Cucsat.G4436 (gene) Cucumber (B10) v3

BLAST of Cucsat.G4436 vs. ExPASy Swiss-Prot
Match: Q9LM76 (U-box domain-containing protein 44 OS=Arabidopsis thaliana OX=3702 GN=PUB44 PE=1 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 3.1e-39
Identity = 190/734 (25.89%), Postives = 345/734 (47.00%), Query Frame = 0

Query: 112 TVEVSTQFKTK-IGELHKELMNMKFDENCSPTSTSSRTTEFICDLRVEEIEEE---RTSV 171
           T+E    F+ + I +  KE  +     +C  TS    +T+    + +    EE   R   
Sbjct: 41  TLENGRTFEREAIEKWFKECRDSGRPPSCPLTSQELTSTDVSASIALRNTIEEWRSRNDA 100

Query: 172 KACDIALH-LKYGN-DDEFKLAVAGLKDLIQNKNVDDGWLNEEGIVSILLNRLGSNKSVN 231
              DIA   L  GN + +   A+  ++ + +    +   +    ++ ++++ L S     
Sbjct: 101 AKLDIARQSLFLGNAETDILQALMHVRQICRTIRSNRHGVRNSQLIHMIIDMLKSTSHRV 160

Query: 232 RPLIIQVLRYLVWNNPASKEMMADVGPLSTLVKSLAGDEEERRE-VVGLLLELCDFVNVR 291
           R   +Q L+ +V  +  SK ++A+   + TLVK L+ +  + RE  V LL EL     + 
Sbjct: 161 RYKALQTLQVVVEGDDESKAIVAEGDTVRTLVKFLSHEPSKGREAAVSLLFELSKSEALC 220

Query: 292 RRLGRIQGCIVMLVSILKGDDQIASY--DARKLLNVLSGNTQNVLYMAEAGYFKPMVQHL 351
            ++G I G +++LV +   + +  S    A + L  +  + + V  MA  G  +P++  L
Sbjct: 221 EKIGSIHGALILLVGLTSSNSENVSIVEKADRTLENMERSEEIVRQMASYGRLQPLLGKL 280

Query: 352 IEGSDMNKILMATGLSRMEHTEQSKASLGEEGVIEPLVQMFRTEKLEAKLSALSALQSLS 411
           +EGS   K+ MA+ L  +      K  L  + V   LV + R+  +  + +AL AL  +S
Sbjct: 281 LEGSPETKLSMASFLGELPLNNDVKV-LVAQTVGSSLVDLMRSGDMPQREAALKALNKIS 340

Query: 412 GLKENVQRLISSGIVVSLLQLLFSV--TSVLMTLREPAAAILAKISE-----SESILTNY 471
             + + + LIS GI+  L++ LF V   ++ + L+E +A ILA I        ++ L + 
Sbjct: 341 SFEGSAKVLISKGILPPLIKDLFYVGPNNLPIRLKEVSATILANIVNIGYDFDKATLVSE 400

Query: 472 DVALQMLSLLNLSSPIIQNHLLQALNNIAANPSAL-EVRKKMVESGAIQLLFPFL-MEDN 531
           +    +L L++ + P IQ  LL+ L  + + P  + +V   +  SGAI  L  F+ + +N
Sbjct: 401 NRVENLLHLISNTGPAIQCKLLEVLVGLTSCPKTVPKVVYAIKTSGAIISLVQFIEVREN 460

Query: 532 TKVKSGALKLLYTLSKDAPEELEESHI-------SLILNIISSTDCKSERVFAVGILSNV 591
             ++  ++KLL+ LS    EEL ++         SL+  I   T    E+  A G+L+ +
Sbjct: 461 DDLRLASIKLLHNLSPFMSEELAKALCGTAGQLGSLVAIISEKTPITEEQAAAAGLLAEL 520

Query: 592 PVTQKKITDLLRKANLVPILISI---MNSSSANSDIFASLSSESVAGLLVRFTNPFDRKL 651
           P     +T  + +      +IS    +         F +   E +  +L R T  F+++ 
Sbjct: 521 PDRDLGLTQEMLEVGAFEKIISKVFGIRQGDIKGMRFVNPFLEGLVRILARITFVFNKEA 580

Query: 652 QLHSV--EQGVIPLLVKLLSSE-SPVAQSKAAISLAQLSQNSLSLSKSRT--------SR 711
           +  +   E  V  L + LL S      Q  +A++L  LS  S+ L++           S 
Sbjct: 581 RAINFCREHDVASLFLHLLQSNGQDNIQMVSAMALENLSLESIKLTRMPDPPPVNYCGSI 640

Query: 712 WLCV--PPSKDSICEVHGRQCLTKRTFCLVKADAIRPMIEILEGKESEVDEAVLSALTTL 771
           + CV  P   + +C++H   C  + TFCLV+  A+  ++ +L+ +  +V EA L+AL++L
Sbjct: 641 FSCVRKPHVVNGLCKIHQGICSLRETFCLVEGGAVEKLVALLDHENVKVVEAALAALSSL 700

Query: 772 LEDEI-CDNGSNYVVKMSGVEAILKVLGSGHID-AQQKALWILERIFRIEE--HRVKYGE 801
           LED +  + G   + +  G+  IL VL     +   ++A+W++ERI RIE+    V   +
Sbjct: 701 LEDGLDVEKGVKILDEADGIRHILNVLRENRTERLTRRAVWMVERILRIEDIAREVAEEQ 760

BLAST of Cucsat.G4436 vs. ExPASy Swiss-Prot
Match: Q9SFX2 (U-box domain-containing protein 43 OS=Arabidopsis thaliana OX=3702 GN=PUB43 PE=2 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 8.9e-39
Identity = 192/730 (26.30%), Postives = 335/730 (45.89%), Query Frame = 0

Query: 138 NCSPTSTSSRTTEFICDLRVEEIEEE---RTSVKACDIALHLKY-GN-DDEFKLAVAGLK 197
           +C  TS     T+    + +    EE   R      DIA    Y GN +    LA+  ++
Sbjct: 70  SCPITSKELSITDLSPSIALRNTIEEWRARNDALKLDIARQSLYLGNAETNILLALKNVR 129

Query: 198 DLIQNKNVDDGWLNEEGIVSILLNRLGSNKSVNRPLIIQVLRYLVWNNPASKEMMADVGP 257
           ++ +N       +    +V ++ + L S+    R   +Q L+ +V  +  SK ++A+   
Sbjct: 130 EICRNIRKIRQRVCNPQLVRLITDMLKSSSHEVRCKALQTLQVVVEGDEESKAIVAEGDT 189

Query: 258 LSTLVKSLAGDEEERRE-VVGLLLELCDFVNVRRRLGRIQGCIVMLVSIL--KGDDQIAS 317
           + T+VK L+ +  + RE  V +L EL     +  ++G I G I++LV +   K ++    
Sbjct: 190 VRTIVKFLSQEPSKGREAAVSVLFELSKSEALCEKIGSIHGAIILLVGLTSSKSENVSTV 249

Query: 318 YDARKLLNVLSGNTQNVLYMAEAGYFKPMVQHLIEGSDMNKILMATGLSRMEHTEQSKAS 377
             A K L  L  + +NV  MA  G  +P++  L+EGS   K+ MA  L  +      K  
Sbjct: 250 EKADKTLTNLERSEENVRQMAINGRLQPLLAKLLEGSPETKVSMAFYLGVLALNNDVKVI 309

Query: 378 LGEEGVIEPLVQMFRTEKLEAKLSALSALQSLSGLKENVQRLISSGIVVSLLQLLFSV-- 437
           + +  V   L+ + RT  +  + +AL AL ++S  + + + LI++GI+  L++ LF V  
Sbjct: 310 VAQT-VGSSLIDLMRTRDMSQREAALGALNNISSFEGSAKLLINTGILPPLIKDLFYVGP 369

Query: 438 TSVLMTLREPAAAILAKI------------SESESILTNYDVALQMLSLLNLSSPIIQNH 497
             + + L+E +A ILA I                  L + ++   +L L + + P IQ  
Sbjct: 370 NQLPIRLKEVSATILANIVNIGYDFDKVPVGPHHQTLVSEEIVENLLQLTSNTGPEIQGK 429

Query: 498 LLQALNNIAANP-SALEVRKKMVESGAIQLLFPFL-MEDNTKVKSGALKLLYTLSKDAPE 557
           LL  L  + + P S + V   +  S AI  L  F+ + +N  ++  ++KLL+ +S    E
Sbjct: 430 LLAVLVGLTSCPNSVINVVSAIRNSAAIISLVQFVEIHENDDLRLASIKLLHNISPHMSE 489

Query: 558 ELEES------HISLILNIIS--STDCKSERVFAVGILSNVPVTQKKIT-DLLRKANLVP 617
           EL  +       +  +++IIS  +     E+  A G+L+ +P     +T  LLR+     
Sbjct: 490 ELANALRSTVGQLGSLVSIISENTPTITEEQAAAAGLLAELPERDLVLTMRLLREGAFEK 549

Query: 618 ILISI--MNSSSANSDIFASLSSESVAGLLVRFTNPFDRKLQ--LHSVEQGVIPLLVKLL 677
           I+  I  +         F     E +  +L R T    ++    L   E+ +  L + LL
Sbjct: 550 IISKIVGIRQGEIRGIRFERTFLEGLVSILARITFALTKETDATLFCCEKNLPSLFLDLL 609

Query: 678 SSES-PVAQSKAAISLAQLSQNSLSLSKSR--------TSRWLCV--PPSKDSICEVHGR 737
            S S    Q  +A +L  LS  S +L+K           S + C+  PP    IC++H  
Sbjct: 610 QSNSQDNIQRASATALENLSLESKNLTKIPELPPPTYCVSIFSCLSKPPVVLGICKIHQG 669

Query: 738 QCLTKRTFCLVKADAIRPMIEILEGKESEVDEAVLSALTTLLEDEI-CDNGSNYVVKMSG 797
            C  + +FCLV+  A+  ++++L+ +  +V    L+AL+TLLED +    G   + +  G
Sbjct: 670 ICSVRESFCLVEGQAVDKLVDLLDHENDKVVGPALAALSTLLEDGLDVVQGVRLIDEADG 729

Query: 798 VEAILKVLGSGHID-AQQKALWILERIFRIEEHRVKYGE--TAWSILVDLSQKGDSSLKS 816
           +  IL VL     +  + +A+W++ERI RIEE   + GE     + LVD  Q  D   + 
Sbjct: 730 ITPILNVLLENRTENLRIRAVWMVERILRIEEIAREVGEEQNVTAALVDAFQNADFRTRQ 789

BLAST of Cucsat.G4436 vs. ExPASy Swiss-Prot
Match: Q9CAA7 (Putative U-box domain-containing protein 42 OS=Arabidopsis thaliana OX=3702 GN=PUB42 PE=3 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 1.0e-34
Identity = 184/747 (24.63%), Postives = 339/747 (45.38%), Query Frame = 0

Query: 136  DENCSPTSTSSRTTEFICDLRVE------EIEEERTSVKACDIALHLKYGNDDEFKLAVA 195
            DE   P +    TTE   ++ ++      ++  E   +K    AL L  G++     A+ 
Sbjct: 287  DEINCPVTGQKLTTELSANVVLKTIIQEWKVRNEAARIKVAHAALSLG-GSESMVIDALR 346

Query: 196  GLKDLIQNKNVDDGWLNEEGIVSILLNRLGSNKSVNRPLIIQVLRYLV-WNNPASKEMMA 255
             L+   + K  +   + E GI+ +L   L       R  +++ LR L        KEM+ 
Sbjct: 347  DLQMTCEGKEYNKVQVREAGIIQLLDRYLTYRSKDVRFELLKFLRTLADEETDDGKEMIV 406

Query: 256  DVGPLSTLVKSLAGDEEE-RREVVGLLLELCDFVNVRRRLGRIQGCIVMLVSIL--KGDD 315
                +S ++K L    +  R     LLLEL    +   ++G  +G I+MLV+    +  D
Sbjct: 407  KTITMSCVIKLLGSSHQPVRHAAQALLLELSKSQHACEKIGTARGAILMLVTAKYNRELD 466

Query: 316  QIASYDARKLLNVLSGNTQNVLYMAEAGYFKPMVQHLIEGSDMNKILMATGLSRMEHTEQ 375
              AS  + ++L  L    +N+  MAE+G  +P++ HL EGS+  ++ MA  L  ++   +
Sbjct: 467  SFASETSDQILRNLEKCPENIKQMAESGLLEPLLGHLAEGSEETQVAMAAYLVEIDIGHE 526

Query: 376  SKASLGEEGVIEPLVQMFRTEKLEAKLSALSALQSLSGLKENVQRLISSGIVVSLLQLLF 435
             K  + E+     L+ + ++E ++A+ +A  AL  +S    N + L+  GI+  +++ +F
Sbjct: 527  KKTYVAEK-ACPALIGLVQSENIDARRAAFKALAHISLYHPNNKILVEVGIIKIMVEEMF 586

Query: 436  S--VTSVLMTLREPAAAILAKISESESILTNYDV------------ALQMLSLLNLSSPI 495
            +  V S LM  R  AA ILA I ES      ++V               ++ +L  SSP 
Sbjct: 587  TKRVFSDLMNSRNEAATILANILESGLEHETFEVNTHGHTLGSDYFVYNIIHMLKNSSPD 646

Query: 496  IQN-HLLQALNNIAANPSALEVRKKMV-ESGAIQLLFPFLMEDNTKVKSGALKLLYTLSK 555
              N  L++ L +++ +P A+     ++ E+ A   +   +   + ++  GALKLL  L+ 
Sbjct: 647  DLNIDLIRILLSLSKSPRAMATIVSVIKETDASFAMIELINNPHDELGVGALKLLIALTP 706

Query: 556  DAPEELEE-------SHISLILNIISSTDCKSERVFAVGILSNVPVTQKKITDLLRKANL 615
                 L E          +LI   + +     +   +  +L+ +P     +   L   ++
Sbjct: 707  YIGHTLSERLCKTRGQPENLIQCPVEANQITEKHAVSAKLLAKLPHQNLTLNLALVNESI 766

Query: 616  VPIL---ISIMNSSSANSDIFASLSSESVAGLLVRFTNPFDRKLQL-----HSVEQGVIP 675
            V  +   I ++  S A +  +A+   E + G+LVRFT        +     H +    + 
Sbjct: 767  VSEILHAIHLIQRSGARTSRYATDFLEGLVGILVRFTTTLYEPQMMYLARNHDLTSVFVD 826

Query: 676  LLVKLLSSESPVAQSKAAISLAQLSQNSLSLSK---SRTSRW---LCVPPS--------- 735
            LL+K  S E    Q  +A  L  LS  +++LS+    R++++   L +P S         
Sbjct: 827  LLMKTSSDE---VQRLSATGLENLSSTTMTLSRPPQPRSTKFMGSLSMPRSFSLRSSKKK 886

Query: 736  KDSICEVHGRQCLTKRTFCLVKADAIRPMIEILEGKESEVDEAVLSALTTLLEDEI-CDN 795
            +  IC +H   C  K TFCLV+A+AI  ++  L+  + EV E+ L+A+ TLL+D++  + 
Sbjct: 887  QIEICAIHRGVCSAKNTFCLVEANAITKLLACLQSDKVEVVESALAAICTLLDDKVEVEK 946

Query: 796  GSNYVVKMSGVEAILKVLGSGHIDA-QQKALWILERIF---------RIEEHRVKYGETA 816
              + + +M+ V+ IL  +     ++  QKA W++++            I + R+  G   
Sbjct: 947  SLSMLSEMNAVQLILNAVKEHKKESLLQKAFWMIDKFIIRGGDKYASEISQDRMLSG--- 1006

BLAST of Cucsat.G4436 vs. ExPASy Swiss-Prot
Match: O22193 (U-box domain-containing protein 4 OS=Arabidopsis thaliana OX=3702 GN=PUB4 PE=1 SV=3)

HSP 1 Score: 63.2 bits (152), Expect = 1.6e-08
Identity = 60/210 (28.57%), Postives = 96/210 (45.71%), Query Frame = 0

Query: 243 SKEMMADVGPLSTLVKSLAGD------EEERREVVGLLLELCDFVNVRRRLGRIQGCIVM 302
           S E   D+  + T VK L  +      + +R+    L L     ++ R  +G   G IV+
Sbjct: 529 SNETRRDLSEVETQVKKLVEELKSSSLDTQRQATAELRLLAKHNMDNRIVIGN-SGAIVL 588

Query: 303 LVSILKGDDQIASYDARKLLNVLSGNTQNVLYMAEAGYFKPMVQHLIEGSDMNKILMATG 362
           LV +L   D     +A   L  LS N  N   +A+AG  +P++  L  GS   K   A  
Sbjct: 589 LVELLYSTDSATQENAVTALLNLSINDNNKKAIADAGAIEPLIHVLENGSSEAKENSAAT 648

Query: 363 LSRMEHTEQSKASLGEEGVIEPLVQMFRTEKLEAKLSALSALQSLSGLKENVQRLISSGI 422
           L  +   E++K  +G+ G I PLV +        K  A +AL +LS  +EN   ++ SG 
Sbjct: 649 LFSLSVIEENKIKIGQSGAIGPLVDLLGNGTPRGKKDAATALFNLSIHQENKAMIVQSGA 708

Query: 423 VVSLLQLLFSVTSVLMTLREPAAAILAKIS 447
           V  L+ L+     ++    + A A+LA ++
Sbjct: 709 VRYLIDLMDPAAGMV----DKAVAVLANLA 733

BLAST of Cucsat.G4436 vs. ExPASy Swiss-Prot
Match: Q5VRH9 (U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB12 PE=2 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 4.2e-04
Identity = 51/221 (23.08%), Postives = 99/221 (44.80%), Query Frame = 0

Query: 251 GPLSTLVKSLAGDEEERREVVGLLLELCDFVNVRRRLGRIQ-GCIVMLVSILKGDDQIAS 310
           G +S + +  +G+++E+R   G  + L    NV  R+   + G I +LV++L   D    
Sbjct: 324 GLVSLMNRLRSGNQDEQRAAAG-EIRLLAKRNVNNRICIAEAGAIPLLVNLLSSSDPRTQ 383

Query: 311 YDARKLLNVLSGNTQNVLYMAEAGYFKPMVQHLIEGSDMNKILMATGLSRMEHTEQSKAS 370
             A   L  LS +  N   + ++     +V+ L  GS   +   A  L  +   +++K +
Sbjct: 384 EHAVTALLNLSIHENNKASIVDSHAIPKIVEVLKTGSMETRENAAATLFSLSVVDENKVT 443

Query: 371 LGEEGVIEPLVQMFRTEKLEAKLSALSALQSLSGLKENVQRLISSGIVVSLLQLLFSVTS 430
           +G  G I PL+ +        K  A +A+ +L   + N  R + +GIV+ L+  L   T 
Sbjct: 444 IGAAGAIPPLINLLCDGSPRGKKDAATAIFNLCIYQGNKVRAVKAGIVIHLMNFLVDPTG 503

Query: 431 VLMTLREPAAAILAKISESESILTNYDVALQMLSLLNLSSP 471
            ++       +ILA   E + ++   +    ++ ++   SP
Sbjct: 504 GMIDEALSLLSILAGNPEGKIVIARSEPIPPLVEVIKTGSP 543

BLAST of Cucsat.G4436 vs. NCBI nr
Match: XP_011654477.1 (U-box domain-containing protein 44 [Cucumis sativus] >KGN49633.1 hypothetical protein Csa_018414 [Cucumis sativus])

HSP 1 Score: 1540 bits (3987), Expect = 0.0
Identity = 825/825 (100.00%), Postives = 825/825 (100.00%), Query Frame = 0

Query: 1   MKEMENRTFSEVVSEIIASTDELASISKNSETENEMFTELALVLEKIPPIFNDLRDYDKI 60
           MKEMENRTFSEVVSEIIASTDELASISKNSETENEMFTELALVLEKIPPIFNDLRDYDKI
Sbjct: 1   MKEMENRTFSEVVSEIIASTDELASISKNSETENEMFTELALVLEKIPPIFNDLRDYDKI 60

Query: 61  VDTPTIRKAVESLEKEIKRAKCSIKVHNQKVKHVESIAHDLGRSLGLVLFATVEVSTQFK 120
           VDTPTIRKAVESLEKEIKRAKCSIKVHNQKVKHVESIAHDLGRSLGLVLFATVEVSTQFK
Sbjct: 61  VDTPTIRKAVESLEKEIKRAKCSIKVHNQKVKHVESIAHDLGRSLGLVLFATVEVSTQFK 120

Query: 121 TKIGELHKELMNMKFDENCSPTSTSSRTTEFICDLRVEEIEEERTSVKACDIALHLKYGN 180
           TKIGELHKELMNMKFDENCSPTSTSSRTTEFICDLRVEEIEEERTSVKACDIALHLKYGN
Sbjct: 121 TKIGELHKELMNMKFDENCSPTSTSSRTTEFICDLRVEEIEEERTSVKACDIALHLKYGN 180

Query: 181 DDEFKLAVAGLKDLIQNKNVDDGWLNEEGIVSILLNRLGSNKSVNRPLIIQVLRYLVWNN 240
           DDEFKLAVAGLKDLIQNKNVDDGWLNEEGIVSILLNRLGSNKSVNRPLIIQVLRYLVWNN
Sbjct: 181 DDEFKLAVAGLKDLIQNKNVDDGWLNEEGIVSILLNRLGSNKSVNRPLIIQVLRYLVWNN 240

Query: 241 PASKEMMADVGPLSTLVKSLAGDEEERREVVGLLLELCDFVNVRRRLGRIQGCIVMLVSI 300
           PASKEMMADVGPLSTLVKSLAGDEEERREVVGLLLELCDFVNVRRRLGRIQGCIVMLVSI
Sbjct: 241 PASKEMMADVGPLSTLVKSLAGDEEERREVVGLLLELCDFVNVRRRLGRIQGCIVMLVSI 300

Query: 301 LKGDDQIASYDARKLLNVLSGNTQNVLYMAEAGYFKPMVQHLIEGSDMNKILMATGLSRM 360
           LKGDDQIASYDARKLLNVLSGNTQNVLYMAEAGYFKPMVQHLIEGSDMNKILMATGLSRM
Sbjct: 301 LKGDDQIASYDARKLLNVLSGNTQNVLYMAEAGYFKPMVQHLIEGSDMNKILMATGLSRM 360

Query: 361 EHTEQSKASLGEEGVIEPLVQMFRTEKLEAKLSALSALQSLSGLKENVQRLISSGIVVSL 420
           EHTEQSKASLGEEGVIEPLVQMFRTEKLEAKLSALSALQSLSGLKENVQRLISSGIVVSL
Sbjct: 361 EHTEQSKASLGEEGVIEPLVQMFRTEKLEAKLSALSALQSLSGLKENVQRLISSGIVVSL 420

Query: 421 LQLLFSVTSVLMTLREPAAAILAKISESESILTNYDVALQMLSLLNLSSPIIQNHLLQAL 480
           LQLLFSVTSVLMTLREPAAAILAKISESESILTNYDVALQMLSLLNLSSPIIQNHLLQAL
Sbjct: 421 LQLLFSVTSVLMTLREPAAAILAKISESESILTNYDVALQMLSLLNLSSPIIQNHLLQAL 480

Query: 481 NNIAANPSALEVRKKMVESGAIQLLFPFLMEDNTKVKSGALKLLYTLSKDAPEELEESHI 540
           NNIAANPSALEVRKKMVESGAIQLLFPFLMEDNTKVKSGALKLLYTLSKDAPEELEESHI
Sbjct: 481 NNIAANPSALEVRKKMVESGAIQLLFPFLMEDNTKVKSGALKLLYTLSKDAPEELEESHI 540

Query: 541 SLILNIISSTDCKSERVFAVGILSNVPVTQKKITDLLRKANLVPILISIMNSSSANSDIF 600
           SLILNIISSTDCKSERVFAVGILSNVPVTQKKITDLLRKANLVPILISIMNSSSANSDIF
Sbjct: 541 SLILNIISSTDCKSERVFAVGILSNVPVTQKKITDLLRKANLVPILISIMNSSSANSDIF 600

Query: 601 ASLSSESVAGLLVRFTNPFDRKLQLHSVEQGVIPLLVKLLSSESPVAQSKAAISLAQLSQ 660
           ASLSSESVAGLLVRFTNPFDRKLQLHSVEQGVIPLLVKLLSSESPVAQSKAAISLAQLSQ
Sbjct: 601 ASLSSESVAGLLVRFTNPFDRKLQLHSVEQGVIPLLVKLLSSESPVAQSKAAISLAQLSQ 660

Query: 661 NSLSLSKSRTSRWLCVPPSKDSICEVHGRQCLTKRTFCLVKADAIRPMIEILEGKESEVD 720
           NSLSLSKSRTSRWLCVPPSKDSICEVHGRQCLTKRTFCLVKADAIRPMIEILEGKESEVD
Sbjct: 661 NSLSLSKSRTSRWLCVPPSKDSICEVHGRQCLTKRTFCLVKADAIRPMIEILEGKESEVD 720

Query: 721 EAVLSALTTLLEDEICDNGSNYVVKMSGVEAILKVLGSGHIDAQQKALWILERIFRIEEH 780
           EAVLSALTTLLEDEICDNGSNYVVKMSGVEAILKVLGSGHIDAQQKALWILERIFRIEEH
Sbjct: 721 EAVLSALTTLLEDEICDNGSNYVVKMSGVEAILKVLGSGHIDAQQKALWILERIFRIEEH 780

Query: 781 RVKYGETAWSILVDLSQKGDSSLKSTIAKLLVRLELFQFQHSEYL 825
           RVKYGETAWSILVDLSQKGDSSLKSTIAKLLVRLELFQFQHSEYL
Sbjct: 781 RVKYGETAWSILVDLSQKGDSSLKSTIAKLLVRLELFQFQHSEYL 825

BLAST of Cucsat.G4436 vs. NCBI nr
Match: XP_008450258.1 (PREDICTED: U-box domain-containing protein 44-like [Cucumis melo] >KAA0058419.1 U-box domain-containing protein 44-like [Cucumis melo var. makuwa] >TYK15339.1 U-box domain-containing protein 44-like [Cucumis melo var. makuwa])

HSP 1 Score: 1482 bits (3836), Expect = 0.0
Identity = 791/825 (95.88%), Postives = 807/825 (97.82%), Query Frame = 0

Query: 1   MKEMENRTFSEVVSEIIASTDELASISKNSETENEMFTELALVLEKIPPIFNDLRDYDKI 60
           MKEM+NRTFSEVVSEIIASTDELASISKNSETE EMFTELALVLEKIPPIFNDLRD DKI
Sbjct: 1   MKEMQNRTFSEVVSEIIASTDELASISKNSETEKEMFTELALVLEKIPPIFNDLRDCDKI 60

Query: 61  VDTPTIRKAVESLEKEIKRAKCSIKVHNQKVKHVESIAHDLGRSLGLVLFATVEVSTQFK 120
           +DTPTIRKAVESLEKEIKRAKCSIKVHNQKVKHVESIAHDLGRSLGLVLFATVEVSTQFK
Sbjct: 61  MDTPTIRKAVESLEKEIKRAKCSIKVHNQKVKHVESIAHDLGRSLGLVLFATVEVSTQFK 120

Query: 121 TKIGELHKELMNMKFDENCSPTSTSSRTTEFICDLRVEEIEEERTSVKACDIALHLKYGN 180
           TKIGELHKELM MKFDENCSPTSTSSRTTEFICDLRVEEIEEER S+KACDIALHLKYG 
Sbjct: 121 TKIGELHKELMKMKFDENCSPTSTSSRTTEFICDLRVEEIEEERNSIKACDIALHLKYGK 180

Query: 181 DDEFKLAVAGLKDLIQNKNVDDGWLNEEGIVSILLNRLGSNKSVNRPLIIQVLRYLVWNN 240
           DDEFK AV GLK+LIQ+KNVDDGWLNEEGIVSILLNRLG NK +NRP+IIQVLR LVWNN
Sbjct: 181 DDEFKRAVVGLKELIQSKNVDDGWLNEEGIVSILLNRLGCNKFINRPIIIQVLRCLVWNN 240

Query: 241 PASKEMMADVGPLSTLVKSLAGDEEERREVVGLLLELCDFVNVRRRLGRIQGCIVMLVSI 300
           PA+KEMMADVG LSTLVKSLAGDEEERREVVGLLLELCD VNVRRRLGRIQGCIVMLVSI
Sbjct: 241 PANKEMMADVGSLSTLVKSLAGDEEERREVVGLLLELCDLVNVRRRLGRIQGCIVMLVSI 300

Query: 301 LKGDDQIASYDARKLLNVLSGNTQNVLYMAEAGYFKPMVQHLIEGSDMNKILMATGLSRM 360
           LKGDDQIASYDARKLLNVLSGNTQNVLYMAEAGYFKPMVQHLIEGSDMNKILMATGLSRM
Sbjct: 301 LKGDDQIASYDARKLLNVLSGNTQNVLYMAEAGYFKPMVQHLIEGSDMNKILMATGLSRM 360

Query: 361 EHTEQSKASLGEEGVIEPLVQMFRTEKLEAKLSALSALQSLSGLKENVQRLISSGIVVSL 420
           EHTEQSKASLGEEGVIEPLVQMFRTEKLEAKLSALSALQSLSGLKEN+QRLISSGIVVSL
Sbjct: 361 EHTEQSKASLGEEGVIEPLVQMFRTEKLEAKLSALSALQSLSGLKENIQRLISSGIVVSL 420

Query: 421 LQLLFSVTSVLMTLREPAAAILAKISESESILTNYDVALQMLSLLNLSSPIIQNHLLQAL 480
           LQLLFSVTSVLMTLREPAAAILAKISESESILTNYDVALQMLSLLNLSSPIIQNHLLQAL
Sbjct: 421 LQLLFSVTSVLMTLREPAAAILAKISESESILTNYDVALQMLSLLNLSSPIIQNHLLQAL 480

Query: 481 NNIAANPSALEVRKKMVESGAIQLLFPFLMEDNTKVKSGALKLLYTLSKDAPEELEESHI 540
           NNIAANPSALEVRKKMVESGAIQLLFPFLMEDN KVKSGALKLLYTLSKDAPEELEESHI
Sbjct: 481 NNIAANPSALEVRKKMVESGAIQLLFPFLMEDNAKVKSGALKLLYTLSKDAPEELEESHI 540

Query: 541 SLILNIISSTDCKSERVFAVGILSNVPVTQKKITDLLRKANLVPILISIMNSSSANSDIF 600
            +ILNIISSTDCKSERVFAVGILSNVPVTQKKITDLLRKANLVPILISIMNSS++ SD+F
Sbjct: 541 CVILNIISSTDCKSERVFAVGILSNVPVTQKKITDLLRKANLVPILISIMNSSTSKSDVF 600

Query: 601 ASLSSESVAGLLVRFTNPFDRKLQLHSVEQGVIPLLVKLLSSESPVAQSKAAISLAQLSQ 660
           ASLSSESVAGLLVRFTNPFDRKLQLHSVEQGVIPLLVKLLSSES VAQSKAAISLAQLSQ
Sbjct: 601 ASLSSESVAGLLVRFTNPFDRKLQLHSVEQGVIPLLVKLLSSESHVAQSKAAISLAQLSQ 660

Query: 661 NSLSLSKSRTSRWLCVPPSKDSICEVHGRQCLTKRTFCLVKADAIRPMIEILEGKESEVD 720
           NSLSLSKSRTSRWLCVPPSKDSICEVHGRQC  KRTFCLVKADAIRPMIEILEGKESEVD
Sbjct: 661 NSLSLSKSRTSRWLCVPPSKDSICEVHGRQCFIKRTFCLVKADAIRPMIEILEGKESEVD 720

Query: 721 EAVLSALTTLLEDEICDNGSNYVVKMSGVEAILKVLGSGHIDAQQKALWILERIFRIEEH 780
           EAVLSALTTLLEDEICDNGSNYVVKMSGV+AILKVLGSGHID+QQKALWILERIFRIEEH
Sbjct: 721 EAVLSALTTLLEDEICDNGSNYVVKMSGVQAILKVLGSGHIDSQQKALWILERIFRIEEH 780

Query: 781 RVKYGETAWSILVDLSQKGDSSLKSTIAKLLVRLELFQFQHSEYL 825
           RVKYGETAWS+LVDLSQKGDSSLKSTIAKLLVRLELFQFQHSEYL
Sbjct: 781 RVKYGETAWSVLVDLSQKGDSSLKSTIAKLLVRLELFQFQHSEYL 825

BLAST of Cucsat.G4436 vs. NCBI nr
Match: XP_038892373.1 (U-box domain-containing protein 44-like [Benincasa hispida])

HSP 1 Score: 1405 bits (3637), Expect = 0.0
Identity = 748/822 (91.00%), Postives = 786/822 (95.62%), Query Frame = 0

Query: 1   MKEMENRTFSEVVSEIIASTDELASISKNSETENEMFTELALVLEKIPPIFNDLRDYDKI 60
           +KEMENRTFSEVVS+IIASTDELASISK+SETE EMFTELALVLEKIPPIFNDLRDYDKI
Sbjct: 10  LKEMENRTFSEVVSQIIASTDELASISKSSETEKEMFTELALVLEKIPPIFNDLRDYDKI 69

Query: 61  VDTPTIRKAVESLEKEIKRAKCSIKVHNQKVKHVESIAHDLGRSLGLVLFATVEVSTQFK 120
           +DTP+IRKAVESLEKEIKRAK  IKVHNQK+KHVESIAHDLGRSLGLVLFATVEVSTQFK
Sbjct: 70  MDTPSIRKAVESLEKEIKRAKSLIKVHNQKMKHVESIAHDLGRSLGLVLFATVEVSTQFK 129

Query: 121 TKIGELHKELMNMKFDENCSPTSTS--SRTTEFICDLRVEEIEEERTSVKACDIALHLKY 180
           TKIGELHKELMNMKF+ENCSPTSTS  SRTTEFICDLRVEEIEEER S+K CDIALHLKY
Sbjct: 130 TKIGELHKELMNMKFNENCSPTSTSTSSRTTEFICDLRVEEIEEERISIKVCDIALHLKY 189

Query: 181 GNDDEFKLAVAGLKDLIQNKNVDDGWLNEEGIVSILLNRLGSNKSVNRPLIIQVLRYLVW 240
           GNDDE K AV GLK+LIQ+KN++D WLNEEGI+SILLNRLGS+KS NR +IIQVLRYLVW
Sbjct: 190 GNDDEVKRAVVGLKELIQSKNINDEWLNEEGIISILLNRLGSSKSTNRLIIIQVLRYLVW 249

Query: 241 NNPASKEMMADVGPLSTLVKSLAGDEEERREVVGLLLELCDFVNVRRRLGRIQGCIVMLV 300
           N+PASKEMMADVGPLSTLVKSLAGDEEERRE VGLLLELCD VNVRRRLGR+QGCIVMLV
Sbjct: 250 NSPASKEMMADVGPLSTLVKSLAGDEEERREAVGLLLELCDLVNVRRRLGRVQGCIVMLV 309

Query: 301 SILKGDDQIASYDARKLLNVLSGNTQNVLYMAEAGYFKPMVQHLIEGSDMNKILMATGLS 360
           +ILKGDDQIAS DARKLLNVLSGNTQNVLYMAEAGYFKPMVQHLIEGS+MNKILMAT LS
Sbjct: 310 AILKGDDQIASSDARKLLNVLSGNTQNVLYMAEAGYFKPMVQHLIEGSNMNKILMATALS 369

Query: 361 RMEHTEQSKASLGEEGVIEPLVQMFRTEKLEAKLSALSALQSLSGLKENVQRLISSGIVV 420
           RMEHTEQSKASLGEEG IEPLVQMFRTEKLEAKLSALSALQSLSGL+ENVQRLISSGIV+
Sbjct: 370 RMEHTEQSKASLGEEGAIEPLVQMFRTEKLEAKLSALSALQSLSGLQENVQRLISSGIVI 429

Query: 421 SLLQLLFSVTSVLMTLREPAAAILAKISESESILTNYDVALQMLSLLNLSSPIIQNHLLQ 480
            LLQLLFSVTSVLMTLREPAAAILAKISESESIL N+DVALQMLSLLNLSSP+IQNHLLQ
Sbjct: 430 LLLQLLFSVTSVLMTLREPAAAILAKISESESILMNHDVALQMLSLLNLSSPVIQNHLLQ 489

Query: 481 ALNNIAANPSALEVRKKMVESGAIQLLFPFLMEDNTKVKSGALKLLYTLSKDAPEELEES 540
           ALNNI ANP ALEVRKKMVESGAIQLLFPFLMEDN K+KSGALKLLYTL KDAPEELEES
Sbjct: 490 ALNNIVANPDALEVRKKMVESGAIQLLFPFLMEDNAKIKSGALKLLYTLLKDAPEELEES 549

Query: 541 HISLILNIISSTDCKSERVFAVGILSNVPVTQKKITDLLRKANLVPILISIMNSSSANSD 600
           HIS+ILNIISST+C SERVFAVGILSNVPVTQKKIT++LRKANLVPILISIMNS  ANSD
Sbjct: 550 HISVILNIISSTNCISERVFAVGILSNVPVTQKKITEMLRKANLVPILISIMNSGLANSD 609

Query: 601 IFASLSSESVAGLLVRFTNPFDRKLQLHSVEQGVIPLLVKLLSSESPVAQSKAAISLAQL 660
           +  SLSSESVAGLLVRFTNPFDRKLQLHS EQGVIPLLVKLLSSESP+AQSKAA SLAQL
Sbjct: 610 VSISLSSESVAGLLVRFTNPFDRKLQLHSAEQGVIPLLVKLLSSESPIAQSKAATSLAQL 669

Query: 661 SQNSLSLSKSRTSRWLCVPPSKDSICEVHGRQCLTKRTFCLVKADAIRPMIEILEGKESE 720
           SQNSLSLSKSRTSRWLC PPSKDSICEVHGRQC  K+TFCLVKADAI PMI+ILEGKESE
Sbjct: 670 SQNSLSLSKSRTSRWLCGPPSKDSICEVHGRQCFIKKTFCLVKADAIPPMIQILEGKESE 729

Query: 721 VDEAVLSALTTLLEDEICDNGSNYVVKMSGVEAILKVLGSGHIDAQQKALWILERIFRIE 780
           VDEAVLSALTTLLEDEICDNGS Y+VKMSG++AILKVLGSGH+DAQQKALWILERIFRIE
Sbjct: 730 VDEAVLSALTTLLEDEICDNGSKYIVKMSGIQAILKVLGSGHVDAQQKALWILERIFRIE 789

Query: 781 EHRVKYGETAWSILVDLSQKGDSSLKSTIAKLLVRLELFQFQ 820
           EHRV+YGETAWS+LVDLSQKGDSSLKSTIAKLLVRLELFQFQ
Sbjct: 790 EHRVQYGETAWSVLVDLSQKGDSSLKSTIAKLLVRLELFQFQ 831

BLAST of Cucsat.G4436 vs. NCBI nr
Match: XP_022138933.1 (U-box domain-containing protein 44-like [Momordica charantia] >XP_022138934.1 U-box domain-containing protein 44-like [Momordica charantia])

HSP 1 Score: 1311 bits (3393), Expect = 0.0
Identity = 699/820 (85.24%), Postives = 760/820 (92.68%), Query Frame = 0

Query: 1   MKEMENRTFSEVVSEIIASTDELASISKNSETENEMFTELALVLEKIPPIFNDLRDYDKI 60
           + ++ENRTFSEVVSEIIASTDELASISK+SETE EMF+ELALV+EKIPPIFNDLRDYDKI
Sbjct: 10  VNKIENRTFSEVVSEIIASTDELASISKSSETEKEMFSELALVVEKIPPIFNDLRDYDKI 69

Query: 61  VDTPTIRKAVESLEKEIKRAKCSIKVHNQKVKHVESIAHDLGRSLGLVLFATVEVSTQFK 120
           +DTP+IRKAVESLEKEIKRAKC I+VHNQK+KHVE+IAHDLGRSLGLVLFATVEVST+FK
Sbjct: 70  MDTPSIRKAVESLEKEIKRAKCLIEVHNQKMKHVETIAHDLGRSLGLVLFATVEVSTKFK 129

Query: 121 TKIGELHKELMNMKFDENCSPTSTSSRTTEFICDLRVEEIEEERTSVKACDIALHLKYGN 180
            KIGEL+KELM+MKF+ENCSPTSTSS+ TEF CDLRVEEIEEER S   CDIA+ LKYGN
Sbjct: 130 AKIGELYKELMSMKFNENCSPTSTSSQATEFGCDLRVEEIEEERISTNVCDIAVQLKYGN 189

Query: 181 DDEFKLAVAGLKDLIQNKNVDDGWLNEEGIVSILLNRLGSNKSVNRPLIIQVLRYLVWNN 240
           DDEFKLA  GLK+L+Q+KN+D+ WL EEGIVSILLNRLGS+KSV+R +IIQVL+Y+V N 
Sbjct: 190 DDEFKLAAMGLKELMQSKNIDEEWLKEEGIVSILLNRLGSSKSVDRSIIIQVLQYIVLNY 249

Query: 241 PASKEMMADVGPLSTLVKSLAGDEEERREVVGLLLELCDFVNVRRRLGRIQGCIVMLVSI 300
           PA KEMMADVGPLS L KSLAGDE ERRE VGLLL+LC+ VNVRRRLGR+QGC+VMLV+I
Sbjct: 250 PAIKEMMADVGPLSKLAKSLAGDEVERREAVGLLLDLCELVNVRRRLGRVQGCVVMLVAI 309

Query: 301 LKGDDQIASYDARKLLNVLSGNTQNVLYMAEAGYFKPMVQHLIEGSDMNKILMATGLSRM 360
           L GDDQIASYDARKLLNVLSGNTQNVLYMAEAGYF+PMVQHL EGSDMNKILMAT +SRM
Sbjct: 310 LNGDDQIASYDARKLLNVLSGNTQNVLYMAEAGYFRPMVQHLKEGSDMNKILMATAISRM 369

Query: 361 EHTEQSKASLGEEGVIEPLVQMFRTEKLEAKLSALSALQSLSGLKENVQRLISSGIVVSL 420
           E TEQS+ASLGEEG IEPLVQMF  EKLEAKLSALSALQSLS LKENVQRLI SGIV+SL
Sbjct: 370 ELTEQSRASLGEEGAIEPLVQMFWAEKLEAKLSALSALQSLSSLKENVQRLIGSGIVMSL 429

Query: 421 LQLLFSVTSVLMTLREPAAAILAKISESESILTNYDVALQMLSLLNLSSPIIQNHLLQAL 480
           LQLLFSVTSVLMTLREPAAAILAKI+ESESIL N+D+ALQMLSLLNLSSP+IQNHLLQAL
Sbjct: 430 LQLLFSVTSVLMTLREPAAAILAKIAESESILVNHDMALQMLSLLNLSSPVIQNHLLQAL 489

Query: 481 NNIAANPSALEVRKKMVESGAIQLLFPFLMEDNTKVKSGALKLLYTLSKDAPEELEESHI 540
           N IAA+PSA EVRKKMVESGAIQLLFPFLME N K+K+GAL LLY LSKDAPEEL ESHI
Sbjct: 490 NRIAAHPSAAEVRKKMVESGAIQLLFPFLMETNPKIKTGALNLLYNLSKDAPEELGESHI 549

Query: 541 SLILNIISSTDCKSERVFAVGILSNVPVTQKKITDLLRKANLVPILISIMNSSSANSDIF 600
           S+IL II+ST+ +SERVFAVGILSNVPVTQKKITD+LRKANLVP L+SIMNSS  NS+I 
Sbjct: 550 SVILEIITSTNSESERVFAVGILSNVPVTQKKITDMLRKANLVPTLVSIMNSSLTNSNIS 609

Query: 601 ASLSSESVAGLLVRFTNPFDRKLQLHSVEQGVIPLLVKLLSSESPVAQSKAAISLAQLSQ 660
            SL SESVAGLLVRFTNPFDRKLQLHS EQGVIPLLVKLLSS+SPVAQSKAA SLAQLSQ
Sbjct: 610 TSLLSESVAGLLVRFTNPFDRKLQLHSAEQGVIPLLVKLLSSDSPVAQSKAATSLAQLSQ 669

Query: 661 NSLSLSKSRTSRWLCVPPSKDSICEVHGRQCLTKRTFCLVKADAIRPMIEILEGKESEVD 720
           NSLSLSKSRTSRWLCVPPSKDSICEVHGRQC+ K TFCLVKA+A+ PMI+ILEGKE EVD
Sbjct: 670 NSLSLSKSRTSRWLCVPPSKDSICEVHGRQCIIKSTFCLVKANAVPPMIQILEGKEREVD 729

Query: 721 EAVLSALTTLLEDEICDNGSNYVVKMSGVEAILKVLGSGHIDAQQKALWILERIFRIEEH 780
           EAVLSALTTLLEDEICDNGSNY+VKMSGV+AILKVLGS HIDAQQKALWILERIFRIEEH
Sbjct: 730 EAVLSALTTLLEDEICDNGSNYIVKMSGVQAILKVLGSDHIDAQQKALWILERIFRIEEH 789

Query: 781 RVKYGETAWSILVDLSQKGDSSLKSTIAKLLVRLELFQFQ 820
           RV+YGE    +LVDL++KGDSSLKSTIAKLLVRLELFQFQ
Sbjct: 790 RVQYGEPTSFVLVDLAEKGDSSLKSTIAKLLVRLELFQFQ 829

BLAST of Cucsat.G4436 vs. NCBI nr
Match: XP_022993433.1 (U-box domain-containing protein 44-like [Cucurbita maxima])

HSP 1 Score: 1301 bits (3368), Expect = 0.0
Identity = 694/822 (84.43%), Postives = 756/822 (91.97%), Query Frame = 0

Query: 1   MKEMENRTFSEVVSEIIASTDELASISKNSETENEMFTELALVLEKIPPIFNDLRDYDKI 60
           +KE+ENRTFSEV+S IIASTDELASISK+SETE EMF ELALVLE+IPPIF+DLR+YDKI
Sbjct: 4   VKEIENRTFSEVISGIIASTDELASISKSSETEKEMFNELALVLERIPPIFSDLREYDKI 63

Query: 61  VDTPTIRKAVESLEKEIKRAKCSIKVHNQKVKHVESIAHDLGRSLGLVLFATVEVSTQFK 120
           +DTP+IRKAV SLEKEI RAKC I V NQK+KHVESIAHDLGRSLGLVLFAT EVSTQFK
Sbjct: 64  MDTPSIRKAVGSLEKEIMRAKCLINVPNQKMKHVESIAHDLGRSLGLVLFATAEVSTQFK 123

Query: 121 TKIGELHKELMNMKFDENCSPTSTS--SRTTEFICDLRVEEIEEERTSVKACDIALHLKY 180
            KIGELHKELMN+KF ENCSPTSTS  SRTTEF+CDLRVEEIEE++ S + CDIA HLKY
Sbjct: 124 AKIGELHKELMNLKFSENCSPTSTSTSSRTTEFVCDLRVEEIEEQQISTRVCDIAHHLKY 183

Query: 181 GNDDEFKLAVAGLKDLIQNKNVDDGWLNEEGIVSILLNRLGSNKSVNRPLIIQVLRYLVW 240
           GNDDEFKLAV GLK+LI NKNVDD WLNEEGIVSILLNR+ S+KS N+ +IIQVLRYLVW
Sbjct: 184 GNDDEFKLAVVGLKELIHNKNVDDEWLNEEGIVSILLNRMDSSKSANQSIIIQVLRYLVW 243

Query: 241 NNPASKEMMADVGPLSTLVKSLAGDEEERREVVGLLLELCDFVNVRRRLGRIQGCIVMLV 300
           N+PASK ++++VG LSTLVKSLAGDEEERRE VGLLLEL D VNVRRRLGR+QGCIVMLV
Sbjct: 244 NSPASKGLISNVGALSTLVKSLAGDEEERREAVGLLLELSDLVNVRRRLGRVQGCIVMLV 303

Query: 301 SILKGDDQIASYDARKLLNVLSGNTQNVLYMAEAGYFKPMVQHLIEGSDMNKILMATGLS 360
           +IL GDDQIAS +ARKLLNVLS NTQNVL+MAEAGYFKPMVQHL EGSDMNKILMATGLS
Sbjct: 304 AILNGDDQIASCNARKLLNVLSVNTQNVLHMAEAGYFKPMVQHLKEGSDMNKILMATGLS 363

Query: 361 RMEHTEQSKASLGEEGVIEPLVQMFRTEKLEAKLSALSALQSLSGLKENVQRLISSGIVV 420
           RMEHTEQSKASLGEEG IEPLVQMFRTEKLEAKLSALSALQSLSGL ENVQRLI SG+V+
Sbjct: 364 RMEHTEQSKASLGEEGAIEPLVQMFRTEKLEAKLSALSALQSLSGLNENVQRLIDSGMVI 423

Query: 421 SLLQLLFSVTSVLMTLREPAAAILAKISESESILTNYDVALQMLSLLNLSSPIIQNHLLQ 480
           SLLQLLFSVTSVLMTLREPAAAILA++SESES L N+++ALQMLSLLNLSSP+IQNHLLQ
Sbjct: 424 SLLQLLFSVTSVLMTLREPAAAILARMSESESFLMNHEMALQMLSLLNLSSPVIQNHLLQ 483

Query: 481 ALNNIAANPSALEVRKKMVESGAIQLLFPFLMEDNTKVKSGALKLLYTLSKDAPEELEES 540
           ALNNI AN SALEVR  MVE+GAIQLLFPF+ME+NTK+KSGALKLLYTLSKDAPEELEES
Sbjct: 484 ALNNIVANSSALEVRINMVENGAIQLLFPFVMENNTKIKSGALKLLYTLSKDAPEELEES 543

Query: 541 HISLILNIISSTDCKSERVFAVGILSNVPVTQKKITDLLRKANLVPILISIMNSSSANSD 600
            IS+IL+IISST CK+ERVFAVGILSNV VTQKK TD+LRKANL+PILISIMNSS ANSD
Sbjct: 544 QISVILSIISSTSCKAERVFAVGILSNVSVTQKKATDMLRKANLLPILISIMNSSLANSD 603

Query: 601 IFASLSSESVAGLLVRFTNPFDRKLQLHSVEQGVIPLLVKLLSSESPVAQSKAAISLAQL 660
           ++ SL SESVAGLLV+FTNPFDRKLQLHS EQGVIPLLVKLLSS+S +AQ KAA SLAQL
Sbjct: 604 VYVSLLSESVAGLLVQFTNPFDRKLQLHSAEQGVIPLLVKLLSSKSAIAQCKAATSLAQL 663

Query: 661 SQNSLSLSKSRTSRWLCVPPSKDSICEVHGRQCLTKRTFCLVKADAIRPMIEILEGKESE 720
           SQNSLSLSKSRT+RWLCVP SKDS+CEVHGRQC  K TFCLVKADAI PMI+ILEGKE E
Sbjct: 664 SQNSLSLSKSRTTRWLCVPYSKDSVCEVHGRQCFIKTTFCLVKADAIPPMIQILEGKERE 723

Query: 721 VDEAVLSALTTLLEDEICDNGSNYVVKMSGVEAILKVLGSGHIDAQQKALWILERIFRIE 780
           VDEAVLSALTTLLEDEICDNGS Y+VKM GV+ ILKVLGSGHID QQKALWILERIFRIE
Sbjct: 724 VDEAVLSALTTLLEDEICDNGSKYIVKMCGVQGILKVLGSGHIDTQQKALWILERIFRIE 783

Query: 781 EHRVKYGETAWSILVDLSQKGDSSLKSTIAKLLVRLELFQFQ 820
           EH V+YGETAWS+LVDLS+KGDSSL+S IAK+L +LELFQ Q
Sbjct: 784 EHGVQYGETAWSVLVDLSKKGDSSLESAIAKVLQQLELFQLQ 825

BLAST of Cucsat.G4436 vs. ExPASy TrEMBL
Match: A0A0A0KJA7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G034000 PE=4 SV=1)

HSP 1 Score: 1540 bits (3987), Expect = 0.0
Identity = 825/825 (100.00%), Postives = 825/825 (100.00%), Query Frame = 0

Query: 1   MKEMENRTFSEVVSEIIASTDELASISKNSETENEMFTELALVLEKIPPIFNDLRDYDKI 60
           MKEMENRTFSEVVSEIIASTDELASISKNSETENEMFTELALVLEKIPPIFNDLRDYDKI
Sbjct: 1   MKEMENRTFSEVVSEIIASTDELASISKNSETENEMFTELALVLEKIPPIFNDLRDYDKI 60

Query: 61  VDTPTIRKAVESLEKEIKRAKCSIKVHNQKVKHVESIAHDLGRSLGLVLFATVEVSTQFK 120
           VDTPTIRKAVESLEKEIKRAKCSIKVHNQKVKHVESIAHDLGRSLGLVLFATVEVSTQFK
Sbjct: 61  VDTPTIRKAVESLEKEIKRAKCSIKVHNQKVKHVESIAHDLGRSLGLVLFATVEVSTQFK 120

Query: 121 TKIGELHKELMNMKFDENCSPTSTSSRTTEFICDLRVEEIEEERTSVKACDIALHLKYGN 180
           TKIGELHKELMNMKFDENCSPTSTSSRTTEFICDLRVEEIEEERTSVKACDIALHLKYGN
Sbjct: 121 TKIGELHKELMNMKFDENCSPTSTSSRTTEFICDLRVEEIEEERTSVKACDIALHLKYGN 180

Query: 181 DDEFKLAVAGLKDLIQNKNVDDGWLNEEGIVSILLNRLGSNKSVNRPLIIQVLRYLVWNN 240
           DDEFKLAVAGLKDLIQNKNVDDGWLNEEGIVSILLNRLGSNKSVNRPLIIQVLRYLVWNN
Sbjct: 181 DDEFKLAVAGLKDLIQNKNVDDGWLNEEGIVSILLNRLGSNKSVNRPLIIQVLRYLVWNN 240

Query: 241 PASKEMMADVGPLSTLVKSLAGDEEERREVVGLLLELCDFVNVRRRLGRIQGCIVMLVSI 300
           PASKEMMADVGPLSTLVKSLAGDEEERREVVGLLLELCDFVNVRRRLGRIQGCIVMLVSI
Sbjct: 241 PASKEMMADVGPLSTLVKSLAGDEEERREVVGLLLELCDFVNVRRRLGRIQGCIVMLVSI 300

Query: 301 LKGDDQIASYDARKLLNVLSGNTQNVLYMAEAGYFKPMVQHLIEGSDMNKILMATGLSRM 360
           LKGDDQIASYDARKLLNVLSGNTQNVLYMAEAGYFKPMVQHLIEGSDMNKILMATGLSRM
Sbjct: 301 LKGDDQIASYDARKLLNVLSGNTQNVLYMAEAGYFKPMVQHLIEGSDMNKILMATGLSRM 360

Query: 361 EHTEQSKASLGEEGVIEPLVQMFRTEKLEAKLSALSALQSLSGLKENVQRLISSGIVVSL 420
           EHTEQSKASLGEEGVIEPLVQMFRTEKLEAKLSALSALQSLSGLKENVQRLISSGIVVSL
Sbjct: 361 EHTEQSKASLGEEGVIEPLVQMFRTEKLEAKLSALSALQSLSGLKENVQRLISSGIVVSL 420

Query: 421 LQLLFSVTSVLMTLREPAAAILAKISESESILTNYDVALQMLSLLNLSSPIIQNHLLQAL 480
           LQLLFSVTSVLMTLREPAAAILAKISESESILTNYDVALQMLSLLNLSSPIIQNHLLQAL
Sbjct: 421 LQLLFSVTSVLMTLREPAAAILAKISESESILTNYDVALQMLSLLNLSSPIIQNHLLQAL 480

Query: 481 NNIAANPSALEVRKKMVESGAIQLLFPFLMEDNTKVKSGALKLLYTLSKDAPEELEESHI 540
           NNIAANPSALEVRKKMVESGAIQLLFPFLMEDNTKVKSGALKLLYTLSKDAPEELEESHI
Sbjct: 481 NNIAANPSALEVRKKMVESGAIQLLFPFLMEDNTKVKSGALKLLYTLSKDAPEELEESHI 540

Query: 541 SLILNIISSTDCKSERVFAVGILSNVPVTQKKITDLLRKANLVPILISIMNSSSANSDIF 600
           SLILNIISSTDCKSERVFAVGILSNVPVTQKKITDLLRKANLVPILISIMNSSSANSDIF
Sbjct: 541 SLILNIISSTDCKSERVFAVGILSNVPVTQKKITDLLRKANLVPILISIMNSSSANSDIF 600

Query: 601 ASLSSESVAGLLVRFTNPFDRKLQLHSVEQGVIPLLVKLLSSESPVAQSKAAISLAQLSQ 660
           ASLSSESVAGLLVRFTNPFDRKLQLHSVEQGVIPLLVKLLSSESPVAQSKAAISLAQLSQ
Sbjct: 601 ASLSSESVAGLLVRFTNPFDRKLQLHSVEQGVIPLLVKLLSSESPVAQSKAAISLAQLSQ 660

Query: 661 NSLSLSKSRTSRWLCVPPSKDSICEVHGRQCLTKRTFCLVKADAIRPMIEILEGKESEVD 720
           NSLSLSKSRTSRWLCVPPSKDSICEVHGRQCLTKRTFCLVKADAIRPMIEILEGKESEVD
Sbjct: 661 NSLSLSKSRTSRWLCVPPSKDSICEVHGRQCLTKRTFCLVKADAIRPMIEILEGKESEVD 720

Query: 721 EAVLSALTTLLEDEICDNGSNYVVKMSGVEAILKVLGSGHIDAQQKALWILERIFRIEEH 780
           EAVLSALTTLLEDEICDNGSNYVVKMSGVEAILKVLGSGHIDAQQKALWILERIFRIEEH
Sbjct: 721 EAVLSALTTLLEDEICDNGSNYVVKMSGVEAILKVLGSGHIDAQQKALWILERIFRIEEH 780

Query: 781 RVKYGETAWSILVDLSQKGDSSLKSTIAKLLVRLELFQFQHSEYL 825
           RVKYGETAWSILVDLSQKGDSSLKSTIAKLLVRLELFQFQHSEYL
Sbjct: 781 RVKYGETAWSILVDLSQKGDSSLKSTIAKLLVRLELFQFQHSEYL 825

BLAST of Cucsat.G4436 vs. ExPASy TrEMBL
Match: A0A5D3CYU0 (U-box domain-containing protein 44-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold551G00010 PE=4 SV=1)

HSP 1 Score: 1482 bits (3836), Expect = 0.0
Identity = 791/825 (95.88%), Postives = 807/825 (97.82%), Query Frame = 0

Query: 1   MKEMENRTFSEVVSEIIASTDELASISKNSETENEMFTELALVLEKIPPIFNDLRDYDKI 60
           MKEM+NRTFSEVVSEIIASTDELASISKNSETE EMFTELALVLEKIPPIFNDLRD DKI
Sbjct: 1   MKEMQNRTFSEVVSEIIASTDELASISKNSETEKEMFTELALVLEKIPPIFNDLRDCDKI 60

Query: 61  VDTPTIRKAVESLEKEIKRAKCSIKVHNQKVKHVESIAHDLGRSLGLVLFATVEVSTQFK 120
           +DTPTIRKAVESLEKEIKRAKCSIKVHNQKVKHVESIAHDLGRSLGLVLFATVEVSTQFK
Sbjct: 61  MDTPTIRKAVESLEKEIKRAKCSIKVHNQKVKHVESIAHDLGRSLGLVLFATVEVSTQFK 120

Query: 121 TKIGELHKELMNMKFDENCSPTSTSSRTTEFICDLRVEEIEEERTSVKACDIALHLKYGN 180
           TKIGELHKELM MKFDENCSPTSTSSRTTEFICDLRVEEIEEER S+KACDIALHLKYG 
Sbjct: 121 TKIGELHKELMKMKFDENCSPTSTSSRTTEFICDLRVEEIEEERNSIKACDIALHLKYGK 180

Query: 181 DDEFKLAVAGLKDLIQNKNVDDGWLNEEGIVSILLNRLGSNKSVNRPLIIQVLRYLVWNN 240
           DDEFK AV GLK+LIQ+KNVDDGWLNEEGIVSILLNRLG NK +NRP+IIQVLR LVWNN
Sbjct: 181 DDEFKRAVVGLKELIQSKNVDDGWLNEEGIVSILLNRLGCNKFINRPIIIQVLRCLVWNN 240

Query: 241 PASKEMMADVGPLSTLVKSLAGDEEERREVVGLLLELCDFVNVRRRLGRIQGCIVMLVSI 300
           PA+KEMMADVG LSTLVKSLAGDEEERREVVGLLLELCD VNVRRRLGRIQGCIVMLVSI
Sbjct: 241 PANKEMMADVGSLSTLVKSLAGDEEERREVVGLLLELCDLVNVRRRLGRIQGCIVMLVSI 300

Query: 301 LKGDDQIASYDARKLLNVLSGNTQNVLYMAEAGYFKPMVQHLIEGSDMNKILMATGLSRM 360
           LKGDDQIASYDARKLLNVLSGNTQNVLYMAEAGYFKPMVQHLIEGSDMNKILMATGLSRM
Sbjct: 301 LKGDDQIASYDARKLLNVLSGNTQNVLYMAEAGYFKPMVQHLIEGSDMNKILMATGLSRM 360

Query: 361 EHTEQSKASLGEEGVIEPLVQMFRTEKLEAKLSALSALQSLSGLKENVQRLISSGIVVSL 420
           EHTEQSKASLGEEGVIEPLVQMFRTEKLEAKLSALSALQSLSGLKEN+QRLISSGIVVSL
Sbjct: 361 EHTEQSKASLGEEGVIEPLVQMFRTEKLEAKLSALSALQSLSGLKENIQRLISSGIVVSL 420

Query: 421 LQLLFSVTSVLMTLREPAAAILAKISESESILTNYDVALQMLSLLNLSSPIIQNHLLQAL 480
           LQLLFSVTSVLMTLREPAAAILAKISESESILTNYDVALQMLSLLNLSSPIIQNHLLQAL
Sbjct: 421 LQLLFSVTSVLMTLREPAAAILAKISESESILTNYDVALQMLSLLNLSSPIIQNHLLQAL 480

Query: 481 NNIAANPSALEVRKKMVESGAIQLLFPFLMEDNTKVKSGALKLLYTLSKDAPEELEESHI 540
           NNIAANPSALEVRKKMVESGAIQLLFPFLMEDN KVKSGALKLLYTLSKDAPEELEESHI
Sbjct: 481 NNIAANPSALEVRKKMVESGAIQLLFPFLMEDNAKVKSGALKLLYTLSKDAPEELEESHI 540

Query: 541 SLILNIISSTDCKSERVFAVGILSNVPVTQKKITDLLRKANLVPILISIMNSSSANSDIF 600
            +ILNIISSTDCKSERVFAVGILSNVPVTQKKITDLLRKANLVPILISIMNSS++ SD+F
Sbjct: 541 CVILNIISSTDCKSERVFAVGILSNVPVTQKKITDLLRKANLVPILISIMNSSTSKSDVF 600

Query: 601 ASLSSESVAGLLVRFTNPFDRKLQLHSVEQGVIPLLVKLLSSESPVAQSKAAISLAQLSQ 660
           ASLSSESVAGLLVRFTNPFDRKLQLHSVEQGVIPLLVKLLSSES VAQSKAAISLAQLSQ
Sbjct: 601 ASLSSESVAGLLVRFTNPFDRKLQLHSVEQGVIPLLVKLLSSESHVAQSKAAISLAQLSQ 660

Query: 661 NSLSLSKSRTSRWLCVPPSKDSICEVHGRQCLTKRTFCLVKADAIRPMIEILEGKESEVD 720
           NSLSLSKSRTSRWLCVPPSKDSICEVHGRQC  KRTFCLVKADAIRPMIEILEGKESEVD
Sbjct: 661 NSLSLSKSRTSRWLCVPPSKDSICEVHGRQCFIKRTFCLVKADAIRPMIEILEGKESEVD 720

Query: 721 EAVLSALTTLLEDEICDNGSNYVVKMSGVEAILKVLGSGHIDAQQKALWILERIFRIEEH 780
           EAVLSALTTLLEDEICDNGSNYVVKMSGV+AILKVLGSGHID+QQKALWILERIFRIEEH
Sbjct: 721 EAVLSALTTLLEDEICDNGSNYVVKMSGVQAILKVLGSGHIDSQQKALWILERIFRIEEH 780

Query: 781 RVKYGETAWSILVDLSQKGDSSLKSTIAKLLVRLELFQFQHSEYL 825
           RVKYGETAWS+LVDLSQKGDSSLKSTIAKLLVRLELFQFQHSEYL
Sbjct: 781 RVKYGETAWSVLVDLSQKGDSSLKSTIAKLLVRLELFQFQHSEYL 825

BLAST of Cucsat.G4436 vs. ExPASy TrEMBL
Match: A0A1S3BPH2 (U-box domain-containing protein 44-like OS=Cucumis melo OX=3656 GN=LOC103491920 PE=4 SV=1)

HSP 1 Score: 1482 bits (3836), Expect = 0.0
Identity = 791/825 (95.88%), Postives = 807/825 (97.82%), Query Frame = 0

Query: 1   MKEMENRTFSEVVSEIIASTDELASISKNSETENEMFTELALVLEKIPPIFNDLRDYDKI 60
           MKEM+NRTFSEVVSEIIASTDELASISKNSETE EMFTELALVLEKIPPIFNDLRD DKI
Sbjct: 1   MKEMQNRTFSEVVSEIIASTDELASISKNSETEKEMFTELALVLEKIPPIFNDLRDCDKI 60

Query: 61  VDTPTIRKAVESLEKEIKRAKCSIKVHNQKVKHVESIAHDLGRSLGLVLFATVEVSTQFK 120
           +DTPTIRKAVESLEKEIKRAKCSIKVHNQKVKHVESIAHDLGRSLGLVLFATVEVSTQFK
Sbjct: 61  MDTPTIRKAVESLEKEIKRAKCSIKVHNQKVKHVESIAHDLGRSLGLVLFATVEVSTQFK 120

Query: 121 TKIGELHKELMNMKFDENCSPTSTSSRTTEFICDLRVEEIEEERTSVKACDIALHLKYGN 180
           TKIGELHKELM MKFDENCSPTSTSSRTTEFICDLRVEEIEEER S+KACDIALHLKYG 
Sbjct: 121 TKIGELHKELMKMKFDENCSPTSTSSRTTEFICDLRVEEIEEERNSIKACDIALHLKYGK 180

Query: 181 DDEFKLAVAGLKDLIQNKNVDDGWLNEEGIVSILLNRLGSNKSVNRPLIIQVLRYLVWNN 240
           DDEFK AV GLK+LIQ+KNVDDGWLNEEGIVSILLNRLG NK +NRP+IIQVLR LVWNN
Sbjct: 181 DDEFKRAVVGLKELIQSKNVDDGWLNEEGIVSILLNRLGCNKFINRPIIIQVLRCLVWNN 240

Query: 241 PASKEMMADVGPLSTLVKSLAGDEEERREVVGLLLELCDFVNVRRRLGRIQGCIVMLVSI 300
           PA+KEMMADVG LSTLVKSLAGDEEERREVVGLLLELCD VNVRRRLGRIQGCIVMLVSI
Sbjct: 241 PANKEMMADVGSLSTLVKSLAGDEEERREVVGLLLELCDLVNVRRRLGRIQGCIVMLVSI 300

Query: 301 LKGDDQIASYDARKLLNVLSGNTQNVLYMAEAGYFKPMVQHLIEGSDMNKILMATGLSRM 360
           LKGDDQIASYDARKLLNVLSGNTQNVLYMAEAGYFKPMVQHLIEGSDMNKILMATGLSRM
Sbjct: 301 LKGDDQIASYDARKLLNVLSGNTQNVLYMAEAGYFKPMVQHLIEGSDMNKILMATGLSRM 360

Query: 361 EHTEQSKASLGEEGVIEPLVQMFRTEKLEAKLSALSALQSLSGLKENVQRLISSGIVVSL 420
           EHTEQSKASLGEEGVIEPLVQMFRTEKLEAKLSALSALQSLSGLKEN+QRLISSGIVVSL
Sbjct: 361 EHTEQSKASLGEEGVIEPLVQMFRTEKLEAKLSALSALQSLSGLKENIQRLISSGIVVSL 420

Query: 421 LQLLFSVTSVLMTLREPAAAILAKISESESILTNYDVALQMLSLLNLSSPIIQNHLLQAL 480
           LQLLFSVTSVLMTLREPAAAILAKISESESILTNYDVALQMLSLLNLSSPIIQNHLLQAL
Sbjct: 421 LQLLFSVTSVLMTLREPAAAILAKISESESILTNYDVALQMLSLLNLSSPIIQNHLLQAL 480

Query: 481 NNIAANPSALEVRKKMVESGAIQLLFPFLMEDNTKVKSGALKLLYTLSKDAPEELEESHI 540
           NNIAANPSALEVRKKMVESGAIQLLFPFLMEDN KVKSGALKLLYTLSKDAPEELEESHI
Sbjct: 481 NNIAANPSALEVRKKMVESGAIQLLFPFLMEDNAKVKSGALKLLYTLSKDAPEELEESHI 540

Query: 541 SLILNIISSTDCKSERVFAVGILSNVPVTQKKITDLLRKANLVPILISIMNSSSANSDIF 600
            +ILNIISSTDCKSERVFAVGILSNVPVTQKKITDLLRKANLVPILISIMNSS++ SD+F
Sbjct: 541 CVILNIISSTDCKSERVFAVGILSNVPVTQKKITDLLRKANLVPILISIMNSSTSKSDVF 600

Query: 601 ASLSSESVAGLLVRFTNPFDRKLQLHSVEQGVIPLLVKLLSSESPVAQSKAAISLAQLSQ 660
           ASLSSESVAGLLVRFTNPFDRKLQLHSVEQGVIPLLVKLLSSES VAQSKAAISLAQLSQ
Sbjct: 601 ASLSSESVAGLLVRFTNPFDRKLQLHSVEQGVIPLLVKLLSSESHVAQSKAAISLAQLSQ 660

Query: 661 NSLSLSKSRTSRWLCVPPSKDSICEVHGRQCLTKRTFCLVKADAIRPMIEILEGKESEVD 720
           NSLSLSKSRTSRWLCVPPSKDSICEVHGRQC  KRTFCLVKADAIRPMIEILEGKESEVD
Sbjct: 661 NSLSLSKSRTSRWLCVPPSKDSICEVHGRQCFIKRTFCLVKADAIRPMIEILEGKESEVD 720

Query: 721 EAVLSALTTLLEDEICDNGSNYVVKMSGVEAILKVLGSGHIDAQQKALWILERIFRIEEH 780
           EAVLSALTTLLEDEICDNGSNYVVKMSGV+AILKVLGSGHID+QQKALWILERIFRIEEH
Sbjct: 721 EAVLSALTTLLEDEICDNGSNYVVKMSGVQAILKVLGSGHIDSQQKALWILERIFRIEEH 780

Query: 781 RVKYGETAWSILVDLSQKGDSSLKSTIAKLLVRLELFQFQHSEYL 825
           RVKYGETAWS+LVDLSQKGDSSLKSTIAKLLVRLELFQFQHSEYL
Sbjct: 781 RVKYGETAWSVLVDLSQKGDSSLKSTIAKLLVRLELFQFQHSEYL 825

BLAST of Cucsat.G4436 vs. ExPASy TrEMBL
Match: A0A6J1CEH0 (U-box domain-containing protein 44-like OS=Momordica charantia OX=3673 GN=LOC111009991 PE=4 SV=1)

HSP 1 Score: 1311 bits (3393), Expect = 0.0
Identity = 699/820 (85.24%), Postives = 760/820 (92.68%), Query Frame = 0

Query: 1   MKEMENRTFSEVVSEIIASTDELASISKNSETENEMFTELALVLEKIPPIFNDLRDYDKI 60
           + ++ENRTFSEVVSEIIASTDELASISK+SETE EMF+ELALV+EKIPPIFNDLRDYDKI
Sbjct: 10  VNKIENRTFSEVVSEIIASTDELASISKSSETEKEMFSELALVVEKIPPIFNDLRDYDKI 69

Query: 61  VDTPTIRKAVESLEKEIKRAKCSIKVHNQKVKHVESIAHDLGRSLGLVLFATVEVSTQFK 120
           +DTP+IRKAVESLEKEIKRAKC I+VHNQK+KHVE+IAHDLGRSLGLVLFATVEVST+FK
Sbjct: 70  MDTPSIRKAVESLEKEIKRAKCLIEVHNQKMKHVETIAHDLGRSLGLVLFATVEVSTKFK 129

Query: 121 TKIGELHKELMNMKFDENCSPTSTSSRTTEFICDLRVEEIEEERTSVKACDIALHLKYGN 180
            KIGEL+KELM+MKF+ENCSPTSTSS+ TEF CDLRVEEIEEER S   CDIA+ LKYGN
Sbjct: 130 AKIGELYKELMSMKFNENCSPTSTSSQATEFGCDLRVEEIEEERISTNVCDIAVQLKYGN 189

Query: 181 DDEFKLAVAGLKDLIQNKNVDDGWLNEEGIVSILLNRLGSNKSVNRPLIIQVLRYLVWNN 240
           DDEFKLA  GLK+L+Q+KN+D+ WL EEGIVSILLNRLGS+KSV+R +IIQVL+Y+V N 
Sbjct: 190 DDEFKLAAMGLKELMQSKNIDEEWLKEEGIVSILLNRLGSSKSVDRSIIIQVLQYIVLNY 249

Query: 241 PASKEMMADVGPLSTLVKSLAGDEEERREVVGLLLELCDFVNVRRRLGRIQGCIVMLVSI 300
           PA KEMMADVGPLS L KSLAGDE ERRE VGLLL+LC+ VNVRRRLGR+QGC+VMLV+I
Sbjct: 250 PAIKEMMADVGPLSKLAKSLAGDEVERREAVGLLLDLCELVNVRRRLGRVQGCVVMLVAI 309

Query: 301 LKGDDQIASYDARKLLNVLSGNTQNVLYMAEAGYFKPMVQHLIEGSDMNKILMATGLSRM 360
           L GDDQIASYDARKLLNVLSGNTQNVLYMAEAGYF+PMVQHL EGSDMNKILMAT +SRM
Sbjct: 310 LNGDDQIASYDARKLLNVLSGNTQNVLYMAEAGYFRPMVQHLKEGSDMNKILMATAISRM 369

Query: 361 EHTEQSKASLGEEGVIEPLVQMFRTEKLEAKLSALSALQSLSGLKENVQRLISSGIVVSL 420
           E TEQS+ASLGEEG IEPLVQMF  EKLEAKLSALSALQSLS LKENVQRLI SGIV+SL
Sbjct: 370 ELTEQSRASLGEEGAIEPLVQMFWAEKLEAKLSALSALQSLSSLKENVQRLIGSGIVMSL 429

Query: 421 LQLLFSVTSVLMTLREPAAAILAKISESESILTNYDVALQMLSLLNLSSPIIQNHLLQAL 480
           LQLLFSVTSVLMTLREPAAAILAKI+ESESIL N+D+ALQMLSLLNLSSP+IQNHLLQAL
Sbjct: 430 LQLLFSVTSVLMTLREPAAAILAKIAESESILVNHDMALQMLSLLNLSSPVIQNHLLQAL 489

Query: 481 NNIAANPSALEVRKKMVESGAIQLLFPFLMEDNTKVKSGALKLLYTLSKDAPEELEESHI 540
           N IAA+PSA EVRKKMVESGAIQLLFPFLME N K+K+GAL LLY LSKDAPEEL ESHI
Sbjct: 490 NRIAAHPSAAEVRKKMVESGAIQLLFPFLMETNPKIKTGALNLLYNLSKDAPEELGESHI 549

Query: 541 SLILNIISSTDCKSERVFAVGILSNVPVTQKKITDLLRKANLVPILISIMNSSSANSDIF 600
           S+IL II+ST+ +SERVFAVGILSNVPVTQKKITD+LRKANLVP L+SIMNSS  NS+I 
Sbjct: 550 SVILEIITSTNSESERVFAVGILSNVPVTQKKITDMLRKANLVPTLVSIMNSSLTNSNIS 609

Query: 601 ASLSSESVAGLLVRFTNPFDRKLQLHSVEQGVIPLLVKLLSSESPVAQSKAAISLAQLSQ 660
            SL SESVAGLLVRFTNPFDRKLQLHS EQGVIPLLVKLLSS+SPVAQSKAA SLAQLSQ
Sbjct: 610 TSLLSESVAGLLVRFTNPFDRKLQLHSAEQGVIPLLVKLLSSDSPVAQSKAATSLAQLSQ 669

Query: 661 NSLSLSKSRTSRWLCVPPSKDSICEVHGRQCLTKRTFCLVKADAIRPMIEILEGKESEVD 720
           NSLSLSKSRTSRWLCVPPSKDSICEVHGRQC+ K TFCLVKA+A+ PMI+ILEGKE EVD
Sbjct: 670 NSLSLSKSRTSRWLCVPPSKDSICEVHGRQCIIKSTFCLVKANAVPPMIQILEGKEREVD 729

Query: 721 EAVLSALTTLLEDEICDNGSNYVVKMSGVEAILKVLGSGHIDAQQKALWILERIFRIEEH 780
           EAVLSALTTLLEDEICDNGSNY+VKMSGV+AILKVLGS HIDAQQKALWILERIFRIEEH
Sbjct: 730 EAVLSALTTLLEDEICDNGSNYIVKMSGVQAILKVLGSDHIDAQQKALWILERIFRIEEH 789

Query: 781 RVKYGETAWSILVDLSQKGDSSLKSTIAKLLVRLELFQFQ 820
           RV+YGE    +LVDL++KGDSSLKSTIAKLLVRLELFQFQ
Sbjct: 790 RVQYGEPTSFVLVDLAEKGDSSLKSTIAKLLVRLELFQFQ 829

BLAST of Cucsat.G4436 vs. ExPASy TrEMBL
Match: A0A6J1K275 (U-box domain-containing protein 44-like OS=Cucurbita maxima OX=3661 GN=LOC111489427 PE=4 SV=1)

HSP 1 Score: 1301 bits (3368), Expect = 0.0
Identity = 694/822 (84.43%), Postives = 756/822 (91.97%), Query Frame = 0

Query: 1   MKEMENRTFSEVVSEIIASTDELASISKNSETENEMFTELALVLEKIPPIFNDLRDYDKI 60
           +KE+ENRTFSEV+S IIASTDELASISK+SETE EMF ELALVLE+IPPIF+DLR+YDKI
Sbjct: 4   VKEIENRTFSEVISGIIASTDELASISKSSETEKEMFNELALVLERIPPIFSDLREYDKI 63

Query: 61  VDTPTIRKAVESLEKEIKRAKCSIKVHNQKVKHVESIAHDLGRSLGLVLFATVEVSTQFK 120
           +DTP+IRKAV SLEKEI RAKC I V NQK+KHVESIAHDLGRSLGLVLFAT EVSTQFK
Sbjct: 64  MDTPSIRKAVGSLEKEIMRAKCLINVPNQKMKHVESIAHDLGRSLGLVLFATAEVSTQFK 123

Query: 121 TKIGELHKELMNMKFDENCSPTSTS--SRTTEFICDLRVEEIEEERTSVKACDIALHLKY 180
            KIGELHKELMN+KF ENCSPTSTS  SRTTEF+CDLRVEEIEE++ S + CDIA HLKY
Sbjct: 124 AKIGELHKELMNLKFSENCSPTSTSTSSRTTEFVCDLRVEEIEEQQISTRVCDIAHHLKY 183

Query: 181 GNDDEFKLAVAGLKDLIQNKNVDDGWLNEEGIVSILLNRLGSNKSVNRPLIIQVLRYLVW 240
           GNDDEFKLAV GLK+LI NKNVDD WLNEEGIVSILLNR+ S+KS N+ +IIQVLRYLVW
Sbjct: 184 GNDDEFKLAVVGLKELIHNKNVDDEWLNEEGIVSILLNRMDSSKSANQSIIIQVLRYLVW 243

Query: 241 NNPASKEMMADVGPLSTLVKSLAGDEEERREVVGLLLELCDFVNVRRRLGRIQGCIVMLV 300
           N+PASK ++++VG LSTLVKSLAGDEEERRE VGLLLEL D VNVRRRLGR+QGCIVMLV
Sbjct: 244 NSPASKGLISNVGALSTLVKSLAGDEEERREAVGLLLELSDLVNVRRRLGRVQGCIVMLV 303

Query: 301 SILKGDDQIASYDARKLLNVLSGNTQNVLYMAEAGYFKPMVQHLIEGSDMNKILMATGLS 360
           +IL GDDQIAS +ARKLLNVLS NTQNVL+MAEAGYFKPMVQHL EGSDMNKILMATGLS
Sbjct: 304 AILNGDDQIASCNARKLLNVLSVNTQNVLHMAEAGYFKPMVQHLKEGSDMNKILMATGLS 363

Query: 361 RMEHTEQSKASLGEEGVIEPLVQMFRTEKLEAKLSALSALQSLSGLKENVQRLISSGIVV 420
           RMEHTEQSKASLGEEG IEPLVQMFRTEKLEAKLSALSALQSLSGL ENVQRLI SG+V+
Sbjct: 364 RMEHTEQSKASLGEEGAIEPLVQMFRTEKLEAKLSALSALQSLSGLNENVQRLIDSGMVI 423

Query: 421 SLLQLLFSVTSVLMTLREPAAAILAKISESESILTNYDVALQMLSLLNLSSPIIQNHLLQ 480
           SLLQLLFSVTSVLMTLREPAAAILA++SESES L N+++ALQMLSLLNLSSP+IQNHLLQ
Sbjct: 424 SLLQLLFSVTSVLMTLREPAAAILARMSESESFLMNHEMALQMLSLLNLSSPVIQNHLLQ 483

Query: 481 ALNNIAANPSALEVRKKMVESGAIQLLFPFLMEDNTKVKSGALKLLYTLSKDAPEELEES 540
           ALNNI AN SALEVR  MVE+GAIQLLFPF+ME+NTK+KSGALKLLYTLSKDAPEELEES
Sbjct: 484 ALNNIVANSSALEVRINMVENGAIQLLFPFVMENNTKIKSGALKLLYTLSKDAPEELEES 543

Query: 541 HISLILNIISSTDCKSERVFAVGILSNVPVTQKKITDLLRKANLVPILISIMNSSSANSD 600
            IS+IL+IISST CK+ERVFAVGILSNV VTQKK TD+LRKANL+PILISIMNSS ANSD
Sbjct: 544 QISVILSIISSTSCKAERVFAVGILSNVSVTQKKATDMLRKANLLPILISIMNSSLANSD 603

Query: 601 IFASLSSESVAGLLVRFTNPFDRKLQLHSVEQGVIPLLVKLLSSESPVAQSKAAISLAQL 660
           ++ SL SESVAGLLV+FTNPFDRKLQLHS EQGVIPLLVKLLSS+S +AQ KAA SLAQL
Sbjct: 604 VYVSLLSESVAGLLVQFTNPFDRKLQLHSAEQGVIPLLVKLLSSKSAIAQCKAATSLAQL 663

Query: 661 SQNSLSLSKSRTSRWLCVPPSKDSICEVHGRQCLTKRTFCLVKADAIRPMIEILEGKESE 720
           SQNSLSLSKSRT+RWLCVP SKDS+CEVHGRQC  K TFCLVKADAI PMI+ILEGKE E
Sbjct: 664 SQNSLSLSKSRTTRWLCVPYSKDSVCEVHGRQCFIKTTFCLVKADAIPPMIQILEGKERE 723

Query: 721 VDEAVLSALTTLLEDEICDNGSNYVVKMSGVEAILKVLGSGHIDAQQKALWILERIFRIE 780
           VDEAVLSALTTLLEDEICDNGS Y+VKM GV+ ILKVLGSGHID QQKALWILERIFRIE
Sbjct: 724 VDEAVLSALTTLLEDEICDNGSKYIVKMCGVQGILKVLGSGHIDTQQKALWILERIFRIE 783

Query: 781 EHRVKYGETAWSILVDLSQKGDSSLKSTIAKLLVRLELFQFQ 820
           EH V+YGETAWS+LVDLS+KGDSSL+S IAK+L +LELFQ Q
Sbjct: 784 EHGVQYGETAWSVLVDLSKKGDSSLESAIAKVLQQLELFQLQ 825

BLAST of Cucsat.G4436 vs. TAIR 10
Match: AT1G20780.1 (senescence-associated E3 ubiquitin ligase 1 )

HSP 1 Score: 165.2 bits (417), Expect = 2.2e-40
Identity = 190/734 (25.89%), Postives = 345/734 (47.00%), Query Frame = 0

Query: 112 TVEVSTQFKTK-IGELHKELMNMKFDENCSPTSTSSRTTEFICDLRVEEIEEE---RTSV 171
           T+E    F+ + I +  KE  +     +C  TS    +T+    + +    EE   R   
Sbjct: 41  TLENGRTFEREAIEKWFKECRDSGRPPSCPLTSQELTSTDVSASIALRNTIEEWRSRNDA 100

Query: 172 KACDIALH-LKYGN-DDEFKLAVAGLKDLIQNKNVDDGWLNEEGIVSILLNRLGSNKSVN 231
              DIA   L  GN + +   A+  ++ + +    +   +    ++ ++++ L S     
Sbjct: 101 AKLDIARQSLFLGNAETDILQALMHVRQICRTIRSNRHGVRNSQLIHMIIDMLKSTSHRV 160

Query: 232 RPLIIQVLRYLVWNNPASKEMMADVGPLSTLVKSLAGDEEERRE-VVGLLLELCDFVNVR 291
           R   +Q L+ +V  +  SK ++A+   + TLVK L+ +  + RE  V LL EL     + 
Sbjct: 161 RYKALQTLQVVVEGDDESKAIVAEGDTVRTLVKFLSHEPSKGREAAVSLLFELSKSEALC 220

Query: 292 RRLGRIQGCIVMLVSILKGDDQIASY--DARKLLNVLSGNTQNVLYMAEAGYFKPMVQHL 351
            ++G I G +++LV +   + +  S    A + L  +  + + V  MA  G  +P++  L
Sbjct: 221 EKIGSIHGALILLVGLTSSNSENVSIVEKADRTLENMERSEEIVRQMASYGRLQPLLGKL 280

Query: 352 IEGSDMNKILMATGLSRMEHTEQSKASLGEEGVIEPLVQMFRTEKLEAKLSALSALQSLS 411
           +EGS   K+ MA+ L  +      K  L  + V   LV + R+  +  + +AL AL  +S
Sbjct: 281 LEGSPETKLSMASFLGELPLNNDVKV-LVAQTVGSSLVDLMRSGDMPQREAALKALNKIS 340

Query: 412 GLKENVQRLISSGIVVSLLQLLFSV--TSVLMTLREPAAAILAKISE-----SESILTNY 471
             + + + LIS GI+  L++ LF V   ++ + L+E +A ILA I        ++ L + 
Sbjct: 341 SFEGSAKVLISKGILPPLIKDLFYVGPNNLPIRLKEVSATILANIVNIGYDFDKATLVSE 400

Query: 472 DVALQMLSLLNLSSPIIQNHLLQALNNIAANPSAL-EVRKKMVESGAIQLLFPFL-MEDN 531
           +    +L L++ + P IQ  LL+ L  + + P  + +V   +  SGAI  L  F+ + +N
Sbjct: 401 NRVENLLHLISNTGPAIQCKLLEVLVGLTSCPKTVPKVVYAIKTSGAIISLVQFIEVREN 460

Query: 532 TKVKSGALKLLYTLSKDAPEELEESHI-------SLILNIISSTDCKSERVFAVGILSNV 591
             ++  ++KLL+ LS    EEL ++         SL+  I   T    E+  A G+L+ +
Sbjct: 461 DDLRLASIKLLHNLSPFMSEELAKALCGTAGQLGSLVAIISEKTPITEEQAAAAGLLAEL 520

Query: 592 PVTQKKITDLLRKANLVPILISI---MNSSSANSDIFASLSSESVAGLLVRFTNPFDRKL 651
           P     +T  + +      +IS    +         F +   E +  +L R T  F+++ 
Sbjct: 521 PDRDLGLTQEMLEVGAFEKIISKVFGIRQGDIKGMRFVNPFLEGLVRILARITFVFNKEA 580

Query: 652 QLHSV--EQGVIPLLVKLLSSE-SPVAQSKAAISLAQLSQNSLSLSKSRT--------SR 711
           +  +   E  V  L + LL S      Q  +A++L  LS  S+ L++           S 
Sbjct: 581 RAINFCREHDVASLFLHLLQSNGQDNIQMVSAMALENLSLESIKLTRMPDPPPVNYCGSI 640

Query: 712 WLCV--PPSKDSICEVHGRQCLTKRTFCLVKADAIRPMIEILEGKESEVDEAVLSALTTL 771
           + CV  P   + +C++H   C  + TFCLV+  A+  ++ +L+ +  +V EA L+AL++L
Sbjct: 641 FSCVRKPHVVNGLCKIHQGICSLRETFCLVEGGAVEKLVALLDHENVKVVEAALAALSSL 700

Query: 772 LEDEI-CDNGSNYVVKMSGVEAILKVLGSGHID-AQQKALWILERIFRIEE--HRVKYGE 801
           LED +  + G   + +  G+  IL VL     +   ++A+W++ERI RIE+    V   +
Sbjct: 701 LEDGLDVEKGVKILDEADGIRHILNVLRENRTERLTRRAVWMVERILRIEDIAREVAEEQ 760

BLAST of Cucsat.G4436 vs. TAIR 10
Match: AT1G76390.1 (ARM repeat superfamily protein )

HSP 1 Score: 163.7 bits (413), Expect = 6.3e-40
Identity = 192/730 (26.30%), Postives = 335/730 (45.89%), Query Frame = 0

Query: 138 NCSPTSTSSRTTEFICDLRVEEIEEE---RTSVKACDIALHLKY-GN-DDEFKLAVAGLK 197
           +C  TS     T+    + +    EE   R      DIA    Y GN +    LA+  ++
Sbjct: 70  SCPITSKELSITDLSPSIALRNTIEEWRARNDALKLDIARQSLYLGNAETNILLALKNVR 129

Query: 198 DLIQNKNVDDGWLNEEGIVSILLNRLGSNKSVNRPLIIQVLRYLVWNNPASKEMMADVGP 257
           ++ +N       +    +V ++ + L S+    R   +Q L+ +V  +  SK ++A+   
Sbjct: 130 EICRNIRKIRQRVCNPQLVRLITDMLKSSSHEVRCKALQTLQVVVEGDEESKAIVAEGDT 189

Query: 258 LSTLVKSLAGDEEERRE-VVGLLLELCDFVNVRRRLGRIQGCIVMLVSIL--KGDDQIAS 317
           + T+VK L+ +  + RE  V +L EL     +  ++G I G I++LV +   K ++    
Sbjct: 190 VRTIVKFLSQEPSKGREAAVSVLFELSKSEALCEKIGSIHGAIILLVGLTSSKSENVSTV 249

Query: 318 YDARKLLNVLSGNTQNVLYMAEAGYFKPMVQHLIEGSDMNKILMATGLSRMEHTEQSKAS 377
             A K L  L  + +NV  MA  G  +P++  L+EGS   K+ MA  L  +      K  
Sbjct: 250 EKADKTLTNLERSEENVRQMAINGRLQPLLAKLLEGSPETKVSMAFYLGVLALNNDVKVI 309

Query: 378 LGEEGVIEPLVQMFRTEKLEAKLSALSALQSLSGLKENVQRLISSGIVVSLLQLLFSV-- 437
           + +  V   L+ + RT  +  + +AL AL ++S  + + + LI++GI+  L++ LF V  
Sbjct: 310 VAQT-VGSSLIDLMRTRDMSQREAALGALNNISSFEGSAKLLINTGILPPLIKDLFYVGP 369

Query: 438 TSVLMTLREPAAAILAKI------------SESESILTNYDVALQMLSLLNLSSPIIQNH 497
             + + L+E +A ILA I                  L + ++   +L L + + P IQ  
Sbjct: 370 NQLPIRLKEVSATILANIVNIGYDFDKVPVGPHHQTLVSEEIVENLLQLTSNTGPEIQGK 429

Query: 498 LLQALNNIAANP-SALEVRKKMVESGAIQLLFPFL-MEDNTKVKSGALKLLYTLSKDAPE 557
           LL  L  + + P S + V   +  S AI  L  F+ + +N  ++  ++KLL+ +S    E
Sbjct: 430 LLAVLVGLTSCPNSVINVVSAIRNSAAIISLVQFVEIHENDDLRLASIKLLHNISPHMSE 489

Query: 558 ELEES------HISLILNIIS--STDCKSERVFAVGILSNVPVTQKKIT-DLLRKANLVP 617
           EL  +       +  +++IIS  +     E+  A G+L+ +P     +T  LLR+     
Sbjct: 490 ELANALRSTVGQLGSLVSIISENTPTITEEQAAAAGLLAELPERDLVLTMRLLREGAFEK 549

Query: 618 ILISI--MNSSSANSDIFASLSSESVAGLLVRFTNPFDRKLQ--LHSVEQGVIPLLVKLL 677
           I+  I  +         F     E +  +L R T    ++    L   E+ +  L + LL
Sbjct: 550 IISKIVGIRQGEIRGIRFERTFLEGLVSILARITFALTKETDATLFCCEKNLPSLFLDLL 609

Query: 678 SSES-PVAQSKAAISLAQLSQNSLSLSKSR--------TSRWLCV--PPSKDSICEVHGR 737
            S S    Q  +A +L  LS  S +L+K           S + C+  PP    IC++H  
Sbjct: 610 QSNSQDNIQRASATALENLSLESKNLTKIPELPPPTYCVSIFSCLSKPPVVLGICKIHQG 669

Query: 738 QCLTKRTFCLVKADAIRPMIEILEGKESEVDEAVLSALTTLLEDEI-CDNGSNYVVKMSG 797
            C  + +FCLV+  A+  ++++L+ +  +V    L+AL+TLLED +    G   + +  G
Sbjct: 670 ICSVRESFCLVEGQAVDKLVDLLDHENDKVVGPALAALSTLLEDGLDVVQGVRLIDEADG 729

Query: 798 VEAILKVLGSGHID-AQQKALWILERIFRIEEHRVKYGE--TAWSILVDLSQKGDSSLKS 816
           +  IL VL     +  + +A+W++ERI RIEE   + GE     + LVD  Q  D   + 
Sbjct: 730 ITPILNVLLENRTENLRIRAVWMVERILRIEEIAREVGEEQNVTAALVDAFQNADFRTRQ 789

BLAST of Cucsat.G4436 vs. TAIR 10
Match: AT1G76390.2 (ARM repeat superfamily protein )

HSP 1 Score: 163.7 bits (413), Expect = 6.3e-40
Identity = 192/730 (26.30%), Postives = 335/730 (45.89%), Query Frame = 0

Query: 138 NCSPTSTSSRTTEFICDLRVEEIEEE---RTSVKACDIALHLKY-GN-DDEFKLAVAGLK 197
           +C  TS     T+    + +    EE   R      DIA    Y GN +    LA+  ++
Sbjct: 70  SCPITSKELSITDLSPSIALRNTIEEWRARNDALKLDIARQSLYLGNAETNILLALKNVR 129

Query: 198 DLIQNKNVDDGWLNEEGIVSILLNRLGSNKSVNRPLIIQVLRYLVWNNPASKEMMADVGP 257
           ++ +N       +    +V ++ + L S+    R   +Q L+ +V  +  SK ++A+   
Sbjct: 130 EICRNIRKIRQRVCNPQLVRLITDMLKSSSHEVRCKALQTLQVVVEGDEESKAIVAEGDT 189

Query: 258 LSTLVKSLAGDEEERRE-VVGLLLELCDFVNVRRRLGRIQGCIVMLVSIL--KGDDQIAS 317
           + T+VK L+ +  + RE  V +L EL     +  ++G I G I++LV +   K ++    
Sbjct: 190 VRTIVKFLSQEPSKGREAAVSVLFELSKSEALCEKIGSIHGAIILLVGLTSSKSENVSTV 249

Query: 318 YDARKLLNVLSGNTQNVLYMAEAGYFKPMVQHLIEGSDMNKILMATGLSRMEHTEQSKAS 377
             A K L  L  + +NV  MA  G  +P++  L+EGS   K+ MA  L  +      K  
Sbjct: 250 EKADKTLTNLERSEENVRQMAINGRLQPLLAKLLEGSPETKVSMAFYLGVLALNNDVKVI 309

Query: 378 LGEEGVIEPLVQMFRTEKLEAKLSALSALQSLSGLKENVQRLISSGIVVSLLQLLFSV-- 437
           + +  V   L+ + RT  +  + +AL AL ++S  + + + LI++GI+  L++ LF V  
Sbjct: 310 VAQT-VGSSLIDLMRTRDMSQREAALGALNNISSFEGSAKLLINTGILPPLIKDLFYVGP 369

Query: 438 TSVLMTLREPAAAILAKI------------SESESILTNYDVALQMLSLLNLSSPIIQNH 497
             + + L+E +A ILA I                  L + ++   +L L + + P IQ  
Sbjct: 370 NQLPIRLKEVSATILANIVNIGYDFDKVPVGPHHQTLVSEEIVENLLQLTSNTGPEIQGK 429

Query: 498 LLQALNNIAANP-SALEVRKKMVESGAIQLLFPFL-MEDNTKVKSGALKLLYTLSKDAPE 557
           LL  L  + + P S + V   +  S AI  L  F+ + +N  ++  ++KLL+ +S    E
Sbjct: 430 LLAVLVGLTSCPNSVINVVSAIRNSAAIISLVQFVEIHENDDLRLASIKLLHNISPHMSE 489

Query: 558 ELEES------HISLILNIIS--STDCKSERVFAVGILSNVPVTQKKIT-DLLRKANLVP 617
           EL  +       +  +++IIS  +     E+  A G+L+ +P     +T  LLR+     
Sbjct: 490 ELANALRSTVGQLGSLVSIISENTPTITEEQAAAAGLLAELPERDLVLTMRLLREGAFEK 549

Query: 618 ILISI--MNSSSANSDIFASLSSESVAGLLVRFTNPFDRKLQ--LHSVEQGVIPLLVKLL 677
           I+  I  +         F     E +  +L R T    ++    L   E+ +  L + LL
Sbjct: 550 IISKIVGIRQGEIRGIRFERTFLEGLVSILARITFALTKETDATLFCCEKNLPSLFLDLL 609

Query: 678 SSES-PVAQSKAAISLAQLSQNSLSLSKSR--------TSRWLCV--PPSKDSICEVHGR 737
            S S    Q  +A +L  LS  S +L+K           S + C+  PP    IC++H  
Sbjct: 610 QSNSQDNIQRASATALENLSLESKNLTKIPELPPPTYCVSIFSCLSKPPVVLGICKIHQG 669

Query: 738 QCLTKRTFCLVKADAIRPMIEILEGKESEVDEAVLSALTTLLEDEI-CDNGSNYVVKMSG 797
            C  + +FCLV+  A+  ++++L+ +  +V    L+AL+TLLED +    G   + +  G
Sbjct: 670 ICSVRESFCLVEGQAVDKLVDLLDHENDKVVGPALAALSTLLEDGLDVVQGVRLIDEADG 729

Query: 798 VEAILKVLGSGHID-AQQKALWILERIFRIEEHRVKYGE--TAWSILVDLSQKGDSSLKS 816
           +  IL VL     +  + +A+W++ERI RIEE   + GE     + LVD  Q  D   + 
Sbjct: 730 ITPILNVLLENRTENLRIRAVWMVERILRIEEIAREVGEEQNVTAALVDAFQNADFRTRQ 789

BLAST of Cucsat.G4436 vs. TAIR 10
Match: AT1G68940.1 (Armadillo/beta-catenin-like repeat family protein )

HSP 1 Score: 150.2 bits (378), Expect = 7.2e-36
Identity = 184/747 (24.63%), Postives = 339/747 (45.38%), Query Frame = 0

Query: 136  DENCSPTSTSSRTTEFICDLRVE------EIEEERTSVKACDIALHLKYGNDDEFKLAVA 195
            DE   P +    TTE   ++ ++      ++  E   +K    AL L  G++     A+ 
Sbjct: 287  DEINCPVTGQKLTTELSANVVLKTIIQEWKVRNEAARIKVAHAALSLG-GSESMVIDALR 346

Query: 196  GLKDLIQNKNVDDGWLNEEGIVSILLNRLGSNKSVNRPLIIQVLRYLV-WNNPASKEMMA 255
             L+   + K  +   + E GI+ +L   L       R  +++ LR L        KEM+ 
Sbjct: 347  DLQMTCEGKEYNKVQVREAGIIQLLDRYLTYRSKDVRFELLKFLRTLADEETDDGKEMIV 406

Query: 256  DVGPLSTLVKSLAGDEEE-RREVVGLLLELCDFVNVRRRLGRIQGCIVMLVSIL--KGDD 315
                +S ++K L    +  R     LLLEL    +   ++G  +G I+MLV+    +  D
Sbjct: 407  KTITMSCVIKLLGSSHQPVRHAAQALLLELSKSQHACEKIGTARGAILMLVTAKYNRELD 466

Query: 316  QIASYDARKLLNVLSGNTQNVLYMAEAGYFKPMVQHLIEGSDMNKILMATGLSRMEHTEQ 375
              AS  + ++L  L    +N+  MAE+G  +P++ HL EGS+  ++ MA  L  ++   +
Sbjct: 467  SFASETSDQILRNLEKCPENIKQMAESGLLEPLLGHLAEGSEETQVAMAAYLVEIDIGHE 526

Query: 376  SKASLGEEGVIEPLVQMFRTEKLEAKLSALSALQSLSGLKENVQRLISSGIVVSLLQLLF 435
             K  + E+     L+ + ++E ++A+ +A  AL  +S    N + L+  GI+  +++ +F
Sbjct: 527  KKTYVAEK-ACPALIGLVQSENIDARRAAFKALAHISLYHPNNKILVEVGIIKIMVEEMF 586

Query: 436  S--VTSVLMTLREPAAAILAKISESESILTNYDV------------ALQMLSLLNLSSPI 495
            +  V S LM  R  AA ILA I ES      ++V               ++ +L  SSP 
Sbjct: 587  TKRVFSDLMNSRNEAATILANILESGLEHETFEVNTHGHTLGSDYFVYNIIHMLKNSSPD 646

Query: 496  IQN-HLLQALNNIAANPSALEVRKKMV-ESGAIQLLFPFLMEDNTKVKSGALKLLYTLSK 555
              N  L++ L +++ +P A+     ++ E+ A   +   +   + ++  GALKLL  L+ 
Sbjct: 647  DLNIDLIRILLSLSKSPRAMATIVSVIKETDASFAMIELINNPHDELGVGALKLLIALTP 706

Query: 556  DAPEELEE-------SHISLILNIISSTDCKSERVFAVGILSNVPVTQKKITDLLRKANL 615
                 L E          +LI   + +     +   +  +L+ +P     +   L   ++
Sbjct: 707  YIGHTLSERLCKTRGQPENLIQCPVEANQITEKHAVSAKLLAKLPHQNLTLNLALVNESI 766

Query: 616  VPIL---ISIMNSSSANSDIFASLSSESVAGLLVRFTNPFDRKLQL-----HSVEQGVIP 675
            V  +   I ++  S A +  +A+   E + G+LVRFT        +     H +    + 
Sbjct: 767  VSEILHAIHLIQRSGARTSRYATDFLEGLVGILVRFTTTLYEPQMMYLARNHDLTSVFVD 826

Query: 676  LLVKLLSSESPVAQSKAAISLAQLSQNSLSLSK---SRTSRW---LCVPPS--------- 735
            LL+K  S E    Q  +A  L  LS  +++LS+    R++++   L +P S         
Sbjct: 827  LLMKTSSDE---VQRLSATGLENLSSTTMTLSRPPQPRSTKFMGSLSMPRSFSLRSSKKK 886

Query: 736  KDSICEVHGRQCLTKRTFCLVKADAIRPMIEILEGKESEVDEAVLSALTTLLEDEI-CDN 795
            +  IC +H   C  K TFCLV+A+AI  ++  L+  + EV E+ L+A+ TLL+D++  + 
Sbjct: 887  QIEICAIHRGVCSAKNTFCLVEANAITKLLACLQSDKVEVVESALAAICTLLDDKVEVEK 946

Query: 796  GSNYVVKMSGVEAILKVLGSGHIDA-QQKALWILERIF---------RIEEHRVKYGETA 816
              + + +M+ V+ IL  +     ++  QKA W++++            I + R+  G   
Sbjct: 947  SLSMLSEMNAVQLILNAVKEHKKESLLQKAFWMIDKFIIRGGDKYASEISQDRMLSG--- 1006

BLAST of Cucsat.G4436 vs. TAIR 10
Match: AT1G68940.2 (Armadillo/beta-catenin-like repeat family protein )

HSP 1 Score: 146.4 bits (368), Expect = 1.0e-34
Identity = 174/696 (25.00%), Postives = 321/696 (46.12%), Query Frame = 0

Query: 136 DENCSPTSTSSRTTEFICDLRVE------EIEEERTSVKACDIALHLKYGNDDEFKLAVA 195
           DE   P +    TTE   ++ ++      ++  E   +K    AL L  G++     A+ 
Sbjct: 287 DEINCPVTGQKLTTELSANVVLKTIIQEWKVRNEAARIKVAHAALSLG-GSESMVIDALR 346

Query: 196 GLKDLIQNKNVDDGWLNEEGIVSILLNRLGSNKSVNRPLIIQVLRYLV-WNNPASKEMMA 255
            L+   + K  +   + E GI+ +L   L       R  +++ LR L        KEM+ 
Sbjct: 347 DLQMTCEGKEYNKVQVREAGIIQLLDRYLTYRSKDVRFELLKFLRTLADEETDDGKEMIV 406

Query: 256 DVGPLSTLVKSLAGDEEE-RREVVGLLLELCDFVNVRRRLGRIQGCIVMLVSIL--KGDD 315
               +S ++K L    +  R     LLLEL    +   ++G  +G I+MLV+    +  D
Sbjct: 407 KTITMSCVIKLLGSSHQPVRHAAQALLLELSKSQHACEKIGTARGAILMLVTAKYNRELD 466

Query: 316 QIASYDARKLLNVLSGNTQNVLYMAEAGYFKPMVQHLIEGSDMNKILMATGLSRMEHTEQ 375
             AS  + ++L  L    +N+  MAE+G  +P++ HL EGS+  ++ MA  L  ++   +
Sbjct: 467 SFASETSDQILRNLEKCPENIKQMAESGLLEPLLGHLAEGSEETQVAMAAYLVEIDIGHE 526

Query: 376 SKASLGEEGVIEPLVQMFRTEKLEAKLSALSALQSLSGLKENVQRLISSGIVVSLLQLLF 435
            K  + E+     L+ + ++E ++A+ +A  AL  +S    N + L+  GI+  +++ +F
Sbjct: 527 KKTYVAEK-ACPALIGLVQSENIDARRAAFKALAHISLYHPNNKILVEVGIIKIMVEEMF 586

Query: 436 S--VTSVLMTLREPAAAILAKISESESILTNYDV------------ALQMLSLLNLSSPI 495
           +  V S LM  R  AA ILA I ES      ++V               ++ +L  SSP 
Sbjct: 587 TKRVFSDLMNSRNEAATILANILESGLEHETFEVNTHGHTLGSDYFVYNIIHMLKNSSPD 646

Query: 496 IQN-HLLQALNNIAANPSALEVRKKMV-ESGAIQLLFPFLMEDNTKVKSGALKLLYTLSK 555
             N  L++ L +++ +P A+     ++ E+ A   +   +   + ++  GALKLL  L+ 
Sbjct: 647 DLNIDLIRILLSLSKSPRAMATIVSVIKETDASFAMIELINNPHDELGVGALKLLIALTP 706

Query: 556 DAPEELEE-------SHISLILNIISSTDCKSERVFAVGILSNVPVTQKKITDLLRKANL 615
                L E          +LI   + +     +   +  +L+ +P     +   L   ++
Sbjct: 707 YIGHTLSERLCKTRGQPENLIQCPVEANQITEKHAVSAKLLAKLPHQNLTLNLALVNESI 766

Query: 616 VPIL---ISIMNSSSANSDIFASLSSESVAGLLVRFTNPFDRKLQL-----HSVEQGVIP 675
           V  +   I ++  S A +  +A+   E + G+LVRFT        +     H +    + 
Sbjct: 767 VSEILHAIHLIQRSGARTSRYATDFLEGLVGILVRFTTTLYEPQMMYLARNHDLTSVFVD 826

Query: 676 LLVKLLSSESPVAQSKAAISLAQLSQNSLSLSK---SRTSRW---LCVPPS--------- 735
           LL+K  S E    Q  +A  L  LS  +++LS+    R++++   L +P S         
Sbjct: 827 LLMKTSSDE---VQRLSATGLENLSSTTMTLSRPPQPRSTKFMGSLSMPRSFSLRSSKKK 886

Query: 736 KDSICEVHGRQCLTKRTFCLVKADAIRPMIEILEGKESEVDEAVLSALTTLLEDEI-CDN 774
           +  IC +H   C  K TFCLV+A+AI  ++  L+  + EV E+ L+A+ TLL+D++  + 
Sbjct: 887 QIEICAIHRGVCSAKNTFCLVEANAITKLLACLQSDKVEVVESALAAICTLLDDKVEVEK 946