IVF0013677 (gene) Melon (IVF77) v1

Overview
NameIVF0013677
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionPre-mRNA-splicing factor 18
Locationchr01: 27493417 .. 27497495 (-)
RNA-Seq ExpressionIVF0013677
SyntenyIVF0013677
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCATTTTTCTTTCCCCTTTCTCTTCCAATTCAGAGCTCCGGCACTAAGCCCTAACAGGATTCCGGCGACCGAGAAGCCGAAACGGAATCAATGGATCTTCTCAAGCAAGAACTCCTAAAGAAACGTCAAGCCCTAGCCACCGAAACCGGCGGCAAGCGCGTTTTCAAGCGATCCGAAATCGAACAGAAGCAAATCCAACAGCTACGCGAGAAAGAGAAGCGCGAATGGAAGCTAAAGCTCTCCGCCAATCCACCGCCTCCGCTACCGCCGCTGAGGCCGCCTCCACATCTTCCAAATCCAATCCATTAGCTTCAGCCTCCACCACTTCCTCCTCCGTCGCAATCAGCTCTACCACCACCACTTCGAAGTCCCTAACGGACGAGCAGAATATCGACAACCTCGTCCTTCCCAGACAGGAAGTGATTCGAAGACTCCGGTTTCTGAAACAACCCATTACGCTTTTCGGGGAAGATGATGATGCTCGGCTTGACCGCTTGAAGTTCGTGCTGAAAGCGGGGCTTTTCGAGGTGGATAGTGACTTGACTGAGGGGCAAACGAATGACTTTCTTCGGGATATTGCAGAGTTGAGGAAGCGGCAGAAGACGGGGATTTTGAGCGAGCGGAAGAGGCAGAAGAAGGAGGATGGTGCTGGGGAAGAGGGAGATGGAGGTGGGGCCGATGAAGAGAGTGCCGATGGAGGGTCGAGTGGGGTTGAGACTGACAAAGATTTAAAGAGAATGAAAGCTAATTTTGATGAGTTATGTGAGGAAGATAAGATATTGGTGTTTTTTAAGAGGTTGTTAAACGAGTGGAACCAGGAATTGGATGAAATGCCTGAAGCAGAGAAGCGGACGGCCAAAGGGAAGTCTATGGTAGCAACATTCAAGCAGTGTGCTCGTTATTTACATCCTCTGTTCAAGTTTTGCAGGAAGAAGGTACGATTTTTGTCCCTTTAGTTACCTAGTCGATTTGTTGAACGTTCAATTCTCTTGATGATTCTTCATCTTGTCATCCTTAGACGTCATGGTAGGTTCGAGAATAACCAGATTGTCTTTTTCTATGTTAGTGGCTTTAAATAAAGAAGGAAAGTTAGTCCCAAGTTAGAGATGTTTACAAATAGCCCTTCCAAGTGGCTAAGAGAGTACTAAATAGAAGAGTTAAAAGCAGCTTAAAATTTACACCAAGCTAAAGCCACAAGAATAAGAAAATCCAAAAGATTGCTTAAGGACTTACTTTTTTTGTTGAATGTTAATATTCATCTCTAGCCATAGTAGTCAAAAGTGAGTTCTAGCAAAATCTGTACCCAAATTCCAAATAACTTTCTCTCTTTTTGAAACGATGACTATCAAGTATCCAAGGGAGTAGGTTGTTAACGTTAAAGGGAGAATGACTTGGGACTAGAAGTTCGTTTGCATATAATTCGGCAGTTCCTCAGCCAACTGGCACATGAGGAAGAGGTGACTTTTGGATTCGCATCTTCCAATACTTAGGTCTAGGGCATGGCATCAATTTGGAAATCTTTTTTCTTTTTTTGTTAGTCTTGGTGCTCCCATGAGAGAGCTTCTACAAGAAAACCTCAACTTCCTTTGGATATATTTCAAGTGGCATATCACCCTTGTAAAGATTGGTTTTCTTTGGAATTATTTGTCCACCATATCCTTGAATAGGAAGTAGGAAAGAGAAAAAAAATGTATTTTTCAGACAGCTGAATTCACTTATTGGAAGAGCACAAGTACAAGTTGGAGAGCAAGTATGGGAAAGAGACCCATTCTTTGTTTTCAGATCCAAAAAAAAAAAAAACCTTCAAACCAGGTCCTAGTTTCAAGTAGAGAGGTTCTAAATGTGTCTTGGATAGGAGCAATTTAACAATTGTAGATAGAGGATCTCCTTGGGAAAGAATTTGAAAATGAAGCTTGTAGGAGTGGTCATTCGACATTTTGATCCTATTGGAGTTCAAATCTTTTTAATCGATAATAGGTAAACGATTCAACAATCGCCAATAAAACATAGCTCAAGTGGCTATCAATCTCGATGTCTGAAGTTCAATCCCATCCCCCACCCCACATATGGTTAAAAAAAAAAAAGTAACCATTTGGCAATCACCTTTGGAAGTGTGGTAAGAAAATAGATACTGATAATAAACCATTATTTCATTTGTCAAGTCAGATTTCTTATGATCACAATGTTGGACTATGTCCACCTGTTTACGTAGTTGGTTGCTGGCAGTTTTGTTATTACCAGGGCTGCCGGCTGAATGCTTAACTTATGGCCTGATAGCGTCTTCAGTTAGACCCTTGTCTCTAGCCACCTGTGTGAGAGTAACTGTCCCCTTCTTGTCGCCACCCTATGAATCATATATTACTAGTTTAGAACTATCTAACCCCATTGGTGTGGTGGGCCGTCATTGTTAATTTCAATTGCACTAATTGACGTACCAAGCTCTCCTTCCCCTCTCCTAGGGATGGCTGTGGAATGTACATTACATTTTTTGTTTTCATATAATTGAATAAAGTGCTGTTTTCTGTATTTTGCCATATGCAAGTGTCATTCTCAAACTTCCCTCTCCAAATCTACTATTATATACTTATTCTTTTTTTTTTTTTTTATGTTCATTGCAAATCACTACATCAGTTGACCAATGTTATGTACGCGACAAACATATACTTAAATAGAAGTTTTTTATTTCTAATTATTTATCTGAGGGTTCTTCGGGATAAAAATTCTGGCATGTATCATGTTTGTATGGAATGAATTATTGCATGTATCAGGTAACTTCACATGAGTTTTATGTTTAGTGCTCTTTGTGGCTTGCAGCTTTGCAATGTCTTTAGATATAAATGTATAACTTGACATTTGTGAGTGCTGAGAAACAAGAGAACCGTGATGCAAGTTTGCTGAAATCAATTGATTATCTTGTAATATTTTCTTTTTAGCAGATAATTTTCATTAATGTATTATATTATTTTCTTTTAGGTTCTTCCCGATGATATTCGGCAAGCATTGTTGGTAGTTGTTGAATGCTGCATGAAGCGAGACTATCTAGCTGCCATGGATCAATACATTAAGATGGCCATTGGAAATGCCCCATGGCCTATTGGTGTCACTATGGTTGGTATTCACGAACGTTCAGCTCGTGAAAAGATTTACACCAACAGTGTGGCTCATATCATGAATGATGAAACAACAAGGAAATACCTGCAATCAATCAAGAGATTGATGACGTTCTGTCAACGGCGCTATCCAACGATGCCATCCAAGGCAGTAGAATTTAACAGCTTGGCAAACGGTAGTGACCTGCAATCTCTGCTAGCAGAAGAGAGAGTTTCTGGAGGAGGCAAGCTGGGTTCTGATGAAAGGCTACGAATCATGCCTGCCCCGGAAGATAGCTAGGAAGTTAAACTATCCTCTCTTGGCTCATCTTCTTTACCGTCTTCATATATGTAATATATAAAGTTCTTCAGCATAGTTTGGTAATCATTGAGTTGATTTTGGATTACTGTTTGAGATTGAAGTCTGTCGACGTTGCTAGCCAGCGATGTTTTGTCTACAGCTTTGCAAACGGTAATGATGCAGTCCATGCTATCTGAAGAGCTTTTGGGGGAATCAATCAGGGTGTCTCGAGGAAGTGCTTCGAATCATGCCCATCCCGAAGACCTGGTAAGAAGATCATCTCCTCGCTGCATTCTTTATGTCTGTATGATAAGTAATTTGAATCTAGCTTGGTAGTCAGTAAGTCAATGGCCAAGTACTGAACAAGATTTTTCTGTTTCTTCCAACTTCGACAATACGTGGAGTGAGAGATGATCTCAAGCTTTTTGGTCCAAAGGTTATATACTTTGTATCTCTCGCTTCTTTTGAGTAAGGATTGAGATTGAAGTATTTACCTAATATTTTGTTGTTTTGCTATGGTGGGTGATTGAGTGCAATCTCAAGCTTATTGAGTAGGGTGACAAGTGTATTAGGAATAAATAAAAGACAATGTCATAGTAGTGGATTCTCAATTGAAAGGTTTTTTTCTTTTCTTTTATTTTTTCAATCTACCGTTCTTTTTACAATATAATTTAATTACTCAAAAGGG

mRNA sequence

CTCATTTTTCTTTCCCCTTTCTCTTCCAATTCAGAGCTCCGGCACTAAGCCCTAACAGGATTCCGGCGACCGAGAAGCCGAAACGGAATCAATGGATCTTCTCAAGCAAGAACTCCTAAAGAAACGTCAAGCCCTAGCCACCGAAACCGGCGGCAAGCGCGTTTTCAAGCGATCCGAAATCGAACAGAAGCAAATCCAACAGCTACGCGAGAAAGAGAAGCGCGAATGGAAGCTAAAGCTCTCCGCCAATCCACCGCCTCCGCTACCGCCGCTGAGGCCGCCTCCACATCTTCCAAATCCAATCCATTAGCTTCAGCCTCCACCACTTCCTCCTCCGTCGCAATCAGCTCTACCACCACCACTTCGAAGTCCCTAACGGACGAGCAGAATATCGACAACCTCGTCCTTCCCAGACAGGAAGTGATTCGAAGACTCCGGTTTCTGAAACAACCCATTACGCTTTTCGGGGAAGATGATGATGCTCGGCTTGACCGCTTGAAGTTCGTGCTGAAAGCGGGGCTTTTCGAGGTGGATAGTGACTTGACTGAGGGGCAAACGAATGACTTTCTTCGGGATATTGCAGAGTTGAGGAAGCGGCAGAAGACGGGGATTTTGAGCGAGCGGAAGAGGCAGAAGAAGGAGGATGGTGCTGGGGAAGAGGGAGATGGAGGTGGGGCCGATGAAGAGAGTGCCGATGGAGGGTCGAGTGGGGTTGAGACTGACAAAGATTTAAAGAGAATGAAAGCTAATTTTGATGAGTTATGTGAGGAAGATAAGATATTGGTGTTTTTTAAGAGGTTGTTAAACGAGTGGAACCAGGAATTGGATGAAATGCCTGAAGCAGAGAAGCGGACGGCCAAAGGGAAGTCTATGGTAGCAACATTCAAGCAGTGTGCTCGTTATTTACATCCTCTGTTCAAGTTTTGCAGGAAGAAGGTTCTTCCCGATGATATTCGGCAAGCATTGTTGGTAGTTGTTGAATGCTGCATGAAGCGAGACTATCTAGCTGCCATGGATCAATACATTAAGATGGCCATTGGAAATGCCCCATGGCCTATTGGTGTCACTATGGTTGGTATTCACGAACGTTCAGCTCGTGAAAAGATTTACACCAACAGTGTGGCTCATATCATGAATGATGAAACAACAAGGAAATACCTGCAATCAATCAAGAGATTGATGACGTTCTGTCAACGGCGCTATCCAACGATGCCATCCAAGGCAGTAGAATTTAACAGCTTGGCAAACGGTAGTGACCTGCAATCTCTGCTAGCAGAAGAGAGAGTTTCTGGAGGAGGCAAGCTGGGTTCTGATGAAAGGCTACGAATCATGCCTGCCCCGGAAGATAGCTAGGAAGTTAAACTATCCTCTCTTGGCTCATCTTCTTTACCGTCTTCATATATGTAATATATAAAGTTCTTCAGCATAGTTTGGTAATCATTGAGTTGATTTTGGATTACTGTTTGAGATTGAAGTCTGTCGACGTTGCTAGCCAGCGATGTTTTGTCTACAGCTTTGCAAACGGTAATGATGCAGTCCATGCTATCTGAAGAGCTTTTGGGGGAATCAATCAGGGTGTCTCGAGGAAGTGCTTCGAATCATGCCCATCCCGAAGACCTGGTAAGAAGATCATCTCCTCGCTGCATTCTTTATGTCTGTATGATAAGTAATTTGAATCTAGCTTGGTAGTCAGTAAGTCAATGGCCAAGTACTGAACAAGATTTTTCTGTTTCTTCCAACTTCGACAATACGTGGAGTGAGAGATGATCTCAAGCTTTTTGGTCCAAAGGTTATATACTTTGTATCTCTCGCTTCTTTTGAGTAAGGATTGAGATTGAAGTATTTACCTAATATTTTGTTGTTTTGCTATGGTGGGTGATTGAGTGCAATCTCAAGCTTATTGAGTAGGGTGACAAGTGTATTAGGAATAAATAAAAGACAATGTCATAGTAGTGGATTCTCAATTGAAAGGTTTTTTTCTTTTCTTTTATTTTTTCAATCTACCGTTCTTTTTACAATATAATTTAATTACTCAAAAGGG

Coding sequence (CDS)

ATGGAAGCTAAAGCTCTCCGCCAATCCACCGCCTCCGCTACCGCCGCTGAGGCCGCCTCCACATCTTCCAAATCCAATCCATTAGCTTCAGCCTCCACCACTTCCTCCTCCGTCGCAATCAGCTCTACCACCACCACTTCGAAGTCCCTAACGGACGAGCAGAATATCGACAACCTCGTCCTTCCCAGACAGGAAGTGATTCGAAGACTCCGGTTTCTGAAACAACCCATTACGCTTTTCGGGGAAGATGATGATGCTCGGCTTGACCGCTTGAAGTTCGTGCTGAAAGCGGGGCTTTTCGAGGTGGATAGTGACTTGACTGAGGGGCAAACGAATGACTTTCTTCGGGATATTGCAGAGTTGAGGAAGCGGCAGAAGACGGGGATTTTGAGCGAGCGGAAGAGGCAGAAGAAGGAGGATGGTGCTGGGGAAGAGGGAGATGGAGGTGGGGCCGATGAAGAGAGTGCCGATGGAGGGTCGAGTGGGGTTGAGACTGACAAAGATTTAAAGAGAATGAAAGCTAATTTTGATGAGTTATGTGAGGAAGATAAGATATTGGTGTTTTTTAAGAGGTTGTTAAACGAGTGGAACCAGGAATTGGATGAAATGCCTGAAGCAGAGAAGCGGACGGCCAAAGGGAAGTCTATGGTAGCAACATTCAAGCAGTGTGCTCGTTATTTACATCCTCTGTTCAAGTTTTGCAGGAAGAAGGTTCTTCCCGATGATATTCGGCAAGCATTGTTGGTAGTTGTTGAATGCTGCATGAAGCGAGACTATCTAGCTGCCATGGATCAATACATTAAGATGGCCATTGGAAATGCCCCATGGCCTATTGGTGTCACTATGGTTGGTATTCACGAACGTTCAGCTCGTGAAAAGATTTACACCAACAGTGTGGCTCATATCATGAATGATGAAACAACAAGGAAATACCTGCAATCAATCAAGAGATTGATGACGTTCTGTCAACGGCGCTATCCAACGATGCCATCCAAGGCAGTAGAATTTAACAGCTTGGCAAACGGTAGTGACCTGCAATCTCTGCTAGCAGAAGAGAGAGTTTCTGGAGGAGGCAAGCTGGGTTCTGATGAAAGGCTACGAATCATGCCTGCCCCGGAAGATAGCTAG

Protein sequence

MEAKALRQSTASATAAEAASTSSKSNPLASASTTSSSVAISSTTTTSKSLTDEQNIDNLVLPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAELRKRQKTGILSERKRQKKEDGAGEEGDGGGADEESADGGSSGVETDKDLKRMKANFDELCEEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLPDDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVAHIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGKLGSDERLRIMPAPEDS
Homology
BLAST of IVF0013677 vs. ExPASy Swiss-Prot
Match: Q99633 (Pre-mRNA-splicing factor 18 OS=Homo sapiens OX=9606 GN=PRPF18 PE=1 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 2.6e-46
Identity = 112/281 (39.86%), Postives = 166/281 (59.07%), Query Frame = 0

Query: 59  LVLPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDI 118
           + L RQEVIRRLR   +PI LFGE D     RL+      +  +  ++ +G  ND    +
Sbjct: 77  MTLSRQEVIRRLRERGEPIRLFGETDYDAFQRLR-----KIEILTPEVNKGLRNDLKAAL 136

Query: 119 AELRKRQKTGILSERKRQKKEDGAGEEGDGGGADEESADGGSSGVETDKDLKRMKANFDE 178
            ++ ++    I+          G  E G+    ++      ++ +E  + L       D+
Sbjct: 137 DKIDQQYLNEIV----------GGQEPGEEDTQNDLKVHEENTTIEELEALGESLGKGDD 196

Query: 179 LCEEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKV 238
             + D I  F K LL  W +EL+   +  KR+ +GK   AT KQ   YL PLF+  RK+ 
Sbjct: 197 HKDMDIITKFLKFLLGVWAKELNAREDYVKRSVQGKLNSATQKQTESYLRPLFRKLRKRN 256

Query: 239 LPDDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNS 298
           LP DI++++  +++  ++R+Y+ A D Y++MAIGNAPWPIGVTMVGIH R+ REKI++  
Sbjct: 257 LPADIKESITDIIKFMLQREYVKANDAYLQMAIGNAPWPIGVTMVGIHARTGREKIFSKH 316

Query: 299 VAHIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSL 340
           VAH++NDET RKY+Q +KRLMT CQ+ +PT PSK VE+N+L
Sbjct: 317 VAHVLNDETQRKYIQGLKRLMTICQKHFPTDPSKCVEYNAL 342

BLAST of IVF0013677 vs. ExPASy Swiss-Prot
Match: Q8BM39 (Pre-mRNA-splicing factor 18 OS=Mus musculus OX=10090 GN=Prpf18 PE=1 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 2.6e-46
Identity = 112/281 (39.86%), Postives = 166/281 (59.07%), Query Frame = 0

Query: 59  LVLPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDI 118
           + L RQEVIRRLR   +PI LFGE D     RL+      +  +  ++ +G  ND    +
Sbjct: 77  MTLSRQEVIRRLRERGEPIRLFGETDYDAFQRLR-----KIEILTPEVNKGLRNDLKAAL 136

Query: 119 AELRKRQKTGILSERKRQKKEDGAGEEGDGGGADEESADGGSSGVETDKDLKRMKANFDE 178
            ++ ++    I+          G  E G+    ++      ++ +E  + L       D+
Sbjct: 137 DKIDQQYLNEIV----------GGQEPGEEDTQNDLKVHEENTTIEELEALGESLGKGDD 196

Query: 179 LCEEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKV 238
             + D I  F K LL  W +EL+   +  KR+ +GK   AT KQ   YL PLF+  RK+ 
Sbjct: 197 HKDMDIITKFLKFLLGVWAKELNAREDYVKRSVQGKLNSATQKQTESYLRPLFRKLRKRN 256

Query: 239 LPDDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNS 298
           LP DI++++  +++  ++R+Y+ A D Y++MAIGNAPWPIGVTMVGIH R+ REKI++  
Sbjct: 257 LPADIKESITDIIKFMLQREYVKANDAYLQMAIGNAPWPIGVTMVGIHARTGREKIFSKH 316

Query: 299 VAHIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSL 340
           VAH++NDET RKY+Q +KRLMT CQ+ +PT PSK VE+N+L
Sbjct: 317 VAHVLNDETQRKYIQGLKRLMTICQKHFPTDPSKCVEYNAL 342

BLAST of IVF0013677 vs. ExPASy Swiss-Prot
Match: Q5RE03 (Pre-mRNA-splicing factor 18 OS=Pongo abelii OX=9601 GN=PRPF18 PE=2 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 2.6e-46
Identity = 112/281 (39.86%), Postives = 166/281 (59.07%), Query Frame = 0

Query: 59  LVLPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDI 118
           + L RQEVIRRLR   +PI LFGE D     RL+      +  +  ++ +G  ND    +
Sbjct: 77  MTLSRQEVIRRLRERGEPIRLFGETDYDAFQRLR-----KIEILTPEVNKGLRNDLKAAL 136

Query: 119 AELRKRQKTGILSERKRQKKEDGAGEEGDGGGADEESADGGSSGVETDKDLKRMKANFDE 178
            ++ ++    I+          G  E G+    ++      ++ +E  + L       D+
Sbjct: 137 DKIDQQYLNEIV----------GGQEPGEEDTQNDLKVHEENTTIEELEALGESLGKGDD 196

Query: 179 LCEEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKV 238
             + D I  F K LL  W +EL+   +  KR+ +GK   AT KQ   YL PLF+  RK+ 
Sbjct: 197 HKDMDIITKFLKFLLGVWAKELNAREDYVKRSVQGKLNSATQKQTESYLRPLFRKLRKRN 256

Query: 239 LPDDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNS 298
           LP DI++++  +++  ++R+Y+ A D Y++MAIGNAPWPIGVTMVGIH R+ REKI++  
Sbjct: 257 LPADIKESITDIIKFMLQREYVKANDAYLQMAIGNAPWPIGVTMVGIHARTGREKIFSKH 316

Query: 299 VAHIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSL 340
           VAH++NDET RKY+Q +KRLMT CQ+ +PT PSK VE+N+L
Sbjct: 317 VAHVLNDETQRKYIQGLKRLMTICQKHFPTDPSKCVEYNAL 342

BLAST of IVF0013677 vs. ExPASy Swiss-Prot
Match: Q2HJ41 (Pre-mRNA-splicing factor 18 OS=Bos taurus OX=9913 GN=PRPF18 PE=2 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 4.5e-46
Identity = 111/281 (39.50%), Postives = 166/281 (59.07%), Query Frame = 0

Query: 59  LVLPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDI 118
           + L RQEVIRRLR   +PI LFGE D     RL+      +  +  ++ +G  ND    +
Sbjct: 77  MTLSRQEVIRRLRERGEPIRLFGETDYDAFQRLR-----KIEILTPEVNKGLRNDLKAAL 136

Query: 119 AELRKRQKTGILSERKRQKKEDGAGEEGDGGGADEESADGGSSGVETDKDLKRMKANFDE 178
            ++ ++    ++          G  E G+    ++      ++ +E  + L       D+
Sbjct: 137 DKIDQQYLNELV----------GGQEPGEEDTQNDLKVHEENTTIEELEALGESLGKGDD 196

Query: 179 LCEEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKV 238
             + D I  F K LL  W +EL+   +  KR+ +GK   AT KQ   YL PLF+  RK+ 
Sbjct: 197 HKDMDIITKFLKFLLGVWAKELNAREDYVKRSVQGKLNSATQKQTESYLRPLFRKLRKRN 256

Query: 239 LPDDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNS 298
           LP DI++++  +++  ++R+Y+ A D Y++MAIGNAPWPIGVTMVGIH R+ REKI++  
Sbjct: 257 LPADIKESITDIIKFMLQREYVKANDAYLQMAIGNAPWPIGVTMVGIHARTGREKIFSKH 316

Query: 299 VAHIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSL 340
           VAH++NDET RKY+Q +KRLMT CQ+ +PT PSK VE+N+L
Sbjct: 317 VAHVLNDETQRKYIQGLKRLMTICQKHFPTDPSKCVEYNAL 342

BLAST of IVF0013677 vs. ExPASy Swiss-Prot
Match: Q9JKB8 (Pre-mRNA-splicing factor 18 OS=Rattus norvegicus OX=10116 GN=Prpf18 PE=2 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 4.5e-46
Identity = 111/281 (39.50%), Postives = 166/281 (59.07%), Query Frame = 0

Query: 59  LVLPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDI 118
           + L RQEVIRRLR   +P+ LFGE D     RL+      +  +  ++ +G  ND    +
Sbjct: 77  MTLSRQEVIRRLRERGEPMRLFGETDYDAFQRLR-----KIEILTPEVNKGLRNDLKAAL 136

Query: 119 AELRKRQKTGILSERKRQKKEDGAGEEGDGGGADEESADGGSSGVETDKDLKRMKANFDE 178
            ++ ++    I+          G  E G+    ++      ++ +E  + L       D+
Sbjct: 137 DKIDQQYLNEIV----------GGQEPGEEDTQNDLKVHEENTTIEELEALGESLGKGDD 196

Query: 179 LCEEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKV 238
             + D I  F K LL  W +EL+   +  KR+ +GK   AT KQ   YL PLF+  RK+ 
Sbjct: 197 HKDMDIITKFLKFLLGVWAKELNSREDYVKRSVQGKLNSATQKQTESYLRPLFRKLRKRN 256

Query: 239 LPDDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNS 298
           LP DI++++  +++  ++R+Y+ A D Y++MAIGNAPWPIGVTMVGIH R+ REKI++  
Sbjct: 257 LPADIKESITDIIKFMLQREYVKANDAYLQMAIGNAPWPIGVTMVGIHARTGREKIFSKH 316

Query: 299 VAHIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSL 340
           VAH++NDET RKY+Q +KRLMT CQ+ +PT PSK VE+N+L
Sbjct: 317 VAHVLNDETQRKYIQGLKRLMTICQKHFPTDPSKCVEYNAL 342

BLAST of IVF0013677 vs. ExPASy TrEMBL
Match: A0A5D3DRE9 (Pre-mRNA-splicing factor 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold111G001610 PE=3 SV=1)

HSP 1 Score: 700.7 bits (1807), Expect = 3.4e-198
Identity = 374/375 (99.73%), Postives = 375/375 (100.00%), Query Frame = 0

Query: 1   MEAKALRQSTASATAAEAASTSSKSNPLASASTTSSSVAISSTTTTSKSLTDEQNIDNLV 60
           +EAKALRQSTASATAAEAASTSSKSNPLASASTTSSSVAISSTTTTSKSLTDEQNIDNLV
Sbjct: 46  LEAKALRQSTASATAAEAASTSSKSNPLASASTTSSSVAISSTTTTSKSLTDEQNIDNLV 105

Query: 61  LPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAE 120
           LPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAE
Sbjct: 106 LPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAE 165

Query: 121 LRKRQKTGILSERKRQKKEDGAGEEGDGGGADEESADGGSSGVETDKDLKRMKANFDELC 180
           LRKRQKTGILSERKRQKKEDGAGEEGDGGGADEESADGGSSGVETDKDLKRMKANFDELC
Sbjct: 166 LRKRQKTGILSERKRQKKEDGAGEEGDGGGADEESADGGSSGVETDKDLKRMKANFDELC 225

Query: 181 EEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLP 240
           EEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLP
Sbjct: 226 EEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLP 285

Query: 241 DDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVA 300
           DDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVA
Sbjct: 286 DDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVA 345

Query: 301 HIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGKL 360
           HIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGKL
Sbjct: 346 HIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGKL 405

Query: 361 GSDERLRIMPAPEDS 376
           GSDERLRIMPAPEDS
Sbjct: 406 GSDERLRIMPAPEDS 420

BLAST of IVF0013677 vs. ExPASy TrEMBL
Match: A0A1S3BS26 (Pre-mRNA-splicing factor 18 OS=Cucumis melo OX=3656 GN=LOC103492665 PE=3 SV=1)

HSP 1 Score: 698.7 bits (1802), Expect = 1.3e-197
Identity = 373/375 (99.47%), Postives = 374/375 (99.73%), Query Frame = 0

Query: 1   MEAKALRQSTASATAAEAASTSSKSNPLASASTTSSSVAISSTTTTSKSLTDEQNIDNLV 60
           +EAKALRQSTASATAAEAASTSSKSNPLASASTTSSSVAISSTTTTSKSLTDEQNIDNLV
Sbjct: 46  LEAKALRQSTASATAAEAASTSSKSNPLASASTTSSSVAISSTTTTSKSLTDEQNIDNLV 105

Query: 61  LPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAE 120
           LPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAE
Sbjct: 106 LPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAE 165

Query: 121 LRKRQKTGILSERKRQKKEDGAGEEGDGGGADEESADGGSSGVETDKDLKRMKANFDELC 180
           LRKRQKTGILSERKRQKKEDGAGEEGDGGG DEESADGGSSGVETDKDLKRMKANFDELC
Sbjct: 166 LRKRQKTGILSERKRQKKEDGAGEEGDGGGPDEESADGGSSGVETDKDLKRMKANFDELC 225

Query: 181 EEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLP 240
           EEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLP
Sbjct: 226 EEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLP 285

Query: 241 DDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVA 300
           DDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVA
Sbjct: 286 DDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVA 345

Query: 301 HIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGKL 360
           HIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGKL
Sbjct: 346 HIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGKL 405

Query: 361 GSDERLRIMPAPEDS 376
           GSDERLRIMPAPEDS
Sbjct: 406 GSDERLRIMPAPEDS 420

BLAST of IVF0013677 vs. ExPASy TrEMBL
Match: A0A0A0K7C9 (Pre-mRNA-splicing factor 18 OS=Cucumis sativus OX=3659 GN=Csa_7G388420 PE=3 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 1.2e-195
Identity = 369/375 (98.40%), Postives = 371/375 (98.93%), Query Frame = 0

Query: 1   MEAKALRQSTASATAAEAASTSSKSNPLASASTTSSSVAISSTTTTSKSLTDEQNIDNLV 60
           +EAKALRQST SATAAEA STSSKSNPLASASTTSSSVA +STTTTSKSLTDEQNIDNLV
Sbjct: 46  LEAKALRQSTTSATAAEAVSTSSKSNPLASASTTSSSVATNSTTTTSKSLTDEQNIDNLV 105

Query: 61  LPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAE 120
           LPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAE
Sbjct: 106 LPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAE 165

Query: 121 LRKRQKTGILSERKRQKKEDGAGEEGDGGGADEESADGGSSGVETDKDLKRMKANFDELC 180
           LRKRQKTGILSERKRQKKEDGAGEEGDGGGADEESADGGSSGVETDKDLKRMKANFDELC
Sbjct: 166 LRKRQKTGILSERKRQKKEDGAGEEGDGGGADEESADGGSSGVETDKDLKRMKANFDELC 225

Query: 181 EEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLP 240
           EEDKILVFFKRLLNEW QELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLP
Sbjct: 226 EEDKILVFFKRLLNEWKQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLP 285

Query: 241 DDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVA 300
           DDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVA
Sbjct: 286 DDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVA 345

Query: 301 HIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGKL 360
           HIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGKL
Sbjct: 346 HIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGKL 405

Query: 361 GSDERLRIMPAPEDS 376
           GSDERLRIMPAPEDS
Sbjct: 406 GSDERLRIMPAPEDS 420

BLAST of IVF0013677 vs. ExPASy TrEMBL
Match: A0A6J1GQ96 (Pre-mRNA-splicing factor 18 OS=Cucurbita moschata OX=3662 GN=LOC111456490 PE=3 SV=1)

HSP 1 Score: 669.5 bits (1726), Expect = 8.4e-189
Identity = 357/375 (95.20%), Postives = 364/375 (97.07%), Query Frame = 0

Query: 1   MEAKALRQSTASATAAEAASTSSKSNPLASASTTSSSVAISSTTTTSKSLTDEQNIDNLV 60
           +EAKALRQS+ S+  AEA S SSKSN LASASTTSSS AI+ST TTSKSLTDEQNIDNLV
Sbjct: 46  LEAKALRQSSTSSPNAEATSASSKSNSLASASTTSSSAAINSTATTSKSLTDEQNIDNLV 105

Query: 61  LPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAE 120
           L RQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAE
Sbjct: 106 LTRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAE 165

Query: 121 LRKRQKTGILSERKRQKKEDGAGEEGDGGGADEESADGGSSGVETDKDLKRMKANFDELC 180
           LRKRQKTGILSERKRQKKEDGAGEEGDGGGADE+SADGGSSGV+ DKDLKR+KANFDELC
Sbjct: 166 LRKRQKTGILSERKRQKKEDGAGEEGDGGGADEDSADGGSSGVDADKDLKRIKANFDELC 225

Query: 181 EEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLP 240
           EEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLP
Sbjct: 226 EEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLP 285

Query: 241 DDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVA 300
           DDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVA
Sbjct: 286 DDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVA 345

Query: 301 HIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGKL 360
           HIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGK 
Sbjct: 346 HIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGKQ 405

Query: 361 GSDERLRIMPAPEDS 376
           GSDERLRIMPAPEDS
Sbjct: 406 GSDERLRIMPAPEDS 420

BLAST of IVF0013677 vs. ExPASy TrEMBL
Match: A0A6J1JZG6 (Pre-mRNA-splicing factor 18 OS=Cucurbita maxima OX=3661 GN=LOC111488824 PE=3 SV=1)

HSP 1 Score: 667.9 bits (1722), Expect = 2.4e-188
Identity = 355/375 (94.67%), Postives = 363/375 (96.80%), Query Frame = 0

Query: 1   MEAKALRQSTASATAAEAASTSSKSNPLASASTTSSSVAISSTTTTSKSLTDEQNIDNLV 60
           +EAKALRQS+ S+  AEA S SSKSN LASAS TSSS AI+ST TTSKS+TDEQNIDNLV
Sbjct: 46  LEAKALRQSSTSSPNAEATSASSKSNSLASASATSSSAAINSTATTSKSVTDEQNIDNLV 105

Query: 61  LPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAE 120
           LPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAE
Sbjct: 106 LPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAE 165

Query: 121 LRKRQKTGILSERKRQKKEDGAGEEGDGGGADEESADGGSSGVETDKDLKRMKANFDELC 180
           LRKRQKTGILSERKRQKKEDGAGEEGDGGGADE+SADGGSSG + DKDLKR+KANFDELC
Sbjct: 166 LRKRQKTGILSERKRQKKEDGAGEEGDGGGADEDSADGGSSGADADKDLKRIKANFDELC 225

Query: 181 EEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLP 240
           EEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLP
Sbjct: 226 EEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLP 285

Query: 241 DDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVA 300
           DDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVA
Sbjct: 286 DDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVA 345

Query: 301 HIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGKL 360
           HIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGK 
Sbjct: 346 HIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGKQ 405

Query: 361 GSDERLRIMPAPEDS 376
           GSDERLRIMPAPEDS
Sbjct: 406 GSDERLRIMPAPEDS 420

BLAST of IVF0013677 vs. NCBI nr
Match: KAA0064068.1 (pre-mRNA-splicing factor 18 [Cucumis melo var. makuwa] >TYK26227.1 pre-mRNA-splicing factor 18 [Cucumis melo var. makuwa])

HSP 1 Score: 699 bits (1803), Expect = 3.78e-252
Identity = 374/375 (99.73%), Postives = 375/375 (100.00%), Query Frame = 0

Query: 1   MEAKALRQSTASATAAEAASTSSKSNPLASASTTSSSVAISSTTTTSKSLTDEQNIDNLV 60
           +EAKALRQSTASATAAEAASTSSKSNPLASASTTSSSVAISSTTTTSKSLTDEQNIDNLV
Sbjct: 46  LEAKALRQSTASATAAEAASTSSKSNPLASASTTSSSVAISSTTTTSKSLTDEQNIDNLV 105

Query: 61  LPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAE 120
           LPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAE
Sbjct: 106 LPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAE 165

Query: 121 LRKRQKTGILSERKRQKKEDGAGEEGDGGGADEESADGGSSGVETDKDLKRMKANFDELC 180
           LRKRQKTGILSERKRQKKEDGAGEEGDGGGADEESADGGSSGVETDKDLKRMKANFDELC
Sbjct: 166 LRKRQKTGILSERKRQKKEDGAGEEGDGGGADEESADGGSSGVETDKDLKRMKANFDELC 225

Query: 181 EEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLP 240
           EEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLP
Sbjct: 226 EEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLP 285

Query: 241 DDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVA 300
           DDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVA
Sbjct: 286 DDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVA 345

Query: 301 HIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGKL 360
           HIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGKL
Sbjct: 346 HIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGKL 405

Query: 361 GSDERLRIMPAPEDS 375
           GSDERLRIMPAPEDS
Sbjct: 406 GSDERLRIMPAPEDS 420

BLAST of IVF0013677 vs. NCBI nr
Match: XP_008451349.1 (PREDICTED: pre-mRNA-splicing factor 18 [Cucumis melo])

HSP 1 Score: 697 bits (1798), Expect = 2.18e-251
Identity = 373/375 (99.47%), Postives = 374/375 (99.73%), Query Frame = 0

Query: 1   MEAKALRQSTASATAAEAASTSSKSNPLASASTTSSSVAISSTTTTSKSLTDEQNIDNLV 60
           +EAKALRQSTASATAAEAASTSSKSNPLASASTTSSSVAISSTTTTSKSLTDEQNIDNLV
Sbjct: 46  LEAKALRQSTASATAAEAASTSSKSNPLASASTTSSSVAISSTTTTSKSLTDEQNIDNLV 105

Query: 61  LPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAE 120
           LPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAE
Sbjct: 106 LPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAE 165

Query: 121 LRKRQKTGILSERKRQKKEDGAGEEGDGGGADEESADGGSSGVETDKDLKRMKANFDELC 180
           LRKRQKTGILSERKRQKKEDGAGEEGDGGG DEESADGGSSGVETDKDLKRMKANFDELC
Sbjct: 166 LRKRQKTGILSERKRQKKEDGAGEEGDGGGPDEESADGGSSGVETDKDLKRMKANFDELC 225

Query: 181 EEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLP 240
           EEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLP
Sbjct: 226 EEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLP 285

Query: 241 DDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVA 300
           DDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVA
Sbjct: 286 DDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVA 345

Query: 301 HIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGKL 360
           HIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGKL
Sbjct: 346 HIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGKL 405

Query: 361 GSDERLRIMPAPEDS 375
           GSDERLRIMPAPEDS
Sbjct: 406 GSDERLRIMPAPEDS 420

BLAST of IVF0013677 vs. NCBI nr
Match: XP_004146980.1 (pre-mRNA-splicing factor 18 [Cucumis sativus] >KGN44809.1 hypothetical protein Csa_015983 [Cucumis sativus])

HSP 1 Score: 690 bits (1781), Expect = 8.48e-249
Identity = 369/375 (98.40%), Postives = 371/375 (98.93%), Query Frame = 0

Query: 1   MEAKALRQSTASATAAEAASTSSKSNPLASASTTSSSVAISSTTTTSKSLTDEQNIDNLV 60
           +EAKALRQST SATAAEA STSSKSNPLASASTTSSSVA +STTTTSKSLTDEQNIDNLV
Sbjct: 46  LEAKALRQSTTSATAAEAVSTSSKSNPLASASTTSSSVATNSTTTTSKSLTDEQNIDNLV 105

Query: 61  LPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAE 120
           LPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAE
Sbjct: 106 LPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAE 165

Query: 121 LRKRQKTGILSERKRQKKEDGAGEEGDGGGADEESADGGSSGVETDKDLKRMKANFDELC 180
           LRKRQKTGILSERKRQKKEDGAGEEGDGGGADEESADGGSSGVETDKDLKRMKANFDELC
Sbjct: 166 LRKRQKTGILSERKRQKKEDGAGEEGDGGGADEESADGGSSGVETDKDLKRMKANFDELC 225

Query: 181 EEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLP 240
           EEDKILVFFKRLLNEW QELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLP
Sbjct: 226 EEDKILVFFKRLLNEWKQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLP 285

Query: 241 DDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVA 300
           DDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVA
Sbjct: 286 DDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVA 345

Query: 301 HIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGKL 360
           HIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGKL
Sbjct: 346 HIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGKL 405

Query: 361 GSDERLRIMPAPEDS 375
           GSDERLRIMPAPEDS
Sbjct: 406 GSDERLRIMPAPEDS 420

BLAST of IVF0013677 vs. NCBI nr
Match: XP_038897671.1 (pre-mRNA-splicing factor 18 [Benincasa hispida] >XP_038897672.1 pre-mRNA-splicing factor 18 [Benincasa hispida])

HSP 1 Score: 678 bits (1749), Expect = 6.35e-244
Identity = 362/375 (96.53%), Postives = 367/375 (97.87%), Query Frame = 0

Query: 1   MEAKALRQSTASATAAEAASTSSKSNPLASASTTSSSVAISSTTTTSKSLTDEQNIDNLV 60
           +EAKALRQST SA AAEA STSSKSNPLASASTTSSSVAI+ST TTSKSLTDEQNIDNLV
Sbjct: 46  LEAKALRQSTTSAAAAEATSTSSKSNPLASASTTSSSVAINSTATTSKSLTDEQNIDNLV 105

Query: 61  LPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAE 120
           LPRQEVIRRLRFLKQPIT+FGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAE
Sbjct: 106 LPRQEVIRRLRFLKQPITIFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAE 165

Query: 121 LRKRQKTGILSERKRQKKEDGAGEEGDGGGADEESADGGSSGVETDKDLKRMKANFDELC 180
           LRKRQKTGILSERKRQKKEDGAGEEGDGGGADEESADGGSSGV+ DKDL RMKANFDELC
Sbjct: 166 LRKRQKTGILSERKRQKKEDGAGEEGDGGGADEESADGGSSGVDADKDLNRMKANFDELC 225

Query: 181 EEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLP 240
           EEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLP
Sbjct: 226 EEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLP 285

Query: 241 DDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVA 300
           DDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVA
Sbjct: 286 DDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVA 345

Query: 301 HIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGKL 360
           HIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGK 
Sbjct: 346 HIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGKP 405

Query: 361 GSDERLRIMPAPEDS 375
           GSDERLRIMPA +DS
Sbjct: 406 GSDERLRIMPASDDS 420

BLAST of IVF0013677 vs. NCBI nr
Match: XP_023549183.1 (pre-mRNA-splicing factor 18-like [Cucurbita pepo subsp. pepo] >XP_023549184.1 pre-mRNA-splicing factor 18-like [Cucurbita pepo subsp. pepo] >XP_023549185.1 pre-mRNA-splicing factor 18-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 669 bits (1727), Expect = 1.42e-240
Identity = 357/375 (95.20%), Postives = 365/375 (97.33%), Query Frame = 0

Query: 1   MEAKALRQSTASATAAEAASTSSKSNPLASASTTSSSVAISSTTTTSKSLTDEQNIDNLV 60
           +EAKALRQS+ ++  AEA S SSKSN LASASTTSSS AI+ST TTSKSLTDEQNIDNLV
Sbjct: 46  LEAKALRQSSTTSPNAEATSASSKSNSLASASTTSSSAAINSTATTSKSLTDEQNIDNLV 105

Query: 61  LPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAE 120
           LPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAE
Sbjct: 106 LPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIAE 165

Query: 121 LRKRQKTGILSERKRQKKEDGAGEEGDGGGADEESADGGSSGVETDKDLKRMKANFDELC 180
           LRKRQKTGILSERKRQKKEDGAGEEGDGGGADE+SADGGSSGV+ DKDLKR+KANFDELC
Sbjct: 166 LRKRQKTGILSERKRQKKEDGAGEEGDGGGADEDSADGGSSGVDADKDLKRIKANFDELC 225

Query: 181 EEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLP 240
           EEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLP
Sbjct: 226 EEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLP 285

Query: 241 DDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVA 300
           DDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVA
Sbjct: 286 DDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNSVA 345

Query: 301 HIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGKL 360
           HIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGK 
Sbjct: 346 HIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGGKQ 405

Query: 361 GSDERLRIMPAPEDS 375
           GSDERLRIMPAPEDS
Sbjct: 406 GSDERLRIMPAPEDS 420

BLAST of IVF0013677 vs. TAIR 10
Match: AT1G03140.1 (splicing factor Prp18 family protein )

HSP 1 Score: 481.9 bits (1239), Expect = 4.8e-136
Identity = 263/378 (69.58%), Postives = 314/378 (83.07%), Query Frame = 0

Query: 2   EAKALRQSTASATAAEAASTSSK--SNPLASASTTSSSVAISSTTTTSKSLTDEQNIDNL 61
           E KA R++ A+A+  +  S+ S   S+  A+++++ SS + ++    SK+LTDE    NL
Sbjct: 47  ELKAQRRAAAAASGGDGKSSGSAPGSSNAATSASSKSSASDAAAIADSKALTDE----NL 106

Query: 62  VLPRQEVIRRLRFLKQPITLFGEDDDARLDRLKFVLKAGLFEVDSDLTEGQTNDFLRDIA 121
           +LPRQEVIRRLRFLKQP+TLFGEDD +RLDRLK+VLK GLFEVDSD+TEGQTNDFLRDIA
Sbjct: 107 ILPRQEVIRRLRFLKQPMTLFGEDDQSRLDRLKYVLKEGLFEVDSDMTEGQTNDFLRDIA 166

Query: 122 ELRKRQKTGILSERKRQKKEDGAGEEGDGGGADEESADGG-SSGVETDKDLKRMKANFDE 181
           EL+KRQK+G++ +RKR+ +++   +EGD G   E+   GG SS V+ DKD+KR+KANF++
Sbjct: 167 ELKKRQKSGMMGDRKRKSRDERGRDEGDRGETREDELSGGESSDVDADKDMKRLKANFED 226

Query: 182 LCEEDKILVFFKRLLNEWNQELDEMPEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKV 241
           LC+EDKILVF+K+LL EW QELD M   E+RTAKGK MVATFKQCARYL PLF  CRKK 
Sbjct: 227 LCDEDKILVFYKKLLIEWKQELDAMENTERRTAKGKQMVATFKQCARYLVPLFNLCRKKG 286

Query: 242 LPDDIRQALLVVVECCMKRDYLAAMDQYIKMAIGNAPWPIGVTMVGIHERSAREKIYTNS 301
           LP DIRQAL+V+V  C+KRDYLAAMD YIK+AIGNAPWPIGVTMVGIHERSAREKIYTNS
Sbjct: 287 LPADIRQALMVMVNHCIKRDYLAAMDHYIKLAIGNAPWPIGVTMVGIHERSAREKIYTNS 346

Query: 302 VAHIMNDETTRKYLQSIKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERVSGGG 361
           VAHIMNDETTRKYLQS+KRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEER  GG 
Sbjct: 347 VAHIMNDETTRKYLQSVKRLMTFCQRRYPTMPSKAVEFNSLANGSDLQSLLAEERFFGGN 406

Query: 362 K-LGSDERLRIMPAPEDS 376
           +   S+ERLR+MP+  +S
Sbjct: 407 REQVSEERLRLMPSQSES 420

BLAST of IVF0013677 vs. TAIR 10
Match: AT1G54590.1 (INVOLVED IN: RNA splicing; LOCATED IN: spliceosomal complex; CONTAINS InterPro DOMAIN/s: Prp18 (InterPro:IPR004098); BEST Arabidopsis thaliana protein match is: splicing factor Prp18 family protein (TAIR:AT1G03140.1); Has 388 Blast hits to 388 proteins in 196 species: Archae - 0; Bacteria - 0; Metazoa - 136; Fungi - 132; Plants - 48; Viruses - 0; Other Eukaryotes - 72 (source: NCBI BLink). )

HSP 1 Score: 327.8 bits (839), Expect = 1.1e-89
Identity = 182/290 (62.76%), Postives = 225/290 (77.59%), Query Frame = 0

Query: 87  RLDRLKFVLKAGLF-EVDSDLTEGQTNDFLRDIAELRKRQK-TGILSERKRQKKEDGAGE 146
           RLDRLK+VLK GLF EVD+D+T+G+TNDFLRDI EL+KR++ +G++++RKR+   D    
Sbjct: 86  RLDRLKYVLKEGLFEEVDNDMTQGETNDFLRDITELKKRERSSGLMNDRKRKTSND---- 145

Query: 147 EGDGGGADEESADGGSSGVETDKDLKRM-KANFDELCEEDKILVFFKRLLNEWNQELDEM 206
             +  GA++E             DLK + +ANF++LC+EDKILVF K+LL EW QEL+ M
Sbjct: 146 -DELIGAEKE-------------DLKLLEEANFEDLCDEDKILVFCKKLLLEWKQELEAM 205

Query: 207 PEAEKRTAKGKSMVATFKQCARYLHPLFKFCRKKVLPDDIRQALLVVVECCMKRDYLAAM 266
              E+RTA GK M+ATF QCARYL PLF  CR K LP DIRQ L+V+V C +KRDYL A 
Sbjct: 206 ENTERRTAIGKQMLATFNQCARYLTPLFHLCRNKCLPADIRQGLMVMVNCWIKRDYLDAT 265

Query: 267 DQYIKMAIGNAPWPIGVTMVGIHERSAREKIYT-NSVAHIMNDETTRKYLQSIKRLMTFC 326
            Q+IK+AIGNAPWPIGVTMVGIHERSAREKI T +SVAHIMN+ETTRKYLQS+KRLMTFC
Sbjct: 266 AQFIKLAIGNAPWPIGVTMVGIHERSAREKISTSSSVAHIMNNETTRKYLQSVKRLMTFC 325

Query: 327 QRRYPTMPSKAVEFNSLANGSDLQSLLAEER-VSGGGKLGSDERLRIMPA 372
           QRRY  +PSK++EFNSLANGS+L SLLAEER  +   +  S+ERL +MP+
Sbjct: 326 QRRYSALPSKSIEFNSLANGSNLHSLLAEERFFAADRERVSEERLWLMPS 357

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q996332.6e-4639.86Pre-mRNA-splicing factor 18 OS=Homo sapiens OX=9606 GN=PRPF18 PE=1 SV=1[more]
Q8BM392.6e-4639.86Pre-mRNA-splicing factor 18 OS=Mus musculus OX=10090 GN=Prpf18 PE=1 SV=1[more]
Q5RE032.6e-4639.86Pre-mRNA-splicing factor 18 OS=Pongo abelii OX=9601 GN=PRPF18 PE=2 SV=1[more]
Q2HJ414.5e-4639.50Pre-mRNA-splicing factor 18 OS=Bos taurus OX=9913 GN=PRPF18 PE=2 SV=1[more]
Q9JKB84.5e-4639.50Pre-mRNA-splicing factor 18 OS=Rattus norvegicus OX=10116 GN=Prpf18 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3DRE93.4e-19899.73Pre-mRNA-splicing factor 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3BS261.3e-19799.47Pre-mRNA-splicing factor 18 OS=Cucumis melo OX=3656 GN=LOC103492665 PE=3 SV=1[more]
A0A0A0K7C91.2e-19598.40Pre-mRNA-splicing factor 18 OS=Cucumis sativus OX=3659 GN=Csa_7G388420 PE=3 SV=1[more]
A0A6J1GQ968.4e-18995.20Pre-mRNA-splicing factor 18 OS=Cucurbita moschata OX=3662 GN=LOC111456490 PE=3 S... [more]
A0A6J1JZG62.4e-18894.67Pre-mRNA-splicing factor 18 OS=Cucurbita maxima OX=3661 GN=LOC111488824 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
KAA0064068.13.78e-25299.73pre-mRNA-splicing factor 18 [Cucumis melo var. makuwa] >TYK26227.1 pre-mRNA-spli... [more]
XP_008451349.12.18e-25199.47PREDICTED: pre-mRNA-splicing factor 18 [Cucumis melo][more]
XP_004146980.18.48e-24998.40pre-mRNA-splicing factor 18 [Cucumis sativus] >KGN44809.1 hypothetical protein C... [more]
XP_038897671.16.35e-24496.53pre-mRNA-splicing factor 18 [Benincasa hispida] >XP_038897672.1 pre-mRNA-splicin... [more]
XP_023549183.11.42e-24095.20pre-mRNA-splicing factor 18-like [Cucurbita pepo subsp. pepo] >XP_023549184.1 pr... [more]
Match NameE-valueIdentityDescription
AT1G03140.14.8e-13669.58splicing factor Prp18 family protein [more]
AT1G54590.11.1e-8962.76INVOLVED IN: RNA splicing; LOCATED IN: spliceosomal complex; CONTAINS InterPro D... [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014906Pre-mRNA processing factor 4 (PRP4)-likeSMARTSM00500pr04_2coord: 61..112
e-value: 1.8E-21
score: 87.3
IPR014906Pre-mRNA processing factor 4 (PRP4)-likePFAMPF08799PRP4coord: 66..92
e-value: 1.1E-11
score: 44.1
IPR004098Prp18PFAMPF02840Prp18coord: 187..328
e-value: 1.4E-62
score: 210.1
IPR036285PRP4-like superfamilyGENE3D4.10.280.110coord: 30..109
e-value: 6.7E-16
score: 59.5
IPR036285PRP4-like superfamilySUPERFAMILY158230PRP4-likecoord: 55..96
NoneNo IPR availableGENE3D1.20.940.10Functional domain of the splicing factor Prp18coord: 157..330
e-value: 2.8E-59
score: 201.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 129..165
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 354..375
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 129..145
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 7..49
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..49
NoneNo IPR availablePANTHERPTHR13007:SF22BNAA09G50970D PROTEINcoord: 3..371
NoneNo IPR availableSUPERFAMILY47938Functional domain of the splicing factor Prp18coord: 162..328
IPR039979Pre-mRNA-splicing factor 18PANTHERPTHR13007PRE-MRNA SPLICING FACTOR-RELATEDcoord: 3..371

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0013677.1IVF0013677.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000350 generation of catalytic spliceosome for second transesterification step
biological_process GO:0008380 RNA splicing
cellular_component GO:0071021 U2-type post-spliceosomal complex
cellular_component GO:0046540 U4/U6 x U5 tri-snRNP complex
cellular_component GO:0005682 U5 snRNP
cellular_component GO:0005681 spliceosomal complex