVIGKLFRGVKDVSSDINNIETFTREVAVKC 964
Query: 962 YDPDMEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFDKRTD 1021
YDPDME+ GFE YL+EAFD+K YD+KLGNLMDYYGI TEPELVSGNILR+GKSFDKR D
Sbjct: 965 YDPDMEVMGFENYLSEAFDYKRMYDFKLGNLMDYYGIETEPELVSGNILRMGKSFDKRND 1024
Query: 1022 MEQISHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEEEEFAKASAWYHVTYHQSYWGRYN 1081
MEQIS AMRSLRKEAR WFNEK SK ++N E+ E+AKASAWYHVTYH ++WGRYN
Sbjct: 1025 MEQISLAMRSLRKEARGWFNEKASKSIY--NNNKGEDNEYAKASAWYHVTYHPNFWGRYN 1084
Query: 1082 EGMERDHFLSFPWCVSDKLIQIKKKKI 1104
EGM+RDHFLSFPWCVSDKLIQIK++K+
Sbjct: 1085 EGMQRDHFLSFPWCVSDKLIQIKREKM 1097
BLAST of MS009042 vs. ExPASy TrEMBL
Match:
A0A6J1EB47 (RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111430971 PE=3 SV=1)
HSP 1 Score: 1746.5 bits (4522), Expect = 0.0e+00
Identity = 866/1109 (78.09%), Postives = 977/1109 (88.10%), Query Frame = 0
Query: 2 GKTIEIYGFRRDVTEEEVKEFLENHTGDGTVSEVRIAESRDENSRFKFATVRFTSELAAG 61
GKTIE++GF R+V+ EVKEF+ENHTG+GTVS+VRI++ + E SR A V F S++AA
Sbjct: 5 GKTIEVFGFGREVSAGEVKEFMENHTGEGTVSKVRISKPKYEKSRLTSARVEFRSKVAAE 64
Query: 62 HVVSLAAAEERLWYGSSYLKAREADRQNRR--GGVGVERMENLTLHLGSQISKEKLSVIW 121
+VV A AEERLW+G YLKAR +R+ R +ER+E + + LGS I K+++ VIW
Sbjct: 65 YVVEKAEAEERLWFGRWYLKARRVEREREREMRVERLERLEKVRVQLGSVIWKDEMRVIW 124
Query: 122 KGENCSVEFGSGVRKLFVYVNL-----EYKMEVWFESILRVQLRCPPDGASKFLLIQLQG 181
+GE+ SVE+G+G+RKL+ Y++ +YKME+ FE+IL VQLRCP + +S LIQLQG
Sbjct: 125 RGEDWSVEYGNGMRKLWFYLSYGVEDDDYKMELRFENILGVQLRCPHNSSSNLFLIQLQG 184
Query: 182 APRIFKKRSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPG 241
APRIFKK SSSSSSSS+FY KE S+G RWIRDVDFTPSSCIGQSFALC E+SPG
Sbjct: 185 APRIFKK----CPSSSSSSSSQFYSKE--STGFRWIRDVDFTPSSCIGQSFALCFEVSPG 244
Query: 242 DQLPSFFKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPPQGFDLSYKILFKINALVQH 301
DQLPSFF+TLVGY+EIYGPF L TGS+FSSTSNLVPIVTPPQ F + YKILFKINALVQH
Sbjct: 245 DQLPSFFQTLVGYKEIYGPFKLSTGSAFSSTSNLVPIVTPPQPFHIPYKILFKINALVQH 304
Query: 302 GYLPGPLLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQNWLKQQYRSYYNSKQL 361
GYLPG LDD+FFRLVDP+RFR +YIE ALEKLF+LKEC YEPQ WLKQQY S+ +S QL
Sbjct: 305 GYLPGSALDDKFFRLVDPTRFRSDYIEYALEKLFSLKECCYEPQKWLKQQYLSFCSSMQL 364
Query: 362 PWKPDISLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELD 421
PWK +ISLDD LVYVHRVQITP+KVYFRGPEANLSNRVVR++ DDIDNF+RVSFVDEELD
Sbjct: 365 PWKANISLDDSLVYVHRVQITPAKVYFRGPEANLSNRVVRRFIDDIDNFLRVSFVDEELD 424
Query: 422 KIRPIDLSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFAS 481
KI DLSPRTST ERTRVYDRILS LRNGIVIG KKFEFLAFSASQLRENS WMFAS
Sbjct: 425 KIYSSDLSPRTSTEKIERTRVYDRILSVLRNGIVIGGKKFEFLAFSASQLRENSFWMFAS 484
Query: 482 REGLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETNNV 541
REGL+AADIREWMGDFR+IRNVAKYAARLGQSFGSSRKTLCVEE+EIEVI DVEVE N+
Sbjct: 485 REGLNAADIREWMGDFREIRNVAKYAARLGQSFGSSRKTLCVEENEIEVIPDVEVERKNI 544
Query: 542 TYCFSDGIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPKSEKKLSLRKSM 601
YCFSDGIGKISE LAE VAE+CGLI+HTPSAFQIRYAGYKGVVA+DP S+KKLSLRKSM
Sbjct: 545 KYCFSDGIGKISEALAEMVAEKCGLISHTPSAFQIRYAGYKGVVAVDPTSKKKLSLRKSM 604
Query: 602 LKYKSLDTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLDCILKDPS 661
LKY SLDTQLDVL+WSKY PCFLNRQVINLLSTLGI+D VF K+QK+A+ QLD IL+DPS
Sbjct: 605 LKYMSLDTQLDVLSWSKYQPCFLNRQVINLLSTLGIQDDVFVKQQKKAVDQLDSILRDPS 664
Query: 662 RALEVLELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGR 721
+ALEVLELMSPGEMTSVLK+MLL+Y P++EPFLNMMLRTFRADKLLDLRTKSRIFVP+GR
Sbjct: 665 KALEVLELMSPGEMTSVLKDMLLFYNPHEEPFLNMMLRTFRADKLLDLRTKSRIFVPEGR 724
Query: 722 TMMGCLDETGTLKYGQVFVQCSVPGRSSESNFIAEGKVVVAKNPCLHPGDMRVLNAVDVK 781
TMMGCLDET TL+YGQVF+ CSVPGRSSESNF+ +GKVVVAKNPCLHPGD+RVL+AVDVK
Sbjct: 725 TMMGCLDETRTLEYGQVFIHCSVPGRSSESNFVVKGKVVVAKNPCLHPGDVRVLDAVDVK 784
Query: 782 ALHHMVDCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELTCIRPVKPMSYKPAPTIQL 841
LHHMVDCVVFPQKG+RPHPNECSGSDLDGDLYFVCWDPELTCIRP+KPMSY+PAPTIQL
Sbjct: 785 TLHHMVDCVVFPQKGKRPHPNECSGSDLDGDLYFVCWDPELTCIRPIKPMSYEPAPTIQL 844
Query: 842 DHDVTIEEVQEYMANYMVNDGLGAIANAHTAFADREPRKAMSAKCIKLAKLFSIAVDFPK 901
DHDVTIEEVQEY ANYMVNDGLG IANAHT FAD++P+KAMSA+CI+LAKLFSIAVDFPK
Sbjct: 845 DHDVTIEEVQEYFANYMVNDGLGGIANAHTVFADKKPKKAMSAECIELAKLFSIAVDFPK 904
Query: 902 TGVPANLPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREVAA 961
TGVPANLP LRVHE+PDFMEK ++ Y S GVIGKLFR VKDVS + N IET+TREVAA
Sbjct: 905 TGVPANLPRSLRVHEYPDFMEKPNKRTYTSNGVIGKLFRGVKDVSSDINNIETFTREVAA 964
Query: 962 KCYDPDMEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFDKR 1021
KCYDPDME+ GFE YL+EAFD+K+ YD+KLGNLMDYYGI TEPELVSGNILR+GKSFDKR
Sbjct: 965 KCYDPDMEVMGFENYLSEAFDYKSMYDFKLGNLMDYYGIETEPELVSGNILRMGKSFDKR 1024
Query: 1022 TDMEQISHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEEEEFAKASAWYHVTYHQSYWGR 1081
DMEQIS AMRSLRKEAR WFNEKGSK ++N DE++E+AKASAWYHVTYH ++WGR
Sbjct: 1025 NDMEQISLAMRSLRKEARGWFNEKGSKSIY--NNNKDEDDEYAKASAWYHVTYHPNFWGR 1084
Query: 1082 YNEGMERDHFLSFPWCVSDKLIQIKKKKI 1104
YNEGM+RDHFLSFPWCVS+KLIQIK++K+
Sbjct: 1085 YNEGMQRDHFLSFPWCVSEKLIQIKREKM 1105
BLAST of MS009042 vs. TAIR 10
Match:
AT1G14790.1 (RNA-dependent RNA polymerase 1 )
HSP 1 Score: 1238.8 bits (3204), Expect = 0.0e+00
Identity = 644/1110 (58.02%), Postives = 804/1110 (72.43%), Query Frame = 0
Query: 1 MGKTIEIYGFRRDVTEEEVKEFLENHTGDGTVSEVRIAESRDENSRFKFATVRFTSELAA 60
MGKTI+++GF V+ EEVK+FLE TG GTV +++ + + R +A V+FTSE
Sbjct: 1 MGKTIQVFGFPNGVSAEEVKKFLERLTGSGTVYAIKVRQPKKGGPRV-YAIVQFTSERHT 60
Query: 61 GHVVSLAAAEERLWYGSSYLKAREADRQ-NRRGGVGVERMENLTLHLGSQISKEKLSVIW 120
+++ AA ERL+YG SYLKA E ++ + + + L + G Q+S +K +W
Sbjct: 61 RLIIT--AAAERLYYGRSYLKAFEVEQDIVPKPRASLHTISGLKMFFGCQVSTKKFLTLW 120
Query: 121 KGENCSVEFGSGVRKL---FVYVNLEYKMEVWFESILRVQLRCPPDGASKFLLIQLQGAP 180
++ V FG G+RKL F + +Y++E+ +E+I ++ L P +SKFL+IQ+ GAP
Sbjct: 121 SAQDVCVSFGIGMRKLHFSFSWYQKDYRLELSYENIWQIDLHSPQGRSSKFLVIQVIGAP 180
Query: 181 RIFKKRSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPGDQ 240
+IF+K + FY S S +WIR DFT SSCIGQS A CLEL
Sbjct: 181 KIFEKEDQPINLLFGIMD--FY---SDGSDEQWIRTTDFTSSSCIGQSTAFCLELPVHLN 240
Query: 241 LPSFFKTLVGY-EEIYGPFNLETGSSFSSTSN-LVPIVTPPQGFDLSYKILFKINALVQH 300
+P F + Y E F +E+GSS+SS +N LVP+V PP GF L ++ILFK+N LVQ+
Sbjct: 241 VPDFRENFANYAEHRASSFLIESGSSYSSNANTLVPVVDPPPGFSLPFEILFKLNTLVQN 300
Query: 301 GYLPGPLLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQNWLKQQYRSYYNSKQL 360
L GP LD +F+RL++ ++ I+ LEKLF+L EC YEP +WL+ +Y+ + + +L
Sbjct: 301 ACLSGPALDLDFYRLLNQKKYDRALIDHCLEKLFHLGECCYEPAHWLRDEYKKWISKGKL 360
Query: 361 PWKPDISLDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELD 420
P P ISLDDGLVY++RVQ+TP++VYF GPE N+SNRV+R YS I+NF+RVSFVDE+L+
Sbjct: 361 PLSPTISLDDGLVYMYRVQVTPARVYFSGPEVNVSNRVLRHYSKYINNFLRVSFVDEDLE 420
Query: 421 KIRPIDLSPRTSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFAS 480
K+R +DLSPR+ST RT++YDRI S LR+GIVIGDKKFEFLAFS+SQLRENS WMFA
Sbjct: 421 KVRSMDLSPRSSTQ--RRTKLYDRIYSVLRDGIVIGDKKFEFLAFSSSQLRENSAWMFAP 480
Query: 481 REGLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDVEVETNNV 540
+ ++AA IR WMGDF IRNVAKYAARLGQSF SSR+TL V EIEVI DVE+ +
Sbjct: 481 IDRITAAHIRAWMGDFDHIRNVAKYAARLGQSFSSSRETLNVRSDEIEVIPDVEIISLGT 540
Query: 541 TYCFSDGIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPKSEKKLSLRKSM 600
Y FSDGIGKIS A KVA +CGL +PSAFQIRY GYKGVVA+DP S KKLSLRKSM
Sbjct: 541 RYVFSDGIGKISAEFARKVARKCGLTEFSPSAFQIRYGGYKGVVAVDPNSSKKLSLRKSM 600
Query: 601 LKYKSLDTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLDCILKDPS 660
K++S +T+LDVLAWSKY PC++NRQ+I LLSTLG+ D VFEKKQ+E + +LD IL P
Sbjct: 601 SKFESENTKLDVLAWSKYQPCYMNRQLITLLSTLGVTDSVFEKKQREVVDRLDAILTHPL 660
Query: 661 RALEVLELMSPGEMTSVLKEMLLY-YKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKG 720
A E L LM+PGE T++LK ++L YKP+ EPFL+MML+ FRA KLL+LRTK+RIF+ G
Sbjct: 661 EAHEALGLMAPGENTNILKALILCGYKPDAEPFLSMMLQNFRASKLLELRTKTRIFISGG 720
Query: 721 RTMMGCLDETGTLKYGQVFVQCSVPGRSSESNFIAEGKVVVAKNPCLHPGDMRVLNAVDV 780
R+MMGCLDET TL+YGQV VQ S P R FI G VVVAKNPCLHPGD+RVL AV+V
Sbjct: 721 RSMMGCLDETRTLEYGQVVVQYSDPMRPGR-RFIITGPVVVAKNPCLHPGDVRVLQAVNV 780
Query: 781 KALHHMVDCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELTCIRPVKPMSYKPAPTIQ 840
AL+HMVDCVVFPQKG RPHPNECSGSDLDGD+YFVCWD EL R +PM Y P PT
Sbjct: 781 PALNHMVDCVVFPQKGLRPHPNECSGSDLDGDIYFVCWDQELVPPRTSEPMDYTPEPTQI 840
Query: 841 LDHDVTIEEVQEYMANYMVNDGLGAIANAHTAFADREPRKAMSAKCIKLAKLFSIAVDFP 900
LDHDVTIEEV+EY ANY+VND LG IANAHTAFAD+EP KA S CI+LAK FS AVDFP
Sbjct: 841 LDHDVTIEEVEEYFANYIVNDSLGIIANAHTAFADKEPLKAFSDPCIELAKKFSTAVDFP 900
Query: 901 KTGVPANLPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDVSFNTNTIETYTREVA 960
KTGV A +P L V E+PDFMEK D+P YESK VIGKLFR VK+ + +I+++T +VA
Sbjct: 901 KTGVAAVIPQHLYVKEYPDFMEKPDKPTYESKNVIGKLFREVKERAPPLISIKSFTLDVA 960
Query: 961 AKCYDPDMEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSGNILRVGKSFDK 1020
+K YD DME+DGFE+Y++EAF K YD+KLGNLMDYYGI+TE E++SG I+R+ KSF K
Sbjct: 961 SKSYDKDMEVDGFEEYVDEAFYQKANYDFKLGNLMDYYGIKTEAEILSGGIMRMSKSFTK 1020
Query: 1021 RTDMEQISHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEEEEFAKASAWYHVTYHQSYWG 1080
R D E I A+R+LRKE + FN + +EE E AKASAWYHVTYH SYWG
Sbjct: 1021 RRDAESIGRAVRALRKETLSLFNA-----------SEEEENESAKASAWYHVTYHSSYWG 1080
Query: 1081 RYNEGMERDHFLSFPWCVSDKLIQIKKKKI 1104
YNEG+ RDHFLSF WCV DKL++IKK +
Sbjct: 1081 LYNEGLNRDHFLSFAWCVYDKLVRIKKTNL 1088
BLAST of MS009042 vs. TAIR 10
Match:
AT4G11130.1 (RNA-dependent RNA polymerase 2 )
HSP 1 Score: 734.9 bits (1896), Expect = 9.2e-212
Identity = 443/1133 (39.10%), Postives = 652/1133 (57.55%), Query Frame = 0
Query: 4 TIEIYGFRRDVTEEEVKEFLENHTGDGTVSEVRIAESRDENSRFKFATVRFTSELAAGHV 63
T++I + + +E+ FLE H G+ TV + I +RD FA V+FT+ L
Sbjct: 11 TVKISNVPQTIVADELLRFLELHLGEDTVFALEIPTTRDNWKPRDFARVQFTT-LEVKSR 70
Query: 64 VSLAAAEERLWYGSSYLKAREA-DRQNRRGGVGVERMENLTLHLGSQISKEKLSVI---W 123
L +++ +L + + L+ EA D R +R++++ L +G S EK W
Sbjct: 71 AQLLSSQSKLLFKTHNLRLSEAYDDIIPRPVDPRKRLDDIVLTVGFPESDEKRFCALEKW 130
Query: 124 KGENCSVEFGSGVRKLFVYVNLE-YKMEVWFESILRVQLRCPPDGASKF--LLIQLQGAP 183
G C + + +V+ + + YK+EV FE I+ C AS+ L++L+ P
Sbjct: 131 DGVRCWILTEKRRVEFWVWESGDCYKIEVRFEDIIETLSCCVNGDASEIDAFLLKLKYGP 190
Query: 184 RIFKKRSSSSSSSSSSSSSRFYFKESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPGDQ 243
++FK+ + ++ S RF + WIR DF+ S IG S CLE+ G
Sbjct: 191 KVFKRVTVHIATKFKSDRYRFC---KEDFDFMWIRTTDFSGSKSIGTSTCFCLEVHNGST 250
Query: 244 LPSFFKTLVGYEEIYGPFNLETGSSFSSTSNLVPIVTPP-QGFDLSYKILFKINALVQHG 303
+ F L Y E G +F+S + +VP++ G + Y+ILF++NALV
Sbjct: 251 MLDIFSGLPYYREDTLSLTYVDGKTFASAAQIVPLLNAAILGLEFPYEILFQLNALVHAQ 310
Query: 304 YLP-GPLLDDEFFRLVDPSRFRPEYIERALEKLFNLKECFYEPQNWLKQQYRSYYNSKQL 363
+ D E +++ + L+KL Y+P ++K Q +S K++
Sbjct: 311 KISLFAASDMELIKILRGMSLETALV--ILKKLHQQSSICYDPVFFVKTQMQSVV--KKM 370
Query: 364 PWKPDIS----LDDGLVYVHRVQITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVD 423
P + + ++ R +TPSK+Y GPE +N VV+ +++ + +F+RV+FV+
Sbjct: 371 KHSPASAYKRLTEQNIMSCQRAYVTPSKIYLLGPELETANYVVKNFAEHVSDFMRVTFVE 430
Query: 424 EELDKIRPIDLSPRTST---ANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLREN 483
E+ K+ LS + RT +Y+R+LS L GI +G K+FEFLAFSASQLR N
Sbjct: 431 EDWSKLPANALSVNSKEGYFVKPSRTNIYNRVLSILGEGITVGPKRFEFLAFSASQLRGN 490
Query: 484 SVWMFASREGLSAADIREWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDV 543
SVWMFAS E + A DIREWMG FR+IR+++K AAR+GQ F +SR+TL V ++E I D+
Sbjct: 491 SVWMFASNEKVKAEDIREWMGCFRKIRSISKCAARMGQLFSASRQTLIVRAQDVEQIPDI 550
Query: 544 EVETNNVTYCFSDGIGKISETLAEKVAERCGLINHTPSAFQIRYAGYKGVVAIDPKSEKK 603
EV T+ YCFSDGIGKIS A++VA++CGL +H PSAFQIRY GYKGV+A+D S +K
Sbjct: 551 EVTTDGADYCFSDGIGKISLAFAKQVAQKCGL-SHVPSAFQIRYGGYKGVIAVDRSSFRK 610
Query: 604 LSLRKSMLKYKSLDTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLD 663
LSLR SMLK+ S + L+V W++ +PCFLNR++I LLSTLGI+D +FE Q + L
Sbjct: 611 LSLRDSMLKFDSNNRMLNVTRWTESMPCFLNREIICLLSTLGIEDAMFEAMQAVHLSMLG 670
Query: 664 CILKDPSRALEVLELMSPGEMTSVLKEMLLY-YKPNKEPFLNMMLRTFRADKLLDLRTKS 723
+L+D AL VL+ +S ++L +MLL Y P+ EP+L+MMLR +L +L+++
Sbjct: 671 NMLEDRDAALNVLQKLSGENSKNLLVKMLLQGYAPSSEPYLSMMLRVHHESQLSELKSRC 730
Query: 724 RIFVPKGRTMMGCLDETGTLKYGQVFVQCSVP------------GRSSESNFIAEGKVVV 783
RI VPKGR ++GC+DE G L+YGQV+V+ ++ + E + GKVVV
Sbjct: 731 RILVPKGRILIGCMDEMGILEYGQVYVRVTLTKAELKSRDQSYFRKIDEETSVVIGKVVV 790
Query: 784 AKNPCLHPGDMRVLNAVDVKALHH--MVDCVVFPQKGQRPHPNECSGSDLDGDLYFVCWD 843
KNPCLHPGD+RVL+A+ +DC++FPQKG+RPHPNECSG DLDGD +FV WD
Sbjct: 791 TKNPCLHPGDIRVLDAIYEVHFEEKGYLDCIIFPQKGERPHPNECSGGDLDGDQFFVSWD 850
Query: 844 PELTCIRPVKPMSYKPAPTIQLDHDVTIEEVQEYMANYMVNDGLGAIANAHTAFADREPR 903
++ PM Y + +DHDVT+EE+ ++ +YM++D LG I+ AH ADR+P
Sbjct: 851 EKIIPSEMDPPMDYAGSRPRLMDHDVTLEEIHKFFVDYMISDTLGVISTAHLVHADRDPE 910
Query: 904 KAMSAKCIKLAKLFSIAVDFPKTGVPANLPPFLRVHEFPDFMEKLDRPAYESKGVIGKLF 963
KA S KC++LA L S AVDF KTG PA +P L+ EFPDF+E+ ++P Y S+ V GKL+
Sbjct: 911 KARSQKCLELANLHSRAVDFAKTGAPAEMPYALKPREFPDFLERFEKPTYISESVFGKLY 970
Query: 964 RSVKDVSFNTNTIETYTREVAAKCYDPDMEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYG 1023
R+VK S E + + A YD +E GFE ++ A + Y KL +LM YYG
Sbjct: 971 RAVKS-SLAQRKPEAESEDTVA--YDVTLEEAGFESFIETAKAHRDMYGEKLTSLMIYYG 1030
Query: 1024 IRTEPELVSGNILRVGKSFDKRTD------MEQISHAMRSLRKEARAWFNEKGSKPRCDD 1083
E E+++G IL+ + + R + ++I+ +++ L KEA WF + C+
Sbjct: 1031 AANEEEILTG-ILKTKEMYLARDNRRYGDMKDRITLSVKDLHKEAMGWFEKS-----CE- 1090
Query: 1084 DDNGDEEEEFAKASAWYHVTYHQSYWGRYNEGMERDHFLSFPWCVSDKLIQIK 1100
DE+++ ASAWY+VTY+ N E+ FLSFPW V D L+ IK
Sbjct: 1091 ----DEQQKKKLASAWYYVTYNP------NHRDEKLTFLSFPWIVGDVLLDIK 1114
BLAST of MS009042 vs. TAIR 10
Match:
AT3G49500.1 (RNA-dependent RNA polymerase 6 )
HSP 1 Score: 561.6 bits (1446), Expect = 1.4e-159
Identity = 379/1068 (35.49%), Postives = 562/1068 (52.62%), Query Frame = 0
Query: 98 RMENLTLHLGSQISKEKLSVIWKGENC-----------------SVEFGSGVRKLFVYVN 157
++ +TL +G+ +S++ V W+ E S F + +N
Sbjct: 131 KLAGITLEIGTLVSRDDFFVSWRAEGVDFLVDPFDNTCKFCFRKSTAFSFKDAVMHAVIN 190
Query: 158 LEYKMEVWFESILRVQLRCPPDGASKFLLIQLQGAPRIFKKRSSSSSSSSSSSSSRFYFK 217
+YK+E+ I V+ G L++QL +PR++ + + +
Sbjct: 191 CDYKLELLVRDIQTVRQYKTLHGF--VLILQLASSPRVWYRTADDDIYDTVPG------- 250
Query: 218 ESQSSGSRWIRDVDFTPSSCIGQSFALCLELSPGDQ------LPSFFKTLVGYEEIYGPF 277
+ WIR DFT IG+ + + +SP + L F V E + P
Sbjct: 251 DLLDDDDPWIRTTDFTQVGAIGRCHSYRVLISPRYENKLRTALDYFRMRRVQEERVRWPP 310
Query: 278 NLETGSSFSS-TSNLVPIVTPPQGFDLSYKILFKINALVQHGYLPGPLLDDEFFRLVDPS 337
+ F S+ + +G +S++I+F +N+++ G L + FF L+
Sbjct: 311 RIRNEPCFGEPVSDHFFCIHHKEG--ISFEIMFLVNSVLHRGVFNQFQLTERFFDLL--- 370
Query: 338 RFRPEYIERA-LEKLFNLKECFYEPQNWLKQQYRSYYNSKQLPWKPDISLDDGLVYVHRV 397
R +P+ + A L+ L K ++ LK + +L + S D + + R+
Sbjct: 371 RNQPKDVNIASLKHLCTYKRPVFDAYKRLKLVQEWIQKNPKLLGSHEQSED--ISEIRRL 430
Query: 398 QITPSKVYFRGPEANLSNRVVRQYSDDIDNFIRVSFVDEELDKIRPIDLS--------PR 457
ITP++ Y PE LSNRV+R+Y + F+RV+F+DE + I LS
Sbjct: 431 VITPTRAYCLPPEVELSNRVLRRYKAVAERFLRVTFMDESMQTINSNVLSYFVAPIVKDL 490
Query: 458 TSTANGERTRVYDRILSTLRNGIVIGDKKFEFLAFSASQLRENSVWMFASREGLSAADIR 517
TS++ ++T V+ R+ S L +G + +K+ FLAFSA+QLR+ S W FA +DI+
Sbjct: 491 TSSSFSQKTYVFKRVKSILTDGFKLCGRKYSFLAFSANQLRDRSAWFFAEDGKTRVSDIK 550
Query: 518 EWMGDFRQIRNVAKYAARLGQSFGSSRKTLCVEEHEIEVITDV-EVETNNVTYCFSDGIG 577
WMG F+ +NVAK AAR+G F S+ T+ V HE++ T+V ++E N Y FSDGIG
Sbjct: 551 TWMGKFKD-KNVAKCAARMGLCFSSTYATVDVMPHEVD--TEVPDIERNG--YVFSDGIG 610
Query: 578 KISETLAEKVAERCGL-INHTPSAFQIRYAGYKGVVAIDPKSEK--KLSLRKSMLKYKSL 637
I+ LA++V E+ L ++++P A+QIRYAG+KGVVA P +L+LR SM K+ S
Sbjct: 611 TITPDLADEVMEKLKLDVHYSPCAYQIRYAGFKGVVARWPSKSDGIRLALRDSMKKFFSK 670
Query: 638 DTQLDVLAWSKYLPCFLNRQVINLLSTLGIKDRVFEKKQKEAIYQLDCILKDPSRALEVL 697
T L++ +W+++ P FLNRQ+I LLS LG+ D +F Q+ +Y+L+ IL D A EVL
Sbjct: 671 HTILEICSWTRFQPGFLNRQIITLLSVLGVPDEIFWDMQESMLYKLNRILDDTDVAFEVL 730
Query: 698 ELMSPGEMTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCL 757
+ + + +KP EP L ML + R +L LR KSRIFV GR +MGCL
Sbjct: 731 TASCAEQGNTAAIMLSAGFKPKTEPHLRGMLSSVRIAQLWGLREKSRIFVTSGRWLMGCL 790
Query: 758 DETGTLKYGQVFVQCSVP----------GRSSESNF---IAEGKVVVAKNPCLHPGDMRV 817
DE G L++GQ F+Q S P R E+ + +G V +AKNPCLHPGD+R+
Sbjct: 791 DEAGILEHGQCFIQVSKPSIENCFSKHGSRFKETKTDLEVVKGYVAIAKNPCLHPGDVRI 850
Query: 818 LNAVDVKALHHMVDCVVFPQKGQRPHPNECSGSDLDGDLYFVCWDPELTCIRPVK----P 877
L AVDV LHHM DC++FPQKG RPH NE SGSDLDGDLYFV WD +L I P +
Sbjct: 851 LEAVDVPQLHHMYDCLIFPQKGDRPHTNEASGSDLDGDLYFVAWDQKL--IPPNRKSYPA 910
Query: 878 MSYKPAPTIQLDHDVTIEEVQEYMANYMVNDGLGAIANAHTAFADREPRKAMSAKCIKLA 937
M Y A L V +++ ++ A + N+ LG I NAH ADR AM +C+ LA
Sbjct: 911 MHYDAAEEKSLGRAVNHQDIIDFFARNLANEQLGTICNAHVVHADRSEYGAMDEECLLLA 970
Query: 938 KLFSIAVDFPKTGVPANLPPFLRVHEFPDFMEKLDRPAYESKGVIGKLFRSVKDV-SFNT 997
+L + AVDFPKTG ++P L+ +PDFM K D Y+S ++G+L+R VK+V +
Sbjct: 971 ELAATAVDFPKTGKIVSMPFHLKPKLYPDFMGKEDYQTYKSNKILGRLYRRVKEVYDEDA 1030
Query: 998 NTIETYTREVAAKCYDPDMEIDGFEKYLNEAFDFKTRYDYKLGNLMDYYGIRTEPELVSG 1057
+ + +A YD +EI GFE + EA+ K YD +L L+ Y ++ E E+V+G
Sbjct: 1031 EASSEESTDPSAIPYDAVLEIPGFEDLIPEAWGHKCLYDGQLIGLLGQYKVQKEEEIVTG 1090
Query: 1058 NILRVGKSFDKRTD--MEQISHAMRSLRKEARAWFNEKGSKPRCDDDDNGDEEEE----F 1100
+I + K K+ E++ H+ SL+KE R F E D +N EEE+
Sbjct: 1091 HIWSMPKYTSKKQGELKERLKHSYNSLKKEFRKVFEE-----TIPDHENLSEEEKNILYE 1150
BLAST of MS009042 vs. TAIR 10
Match:
AT2G19930.1 (RNA-dependent RNA polymerase family protein )
HSP 1 Score: 121.3 bits (303), Expect = 4.8e-27
Identity = 134/462 (29.00%), Postives = 204/462 (44.16%), Query Frame = 0
Query: 529 VETNNVTYCFSDGIGKISETLA---------------EKVAERCGLINHTPSAFQIRYAG 588
++ N SDG G ISE LA E + E C F++ Y G
Sbjct: 389 LDKNKKPCIHSDGTGYISEDLARMCPLNIFKGKCLRSESIQEACYQDPPLLIQFRMFYDG 448
Query: 589 Y--KGVVAIDPK-SEKKLSLRKSMLKYKSLDTQLDVLAWSKYLPCF----------LNRQ 648
Y KG ++ K + + +R SM+K S D L + L L++
Sbjct: 449 YAVKGTFLLNKKLCPRTVQVRPSMIKV-SKDPSLSNFSTFNALEVVTTSNPPKRTKLSKN 508
Query: 649 VINLLSTLGIKDRVFEKKQKEAIYQLDCILKDPSRALEVLELMSPGEMTSV-LKEMLLYY 708
++ LLS GI + F + + I + AL ++ GEM +M+L
Sbjct: 509 LVALLSYGGIPNEFFLDILLNTLEESKSIFYNKRAALNA--ALNYGEMDDQNAAQMILVG 568
Query: 709 KPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDETGTLKYGQVFVQCSVPG 768
P EP L L + DL+ ++ V + +MG +D TG LK +V V
Sbjct: 569 IPLDEPHLKNYLSILLKTEKNDLKA-GKLPVTESYYLMGTVDPTGALKEDEVCV------ 628
Query: 769 RSSESNFIAEGKVVVAKNPCLHPGDMRVLNAVDVKALHHMVD----CVVFPQKGQRPHPN 828
ES I+ G+V+V +NP LH GD+ +L A VKAL V V FPQKG R +
Sbjct: 629 -ILESGQIS-GEVLVYRNPGLHFGDIHILKATYVKALEEYVGNSKFAVFFPQKGPRSLGD 688
Query: 829 ECSGSDLDGDLYFVCWDPE-LTCIRPVKP-MSYKP--------APTIQLDHDVTIEEVQE 888
E +G D DGD+YF+ +PE L +P +P +S P AP+ QL + EE+ E
Sbjct: 689 EIAGGDFDGDMYFISRNPELLENFKPSEPWVSLTPPSKSNSGRAPS-QLSPEELEEELFE 748
Query: 889 YM--ANYMVNDGLGAIANAHTAFADR---------EPRKAMSAKCIKLAKLFSIAVDFPK 937
A + ++ +G A++ DR E + M K ++L ++ A+D PK
Sbjct: 749 MFLTAGFHASNVIGIAADSWLTIMDRFLILGDDRAEEKAEMKKKMLELIDIYYDALDAPK 808
BLAST of MS009042 vs. TAIR 10
Match:
AT2G19920.1 (RNA-dependent RNA polymerase family protein )
HSP 1 Score: 119.4 bits (298), Expect = 1.8e-26
Identity = 150/592 (25.34%), Postives = 251/592 (42.40%), Query Frame = 0
Query: 436 YDRILSTLRNGIVIGDKKFEFLAF----SASQLRENS---VWMFASREG----------- 495
Y + +NGI++G ++++F F A + + NS V + R G
Sbjct: 268 YSKYKEIAKNGIMVGLRRYQFFVFKDGGKAEKKKRNSTKQVKCYFIRTGSTASSDMENPY 327
Query: 496 -LSAADIREWMGDFRQIRNV---AKYAARLGQSFGSSRKTLCVEEHEIEV--ITDVE--- 555
LS I E F + + A Y AR ++K L V+ EI V I D+
Sbjct: 328 ILSGMSIHEARMHFMHVHTLPSPANYMARFSLILSKTKK-LEVDMTEITVMQIDDIHCHD 387
Query: 556 ------VETNNVTYCFSDGIGKISETLA---------------EKVAERCGLINHTPSAF 615
++ N SDG G ISE LA + C + P
Sbjct: 388 QSNNDVLDKNGKPRIHSDGTGYISEDLARMCPLNIFKGKSMRSNNIQGTC--VQEPPLLI 447
Query: 616 QIRY----AGYKGVVAIDPK-SEKKLSLRKSMLK-YK-------SLDTQLDVLAWSK-YL 675
QIR + KG+ ++ + + +R SM+K YK S L+V+ S
Sbjct: 448 QIRMFNDGSAVKGIFLLNKNLPPQTVQVRPSMIKVYKDKNLSNFSTFNSLEVVTTSNPPK 507
Query: 676 PCFLNRQVINLLSTLGIKDRVF------EKKQKEAIYQLDCILKDPSRALEVLELMSPGE 735
L++ ++ LLS G+ + F ++K+ I+ K + L + +
Sbjct: 508 RAKLSKNLVALLSYGGVPNDFFLDILLNTLEKKKTIF-----FKVRAAGKAALHYGNMDD 567
Query: 736 MTSVLKEMLLYYKPNKEPFLNMMLRTFRADKLLDLRTKSRIFVPKGRTMMGCLDETGTLK 795
++ +M++ P EP+L L + DL+ ++ + + +MG +D TG LK
Sbjct: 568 KNAL--QMIMAGIPLDEPYLKHYLSKLLKLEKDDLKA-GKLPIDESYYLMGTVDPTGELK 627
Query: 796 YGQVFVQCSVPGRSSESNFIAEGKVVVAKNPCLHPGDMRVLNAVDVKALHHMVD----CV 855
+V S V+V +NP LH GD+ +L A VK+L V V
Sbjct: 628 EDEV------------SGLAKSQDVLVYRNPGLHFGDIHILKATYVKSLEQYVGNSKYGV 687
Query: 856 VFPQKGQRPHPNECSGSDLDGDLYFVCWDPE-LTCIRPVKP-MSYKPAPTI---QLDHDV 915
FPQKG R +E +G D DGD+YF+ +P+ L +P +P +S P I + ++
Sbjct: 688 FFPQKGPRSLGDEIAGGDFDGDMYFISRNPKLLEHYKPSEPWVSSSPRSKIYTGRQPSEL 747
Query: 916 TIEEVQEYMANYMVNDG------LGAIANAHTAFADR---------EPRKAMSAKCIKLA 936
+ E+++E + + G +G A++ A DR + + M K +KL
Sbjct: 748 SPEQLEEELFKIFLKTGFSPSSVIGQAADSWLAIMDRFLTLGDENVKEKAEMKKKMLKLT 807
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022146462.1 | 0.0e+00 | 98.19 | probable RNA-dependent RNA polymerase 1 [Momordica charantia] | [more] |
| XP_038875555.1 | 0.0e+00 | 79.77 | probable RNA-dependent RNA polymerase 1 [Benincasa hispida] | [more] |
| KAG6579193.1 | 0.0e+00 | 78.74 | RNA-dependent RNA polymerase 1, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
| XP_022994004.1 | 0.0e+00 | 78.62 | probable RNA-dependent RNA polymerase 1 [Cucurbita maxima] | [more] |
| XP_022938807.1 | 0.0e+00 | 78.79 | probable RNA-dependent RNA polymerase 1 [Cucurbita moschata] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q9LQV2 | 0.0e+00 | 58.02 | RNA-dependent RNA polymerase 1 OS=Arabidopsis thaliana OX=3702 GN=RDR1 PE=2 SV=1 | [more] |
| Q0DXS3 | 6.3e-266 | 62.23 | Probable RNA-dependent RNA polymerase 1 OS=Oryza sativa subsp. japonica OX=39947... | [more] |
| O82504 | 1.3e-210 | 39.10 | RNA-dependent RNA polymerase 2 OS=Arabidopsis thaliana OX=3702 GN=RDR2 PE=1 SV=1 | [more] |
| Q7XM31 | 3.0e-199 | 38.55 | Probable RNA-dependent RNA polymerase 2 OS=Oryza sativa subsp. japonica OX=39947... | [more] |
| Q9SG02 | 2.0e-158 | 35.49 | RNA-dependent RNA polymerase 6 OS=Arabidopsis thaliana OX=3702 GN=RDR6 PE=1 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1CZM7 | 0.0e+00 | 98.19 | RNA-dependent RNA polymerase OS=Momordica charantia OX=3673 GN=LOC111015673 PE=3... | [more] |
| A0A6J1K1Q3 | 0.0e+00 | 78.62 | RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111489826 PE=3 SV... | [more] |
| A0A6J1FF44 | 0.0e+00 | 78.79 | RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111444909 PE=3 ... | [more] |
| A0A6J1JTB1 | 0.0e+00 | 78.41 | RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111487656 PE=3 SV... | [more] |
| A0A6J1EB47 | 0.0e+00 | 78.09 | RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111430971 PE=3 ... | [more] |
