Cucsat.G13525 (gene) Cucumber (B10) v3

Overview
NameCucsat.G13525
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionProtein kinase domain-containing protein
Locationctg1838: 8304664 .. 8307611 (+)
RNA-Seq ExpressionCucsat.G13525
SyntenyCucsat.G13525
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTCGGGTCGTCGGGGTCGGGTCGGAAAGGGGCAAAAGCGGTAGAGTCGTGGTGATGTGCAGTGGAGTACGGAGTGGACACGTTGGTTGGACATAATAAAGTTTTTGGAGACCAAAAGAAAACCCCTAAATTGCTTTAGATTTTTATTAAATTTAATTTTGTTTTCCTATTTTTCTCTTGGGGTTGGGGGGATATTTTGGCTGTTTCCATATATTTTTTCTATTGTCGTTTATAAATCGGGGGGATTGCTGGATTTTCTCCCAAAGGGTAATGTGATCGAAGATTGCTAGGGTTTCGGATTCTGTGTTCTTCATCGTCTATTTCTTTAATGGATCGATCTTTGTGTTAATGGTGGTACTGGTTGTTTTGTTTAAGGTCCTCAGAACCGGCGATTTGATATGCGAGTGGTGGTGAAAGATATCTGGGGCTTTGAAGGTGCAATTTTATCATCTGGGTCTTTCTTGTTTTCTTTTGAAGTTTATGGAATTTGACGATGTAGATCTTTATATGAACTGGGGTTTGGGTTGAGCTTACGACTAATTGATTATGATATTTTGTTTGTTTTGGGGTATGTAATGTTAGTTACGATATGCAATCATTGATCTGTTTGCATTTTTTAAACGTGAGTTGAGAATCGGTGAATTTCTTGCAGTTTTCAGTTGTACGAAATCGGGGGTAAGAGCTGCTGGCTGATTAATTTGATTTGATGAGGAAGAAAAGGAAAGGAAGTGAGACTGATGGACCGTTAAATTTACAAGACAATGTGACAGAATCTCATGAATCGCGATCTTCTAAACTGAAGGCTCATTACTCGCTAGAAGACTATACGAGGTTGAATAAAAGGTGCAAGGAAGATGTGGGTATTGAACCGGTTCTTTCTTGTAAGAGTAGATTTGCAGGTATAGCTACAGCTCCACCCAGTGGGAGTTCGTCTCTGATTCTTCCTGGTAGAGGTTTGAAGAGGAAGATCGGATGCATAGACGTGGCTACTCAAATCGGGCGGAAACACAACATTGAGAATGATTATGTTTCAGGTAAGACTATTGGGCATGGAAAATTTGGCTCAGTTTGGCTATGTAAATGTAAGGTTAGTGGAGCTGAGTATGCTTGCAAGACCTTGAAAAAAGGGGAAGAAACTGTACATAGGGAGGTGGAGATAATGCAGCACTTATCTGGCTACCCTGGTGTTGTGACATTGTTGGCAGTGTACGAGGACTCCGAATGTTTTCACCTGGTTATGGAACTGTGTGGAGGAGGCCGTTTGGTCGAACAGATGGGCAGTGAAGGCCAATATTCAGAGCATCGAGCAGCCAATATACTAAAGGAAGTGATGTTGGTTATAAAATATTGTCATGATATGGGGGTTGTACATAGAGACATAAAGCCTGAAAATATTCTTCTCACAACATCTGGAAAGATAAAGCTTGCAGATTTTGGCCTGGCCACTAGGATTTCATATGGTATGCATTCTTTAATCTGTAATAGAGAGTACATCTTTGTATGTTTCTCTTCAAGTATTAGTTGCCTGATCTTCTTTGTAGTCTTCAATTGCAGACATCCAATGTTTATTGATCACCAGCTATGGAAGTGTTCACATTTTTTTGTTTCTTTTGTTACCTTTGCTTGTATCTTTCTGTGACCGTTCAAATGTCTTGTAAAGAAATAAGTGAGGAATGTCACTACAACTTAGTAGTATTTGAAGCACTGTAAATACAGCACTACAAAGTTATTAGATGAAATATGCTCAACAAACAAAGAAAGAAAATTTACGATCAAACTAAAGAAGTTGCTTAAAATTCCAATTAGCAAAAATAGATGTTGAACTATAGTCATAAAAAAAAAAGTTGTTAATTTTACTCCTTGCGTCACCCAATCCTGTTAGTCTATGGTTCCTTGGATAACCATTGTTCAGATTCAAGGTATGCTTATGCATTACAAGAATGATTGGACCGTAGCTCACATTTGGTGTTTAGCGACAATGCGTTAGCTTAGAGTGAGAGGATGCTTGTAAGGAGAGAAATTACTTTGGGAGTAAATTTTTTTTTGAGGGCCAAATGCTATGCTTGTAGAGAGATCAGATGTTGAGAGGGTGGGCTAAGCTTTATGAGCAGAACATCCATTTCAAATTGGACCCCTATGCTTTCACAACTTTGATGTATTGTTATTAAGATCACGTTTCCAAACGCTGGCCTAAACAGATTATTGTTTCCAACAATATATTCCAAAAACAAGTTTGTCAAGTGAGTTTAGTGTATTTAGGAGCATTTTTAGAAAACATAAAATATAAACTGATTAGGTACCACAATCCTGTGAGGTAAGACTTAATTGAATGGACAATGATATCGTAGATTGCAGCATTACATAAATTTTACACCATAATAGTTTTCTATTATATATTATGTTTCTTGCTTCTACTTTCTTGTAATGATGCCACTTGTATATTTCTCATTGTTGCTATCAACGGTGTGCAAATTTTGATGGTCTTTTACATTTAGAATAAGAGTTCCAAACATCTACTTATTTAAGTTATTCGACTGCGTTCTGCAGGCCAGAGTTTAACTGGTTTGGCTGGAAGCCCAGCTTATGTAGCCCCTGAAGTGTTGACAGGAAAGTACTCTGAGAAGGTGGATATTTGGAGTGCCGGTGTGCTCCTCCATGCTCTTTTGGTTGGCACTCTTCCTTTCCAAGGTGACTCATTAGAATCAGTTTTCGAAGCAATAAAGAACTCTAAGCTTGACTTCCACTCAGGGATGTGGGAGTCCATATCTAAACCTGCCCGCGATCTCATTGGGAGAATGCTGACAAGAGATATTTCAGCTAGGATAACAGCAGAAGAAGTCTTACGTAAGTTTGTTTTACTAATTGGTTTCATGCCCTGATGGTTAAGTAATACATAATCAGTTTTATATGCTATCGATATTTTGATATTCTGAAACAAGCATAAACCATTTGCTATGTTGTTATAAAGTCCAATATCCTTAAATAGATTGATGCCAATCTTAATCTTTTTAGTGTAATCTGATTTGGATCCAAAATGGTGATATTTCAGGACATCCCTGGATATTGTTTTATACCGAACGCACGTTGAAGGCTCTCCCGATCAAACTAAAATTGAAGAATCAAGAATCATCCCAACAGTCACCCATTGCAGCCAAAATTAAGTCTGACAGAAACAGGATTGATAGTGCAGCTAATATGACCTCCCTTAACGAAGTCTCTAACCTTTCCTCATCCGAGAGTTGCAATGCTGATGGTGATGATGTCGACGATTGCTTTCTGGTGGATGCACTTGCTACAGCTATTTCACATGTGAGAATTTCCGAGCCAAAGAGAAGTAGACTATGTGTTCCTACAGGACCTATCGAGCAGCACAGCTCCTCTAACATGAAAGCTAACAATCTTTGTAAAGCGTTTTGATCCTGCTGACAGGCCAACAACGACATCGTCAATACTGTTCTCTCCTGTTTTTTTTTACCTGAGCTGGGGGAACTACTACTTACAGGTAACACAACCCGGATGAATTCCTCCAAGTAAGCTAAAGAAGGTGATTTCCAGGCTAACATCAATGAAATCAATCCACAATATGATTTTTTCTTTTTTAATATGCAGTATGTGTTAGTCCATGCCTGAGGTGCATTAAATCTGATTAGTAGTGTGTGATTTTGTGCTTTAAGTCTCTAATAATTGTCTTAACAAGAACTGACAAATTTGATGTACATATTCTTGTAAATACAGCAGTAATGAAGAGTGCATTTTGATATTTTCGGTCTCCAATGCCCAAATTGGCTGCAGGAACCACACAGCAAATCTAAAGCCGTTGAGGCTCAATTTCTACTTCCTCTATATCTGTTATCTCAATTGAAAGTTTAGGTTTTACATTATTACTAATAGGTCACGTTTTCAAATCGTAATGAAAATGGGTGTATTTGGATTGAGATTCTATTGAATTATTGATGGGTAATGAAAATGGGTGTATTTGGATTGAGATTCTATTGGATTATTGATGGGTAAATCGCA

Coding sequence (CDS)

ATGAGGAAGAAAAGGAAAGGAAGTGAGACTGATGGACCGTTAAATTTACAAGACAATGTGACAGAATCTCATGAATCGCGATCTTCTAAACTGAAGGCTCATTACTCGCTAGAAGACTATACGAGGTTGAATAAAAGGTGCAAGGAAGATGTGGGTATTGAACCGGTTCTTTCTTGTAAGAGTAGATTTGCAGGTATAGCTACAGCTCCACCCAGTGGGAGTTCGTCTCTGATTCTTCCTGGTAGAGGTTTGAAGAGGAAGATCGGATGCATAGACGTGGCTACTCAAATCGGGCGGAAACACAACATTGAGAATGATTATGTTTCAGGTAAGACTATTGGGCATGGAAAATTTGGCTCAGTTTGGCTATGTAAATGTAAGGTTAGTGGAGCTGAGTATGCTTGCAAGACCTTGAAAAAAGGGGAAGAAACTGTACATAGGGAGGTGGAGATAATGCAGCACTTATCTGGCTACCCTGGTGTTGTGACATTGTTGGCAGTGTACGAGGACTCCGAATGTTTTCACCTGGTTATGGAACTGTGTGGAGGAGGCCGTTTGGTCGAACAGATGGGCAGTGAAGGCCAATATTCAGAGCATCGAGCAGCCAATATACTAAAGGAAGTGATGTTGGTTATAAAATATTGTCATGATATGGGGGTTGTACATAGAGACATAAAGCCTGAAAATATTCTTCTCACAACATCTGGAAAGATAAAGCTTGCAGATTTTGGCCTGGCCACTAGGATTTCATATGGCCAGAGTTTAACTGGTTTGGCTGGAAGCCCAGCTTATGTAGCCCCTGAAGTGTTGACAGGAAAGTACTCTGAGAAGGTGGATATTTGGAGTGCCGGTGTGCTCCTCCATGCTCTTTTGGTTGGCACTCTTCCTTTCCAAGGTGACTCATTAGAATCAGTTTTCGAAGCAATAAAGAACTCTAAGCTTGACTTCCACTCAGGGATGTGGGAGTCCATATCTAAACCTGCCCGCGATCTCATTGGGAGAATGCTGACAAGAGATATTTCAGCTAGGATAACAGCAGAAGAAGTCTTACGACATCCCTGGATATTGTTTTATACCGAACGCACGTTGAAGGCTCTCCCGATCAAACTAAAATTGAAGAATCAAGAATCATCCCAACAGTCACCCATTGCAGCCAAAATTAAGTCTGACAGAAACAGGATTGATAGTGCAGCTAATATGACCTCCCTTAACGAAGTCTCTAACCTTTCCTCATCCGAGAGTTGCAATGCTGATGGTGATGATGTCGACGATTGCTTTCTGGTGGATGCACTTGCTACAGCTATTTCACATGTGAGAATTTCCGAGCCAAAGAGAAGTAGACTATGTGTTCCTACAGGACCTATCGAGCAGCACAGCTCCTCTAACATGAAAGCTAACAATCTTTGTAAAGCGTTTTGA

Protein sequence

MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCKSRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGSVWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMELCGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKLADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGDSLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTERTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLNEVSNLSSSESCNADGDDVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF
Homology
BLAST of Cucsat.G13525 vs. ExPASy Swiss-Prot
Match: Q8W490 (Serine/threonine-protein kinase PEPKR2 OS=Arabidopsis thaliana OX=3702 GN=PEPKR2 PE=2 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 2.4e-145
Identity = 253/351 (72.08%), Postives = 301/351 (85.75%), Query Frame = 0

Query: 1   MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSET+G  NL D+++ S  SRSS  ++H+SLE Y RL KRCKE   +E V S K
Sbjct: 1   MRKKRKGSETEGWENLPDDLSCSTASRSSNFRSHFSLEGYARLKKRCKETEAVESVGSFK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
            R AG+ATAPP G+SSL+  GRGLKRKIGCIDV+TQ GRK+ I++DYV G+ IG GKFGS
Sbjct: 61  RRIAGVATAPPCGASSLVSSGRGLKRKIGCIDVSTQTGRKNKIDDDYVFGRNIGKGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL 180
           V +CK + +G E+ACKTLKKGEETVHREVEIMQHLSG+P VVTL AVYE+S+CFHLVMEL
Sbjct: 121 VRICKSRKNGTEFACKTLKKGEETVHREVEIMQHLSGHPRVVTLHAVYEESDCFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           C GGRL++QM   G+YSE RAANI K++MLVI YCH+MGVVHRDIKPENILLT +GKI+L
Sbjct: 181 CSGGRLIDQMVKVGRYSEQRAANIFKDLMLVINYCHEMGVVHRDIKPENILLTAAGKIQL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLA RI+ GQ+L+GLAGSPAYVAPEVL+  YSEKVD+WSAGVLL+ALL G LPF+GD
Sbjct: 241 ADFGLAMRIAKGQTLSGLAGSPAYVAPEVLSENYSEKVDVWSAGVLLYALLSGVLPFKGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLR 352
           SL+++FEAIKN KLDF++G+WES+SKPARDL+ RMLTR+ SARITA+EVLR
Sbjct: 301 SLDAIFEAIKNVKLDFNTGVWESVSKPARDLLARMLTREESARITADEVLR 351

BLAST of Cucsat.G13525 vs. ExPASy Swiss-Prot
Match: Q6F3A6 (Calcium-dependent protein kinase 10 OS=Oryza sativa subsp. japonica OX=39947 GN=CPK10 PE=2 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 7.8e-64
Identity = 122/288 (42.36%), Postives = 181/288 (62.85%), Query Frame = 0

Query: 74  SSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGSVWLCKCKVSGAEY 133
           SS+ +L G  LKRK              ++++ Y  G+ +G G+FG+ +LC  + +G E+
Sbjct: 112 SSAGLLVGSVLKRK------------TESLKDKYSLGRKLGQGQFGTTYLCVERATGKEF 171

Query: 134 ACKTLKKGE-------ETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMELCGGGRL 193
           ACK++ K +       E V RE++IM HL+G+P V+++   YED+   HLVMELC GG L
Sbjct: 172 ACKSILKRKLVTDDDVEDVRREIQIMYHLAGHPNVISIRGAYEDAVAVHLVMELCAGGEL 231

Query: 194 VEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILL---TTSGKIKLADF 253
            +++  +G Y+E +AA + + ++ V++ CH MGV+HRD+KPEN L    T    +K  DF
Sbjct: 232 FDRIVQKGHYTERKAAELARVIVGVVEVCHSMGVMHRDLKPENFLFADQTEEAALKTIDF 291

Query: 254 GLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGDSLE 313
           GL+     GQ  T + GSP YVAPEVL  KY ++ D+WSAGV+++ LL G  PF  ++ +
Sbjct: 292 GLSIFFRPGQVFTDVVGSPYYVAPEVLKKKYGQEADVWSAGVIIYILLCGVPPFWAENEQ 351

Query: 314 SVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLR 352
            +FE + + +LDF S  W SIS+ A+DL+ RML RD   R+TA EVLR
Sbjct: 352 GIFEEVLHGRLDFQSEPWPSISEGAKDLVRRMLVRDPKKRLTAHEVLR 387

BLAST of Cucsat.G13525 vs. ExPASy Swiss-Prot
Match: Q9ZV15 (Calcium-dependent protein kinase 20 OS=Arabidopsis thaliana OX=3702 GN=CPK20 PE=3 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 1.2e-61
Identity = 114/271 (42.07%), Postives = 174/271 (64.21%), Query Frame = 0

Query: 91  IDVATQIGRK-HNIENDYVSGKTIGHGKFGSVWLCKCKVSGAEYACKTLKKGE------- 150
           + + + +GRK  N+++ Y  G+ +G G+FG+ +LC  K +G E+ACKT+ K +       
Sbjct: 117 LQIDSVLGRKTENLKDIYSVGRKLGQGQFGTTFLCVDKKTGKEFACKTIAKRKLTTPEDV 176

Query: 151 ETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMELCGGGRLVEQMGSEGQYSEHRAA 210
           E V RE++IM HLSG+P V+ ++  YED+   H+VME+C GG L +++   G Y+E +AA
Sbjct: 177 EDVRREIQIMHHLSGHPNVIQIVGAYEDAVAVHVVMEICAGGELFDRIIQRGHYTEKKAA 236

Query: 211 NILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGK---IKLADFGLATRISYGQSLTGLA 270
            + + ++ VI+ CH +GV+HRD+KPEN L  +  +   +K  DFGL+     G++ T + 
Sbjct: 237 ELARIIVGVIEACHSLGVMHRDLKPENFLFVSGDEEAALKTIDFGLSVFFKPGETFTDVV 296

Query: 271 GSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGDSLESVFEAIKNSKLDFHSG 330
           GSP YVAPEVL   YS + D+WSAGV+++ LL G  PF  ++ + +FE +    LDF S 
Sbjct: 297 GSPYYVAPEVLRKHYSHECDVWSAGVIIYILLSGVPPFWDETEQGIFEQVLKGDLDFISE 356

Query: 331 MWESISKPARDLIGRMLTRDISARITAEEVL 351
            W S+S+ A+DL+ RML RD   R+T  EVL
Sbjct: 357 PWPSVSESAKDLVRRMLIRDPKKRMTTHEVL 387

BLAST of Cucsat.G13525 vs. ExPASy Swiss-Prot
Match: P53684 (Calcium-dependent protein kinase 7 OS=Oryza sativa subsp. japonica OX=39947 GN=CPK7 PE=2 SV=2)

HSP 1 Score: 237.3 bits (604), Expect = 2.8e-61
Identity = 117/266 (43.98%), Postives = 167/266 (62.78%), Query Frame = 0

Query: 97  IGRK-HNIENDYVSGKTIGHGKFGSVWLCKCKVSGAEYACKTL-------KKGEETVHRE 156
           +GRK  ++   Y+ G+ +G G+FG+ +LC    +G EYACKT+       K+  E V RE
Sbjct: 68  LGRKTADLREHYIIGRKLGQGQFGTTYLCTEINTGCEYACKTIPKRKLITKEDVEDVRRE 127

Query: 157 VEIMQHLSGYPGVVTLLAVYEDSECFHLVMELCGGGRLVEQMGSEGQYSEHRAANILKEV 216
           ++IM HLSG+  VV +  VYED +  H+VMELC GG L +++  +G YSE +AA +++ +
Sbjct: 128 IQIMHHLSGHKNVVAIKDVYEDGQAVHIVMELCAGGELFDRIQEKGHYSERKAAELIRII 187

Query: 217 MLVIKYCHDMGVVHRDIKPENILLTTSG---KIKLADFGLATRISYGQSLTGLAGSPAYV 276
           + ++  CH +GV+HRD+KPEN LL        IK  DFGL+     GQ  T L GSP YV
Sbjct: 188 VSIVAMCHSLGVMHRDLKPENFLLLDKDDDLSIKAIDFGLSVFFKPGQVFTELVGSPYYV 247

Query: 277 APEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGDSLESVFEAIKNSKLDFHSGMWESIS 336
           APEVL  +Y  + D+WSAGV+L+ LL G  PF  ++ + +F+A+    +DF S  W  IS
Sbjct: 248 APEVLHKRYGPESDVWSAGVILYVLLSGVPPFWAETQQGIFDAVLKGHIDFQSDPWPKIS 307

Query: 337 KPARDLIGRMLTRDISARITAEEVLR 352
             A+DLI +ML+   S R+ A EVLR
Sbjct: 308 DSAKDLIRKMLSHCPSERLKAHEVLR 333

BLAST of Cucsat.G13525 vs. ExPASy Swiss-Prot
Match: P49101 (Calcium-dependent protein kinase 2 OS=Zea mays OX=4577 GN=CPK2 PE=2 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 6.2e-61
Identity = 116/268 (43.28%), Postives = 171/268 (63.81%), Query Frame = 0

Query: 95  TQIGRKH-NIENDYVSGKTIGHGKFGSVWLCKCKVSGAEYACKTLKK-------GEETVH 154
           T +G+++ ++ + Y  GK +G G+FG  +LC    SG +YACK++ K         E + 
Sbjct: 52  TILGKQYEDVRSVYSFGKELGRGQFGVTYLCTEIASGRQYACKSISKRKLVSKADREDIR 111

Query: 155 REVEIMQHLSGYPGVVTLLAVYEDSECFHLVMELCGGGRLVEQMGSEGQYSEHRAANILK 214
           RE++IMQHLSG P +V     YED    H+VMELC GG L +++ ++G Y+E  AA I +
Sbjct: 112 REIQIMQHLSGQPNIVEFRGAYEDKSNVHVVMELCAGGELFDRIIAKGHYTERAAATICR 171

Query: 215 EVMLVIKYCHDMGVVHRDIKPENILLTT---SGKIKLADFGLATRISYGQSLTGLAGSPA 274
            V+ V+  CH MGV+HRD+KPEN LL T   +  +K  DFGL+  I  G+    + GS  
Sbjct: 172 AVVNVVNICHFMGVMHRDLKPENFLLATMEENAMLKATDFGLSVFIEEGKMYRDIVGSAY 231

Query: 275 YVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGDSLESVFEAIKNSKLDFHSGMWES 334
           YVAPEVL   Y +++D+WSAGV+L+ LL G  PF  +  + +F+AI + ++DF S  W S
Sbjct: 232 YVAPEVLRRSYGKEIDVWSAGVILYILLSGVPPFWAEIEKGIFDAILHEEIDFESQPWPS 291

Query: 335 ISKPARDLIGRMLTRDISARITAEEVLR 352
           IS+ A+DL+ +MLTRD   R+T+ +VL+
Sbjct: 292 ISESAKDLVRKMLTRDPKKRLTSAQVLQ 319

BLAST of Cucsat.G13525 vs. NCBI nr
Match: XP_031738766.1 (serine/threonine-protein kinase PEPKR2 [Cucumis sativus] >KGN60241.1 hypothetical protein Csa_002436 [Cucumis sativus])

HSP 1 Score: 706 bits (1823), Expect = 1.32e-254
Identity = 351/351 (100.00%), Postives = 351/351 (100.00%), Query Frame = 0

Query: 1   MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK
Sbjct: 1   MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
           SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS
Sbjct: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL 180
           VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL
Sbjct: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL
Sbjct: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD
Sbjct: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLR 351
           SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLR
Sbjct: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLR 351

BLAST of Cucsat.G13525 vs. NCBI nr
Match: XP_008466187.1 (PREDICTED: serine/threonine-protein kinase PEPKR2 [Cucumis melo] >KAA0038682.1 serine/threonine-protein kinase PEPKR2 [Cucumis melo var. makuwa] >TYK31292.1 serine/threonine-protein kinase PEPKR2 [Cucumis melo var. makuwa])

HSP 1 Score: 692 bits (1787), Expect = 4.02e-249
Identity = 343/351 (97.72%), Postives = 348/351 (99.15%), Query Frame = 0

Query: 1   MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSETDGP NLQDNVTESH+SRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK
Sbjct: 1   MRKKRKGSETDGPSNLQDNVTESHDSRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
           SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSG+TIGHGKFGS
Sbjct: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGETIGHGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL 180
           VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYE+SECFHLVMEL
Sbjct: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEESECFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           C GGRLV+QMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL
Sbjct: 181 CRGGRLVDQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKY+EKVDIWSAGVLLHALLVG LPFQGD
Sbjct: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYTEKVDIWSAGVLLHALLVGALPFQGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLR 351
           SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLR
Sbjct: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLR 351

BLAST of Cucsat.G13525 vs. NCBI nr
Match: XP_038899232.1 (serine/threonine-protein kinase PEPKR2 [Benincasa hispida] >XP_038899233.1 serine/threonine-protein kinase PEPKR2 [Benincasa hispida])

HSP 1 Score: 681 bits (1757), Expect = 1.49e-244
Identity = 337/351 (96.01%), Postives = 344/351 (98.01%), Query Frame = 0

Query: 1   MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSETDGP NL DNVTESH+SRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK
Sbjct: 1   MRKKRKGSETDGPSNLPDNVTESHDSRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
           SRFAGIATAPPSGSSSLILPGRGLKRKIGCID+ATQIGRKHNIENDYVSG+TIGHGKFGS
Sbjct: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDMATQIGRKHNIENDYVSGETIGHGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL 180
           VWLCKCK SG EYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYE++ECFHLVMEL
Sbjct: 121 VWLCKCKASGTEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEEAECFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           C GGRLV+QM SEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL
Sbjct: 181 CRGGRLVDQMVSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLATRISYGQSLTGLAGSPAYVAPEVL+GKYSEKVDIWSAGVLLHALLVG LPFQGD
Sbjct: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLSGKYSEKVDIWSAGVLLHALLVGALPFQGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLR 351
           SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLR
Sbjct: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLR 351

BLAST of Cucsat.G13525 vs. NCBI nr
Match: XP_022976306.1 (serine/threonine-protein kinase PEPKR2 [Cucurbita maxima])

HSP 1 Score: 679 bits (1753), Expect = 6.05e-244
Identity = 337/351 (96.01%), Postives = 345/351 (98.29%), Query Frame = 0

Query: 1   MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSETDGPLNL+DNVTESH+SRSSKLKAHYSLEDYTRLNKRCKEDVGIEPV SCK
Sbjct: 1   MRKKRKGSETDGPLNLRDNVTESHDSRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVPSCK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
           SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSG+TIGHGKFGS
Sbjct: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGETIGHGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL 180
           VWLCKCKVSGAEYACKTLKK EETVHREVEIMQHLSGYPGVVTLLAVYE+SECFHLVMEL
Sbjct: 121 VWLCKCKVSGAEYACKTLKKAEETVHREVEIMQHLSGYPGVVTLLAVYEESECFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           C GGRLV+QM SEG+YSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL
Sbjct: 181 CRGGRLVDQMVSEGRYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLATRISYGQSL GLAGSPAYVAPEVL+GKYSEKVDIWSAGVLLHALLVG LPFQGD
Sbjct: 241 ADFGLATRISYGQSLIGLAGSPAYVAPEVLSGKYSEKVDIWSAGVLLHALLVGALPFQGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLR 351
           SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDIS+RITAEEVLR
Sbjct: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISSRITAEEVLR 351

BLAST of Cucsat.G13525 vs. NCBI nr
Match: XP_023535980.1 (serine/threonine-protein kinase PEPKR2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 679 bits (1753), Expect = 6.05e-244
Identity = 337/351 (96.01%), Postives = 345/351 (98.29%), Query Frame = 0

Query: 1   MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSETDGPLNL+DNVTESH+SRSSKLKAHYSLEDYTRLNKRCKEDVGIEPV SCK
Sbjct: 1   MRKKRKGSETDGPLNLRDNVTESHDSRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVPSCK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
           SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSG+TIGHGKFGS
Sbjct: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGETIGHGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL 180
           VWLCKCKVSGAEYACKTLKK EETVHREVEIMQHLSGYPGVVTLLAVYE+SECFHLVMEL
Sbjct: 121 VWLCKCKVSGAEYACKTLKKAEETVHREVEIMQHLSGYPGVVTLLAVYEESECFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           C GGRLV+QM SEG+YSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL
Sbjct: 181 CRGGRLVDQMVSEGRYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLATRISYGQSL GLAGSPAYVAPEVL+GKYSEKVDIWSAGVLLHALLVG LPFQGD
Sbjct: 241 ADFGLATRISYGQSLIGLAGSPAYVAPEVLSGKYSEKVDIWSAGVLLHALLVGALPFQGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLR 351
           SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDIS+RITAEEVLR
Sbjct: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISSRITAEEVLR 351

BLAST of Cucsat.G13525 vs. ExPASy TrEMBL
Match: A0A0A0LJM7 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G889970 PE=3 SV=1)

HSP 1 Score: 706 bits (1823), Expect = 6.41e-255
Identity = 351/351 (100.00%), Postives = 351/351 (100.00%), Query Frame = 0

Query: 1   MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK
Sbjct: 1   MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
           SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS
Sbjct: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL 180
           VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL
Sbjct: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL
Sbjct: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD
Sbjct: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLR 351
           SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLR
Sbjct: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLR 351

BLAST of Cucsat.G13525 vs. ExPASy TrEMBL
Match: A0A5D3E5W2 (Serine/threonine-protein kinase PEPKR2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005900 PE=3 SV=1)

HSP 1 Score: 692 bits (1787), Expect = 1.95e-249
Identity = 343/351 (97.72%), Postives = 348/351 (99.15%), Query Frame = 0

Query: 1   MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSETDGP NLQDNVTESH+SRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK
Sbjct: 1   MRKKRKGSETDGPSNLQDNVTESHDSRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
           SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSG+TIGHGKFGS
Sbjct: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGETIGHGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL 180
           VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYE+SECFHLVMEL
Sbjct: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEESECFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           C GGRLV+QMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL
Sbjct: 181 CRGGRLVDQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKY+EKVDIWSAGVLLHALLVG LPFQGD
Sbjct: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYTEKVDIWSAGVLLHALLVGALPFQGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLR 351
           SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLR
Sbjct: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLR 351

BLAST of Cucsat.G13525 vs. ExPASy TrEMBL
Match: A0A1S3CQM3 (serine/threonine-protein kinase PEPKR2 OS=Cucumis melo OX=3656 GN=LOC103503677 PE=3 SV=1)

HSP 1 Score: 692 bits (1787), Expect = 1.95e-249
Identity = 343/351 (97.72%), Postives = 348/351 (99.15%), Query Frame = 0

Query: 1   MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSETDGP NLQDNVTESH+SRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK
Sbjct: 1   MRKKRKGSETDGPSNLQDNVTESHDSRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
           SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSG+TIGHGKFGS
Sbjct: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGETIGHGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL 180
           VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYE+SECFHLVMEL
Sbjct: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEESECFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           C GGRLV+QMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL
Sbjct: 181 CRGGRLVDQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKY+EKVDIWSAGVLLHALLVG LPFQGD
Sbjct: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYTEKVDIWSAGVLLHALLVGALPFQGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLR 351
           SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLR
Sbjct: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLR 351

BLAST of Cucsat.G13525 vs. ExPASy TrEMBL
Match: A0A6J1IGJ4 (serine/threonine-protein kinase PEPKR2 OS=Cucurbita maxima OX=3661 GN=LOC111476741 PE=3 SV=1)

HSP 1 Score: 679 bits (1753), Expect = 2.93e-244
Identity = 337/351 (96.01%), Postives = 345/351 (98.29%), Query Frame = 0

Query: 1   MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSETDGPLNL+DNVTESH+SRSSKLKAHYSLEDYTRLNKRCKEDVGIEPV SCK
Sbjct: 1   MRKKRKGSETDGPLNLRDNVTESHDSRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVPSCK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
           SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSG+TIGHGKFGS
Sbjct: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGETIGHGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL 180
           VWLCKCKVSGAEYACKTLKK EETVHREVEIMQHLSGYPGVVTLLAVYE+SECFHLVMEL
Sbjct: 121 VWLCKCKVSGAEYACKTLKKAEETVHREVEIMQHLSGYPGVVTLLAVYEESECFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           C GGRLV+QM SEG+YSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL
Sbjct: 181 CRGGRLVDQMVSEGRYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLATRISYGQSL GLAGSPAYVAPEVL+GKYSEKVDIWSAGVLLHALLVG LPFQGD
Sbjct: 241 ADFGLATRISYGQSLIGLAGSPAYVAPEVLSGKYSEKVDIWSAGVLLHALLVGALPFQGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLR 351
           SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDIS+RITAEEVLR
Sbjct: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISSRITAEEVLR 351

BLAST of Cucsat.G13525 vs. ExPASy TrEMBL
Match: A0A6J1F9Y1 (serine/threonine-protein kinase PEPKR2 OS=Cucurbita moschata OX=3662 GN=LOC111443442 PE=3 SV=1)

HSP 1 Score: 676 bits (1745), Expect = 4.84e-243
Identity = 335/351 (95.44%), Postives = 345/351 (98.29%), Query Frame = 0

Query: 1   MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSETDGPLNL+DNVTESH+SRSSKLKAHYSLEDYTRLNKRCKEDVGIEPV SCK
Sbjct: 1   MRKKRKGSETDGPLNLRDNVTESHDSRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVPSCK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
           SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIE+DYVSG+TIGHGKFGS
Sbjct: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIEHDYVSGETIGHGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL 180
           VWLCKCKVSGAEYACKTLKK EETVHREVEIMQHLSGYPGVVTLLAVYE+SECFHLVMEL
Sbjct: 121 VWLCKCKVSGAEYACKTLKKAEETVHREVEIMQHLSGYPGVVTLLAVYEESECFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           C GGRLV+QM SEG+YSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTT+GKIKL
Sbjct: 181 CRGGRLVDQMVSEGRYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTAGKIKL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLATRISYGQSL GLAGSPAYVAPEVL+GKYSEKVDIWSAGVLLHALLVG LPFQGD
Sbjct: 241 ADFGLATRISYGQSLIGLAGSPAYVAPEVLSGKYSEKVDIWSAGVLLHALLVGALPFQGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLR 351
           SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDIS+RITAEEVLR
Sbjct: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISSRITAEEVLR 351

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8W4902.4e-14572.08Serine/threonine-protein kinase PEPKR2 OS=Arabidopsis thaliana OX=3702 GN=PEPKR2... [more]
Q6F3A67.8e-6442.36Calcium-dependent protein kinase 10 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Q9ZV151.2e-6142.07Calcium-dependent protein kinase 20 OS=Arabidopsis thaliana OX=3702 GN=CPK20 PE=... [more]
P536842.8e-6143.98Calcium-dependent protein kinase 7 OS=Oryza sativa subsp. japonica OX=39947 GN=C... [more]
P491016.2e-6143.28Calcium-dependent protein kinase 2 OS=Zea mays OX=4577 GN=CPK2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_031738766.11.32e-254100.00serine/threonine-protein kinase PEPKR2 [Cucumis sativus] >KGN60241.1 hypothetica... [more]
XP_008466187.14.02e-24997.72PREDICTED: serine/threonine-protein kinase PEPKR2 [Cucumis melo] >KAA0038682.1 s... [more]
XP_038899232.11.49e-24496.01serine/threonine-protein kinase PEPKR2 [Benincasa hispida] >XP_038899233.1 serin... [more]
XP_022976306.16.05e-24496.01serine/threonine-protein kinase PEPKR2 [Cucurbita maxima][more]
XP_023535980.16.05e-24496.01serine/threonine-protein kinase PEPKR2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A0A0LJM76.41e-255100.00Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G889... [more]
A0A5D3E5W21.95e-24997.72Serine/threonine-protein kinase PEPKR2 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3CQM31.95e-24997.72serine/threonine-protein kinase PEPKR2 OS=Cucumis melo OX=3656 GN=LOC103503677 P... [more]
A0A6J1IGJ42.93e-24496.01serine/threonine-protein kinase PEPKR2 OS=Cucurbita maxima OX=3661 GN=LOC1114767... [more]
A0A6J1F9Y14.84e-24395.44serine/threonine-protein kinase PEPKR2 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 107..300
e-value: 1.7E-29
score: 114.0
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 107..262
e-value: 6.8E-40
score: 137.1
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 107..310
score: 29.799431
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 98..263
e-value: 5.7E-48
score: 165.7
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 143..249
e-value: 9.7E-4
score: 16.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..28
NoneNo IPR availablePANTHERPTHR24349SERINE/THREONINE-PROTEIN KINASEcoord: 24..262
NoneNo IPR availablePANTHERPTHR24349:SF261SERINE/THREONINE-PROTEIN KINASE PEPKR2coord: 24..262
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 220..232
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 113..136
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 101..262

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G13525.T1Cucsat.G13525.T1mRNA
Cucsat.G13525.T3Cucsat.G13525.T3mRNA
Cucsat.G13525.T6Cucsat.G13525.T6mRNA
Cucsat.G13525.T2Cucsat.G13525.T2mRNA
Cucsat.G13525.T4Cucsat.G13525.T4mRNA
Cucsat.G13525.T8Cucsat.G13525.T8mRNA
Cucsat.G13525.T5Cucsat.G13525.T5mRNA
Cucsat.G13525.T7Cucsat.G13525.T7mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity