Cucsat.G20977.T4 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G20977.T4
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	cytochrome P450 81E8-like
Location	ctg907: 474437 .. 477426 (+)
RNA-Seq Expression	Cucsat.G20977.T4
Synteny	Cucsat.G20977.T4

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAAATTAACTGCAAATGAAACAAAATTCAGTTTAAGAATGGGGAATTATCTGTCTCACTTCACTGTCTACATCAACGATTGGTTATTCAAAGTACACTCAGTGGATTTGAGATAAATTTTCCCTTTCCATTTTGCCCATAAAATGACCATTCTGCCCTCACTGGGTTAATTGATTTCTTTTCTCTTTCAGGATATTTAAGGAAACTTGAAAGGAATTTGTGTATAAGATTTTTTCTGGCTCTTCTTCGTGACTTGAGGTTTTATGGGTTTTTGGTTTTCACCCAACCAAGAAAGCTAATGTTTCTTCTTCGGATGGAAGATAGTTCATGGATGGTAACGTTTTTCTTCTTTAATTCATTTAACATTTTAGTTTTGAAATTTTCAGTATTGATACTGTTTTTGAGATTTTGTTGTAGAGGACATTCAGTTTACAATGAATAATGTAAATATTATATTCTTTGTTCATATATCTAAGTTTGTCTTCCAGGATTCTCTTTCACTAAAGATTCATTTTTTTCATTAATTATTTAGTTTCTATGAAATCTGAATCAATTACAAGGAATCACAGAAGCGATTACTCACGAGGTCAGTTTATCTAATTTTAATTTACGGAGATCCATATAACTTTTTTTTTCTTTCAATTATGGATTTCAATTTTGTTCATTTTCTTTCTACATTTTTTTTTGTCAATAATTTACTCTTTTACAACTTGTATATAATTGGCCGTATATTTCACCATACAAATTATATACAATTATATATCCATTATTTCTTTCTTCTTGTTTTAAAATAATATAACTTTCTATTAATTTTTTTATAGTTTAAACAAAATGTTAGATCACATAAATTTAGTTTTTTTAATTTCCATCAAACCCATTTTTTAGTTTAAACTTAGAGGTAAAAACAAATTTAATAGAATGTATAACACTCATCTATATTTAAGTATTATCTAAGTTTTTGAGTGATGATAAATTTAATATAACATCCAGTGTAACTAAATTGCCGAATTGACTTGCAGGTAAGAAAGCAACTTGAAAAATTTAAACGGAACTCAATGATACTTCGACCAACCCAACTAGCATGGTTATAATTTACAAATGATTCTCGCAGGTAAATTTCATGGTGATCCCATTCCTACATCTTCTGATTTTATTCTTACTTCAAATCTTCACAAATCACTTCCTTCATAGAATCCGAAATCTCCCTCCCAGCCCATTTCTTTCTCTACCCATTATAGGCCAACTATACCTCCTTAAAACACCCCTCCACCGGACCTTATCGGAGATCTCCCGCCGTCATGGCCCAATCGTCTTCTTCCAATTCGGTTTCCGTTCAGTCCTGGTCGTTTCATCTCCGTCGGCCGCCGAGGACTGCCTATCCAAAAACGACGTCATCTTCGCCAACCGACCTCGTCTCGTTAGCGGCAAATACCTCGGGTACAATTACACAAGCCTCCTGTGGGCACCGTACGGCGAGCATTGGCGAAACCTCCGTCGAATCTCGTCGTTAGAAATCCTTTCCAGCCACCGCCTCCAAACGCTTTCAAGTATTCGCGTGGATGAAGTCCGTACGCTGATCCGGAGACTGTACAACGCTGAAAACGACGTCGTAGACATGAGAACAGAATTTTTCGAAGTGATGTTCAATATCACGATGAGAATGATCGCCGGAAAACGATATTACGGGGAAGATGTGGCAGCGTACTCGGAGGAGGCGGGGAGATTTCGAGAGATACAAGAGGAGACGTTTCGATTAAGCGGCAAGACTAATTTGGGGGACTTTTTAAGGGTTGTGAAATGGGTTGGATTATCTAAAGGGATTGAAAATAGATTGAGGGAGTTACAGATTAAGAGAGATGATTGGATGCAGAGTTTGATTGAAGAACATCGGAAGAAAATGAATAATGCTTATAGTTCTTCTTCTTCTATTCAAACTGATGCTAAAAAGACTATGATTGAGGTTCTCCTCTCATTGCAACAAAAGGAACCTCAGTATTATAAGGACGAGTATATCAGAGGCCTCATGCTCGTGAGTTAACTTGATTTCTCTCTTAGTTATTCAAAAGACATAACCAAATAGATTTTGAAACACTATTTTAAACACATATGAAAATATATGTAATCCGACTCTATTTTTCCTTAAATCAACCAAATCGTCCCAAATGAAATTGGATTGTTGTAGGTGTTGCTATTAGCTGGAACGGAAGGTTCCATCAATACAATGGAGTGGCTTCTATCACTATTACTCAACCACCCACACTCTCTTCAACGAGCCCAAATGGAAATCGACGACGTAGTTGGAAGAACTAATCGGCTCCTGGAGGAATCAGATCTAACCCACCTTCCTTACCTCCGTAGCCTGATCCACGAAACTCTTCGAATGTACCCGCCAGGCCCATTACTGATTCCACACGAATCGTCAGAGGATTGCCACGTTGGAGGGTTCCATGTCCCAGCTGGCACAATGTTATTCGTCAACGTATGGGCCATTCAAAACGACCCCACCGTATGGGTGGAGCCGAGGAAATTTAACCCAGATAGATTCGGAGGGGATGGAGAGGGGTTCAAATGGATGCCATTTGGGGCTGGGCGGAGGAGGTGTCCCGGCGAGGGCTTGGGGCTTCGCGTCATTGGATTGGTTGTAGGGTCGCTGATTCAATGCTTTGAATGGGAGTCAATGGACGGAGAATGTATTGATATGAGCGAAGGTGGTGGATTGACCTTGCCGAAAGCTCTCCCCCTTCGCACCCTTTGCCGGCCACGTTCAAATGCCACACATTTGCTTTCCCAAATCTAATTCAAAATGCATGGTCTTGTTTATGTTGTTCAACAACAATTAATTCTGTACATAAATTTTACATATTTTAGTAGTAGTTGAAAACACCTACGTTATTTCCAATTTGTTGACGTCCTATTTTTCTTTTTTCTTTTGCCTCTTTGTTTACGTGATGATTGGTTGATGTCCTATATTTTAGTAGAAGCAAAAG

Coding sequence (CDS)

ATGGTGATCCCATTCCTACATCTTCTGATTTTATTCTTACTTCAAATCTTCACAAATCACTTCCTTCATAGAATCCGAAATCTCCCTCCCAGCCCATTTCTTTCTCTACCCATTATAGGCCAACTATACCTCCTTAAAACACCCCTCCACCGGACCTTATCGGAGATCTCCCGCCGTCATGGCCCAATCGTCTTCTTCCAATTCGGTTTCCGTTCAGTCCTGGTCGTTTCATCTCCGTCGGCCGCCGAGGACTGCCTATCCAAAAACGACGTCATCTTCGCCAACCGACCTCGTCTCGTTAGCGGCAAATACCTCGGGTACAATTACACAAGCCTCCTGTGGGCACCGTACGGCGAGCATTGGCGAAACCTCCGTCGAATCTCGTCGTTAGAAATCCTTTCCAGCCACCGCCTCCAAACGCTTTCAAGTATTCGCGTGGATGAAGTCCGTACGCTGATCCGGAGACTGTACAACGCTGAAAACGACGTCGTAGACATGAGAACAGAATTTTTCGAAGTGATGTTCAATATCACGATGAGAATGATCGCCGGAAAACGATATTACGGGGAAGATGTGGCAGCGTACTCGGAGGAGGCGGGGAGATTTCGAGAGATACAAGAGGAGACGTTTCGATTAAGCGGCAAGACTAATTTGGGGGACTTTTTAAGGGTTGTGAAATGGGTTGGATTATCTAAAGGGATTGAAAATAGATTGAGGGAGTTACAGATTAAGAGAGATGATTGGATGCAGAGTTTGATTGAAGAACATCGGAAGAAAATGAATAATGCTTATAGTTCTTCTTCTTCTATTCAAACTGATGCTAAAAAGACTATGATTGAGGTTCTCCTCTCATTGCAACAAAAGGAACCTCAGTATTATAAGGACGAGTATATCAGAGGCCTCATGCTCGTGAGTTAA

Protein sequence

MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRHGPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMRMIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRELQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRGLMLVS

Homology

[Close]

BLAST of Cucsat.G20977.T4 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 2.9e-64
Identity = 135/308 (43.83%), Postives = 206/308 (66.88%), Query Frame = 0


Query: 1   MVIPFLHLLILFL-LQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRR 60
           ++  FL +++L + L++F      R RNLPPSP L+LP+IG L+L+   LHR+L ++S++
Sbjct: 6   LLYTFLAVVLLSISLKLFP--VSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKK 65

Query: 61  HGPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGE 120
           +G +   Q G R VLVVSSP+AAE+C +KND++FANRP  + GKY+GYNYT+++ +PYGE
Sbjct: 66  YGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGE 125

Query: 121 HWRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAEND---VVDMRTEFFEVMFN 180
           HWRNLRR++++EI S+  L    SIR DEV+ L+  LY +       V+M+++  E+ FN
Sbjct: 126 HWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFN 185

Query: 181 ITMRMIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIEN 240
           +TMRM+AGKRY+G+DV   S+EA  FR +  E F  +G +N GDF+  ++W+   K  E 
Sbjct: 186 VTMRMVAGKRYFGQDVD--SDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDF-KNYEK 245

Query: 241 RLRELQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDE 300
           ++ ++  + D ++Q LI E R   NN              TMI+ LLSLQ+ +P+YY D+
Sbjct: 246 KVSKISQEMDAFLQRLIHESRINKNNV-------------TMIDHLLSLQESQPEYYTDQ 295

Query: 301 YIRGLMLV 305
            I+G+++V
Sbjct: 306 IIKGIIMV 295

[Close]

BLAST of Cucsat.G20977.T4 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 8.0e-62
Identity = 140/309 (45.31%), Postives = 196/309 (63.43%), Query Frame = 0


Query: 5   FLHLLI-LFLLQIFTNHFLH---RIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 64
           +L L+I LF L I    F +   + +NLPP P   LPIIG L+ LK PLH T   +S+++
Sbjct: 5   YLSLIISLFFLIITLKVFFNTSRKFKNLPPGP-QCLPIIGNLHQLKQPLHHTFHTLSQKY 64

Query: 65  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 124
           G I    FG R V+VVSS + A++C +KND++ ANRP  ++GKY+GYN T++  +PYG+H
Sbjct: 65  GQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDH 124

Query: 125 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRL----YNAENDVVDMRTEFFEVMFN 184
           WRNLRRI S+EILSSHRL +   IR DE+  LI++L    YN   + V++R  F E+ FN
Sbjct: 125 WRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTE-VELRPMFSEMTFN 184

Query: 185 ITMRMIAGKRYYGEDV-AAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIE 244
             MRM++GKRYYG D   +  EEA  FR I +E   L G  N+GDFL  ++W     G+E
Sbjct: 185 TIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFD-GLE 244

Query: 245 NRLRELQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKD 304
            RL+++  + D ++Q LI+EHR    N+             TMI+ LL+ QQ +P+YY D
Sbjct: 245 KRLKKISKRTDAFLQGLIDEHRFGKRNS------------NTMIDHLLTQQQSQPEYYTD 298

[Close]

BLAST of Cucsat.G20977.T4 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 231.1 bits (588), Expect = 1.7e-59
Identity = 130/301 (43.19%), Postives = 191/301 (63.46%), Query Frame = 0


Query: 7   HLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRHGPIVFF 66
           +L + F+  I       + +NLPP P  SLPIIG L+ LK PLHRT   +S ++G +   
Sbjct: 13  YLALFFIFNIVIR--ARKFKNLPPGP-PSLPIIGNLHHLKRPLHRTFKGLSEKYGHVFSL 72

Query: 67  QFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLRR 126
            FG R V+VVSS S  + C +KNDV+ ANRPR +SGKY+ YNYT+L    YGEHWRNLRR
Sbjct: 73  WFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRR 132

Query: 127 ISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAEND---VVDMRTEFFEVMFNITMRMIA 186
           I++L++LS+HR+ + S IR DE + LI RL +  +     +++ +  +++ FN  MRMI+
Sbjct: 133 ITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMRMIS 192

Query: 187 GKRYYGEDV-AAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRELQ 246
           GKRYYGED   +  +EA +FR++  E  +LSG  N  DF+ +++++   + +E RL+++ 
Sbjct: 193 GKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDF-ENLEKRLKDIS 252

Query: 247 IKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRGLM 304
            K D +++ LIEEHR K   A             TMI+ LL+LQ  +P+YY D+ I+GL 
Sbjct: 253 GKTDAFLRGLIEEHRTKKERA------------NTMIDHLLNLQDSQPEYYTDQIIKGLA 297

[Close]

BLAST of Cucsat.G20977.T4 vs. ExPASy Swiss-Prot
Match: Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 3.5e-57
Identity = 124/301 (41.20%), Postives = 191/301 (63.46%), Query Frame = 0


Query: 7   HLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRHGPIVFF 66
           +L I F++++       + +NLPP P  SLPIIG L+ LK PL+RT   ++ ++G ++  
Sbjct: 13  YLSIFFIIRLLFQS--RKFKNLPPGP-TSLPIIGNLHHLKRPLNRTFKALTEKYGNVISL 72

Query: 67  QFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLRR 126
            FG R V+VVSS S  ++C +KNDV+ ANRPR +SGKY+ YNYT+L    YGEHWRNLRR
Sbjct: 73  WFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRR 132

Query: 127 ISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAEND---VVDMRTEFFEVMFNITMRMIA 186
           I+SL++LS+HR+   + IR DE + LI++L    +     V++   FF++ FN  MRMI+
Sbjct: 133 ITSLDVLSNHRINNFAPIRRDETQRLIKKLAEDSSTKFAEVELTFRFFDMTFNNIMRMIS 192

Query: 187 GKRYYGEDV-AAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRELQ 246
           GKRYYG+D   +  +EA +FR++  E  +LSG  N  DF+ ++K++   + +E R++ + 
Sbjct: 193 GKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDF-ENLEKRVKRIG 252

Query: 247 IKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRGLM 304
            K D ++  L++E R K             +   TMI+ LL++Q+ +P+YY D  I+GL 
Sbjct: 253 EKNDVFLSGLLQEQRSK------------KERTNTMIDHLLNMQESQPEYYTDTIIKGLC 297

[Close]

BLAST of Cucsat.G20977.T4 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 4.6e-57
Identity = 132/307 (43.00%), Postives = 190/307 (61.89%), Query Frame = 0


Query: 6   LHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRHGPIVF 65
           L L  +  ++I       R++NLPP P  ++PIIG L+ LK PLHRT + +S+ +G I  
Sbjct: 8   LSLSFIITIKILLKITSRRLKNLPPGP-PTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFS 67

Query: 66  FQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLR 125
             FG R V+VVSSPS A +C +KND+I ANRPR ++GKY+ YNYT+L  A YG+HWRNLR
Sbjct: 68  LWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLR 127

Query: 126 RISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDV---------VDMRTEFFEVMFN 185
           RI+++++LS++RL +   +R DE   LI++L     DV         V++R    E+ FN
Sbjct: 128 RITTIDVLSNNRLNSFLGVRRDETNRLIQKLL---KDVVSEGFGFTKVELRPRLTEMTFN 187

Query: 186 ITMRMIAGKRYYGED-VAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIE 245
             MRMI+GKRYYG+D   +  EEA +FREI  E   L G  N GDFL +++ V L   +E
Sbjct: 188 AMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLD-NLE 247

Query: 246 NRLRELQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKD 303
            R + +  + + +++ LIEEHR+   N +S           TMI+ LL L + +P+YY D
Sbjct: 248 KRCKRIAKRSNAFLEGLIEEHRR--GNIHSDGG--------TMIDHLLKLSESQPEYYSD 299

[Close]

BLAST of Cucsat.G20977.T4 vs. NCBI nr
Match: XP_004141396.1 (cytochrome P450 81Q32 [Cucumis sativus] >XP_011654142.1 cytochrome P450 81Q32 [Cucumis sativus] >XP_031739532.1 cytochrome P450 81Q32 [Cucumis sativus] >KGN55248.1 hypothetical protein Csa_012192 [Cucumis sativus])

HSP 1 Score: 595 bits (1535), Expect = 3.36e-211
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH
Sbjct: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR
Sbjct: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE
Sbjct: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300
           LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG
Sbjct: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300

Query: 301 LMLV 304
           LMLV
Sbjct: 301 LMLV 304

[Close]

BLAST of Cucsat.G20977.T4 vs. NCBI nr
Match: XP_008452618.1 (PREDICTED: cytochrome P450 81E8-like [Cucumis melo])

HSP 1 Score: 544 bits (1402), Expect = 1.64e-190
Identity = 282/305 (92.46%), Postives = 290/305 (95.08%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           MVI FL+LLILF+LQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLK PLHRTLSEISRRH
Sbjct: 31  MVISFLYLLILFILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRH 90

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPI+FFQFGFR VLVVSSPSA EDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH
Sbjct: 91  GPILFFQFGFRPVLVVSSPSAVEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 150

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEILSSHRLQTLSSIRVDEVR LIRRLYNAENDVVDMRTEFFE+MFNI MR
Sbjct: 151 WRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAENDVVDMRTEFFELMFNIMMR 210

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGE V A SEE  RF+EIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE
Sbjct: 211 MIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 270

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSS-IQTDAKKTMIEVLLSLQQKEPQYYKDEYIR 300
           LQI+RDDWMQSLIEEHRKKM+NA+SSSSS IQ DAKKTMIEVLLSLQQ EPQ YKDEYIR
Sbjct: 271 LQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQNYKDEYIR 330

Query: 301 GLMLV 304
           GLMLV
Sbjct: 331 GLMLV 335

[Close]

BLAST of Cucsat.G20977.T4 vs. NCBI nr
Match: XP_038899302.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 533 bits (1372), Expect = 2.11e-186
Identity = 268/304 (88.16%), Postives = 288/304 (94.74%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           M IPFL+LL+LF+LQIFTNHFLHRIRNLPPSP+LSLPIIGQLYLLK+PLHRTLSEI+ RH
Sbjct: 5   MAIPFLYLLVLFILQIFTNHFLHRIRNLPPSPYLSLPIIGQLYLLKSPLHRTLSEIAHRH 64

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPI+FF+FGFR VLVVSSPSAAEDCLSKNDV+FANRPRL SGKYLGYNYTSLLWAPYGEH
Sbjct: 65  GPILFFRFGFRQVLVVSSPSAAEDCLSKNDVVFANRPRLFSGKYLGYNYTSLLWAPYGEH 124

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEILS HRLQTLSSIRVDEVRTLIRRLYNA+NDVVDMRTEFFE+MFNI MR
Sbjct: 125 WRNLRRISSLEILSKHRLQTLSSIRVDEVRTLIRRLYNAKNDVVDMRTEFFELMFNIMMR 184

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGEDVAA +EE GRFREIQEETFRLS KTNLGDFLRVVKWVGLSKG+EN+LRE
Sbjct: 185 MIAGKRYYGEDVAADTEEVGRFREIQEETFRLSSKTNLGDFLRVVKWVGLSKGLENKLRE 244

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300
           LQ+KRD WMQSLIEEHRK+MNN   +S+S+Q DAKKTMIEVLLSLQQ EP YYKDE+IRG
Sbjct: 245 LQMKRDVWMQSLIEEHRKQMNN---TSTSLQNDAKKTMIEVLLSLQQMEPHYYKDEFIRG 304

Query: 301 LMLV 304
           LMLV
Sbjct: 305 LMLV 305

[Close]

BLAST of Cucsat.G20977.T4 vs. NCBI nr
Match: KAG7024285.1 (Isoflavone 2'-hydroxylase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 493 bits (1270), Expect = 4.66e-171
Identity = 254/304 (83.55%), Postives = 269/304 (88.49%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           M I FL LLIL +L IFTN FLHRIRNLPPSPF SLPIIGQLYLLK PLHRTLS+ISRRH
Sbjct: 1   MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPI+FF FGFR VLVVSSPSAAE+CLSKND++FANRP L+ GKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEI S+HRLQTLSSIRVDEVRTLIRRLYN +NDVVDMRTEFFE+MFNI MR
Sbjct: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMRTEFFELMFNIMMR 180

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGED AA SEEA RFREIQEETFRLS KTNLGDFLR+VKW GLSKG+ENR++E
Sbjct: 181 MIAGKRYYGED-AAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKE 240

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300
           LQ KRD WMQSLIEEHRK+ N           DAKKTMIEVLLSLQQKEP YYKDEYIRG
Sbjct: 241 LQSKRDAWMQSLIEEHRKQSNK----------DAKKTMIEVLLSLQQKEPHYYKDEYIRG 293

Query: 301 LMLV 304
           LMLV
Sbjct: 301 LMLV 293

[Close]

BLAST of Cucsat.G20977.T4 vs. NCBI nr
Match: XP_023535659.1 (isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 493 bits (1270), Expect = 4.66e-171
Identity = 253/304 (83.22%), Postives = 269/304 (88.49%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           M I FL LLIL +L IFTN FLHRIRNLPPSPF SLPIIGQLYLLK PLHRTLS+ISRRH
Sbjct: 1   MAITFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPI+FF FGFR VLVVSSPSAAE+CLSKND++FANRP L+ GKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRR+SSLEI S+HRLQTLSSIRVDEVRTLIRRLYN ENDVVDMRTEFFE+MFNI MR
Sbjct: 121 WRNLRRVSSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTENDVVDMRTEFFELMFNIMMR 180

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGED AA SEEA RFREIQEETFRLS KTNLGDFLR+VKW GLSKG+ENR++E
Sbjct: 181 MIAGKRYYGED-AAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKE 240

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300
           LQ KRD WMQSLIEEHRK+             DAKKTMIEVLLSLQQKEP+YYKDEYIRG
Sbjct: 241 LQSKRDAWMQSLIEEHRKQSKK----------DAKKTMIEVLLSLQQKEPRYYKDEYIRG 293

Query: 301 LMLV 304
           LMLV
Sbjct: 301 LMLV 293

[Close]

BLAST of Cucsat.G20977.T4 vs. ExPASy TrEMBL
Match: A0A0A0L0F1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G642290 PE=3 SV=1)

HSP 1 Score: 595 bits (1535), Expect = 1.63e-211
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH
Sbjct: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR
Sbjct: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE
Sbjct: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300
           LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG
Sbjct: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300

Query: 301 LMLV 304
           LMLV
Sbjct: 301 LMLV 304

[Close]

BLAST of Cucsat.G20977.T4 vs. ExPASy TrEMBL
Match: A0A1S3BVF3 (cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103493585 PE=3 SV=1)

HSP 1 Score: 544 bits (1402), Expect = 7.93e-191
Identity = 282/305 (92.46%), Postives = 290/305 (95.08%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           MVI FL+LLILF+LQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLK PLHRTLSEISRRH
Sbjct: 31  MVISFLYLLILFILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRH 90

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPI+FFQFGFR VLVVSSPSA EDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH
Sbjct: 91  GPILFFQFGFRPVLVVSSPSAVEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 150

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEILSSHRLQTLSSIRVDEVR LIRRLYNAENDVVDMRTEFFE+MFNI MR
Sbjct: 151 WRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAENDVVDMRTEFFELMFNIMMR 210

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGE V A SEE  RF+EIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE
Sbjct: 211 MIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 270

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSS-IQTDAKKTMIEVLLSLQQKEPQYYKDEYIR 300
           LQI+RDDWMQSLIEEHRKKM+NA+SSSSS IQ DAKKTMIEVLLSLQQ EPQ YKDEYIR
Sbjct: 271 LQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQNYKDEYIR 330

Query: 301 GLMLV 304
           GLMLV
Sbjct: 331 GLMLV 335

[Close]

BLAST of Cucsat.G20977.T4 vs. ExPASy TrEMBL
Match: A0A6J1FED9 (isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111443311 PE=3 SV=1)

HSP 1 Score: 491 bits (1264), Expect = 1.84e-170
Identity = 253/304 (83.22%), Postives = 268/304 (88.16%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           M I FL LLIL +L IFTN FLHRIRNLPPSPF SLPIIGQLYLLK PLHRTLS+ISRRH
Sbjct: 1   MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPI+FF FGFR VLVVSSPSAAE+CLSKND++FANRP L+ GKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEI S+HRLQTLSSIRVDEVRTLIRRLYN +NDVVDMRTEFFE+MFNI MR
Sbjct: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMRTEFFELMFNIMMR 180

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGED AA SEEA RFREIQEETFRLS KTNLGDFLR+VKW GLSKG+ENR++E
Sbjct: 181 MIAGKRYYGED-AAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKE 240

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300
           LQ KRD WMQSLIEEHRK+             DAKKTMIEVLLSLQQKEP YYKDEYIRG
Sbjct: 241 LQSKRDAWMQSLIEEHRKQSKK----------DAKKTMIEVLLSLQQKEPHYYKDEYIRG 293

Query: 301 LMLV 304
           LMLV
Sbjct: 301 LMLV 293

[Close]

BLAST of Cucsat.G20977.T4 vs. ExPASy TrEMBL
Match: A0A6J1INS3 (isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111477207 PE=3 SV=1)

HSP 1 Score: 490 bits (1262), Expect = 3.70e-170
Identity = 252/304 (82.89%), Postives = 268/304 (88.16%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           M I FL LLILF+L IFTN FLHRIRNLPPSPF SLPIIGQLYLLK PLHRTL +ISRRH
Sbjct: 1   MAITFLCLLILFILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLLDISRRH 60

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPI+FF FGFR VLVVSSPSAAE+CLSKND++FANRP L+ GKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEI S+HRLQTLSSIRVDEVRTLIRRLYN +NDVVDM+TEFFE+MFNI MR
Sbjct: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMKTEFFELMFNIMMR 180

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGEDVA  SEEA RFREIQEETFRLS KTNLGDFLR+VKW GLSKG+ENR++E
Sbjct: 181 MIAGKRYYGEDVAE-SEEAERFREIQEETFRLSSKTNLGDFLRMVKWAGLSKGLENRMKE 240

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300
           LQ KRD WMQSLIEEHRK+             DAKKTMIEVLLSLQQKEP YYKDEYIRG
Sbjct: 241 LQSKRDAWMQSLIEEHRKQSKK----------DAKKTMIEVLLSLQQKEPHYYKDEYIRG 293

Query: 301 LMLV 304
           LMLV
Sbjct: 301 LMLV 293

[Close]

BLAST of Cucsat.G20977.T4 vs. ExPASy TrEMBL
Match: A0A6P4BCT8 (cytochrome P450 81E8-like OS=Ziziphus jujuba OX=326968 GN=LOC107433678 PE=3 SV=1)

HSP 1 Score: 379 bits (973), Expect = 4.36e-126
Identity = 194/306 (63.40%), Postives = 240/306 (78.43%), Query Frame = 0


Query: 5   FLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRHGPIV 64
           F ++L+   + +FT H  H+I+NLPPSPF  LP+IG LYLL+ PLHRTLS+++ R+GPI+
Sbjct: 6   FFYILLFSAVYVFTKHLFHKIQNLPPSPF-PLPVIGHLYLLERPLHRTLSKLAGRYGPIL 65

Query: 65  FFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNL 124
           F + G R VLVVSS SAAE+CL+KND+IFANRPRL+ GKYLGYN+TS+ WAPYG+HWRNL
Sbjct: 66  FLKLGSRPVLVVSSSSAAEECLAKNDIIFANRPRLMVGKYLGYNFTSVAWAPYGDHWRNL 125

Query: 125 RRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMRMIAG 184
           RRISSLE+LSSHRLQ LS IR++EV+ L+RRL+  +  VVDMR  FFE+  NI MRMIAG
Sbjct: 126 RRISSLELLSSHRLQMLSGIRLEEVKLLLRRLFEKQGVVVDMRQAFFELTLNIMMRMIAG 185

Query: 185 KRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRELQIK 244
           KRYYG++V    +EA RFREIQEET RLSGK+NLGDFL  V+W+G SK +E  L ELQ K
Sbjct: 186 KRYYGDEVVEDLKEAMRFREIQEETVRLSGKSNLGDFLPFVRWIGGSKRLEKSLMELQRK 245

Query: 245 RDDWMQSLIEEHRKKMNN------AYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYI 304
           RD ++Q LIEEHR+  +       + S S S   + +KTM+EVLLSLQQ EP+YYKDE I
Sbjct: 246 RDGFLQDLIEEHRRTASRIANDCCSPSPSPSKSKERRKTMLEVLLSLQQMEPEYYKDEII 305

The following BLAST results are available for this feature:

BLAST of Cucsat.G20977.T4 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
W8JMU7	2.9e-64	43.83	Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1	[more]
Q6WNQ8	8.0e-62	45.31	Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1	[more]
P93147	1.7e-59	43.19	Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2	[more]
Q6WNR0	3.5e-57	41.20	Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1	[more]
Q6WNQ9	4.6e-57	43.00	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P...	[more]

BLAST of Cucsat.G20977.T4 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004141396.1	3.36e-211	100.00	cytochrome P450 81Q32 [Cucumis sativus] >XP_011654142.1 cytochrome P450 81Q32 [C...	[more]
XP_008452618.1	1.64e-190	92.46	PREDICTED: cytochrome P450 81E8-like [Cucumis melo]	[more]
XP_038899302.1	2.11e-186	88.16	cytochrome P450 81Q32-like [Benincasa hispida]	[more]
KAG7024285.1	4.66e-171	83.55	Isoflavone 2'-hydroxylase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023535659.1	4.66e-171	83.22	isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cucsat.G20977.T4 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L0F1	1.63e-211	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G642290 PE=3 SV=1	[more]
A0A1S3BVF3	7.93e-191	92.46	cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103493585 PE=3 SV=1	[more]
A0A6J1FED9	1.84e-170	83.22	isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111443311 PE=...	[more]
A0A6J1INS3	3.70e-170	82.89	isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111477207 PE=3 ...	[more]
A0A6P4BCT8	4.36e-126	63.40	cytochrome P450 81E8-like OS=Ziziphus jujuba OX=326968 GN=LOC107433678 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 173..191 score: 24.18 coord: 57..76 score: 28.43 coord: 81..102 score: 25.45
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 23..304 e-value: 1.3E-46 score: 161.2
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 29..303
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 30..299 e-value: 2.7E-23 score: 82.4
None	No IPR available	PANTHER	PTHR47947	CYTOCHROME P450 82C3-RELATED	coord: 13..304
None	No IPR available	PANTHER	PTHR47947:SF24	CYTOCHROME P450 81E8	coord: 13..304

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G20977	Cucsat.G20977	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G20977.T4.E1	Cucsat.G20977.T4.E1	exon
Cucsat.G20977.T4.E2	Cucsat.G20977.T4.E2	exon
Cucsat.G20977.T4.E3	Cucsat.G20977.T4.E3	exon
Cucsat.G20977.T4.E4	Cucsat.G20977.T4.E4	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G20977.T4.C1	Cucsat.G20977.T4.C1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G20977.T4	Cucsat.G20977.T4-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0098542	defense response to other organism
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen