Cucsat.G20977 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G20977
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	cytochrome P450 81E8-like
Location	ctg907: 475047 .. 477394 (+)
RNA-Seq Expression	Cucsat.G20977
Synteny	Cucsat.G20977

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAAATTAACTGCAAATGAAACAAAATTCAGTTTAAGAATGGGGAATTATCTGTCTCACTTCACTGTCTACATCAACGATTGGTTATTCAAAGTACACTCAGTGGATTTGAGATAAATTTTCCCTTTCCATTTTGCCCATAAAATGACCATTCTGCCCTCACTGGGTTAATTGATTTCTTTTCTCTTTCAGGATATTTAAGGAAACTTGAAAGGAATTTGTGTATAAGATTTTTTCTGGCTCTTCTTCGTGACTTGAGGTTTTATGGGTTTTTGGTTTTCACCCAACCAAGAAAGCTAATGTTTCTTCTTCGGATGGAAGATAGTTCATGGATGGTAACGTTTTTCTTCTTTAATTCATTTAACATTTTAGTTTTGAAATTTTCAGTATTGATACTGTTTTTGAGATTTTGTTGTAGAGGACATTCAGTTTACAATGAATAATGTAAATATTATATTCTTTGTTCATATATCTAAGTTTGTCTTCCAGGATTCTCTTTCACTAAAGATTCATTTTTTTCATTAATTATTTAGTTTCTATGAAATCTGAATCAATTACAAGGAATCACAGAAGCGATTACTCACGAGGTCAGTTTATCTAATTTTAATTTACGGAGATCCATATAACTTTTTTTTTCTTTCAATTATGGATTTCAATTTTGTTCATTTTCTTTCTACATTTTTTTTTGTCAATAATTTACTCTTTTACAACTTGTATATAATTGGCCGTATATTTCACCATACAAATTATATACAATTATATATCCATTATTTCTTTCTTCTTGTTTTAAAATAATATAACTTTCTATTAATTTTTTTATAGTTTAAACAAAATGTTAGATCACATAAATTTAGTTTTTTTAATTTCCATCAAACCCATTTTTTAGTTTAAACTTAGAGGTAAAAACAAATTTAATAGAATGTATAACACTCATCTATATTTAAGTATTATCTAAGTTTTTGAGTGATGATAAATTTAATATAACATCCAGTGTAACTAAATTGCCGAATTGACTTGCAGGTAAGAAAGCAACTTGAAAAATTTAAACGGAACTCAATGATACTTCGACCAACCCAACTAGCATGGTTATAATTTACAAATGATTCTCGCAGGTAAATTTCATGGTGATCCCATTCCTACATCTTCTGATTTTATTCTTACTTCAAATCTTCACAAATCACTTCCTTCATAGAATCCGAAATCTCCCTCCCAGCCCATTTCTTTCTCTACCCATTATAGGCCAACTATACCTCCTTAAAACACCCCTCCACCGGACCTTATCGGAGATCTCCCGCCGTCATGGCCCAATCGTCTTCTTCCAATTCGGTTTCCGTTCAGTCCTGGTCGTTTCATCTCCGTCGGCCGCCGAGGACTGCCTATCCAAAAACGACGTCATCTTCGCCAACCGACCTCGTCTCGTTAGCGGCAAATACCTCGGGTACAATTACACAAGCCTCCTGTGGGCACCGTACGGCGAGCATTGGCGAAACCTCCGTCGAATCTCGTCGTTAGAAATCCTTTCCAGCCACCGCCTCCAAACGCTTTCAAGTATTCGCGTGGATGAAGTCCGTACGCTGATCCGGAGACTGTACAACGCTGAAAACGACGTCGTAGACATGAGAACAGAATTTTTCGAAGTGATGTTCAATATCACGATGAGAATGATCGCCGGAAAACGATATTACGGGGAAGATGTGGCAGCGTACTCGGAGGAGGCGGGGAGATTTCGAGAGATACAAGAGGAGACGTTTCGATTAAGCGGCAAGACTAATTTGGGGGACTTTTTAAGGGTTGTGAAATGGGTTGGATTATCTAAAGGGATTGAAAATAGATTGAGGGAGTTACAGATTAAGAGAGATGATTGGATGCAGAGTTTGATTGAAGAACATCGGAAGAAAATGAATAATGCTTATAGTTCTTCTTCTTCTATTCAAACTGATGCTAAAAAGACTATGATTGAGGTTCTCCTCTCATTGCAACAAAAGGAACCTCAGTATTATAAGGACGAGTATATCAGAGGCCTCATGCTCGTGAGTTAACTTGATTTCTCTCTTAGTTATTCAAAAGACATAACCAAATAGATTTTGAAACACTATTTTAAACACATATGAAAATATATGTAATCCGACTCTATTTTTCCTTAAATCAACCAAATCGTCCCAAATGAAATTGGATTGTTGTAGGTGTTGCTATTAGCTGGAACGGAAGGTTCCATCAATACAATGGAGTGGCTTCTATCACTATTACTCAACCACCCACACTCTCTTCAACGAGCCCAAATGGAAATCGACGACGTAGTTGGAAGAACTAATCGGCTCCTGGAGGAATCAGATCTAACCCACCTTCCTTACCTCCGTAGCCTGATCCACGAAACTCTTCGAATGTACCCGCCAGGCCCATTACTGATTCCACACGAATCGTCAGAGGATTGCCACGTTGGAGGGTTCCATGTCCCAGCTGGCACAATGTTATTCGTCAACGTATGGGCCATTCAAAACGACCCCACCGTATGGGTGGAGCCGAGGAAATTTAACCCAGATAGATTCGGAGGGGATGGAGAGGGGTTCAAATGGATGCCATTTGGGGCTGGGCGGAGGAGGTGTCCCGGCGAGGGCTTGGGGCTTCGCGTCATTGGATTGGTTGTAGGGTCGCTGATTCAATGCTTTGAATGGGAGTCAATGGACGGAGAATGTATTGATATGAGCGAAGGTGGTGGATTGACCTTGCCGAAAGCTCTCCCCCTTCGCACCCTTTGCCGGCCACGTTCAAATGCCACACATTTGCTTTCCCAAATCTAATTCAAAATGCATGGTCTTGTTTATGTTGTTCAACAACAATTAATTCTGTACATAAATTTTACATATTTTAGTAGTAGTTGAAAACACCTACGTTATTTCCAATTTGTTGACGTCCTATTTTTCTTTTTTCTTTTGCCTCTTTGTTTACGTGATGATTGGTTGATGTCCTATATTTTAGTAGAAGCAAAAG

Coding sequence (CDS)

ATGGTGATCCCATTCCTACATCTTCTGATTTTATTCTTACTTCAAATCTTCACAAATCACTTCCTTCATAGAATCCGAAATCTCCCTCCCAGCCCATTTCTTTCTCTACCCATTATAGGCCAACTATACCTCCTTAAAACACCCCTCCACCGGACCTTATCGGAGATCTCCCGCCGTCATGGCCCAATCGTCTTCTTCCAATTCGGTTTCCGTTCAGTCCTGGTCGTTTCATCTCCGTCGGCCGCCGAGGACTGCCTATCCAAAAACGACGTCATCTTCGCCAACCGACCTCGTCTCGTTAGCGGCAAATACCTCGGGTACAATTACACAAGCCTCCTGTGGGCACCGTACGGCGAGCATTGGCGAAACCTCCGTCGAATCTCGTCGTTAGAAATCCTTTCCAGCCACCGCCTCCAAACGCTTTCAAGTATTCGCGTGGATGAAGTCCGTACGCTGATCCGGAGACTGTACAACGCTGAAAACGACGTCGTAGACATGAGAACAGAATTTTTCGAAGTGATGTTCAATATCACGATGAGAATGATCGCCGGAAAACGATATTACGGGGAAGATGTGGCAGCGTACTCGGAGGAGGCGGGGAGATTTCGAGAGATACAAGAGGAGACGTTTCGATTAAGCGGCAAGACTAATTTGGGGGACTTTTTAAGGGTTGTGAAATGGGTTGGATTATCTAAAGGGATTGAAAATAGATTGAGGGAGTTACAGATTAAGAGAGATGATTGGATGCAGAGTTTGATTGAAGAACATCGGAAGAAAATGAATAATGCTTATAGTTCTTCTTCTTCTATTCAAACTGATGCTAAAAAGACTATGATTGAGGTTCTCCTCTCATTGCAACAAAAGGAACCTCAGTATTATAAGGACGAGTATATCAGAGGCCTCATGCTCGTGAGTTAA

Protein sequence

MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRHGPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMRMIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRELQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRGLMLVS

Homology

[Close]

BLAST of Cucsat.G20977 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 3.4e-134
Identity = 248/511 (48.53%), Postives = 351/511 (68.69%), Query Frame = 0


Query: 1   MVIPFLHLLILFL-LQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRR 60
           ++  FL +++L + L++F      R RNLPPSP L+LP+IG L+L+   LHR+L ++S++
Sbjct: 6   LLYTFLAVVLLSISLKLFP--VSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKK 65

Query: 61  HGPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGE 120
           +G +   Q G R VLVVSSP+AAE+C +KND++FANRP  + GKY+GYNYT+++ +PYGE
Sbjct: 66  YGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGE 125

Query: 121 HWRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAEND---VVDMRTEFFEVMFN 180
           HWRNLRR++++EI S+  L    SIR DEV+ L+  LY +       V+M+++  E+ FN
Sbjct: 126 HWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFN 185

Query: 181 ITMRMIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIEN 240
           +TMRM+AGKRY+G+DV   S+EA  FR +  E F  +G +N GDF+  ++W+   K  E 
Sbjct: 186 VTMRMVAGKRYFGQDVD--SDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDF-KNYEK 245

Query: 241 RLRELQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDE 300
           ++ ++  + D ++Q LI E R   NN              TMI+ LLSLQ+ +P+YY D+
Sbjct: 246 KVSKISQEMDAFLQRLIHESRINKNNV-------------TMIDHLLSLQESQPEYYTDQ 305

Query: 301 YIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTH 360
            I+G+++VLLLAGT+ S  T+EW +SLLLNHP +L++A+ EI+  VG +NRL+EE DL  
Sbjct: 306 IIKGIIMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVG-SNRLIEEQDLPK 365

Query: 361 LPYLRSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEP 420
           L YL ++I ET R+ P  P+L+PHESS+DC V G+ VP GT+L VN WAI  DP  W EP
Sbjct: 366 LTYLHNIISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEP 425

Query: 421 RKFNPDRFGG-DGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECID 480
             F P+R GG + E  K MPFG GRR CPG GL  RV+GL +G+LIQCFEW+ +    ID
Sbjct: 426 TLFKPERHGGVELEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKID 485

Query: 481 MSEGGGLTLPKALPLRTLCRPRSNATHLLSQ 507
           M+EG GLT+PKA PL  LC+PR+    ++S+
Sbjct: 486 MAEGSGLTMPKAQPLEALCKPRNILHKVVSE 497

[Close]

BLAST of Cucsat.G20977 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 440.3 bits (1131), Expect = 3.0e-122
Identity = 224/495 (45.25%), Postives = 329/495 (66.46%), Query Frame = 0


Query: 7   HLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRHGPIVFF 66
           +L + F+  I       + +NLPP P  SLPIIG L+ LK PLHRT   +S ++G +   
Sbjct: 13  YLALFFIFNIVIR--ARKFKNLPPGP-PSLPIIGNLHHLKRPLHRTFKGLSEKYGHVFSL 72

Query: 67  QFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLRR 126
            FG R V+VVSS S  + C +KNDV+ ANRPR +SGKY+ YNYT+L    YGEHWRNLRR
Sbjct: 73  WFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRR 132

Query: 127 ISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAEND---VVDMRTEFFEVMFNITMRMIA 186
           I++L++LS+HR+ + S IR DE + LI RL +  +     +++ +  +++ FN  MRMI+
Sbjct: 133 ITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMRMIS 192

Query: 187 GKRYYGEDV-AAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRELQ 246
           GKRYYGED   +  +EA +FR++  E  +LSG  N  DF+ +++++   + +E RL+++ 
Sbjct: 193 GKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDF-ENLEKRLKDIS 252

Query: 247 IKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRGLM 306
            K D +++ LIEEHR K   A             TMI+ LL+LQ  +P+YY D+ I+GL 
Sbjct: 253 GKTDAFLRGLIEEHRTKKERA------------NTMIDHLLNLQDSQPEYYTDQIIKGLA 312

Query: 307 LVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYLRS 366
           L +LLAGT+ S  T+EW +S LLNHP  L++ + E+D  VG+ +RL++ESDL  L YL++
Sbjct: 313 LAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQ-DRLVDESDLPKLTYLKN 372

Query: 367 LIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFNPD 426
           +I+ETLR+Y P PLL+PH +S++C++GG+ VP  T++ +N WAI  DP +W E   F P+
Sbjct: 373 VINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPE 432

Query: 427 RFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEGGGL 486
           RF   GE  K + FG GRR CPGEGL +R I + +  LIQCF+W+ ++G+ ID++E  G 
Sbjct: 433 RFEKKGELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGF 490

Query: 487 TLPKALPLRTLCRPR 498
           TL K +PL+ +C+ R
Sbjct: 493 TLTKLVPLKAMCKSR 490

[Close]

BLAST of Cucsat.G20977 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 1.7e-120
Identity = 232/502 (46.22%), Postives = 326/502 (64.94%), Query Frame = 0


Query: 5   FLHLLI-LFLLQIFTNHFLH---RIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 64
           +L L+I LF L I    F +   + +NLPP P   LPIIG L+ LK PLH T   +S+++
Sbjct: 5   YLSLIISLFFLIITLKVFFNTSRKFKNLPPGP-QCLPIIGNLHQLKQPLHHTFHTLSQKY 64

Query: 65  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 124
           G I    FG R V+VVSS + A++C +KND++ ANRP  ++GKY+GYN T++  +PYG+H
Sbjct: 65  GQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDH 124

Query: 125 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRL----YNAENDVVDMRTEFFEVMFN 184
           WRNLRRI S+EILSSHRL +   IR DE+  LI++L    YN   + V++R  F E+ FN
Sbjct: 125 WRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTE-VELRPMFSEMTFN 184

Query: 185 ITMRMIAGKRYYGEDV-AAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIE 244
             MRM++GKRYYG D   +  EEA  FR I +E   L G  N+GDFL  ++W     G+E
Sbjct: 185 TIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFD-GLE 244

Query: 245 NRLRELQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKD 304
            RL+++  + D ++Q LI+EHR    N+             TMI+ LL+ QQ +P+YY D
Sbjct: 245 KRLKKISKRTDAFLQGLIDEHRFGKRNS------------NTMIDHLLTQQQSQPEYYTD 304

Query: 305 EYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLT 364
           + I+GLM+V+LLAGT+ S  T+EW +S LLNHP  +++A+ E+D  +G  +R ++E D++
Sbjct: 305 QIIKGLMVVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGH-DRQVDEHDIS 364

Query: 365 HLPYLRSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVE 424
            LPYL+S+++ETLR++   PLL+PH SSED  +GG+++P  T+L VN W I  DP +W +
Sbjct: 365 KLPYLQSIVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSD 424

Query: 425 PRKFNPDRFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECID 484
           P  F P+RF  +GE  K + FG GRR CPGE L  R  GL +G LIQCFEW+ +  E ID
Sbjct: 425 PTCFKPERFEKEGEVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKID 484

Query: 485 MSEGGGLTLPKALPLRTLCRPR 498
           M E  G+T  K   L  +C+ R
Sbjct: 485 MVEAKGITAGKKTSLNAMCKVR 490

[Close]

BLAST of Cucsat.G20977 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 6.3e-120
Identity = 230/500 (46.00%), Postives = 323/500 (64.60%), Query Frame = 0


Query: 6   LHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRHGPIVF 65
           L L  +  ++I       R++NLPP P  ++PIIG L+ LK PLHRT + +S+ +G I  
Sbjct: 8   LSLSFIITIKILLKITSRRLKNLPPGP-PTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFS 67

Query: 66  FQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLR 125
             FG R V+VVSSPS A +C +KND+I ANRPR ++GKY+ YNYT+L  A YG+HWRNLR
Sbjct: 68  LWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLR 127

Query: 126 RISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDV---------VDMRTEFFEVMFN 185
           RI+++++LS++RL +   +R DE   LI++L     DV         V++R    E+ FN
Sbjct: 128 RITTIDVLSNNRLNSFLGVRRDETNRLIQKLL---KDVVSEGFGFTKVELRPRLTEMTFN 187

Query: 186 ITMRMIAGKRYYGED-VAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIE 245
             MRMI+GKRYYG+D   +  EEA +FREI  E   L G  N GDFL +++ V L   +E
Sbjct: 188 AMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLD-NLE 247

Query: 246 NRLRELQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKD 305
            R + +  + + +++ LIEEHR+   N +S           TMI+ LL L + +P+YY D
Sbjct: 248 KRCKRIAKRSNAFLEGLIEEHRR--GNIHSDGG--------TMIDHLLKLSESQPEYYSD 307

Query: 306 EYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLT 365
             I+GL+  +LLAGT+ S  T+EW++S LLNHP  L++A+ E+D  +G+ N+L++E DL+
Sbjct: 308 HLIKGLIQGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGK-NKLVDEQDLS 367

Query: 366 HLPYLRSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVE 425
            LPYL+++I ETLR++PP PLL+PH SSEDC +G F+VP  T++  NVW I  DP  W +
Sbjct: 368 KLPYLQNIISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWND 427

Query: 426 PRKFNPDRFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECID 485
              F P+RF  + E  K M FG GRR CPG  L  R +G  VG LIQCFEWE    E +D
Sbjct: 428 ALSFKPERFEKEEEVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLD 487

Query: 486 MSEGGGLTLPKALPLRTLCR 496
           M EG G+T+P  +PLR +C+
Sbjct: 488 MMEGKGITMPMKIPLRAMCK 491

[Close]

BLAST of Cucsat.G20977 vs. ExPASy Swiss-Prot
Match: Q0WTF4 (Cytochrome P450 81F3 OS=Arabidopsis thaliana OX=3702 GN=CYP81F3 PE=2 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 2.4e-119
Identity = 228/498 (45.78%), Postives = 321/498 (64.46%), Query Frame = 0


Query: 9   LILFLL--QIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRHGPIVFF 68
           L LFLL  ++F      R  NLPPSP  SLPI+G   LLK P+HR    +S+ HGPI   
Sbjct: 10  LALFLLAYKLFFTSKTKRF-NLPPSPPYSLPILGHHNLLKPPVHRLFHRLSKTHGPIFSL 69

Query: 69  QFGFRSVLVVSSPSAAEDCLS-KNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLR 128
           QFG R  +V+SS S A  C + +ND+I +NRP  ++ KY+ YNYT++  APYG+HWRNLR
Sbjct: 70  QFGSRRAVVISSSSLATQCFTGQNDIILSNRPCFLTAKYVAYNYTTVGTAPYGDHWRNLR 129

Query: 129 RISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMRMIAGK 188
           RI SLEILSS+RL     IR DE+  ++ RL    N  +++     ++ FN  +RM+ GK
Sbjct: 130 RICSLEILSSNRLTNFLHIRKDEIHRMLTRLSRDVNKEIELEPLLSDLTFNNIVRMVTGK 189

Query: 189 RYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRELQIKR 248
           RYYG++V    EEA  F+++  +    SG  + GD+L  +K  G S   E +++ L    
Sbjct: 190 RYYGDEV-HNEEEANVFKKLVADINDCSGARHPGDYLPFMKMFGGS--FEKKVKALAEAM 249

Query: 249 DDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRGLMLVL 308
           D+ +Q L+EE ++  +               TM+  LLSLQQ EP+YY D  I+GLML +
Sbjct: 250 DEILQRLLEECKRDKDG-------------NTMVNHLLSLQQNEPEYYTDVTIKGLMLGM 309

Query: 309 LLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYLRSLIH 368
           ++AGT+ S  T+EW +S LLNHP +L++A++EID+ +G+  RL++E D+ +LPYL++++ 
Sbjct: 310 MIAGTDTSAVTLEWAMSSLLNHPEALEKAKLEIDEKIGQ-ERLIDEPDIANLPYLQNIVS 369

Query: 369 ETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFNPDRF- 428
           ET R+YP  PLL+P   +ED  VGG+ VP GTM+ VN WAI  DP +W EP KF P+RF 
Sbjct: 370 ETFRLYPAAPLLVPRSPTEDIKVGGYDVPRGTMVMVNAWAIHRDPELWNEPEKFKPERFN 429

Query: 429 GGDGEG-----FKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEG 488
           GG+G G      K MPFG GRR CPG GLG +++ L +GSLIQCF+W+ ++GE IDM+E 
Sbjct: 430 GGEGGGRGEDVHKLMPFGNGRRSCPGAGLGQKIVTLALGSLIQCFDWQKVNGEAIDMTET 489

Query: 489 GGLTLPKALPLRTLCRPR 498
            G+ + K +PL  LC+ R
Sbjct: 490 PGMAMRKKIPLSALCQSR 489

[Close]

BLAST of Cucsat.G20977 vs. NCBI nr
Match: XP_004141396.1 (cytochrome P450 81Q32 [Cucumis sativus] >XP_011654142.1 cytochrome P450 81Q32 [Cucumis sativus] >XP_031739532.1 cytochrome P450 81Q32 [Cucumis sativus] >KGN55248.1 hypothetical protein Csa_012192 [Cucumis sativus])

HSP 1 Score: 1019 bits (2636), Expect = 0.0
Identity = 507/507 (100.00%), Postives = 507/507 (100.00%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH
Sbjct: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR
Sbjct: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE
Sbjct: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300
           LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG
Sbjct: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300

Query: 301 LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL 360
           LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL
Sbjct: 301 LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL 360

Query: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN 420
           RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN
Sbjct: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN 420

Query: 421 PDRFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEGG 480
           PDRFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEGG
Sbjct: 421 PDRFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEGG 480

Query: 481 GLTLPKALPLRTLCRPRSNATHLLSQI 507
           GLTLPKALPLRTLCRPRSNATHLLSQI
Sbjct: 481 GLTLPKALPLRTLCRPRSNATHLLSQI 507

[Close]

BLAST of Cucsat.G20977 vs. NCBI nr
Match: XP_008452618.1 (PREDICTED: cytochrome P450 81E8-like [Cucumis melo])

HSP 1 Score: 948 bits (2450), Expect = 0.0
Identity = 473/508 (93.11%), Postives = 488/508 (96.06%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           MVI FL+LLILF+LQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLK PLHRTLSEISRRH
Sbjct: 31  MVISFLYLLILFILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRH 90

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPI+FFQFGFR VLVVSSPSA EDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH
Sbjct: 91  GPILFFQFGFRPVLVVSSPSAVEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 150

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEILSSHRLQTLSSIRVDEVR LIRRLYNAENDVVDMRTEFFE+MFNI MR
Sbjct: 151 WRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAENDVVDMRTEFFELMFNIMMR 210

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGE V A SEE  RF+EIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE
Sbjct: 211 MIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 270

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSS-IQTDAKKTMIEVLLSLQQKEPQYYKDEYIR 300
           LQI+RDDWMQSLIEEHRKKM+NA+SSSSS IQ DAKKTMIEVLLSLQQ EPQ YKDEYIR
Sbjct: 271 LQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQNYKDEYIR 330

Query: 301 GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPY 360
           GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSL+RAQMEID+VVG+TNRLLEESDLTHLPY
Sbjct: 331 GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDLTHLPY 390

Query: 361 LRSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKF 420
           LRSLIHETLRMYPPGPLLIPHESS+DCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEP KF
Sbjct: 391 LRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPEKF 450

Query: 421 NPDRFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEG 480
           NPDRF  DGEGFKWMPFGAGRRRCPGE LGLRV+GLV+GSLIQCFEWESM G+CIDMSEG
Sbjct: 451 NPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCIDMSEG 510

Query: 481 GGLTLPKALPLRTLCRPRSNATHLLSQI 507
           GGLTLPKALPLRTLCRPRSNATHLLSQI
Sbjct: 511 GGLTLPKALPLRTLCRPRSNATHLLSQI 538

[Close]

BLAST of Cucsat.G20977 vs. NCBI nr
Match: XP_038899302.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 914 bits (2362), Expect = 0.0
Identity = 448/507 (88.36%), Postives = 476/507 (93.89%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           M IPFL+LL+LF+LQIFTNHFLHRIRNLPPSP+LSLPIIGQLYLLK+PLHRTLSEI+ RH
Sbjct: 5   MAIPFLYLLVLFILQIFTNHFLHRIRNLPPSPYLSLPIIGQLYLLKSPLHRTLSEIAHRH 64

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPI+FF+FGFR VLVVSSPSAAEDCLSKNDV+FANRPRL SGKYLGYNYTSLLWAPYGEH
Sbjct: 65  GPILFFRFGFRQVLVVSSPSAAEDCLSKNDVVFANRPRLFSGKYLGYNYTSLLWAPYGEH 124

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEILS HRLQTLSSIRVDEVRTLIRRLYNA+NDVVDMRTEFFE+MFNI MR
Sbjct: 125 WRNLRRISSLEILSKHRLQTLSSIRVDEVRTLIRRLYNAKNDVVDMRTEFFELMFNIMMR 184

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGEDVAA +EE GRFREIQEETFRLS KTNLGDFLRVVKWVGLSKG+EN+LRE
Sbjct: 185 MIAGKRYYGEDVAADTEEVGRFREIQEETFRLSSKTNLGDFLRVVKWVGLSKGLENKLRE 244

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300
           LQ+KRD WMQSLIEEHRK+MNN   +S+S+Q DAKKTMIEVLLSLQQ EP YYKDE+IRG
Sbjct: 245 LQMKRDVWMQSLIEEHRKQMNN---TSTSLQNDAKKTMIEVLLSLQQMEPHYYKDEFIRG 304

Query: 301 LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL 360
           LMLVLLLAGTEGSINTMEWLLSLLLNHP  LQRAQMEIDDVVGR+NR+LEESDL HLPYL
Sbjct: 305 LMLVLLLAGTEGSINTMEWLLSLLLNHPDCLQRAQMEIDDVVGRSNRILEESDLAHLPYL 364

Query: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN 420
           RSLIHETLRMYPPGPLLIPHESSEDCHVGGF VPAGTMLFVNVWAIQNDPTVWVEP  FN
Sbjct: 365 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFCVPAGTMLFVNVWAIQNDPTVWVEPGNFN 424

Query: 421 PDRFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEGG 480
           P+RFG +GEGFKWMPFGAGRRRCPGEGLGLRV+GL VGSLIQCF+WES+ GEC+DM EGG
Sbjct: 425 PERFGSEGEGFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFDWESVGGECVDMREGG 484

Query: 481 GLTLPKALPLRTLCRPRSNATHLLSQI 507
           GLTLPKALPL  LCRPRSN  HLL QI
Sbjct: 485 GLTLPKALPLHALCRPRSNVAHLLPQI 508

[Close]

BLAST of Cucsat.G20977 vs. NCBI nr
Match: KAG7024285.1 (Isoflavone 2'-hydroxylase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 860 bits (2222), Expect = 2.03e-312
Identity = 431/509 (84.68%), Postives = 453/509 (89.00%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           M I FL LLIL +L IFTN FLHRIRNLPPSPF SLPIIGQLYLLK PLHRTLS+ISRRH
Sbjct: 1   MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPI+FF FGFR VLVVSSPSAAE+CLSKND++FANRP L+ GKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEI S+HRLQTLSSIRVDEVRTLIRRLYN +NDVVDMRTEFFE+MFNI MR
Sbjct: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMRTEFFELMFNIMMR 180

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGED AA SEEA RFREIQEETFRLS KTNLGDFLR+VKW GLSKG+ENR++E
Sbjct: 181 MIAGKRYYGED-AAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKE 240

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300
           LQ KRD WMQSLIEEHRK+ N           DAKKTMIEVLLSLQQKEP YYKDEYIRG
Sbjct: 241 LQSKRDAWMQSLIEEHRKQSNK----------DAKKTMIEVLLSLQQKEPHYYKDEYIRG 300

Query: 301 LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL 360
           LMLVLLLAGTEGSINTMEWLLSLLLN+P  LQRAQ EIDDVVGR+NR+LEESDL HLPYL
Sbjct: 301 LMLVLLLAGTEGSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYL 360

Query: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN 420
           RSLIHETLRMYPPGPLLIPHESSEDCHVGGF VPAGT LFVNVWAIQNDPTVWVEP KFN
Sbjct: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVEPEKFN 420

Query: 421 PDRFGGDGEG--FKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSE 480
           P RFG +GEG  FKWMPFGAGRRRCPGEGLGLRV+GL VGSLIQCFEWES+ GECIDM E
Sbjct: 421 PGRFGSEGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMRE 480

Query: 481 GGGLTLPKALPLRTLCRPRSNATHLLSQI 507
           G GLTLPKA+PL  LCRPRS   HLL QI
Sbjct: 481 GVGLTLPKAVPLHALCRPRSKVAHLLPQI 498

[Close]

BLAST of Cucsat.G20977 vs. NCBI nr
Match: XP_023535659.1 (isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 858 bits (2216), Expect = 1.67e-311
Identity = 428/509 (84.09%), Postives = 453/509 (89.00%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           M I FL LLIL +L IFTN FLHRIRNLPPSPF SLPIIGQLYLLK PLHRTLS+ISRRH
Sbjct: 1   MAITFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPI+FF FGFR VLVVSSPSAAE+CLSKND++FANRP L+ GKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRR+SSLEI S+HRLQTLSSIRVDEVRTLIRRLYN ENDVVDMRTEFFE+MFNI MR
Sbjct: 121 WRNLRRVSSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTENDVVDMRTEFFELMFNIMMR 180

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGED AA SEEA RFREIQEETFRLS KTNLGDFLR+VKW GLSKG+ENR++E
Sbjct: 181 MIAGKRYYGED-AAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKE 240

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300
           LQ KRD WMQSLIEEHRK+             DAKKTMIEVLLSLQQKEP+YYKDEYIRG
Sbjct: 241 LQSKRDAWMQSLIEEHRKQSKK----------DAKKTMIEVLLSLQQKEPRYYKDEYIRG 300

Query: 301 LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL 360
           LMLVLLLAGTEGSINTMEWLLSLLLN+P  LQRAQ+EIDDVVGR+NR+LEESDL HLPYL
Sbjct: 301 LMLVLLLAGTEGSINTMEWLLSLLLNNPECLQRAQLEIDDVVGRSNRILEESDLAHLPYL 360

Query: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN 420
           RSLIHETLRMYPPGPLLIPHESS DCHVGGF VPAGT LFVNVWAIQNDPTVWV+P KFN
Sbjct: 361 RSLIHETLRMYPPGPLLIPHESSADCHVGGFRVPAGTTLFVNVWAIQNDPTVWVDPEKFN 420

Query: 421 PDRFGGDGEG--FKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSE 480
           P RFG +GEG  FKWMPFGAGRRRCPGEGLGLRV+GL VGSLIQCFEWES+ GECIDM E
Sbjct: 421 PGRFGSEGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMRE 480

Query: 481 GGGLTLPKALPLRTLCRPRSNATHLLSQI 507
           G GLTLPKA+PL  LCRPRS   HLL QI
Sbjct: 481 GVGLTLPKAVPLHALCRPRSKVAHLLPQI 498

[Close]

BLAST of Cucsat.G20977 vs. ExPASy TrEMBL
Match: A0A0A0L0F1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G642290 PE=3 SV=1)

HSP 1 Score: 1019 bits (2636), Expect = 0.0
Identity = 507/507 (100.00%), Postives = 507/507 (100.00%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH
Sbjct: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR
Sbjct: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE
Sbjct: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300
           LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG
Sbjct: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300

Query: 301 LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL 360
           LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL
Sbjct: 301 LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL 360

Query: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN 420
           RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN
Sbjct: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN 420

Query: 421 PDRFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEGG 480
           PDRFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEGG
Sbjct: 421 PDRFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEGG 480

Query: 481 GLTLPKALPLRTLCRPRSNATHLLSQI 507
           GLTLPKALPLRTLCRPRSNATHLLSQI
Sbjct: 481 GLTLPKALPLRTLCRPRSNATHLLSQI 507

[Close]

BLAST of Cucsat.G20977 vs. ExPASy TrEMBL
Match: A0A1S3BVF3 (cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103493585 PE=3 SV=1)

HSP 1 Score: 948 bits (2450), Expect = 0.0
Identity = 473/508 (93.11%), Postives = 488/508 (96.06%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           MVI FL+LLILF+LQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLK PLHRTLSEISRRH
Sbjct: 31  MVISFLYLLILFILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRH 90

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPI+FFQFGFR VLVVSSPSA EDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH
Sbjct: 91  GPILFFQFGFRPVLVVSSPSAVEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 150

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEILSSHRLQTLSSIRVDEVR LIRRLYNAENDVVDMRTEFFE+MFNI MR
Sbjct: 151 WRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAENDVVDMRTEFFELMFNIMMR 210

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGE V A SEE  RF+EIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE
Sbjct: 211 MIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 270

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSS-IQTDAKKTMIEVLLSLQQKEPQYYKDEYIR 300
           LQI+RDDWMQSLIEEHRKKM+NA+SSSSS IQ DAKKTMIEVLLSLQQ EPQ YKDEYIR
Sbjct: 271 LQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQNYKDEYIR 330

Query: 301 GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPY 360
           GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSL+RAQMEID+VVG+TNRLLEESDLTHLPY
Sbjct: 331 GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDLTHLPY 390

Query: 361 LRSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKF 420
           LRSLIHETLRMYPPGPLLIPHESS+DCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEP KF
Sbjct: 391 LRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPEKF 450

Query: 421 NPDRFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEG 480
           NPDRF  DGEGFKWMPFGAGRRRCPGE LGLRV+GLV+GSLIQCFEWESM G+CIDMSEG
Sbjct: 451 NPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCIDMSEG 510

Query: 481 GGLTLPKALPLRTLCRPRSNATHLLSQI 507
           GGLTLPKALPLRTLCRPRSNATHLLSQI
Sbjct: 511 GGLTLPKALPLRTLCRPRSNATHLLSQI 538

[Close]

BLAST of Cucsat.G20977 vs. ExPASy TrEMBL
Match: A0A6J1FED9 (isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111443311 PE=3 SV=1)

HSP 1 Score: 857 bits (2213), Expect = 2.31e-311
Identity = 429/509 (84.28%), Postives = 452/509 (88.80%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           M I FL LLIL +L IFTN FLHRIRNLPPSPF SLPIIGQLYLLK PLHRTLS+ISRRH
Sbjct: 1   MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPI+FF FGFR VLVVSSPSAAE+CLSKND++FANRP L+ GKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEI S+HRLQTLSSIRVDEVRTLIRRLYN +NDVVDMRTEFFE+MFNI MR
Sbjct: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMRTEFFELMFNIMMR 180

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGED AA SEEA RFREIQEETFRLS KTNLGDFLR+VKW GLSKG+ENR++E
Sbjct: 181 MIAGKRYYGED-AAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKE 240

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300
           LQ KRD WMQSLIEEHRK+             DAKKTMIEVLLSLQQKEP YYKDEYIRG
Sbjct: 241 LQSKRDAWMQSLIEEHRKQSKK----------DAKKTMIEVLLSLQQKEPHYYKDEYIRG 300

Query: 301 LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL 360
           LMLVLLLAGTEGSINTMEWLLSLLLN+P  LQRAQ EIDDVVGR+NR+LEESDL HLPYL
Sbjct: 301 LMLVLLLAGTEGSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYL 360

Query: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN 420
           RSLIHETLRMYPPGPLLIPHESSEDCHVGGF VPAGT LFVNVWAIQNDPTVWV+P KFN
Sbjct: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVDPEKFN 420

Query: 421 PDRFGGDGEG--FKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSE 480
           P RFG +GEG  FKWMPFGAGRRRCPGEGLGLRV+GL VGSLIQCFEWES+ GECIDM E
Sbjct: 421 PGRFGSEGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMRE 480

Query: 481 GGGLTLPKALPLRTLCRPRSNATHLLSQI 507
           G GLTLPKA+PL  LCRPRS   HLL QI
Sbjct: 481 GVGLTLPKAVPLHALCRPRSKVAHLLPQI 498

[Close]

BLAST of Cucsat.G20977 vs. ExPASy TrEMBL
Match: A0A6J1INS3 (isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111477207 PE=3 SV=1)

HSP 1 Score: 853 bits (2205), Expect = 3.82e-310
Identity = 427/509 (83.89%), Postives = 451/509 (88.61%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           M I FL LLILF+L IFTN FLHRIRNLPPSPF SLPIIGQLYLLK PLHRTL +ISRRH
Sbjct: 1   MAITFLCLLILFILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLLDISRRH 60

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPI+FF FGFR VLVVSSPSAAE+CLSKND++FANRP L+ GKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEI S+HRLQTLSSIRVDEVRTLIRRLYN +NDVVDM+TEFFE+MFNI MR
Sbjct: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMKTEFFELMFNIMMR 180

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGEDVA  SEEA RFREIQEETFRLS KTNLGDFLR+VKW GLSKG+ENR++E
Sbjct: 181 MIAGKRYYGEDVAE-SEEAERFREIQEETFRLSSKTNLGDFLRMVKWAGLSKGLENRMKE 240

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300
           LQ KRD WMQSLIEEHRK+             DAKKTMIEVLLSLQQKEP YYKDEYIRG
Sbjct: 241 LQSKRDAWMQSLIEEHRKQSKK----------DAKKTMIEVLLSLQQKEPHYYKDEYIRG 300

Query: 301 LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL 360
           LMLVLLLAGTEGSINTMEWLLSLLLN+P  LQRAQ EIDDVVGR+NR+LEESDL HLPYL
Sbjct: 301 LMLVLLLAGTEGSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYL 360

Query: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN 420
           RSLIHETLRMYPPGPLLIPHESS DCHVGGF VPAGT LFVNVWAIQNDPTVWV+P KFN
Sbjct: 361 RSLIHETLRMYPPGPLLIPHESSADCHVGGFRVPAGTTLFVNVWAIQNDPTVWVDPEKFN 420

Query: 421 PDRFGGDGEG--FKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSE 480
           P RFG +GEG  FKWMPFGAGRRRCPGEGLGLRV+GL VGSLIQCFEWES+ GECIDM E
Sbjct: 421 PGRFGSEGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMRE 480

Query: 481 GGGLTLPKALPLRTLCRPRSNATHLLSQI 507
           G GLTLPKA+PL  LCRPRS   HLL QI
Sbjct: 481 GVGLTLPKAVPLHALCRPRSKVAHLLPQI 498

[Close]

BLAST of Cucsat.G20977 vs. ExPASy TrEMBL
Match: A0A7J0EHR3 (Cytochrome P450 superfamily protein OS=Actinidia rufa OX=165716 GN=Acr_04g0007630 PE=3 SV=1)

HSP 1 Score: 644 bits (1662), Expect = 1.70e-227
Identity = 321/495 (64.85%), Postives = 395/495 (79.80%), Query Frame = 0


Query: 16  IFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRHGPIVFFQFGFRSVLV 75
           I   HFLH+IRNLPPSPF +LPI G LYLLKTP+HRTLS IS  HGPI+  +FGFR VL+
Sbjct: 16  IIAKHFLHKIRNLPPSPFPALPIFGHLYLLKTPIHRTLSNISAHHGPILLLRFGFRPVLL 75

Query: 76  VSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLRRISSLEILSS 135
           VSSPSAAE+CLSKND++FANRPRL++GK+LG NY +L WAPYG  WRNLRRI+++EILSS
Sbjct: 76  VSSPSAAEECLSKNDIVFANRPRLLAGKHLGQNYNTLSWAPYGARWRNLRRIAAVEILSS 135

Query: 136 HRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMRMIAGKRYYGEDVAAY 195
           HRLQTLSSIR DEVR+LIRRL    ++ VDM+  FFE+M N+TMRMIAGK YYGEDVA  
Sbjct: 136 HRLQTLSSIRADEVRSLIRRLARG-SEGVDMKAAFFELMLNVTMRMIAGKTYYGEDVAE- 195

Query: 196 SEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRELQIKRDDWMQSLIEE 255
           +E A RFREI  ETF L+G +N GDFL V+KW+G S G+E RL  L+ KRD++MQ +IEE
Sbjct: 196 AEAARRFREIVTETFVLAGASNFGDFLPVMKWIGAS-GLEKRLIALREKRDEFMQEMIEE 255

Query: 256 HRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRGLMLVLLLAGTEGSIN 315
            R+++    S       + KKTM+EVLLSLQ+ EP++Y D+ IRG+M VLL AGT+ S+ 
Sbjct: 256 QRRRIGGGGSE------EKKKTMVEVLLSLQETEPEHYTDQMIRGMMSVLLAAGTDTSVG 315

Query: 316 TMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYLRSLIHETLRMYPPGP 375
           TMEW LSLLLN P++LQ+AQ EID  VG+ +RL++ESD+  LPYLR +I+ET+RMYP GP
Sbjct: 316 TMEWALSLLLNQPYALQKAQTEIDKQVGQ-HRLIDESDIADLPYLRCIINETMRMYPAGP 375

Query: 376 LLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFNPDRFGG-DG--EGFK 435
           LL+PHESSEDC VGGFH+P GTML VN+WAIQNDP +WVEP KF P+RF G +G  +GFK
Sbjct: 376 LLVPHESSEDCMVGGFHIPRGTMLLVNMWAIQNDPKIWVEPNKFKPERFEGLEGARDGFK 435

Query: 436 WMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEGGGLTLPKALPLRT 495
           ++PFG+GRR CPGEGL LR++GL +GSLIQCF+WE +  E +DM+EG GLTLPKA PL  
Sbjct: 436 FVPFGSGRRVCPGEGLALRMVGLALGSLIQCFDWERVGKEMVDMTEGTGLTLPKARPLMA 495

Query: 496 LCRPRSNATHLLSQI 507
            CRPR    ++LSQI
Sbjct: 496 KCRPRLTMVNILSQI 500

The following BLAST results are available for this feature:

BLAST of Cucsat.G20977 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
W8JMU7	3.4e-134	48.53	Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1	[more]
P93147	3.0e-122	45.25	Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2	[more]
Q6WNQ8	1.7e-120	46.22	Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1	[more]
Q6WNQ9	6.3e-120	46.00	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P...	[more]
Q0WTF4	2.4e-119	45.78	Cytochrome P450 81F3 OS=Arabidopsis thaliana OX=3702 GN=CYP81F3 PE=2 SV=1	[more]

BLAST of Cucsat.G20977 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004141396.1	0.0	100.00	cytochrome P450 81Q32 [Cucumis sativus] >XP_011654142.1 cytochrome P450 81Q32 [C...	[more]
XP_008452618.1	0.0	93.11	PREDICTED: cytochrome P450 81E8-like [Cucumis melo]	[more]
XP_038899302.1	0.0	88.36	cytochrome P450 81Q32-like [Benincasa hispida]	[more]
KAG7024285.1	2.03e-312	84.68	Isoflavone 2'-hydroxylase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023535659.1	1.67e-311	84.09	isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cucsat.G20977 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L0F1	0.0	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G642290 PE=3 SV=1	[more]
A0A1S3BVF3	0.0	93.11	cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103493585 PE=3 SV=1	[more]
A0A6J1FED9	2.31e-311	84.28	isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111443311 PE=...	[more]
A0A6J1INS3	3.82e-310	83.89	isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111477207 PE=3 ...	[more]
A0A7J0EHR3	1.70e-227	64.85	Cytochrome P450 superfamily protein OS=Actinidia rufa OX=165716 GN=Acr_04g000763...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 45..63 score: 34.78 coord: 86..110 score: 35.4 coord: 117..127 score: 57.48 coord: 1..27 score: 36.21 coord: 127..150 score: 33.8
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 46..57 score: 40.54 coord: 118..127 score: 60.07 coord: 10..23 score: 33.02 coord: 127..138 score: 31.36
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 1..170 e-value: 6.0E-53 score: 180.2
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..189 e-value: 6.7E-61 score: 208.4
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 1..181
None	No IPR available	PANTHER	PTHR47947	CYTOCHROME P450 82C3-RELATED	coord: 1..190
None	No IPR available	PANTHER	PTHR47947:SF17	CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 5	coord: 1..190
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 120..129

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G20977.T4	Cucsat.G20977.T4	mRNA
Cucsat.G20977.T1	Cucsat.G20977.T1	mRNA
Cucsat.G20977.T2	Cucsat.G20977.T2	mRNA
Cucsat.G20977.T11	Cucsat.G20977.T11	mRNA
Cucsat.G20977.T12	Cucsat.G20977.T12	mRNA
Cucsat.G20977.T6	Cucsat.G20977.T6	mRNA
Cucsat.G20977.T8	Cucsat.G20977.T8	mRNA
Cucsat.G20977.T3	Cucsat.G20977.T3	mRNA
Cucsat.G20977.T10	Cucsat.G20977.T10	mRNA
Cucsat.G20977.T5	Cucsat.G20977.T5	mRNA
Cucsat.G20977.T7	Cucsat.G20977.T7	mRNA
Cucsat.G20977.T13	Cucsat.G20977.T13	mRNA
Cucsat.G20977.T14	Cucsat.G20977.T14	mRNA
Cucsat.G20977.T9	Cucsat.G20977.T9	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0098542	defense response to other organism
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen