Cucsat.G20977.T7 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G20977.T7
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	cytochrome P450 81E8-like
Location	ctg907: 474622 .. 477394 (+)
RNA-Seq Expression	Cucsat.G20977.T7
Synteny	Cucsat.G20977.T7

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCTTTCAGGATATTTAAGGAAACTTGAAAGGAATTTGTGTATAAGATTTTTTCTGGCTCTTCTTCGTGACTTGAGGTTTTATGGGTTTTTGGTTTTCACCCAACCAAGAAAGCTAATGTTTCTTCTTCGGATGGAAGATAGTTCATGGATGGTAACGTTTTTCTTCTTTAATTCATTTAACATTTTAGTTTTGAAATTTTCAGTATTGATACTGTTTTTGAGATTTTGTTGTAGAGGACATTCAGTTTACAATGAATAATGTAAATATTATATTCTTTGTTCATATATCTAAGTTTGTCTTCCAGGATTCTCTTTCACTAAAGATTCATTTTTTTCATTAATTATTTAGTTTCTATGAAATCTGAATCAATTACAAGGAATCACAGAAGCGATTACTCACGAGGTCAGTTTATCTAATTTTAATTTACGGAGATCCATATAACTTTTTTTTTCTTTCAATTATGGATTTCAATTTTGTTCATTTTCTTTCTACATTTTTTTTTGTCAATAATTTACTCTTTTACAACTTGTATATAATTGGCCGTATATTTCACCATACAAATTATATACAATTATATATCCATTATTTCTTTCTTCTTGTTTTAAAATAATATAACTTTCTATTAATTTTTTTATAGTTTAAACAAAATGTTAGATCACATAAATTTAGTTTTTTTAATTTCCATCAAACCCATTTTTTAGTTTAAACTTAGAGGTAAAAACAAATTTAATAGAATGTATAACACTCATCTATATTTAAGTATTATCTAAGTTTTTGAGTGATGATAAATTTAATATAACATCCAGTGTAACTAAATTGCCGAATTGACTTGCAGGTAAGAAAGCAACTTGAAAAATTTAAACGGAACTCAATGATACTTCGACCAACCCAACTAGCATGGTTATAATTTACAAATGATTCTCGCAGGTAAATTTCATGGTGATCCCATTCCTACATCTTCTGATTTTATTCTTACTTCAAATCTTCACAAATCACTTCCTTCATAGAATCCGAAATCTCCCTCCCAGCCCATTTCTTTCTCTACCCATTATAGGCCAACTATACCTCCTTAAAACACCCCTCCACCGGACCTTATCGGAGATCTCCCGCCGTCATGGCCCAATCGTCTTCTTCCAATTCGGTTTCCGTTCAGTCCTGGTCGTTTCATCTCCGTCGGCCGCCGAGGACTGCCTATCCAAAAACGACGTCATCTTCGCCAACCGACCTCGTCTCGTTAGCGGCAAATACCTCGGGTACAATTACACAAGCCTCCTGTGGGCACCGTACGGCGAGCATTGGCGAAACCTCCGTCGAATCTCGTCGTTAGAAATCCTTTCCAGCCACCGCCTCCAAACGCTTTCAAGTATTCGCGTGGATGAAGTCCGTACGCTGATCCGGAGACTGTACAACGCTGAAAACGACGTCGTAGACATGAGAACAGAATTTTTCGAAGTGATGTTCAATATCACGATGAGAATGATCGCCGGAAAACGATATTACGGGGAAGATGTGGCAGCGTACTCGGAGGAGGCGGGGAGATTTCGAGAGATACAAGAGGAGACGTTTCGATTAAGCGGCAAGACTAATTTGGGGGACTTTTTAAGGGTTGTGAAATGGGTTGGATTATCTAAAGGGATTGAAAATAGATTGAGGGAGTTACAGATTAAGAGAGATGATTGGATGCAGAGTTTGATTGAAGAACATCGGAAGAAAATGAATAATGCTTATAGTTCTTCTTCTTCTATTCAAACTGATGCTAAAAAGACTATGATTGAGGTTCTCCTCTCATTGCAACAAAAGGAACCTCAGTATTATAAGGACGAGTATATCAGAGGCCTCATGCTCGTGAGTTAACTTGATTTCTCTCTTAGTTATTCAAAAGACATAACCAAATAGATTTTGAAACACTATTTTAAACACATATGAAAATATATGTAATCCGACTCTATTTTTCCTTAAATCAACCAAATCGTCCCAAATGAAATTGGATTGTTGTAGGTGTTGCTATTAGCTGGAACGGAAGGTTCCATCAATACAATGGAGTGGCTTCTATCACTATTACTCAACCACCCACACTCTCTTCAACGAGCCCAAATGGAAATCGACGACGTAGTTGGAAGAACTAATCGGCTCCTGGAGGAATCAGATCTAACCCACCTTCCTTACCTCCGTAGCCTGATCCACGAAACTCTTCGAATGTACCCGCCAGGCCCATTACTGATTCCACACGAATCGTCAGAGGATTGCCACGTTGGAGGGTTCCATGTCCCAGCTGGCACAATGTTATTCGTCAACGTATGGGCCATTCAAAACGACCCCACCGTATGGGTGGAGCCGAGGAAATTTAACCCAGATAGATTCGGAGGGGATGGAGAGGGGTTCAAATGGATGCCATTTGGGGCTGGGCGGAGGAGGTGTCCCGGCGAGGGCTTGGGGCTTCGCGTCATTGGATTGGTTGTAGGGTCGCTGATTCAATGCTTTGAATGGGAGTCAATGGACGGAGAATGTATTGATATGAGCGAAGGTGGTGGATTGACCTTGCCGAAAGCTCTCCCCCTTCGCACCCTTTGCCGGCCACGTTCAAATGCCACACATTTGCTTTCCCAAATCTAATTCAAAATGCATGGTCTTGTTTATGTTGTTCAACAACAATTAATTCTGTACATAAATTTTACATATTTTAGTAGTAGTTGAAAACACCTACGTTATTTCCAATTTGTTGACGTCCTATTTTTCTTTTTTCTTTTGCCTCTTTGTTTACGTGATGATTG

Coding sequence (CDS)

ATGGTGATCCCATTCCTACATCTTCTGATTTTATTCTTACTTCAAATCTTCACAAATCACTTCCTTCATAGAATCCGAAATCTCCCTCCCAGCCCATTTCTTTCTCTACCCATTATAGGCCAACTATACCTCCTTAAAACACCCCTCCACCGGACCTTATCGGAGATCTCCCGCCGTCATGGCCCAATCGTCTTCTTCCAATTCGGTTTCCGTTCAGTCCTGGTCGTTTCATCTCCGTCGGCCGCCGAGGACTGCCTATCCAAAAACGACGTCATCTTCGCCAACCGACCTCGTCTCGTTAGCGGCAAATACCTCGGGTACAATTACACAAGCCTCCTGTGGGCACCGTACGGCGAGCATTGGCGAAACCTCCGTCGAATCTCGTCGTTAGAAATCCTTTCCAGCCACCGCCTCCAAACGCTTTCAAGTATTCGCGTGGATGAAGTCCGTACGCTGATCCGGAGACTGTACAACGCTGAAAACGACGTCGTAGACATGAGAACAGAATTTTTCGAAGTGATGTTCAATATCACGATGAGAATGATCGCCGGAAAACGATATTACGGGGAAGATGTGGCAGCGTACTCGGAGGAGGCGGGGAGATTTCGAGAGATACAAGAGGAGACGTTTCGATTAAGCGGCAAGACTAATTTGGGGGACTTTTTAAGGGTTGTGAAATGGGTTGGATTATCTAAAGGGATTGAAAATAGATTGAGGGAGTTACAGATTAAGAGAGATGATTGGATGCAGAGTTTGATTGAAGAACATCGGAAGAAAATGAATAATGCTTATAGTTCTTCTTCTTCTATTCAAACTGATGCTAAAAAGACTATGATTGAGGTTCTCCTCTCATTGCAACAAAAGGAACCTCAGTATTATAAGGACGAGTATATCAGAGGCCTCATGCTCGTGTTGCTATTAGCTGGAACGGAAGGTTCCATCAATACAATGGAGTGGCTTCTATCACTATTACTCAACCACCCACACTCTCTTCAACGAGCCCAAATGGAAATCGACGACGTAGTTGGAAGAACTAATCGGCTCCTGGAGGAATCAGATCTAACCCACCTTCCTTACCTCCGTAGCCTGATCCACGAAACTCTTCGAATGTACCCGCCAGGCCCATTACTGATTCCACACGAATCGTCAGAGGATTGCCACGTTGGAGGGTTCCATGTCCCAGCTGGCACAATGTTATTCGTCAACGTATGGGCCATTCAAAACGACCCCACCGTATGGGTGGAGCCGAGGAAATTTAACCCAGATAGATTCGGAGGGGATGGAGAGGGGTTCAAATGGATGCCATTTGGGGCTGGGCGGAGGAGGTGTCCCGGCGAGGGCTTGGGGCTTCGCGTCATTGGATTGGTTGTAGGGTCGCTGATTCAATGCTTTGAATGGGAGTCAATGGACGGAGAATGTATTGATATGAGCGAAGGTGGTGGATTGACCTTGCCGAAAGCTCTCCCCCTTCGCACCCTTTGCCGGCCACGTTCAAATGCCACACATTTGCTTTCCCAAATCTAA

Protein sequence

MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRHGPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMRMIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRELQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYLRSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFNPDRFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEGGGLTLPKALPLRTLCRPRSNATHLLSQI

Homology

[Close]

BLAST of Cucsat.G20977.T7 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 3.4e-134
Identity = 248/511 (48.53%), Postives = 351/511 (68.69%), Query Frame = 0


Query: 1   MVIPFLHLLILFL-LQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRR 60
           ++  FL +++L + L++F      R RNLPPSP L+LP+IG L+L+   LHR+L ++S++
Sbjct: 6   LLYTFLAVVLLSISLKLFP--VSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKK 65

Query: 61  HGPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGE 120
           +G +   Q G R VLVVSSP+AAE+C +KND++FANRP  + GKY+GYNYT+++ +PYGE
Sbjct: 66  YGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGE 125

Query: 121 HWRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAEND---VVDMRTEFFEVMFN 180
           HWRNLRR++++EI S+  L    SIR DEV+ L+  LY +       V+M+++  E+ FN
Sbjct: 126 HWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFN 185

Query: 181 ITMRMIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIEN 240
           +TMRM+AGKRY+G+DV   S+EA  FR +  E F  +G +N GDF+  ++W+   K  E 
Sbjct: 186 VTMRMVAGKRYFGQDVD--SDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDF-KNYEK 245

Query: 241 RLRELQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDE 300
           ++ ++  + D ++Q LI E R   NN              TMI+ LLSLQ+ +P+YY D+
Sbjct: 246 KVSKISQEMDAFLQRLIHESRINKNNV-------------TMIDHLLSLQESQPEYYTDQ 305

Query: 301 YIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTH 360
            I+G+++VLLLAGT+ S  T+EW +SLLLNHP +L++A+ EI+  VG +NRL+EE DL  
Sbjct: 306 IIKGIIMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVG-SNRLIEEQDLPK 365

Query: 361 LPYLRSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEP 420
           L YL ++I ET R+ P  P+L+PHESS+DC V G+ VP GT+L VN WAI  DP  W EP
Sbjct: 366 LTYLHNIISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEP 425

Query: 421 RKFNPDRFGG-DGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECID 480
             F P+R GG + E  K MPFG GRR CPG GL  RV+GL +G+LIQCFEW+ +    ID
Sbjct: 426 TLFKPERHGGVELEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKID 485

Query: 481 MSEGGGLTLPKALPLRTLCRPRSNATHLLSQ 507
           M+EG GLT+PKA PL  LC+PR+    ++S+
Sbjct: 486 MAEGSGLTMPKAQPLEALCKPRNILHKVVSE 497

[Close]

BLAST of Cucsat.G20977.T7 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 440.3 bits (1131), Expect = 3.0e-122
Identity = 224/495 (45.25%), Postives = 329/495 (66.46%), Query Frame = 0


Query: 7   HLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRHGPIVFF 66
           +L + F+  I       + +NLPP P  SLPIIG L+ LK PLHRT   +S ++G +   
Sbjct: 13  YLALFFIFNIVIR--ARKFKNLPPGP-PSLPIIGNLHHLKRPLHRTFKGLSEKYGHVFSL 72

Query: 67  QFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLRR 126
            FG R V+VVSS S  + C +KNDV+ ANRPR +SGKY+ YNYT+L    YGEHWRNLRR
Sbjct: 73  WFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRR 132

Query: 127 ISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAEND---VVDMRTEFFEVMFNITMRMIA 186
           I++L++LS+HR+ + S IR DE + LI RL +  +     +++ +  +++ FN  MRMI+
Sbjct: 133 ITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMRMIS 192

Query: 187 GKRYYGEDV-AAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRELQ 246
           GKRYYGED   +  +EA +FR++  E  +LSG  N  DF+ +++++   + +E RL+++ 
Sbjct: 193 GKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDF-ENLEKRLKDIS 252

Query: 247 IKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRGLM 306
            K D +++ LIEEHR K   A             TMI+ LL+LQ  +P+YY D+ I+GL 
Sbjct: 253 GKTDAFLRGLIEEHRTKKERA------------NTMIDHLLNLQDSQPEYYTDQIIKGLA 312

Query: 307 LVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYLRS 366
           L +LLAGT+ S  T+EW +S LLNHP  L++ + E+D  VG+ +RL++ESDL  L YL++
Sbjct: 313 LAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQ-DRLVDESDLPKLTYLKN 372

Query: 367 LIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFNPD 426
           +I+ETLR+Y P PLL+PH +S++C++GG+ VP  T++ +N WAI  DP +W E   F P+
Sbjct: 373 VINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPE 432

Query: 427 RFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEGGGL 486
           RF   GE  K + FG GRR CPGEGL +R I + +  LIQCF+W+ ++G+ ID++E  G 
Sbjct: 433 RFEKKGELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGF 490

Query: 487 TLPKALPLRTLCRPR 498
           TL K +PL+ +C+ R
Sbjct: 493 TLTKLVPLKAMCKSR 490

[Close]

BLAST of Cucsat.G20977.T7 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 1.7e-120
Identity = 232/502 (46.22%), Postives = 326/502 (64.94%), Query Frame = 0


Query: 5   FLHLLI-LFLLQIFTNHFLH---RIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 64
           +L L+I LF L I    F +   + +NLPP P   LPIIG L+ LK PLH T   +S+++
Sbjct: 5   YLSLIISLFFLIITLKVFFNTSRKFKNLPPGP-QCLPIIGNLHQLKQPLHHTFHTLSQKY 64

Query: 65  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 124
           G I    FG R V+VVSS + A++C +KND++ ANRP  ++GKY+GYN T++  +PYG+H
Sbjct: 65  GQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDH 124

Query: 125 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRL----YNAENDVVDMRTEFFEVMFN 184
           WRNLRRI S+EILSSHRL +   IR DE+  LI++L    YN   + V++R  F E+ FN
Sbjct: 125 WRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTE-VELRPMFSEMTFN 184

Query: 185 ITMRMIAGKRYYGEDV-AAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIE 244
             MRM++GKRYYG D   +  EEA  FR I +E   L G  N+GDFL  ++W     G+E
Sbjct: 185 TIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFD-GLE 244

Query: 245 NRLRELQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKD 304
            RL+++  + D ++Q LI+EHR    N+             TMI+ LL+ QQ +P+YY D
Sbjct: 245 KRLKKISKRTDAFLQGLIDEHRFGKRNS------------NTMIDHLLTQQQSQPEYYTD 304

Query: 305 EYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLT 364
           + I+GLM+V+LLAGT+ S  T+EW +S LLNHP  +++A+ E+D  +G  +R ++E D++
Sbjct: 305 QIIKGLMVVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGH-DRQVDEHDIS 364

Query: 365 HLPYLRSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVE 424
            LPYL+S+++ETLR++   PLL+PH SSED  +GG+++P  T+L VN W I  DP +W +
Sbjct: 365 KLPYLQSIVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSD 424

Query: 425 PRKFNPDRFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECID 484
           P  F P+RF  +GE  K + FG GRR CPGE L  R  GL +G LIQCFEW+ +  E ID
Sbjct: 425 PTCFKPERFEKEGEVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKID 484

Query: 485 MSEGGGLTLPKALPLRTLCRPR 498
           M E  G+T  K   L  +C+ R
Sbjct: 485 MVEAKGITAGKKTSLNAMCKVR 490

[Close]

BLAST of Cucsat.G20977.T7 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 6.3e-120
Identity = 230/500 (46.00%), Postives = 323/500 (64.60%), Query Frame = 0


Query: 6   LHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRHGPIVF 65
           L L  +  ++I       R++NLPP P  ++PIIG L+ LK PLHRT + +S+ +G I  
Sbjct: 8   LSLSFIITIKILLKITSRRLKNLPPGP-PTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFS 67

Query: 66  FQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLR 125
             FG R V+VVSSPS A +C +KND+I ANRPR ++GKY+ YNYT+L  A YG+HWRNLR
Sbjct: 68  LWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLR 127

Query: 126 RISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDV---------VDMRTEFFEVMFN 185
           RI+++++LS++RL +   +R DE   LI++L     DV         V++R    E+ FN
Sbjct: 128 RITTIDVLSNNRLNSFLGVRRDETNRLIQKLL---KDVVSEGFGFTKVELRPRLTEMTFN 187

Query: 186 ITMRMIAGKRYYGED-VAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIE 245
             MRMI+GKRYYG+D   +  EEA +FREI  E   L G  N GDFL +++ V L   +E
Sbjct: 188 AMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLD-NLE 247

Query: 246 NRLRELQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKD 305
            R + +  + + +++ LIEEHR+   N +S           TMI+ LL L + +P+YY D
Sbjct: 248 KRCKRIAKRSNAFLEGLIEEHRR--GNIHSDGG--------TMIDHLLKLSESQPEYYSD 307

Query: 306 EYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLT 365
             I+GL+  +LLAGT+ S  T+EW++S LLNHP  L++A+ E+D  +G+ N+L++E DL+
Sbjct: 308 HLIKGLIQGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGK-NKLVDEQDLS 367

Query: 366 HLPYLRSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVE 425
            LPYL+++I ETLR++PP PLL+PH SSEDC +G F+VP  T++  NVW I  DP  W +
Sbjct: 368 KLPYLQNIISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWND 427

Query: 426 PRKFNPDRFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECID 485
              F P+RF  + E  K M FG GRR CPG  L  R +G  VG LIQCFEWE    E +D
Sbjct: 428 ALSFKPERFEKEEEVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLD 487

Query: 486 MSEGGGLTLPKALPLRTLCR 496
           M EG G+T+P  +PLR +C+
Sbjct: 488 MMEGKGITMPMKIPLRAMCK 491

[Close]

BLAST of Cucsat.G20977.T7 vs. ExPASy Swiss-Prot
Match: Q0WTF4 (Cytochrome P450 81F3 OS=Arabidopsis thaliana OX=3702 GN=CYP81F3 PE=2 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 2.4e-119
Identity = 228/498 (45.78%), Postives = 321/498 (64.46%), Query Frame = 0


Query: 9   LILFLL--QIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRHGPIVFF 68
           L LFLL  ++F      R  NLPPSP  SLPI+G   LLK P+HR    +S+ HGPI   
Sbjct: 10  LALFLLAYKLFFTSKTKRF-NLPPSPPYSLPILGHHNLLKPPVHRLFHRLSKTHGPIFSL 69

Query: 69  QFGFRSVLVVSSPSAAEDCLS-KNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLR 128
           QFG R  +V+SS S A  C + +ND+I +NRP  ++ KY+ YNYT++  APYG+HWRNLR
Sbjct: 70  QFGSRRAVVISSSSLATQCFTGQNDIILSNRPCFLTAKYVAYNYTTVGTAPYGDHWRNLR 129

Query: 129 RISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMRMIAGK 188
           RI SLEILSS+RL     IR DE+  ++ RL    N  +++     ++ FN  +RM+ GK
Sbjct: 130 RICSLEILSSNRLTNFLHIRKDEIHRMLTRLSRDVNKEIELEPLLSDLTFNNIVRMVTGK 189

Query: 189 RYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRELQIKR 248
           RYYG++V    EEA  F+++  +    SG  + GD+L  +K  G S   E +++ L    
Sbjct: 190 RYYGDEV-HNEEEANVFKKLVADINDCSGARHPGDYLPFMKMFGGS--FEKKVKALAEAM 249

Query: 249 DDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRGLMLVL 308
           D+ +Q L+EE ++  +               TM+  LLSLQQ EP+YY D  I+GLML +
Sbjct: 250 DEILQRLLEECKRDKDG-------------NTMVNHLLSLQQNEPEYYTDVTIKGLMLGM 309

Query: 309 LLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYLRSLIH 368
           ++AGT+ S  T+EW +S LLNHP +L++A++EID+ +G+  RL++E D+ +LPYL++++ 
Sbjct: 310 MIAGTDTSAVTLEWAMSSLLNHPEALEKAKLEIDEKIGQ-ERLIDEPDIANLPYLQNIVS 369

Query: 369 ETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFNPDRF- 428
           ET R+YP  PLL+P   +ED  VGG+ VP GTM+ VN WAI  DP +W EP KF P+RF 
Sbjct: 370 ETFRLYPAAPLLVPRSPTEDIKVGGYDVPRGTMVMVNAWAIHRDPELWNEPEKFKPERFN 429

Query: 429 GGDGEG-----FKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEG 488
           GG+G G      K MPFG GRR CPG GLG +++ L +GSLIQCF+W+ ++GE IDM+E 
Sbjct: 430 GGEGGGRGEDVHKLMPFGNGRRSCPGAGLGQKIVTLALGSLIQCFDWQKVNGEAIDMTET 489

Query: 489 GGLTLPKALPLRTLCRPR 498
            G+ + K +PL  LC+ R
Sbjct: 490 PGMAMRKKIPLSALCQSR 489

[Close]

BLAST of Cucsat.G20977.T7 vs. NCBI nr
Match: XP_004141396.1 (cytochrome P450 81Q32 [Cucumis sativus] >XP_011654142.1 cytochrome P450 81Q32 [Cucumis sativus] >XP_031739532.1 cytochrome P450 81Q32 [Cucumis sativus] >KGN55248.1 hypothetical protein Csa_012192 [Cucumis sativus])

HSP 1 Score: 1019 bits (2636), Expect = 0.0
Identity = 507/507 (100.00%), Postives = 507/507 (100.00%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH
Sbjct: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR
Sbjct: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE
Sbjct: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300
           LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG
Sbjct: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300

Query: 301 LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL 360
           LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL
Sbjct: 301 LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL 360

Query: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN 420
           RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN
Sbjct: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN 420

Query: 421 PDRFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEGG 480
           PDRFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEGG
Sbjct: 421 PDRFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEGG 480

Query: 481 GLTLPKALPLRTLCRPRSNATHLLSQI 507
           GLTLPKALPLRTLCRPRSNATHLLSQI
Sbjct: 481 GLTLPKALPLRTLCRPRSNATHLLSQI 507

[Close]

BLAST of Cucsat.G20977.T7 vs. NCBI nr
Match: XP_008452618.1 (PREDICTED: cytochrome P450 81E8-like [Cucumis melo])

HSP 1 Score: 948 bits (2450), Expect = 0.0
Identity = 473/508 (93.11%), Postives = 488/508 (96.06%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           MVI FL+LLILF+LQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLK PLHRTLSEISRRH
Sbjct: 31  MVISFLYLLILFILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRH 90

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPI+FFQFGFR VLVVSSPSA EDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH
Sbjct: 91  GPILFFQFGFRPVLVVSSPSAVEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 150

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEILSSHRLQTLSSIRVDEVR LIRRLYNAENDVVDMRTEFFE+MFNI MR
Sbjct: 151 WRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAENDVVDMRTEFFELMFNIMMR 210

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGE V A SEE  RF+EIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE
Sbjct: 211 MIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 270

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSS-IQTDAKKTMIEVLLSLQQKEPQYYKDEYIR 300
           LQI+RDDWMQSLIEEHRKKM+NA+SSSSS IQ DAKKTMIEVLLSLQQ EPQ YKDEYIR
Sbjct: 271 LQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQNYKDEYIR 330

Query: 301 GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPY 360
           GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSL+RAQMEID+VVG+TNRLLEESDLTHLPY
Sbjct: 331 GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDLTHLPY 390

Query: 361 LRSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKF 420
           LRSLIHETLRMYPPGPLLIPHESS+DCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEP KF
Sbjct: 391 LRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPEKF 450

Query: 421 NPDRFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEG 480
           NPDRF  DGEGFKWMPFGAGRRRCPGE LGLRV+GLV+GSLIQCFEWESM G+CIDMSEG
Sbjct: 451 NPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCIDMSEG 510

Query: 481 GGLTLPKALPLRTLCRPRSNATHLLSQI 507
           GGLTLPKALPLRTLCRPRSNATHLLSQI
Sbjct: 511 GGLTLPKALPLRTLCRPRSNATHLLSQI 538

[Close]

BLAST of Cucsat.G20977.T7 vs. NCBI nr
Match: XP_038899302.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 914 bits (2362), Expect = 0.0
Identity = 448/507 (88.36%), Postives = 476/507 (93.89%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           M IPFL+LL+LF+LQIFTNHFLHRIRNLPPSP+LSLPIIGQLYLLK+PLHRTLSEI+ RH
Sbjct: 5   MAIPFLYLLVLFILQIFTNHFLHRIRNLPPSPYLSLPIIGQLYLLKSPLHRTLSEIAHRH 64

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPI+FF+FGFR VLVVSSPSAAEDCLSKNDV+FANRPRL SGKYLGYNYTSLLWAPYGEH
Sbjct: 65  GPILFFRFGFRQVLVVSSPSAAEDCLSKNDVVFANRPRLFSGKYLGYNYTSLLWAPYGEH 124

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEILS HRLQTLSSIRVDEVRTLIRRLYNA+NDVVDMRTEFFE+MFNI MR
Sbjct: 125 WRNLRRISSLEILSKHRLQTLSSIRVDEVRTLIRRLYNAKNDVVDMRTEFFELMFNIMMR 184

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGEDVAA +EE GRFREIQEETFRLS KTNLGDFLRVVKWVGLSKG+EN+LRE
Sbjct: 185 MIAGKRYYGEDVAADTEEVGRFREIQEETFRLSSKTNLGDFLRVVKWVGLSKGLENKLRE 244

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300
           LQ+KRD WMQSLIEEHRK+MNN   +S+S+Q DAKKTMIEVLLSLQQ EP YYKDE+IRG
Sbjct: 245 LQMKRDVWMQSLIEEHRKQMNN---TSTSLQNDAKKTMIEVLLSLQQMEPHYYKDEFIRG 304

Query: 301 LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL 360
           LMLVLLLAGTEGSINTMEWLLSLLLNHP  LQRAQMEIDDVVGR+NR+LEESDL HLPYL
Sbjct: 305 LMLVLLLAGTEGSINTMEWLLSLLLNHPDCLQRAQMEIDDVVGRSNRILEESDLAHLPYL 364

Query: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN 420
           RSLIHETLRMYPPGPLLIPHESSEDCHVGGF VPAGTMLFVNVWAIQNDPTVWVEP  FN
Sbjct: 365 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFCVPAGTMLFVNVWAIQNDPTVWVEPGNFN 424

Query: 421 PDRFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEGG 480
           P+RFG +GEGFKWMPFGAGRRRCPGEGLGLRV+GL VGSLIQCF+WES+ GEC+DM EGG
Sbjct: 425 PERFGSEGEGFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFDWESVGGECVDMREGG 484

Query: 481 GLTLPKALPLRTLCRPRSNATHLLSQI 507
           GLTLPKALPL  LCRPRSN  HLL QI
Sbjct: 485 GLTLPKALPLHALCRPRSNVAHLLPQI 508

[Close]

BLAST of Cucsat.G20977.T7 vs. NCBI nr
Match: KAG7024285.1 (Isoflavone 2'-hydroxylase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 860 bits (2222), Expect = 2.03e-312
Identity = 431/509 (84.68%), Postives = 453/509 (89.00%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           M I FL LLIL +L IFTN FLHRIRNLPPSPF SLPIIGQLYLLK PLHRTLS+ISRRH
Sbjct: 1   MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPI+FF FGFR VLVVSSPSAAE+CLSKND++FANRP L+ GKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEI S+HRLQTLSSIRVDEVRTLIRRLYN +NDVVDMRTEFFE+MFNI MR
Sbjct: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMRTEFFELMFNIMMR 180

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGED AA SEEA RFREIQEETFRLS KTNLGDFLR+VKW GLSKG+ENR++E
Sbjct: 181 MIAGKRYYGED-AAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKE 240

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300
           LQ KRD WMQSLIEEHRK+ N           DAKKTMIEVLLSLQQKEP YYKDEYIRG
Sbjct: 241 LQSKRDAWMQSLIEEHRKQSNK----------DAKKTMIEVLLSLQQKEPHYYKDEYIRG 300

Query: 301 LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL 360
           LMLVLLLAGTEGSINTMEWLLSLLLN+P  LQRAQ EIDDVVGR+NR+LEESDL HLPYL
Sbjct: 301 LMLVLLLAGTEGSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYL 360

Query: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN 420
           RSLIHETLRMYPPGPLLIPHESSEDCHVGGF VPAGT LFVNVWAIQNDPTVWVEP KFN
Sbjct: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVEPEKFN 420

Query: 421 PDRFGGDGEG--FKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSE 480
           P RFG +GEG  FKWMPFGAGRRRCPGEGLGLRV+GL VGSLIQCFEWES+ GECIDM E
Sbjct: 421 PGRFGSEGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMRE 480

Query: 481 GGGLTLPKALPLRTLCRPRSNATHLLSQI 507
           G GLTLPKA+PL  LCRPRS   HLL QI
Sbjct: 481 GVGLTLPKAVPLHALCRPRSKVAHLLPQI 498

[Close]

BLAST of Cucsat.G20977.T7 vs. NCBI nr
Match: XP_023535659.1 (isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 858 bits (2216), Expect = 1.67e-311
Identity = 428/509 (84.09%), Postives = 453/509 (89.00%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           M I FL LLIL +L IFTN FLHRIRNLPPSPF SLPIIGQLYLLK PLHRTLS+ISRRH
Sbjct: 1   MAITFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPI+FF FGFR VLVVSSPSAAE+CLSKND++FANRP L+ GKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRR+SSLEI S+HRLQTLSSIRVDEVRTLIRRLYN ENDVVDMRTEFFE+MFNI MR
Sbjct: 121 WRNLRRVSSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTENDVVDMRTEFFELMFNIMMR 180

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGED AA SEEA RFREIQEETFRLS KTNLGDFLR+VKW GLSKG+ENR++E
Sbjct: 181 MIAGKRYYGED-AAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKE 240

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300
           LQ KRD WMQSLIEEHRK+             DAKKTMIEVLLSLQQKEP+YYKDEYIRG
Sbjct: 241 LQSKRDAWMQSLIEEHRKQSKK----------DAKKTMIEVLLSLQQKEPRYYKDEYIRG 300

Query: 301 LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL 360
           LMLVLLLAGTEGSINTMEWLLSLLLN+P  LQRAQ+EIDDVVGR+NR+LEESDL HLPYL
Sbjct: 301 LMLVLLLAGTEGSINTMEWLLSLLLNNPECLQRAQLEIDDVVGRSNRILEESDLAHLPYL 360

Query: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN 420
           RSLIHETLRMYPPGPLLIPHESS DCHVGGF VPAGT LFVNVWAIQNDPTVWV+P KFN
Sbjct: 361 RSLIHETLRMYPPGPLLIPHESSADCHVGGFRVPAGTTLFVNVWAIQNDPTVWVDPEKFN 420

Query: 421 PDRFGGDGEG--FKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSE 480
           P RFG +GEG  FKWMPFGAGRRRCPGEGLGLRV+GL VGSLIQCFEWES+ GECIDM E
Sbjct: 421 PGRFGSEGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMRE 480

Query: 481 GGGLTLPKALPLRTLCRPRSNATHLLSQI 507
           G GLTLPKA+PL  LCRPRS   HLL QI
Sbjct: 481 GVGLTLPKAVPLHALCRPRSKVAHLLPQI 498

[Close]

BLAST of Cucsat.G20977.T7 vs. ExPASy TrEMBL
Match: A0A0A0L0F1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G642290 PE=3 SV=1)

HSP 1 Score: 1019 bits (2636), Expect = 0.0
Identity = 507/507 (100.00%), Postives = 507/507 (100.00%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH
Sbjct: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR
Sbjct: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE
Sbjct: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300
           LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG
Sbjct: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300

Query: 301 LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL 360
           LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL
Sbjct: 301 LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL 360

Query: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN 420
           RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN
Sbjct: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN 420

Query: 421 PDRFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEGG 480
           PDRFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEGG
Sbjct: 421 PDRFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEGG 480

Query: 481 GLTLPKALPLRTLCRPRSNATHLLSQI 507
           GLTLPKALPLRTLCRPRSNATHLLSQI
Sbjct: 481 GLTLPKALPLRTLCRPRSNATHLLSQI 507

[Close]

BLAST of Cucsat.G20977.T7 vs. ExPASy TrEMBL
Match: A0A1S3BVF3 (cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103493585 PE=3 SV=1)

HSP 1 Score: 948 bits (2450), Expect = 0.0
Identity = 473/508 (93.11%), Postives = 488/508 (96.06%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           MVI FL+LLILF+LQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLK PLHRTLSEISRRH
Sbjct: 31  MVISFLYLLILFILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEISRRH 90

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPI+FFQFGFR VLVVSSPSA EDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH
Sbjct: 91  GPILFFQFGFRPVLVVSSPSAVEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 150

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEILSSHRLQTLSSIRVDEVR LIRRLYNAENDVVDMRTEFFE+MFNI MR
Sbjct: 151 WRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAENDVVDMRTEFFELMFNIMMR 210

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGE V A SEE  RF+EIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE
Sbjct: 211 MIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 270

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSS-IQTDAKKTMIEVLLSLQQKEPQYYKDEYIR 300
           LQI+RDDWMQSLIEEHRKKM+NA+SSSSS IQ DAKKTMIEVLLSLQQ EPQ YKDEYIR
Sbjct: 271 LQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQNYKDEYIR 330

Query: 301 GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPY 360
           GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSL+RAQMEID+VVG+TNRLLEESDLTHLPY
Sbjct: 331 GLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDLTHLPY 390

Query: 361 LRSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKF 420
           LRSLIHETLRMYPPGPLLIPHESS+DCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEP KF
Sbjct: 391 LRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPEKF 450

Query: 421 NPDRFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEG 480
           NPDRF  DGEGFKWMPFGAGRRRCPGE LGLRV+GLV+GSLIQCFEWESM G+CIDMSEG
Sbjct: 451 NPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCIDMSEG 510

Query: 481 GGLTLPKALPLRTLCRPRSNATHLLSQI 507
           GGLTLPKALPLRTLCRPRSNATHLLSQI
Sbjct: 511 GGLTLPKALPLRTLCRPRSNATHLLSQI 538

[Close]

BLAST of Cucsat.G20977.T7 vs. ExPASy TrEMBL
Match: A0A6J1FED9 (isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111443311 PE=3 SV=1)

HSP 1 Score: 857 bits (2213), Expect = 2.31e-311
Identity = 429/509 (84.28%), Postives = 452/509 (88.80%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           M I FL LLIL +L IFTN FLHRIRNLPPSPF SLPIIGQLYLLK PLHRTLS+ISRRH
Sbjct: 1   MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPI+FF FGFR VLVVSSPSAAE+CLSKND++FANRP L+ GKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEI S+HRLQTLSSIRVDEVRTLIRRLYN +NDVVDMRTEFFE+MFNI MR
Sbjct: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMRTEFFELMFNIMMR 180

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGED AA SEEA RFREIQEETFRLS KTNLGDFLR+VKW GLSKG+ENR++E
Sbjct: 181 MIAGKRYYGED-AAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKE 240

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300
           LQ KRD WMQSLIEEHRK+             DAKKTMIEVLLSLQQKEP YYKDEYIRG
Sbjct: 241 LQSKRDAWMQSLIEEHRKQSKK----------DAKKTMIEVLLSLQQKEPHYYKDEYIRG 300

Query: 301 LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL 360
           LMLVLLLAGTEGSINTMEWLLSLLLN+P  LQRAQ EIDDVVGR+NR+LEESDL HLPYL
Sbjct: 301 LMLVLLLAGTEGSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYL 360

Query: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN 420
           RSLIHETLRMYPPGPLLIPHESSEDCHVGGF VPAGT LFVNVWAIQNDPTVWV+P KFN
Sbjct: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVDPEKFN 420

Query: 421 PDRFGGDGEG--FKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSE 480
           P RFG +GEG  FKWMPFGAGRRRCPGEGLGLRV+GL VGSLIQCFEWES+ GECIDM E
Sbjct: 421 PGRFGSEGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMRE 480

Query: 481 GGGLTLPKALPLRTLCRPRSNATHLLSQI 507
           G GLTLPKA+PL  LCRPRS   HLL QI
Sbjct: 481 GVGLTLPKAVPLHALCRPRSKVAHLLPQI 498

[Close]

BLAST of Cucsat.G20977.T7 vs. ExPASy TrEMBL
Match: A0A6J1INS3 (isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111477207 PE=3 SV=1)

HSP 1 Score: 853 bits (2205), Expect = 3.82e-310
Identity = 427/509 (83.89%), Postives = 451/509 (88.61%), Query Frame = 0


Query: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60
           M I FL LLILF+L IFTN FLHRIRNLPPSPF SLPIIGQLYLLK PLHRTL +ISRRH
Sbjct: 1   MAITFLCLLILFILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLLDISRRH 60

Query: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120
           GPI+FF FGFR VLVVSSPSAAE+CLSKND++FANRP L+ GKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120

Query: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180
           WRNLRRISSLEI S+HRLQTLSSIRVDEVRTLIRRLYN +NDVVDM+TEFFE+MFNI MR
Sbjct: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMKTEFFELMFNIMMR 180

Query: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240
           MIAGKRYYGEDVA  SEEA RFREIQEETFRLS KTNLGDFLR+VKW GLSKG+ENR++E
Sbjct: 181 MIAGKRYYGEDVAE-SEEAERFREIQEETFRLSSKTNLGDFLRMVKWAGLSKGLENRMKE 240

Query: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300
           LQ KRD WMQSLIEEHRK+             DAKKTMIEVLLSLQQKEP YYKDEYIRG
Sbjct: 241 LQSKRDAWMQSLIEEHRKQSKK----------DAKKTMIEVLLSLQQKEPHYYKDEYIRG 300

Query: 301 LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL 360
           LMLVLLLAGTEGSINTMEWLLSLLLN+P  LQRAQ EIDDVVGR+NR+LEESDL HLPYL
Sbjct: 301 LMLVLLLAGTEGSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYL 360

Query: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN 420
           RSLIHETLRMYPPGPLLIPHESS DCHVGGF VPAGT LFVNVWAIQNDPTVWV+P KFN
Sbjct: 361 RSLIHETLRMYPPGPLLIPHESSADCHVGGFRVPAGTTLFVNVWAIQNDPTVWVDPEKFN 420

Query: 421 PDRFGGDGEG--FKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSE 480
           P RFG +GEG  FKWMPFGAGRRRCPGEGLGLRV+GL VGSLIQCFEWES+ GECIDM E
Sbjct: 421 PGRFGSEGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMRE 480

Query: 481 GGGLTLPKALPLRTLCRPRSNATHLLSQI 507
           G GLTLPKA+PL  LCRPRS   HLL QI
Sbjct: 481 GVGLTLPKAVPLHALCRPRSKVAHLLPQI 498

[Close]

BLAST of Cucsat.G20977.T7 vs. ExPASy TrEMBL
Match: A0A7J0EHR3 (Cytochrome P450 superfamily protein OS=Actinidia rufa OX=165716 GN=Acr_04g0007630 PE=3 SV=1)

HSP 1 Score: 644 bits (1662), Expect = 1.70e-227
Identity = 321/495 (64.85%), Postives = 395/495 (79.80%), Query Frame = 0


Query: 16  IFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRHGPIVFFQFGFRSVLV 75
           I   HFLH+IRNLPPSPF +LPI G LYLLKTP+HRTLS IS  HGPI+  +FGFR VL+
Sbjct: 16  IIAKHFLHKIRNLPPSPFPALPIFGHLYLLKTPIHRTLSNISAHHGPILLLRFGFRPVLL 75

Query: 76  VSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEHWRNLRRISSLEILSS 135
           VSSPSAAE+CLSKND++FANRPRL++GK+LG NY +L WAPYG  WRNLRRI+++EILSS
Sbjct: 76  VSSPSAAEECLSKNDIVFANRPRLLAGKHLGQNYNTLSWAPYGARWRNLRRIAAVEILSS 135

Query: 136 HRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMRMIAGKRYYGEDVAAY 195
           HRLQTLSSIR DEVR+LIRRL    ++ VDM+  FFE+M N+TMRMIAGK YYGEDVA  
Sbjct: 136 HRLQTLSSIRADEVRSLIRRLARG-SEGVDMKAAFFELMLNVTMRMIAGKTYYGEDVAE- 195

Query: 196 SEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRELQIKRDDWMQSLIEE 255
           +E A RFREI  ETF L+G +N GDFL V+KW+G S G+E RL  L+ KRD++MQ +IEE
Sbjct: 196 AEAARRFREIVTETFVLAGASNFGDFLPVMKWIGAS-GLEKRLIALREKRDEFMQEMIEE 255

Query: 256 HRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRGLMLVLLLAGTEGSIN 315
            R+++    S       + KKTM+EVLLSLQ+ EP++Y D+ IRG+M VLL AGT+ S+ 
Sbjct: 256 QRRRIGGGGSE------EKKKTMVEVLLSLQETEPEHYTDQMIRGMMSVLLAAGTDTSVG 315

Query: 316 TMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYLRSLIHETLRMYPPGP 375
           TMEW LSLLLN P++LQ+AQ EID  VG+ +RL++ESD+  LPYLR +I+ET+RMYP GP
Sbjct: 316 TMEWALSLLLNQPYALQKAQTEIDKQVGQ-HRLIDESDIADLPYLRCIINETMRMYPAGP 375

Query: 376 LLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFNPDRFGG-DG--EGFK 435
           LL+PHESSEDC VGGFH+P GTML VN+WAIQNDP +WVEP KF P+RF G +G  +GFK
Sbjct: 376 LLVPHESSEDCMVGGFHIPRGTMLLVNMWAIQNDPKIWVEPNKFKPERFEGLEGARDGFK 435

Query: 436 WMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEGGGLTLPKALPLRT 495
           ++PFG+GRR CPGEGL LR++GL +GSLIQCF+WE +  E +DM+EG GLTLPKA PL  
Sbjct: 436 FVPFGSGRRVCPGEGLALRMVGLALGSLIQCFDWERVGKEMVDMTEGTGLTLPKARPLMA 495

Query: 496 LCRPRSNATHLLSQI 507
            CRPR    ++LSQI
Sbjct: 496 KCRPRLTMVNILSQI 500

The following BLAST results are available for this feature:

BLAST of Cucsat.G20977.T7 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
W8JMU7	3.4e-134	48.53	Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1	[more]
P93147	3.0e-122	45.25	Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2	[more]
Q6WNQ8	1.7e-120	46.22	Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1	[more]
Q6WNQ9	6.3e-120	46.00	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P...	[more]
Q0WTF4	2.4e-119	45.78	Cytochrome P450 81F3 OS=Arabidopsis thaliana OX=3702 GN=CYP81F3 PE=2 SV=1	[more]

BLAST of Cucsat.G20977.T7 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004141396.1	0.0	100.00	cytochrome P450 81Q32 [Cucumis sativus] >XP_011654142.1 cytochrome P450 81Q32 [C...	[more]
XP_008452618.1	0.0	93.11	PREDICTED: cytochrome P450 81E8-like [Cucumis melo]	[more]
XP_038899302.1	0.0	88.36	cytochrome P450 81Q32-like [Benincasa hispida]	[more]
KAG7024285.1	2.03e-312	84.68	Isoflavone 2'-hydroxylase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023535659.1	1.67e-311	84.09	isoflavone 2'-hydroxylase-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cucsat.G20977.T7 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L0F1	0.0	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G642290 PE=3 SV=1	[more]
A0A1S3BVF3	0.0	93.11	cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103493585 PE=3 SV=1	[more]
A0A6J1FED9	2.31e-311	84.28	isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111443311 PE=...	[more]
A0A6J1INS3	3.82e-310	83.89	isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111477207 PE=3 ...	[more]
A0A7J0EHR3	1.70e-227	64.85	Cytochrome P450 superfamily protein OS=Actinidia rufa OX=165716 GN=Acr_04g000763...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 434..443 score: 60.07 coord: 362..373 score: 40.54 coord: 308..325 score: 31.08 coord: 443..454 score: 31.36
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 30..485 e-value: 1.4E-87 score: 294.4
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 81..102 score: 25.45 coord: 361..379 score: 34.78 coord: 297..314 score: 26.01 coord: 317..343 score: 36.21 coord: 443..466 score: 33.8 coord: 57..76 score: 28.43 coord: 402..426 score: 35.4 coord: 433..443 score: 57.48 coord: 173..191 score: 24.18
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 23..505 e-value: 1.8E-118 score: 398.2
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 29..497
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 12..472 e-value: 1.2E-15 score: 55.3
None	No IPR available	PANTHER	PTHR47947:SF24	CYTOCHROME P450 81E8	coord: 13..506
None	No IPR available	PANTHER	PTHR47947	CYTOCHROME P450 82C3-RELATED	coord: 13..506
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 436..445

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G20977	Cucsat.G20977	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G20977.T7.E1	Cucsat.G20977.T7.E1	exon
Cucsat.G20977.T7.E2	Cucsat.G20977.T7.E2	exon
Cucsat.G20977.T7.E3	Cucsat.G20977.T7.E3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G20977.T7.C1	Cucsat.G20977.T7.C1	CDS
Cucsat.G20977.T7.C2	Cucsat.G20977.T7.C2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G20977.T7	Cucsat.G20977.T7-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0098542	defense response to other organism
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen