MC09g_new0438 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC09g_new0438
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Auxin-responsive protein
Location	MC09: 20817042 .. 20819008 (+)
RNA-Seq Expression	MC09g_new0438
Synteny	MC09g_new0438

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACCCTAACGCGTCAGCCCATCAGTTCATTCTCACCCCTTCTGCTCTCCACTCCGTTTACTGTGATGCCAAAGCATCAGATATTGAAGACGATGATGATATCATAGATTTGGGGCTCAGCCTCAGAACTTTGCAGCCAGATCAGGGCTGTTATGGTCCGCCGCCGGCTGCCGCCGCTGCACCGCTGCCACATTGTATGTATGCGGTGGCGGATTATTGTTGCTGCGGAGGAGAAGAGGCGGAAGGGGTGGTTGAGAGTAAGCTGCAGCGGTGGGCGTATGTGAAGGTAAATATGGATGGAGTGATTGTGGGCAGAAAAGTTTGCATCTTTCGACATTCTTCCTATTCTAGTCTTGCTCTTCAGCTTGAAGATATGTTTGGTAATTCATTCATCACTTTTTCCTTTTCCATGCTATTCTTCTTTTTTGTTCAATCAGATGGAGATTTAAATCTTCGATTTCAGAAACATCATTAGCAATACATCAATTTGGGTTATTTTTCTTGATTGGGTCTTGAGAGTATTTGATTTTAAATAAATGAGAGAATTCGAGACCATGATATATAAGATATGTGATCAAGCTAGAAATTTTCTTGAAATCTTACCTTTAAACATATTAACAAATTAGAGTACAAAAATAAAAATCTTTAGTGTGTAATTTTTTATTTTTTGGATTTATGACAAAACTCATTGCCATATCTCTTCGCTTTTTGTTTTGCCAGAGTGAGCTATTATCTTTTTGTTTGTTTTGGATTGTAAAAATTATACATGAATGTTAAAGATTTTAAATTGTTGTTTCTAAATATTTTTAAATTGAAAAGATAATGGATATAGGAAGTTAGGAGGTAAAAATTTACGTCATTTATAAAGAAATTTGACTTTATTTATTAATATATCTAGATGTGTAAGATCATCTCTTTCACAACATTGTATCTCTTCCTATAGAGTTTTGAAACTTTATGTAAGGAGAGTGAAGGCAAAATTAATTTTGGTGGGGAAGAGGGGACTACGATTATAACAAAGAAAGGCAAAAGACAAAAGCCCTTTATGTAGCATTCATCTTATGAAATAAATGAAAAAGGAACTACAAAATAATTATGAAATAATGAAAACAACAAATTATAAACAAGATCCTTTCTTGTTTTATTCTAAATTGTATTAATTAGCACAGCCAAAAAGATCTTCTTGAAAGATTGTCAACATAAGCTACATAGATCATCCATTAACGATGGTTCTTCTCTTATTTATATTTCGAGAAGAAATTGCTCGATTGGTCGATGTCAACAGTATATTAAAATCTTGATTATGTCTTTGTCCTTTAATTTAGATTTTTCCATCAAAATAATGTCTTTATACCTAATCAGAATTCTTATAATTACCATAAACGTCTTTGAGTGCTAAAATAATTGAGTACAAAATGGGCCTCTTGATCATATAGTGAATTGTCATGTTAGAATTTTCTTACTTGTCAATTATACTGGTAAGATTGACTAGTAGAGTTTAATTTGAATTAAATGATTGAGGTGATAATGGAAAATAATAATGTTATAGATTTTTCTATAATTTTTGCATCTCATCCCTCAGTTTGAGAGTAACTCAATGAATAAAGGGAGCAATTATCCTCCCCGTCGTCTTGATTTATATTAAGTAAAACACTCACATCTATGTTCAATGGTCGGATCAGGTCGACAGTGCGAATCTGGATTAAAGTTGTTTGAAACAGATTCGGAATATAATCTATTTTATAAAGGTGGAGACGAAATTTGGAGGAGCGTTGGCGATGTACCATGGAAGTAAGTTCTCTATCGTCATTGTTCATCATATATTTGTTCGGTTGACTCCATCAAACCGTTACATAATTTGTCTGTTTTTTGTTTGCAAATGGACATAGGCAGTTCGTAGAAGGTGTGAAGAGGCTAAGAATTGCTCGAAAAGATGAGGCTTTTGTTGTCATTCACCAAAATTAA

mRNA sequence

ATGGACCCTAACGCGTCAGCCCATCAGTTCATTCTCACCCCTTCTGCTCTCCACTCCGTTTACTGTGATGCCAAAGCATCAGATATTGAAGACGATGATGATATCATAGATTTGGGGCTCAGCCTCAGAACTTTGCAGCCAGATCAGGGCTGTTATGGTCCGCCGCCGGCTGCCGCCGCTGCACCGCTGCCACATTGTATGTATGCGGTGGCGGATTATTGTTGCTGCGGAGGAGAAGAGGCGGAAGGGGTGGTTGAGAGTAAGCTGCAGCGGTGGGCGTATGTGAAGGTAAATATGGATGGAGTGATTGTGGGCAGAAAAGTTTGCATCTTTCGACATTCTTCCTATTCTAGTCTTGCTCTTCAGCTTGAAGATATGTTTGGTCGACAGTGCGAATCTGGATTAAAGTTGTTTGAAACAGATTCGGAATATAATCTATTTTATAAAGGTGGAGACGAAATTTGGAGGAGCGTTGGCGATGTACCATGGAAGCAGTTCGTAGAAGGTGTGAAGAGGCTAAGAATTGCTCGAAAAGATGAGGCTTTTGTTGTCATTCACCAAAATTAA

Coding sequence (CDS)

ATGGACCCTAACGCGTCAGCCCATCAGTTCATTCTCACCCCTTCTGCTCTCCACTCCGTTTACTGTGATGCCAAAGCATCAGATATTGAAGACGATGATGATATCATAGATTTGGGGCTCAGCCTCAGAACTTTGCAGCCAGATCAGGGCTGTTATGGTCCGCCGCCGGCTGCCGCCGCTGCACCGCTGCCACATTGTATGTATGCGGTGGCGGATTATTGTTGCTGCGGAGGAGAAGAGGCGGAAGGGGTGGTTGAGAGTAAGCTGCAGCGGTGGGCGTATGTGAAGGTAAATATGGATGGAGTGATTGTGGGCAGAAAAGTTTGCATCTTTCGACATTCTTCCTATTCTAGTCTTGCTCTTCAGCTTGAAGATATGTTTGGTCGACAGTGCGAATCTGGATTAAAGTTGTTTGAAACAGATTCGGAATATAATCTATTTTATAAAGGTGGAGACGAAATTTGGAGGAGCGTTGGCGATGTACCATGGAAGCAGTTCGTAGAAGGTGTGAAGAGGCTAAGAATTGCTCGAAAAGATGAGGCTTTTGTTGTCATTCACCAAAATTAA

Protein sequence

MDPNASAHQFILTPSALHSVYCDAKASDIEDDDDIIDLGLSLRTLQPDQGCYGPPPAAAAAPLPHCMYAVADYCCCGGEEAEGVVESKLQRWAYVKVNMDGVIVGRKVCIFRHSSYSSLALQLEDMFGRQCESGLKLFETDSEYNLFYKGGDEIWRSVGDVPWKQFVEGVKRLRIARKDEAFVVIHQN

Homology

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BLAST of MC09g_new0438 vs. ExPASy Swiss-Prot
Match: Q8RYC6 (Auxin-responsive protein IAA32 OS=Arabidopsis thaliana OX=3702 GN=IAA32 PE=1 SV=2)

HSP 1 Score: 139.4 bits (350), Expect = 4.1e-32
Identity = 81/197 (41.12%), Postives = 116/197 (58.88%), Query Frame = 0


Query: 1   MDPNASAHQFILTPSALHSVYCDAKASDIEDDDDIIDLGLSLRTLQPDQGCYGPPPAAA- 60
           MDPN  A  F    S  H+ Y   K         +IDLGLSLRT+Q +   Y PP     
Sbjct: 1   MDPNTPA-DFFKGSSKFHTYYSQTKKG-----GGVIDLGLSLRTIQHE--TYLPPARMIG 60

Query: 61  ----------AAPLPHCMYAVADYCCCGGEEAEGVVESKLQ---RWAYVKVNMDGVIVGR 120
                     + P  + +  +          A+G   ++ +   ++AYVKVN+DG++VGR
Sbjct: 61  LDGYGELIDWSQPSYNSITQLKSEDTGHQRLAQGYYNNEGESRGKYAYVKVNLDGLVVGR 120

Query: 121 KVCIFRHSSYSSLALQLEDMFGRQCESGLKLFETDSEYNLFYKGGDEIWRSVGDVPWKQF 180
           KVC+    +Y++LALQL DMFG Q  SGL+LF+T+SE++L Y+  + IWR+VGDVPWK+F
Sbjct: 121 KVCLVDQGAYATLALQLNDMFGMQTVSGLRLFQTESEFSLVYRDREGIWRNVGDVPWKEF 180

Query: 181 VEGVKRLRIARKDEAFV 184
           VE V R+RIAR+++A +
Sbjct: 181 VESVDRMRIARRNDALL 189

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BLAST of MC09g_new0438 vs. ExPASy Swiss-Prot
Match: Q9C5X0 (Auxin-responsive protein IAA34 OS=Arabidopsis thaliana OX=3702 GN=IAA34 PE=1 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 1.7e-30
Identity = 67/147 (45.58%), Postives = 91/147 (61.90%), Query Frame = 0


Query: 35  IIDLGLSLRTLQPDQGCYGPPPAAAAAPLPHCMYAVADYCCCGGEEAEGVVESKLQRWAY 94
           +IDLGLSLRT+Q                  H +Y  +    C         E   ++W Y
Sbjct: 60  VIDLGLSLRTIQ------------------HEIYHSSGQRYCSN-------EGYRRKWGY 119

Query: 95  VKVNMDGVIVGRKVCIFRHSSYSSLALQLEDMFGRQCESGLKLFETDSEYNLFYKGGDEI 154
           VKV MDG++VGRKVC+  H SYS+LA QLEDMFG Q  SGL+LF+ +SE+ L Y+  + +
Sbjct: 120 VKVTMDGLVVGRKVCVLDHGSYSTLAHQLEDMFGMQSVSGLRLFQMESEFCLVYRDEEGL 179

Query: 155 WRSVGDVPWKQFVEGVKRLRIARKDEA 182
           WR+ GDVPW +F+E V+RLRI R+++A
Sbjct: 180 WRNAGDVPWNEFIESVERLRITRRNDA 181

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BLAST of MC09g_new0438 vs. ExPASy Swiss-Prot
Match: A2XB18 (Auxin-responsive protein IAA10 OS=Oryza sativa subsp. indica OX=39946 GN=IAA10 PE=2 SV=2)

HSP 1 Score: 92.0 bits (227), Expect = 7.5e-18
Identity = 51/111 (45.95%), Postives = 66/111 (59.46%), Query Frame = 0


Query: 79  EEAEGVVESKLQRWAYVKVNMDGVIVGRKVCIFRHSSYSSLALQLEDMFGRQ----CES- 138
           ++ EG  + ++  W  VKVNMDG ++GRKV +  H SY +LAL LE MF +     C S 
Sbjct: 152 DKEEGEKKGRVAGW--VKVNMDGEVIGRKVDLNAHRSYKTLALALELMFTKPSIGLCASH 211

Query: 139 ---GLKLFETDSEYNLFYKGGDEIWRSVGDVPWKQFVEGVKRLRIARKDEA 182
               LKL +  +EY L Y+  D  W  VGDVPW+ FV  VKRLRI R  +A
Sbjct: 212 NTNSLKLLDNSAEYQLTYEDRDGDWMLVGDVPWEMFVSSVKRLRIMRTSDA 260

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BLAST of MC09g_new0438 vs. ExPASy Swiss-Prot
Match: Q0DWF2 (Auxin-responsive protein IAA10 OS=Oryza sativa subsp. japonica OX=39947 GN=IAA10 PE=2 SV=2)

HSP 1 Score: 92.0 bits (227), Expect = 7.5e-18
Identity = 51/111 (45.95%), Postives = 66/111 (59.46%), Query Frame = 0


Query: 79  EEAEGVVESKLQRWAYVKVNMDGVIVGRKVCIFRHSSYSSLALQLEDMFGRQ----CES- 138
           ++ EG  + ++  W  VKVNMDG ++GRKV +  H SY +LAL LE MF +     C S 
Sbjct: 152 DKEEGEKKGRVAGW--VKVNMDGEVIGRKVDLNAHRSYKTLALALELMFTKPSIGLCASH 211

Query: 139 ---GLKLFETDSEYNLFYKGGDEIWRSVGDVPWKQFVEGVKRLRIARKDEA 182
               LKL +  +EY L Y+  D  W  VGDVPW+ FV  VKRLRI R  +A
Sbjct: 212 NTNSLKLLDNSAEYQLTYEDRDGDWMLVGDVPWEMFVSSVKRLRIMRTSDA 260

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BLAST of MC09g_new0438 vs. ExPASy Swiss-Prot
Match: Q38830 (Auxin-responsive protein IAA12 OS=Arabidopsis thaliana OX=3702 GN=IAA12 PE=1 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.4e-16
Identity = 48/104 (46.15%), Postives = 62/104 (59.62%), Query Frame = 0


Query: 85  VESKLQRWAYVKVNMDGVIVGRKVCIFRHSSYSSLALQLEDMF----GRQCE---SGLKL 144
           V  K+Q   +VKVNMDGV +GRKV +  HSSY +LA  LE+MF    G  C      L+L
Sbjct: 117 VNPKVQGLGFVKVNMDGVGIGRKVDMRAHSSYENLAQTLEEMFFGMTGTTCREKVKPLRL 176

Query: 145 FETDSEYNLFYKGGDEIWRSVGDVPWKQFVEGVKRLRIARKDEA 182
            +  S++ L Y+  +  W  VGDVPW+ F+  VKRLRI    EA
Sbjct: 177 LDGSSDFVLTYEDKEGDWMLVGDVPWRMFINSVKRLRIMGTSEA 220

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BLAST of MC09g_new0438 vs. NCBI nr
Match: XP_038893192.1 (auxin-responsive protein IAA32-like [Benincasa hispida])

HSP 1 Score: 216 bits (551), Expect = 1.19e-68
Identity = 114/170 (67.06%), Postives = 128/170 (75.29%), Query Frame = 0


Query: 20  VYCDAKASDIEDDDDIIDLGLSLRTL-QPDQGCYGPPPAAAAAPLPHCMYAVADYCCCGG 79
           VYC+ K      ++ IIDLGLSLRTL QP+ G  G           +  +   DY CC  
Sbjct: 13  VYCEGKG-----ENGIIDLGLSLRTLEQPEVGIEGG----------YMNWGGQDYYCCTE 72

Query: 80  EEAEGVVESKLQRWAYVKVNMDGVIVGRKVCIFRHSSYSSLALQLEDMFGRQCESGLKLF 139
           E+AE V   + QRW YVKVNMDGVIVGRKVCIF+H+SYS+LALQLEDMFGRQCESGL+LF
Sbjct: 73  EDAEVVQSKQQQRWDYVKVNMDGVIVGRKVCIFQHASYSTLALQLEDMFGRQCESGLRLF 132

Query: 140 ETDSEYNLFYKGGDEIWRSVGDVPWKQFVEGVKRLRIARKDEAFVVIHQN 188
           E DSE++LFYK GDE WRSVGDVPWKQFVEGVKRLRIARKDEAFV I QN
Sbjct: 133 ENDSEFSLFYKDGDENWRSVGDVPWKQFVEGVKRLRIARKDEAFVAIRQN 167

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BLAST of MC09g_new0438 vs. NCBI nr
Match: XP_004135388.1 (auxin-responsive protein IAA32 [Cucumis sativus] >KGN52109.1 hypothetical protein Csa_008762 [Cucumis sativus])

HSP 1 Score: 211 bits (538), Expect = 1.32e-66
Identity = 116/177 (65.54%), Postives = 134/177 (75.71%), Query Frame = 0


Query: 18  HSVYCDAKASDIEDDDDIIDLGLSLRTL-QPDQGCYGPPPAAAAAPLPHCMYAVADYCCC 77
           +SVYC+ K      ++ IIDLGLSLRTL QP+ G            + +      DY CC
Sbjct: 13  NSVYCEGKG-----ENGIIDLGLSLRTLEQPEMG------------IMNWGGQDQDYYCC 72

Query: 78  GGEE---AEGVVESKLQ--RWAYVKVNMDGVIVGRKVCIFRHSSYSSLALQLEDMFGRQC 137
             +E    + VV+SK Q  +WAYVKVNMDGVIVGRKVCIF+++SYS+LALQLEDMFGRQC
Sbjct: 73  TDQEEDHTDQVVQSKEQHQKWAYVKVNMDGVIVGRKVCIFQNASYSTLALQLEDMFGRQC 132

Query: 138 ESGLKLFETDSEYNLFYKGGDEIWRSVGDVPWKQFVEGVKRLRIARKDEAFVVIHQN 188
           ESGL+LFE DSE++LFYK GDE WRSVGDVPWKQFVEGVKRLRIARKDEAFVVIHQN
Sbjct: 133 ESGLRLFENDSEFSLFYKDGDENWRSVGDVPWKQFVEGVKRLRIARKDEAFVVIHQN 172

[Close]

BLAST of MC09g_new0438 vs. NCBI nr
Match: XP_008446639.1 (PREDICTED: auxin-responsive protein IAA32 [Cucumis melo] >KAA0034586.1 auxin-responsive protein IAA32 [Cucumis melo var. makuwa] >TYK09138.1 auxin-responsive protein IAA32 [Cucumis melo var. makuwa])

HSP 1 Score: 211 bits (538), Expect = 1.56e-66
Identity = 116/177 (65.54%), Postives = 134/177 (75.71%), Query Frame = 0


Query: 18  HSVYCDAKASDIEDDDDIIDLGLSLRTL-QPDQGCYGPPPAAAAAPLPHCMYAVADYCCC 77
           +SVYC+ K      ++ IIDLGLSLRTL QP+ G            + +      DY CC
Sbjct: 13  NSVYCEGKG-----ENGIIDLGLSLRTLEQPEMG------------IMNWGGQDQDYYCC 72

Query: 78  GGEE---AEGVVESKLQ--RWAYVKVNMDGVIVGRKVCIFRHSSYSSLALQLEDMFGRQC 137
             +E    + VV+SK Q  +WAYVKVNMDGVIVGRKVCIF+++SYS+LALQLEDMFGRQC
Sbjct: 73  TDQEEDHTDQVVQSKEQHQKWAYVKVNMDGVIVGRKVCIFQNASYSTLALQLEDMFGRQC 132

Query: 138 ESGLKLFETDSEYNLFYKGGDEIWRSVGDVPWKQFVEGVKRLRIARKDEAFVVIHQN 188
           ESGL+LFE DSE++LFYK GDE WRSVGDVPWKQFVEGVKRLRIARKDEAFVVIHQN
Sbjct: 133 ESGLRLFENDSEFSLFYKDGDENWRSVGDVPWKQFVEGVKRLRIARKDEAFVVIHQN 172

[Close]

BLAST of MC09g_new0438 vs. NCBI nr
Match: XP_023513171.1 (auxin-responsive protein IAA32-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 211 bits (538), Expect = 3.09e-66
Identity = 123/197 (62.44%), Postives = 135/197 (68.53%), Query Frame = 0


Query: 19  SVYCDAKASDIEDDDDIIDLGLSLRTLQPD-----QGC---------YGPPPAAAA---- 78
           SVYC+ K      ++  IDLGLSLRTLQP+     +G          +G     AA    
Sbjct: 7   SVYCEGKG-----ENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHGINWGAADHTAADLYM 66

Query: 79  ---APLPHCMYAVA----DYCCCGGEE--AEGVVESKLQRWAYVKVNMDGVIVGRKVCIF 138
                + H    V     DY CC  EE  AE VV+SK QRWAYVKVNMDGVIVGRKVCIF
Sbjct: 67  NINMNMKHNNNGVVVVEQDYYCCEEEEEEAEEVVQSKHQRWAYVKVNMDGVIVGRKVCIF 126

Query: 139 RHSSYSSLALQLEDMFGRQCESGLKLFETDSEYNLFYKGGDEIWRSVGDVPWKQFVEGVK 188
           +H SYSSLALQLEDMFGRQCESGLKLFE +SE++LFYK GDE WRSVGDVPWKQFVE VK
Sbjct: 127 QHGSYSSLALQLEDMFGRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQFVEDVK 186

[Close]

BLAST of MC09g_new0438 vs. NCBI nr
Match: KAG6601273.1 (Auxin-responsive protein IAA32, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 211 bits (538), Expect = 3.30e-66
Identity = 122/199 (61.31%), Postives = 135/199 (67.84%), Query Frame = 0


Query: 19  SVYCDAKASDIEDDDDIIDLGLSLRTLQPD-----QGC---------YGPPPAAAA---- 78
           SVYC+ K      ++  IDLGLSLRTLQP+     +G          +G     AA    
Sbjct: 7   SVYCEGKG-----ENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHGINWGAADHTAADLYM 66

Query: 79  ---APLPHC------MYAVADYCCCGGEE--AEGVVESKLQRWAYVKVNMDGVIVGRKVC 138
                + H       +    DY CC  EE  AE VV+SK QRWAYVKVNMDGVIVGRKVC
Sbjct: 67  NINMNMKHSNNNNGVVVVQQDYYCCAEEEEEAEEVVQSKQQRWAYVKVNMDGVIVGRKVC 126

Query: 139 IFRHSSYSSLALQLEDMFGRQCESGLKLFETDSEYNLFYKGGDEIWRSVGDVPWKQFVEG 188
           IF+H SYSSLALQLEDMFGRQCESGLKLFE +SE++LFYK GDE WRSVGDVPWKQFVE 
Sbjct: 127 IFQHGSYSSLALQLEDMFGRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQFVED 186

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BLAST of MC09g_new0438 vs. ExPASy TrEMBL
Match: A0A0A0KT91 (Auxin-responsive protein OS=Cucumis sativus OX=3659 GN=Csa_5G610430 PE=3 SV=1)

HSP 1 Score: 211 bits (538), Expect = 6.41e-67
Identity = 116/177 (65.54%), Postives = 134/177 (75.71%), Query Frame = 0


Query: 18  HSVYCDAKASDIEDDDDIIDLGLSLRTL-QPDQGCYGPPPAAAAAPLPHCMYAVADYCCC 77
           +SVYC+ K      ++ IIDLGLSLRTL QP+ G            + +      DY CC
Sbjct: 13  NSVYCEGKG-----ENGIIDLGLSLRTLEQPEMG------------IMNWGGQDQDYYCC 72

Query: 78  GGEE---AEGVVESKLQ--RWAYVKVNMDGVIVGRKVCIFRHSSYSSLALQLEDMFGRQC 137
             +E    + VV+SK Q  +WAYVKVNMDGVIVGRKVCIF+++SYS+LALQLEDMFGRQC
Sbjct: 73  TDQEEDHTDQVVQSKEQHQKWAYVKVNMDGVIVGRKVCIFQNASYSTLALQLEDMFGRQC 132

Query: 138 ESGLKLFETDSEYNLFYKGGDEIWRSVGDVPWKQFVEGVKRLRIARKDEAFVVIHQN 188
           ESGL+LFE DSE++LFYK GDE WRSVGDVPWKQFVEGVKRLRIARKDEAFVVIHQN
Sbjct: 133 ESGLRLFENDSEFSLFYKDGDENWRSVGDVPWKQFVEGVKRLRIARKDEAFVVIHQN 172

[Close]

BLAST of MC09g_new0438 vs. ExPASy TrEMBL
Match: A0A5D3CD62 (Auxin-responsive protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G001290 PE=3 SV=1)

HSP 1 Score: 211 bits (538), Expect = 7.55e-67
Identity = 116/177 (65.54%), Postives = 134/177 (75.71%), Query Frame = 0


Query: 18  HSVYCDAKASDIEDDDDIIDLGLSLRTL-QPDQGCYGPPPAAAAAPLPHCMYAVADYCCC 77
           +SVYC+ K      ++ IIDLGLSLRTL QP+ G            + +      DY CC
Sbjct: 13  NSVYCEGKG-----ENGIIDLGLSLRTLEQPEMG------------IMNWGGQDQDYYCC 72

Query: 78  GGEE---AEGVVESKLQ--RWAYVKVNMDGVIVGRKVCIFRHSSYSSLALQLEDMFGRQC 137
             +E    + VV+SK Q  +WAYVKVNMDGVIVGRKVCIF+++SYS+LALQLEDMFGRQC
Sbjct: 73  TDQEEDHTDQVVQSKEQHQKWAYVKVNMDGVIVGRKVCIFQNASYSTLALQLEDMFGRQC 132

Query: 138 ESGLKLFETDSEYNLFYKGGDEIWRSVGDVPWKQFVEGVKRLRIARKDEAFVVIHQN 188
           ESGL+LFE DSE++LFYK GDE WRSVGDVPWKQFVEGVKRLRIARKDEAFVVIHQN
Sbjct: 133 ESGLRLFENDSEFSLFYKDGDENWRSVGDVPWKQFVEGVKRLRIARKDEAFVVIHQN 172

[Close]

BLAST of MC09g_new0438 vs. ExPASy TrEMBL
Match: A0A1S3BG83 (Auxin-responsive protein OS=Cucumis melo OX=3656 GN=LOC103489310 PE=3 SV=1)

HSP 1 Score: 211 bits (538), Expect = 7.55e-67
Identity = 116/177 (65.54%), Postives = 134/177 (75.71%), Query Frame = 0


Query: 18  HSVYCDAKASDIEDDDDIIDLGLSLRTL-QPDQGCYGPPPAAAAAPLPHCMYAVADYCCC 77
           +SVYC+ K      ++ IIDLGLSLRTL QP+ G            + +      DY CC
Sbjct: 13  NSVYCEGKG-----ENGIIDLGLSLRTLEQPEMG------------IMNWGGQDQDYYCC 72

Query: 78  GGEE---AEGVVESKLQ--RWAYVKVNMDGVIVGRKVCIFRHSSYSSLALQLEDMFGRQC 137
             +E    + VV+SK Q  +WAYVKVNMDGVIVGRKVCIF+++SYS+LALQLEDMFGRQC
Sbjct: 73  TDQEEDHTDQVVQSKEQHQKWAYVKVNMDGVIVGRKVCIFQNASYSTLALQLEDMFGRQC 132

Query: 138 ESGLKLFETDSEYNLFYKGGDEIWRSVGDVPWKQFVEGVKRLRIARKDEAFVVIHQN 188
           ESGL+LFE DSE++LFYK GDE WRSVGDVPWKQFVEGVKRLRIARKDEAFVVIHQN
Sbjct: 133 ESGLRLFENDSEFSLFYKDGDENWRSVGDVPWKQFVEGVKRLRIARKDEAFVVIHQN 172

[Close]

BLAST of MC09g_new0438 vs. ExPASy TrEMBL
Match: A0A6J1J6Y8 (Auxin-responsive protein OS=Cucurbita maxima OX=3661 GN=LOC111483950 PE=3 SV=1)

HSP 1 Score: 211 bits (537), Expect = 2.34e-66
Identity = 124/200 (62.00%), Postives = 136/200 (68.00%), Query Frame = 0


Query: 19  SVYCDAKASDIEDDDDIIDLGLSLRTLQPD----------QGCYGPPPAAA---AAPLPH 78
           SVYC+ K      ++  IDLGLSLRTLQP+          +  +G    AA   AA L  
Sbjct: 7   SVYCEGKG-----ENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHGMNWGAADHTAADLYM 66

Query: 79  CM--------------YAVAD---YCCCGGEEAEGVVESKLQRWAYVKVNMDGVIVGRKV 138
            M                V +   YCC   EEAE VV+SK QRWAYVKVNMDGVIVGRKV
Sbjct: 67  NMNININMKHSNNNNGVVVVEQDYYCCEEEEEAEEVVQSKQQRWAYVKVNMDGVIVGRKV 126

Query: 139 CIFRHSSYSSLALQLEDMFGRQCESGLKLFETDSEYNLFYKGGDEIWRSVGDVPWKQFVE 188
           CIF+H SYSSLALQLEDMFGRQCESGLKLFE +SE++LFYK GDE WRSVGDVPWKQFVE
Sbjct: 127 CIFQHGSYSSLALQLEDMFGRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQFVE 186

[Close]

BLAST of MC09g_new0438 vs. ExPASy TrEMBL
Match: A0A6J1GYJ4 (Auxin-responsive protein OS=Cucurbita moschata OX=3662 GN=LOC111458660 PE=3 SV=1)

HSP 1 Score: 210 bits (535), Expect = 4.41e-66
Identity = 121/199 (60.80%), Postives = 134/199 (67.34%), Query Frame = 0


Query: 19  SVYCDAKASDIEDDDDIIDLGLSLRTLQPD-----QGCYGPPPA-----AAAAPLPHCMY 78
           SVYC+ K      ++  IDLGLSLRTLQP+     +G YG          AA      +Y
Sbjct: 7   SVYCEGKG-----ENGSIDLGLSLRTLQPEVVGSIEG-YGEEDEHGINWGAADHTAADLY 66

Query: 79  AVAD-------------------YCCCGGEEAEGVVESKLQRWAYVKVNMDGVIVGRKVC 138
              +                   YCC   EEAE VV+SK QRWAYVKVNMDGVIVGRKVC
Sbjct: 67  MNININMKHSNNNNGVVVVEQDYYCCEEEEEAEEVVQSKQQRWAYVKVNMDGVIVGRKVC 126

Query: 139 IFRHSSYSSLALQLEDMFGRQCESGLKLFETDSEYNLFYKGGDEIWRSVGDVPWKQFVEG 188
           IF+H SYS+LALQLEDMFGRQCESGLKLFE +SE++LFYK GDE WRSVGDVPWKQFVE 
Sbjct: 127 IFQHGSYSNLALQLEDMFGRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQFVED 186

[Close]

BLAST of MC09g_new0438 vs. TAIR 10
Match: AT2G01200.2 (indole-3-acetic acid inducible 32 )

HSP 1 Score: 139.4 bits (350), Expect = 2.9e-33
Identity = 81/197 (41.12%), Postives = 116/197 (58.88%), Query Frame = 0


Query: 1   MDPNASAHQFILTPSALHSVYCDAKASDIEDDDDIIDLGLSLRTLQPDQGCYGPPPAAA- 60
           MDPN  A  F    S  H+ Y   K         +IDLGLSLRT+Q +   Y PP     
Sbjct: 1   MDPNTPA-DFFKGSSKFHTYYSQTKKG-----GGVIDLGLSLRTIQHE--TYLPPARMIG 60

Query: 61  ----------AAPLPHCMYAVADYCCCGGEEAEGVVESKLQ---RWAYVKVNMDGVIVGR 120
                     + P  + +  +          A+G   ++ +   ++AYVKVN+DG++VGR
Sbjct: 61  LDGYGELIDWSQPSYNSITQLKSEDTGHQRLAQGYYNNEGESRGKYAYVKVNLDGLVVGR 120

Query: 121 KVCIFRHSSYSSLALQLEDMFGRQCESGLKLFETDSEYNLFYKGGDEIWRSVGDVPWKQF 180
           KVC+    +Y++LALQL DMFG Q  SGL+LF+T+SE++L Y+  + IWR+VGDVPWK+F
Sbjct: 121 KVCLVDQGAYATLALQLNDMFGMQTVSGLRLFQTESEFSLVYRDREGIWRNVGDVPWKEF 180

Query: 181 VEGVKRLRIARKDEAFV 184
           VE V R+RIAR+++A +
Sbjct: 181 VESVDRMRIARRNDALL 189

[Close]

BLAST of MC09g_new0438 vs. TAIR 10
Match: AT1G15050.1 (indole-3-acetic acid inducible 34 )

HSP 1 Score: 134.0 bits (336), Expect = 1.2e-31
Identity = 67/147 (45.58%), Postives = 91/147 (61.90%), Query Frame = 0


Query: 35  IIDLGLSLRTLQPDQGCYGPPPAAAAAPLPHCMYAVADYCCCGGEEAEGVVESKLQRWAY 94
           +IDLGLSLRT+Q                  H +Y  +    C         E   ++W Y
Sbjct: 60  VIDLGLSLRTIQ------------------HEIYHSSGQRYCSN-------EGYRRKWGY 119

Query: 95  VKVNMDGVIVGRKVCIFRHSSYSSLALQLEDMFGRQCESGLKLFETDSEYNLFYKGGDEI 154
           VKV MDG++VGRKVC+  H SYS+LA QLEDMFG Q  SGL+LF+ +SE+ L Y+  + +
Sbjct: 120 VKVTMDGLVVGRKVCVLDHGSYSTLAHQLEDMFGMQSVSGLRLFQMESEFCLVYRDEEGL 179

Query: 155 WRSVGDVPWKQFVEGVKRLRIARKDEA 182
           WR+ GDVPW +F+E V+RLRI R+++A
Sbjct: 180 WRNAGDVPWNEFIESVERLRITRRNDA 181

[Close]

BLAST of MC09g_new0438 vs. TAIR 10
Match: AT1G04550.2 (AUX/IAA transcriptional regulator family protein )

HSP 1 Score: 87.8 bits (216), Expect = 1.0e-17
Identity = 48/104 (46.15%), Postives = 62/104 (59.62%), Query Frame = 0


Query: 85  VESKLQRWAYVKVNMDGVIVGRKVCIFRHSSYSSLALQLEDMF----GRQCE---SGLKL 144
           V  K+Q   +VKVNMDGV +GRKV +  HSSY +LA  LE+MF    G  C      L+L
Sbjct: 117 VNPKVQGLGFVKVNMDGVGIGRKVDMRAHSSYENLAQTLEEMFFGMTGTTCREKVKPLRL 176

Query: 145 FETDSEYNLFYKGGDEIWRSVGDVPWKQFVEGVKRLRIARKDEA 182
            +  S++ L Y+  +  W  VGDVPW+ F+  VKRLRI    EA
Sbjct: 177 LDGSSDFVLTYEDKEGDWMLVGDVPWRMFINSVKRLRIMGTSEA 220

[Close]

BLAST of MC09g_new0438 vs. TAIR 10
Match: AT2G33310.1 (auxin-induced protein 13 )

HSP 1 Score: 87.8 bits (216), Expect = 1.0e-17
Identity = 45/98 (45.92%), Postives = 59/98 (60.20%), Query Frame = 0


Query: 94  YVKVNMDGVIVGRKVCIFRHSSYSSLALQLEDMFGR----------QCESGLKLFETDSE 153
           ++KVNMDGV +GRKV +  HSSY +LA  LEDMF R          Q    L+L +  SE
Sbjct: 130 FIKVNMDGVAIGRKVDLNAHSSYENLAQTLEDMFFRTNPGTVGLTSQFTKPLRLLDGSSE 189

Query: 154 YNLFYKGGDEIWRSVGDVPWKQFVEGVKRLRIARKDEA 182
           + L Y+  +  W  VGDVPW+ F+  VKRLR+ +  EA
Sbjct: 190 FVLTYEDKEGDWMLVGDVPWRMFINSVKRLRVMKTSEA 227

[Close]

BLAST of MC09g_new0438 vs. TAIR 10
Match: AT2G33310.2 (auxin-induced protein 13 )

HSP 1 Score: 87.8 bits (216), Expect = 1.0e-17
Identity = 45/98 (45.92%), Postives = 59/98 (60.20%), Query Frame = 0


Query: 94  YVKVNMDGVIVGRKVCIFRHSSYSSLALQLEDMFGR----------QCESGLKLFETDSE 153
           ++KVNMDGV +GRKV +  HSSY +LA  LEDMF R          Q    L+L +  SE
Sbjct: 131 FIKVNMDGVAIGRKVDLNAHSSYENLAQTLEDMFFRTNPGTVGLTSQFTKPLRLLDGSSE 190

Query: 154 YNLFYKGGDEIWRSVGDVPWKQFVEGVKRLRIARKDEA 182
           + L Y+  +  W  VGDVPW+ F+  VKRLR+ +  EA
Sbjct: 191 FVLTYEDKEGDWMLVGDVPWRMFINSVKRLRVMKTSEA 228

The following BLAST results are available for this feature:

BLAST of MC09g_new0438 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RYC6	4.1e-32	41.12	Auxin-responsive protein IAA32 OS=Arabidopsis thaliana OX=3702 GN=IAA32 PE=1 SV=...	[more]
Q9C5X0	1.7e-30	45.58	Auxin-responsive protein IAA34 OS=Arabidopsis thaliana OX=3702 GN=IAA34 PE=1 SV=...	[more]
A2XB18	7.5e-18	45.95	Auxin-responsive protein IAA10 OS=Oryza sativa subsp. indica OX=39946 GN=IAA10 P...	[more]
Q0DWF2	7.5e-18	45.95	Auxin-responsive protein IAA10 OS=Oryza sativa subsp. japonica OX=39947 GN=IAA10...	[more]
Q38830	1.4e-16	46.15	Auxin-responsive protein IAA12 OS=Arabidopsis thaliana OX=3702 GN=IAA12 PE=1 SV=...	[more]

BLAST of MC09g_new0438 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038893192.1	1.19e-68	67.06	auxin-responsive protein IAA32-like [Benincasa hispida]	[more]
XP_004135388.1	1.32e-66	65.54	auxin-responsive protein IAA32 [Cucumis sativus] >KGN52109.1 hypothetical protei...	[more]
XP_008446639.1	1.56e-66	65.54	PREDICTED: auxin-responsive protein IAA32 [Cucumis melo] >KAA0034586.1 auxin-res...	[more]
XP_023513171.1	3.09e-66	62.44	auxin-responsive protein IAA32-like isoform X2 [Cucurbita pepo subsp. pepo]	[more]
KAG6601273.1	3.30e-66	61.31	Auxin-responsive protein IAA32, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of MC09g_new0438 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KT91	6.41e-67	65.54	Auxin-responsive protein OS=Cucumis sativus OX=3659 GN=Csa_5G610430 PE=3 SV=1	[more]
A0A5D3CD62	7.55e-67	65.54	Auxin-responsive protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A1S3BG83	7.55e-67	65.54	Auxin-responsive protein OS=Cucumis melo OX=3656 GN=LOC103489310 PE=3 SV=1	[more]
A0A6J1J6Y8	2.34e-66	62.00	Auxin-responsive protein OS=Cucurbita maxima OX=3661 GN=LOC111483950 PE=3 SV=1	[more]
A0A6J1GYJ4	4.41e-66	60.80	Auxin-responsive protein OS=Cucurbita moschata OX=3662 GN=LOC111458660 PE=3 SV=1	[more]

BLAST of MC09g_new0438 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G01200.2	2.9e-33	41.12	indole-3-acetic acid inducible 32	[more]
AT1G15050.1	1.2e-31	45.58	indole-3-acetic acid inducible 34	[more]
AT1G04550.2	1.0e-17	46.15	AUX/IAA transcriptional regulator family protein	[more]
AT2G33310.1	1.0e-17	45.92	auxin-induced protein 13	[more]
AT2G33310.2	1.0e-17	45.92	auxin-induced protein 13	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR033389	AUX/IAA domain	PFAM	PF02309	AUX_IAA	coord: 85..182 e-value: 3.6E-21 score: 76.1
None	No IPR available	GENE3D	3.10.20.90		coord: 87..186 e-value: 8.2E-26 score: 91.9
None	No IPR available	PANTHER	PTHR31734:SF114	AUXIN-RESPONSIVE PROTEIN IAA34	coord: 6..183
None	No IPR available	SUPERFAMILY	54277	CAD & PB1 domains	coord: 87..175
IPR003311	AUX/IAA protein	PANTHER	PTHR31734	AUXIN-RESPONSIVE PROTEIN IAA17	coord: 6..183
IPR000270	PB1 domain	PROSITE	PS51745	PB1	coord: 92..180 score: 17.424492

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC09g_new0438.1	MC09g_new0438.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009734	auxin-activated signaling pathway
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005515	protein binding