Cucsat.G13525.T1 (mRNA) Cucumber (B10) v3

Overview


Name	Cucsat.G13525.T1
Type	mRNA
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Protein kinase domain-containing protein
Location	ctg1838: 8304540 .. 8308556 (+)
RNA-Seq Expression	Cucsat.G13525.T1
Synteny	Cucsat.G13525.T1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTCGGGTCGTCGGGGTCGGGTCGGAAAGGGGCAAAAGCGGTAGAGTCGTGGTGATGTGCAGTGGAGTACGGAGTGGACACGTTGGTTGGACATAATAAAGTTTTTGGAGACCAAAAGAAAACCCCTAAATTGCTTTAGATTTTTATTAAATTTAATTTTGTTTTCCTATTTTTCTCTTGGGGTTGGGGGGATATTTTGGCTGTTTCCATATATTTTTTCTATTGTCGTTTATAAATCGGGGGGATTGCTGGATTTTCTCCCAAAGGGTAATGTGATCGAAGATTGCTAGGGTTTCGGATTCTGTGTTCTTCATCGTCTATTTCTTTAATGGATCGATCTTTGTGTTAATGGTGGTACTGGTTGTTTTGTTTAAGGTCCTCAGAACCGGCGATTTGATATGCGAGTGGTGGTGAAAGATATCTGGGGCTTTGAAGGTGCAATTTTATCATCTGGGTCTTTCTTGTTTTCTTTTGAAGTTTATGGAATTTGACGATGTAGATCTTTATATGAACTGGGGTTTGGGTTGAGCTTACGACTAATTGATTATGATATTTTGTTTGTTTTGGGGTATGTAATGTTAGTTACGATATGCAATCATTGATCTGTTTGCATTTTTTAAACGTGAGTTGAGAATCGGTGAATTTCTTGCAGTTTTCAGTTGTACGAAATCGGGGGTAAGAGCTGCTGGCTGATTAATTTGATTTGATGAGGAAGAAAAGGAAAGGAAGTGAGACTGATGGACCGTTAAATTTACAAGACAATGTGACAGAATCTCATGAATCGCGATCTTCTAAACTGAAGGCTCATTACTCGCTAGAAGACTATACGAGGTTGAATAAAAGGTGCAAGGAAGATGTGGGTATTGAACCGGTTCTTTCTTGTAAGAGTAGATTTGCAGGTATAGCTACAGCTCCACCCAGTGGGAGTTCGTCTCTGATTCTTCCTGGTAGAGGTTTGAAGAGGAAGATCGGATGCATAGACGTGGCTACTCAAATCGGGCGGAAACACAACATTGAGAATGATTATGTTTCAGGTAAGACTATTGGGCATGGAAAATTTGGCTCAGTTTGGCTATGTAAATGTAAGGTTAGTGGAGCTGAGTATGCTTGCAAGACCTTGAAAAAAGGGGAAGAAACTGTACATAGGGAGGTGGAGATAATGCAGCACTTATCTGGCTACCCTGGTGTTGTGACATTGTTGGCAGTGTACGAGGACTCCGAATGTTTTCACCTGGTTATGGAACTGTGTGGAGGAGGCCGTTTGGTCGAACAGATGGGCAGTGAAGGCCAATATTCAGAGCATCGAGCAGCCAATATACTAAAGGAAGTGATGTTGGTTATAAAATATTGTCATGATATGGGGGTTGTACATAGAGACATAAAGCCTGAAAATATTCTTCTCACAACATCTGGAAAGATAAAGCTTGCAGATTTTGGCCTGGCCACTAGGATTTCATATGGTATGCATTCTTTAATCTGTAATAGAGAGTACATCTTTGTATGTTTCTCTTCAAGTATTAGTTGCCTGATCTTCTTTGTAGTCTTCAATTGCAGACATCCAATGTTTATTGATCACCAGCTATGGAAGTGTTCACATTTTTTTGTTTCTTTTGTTACCTTTGCTTGTATCTTTCTGTGACCGTTCAAATGTCTTGTAAAGAAATAAGTGAGGAATGTCACTACAACTTAGTAGTATTTGAAGCACTGTAAATACAGCACTACAAAGTTATTAGATGAAATATGCTCAACAAACAAAGAAAGAAAATTTACGATCAAACTAAAGAAGTTGCTTAAAATTCCAATTAGCAAAAATAGATGTTGAACTATAGTCATAAAAAAAAAAGTTGTTAATTTTACTCCTTGCGTCACCCAATCCTGTTAGTCTATGGTTCCTTGGATAACCATTGTTCAGATTCAAGGTATGCTTATGCATTACAAGAATGATTGGACCGTAGCTCACATTTGGTGTTTAGCGACAATGCGTTAGCTTAGAGTGAGAGGATGCTTGTAAGGAGAGAAATTACTTTGGGAGTAAATTTTTTTTTGAGGGCCAAATGCTATGCTTGTAGAGAGATCAGATGTTGAGAGGGTGGGCTAAGCTTTATGAGCAGAACATCCATTTCAAATTGGACCCCTATGCTTTCACAACTTTGATGTATTGTTATTAAGATCACGTTTCCAAACGCTGGCCTAAACAGATTATTGTTTCCAACAATATATTCCAAAAACAAGTTTGTCAAGTGAGTTTAGTGTATTTAGGAGCATTTTTAGAAAACATAAAATATAAACTGATTAGGTACCACAATCCTGTGAGGTAAGACTTAATTGAATGGACAATGATATCGTAGATTGCAGCATTACATAAATTTTACACCATAATAGTTTTCTATTATATATTATGTTTCTTGCTTCTACTTTCTTGTAATGATGCCACTTGTATATTTCTCATTGTTGCTATCAACGGTGTGCAAATTTTGATGGTCTTTTACATTTAGAATAAGAGTTCCAAACATCTACTTATTTAAGTTATTCGACTGCGTTCTGCAGGCCAGAGTTTAACTGGTTTGGCTGGAAGCCCAGCTTATGTAGCCCCTGAAGTGTTGACAGGAAAGTACTCTGAGAAGGTGGATATTTGGAGTGCCGGTGTGCTCCTCCATGCTCTTTTGGTTGGCACTCTTCCTTTCCAAGGTGACTCATTAGAATCAGTTTTCGAAGCAATAAAGAACTCTAAGCTTGACTTCCACTCAGGGATGTGGGAGTCCATATCTAAACCTGCCCGCGATCTCATTGGGAGAATGCTGACAAGAGATATTTCAGCTAGGATAACAGCAGAAGAAGTCTTACGTAAGTTTGTTTTACTAATTGGTTTCATGCCCTGATGGTTAAGTAATACATAATCAGTTTTATATGCTATCGATATTTTGATATTCTGAAACAAGCATAAACCATTTGCTATGTTGTTATAAAGTCCAATATCCTTAAATAGATTGATGCCAATCTTAATCTTTTTAGTGTAATCTGATTTGGATCCAAAATGGTGATATTTCAGGACATCCCTGGATATTGTTTTATACCGAACGCACGTTGAAGGCTCTCCCGATCAAACTAAAATTGAAGAATCAAGAATCATCCCAACAGTCACCCATTGCAGCCAAAATTAAGTCTGACAGAAACAGGATTGATAGTGCAGCTAATATGACCTCCCTTAACGAAGTCTCTAACCTTTCCTCATCCGAGAGTTGCAATGCTGATGGTGATGATGTCGACGATTGCTTTCTGGTGGATGCACTTGCTACAGCTATTTCACATGTGAGAATTTCCGAGCCAAAGAGAAGTAGACTATGTGTTCCTACAGGACCTATCGAGCAGCACAGCTCCTCTAACATGAAAGCTAACAATCTTTGTAAAGCGTTTTGATCCTGCTGACAGGCCAACAACGACATCGTCAATACTGTTCTCTCCTGTTTTTTTTTACCTGAGCTGGGGGAACTACTACTTACAGGTAACACAACCCGGATGAATTCCTCCAAGTAAGCTAAAGAAGGTGATTTCCAGGCTAACATCAATGAAATCAATCCACAATATGATTTTTTCTTTTTTAATATGCAGTATGTGTTAGTCCATGCCTGAGGTGCATTAAATCTGATTAGTAGTGTGTGATTTTGTGCTTTAAGTCTCTAATAATTGTCTTAACAAGAACTGACAAATTTGATGTACATATTCTTGTAAATACAGCAGTAATGAAGAGTGCATTTTGATATTTTCGGTCTCCAATGCCCAAATTGGCTGCAGGAACCACACAGCAAATCTAAAGCCGTTGAGGCTCAATTTCTACTTCCTCTATATCTGTTATCTCAATTGAAAGTTTAGGTTTTACATTATTACTAATAGGTCACGTTTTCAAATCGTAATGAAAATGGGTGTATTTGGATTGAGATTCTATTGAATTATTGATGGGTAATGAAAATGGGTGTATTTGGATTGAGATTCTATTGGATTATTGATGGGTAAATCGCA

Coding sequence (CDS)

ATGAGGAAGAAAAGGAAAGGAAGTGAGACTGATGGACCGTTAAATTTACAAGACAATGTGACAGAATCTCATGAATCGCGATCTTCTAAACTGAAGGCTCATTACTCGCTAGAAGACTATACGAGGTTGAATAAAAGGTGCAAGGAAGATGTGGGTATTGAACCGGTTCTTTCTTGTAAGAGTAGATTTGCAGGTATAGCTACAGCTCCACCCAGTGGGAGTTCGTCTCTGATTCTTCCTGGTAGAGGTTTGAAGAGGAAGATCGGATGCATAGACGTGGCTACTCAAATCGGGCGGAAACACAACATTGAGAATGATTATGTTTCAGGTAAGACTATTGGGCATGGAAAATTTGGCTCAGTTTGGCTATGTAAATGTAAGGTTAGTGGAGCTGAGTATGCTTGCAAGACCTTGAAAAAAGGGGAAGAAACTGTACATAGGGAGGTGGAGATAATGCAGCACTTATCTGGCTACCCTGGTGTTGTGACATTGTTGGCAGTGTACGAGGACTCCGAATGTTTTCACCTGGTTATGGAACTGTGTGGAGGAGGCCGTTTGGTCGAACAGATGGGCAGTGAAGGCCAATATTCAGAGCATCGAGCAGCCAATATACTAAAGGAAGTGATGTTGGTTATAAAATATTGTCATGATATGGGGGTTGTACATAGAGACATAAAGCCTGAAAATATTCTTCTCACAACATCTGGAAAGATAAAGCTTGCAGATTTTGGCCTGGCCACTAGGATTTCATATGGCCAGAGTTTAACTGGTTTGGCTGGAAGCCCAGCTTATGTAGCCCCTGAAGTGTTGACAGGAAAGTACTCTGAGAAGGTGGATATTTGGAGTGCCGGTGTGCTCCTCCATGCTCTTTTGGTTGGCACTCTTCCTTTCCAAGGTGACTCATTAGAATCAGTTTTCGAAGCAATAAAGAACTCTAAGCTTGACTTCCACTCAGGGATGTGGGAGTCCATATCTAAACCTGCCCGCGATCTCATTGGGAGAATGCTGACAAGAGATATTTCAGCTAGGATAACAGCAGAAGAAGTCTTACGACATCCCTGGATATTGTTTTATACCGAACGCACGTTGAAGGCTCTCCCGATCAAACTAAAATTGAAGAATCAAGAATCATCCCAACAGTCACCCATTGCAGCCAAAATTAAGTCTGACAGAAACAGGATTGATAGTGCAGCTAATATGACCTCCCTTAACGAAGTCTCTAACCTTTCCTCATCCGAGAGTTGCAATGCTGATGGTGATGATGTCGACGATTGCTTTCTGGTGGATGCACTTGCTACAGCTATTTCACATGTGAGAATTTCCGAGCCAAAGAGAAGTAGACTATGTGTTCCTACAGGACCTATCGAGCAGCACAGCTCCTCTAACATGAAAGCTAACAATCTTTGTAAAGCGTTTTGA

Protein sequence

MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCKSRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGSVWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMELCGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKLADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGDSLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTERTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLNEVSNLSSSESCNADGDDVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF

Homology

[Close]

BLAST of Cucsat.G13525.T1 vs. ExPASy Swiss-Prot
Match: Q8W490 (Serine/threonine-protein kinase PEPKR2 OS=Arabidopsis thaliana OX=3702 GN=PEPKR2 PE=2 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 2.8e-170
Identity = 311/479 (64.93%), Postives = 379/479 (79.12%), Query Frame = 0


Query: 1   MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSET+G  NL D+++ S  SRSS  ++H+SLE Y RL KRCKE   +E V S K
Sbjct: 1   MRKKRKGSETEGWENLPDDLSCSTASRSSNFRSHFSLEGYARLKKRCKETEAVESVGSFK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
            R AG+ATAPP G+SSL+  GRGLKRKIGCIDV+TQ GRK+ I++DYV G+ IG GKFGS
Sbjct: 61  RRIAGVATAPPCGASSLVSSGRGLKRKIGCIDVSTQTGRKNKIDDDYVFGRNIGKGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL 180
           V +CK + +G E+ACKTLKKGEETVHREVEIMQHLSG+P VVTL AVYE+S+CFHLVMEL
Sbjct: 121 VRICKSRKNGTEFACKTLKKGEETVHREVEIMQHLSGHPRVVTLHAVYEESDCFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           C GGRL++QM   G+YSE RAANI K++MLVI YCH+MGVVHRDIKPENILLT +GKI+L
Sbjct: 181 CSGGRLIDQMVKVGRYSEQRAANIFKDLMLVINYCHEMGVVHRDIKPENILLTAAGKIQL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLA RI+ GQ+L+GLAGSPAYVAPEVL+  YSEKVD+WSAGVLL+ALL G LPF+GD
Sbjct: 241 ADFGLAMRIAKGQTLSGLAGSPAYVAPEVLSENYSEKVDVWSAGVLLYALLSGVLPFKGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360
           SL+++FEAIKN KLDF++G+WES+SKPARDL+ RMLTR+ SARITA+EVLRHPWILFYT+
Sbjct: 301 SLDAIFEAIKNVKLDFNTGVWESVSKPARDLLARMLTREESARITADEVLRHPWILFYTD 360

Query: 361 RTLKALPIKLKLKNQESS-----QQSPIAAKIKSDRNRIDSAANMTSLNEVSNLSSSESC 420
           RTLK + IK K K+Q  S      +SP     K+D NR D    + S +   + S++E  
Sbjct: 361 RTLKTMCIKSKHKSQAGSSTCLQNRSPTE---KTDLNRADREKKIPSDSPTDSFSNTE-- 420

Query: 421 NADGDDVDDCFLVDALATAISHVRISEPKRSRLCVPT-GPIEQHSSSNM-KANNLCKAF 473
               ++ D+  +VD L  AI++VRISEPKRSR+C PT  PIEQ  SSN+   + LC+AF
Sbjct: 421 ----EEEDESGVVDVLVVAIANVRISEPKRSRVCSPTNNPIEQQHSSNLTSTSTLCRAF 470

[Close]

BLAST of Cucsat.G13525.T1 vs. ExPASy Swiss-Prot
Match: Q6F3A6 (Calcium-dependent protein kinase 10 OS=Oryza sativa subsp. japonica OX=39947 GN=CPK10 PE=2 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 3.4e-67
Identity = 125/292 (42.81%), Postives = 185/292 (63.36%), Query Frame = 0


Query: 74  SSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGSVWLCKCKVSGAEY 133
           SS+ +L G  LKRK              ++++ Y  G+ +G G+FG+ +LC  + +G E+
Sbjct: 112 SSAGLLVGSVLKRK------------TESLKDKYSLGRKLGQGQFGTTYLCVERATGKEF 171

Query: 134 ACKTLKKGE-------ETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMELCGGGRL 193
           ACK++ K +       E V RE++IM HL+G+P V+++   YED+   HLVMELC GG L
Sbjct: 172 ACKSILKRKLVTDDDVEDVRREIQIMYHLAGHPNVISIRGAYEDAVAVHLVMELCAGGEL 231

Query: 194 VEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILL---TTSGKIKLADF 253
            +++  +G Y+E +AA + + ++ V++ CH MGV+HRD+KPEN L    T    +K  DF
Sbjct: 232 FDRIVQKGHYTERKAAELARVIVGVVEVCHSMGVMHRDLKPENFLFADQTEEAALKTIDF 291

Query: 254 GLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGDSLE 313
           GL+     GQ  T + GSP YVAPEVL  KY ++ D+WSAGV+++ LL G  PF  ++ +
Sbjct: 292 GLSIFFRPGQVFTDVVGSPYYVAPEVLKKKYGQEADVWSAGVIIYILLCGVPPFWAENEQ 351

Query: 314 SVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWI 356
            +FE + + +LDF S  W SIS+ A+DL+ RML RD   R+TA EVLRHPW+
Sbjct: 352 GIFEEVLHGRLDFQSEPWPSISEGAKDLVRRMLVRDPKKRLTAHEVLRHPWV 391

[Close]

BLAST of Cucsat.G13525.T1 vs. ExPASy Swiss-Prot
Match: P53684 (Calcium-dependent protein kinase 7 OS=Oryza sativa subsp. japonica OX=39947 GN=CPK7 PE=2 SV=2)

HSP 1 Score: 249.2 bits (635), Expect = 9.2e-65
Identity = 121/270 (44.81%), Postives = 171/270 (63.33%), Query Frame = 0


Query: 97  IGRK-HNIENDYVSGKTIGHGKFGSVWLCKCKVSGAEYACKTL-------KKGEETVHRE 156
           +GRK  ++   Y+ G+ +G G+FG+ +LC    +G EYACKT+       K+  E V RE
Sbjct: 68  LGRKTADLREHYIIGRKLGQGQFGTTYLCTEINTGCEYACKTIPKRKLITKEDVEDVRRE 127

Query: 157 VEIMQHLSGYPGVVTLLAVYEDSECFHLVMELCGGGRLVEQMGSEGQYSEHRAANILKEV 216
           ++IM HLSG+  VV +  VYED +  H+VMELC GG L +++  +G YSE +AA +++ +
Sbjct: 128 IQIMHHLSGHKNVVAIKDVYEDGQAVHIVMELCAGGELFDRIQEKGHYSERKAAELIRII 187

Query: 217 MLVIKYCHDMGVVHRDIKPENILLTTSG---KIKLADFGLATRISYGQSLTGLAGSPAYV 276
           + ++  CH +GV+HRD+KPEN LL        IK  DFGL+     GQ  T L GSP YV
Sbjct: 188 VSIVAMCHSLGVMHRDLKPENFLLLDKDDDLSIKAIDFGLSVFFKPGQVFTELVGSPYYV 247

Query: 277 APEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGDSLESVFEAIKNSKLDFHSGMWESIS 336
           APEVL  +Y  + D+WSAGV+L+ LL G  PF  ++ + +F+A+    +DF S  W  IS
Sbjct: 248 APEVLHKRYGPESDVWSAGVILYVLLSGVPPFWAETQQGIFDAVLKGHIDFQSDPWPKIS 307

Query: 337 KPARDLIGRMLTRDISARITAEEVLRHPWI 356
             A+DLI +ML+   S R+ A EVLRHPWI
Sbjct: 308 DSAKDLIRKMLSHCPSERLKAHEVLRHPWI 337

[Close]

BLAST of Cucsat.G13525.T1 vs. ExPASy Swiss-Prot
Match: Q9ZV15 (Calcium-dependent protein kinase 20 OS=Arabidopsis thaliana OX=3702 GN=CPK20 PE=3 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 2.7e-64
Identity = 117/275 (42.55%), Postives = 177/275 (64.36%), Query Frame = 0


Query: 91  IDVATQIGRK-HNIENDYVSGKTIGHGKFGSVWLCKCKVSGAEYACKTLKKGE------- 150
           + + + +GRK  N+++ Y  G+ +G G+FG+ +LC  K +G E+ACKT+ K +       
Sbjct: 117 LQIDSVLGRKTENLKDIYSVGRKLGQGQFGTTFLCVDKKTGKEFACKTIAKRKLTTPEDV 176

Query: 151 ETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMELCGGGRLVEQMGSEGQYSEHRAA 210
           E V RE++IM HLSG+P V+ ++  YED+   H+VME+C GG L +++   G Y+E +AA
Sbjct: 177 EDVRREIQIMHHLSGHPNVIQIVGAYEDAVAVHVVMEICAGGELFDRIIQRGHYTEKKAA 236

Query: 211 NILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGK---IKLADFGLATRISYGQSLTGLA 270
            + + ++ VI+ CH +GV+HRD+KPEN L  +  +   +K  DFGL+     G++ T + 
Sbjct: 237 ELARIIVGVIEACHSLGVMHRDLKPENFLFVSGDEEAALKTIDFGLSVFFKPGETFTDVV 296

Query: 271 GSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGDSLESVFEAIKNSKLDFHSG 330
           GSP YVAPEVL   YS + D+WSAGV+++ LL G  PF  ++ + +FE +    LDF S 
Sbjct: 297 GSPYYVAPEVLRKHYSHECDVWSAGVIIYILLSGVPPFWDETEQGIFEQVLKGDLDFISE 356

Query: 331 MWESISKPARDLIGRMLTRDISARITAEEVLRHPW 355
            W S+S+ A+DL+ RML RD   R+T  EVL HPW
Sbjct: 357 PWPSVSESAKDLVRRMLIRDPKKRMTTHEVLCHPW 391

[Close]

BLAST of Cucsat.G13525.T1 vs. ExPASy Swiss-Prot
Match: P28583 (Calcium-dependent protein kinase SK5 OS=Glycine max OX=3847 PE=1 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 2.7e-64
Identity = 123/268 (45.90%), Postives = 168/268 (62.69%), Query Frame = 0


Query: 99  RKHNIENDYVSGKTIGHGKFGSVWLCKCKVSGAEYACKTL-------KKGEETVHREVEI 158
           R  NI   Y  G+ +G G+FG+ + C  + SG ++ACK++       K+  E V RE++I
Sbjct: 26  RTQNIREVYEVGRKLGQGQFGTTFECTRRASGGKFACKSIPKRKLLCKEDYEDVWREIQI 85

Query: 159 MQHLSGYPGVVTLLAVYEDSECFHLVMELCGGGRLVEQMGSEGQYSEHRAANILKEVMLV 218
           M HLS +  VV +   YEDS   HLVMELC GG L +++  +G YSE +AA ++K ++ V
Sbjct: 86  MHHLSEHANVVRIEGTYEDSTAVHLVMELCEGGELFDRIVQKGHYSERQAARLIKTIVEV 145

Query: 219 IKYCHDMGVVHRDIKPENILLTT---SGKIKLADFGLATRISYGQSLTGLAGSPAYVAPE 278
           ++ CH +GV+HRD+KPEN L  T     K+K  DFGL+     G+S   + GSP YVAPE
Sbjct: 146 VEACHSLGVMHRDLKPENFLFDTIDEDAKLKATDFGLSVFYKPGESFCDVVGSPYYVAPE 205

Query: 279 VLTGKYSEKVDIWSAGVLLHALLVGTLPFQGDSLESVFEAIKNSKLDFHSGMWESISKPA 338
           VL   Y  + D+WSAGV+L+ LL G  PF  +S   +F  I   KLDFHS  W SIS  A
Sbjct: 206 VLRKLYGPESDVWSAGVILYILLSGVPPFWAESEPGIFRQILLGKLDFHSEPWPSISDSA 265

Query: 339 RDLIGRMLTRDISARITAEEVLRHPWIL 357
           +DLI +ML ++   R+TA EVLRHPWI+
Sbjct: 266 KDLIRKMLDQNPKTRLTAHEVLRHPWIV 293

[Close]

BLAST of Cucsat.G13525.T1 vs. NCBI nr
Match: XP_031738766.1 (serine/threonine-protein kinase PEPKR2 [Cucumis sativus] >KGN60241.1 hypothetical protein Csa_002436 [Cucumis sativus])

HSP 1 Score: 939 bits (2427), Expect = 0.0
Identity = 472/472 (100.00%), Postives = 472/472 (100.00%), Query Frame = 0


Query: 1   MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK
Sbjct: 1   MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
           SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS
Sbjct: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL 180
           VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL
Sbjct: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL
Sbjct: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD
Sbjct: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360
           SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE
Sbjct: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360

Query: 361 RTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLNEVSNLSSSESCNADGD 420
           RTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLNEVSNLSSSESCNADGD
Sbjct: 361 RTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLNEVSNLSSSESCNADGD 420

Query: 421 DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472
           DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF
Sbjct: 421 DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472

[Close]

BLAST of Cucsat.G13525.T1 vs. NCBI nr
Match: XP_008466187.1 (PREDICTED: serine/threonine-protein kinase PEPKR2 [Cucumis melo] >KAA0038682.1 serine/threonine-protein kinase PEPKR2 [Cucumis melo var. makuwa] >TYK31292.1 serine/threonine-protein kinase PEPKR2 [Cucumis melo var. makuwa])

HSP 1 Score: 922 bits (2383), Expect = 0.0
Identity = 461/472 (97.67%), Postives = 469/472 (99.36%), Query Frame = 0


Query: 1   MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSETDGP NLQDNVTESH+SRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK
Sbjct: 1   MRKKRKGSETDGPSNLQDNVTESHDSRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
           SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSG+TIGHGKFGS
Sbjct: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGETIGHGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL 180
           VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYE+SECFHLVMEL
Sbjct: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEESECFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           C GGRLV+QMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL
Sbjct: 181 CRGGRLVDQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKY+EKVDIWSAGVLLHALLVG LPFQGD
Sbjct: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYTEKVDIWSAGVLLHALLVGALPFQGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360
           SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE
Sbjct: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360

Query: 361 RTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLNEVSNLSSSESCNADGD 420
           RTLKALPIKLKLKNQESSQQSPIA+KIKSDRNRIDSAAN+TSLNE+SNLSSSESCNADGD
Sbjct: 361 RTLKALPIKLKLKNQESSQQSPIASKIKSDRNRIDSAANVTSLNEISNLSSSESCNADGD 420

Query: 421 DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472
           DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF
Sbjct: 421 DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472

[Close]

BLAST of Cucsat.G13525.T1 vs. NCBI nr
Match: XP_038899232.1 (serine/threonine-protein kinase PEPKR2 [Benincasa hispida] >XP_038899233.1 serine/threonine-protein kinase PEPKR2 [Benincasa hispida])

HSP 1 Score: 896 bits (2315), Expect = 0.0
Identity = 450/472 (95.34%), Postives = 459/472 (97.25%), Query Frame = 0


Query: 1   MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSETDGP NL DNVTESH+SRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK
Sbjct: 1   MRKKRKGSETDGPSNLPDNVTESHDSRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
           SRFAGIATAPPSGSSSLILPGRGLKRKIGCID+ATQIGRKHNIENDYVSG+TIGHGKFGS
Sbjct: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDMATQIGRKHNIENDYVSGETIGHGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL 180
           VWLCKCK SG EYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYE++ECFHLVMEL
Sbjct: 121 VWLCKCKASGTEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEEAECFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           C GGRLV+QM SEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL
Sbjct: 181 CRGGRLVDQMVSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLATRISYGQSLTGLAGSPAYVAPEVL+GKYSEKVDIWSAGVLLHALLVG LPFQGD
Sbjct: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLSGKYSEKVDIWSAGVLLHALLVGALPFQGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360
           SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE
Sbjct: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360

Query: 361 RTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLNEVSNLSSSESCNADGD 420
           RTLK LPIKLKLKNQES  QSPIAAKIK DR RIDSAAN+TSLNEVSNLSSSESCNAD D
Sbjct: 361 RTLKTLPIKLKLKNQESCLQSPIAAKIKCDRIRIDSAANVTSLNEVSNLSSSESCNADVD 420

Query: 421 DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472
           DVDDCFLVDALATAISHVRISEPKRSR+CVPTGPIEQHSSSNMKANNLCKAF
Sbjct: 421 DVDDCFLVDALATAISHVRISEPKRSRVCVPTGPIEQHSSSNMKANNLCKAF 472

[Close]

BLAST of Cucsat.G13525.T1 vs. NCBI nr
Match: XP_023535980.1 (serine/threonine-protein kinase PEPKR2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 895 bits (2313), Expect = 0.0
Identity = 449/472 (95.13%), Postives = 461/472 (97.67%), Query Frame = 0


Query: 1   MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSETDGPLNL+DNVTESH+SRSSKLKAHYSLEDYTRLNKRCKEDVGIEPV SCK
Sbjct: 1   MRKKRKGSETDGPLNLRDNVTESHDSRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVPSCK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
           SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSG+TIGHGKFGS
Sbjct: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGETIGHGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL 180
           VWLCKCKVSGAEYACKTLKK EETVHREVEIMQHLSGYPGVVTLLAVYE+SECFHLVMEL
Sbjct: 121 VWLCKCKVSGAEYACKTLKKAEETVHREVEIMQHLSGYPGVVTLLAVYEESECFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           C GGRLV+QM SEG+YSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL
Sbjct: 181 CRGGRLVDQMVSEGRYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLATRISYGQSL GLAGSPAYVAPEVL+GKYSEKVDIWSAGVLLHALLVG LPFQGD
Sbjct: 241 ADFGLATRISYGQSLIGLAGSPAYVAPEVLSGKYSEKVDIWSAGVLLHALLVGALPFQGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360
           SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDIS+RITAEEVLRHPWILFYTE
Sbjct: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISSRITAEEVLRHPWILFYTE 360

Query: 361 RTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLNEVSNLSSSESCNADGD 420
           RTLK LPIKLKLKNQES QQSPI+AK KSDRNRIDSAAN TSLNEVS+LSSSESCNA+G+
Sbjct: 361 RTLKTLPIKLKLKNQESCQQSPISAKTKSDRNRIDSAANSTSLNEVSDLSSSESCNANGE 420

Query: 421 DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472
           D DDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF
Sbjct: 421 DDDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472

[Close]

BLAST of Cucsat.G13525.T1 vs. NCBI nr
Match: XP_022976306.1 (serine/threonine-protein kinase PEPKR2 [Cucurbita maxima])

HSP 1 Score: 892 bits (2306), Expect = 0.0
Identity = 447/472 (94.70%), Postives = 461/472 (97.67%), Query Frame = 0


Query: 1   MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSETDGPLNL+DNVTESH+SRSSKLKAHYSLEDYTRLNKRCKEDVGIEPV SCK
Sbjct: 1   MRKKRKGSETDGPLNLRDNVTESHDSRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVPSCK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
           SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSG+TIGHGKFGS
Sbjct: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGETIGHGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL 180
           VWLCKCKVSGAEYACKTLKK EETVHREVEIMQHLSGYPGVVTLLAVYE+SECFHLVMEL
Sbjct: 121 VWLCKCKVSGAEYACKTLKKAEETVHREVEIMQHLSGYPGVVTLLAVYEESECFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           C GGRLV+QM SEG+YSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL
Sbjct: 181 CRGGRLVDQMVSEGRYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLATRISYGQSL GLAGSPAYVAPEVL+GKYSEKVDIWSAGVLLHALLVG LPFQGD
Sbjct: 241 ADFGLATRISYGQSLIGLAGSPAYVAPEVLSGKYSEKVDIWSAGVLLHALLVGALPFQGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360
           SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDIS+RITAEEVLRHPWILFYTE
Sbjct: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISSRITAEEVLRHPWILFYTE 360

Query: 361 RTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLNEVSNLSSSESCNADGD 420
           RTLK LPIKLKLKNQES Q SPI+AK KSDRNRIDSAAN+TSLNEVS+LSSSESCNA+G+
Sbjct: 361 RTLKTLPIKLKLKNQESCQPSPISAKTKSDRNRIDSAANLTSLNEVSDLSSSESCNANGE 420

Query: 421 DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472
           D +DCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF
Sbjct: 421 DDEDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472

[Close]

BLAST of Cucsat.G13525.T1 vs. ExPASy TrEMBL
Match: A0A0A0LJM7 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G889970 PE=3 SV=1)

HSP 1 Score: 939 bits (2427), Expect = 0.0
Identity = 472/472 (100.00%), Postives = 472/472 (100.00%), Query Frame = 0


Query: 1   MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK
Sbjct: 1   MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
           SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS
Sbjct: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL 180
           VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL
Sbjct: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL
Sbjct: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD
Sbjct: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360
           SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE
Sbjct: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360

Query: 361 RTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLNEVSNLSSSESCNADGD 420
           RTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLNEVSNLSSSESCNADGD
Sbjct: 361 RTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLNEVSNLSSSESCNADGD 420

Query: 421 DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472
           DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF
Sbjct: 421 DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472

[Close]

BLAST of Cucsat.G13525.T1 vs. ExPASy TrEMBL
Match: A0A5D3E5W2 (Serine/threonine-protein kinase PEPKR2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005900 PE=3 SV=1)

HSP 1 Score: 922 bits (2383), Expect = 0.0
Identity = 461/472 (97.67%), Postives = 469/472 (99.36%), Query Frame = 0


Query: 1   MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSETDGP NLQDNVTESH+SRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK
Sbjct: 1   MRKKRKGSETDGPSNLQDNVTESHDSRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
           SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSG+TIGHGKFGS
Sbjct: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGETIGHGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL 180
           VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYE+SECFHLVMEL
Sbjct: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEESECFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           C GGRLV+QMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL
Sbjct: 181 CRGGRLVDQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKY+EKVDIWSAGVLLHALLVG LPFQGD
Sbjct: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYTEKVDIWSAGVLLHALLVGALPFQGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360
           SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE
Sbjct: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360

Query: 361 RTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLNEVSNLSSSESCNADGD 420
           RTLKALPIKLKLKNQESSQQSPIA+KIKSDRNRIDSAAN+TSLNE+SNLSSSESCNADGD
Sbjct: 361 RTLKALPIKLKLKNQESSQQSPIASKIKSDRNRIDSAANVTSLNEISNLSSSESCNADGD 420

Query: 421 DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472
           DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF
Sbjct: 421 DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472

[Close]

BLAST of Cucsat.G13525.T1 vs. ExPASy TrEMBL
Match: A0A1S3CQM3 (serine/threonine-protein kinase PEPKR2 OS=Cucumis melo OX=3656 GN=LOC103503677 PE=3 SV=1)

HSP 1 Score: 922 bits (2383), Expect = 0.0
Identity = 461/472 (97.67%), Postives = 469/472 (99.36%), Query Frame = 0


Query: 1   MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSETDGP NLQDNVTESH+SRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK
Sbjct: 1   MRKKRKGSETDGPSNLQDNVTESHDSRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
           SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSG+TIGHGKFGS
Sbjct: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGETIGHGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL 180
           VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYE+SECFHLVMEL
Sbjct: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEESECFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           C GGRLV+QMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL
Sbjct: 181 CRGGRLVDQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKY+EKVDIWSAGVLLHALLVG LPFQGD
Sbjct: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYTEKVDIWSAGVLLHALLVGALPFQGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360
           SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE
Sbjct: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360

Query: 361 RTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLNEVSNLSSSESCNADGD 420
           RTLKALPIKLKLKNQESSQQSPIA+KIKSDRNRIDSAAN+TSLNE+SNLSSSESCNADGD
Sbjct: 361 RTLKALPIKLKLKNQESSQQSPIASKIKSDRNRIDSAANVTSLNEISNLSSSESCNADGD 420

Query: 421 DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472
           DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF
Sbjct: 421 DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472

[Close]

BLAST of Cucsat.G13525.T1 vs. ExPASy TrEMBL
Match: A0A6J1IGJ4 (serine/threonine-protein kinase PEPKR2 OS=Cucurbita maxima OX=3661 GN=LOC111476741 PE=3 SV=1)

HSP 1 Score: 892 bits (2306), Expect = 0.0
Identity = 447/472 (94.70%), Postives = 461/472 (97.67%), Query Frame = 0


Query: 1   MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSETDGPLNL+DNVTESH+SRSSKLKAHYSLEDYTRLNKRCKEDVGIEPV SCK
Sbjct: 1   MRKKRKGSETDGPLNLRDNVTESHDSRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVPSCK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
           SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSG+TIGHGKFGS
Sbjct: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGETIGHGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL 180
           VWLCKCKVSGAEYACKTLKK EETVHREVEIMQHLSGYPGVVTLLAVYE+SECFHLVMEL
Sbjct: 121 VWLCKCKVSGAEYACKTLKKAEETVHREVEIMQHLSGYPGVVTLLAVYEESECFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           C GGRLV+QM SEG+YSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL
Sbjct: 181 CRGGRLVDQMVSEGRYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLATRISYGQSL GLAGSPAYVAPEVL+GKYSEKVDIWSAGVLLHALLVG LPFQGD
Sbjct: 241 ADFGLATRISYGQSLIGLAGSPAYVAPEVLSGKYSEKVDIWSAGVLLHALLVGALPFQGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360
           SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDIS+RITAEEVLRHPWILFYTE
Sbjct: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISSRITAEEVLRHPWILFYTE 360

Query: 361 RTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLNEVSNLSSSESCNADGD 420
           RTLK LPIKLKLKNQES Q SPI+AK KSDRNRIDSAAN+TSLNEVS+LSSSESCNA+G+
Sbjct: 361 RTLKTLPIKLKLKNQESCQPSPISAKTKSDRNRIDSAANLTSLNEVSDLSSSESCNANGE 420

Query: 421 DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472
           D +DCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF
Sbjct: 421 DDEDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472

[Close]

BLAST of Cucsat.G13525.T1 vs. ExPASy TrEMBL
Match: A0A6J1F9Y1 (serine/threonine-protein kinase PEPKR2 OS=Cucurbita moschata OX=3662 GN=LOC111443442 PE=3 SV=1)

HSP 1 Score: 890 bits (2299), Expect = 0.0
Identity = 446/472 (94.49%), Postives = 460/472 (97.46%), Query Frame = 0


Query: 1   MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSETDGPLNL+DNVTESH+SRSSKLKAHYSLEDYTRLNKRCKEDVGIEPV SCK
Sbjct: 1   MRKKRKGSETDGPLNLRDNVTESHDSRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVPSCK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
           SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIE+DYVSG+TIGHGKFGS
Sbjct: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIEHDYVSGETIGHGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL 180
           VWLCKCKVSGAEYACKTLKK EETVHREVEIMQHLSGYPGVVTLLAVYE+SECFHLVMEL
Sbjct: 121 VWLCKCKVSGAEYACKTLKKAEETVHREVEIMQHLSGYPGVVTLLAVYEESECFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           C GGRLV+QM SEG+YSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTT+GKIKL
Sbjct: 181 CRGGRLVDQMVSEGRYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTAGKIKL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLATRISYGQSL GLAGSPAYVAPEVL+GKYSEKVDIWSAGVLLHALLVG LPFQGD
Sbjct: 241 ADFGLATRISYGQSLIGLAGSPAYVAPEVLSGKYSEKVDIWSAGVLLHALLVGALPFQGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360
           SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDIS+RITAEEVLRHPWILFYTE
Sbjct: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISSRITAEEVLRHPWILFYTE 360

Query: 361 RTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLNEVSNLSSSESCNADGD 420
           RTLK LPIKLKLKNQES Q SPI+AK KSDRNRIDSAAN TSLNEVS+LSSSESCNA+G+
Sbjct: 361 RTLKTLPIKLKLKNQESCQPSPISAKTKSDRNRIDSAANSTSLNEVSDLSSSESCNANGE 420

Query: 421 DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472
           D DDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF
Sbjct: 421 DDDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472

The following BLAST results are available for this feature:

BLAST of Cucsat.G13525.T1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8W490	2.8e-170	64.93	Serine/threonine-protein kinase PEPKR2 OS=Arabidopsis thaliana OX=3702 GN=PEPKR2...	[more]
Q6F3A6	3.4e-67	42.81	Calcium-dependent protein kinase 10 OS=Oryza sativa subsp. japonica OX=39947 GN=...	[more]
P53684	9.2e-65	44.81	Calcium-dependent protein kinase 7 OS=Oryza sativa subsp. japonica OX=39947 GN=C...	[more]
Q9ZV15	2.7e-64	42.55	Calcium-dependent protein kinase 20 OS=Arabidopsis thaliana OX=3702 GN=CPK20 PE=...	[more]
P28583	2.7e-64	45.90	Calcium-dependent protein kinase SK5 OS=Glycine max OX=3847 PE=1 SV=1	[more]

BLAST of Cucsat.G13525.T1 vs. NCBI nr


Match Name	Identity	Description
XP_031738766.1	100.00	serine/threonine-protein kinase PEPKR2 [Cucumis sativus] >KGN60241.1 hypothetica...	[more]
XP_008466187.1	97.67	PREDICTED: serine/threonine-protein kinase PEPKR2 [Cucumis melo] >KAA0038682.1 s...	[more]
XP_038899232.1	95.34	serine/threonine-protein kinase PEPKR2 [Benincasa hispida] >XP_038899233.1 serin...	[more]
XP_023535980.1	95.13	serine/threonine-protein kinase PEPKR2 [Cucurbita pepo subsp. pepo]	[more]
XP_022976306.1	94.70	serine/threonine-protein kinase PEPKR2 [Cucurbita maxima]	[more]

BLAST of Cucsat.G13525.T1 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A0A0LJM7	100.00	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G889...	[more]
A0A5D3E5W2	97.67	Serine/threonine-protein kinase PEPKR2 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3CQM3	97.67	serine/threonine-protein kinase PEPKR2 OS=Cucumis melo OX=3656 GN=LOC103503677 P...	[more]
A0A6J1IGJ4	94.70	serine/threonine-protein kinase PEPKR2 OS=Cucurbita maxima OX=3661 GN=LOC1114767...	[more]
A0A6J1F9Y1	94.49	serine/threonine-protein kinase PEPKR2 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 107..355 e-value: 6.4E-95 score: 331.3
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 107..355 e-value: 2.9E-68 score: 230.1
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 107..355 score: 49.163013
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 98..419 e-value: 1.6E-86 score: 292.5
None	No IPR available	PIRSR	PIRSR000620-2	PIRSR000620-2	coord: 143..309 e-value: 1.2E-8 score: 32.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..28
None	No IPR available	PANTHER	PTHR24349:SF261	SERINE/THREONINE-PROTEIN KINASE PEPKR2	coord: 24..464
None	No IPR available	PANTHER	PTHR24349	SERINE/THREONINE-PROTEIN KINASE	coord: 24..464
None	No IPR available	CDD	cd05117	STKc_CAMK	coord: 106..354 e-value: 2.15343E-115 score: 337.911
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 220..232
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 113..136
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 100..379

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsat.G13525	Cucsat.G13525	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G13525.T1.E1	Cucsat.G13525.T1.E1	exon
Cucsat.G13525.T1.E2	Cucsat.G13525.T1.E2	exon
Cucsat.G13525.T1.E3	Cucsat.G13525.T1.E3	exon
Cucsat.G13525.T1.E4	Cucsat.G13525.T1.E4	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsat.G13525.T1.C1	Cucsat.G13525.T1.C1	CDS
Cucsat.G13525.T1.C2	Cucsat.G13525.T1.C2	CDS
Cucsat.G13525.T1.C3	Cucsat.G13525.T1.C3	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsat.G13525.T1	Cucsat.G13525.T1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity
molecular_function	GO:0004674	protein serine/threonine kinase activity