Chy12G209430 (gene) Cucumber (hystrix) v1

Overview


Name	Chy12G209430
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	PseudoU_synth_1 domain-containing protein
Location	chrH12: 3614575 .. 3616878 (+)
RNA-Seq Expression	Chy12G209430
Synteny	Chy12G209430

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAATCCCTGAAAGTGCATGGATAGACGACCCTTGTGGTATTTCACTTGCTGATTACATCAACTCTTATCTTCCTGATAATATCAACGTTTTTGGCATTGTACCTGCACAGAAGTGAGTGTTCTGAGGTTTTTTCATTGGTGCTTTTTAAGTGAGTAAATGAAGTGTTAGCACCAGCATGATACACTTTTACGCATAAACTACAAAGTGGTAACATTGCATCAATTTTGTTACTTGTCAAGGAGGTTTGATCCTAGAAAGGAGTGTGATGTTCGGAAGTATTCATATCTCCTCCCTGCTAAGCTTATTGGAATTGAAAGTAAACTTGAAACTACTGAAATTGATTGCCACATCTCAGACTTTAAAGATATATTAAATTCCTTTGAGGTACGTTATGATCTCCTTCCATTAATTACATACGCATCGTATTGCCCCTTCCTTCACTGCAAGAAACATGATTTGTTATTTGATACAGTATAGTGCTGTTATTTGGGAAGACAATTATATGTCGTTGAAGAACAAAGGTGAATTCTTTTTGGCCACAGGGGGACCATCCTTTTCATAATTATACAATACGCTCCAATTATAGAACGAGAATTAGAGCCAAGCGACTAGTATATGCCCTTAAGTCAGGCAAGAAGGTATCACCCGGTGATATGTCAAACTCTGAGTTTGAGGGAAGTGATCAAGAAAATGATACTTTTGTTGAAGGAGTCAAGGAACCCGATCAACGTTCTCCAAGCTTAAGCGTCTGTGAAAATCTAACTGAAGTATGCAGTAAACTTGAAGATGGATCAGAACTTCTGAAGGATGTTCGTGCAAGATGGCTTCATGAGCCAGATGAGAAAGATCGTATAGGCGCCTCACACTTCAGAAGGATTCTTTACTGTTCTTGTGGGAAGCTGGAAACATACATTGGATATGATTTCATTGAGATATCCATTTGGGGAGAATCGTTCATGTTGCATCAAGTTAGTCTTTCTCTAACATTGTTTTTATGGTACATAATTTGTTCTCATCCATTTTCATTCCAATTTGTAAGTTAATCTATATTTACTCCATTTATTCTTAATTTCAGTTGGTAGCTCTATTGCTTAGATAGGTTTGCCGTTGTAATGCCTCTGGTAGCTTATATCTTGCTTCTGGCCATGTGGGAGCCAAAATTTGGTTTTATAACTGTACTTTGGTGCCCACTCAACAACTTTTTTGCTGCATTATTTCACTTTTTAAACTCTTTTCCCTCTTTGGAAATCAATACTGGCTTAATGGGCTTTTTCCTGTGCTAGTAGTCCAAAAAATTAAGAAATAGCTATGGGTTTATGTTTTAGGAAATTAAATTAAATTCTAAAATATTACCATCGATTAAATAATTTGATGGTCCTACCCATTCCTGGACGAAAGATAGTAGTCAATGGGTTCAAGTTTTATGAGCTTGACTTGTTCAATATCTAACATTATAACCTTCATTTACTGCCTGAGGTTCTAAATTTGTAGGATCAGCATGGTCCCTGTGCATTGCTGGACAGAAGATATGAGTTGATGAGTTCAAGCTTTATGAGCTTGACTTGTTAAATATATACCATCATAACCTTCATTTACTCCCTAAGGTTCATAATTCATGAGTTCAAGCTTTATGAGAAACTAGAATCTATTATTAAGTTTAGGAATTGGAAGGAGAAATGGGAGGTGAAAAGGTGTCAGGCTTTCTTTTGGCAGATTTCTCATTTTCATTCTCTTTGTAACTTTTGAGCTGATAATATGATGTTTTCCTATTAAAAAAGAACATCAATAACATATCTTATGGACAATGAAGTTGTTTTCATTAGTAATTAATAAAGGGTAGTTAATGTTCTAGTTTTAAAACGAAAGGGCCATTCTGATGCAACTTTTATTACATCCTTGGCAGATTCGGAAAATGATAGCAACGGCTGTGGCAGTGAAGCGGAAACTTCTCCCAAGAGATATTATAGCATTGTCACTGACCAAGTTTTCTAGAATAGTTTTACCCATTGCTCCAGCTGAGGTCTTAGTCCTAAGAGGGAACAGTTTTTCCAACAGAAAACGGCCAGGAGCTCCAATAAGGTCAGAGTTGTTCACACTCGATGAATCAGATGACATAGTCAAGTCAGTGAATGAATTCTACAGATCAGCGATATTGCCCCAGGTAACTAAGTTCCTGGACACAGAAAGCCCCCCATGGAAAGAATGGGTGGAGAAATTGGACAAGTACACAAGCATTCCGAATGAAGAATTGGATGAAGTTAGGAGTGCTTGGAAAGCATGGAAGAACAGGGAACATTGA

mRNA sequence

ATGGAAATCCCTGAAAGTGCATGGATAGACGACCCTTGTGGTATTTCACTTGCTGATTACATCAACTCTTATCTTCCTGATAATATCAACGTTTTTGGCATTGTACCTGCACAGAAGAGGTTTGATCCTAGAAAGGAGTGTGATGTTCGGAAGTATTCATATCTCCTCCCTGCTAAGCTTATTGGAATTGAAAGTAAACTTGAAACTACTGAAATTGATTGCCACATCTCAGACTTTAAAGATATATTAAATTCCTTTGAGGGGGACCATCCTTTTCATAATTATACAATACGCTCCAATTATAGAACGAGAATTAGAGCCAAGCGACTAGTATATGCCCTTAAGTCAGGCAAGAAGGTATCACCCGGTGATATGTCAAACTCTGAGTTTGAGGGAAGTGATCAAGAAAATGATACTTTTGTTGAAGGAGTCAAGGAACCCGATCAACGTTCTCCAAGCTTAAGCGTCTGTGAAAATCTAACTGAAGTATGCAGTAAACTTGAAGATGGATCAGAACTTCTGAAGGATGTTCGTGCAAGATGGCTTCATGAGCCAGATGAGAAAGATCGTATAGGCGCCTCACACTTCAGAAGGATTCTTTACTGTTCTTGTGGGAAGCTGGAAACATACATTGGATATGATTTCATTGAGATATCCATTTGGGGAGAATCGTTCATGTTGCATCAAATTCGGAAAATGATAGCAACGGCTGTGGCAGTGAAGCGGAAACTTCTCCCAAGAGATATTATAGCATTGTCACTGACCAAGTTTTCTAGAATAGTTTTACCCATTGCTCCAGCTGAGGTCTTAGTCCTAAGAGGGAACAGTTTTTCCAACAGAAAACGGCCAGGAGCTCCAATAAGGTCAGAGTTGTTCACACTCGATGAATCAGATGACATAGTCAAGTCAGTGAATGAATTCTACAGATCAGCGATATTGCCCCAGGTAACTAAGTTCCTGGACACAGAAAGCCCCCCATGGAAAGAATGGGTGGAGAAATTGGACAAGTACACAAGCATTCCGAATGAAGAATTGGATGAAGTTAGGAGTGCTTGGAAAGCATGGAAGAACAGGGAACATTGA

Coding sequence (CDS)

ATGGAAATCCCTGAAAGTGCATGGATAGACGACCCTTGTGGTATTTCACTTGCTGATTACATCAACTCTTATCTTCCTGATAATATCAACGTTTTTGGCATTGTACCTGCACAGAAGAGGTTTGATCCTAGAAAGGAGTGTGATGTTCGGAAGTATTCATATCTCCTCCCTGCTAAGCTTATTGGAATTGAAAGTAAACTTGAAACTACTGAAATTGATTGCCACATCTCAGACTTTAAAGATATATTAAATTCCTTTGAGGGGGACCATCCTTTTCATAATTATACAATACGCTCCAATTATAGAACGAGAATTAGAGCCAAGCGACTAGTATATGCCCTTAAGTCAGGCAAGAAGGTATCACCCGGTGATATGTCAAACTCTGAGTTTGAGGGAAGTGATCAAGAAAATGATACTTTTGTTGAAGGAGTCAAGGAACCCGATCAACGTTCTCCAAGCTTAAGCGTCTGTGAAAATCTAACTGAAGTATGCAGTAAACTTGAAGATGGATCAGAACTTCTGAAGGATGTTCGTGCAAGATGGCTTCATGAGCCAGATGAGAAAGATCGTATAGGCGCCTCACACTTCAGAAGGATTCTTTACTGTTCTTGTGGGAAGCTGGAAACATACATTGGATATGATTTCATTGAGATATCCATTTGGGGAGAATCGTTCATGTTGCATCAAATTCGGAAAATGATAGCAACGGCTGTGGCAGTGAAGCGGAAACTTCTCCCAAGAGATATTATAGCATTGTCACTGACCAAGTTTTCTAGAATAGTTTTACCCATTGCTCCAGCTGAGGTCTTAGTCCTAAGAGGGAACAGTTTTTCCAACAGAAAACGGCCAGGAGCTCCAATAAGGTCAGAGTTGTTCACACTCGATGAATCAGATGACATAGTCAAGTCAGTGAATGAATTCTACAGATCAGCGATATTGCCCCAGGTAACTAAGTTCCTGGACACAGAAAGCCCCCCATGGAAAGAATGGGTGGAGAAATTGGACAAGTACACAAGCATTCCGAATGAAGAATTGGATGAAGTTAGGAGTGCTTGGAAAGCATGGAAGAACAGGGAACATTGA

Protein sequence

MEIPESAWIDDPCGISLADYINSYLPDNINVFGIVPAQKRFDPRKECDVRKYSYLLPAKLIGIESKLETTEIDCHISDFKDILNSFEGDHPFHNYTIRSNYRTRIRAKRLVYALKSGKKVSPGDMSNSEFEGSDQENDTFVEGVKEPDQRSPSLSVCENLTEVCSKLEDGSELLKDVRARWLHEPDEKDRIGASHFRRILYCSCGKLETYIGYDFIEISIWGESFMLHQIRKMIATAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPIRSELFTLDESDDIVKSVNEFYRSAILPQVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKNREH*

Homology

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BLAST of Chy12G209430 vs. ExPASy Swiss-Prot
Match: O22928 (Putative tRNA pseudouridine synthase OS=Arabidopsis thaliana OX=3702 GN=At2g30320 PE=3 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 2.6e-96
Identity = 176/356 (49.44%), Postives = 243/356 (68.26%), Query Frame = 0


Query: 1   MEIPESAWIDDPCGISLADYINSYLPDNINVFGIVPAQKRFDPRKECDVRKYSYLLPAKL 60
           MEIPE+AW DDP G  LA  I+ +LP+NI VF ++P+ +RFDPR+EC +RKYSYLLP  +
Sbjct: 139 MEIPETAWKDDPQGTVLAKCISKHLPENIRVFSVLPSNRRFDPRRECTLRKYSYLLPVDV 198

Query: 61  IGIESKLETTEIDCHISDFKDILNSFEGDHPFHNYTIRSNYRTRIRAKRLVYALKSGKKV 120
           +GI++   + EID HI+DF +IL  FEG++PFHNYT RS YR +   K     +K     
Sbjct: 199 LGIKNSFTSDEIDYHITDFNEILKEFEGEYPFHNYTQRSRYRRKSEQK-----IKQRNGR 258

Query: 121 SPGDMSNSEFEGSDQENDTFVEGVKEPDQRSPSLSVCENLTEVCSKLEDGSELLKDVRAR 180
            P +  + +   S+   +  +E  +E +++       E+   V +   D S++    RA+
Sbjct: 259 PPREPKSIKASESEFREENHIEIGEEEEEKEVD---GESDEHVVTPDSDNSQVYS--RAK 318

Query: 181 WLHEPDEKDRIGASHFRRILYCSCGKLETYIGYDFIEISIWGESFMLHQIRKMIATAVAV 240
           WL+EPDE D+I  +HFR++  C  GKLE  +G+ F+EISIWGESFMLHQIRKMI TAVAV
Sbjct: 319 WLYEPDETDKISGAHFRKVFRCRSGKLENSLGFGFVEISIWGESFMLHQIRKMIGTAVAV 378

Query: 241 KRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPIRSELFTLDESDDI 300
           KR+LLPRDII LSL KF+RIVLP+AP+EVL+LRGNSF  R+ P  P    +    ES+++
Sbjct: 379 KRELLPRDIIRLSLNKFTRIVLPLAPSEVLILRGNSFEVRRLPERP---GMEATGESEEV 438

Query: 301 VKSVNEFYRSAILPQVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWK 357
            K + EFYR+ ++PQV+ FLD+E  PWKEWV+ LD+   + +EEL++VR  W+ WK
Sbjct: 439 EKEIEEFYRAVMVPQVSIFLDSEKSPWKEWVDHLDRNDGLIDEELEDVRKGWEEWK 481

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BLAST of Chy12G209430 vs. ExPASy Swiss-Prot
Match: O94295 (tRNA pseudouridine synthase 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=pus2 PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 2.5e-09
Identity = 73/285 (25.61%), Postives = 128/285 (44.91%), Query Frame = 0


Query: 8   WIDDPCGIS-LADYINSYLPDNINVFGIVPAQKRFDPRKECDVRKYSYLLP--AKLIGIE 67
           ++D P  IS L   +N  L   I V+ + PA K F+PR  C+ R Y YL+P  A L    
Sbjct: 112 FVDKPLDISLLKTELNEALCSQIRVWSVFPAPKYFNPRISCESRTYEYLIPSFALLPPKP 171

Query: 68  SKLETTEIDCHISDFKDILNSFEGDHPFHNYTIRSNYRT-RIRAKRLVYALKSGKKVSPG 127
           S     ++  ++S  + + N  E +  +    + S + T +++ K +     + K     
Sbjct: 172 SCPLFKKMQKNLS--RKLDNELERNLVYSMNDLISFWNTVKLKQKEIQEMFDTNK----- 231

Query: 128 DMSNSEFEGSDQENDTFVEGVKEPDQR--SPSLSVCENLTEVCSKLEDG-----SELLKD 187
           D   + F+G   E      G+  P Q     +L   E    +  ++++       +LLK 
Sbjct: 232 DAFTNPFKGMFYEK-PIPAGIVIPPQAKLKKALKQAEYYCYMNYRIKEDRLKVLQQLLKK 291

Query: 188 VRAR--WLHEPDEKDRIGASHFRRILYCSCGKLETYIGYDFIEISIWGESFMLHQIRKMI 247
              R  + +     D    S++R I   +CG    Y  +++I ++I G SFML+QIRKM+
Sbjct: 292 YEGRHNFHNFTVTDDSTSPSNYRFIESVTCGTPFVYENWEWIPVTIKGNSFMLNQIRKMM 351

Query: 248 ATAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSN 280
           A  + + R   P  +I  +      + +  +P  VL+L+   FS+
Sbjct: 352 AHVLMIIRSCAPTGLIDKAFDPNITMNISKSPGHVLLLKDIKFSS 388

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BLAST of Chy12G209430 vs. ExPASy Swiss-Prot
Match: P53167 (tRNA pseudouridine(27/28) synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PUS2 PE=1 SV=4)

HSP 1 Score: 63.2 bits (152), Expect = 7.1e-09
Identity = 76/283 (26.86%), Postives = 111/283 (39.22%), Query Frame = 0


Query: 21  INSYLPDNINVFGIVPAQKRFDPRKECDVRKYSYLLPA-KLIG--IESKLETTEIDCHIS 80
           +N+ LP  I V+GI P  K+F+ R  CD R Y YL+P   LIG    S L      C+  
Sbjct: 80  LNAALPPEIRVWGIQPVNKKFNARSACDSRWYQYLIPEFILIGPPRSSLLHRNVGGCYRE 139

Query: 81  DFKDILNSFEGDHPFHNYTIRSNYRTRIRAKRLVYALKSGKKVSPGDMSNSEFEGSDQEN 140
           D         G     + T     R R     L     + +K+S  D    ++ G     
Sbjct: 140 D---------GSQEVWD-TFLEQTRGRFSGDELCRLQDTAQKLSESDPLVQDYVG----- 199

Query: 141 DTFVEGVKEPDQRSPSLSVCENLTEVCSKLEDGSELLKDVRARWLHEPDEKDRIGASHFR 200
              + G       SPS     +  E   +   G+    +     L       R    H +
Sbjct: 200 --LLSGTLSGYCLSPSKL---DAFEAAMQEYVGTHNFHNFTTGKLWGDPSAQR----HIK 259

Query: 201 RILYCSCGKLETYIGYDFIEISIWGESFMLHQIRKMIATAVAVKRKLLPRDIIALSLTKF 260
           +++              +I + I G+SFMLHQIR+M+A AV   R  LP +I+       
Sbjct: 260 KVVVSQASP-------GWICVRIHGQSFMLHQIRRMVALAVLAARCQLPPNIVRNYFNAG 319

Query: 261 SRIVLPIAPAEVLVLRGNSFSNRKRPGAPIRSELFTLDESDDI 301
            R  +P APA+ L+L G  F         +R+ L+     DDI
Sbjct: 320 PRKYIPRAPAQGLLLEGPVFDGY---NTKLRNLLYCEIRPDDI 328

[Close]

BLAST of Chy12G209430 vs. ExPASy Swiss-Prot
Match: Q59S63 (tRNA pseudouridine synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=PUS1 PE=3 SV=2)

HSP 1 Score: 62.8 bits (151), Expect = 9.3e-09
Identity = 81/288 (28.12%), Postives = 124/288 (43.06%), Query Frame = 0


Query: 9   IDDPCGISLADYINSYLPDNINVFGIVPAQKRFDPRKECDVRKYSYLLPA-KLIGIESKL 68
           I+DP    + + IN  LP  I ++GI    K FD RK C  R Y YLLP   L+  + K 
Sbjct: 226 IEDP---EIINKINDLLPKQIRIWGIQRTTKGFDCRKCCSSRIYEYLLPTFSLLPPKPKS 285

Query: 69  ETTEIDCHISDFK-DILNSFEGDHPFHNYTIRSNYRTRIRAKRLVYALKSGKKVSPGDMS 128
             +E+   + + K +  + FE D    ++    N +++I        L SG      D  
Sbjct: 286 VLSEL---VKEKKLENPDLFEDDQEGIDWW--ENVKSKI--------LASGITQEQIDSI 345

Query: 129 NSEFEGSDQENDTFVEGVKEPDQRSPSLSVCENLTEVCSKLEDGSELLKDVRARWLHEPD 188
            S ++   Q+     +   + ++R  S      L +    +E+ S     + +  L    
Sbjct: 346 TSSYDEQQQQQQQQQQQQADEEERDISELSFTKLIKQIKTIENQSRRSYRISSSRLQHFR 405

Query: 189 E--KDRIGASHFRRILYCSCGK--------LETYI-------GYDFIEISIWGESFMLHQ 248
           E  K   G  +F         K        ++T +       G ++I I I G+SFMLHQ
Sbjct: 406 EVMKQYEGTHNFHNFTVGKPFKDTSANRFMIKTIVSDPFVIEGTEWISIKIHGQSFMLHQ 465

Query: 249 IRKMIATAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSF 278
           IRKMIA A  V R  LP  II  +  + ++I +P APA  L+L    F
Sbjct: 466 IRKMIAMAALVVRLSLPCGIIN-NFFQSTKINIPKAPALGLLLENPVF 496

[Close]

BLAST of Chy12G209430 vs. ExPASy Swiss-Prot
Match: Q6CC39 (tRNA pseudouridine synthase 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=PUS1 PE=3 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 2.2e-05
Identity = 63/270 (23.33%), Postives = 114/270 (42.22%), Query Frame = 0


Query: 16  SLADYINSYLPDNINVFGIVPAQKRFDPRKECDVRKYSYLLPAKLIGIESKLETTEIDCH 75
           ++ + INS+LP+ + V+G+    K F+ RK C  R Y YL+P     +  +  T      
Sbjct: 164 NIVEKINSHLPEQLRVWGVSRTNKAFECRKLCSSRVYEYLMPTYSF-LNPRPGT------ 223

Query: 76  ISDFKDILNSFEGDHPFHNYTIRSNYRTRIRAKRLVY-------ALKSGKKVSPGDMSNS 135
           +   K + +    D     Y    +    + ++ + Y        +   K     +++ S
Sbjct: 224 VMSEKLLKDGTSPDEEGKKYW--ESVAADLESQGVSYDEWMKRACIDEIKGEETKEVAES 283

Query: 136 EFEGSDQENDTFVEGVKEPDQRSP-----SLSVCENLTEVCSKLEDGSELLKDVRARWLH 195
           E +   + +   +E +K  ++R       S      + E+    E           +   
Sbjct: 284 EVKTDSKTDAATLEKIKAVERRHREEFRISGERLAKIREILKIYEGTHNFHNFTLGKAFK 343

Query: 196 EPDEKDRIGASHFRRILYCSCGKLETYIGYDFIEISIWGESFMLHQIRKMIA-TAVAVKR 255
           +P        S  R +   +C       G +++ I I G+SFMLHQIRKMI+  A++V+ 
Sbjct: 344 DP--------SAMRTMKSLTCSDPFLIDGTEWVSIKIHGQSFMLHQIRKMISMVALSVRC 403

Query: 256 KLLPRDIIALSLTKFSRIVLPIAPAEVLVL 273
              P+ +I  +  K +RI +P APA  L+L
Sbjct: 404 NADPQKLIPQTFEK-ARINIPKAPALGLLL 415

[Close]

BLAST of Chy12G209430 vs. ExPASy TrEMBL
Match: A0A0A0M0Z2 (PseudoU_synth_1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G533680 PE=3 SV=1)

HSP 1 Score: 698.0 bits (1800), Expect = 2.1e-197
Identity = 342/360 (95.00%), Postives = 349/360 (96.94%), Query Frame = 0


Query: 1   MEIPESAWIDDPCGISLADYINSYLPDNINVFGIVPAQKRFDPRKECDVRKYSYLLPAKL 60
           MEIP SAWIDDPCGISLADYINSYLPDNI VFGIVPAQKRFDPRKECDVR YSYLLPAKL
Sbjct: 62  MEIPGSAWIDDPCGISLADYINSYLPDNIKVFGIVPAQKRFDPRKECDVRMYSYLLPAKL 121

Query: 61  IGIESKLETTEIDCHISDFKDILNSFEGDHPFHNYTIRSNYRTRIRAKRLVYALKSGKKV 120
           IGIESKL+TTEIDCHISDFKDIL SFEGDHPFHNYT+RS YRTRIRAK+LVYALKSGKKV
Sbjct: 122 IGIESKLKTTEIDCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLVYALKSGKKV 181

Query: 121 SPGDMSNSEFEGSDQENDTFVEGVKEPDQRSPSLSVCENLTEVCSKLEDGSELLKDVRAR 180
           SPGDMSNSEFEGSDQENDTFVEG+KEPDQRSPSLSVCENLTEVCSKLEDGSELLK VRAR
Sbjct: 182 SPGDMSNSEFEGSDQENDTFVEGIKEPDQRSPSLSVCENLTEVCSKLEDGSELLKGVRAR 241

Query: 181 WLHEPDEKDRIGASHFRRILYCSCGKLETYIGYDFIEISIWGESFMLHQIRKMIATAVAV 240
           WLHEPDEKDRIGASHFRRILYCSCGKLETY G DFIEISIWGESFMLHQIRKMIATAVAV
Sbjct: 242 WLHEPDEKDRIGASHFRRILYCSCGKLETYFGCDFIEISIWGESFMLHQIRKMIATAVAV 301

Query: 241 KRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPIRSELFTLDESDDI 300
           KRKLLPRDII LSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAP+RSELFTL+ESDDI
Sbjct: 302 KRKLLPRDIITLSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPMRSELFTLNESDDI 361

Query: 301 VKSVNEFYRSAILPQVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKNREH 360
           V SVNEFYRSAILP+VTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWK REH
Sbjct: 362 VNSVNEFYRSAILPEVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKYREH 421

[Close]

BLAST of Chy12G209430 vs. ExPASy TrEMBL
Match: A0A1S3C5W1 (putative tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497029 PE=3 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 2.4e-185
Identity = 324/361 (89.75%), Postives = 339/361 (93.91%), Query Frame = 0


Query: 1   MEIPESAWIDDPCGISLADYINSYLPDNINVFGIVPAQKRFDPRKECDVRKYSYLLPAKL 60
           MEIPESAW+ DPCGISLADYINSYLPDNI VFGIVP+Q+ FDPRKECDVRKYSYLLPAKL
Sbjct: 148 MEIPESAWMGDPCGISLADYINSYLPDNIKVFGIVPSQRSFDPRKECDVRKYSYLLPAKL 207

Query: 61  IGIESKLETTEIDCHISDFKDILNSFEGDHPFHNYTIRSNYRTRIRAKRLV-YALKSGKK 120
           IGIESKL+TTEIDCHISDFKDILNSFEG+HPFHNYTIRSNYRTRIRAK+   YALKSG K
Sbjct: 208 IGIESKLKTTEIDCHISDFKDILNSFEGEHPFHNYTIRSNYRTRIRAKQSPGYALKSGNK 267

Query: 121 VSPGDMSNSEFEGSDQENDTFVEGVKEPDQRSPSLSVCENLTEVCSKLEDGSELLKDVRA 180
           VSPG+MSN EFE SD+E+DTFVEGVKEPDQ SPSLSVCENLTEVCSKLEDGSELLK VRA
Sbjct: 268 VSPGEMSNFEFEESDREDDTFVEGVKEPDQCSPSLSVCENLTEVCSKLEDGSELLKGVRA 327

Query: 181 RWLHEPDEKDRIGASHFRRILYCSCGKLETYIGYDFIEISIWGESFMLHQIRKMIATAVA 240
           RWLHEPDEKDRIGASHFRRIL CSCGKLET  GYDFIEISIWGESFMLHQIRKMI TAVA
Sbjct: 328 RWLHEPDEKDRIGASHFRRILCCSCGKLETSFGYDFIEISIWGESFMLHQIRKMIGTAVA 387

Query: 241 VKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPIRSELFTLDESDD 300
           VKRKLLPRD+IALSLTKFSRIVLPIAPAEVLVLRGNSFS RKRPG P R +L TL+ESDD
Sbjct: 388 VKRKLLPRDVIALSLTKFSRIVLPIAPAEVLVLRGNSFSTRKRPGVPTRPDLVTLNESDD 447

Query: 301 IVKSVNEFYRSAILPQVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKNRE 360
           I+KSVNEFYRSAILP+VT+FLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWK R 
Sbjct: 448 IIKSVNEFYRSAILPEVTEFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKYRV 507

[Close]

BLAST of Chy12G209430 vs. ExPASy TrEMBL
Match: A0A6J1HM83 (putative tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111464888 PE=3 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 8.4e-162
Identity = 286/360 (79.44%), Postives = 316/360 (87.78%), Query Frame = 0


Query: 1   MEIPESAWIDDPCGISLADYINSYLPDNINVFGIVPAQKRFDPRKECDVRKYSYLLPAKL 60
           MEIP+SAW+ DPCGI LA+YIN YLP+NI VFGIVP+Q+ FDPR+ECDVRKY+YLLPAKL
Sbjct: 148 MEIPDSAWMGDPCGILLANYINFYLPENIRVFGIVPSQRSFDPRRECDVRKYTYLLPAKL 207

Query: 61  IGIESKLETTEIDCHISDFKDILNSFEGDHPFHNYTIRSNYRTRIRAKRLV-YALKSGKK 120
           IGIESKL TTEID HIS+FKDILNSF+G+HPFHNYTIRS YRTRIR  +   Y  KSG  
Sbjct: 208 IGIESKLTTTEIDYHISEFKDILNSFQGEHPFHNYTIRSKYRTRIRTNQSPGYVHKSGDG 267

Query: 121 VSPGDMSNSEFEGSDQENDTFVEGVKEPDQRSPSLS-VCENLTEVCSKLEDGSELLKDVR 180
           VS  D+SNSEFE SD END FV G+KEP+Q SPSLS   EN  EVCSKL +GSEL + VR
Sbjct: 268 VSSSDLSNSEFEESDGENDIFVGGIKEPNQHSPSLSGSYENTFEVCSKLGNGSELPQGVR 327

Query: 181 ARWLHEPDEKDRIGASHFRRILYCSCGKLETYIGYDFIEISIWGESFMLHQIRKMIATAV 240
           ARWLHEPDEKDRIGASHFR+ILYCSCGKLET +GYDF+EISIWGESFMLHQIRKM+ TA+
Sbjct: 328 ARWLHEPDEKDRIGASHFRKILYCSCGKLETSLGYDFVEISIWGESFMLHQIRKMVGTAI 387

Query: 241 AVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPIRSELFTLDESD 300
           AVKRKLLPRDIIALSL+KFSRIVLPIAPAEVLVLRGN FSNRKRPGAP R +L TL+ES 
Sbjct: 388 AVKRKLLPRDIIALSLSKFSRIVLPIAPAEVLVLRGNRFSNRKRPGAPKRPDLLTLNESH 447

Query: 301 DIVKSVNEFYRSAILPQVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKNR 359
           DIVKSVNEFYRSAILP+VT FLD E PPWKEWVEKLDKYTSIPNE+L+EVR+AWKAW  +
Sbjct: 448 DIVKSVNEFYRSAILPEVTNFLDIERPPWKEWVEKLDKYTSIPNEQLEEVRNAWKAWTGK 507

[Close]

BLAST of Chy12G209430 vs. ExPASy TrEMBL
Match: A0A6J1I156 (putative tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111469569 PE=3 SV=1)

HSP 1 Score: 577.0 bits (1486), Expect = 5.4e-161
Identity = 286/361 (79.22%), Postives = 318/361 (88.09%), Query Frame = 0


Query: 1   MEIPESAWIDDPCGISLADYINSYLPDNINVFGIVPAQKRFDPRKECDVRKYSYLLPAKL 60
           MEIP+SAW+ DPCGI LA+YIN YLP+NI VFGIVP+Q+ FDPR+ECDVRKY+YLLPAKL
Sbjct: 148 MEIPDSAWMGDPCGILLANYINFYLPENIKVFGIVPSQRSFDPRRECDVRKYTYLLPAKL 207

Query: 61  IGIESKLETTEIDCHISDFKDILNSFEGDHPFHNYTIRSNYRTRIRAKRLV-YALKSGKK 120
           IGIESKL TTEID HIS+FKDILNSF+G+HPFHNYTIRS YRTRIRA +   Y  KSG  
Sbjct: 208 IGIESKLTTTEIDYHISEFKDILNSFQGEHPFHNYTIRSKYRTRIRANQSPGYVHKSGDG 267

Query: 121 VSPGDMSNSEFEGSDQENDTFV-EGVKEPDQRSPSLSVC-ENLTEVCSKLEDGSELLKDV 180
           VS  D+SNSEFE SD END FV  G+KEP+Q SPSLS C EN  EVCSKL +GSEL + V
Sbjct: 268 VSSSDLSNSEFEESDGENDIFVGGGIKEPNQHSPSLSGCNENTFEVCSKLGNGSELPQGV 327

Query: 181 RARWLHEPDEKDRIGASHFRRILYCSCGKLETYIGYDFIEISIWGESFMLHQIRKMIATA 240
           RARWLHEPDE+DRIGASHFR+IL CSCGKLET +GYDF+EISIWGESFMLHQIRKM+ TA
Sbjct: 328 RARWLHEPDERDRIGASHFRKILCCSCGKLETSLGYDFVEISIWGESFMLHQIRKMVGTA 387

Query: 241 VAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPIRSELFTLDES 300
           +AVKRKLLPRDIIALSL+KFSRIVLPIAPAEVLVLRGN FSNRKRPGAP R +L TL++S
Sbjct: 388 IAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVLVLRGNRFSNRKRPGAPKRPDLLTLNKS 447

Query: 301 DDIVKSVNEFYRSAILPQVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKN 359
           DDIVKSVNEFYRSAILP+VT FLD E PPWKEWVEKLDKYTSIPNE+L+EVR+AWKAW  
Sbjct: 448 DDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWVEKLDKYTSIPNEQLEEVRNAWKAWTG 507

[Close]

BLAST of Chy12G209430 vs. ExPASy TrEMBL
Match: A0A6J1DMV6 (putative tRNA pseudouridine synthase isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022025 PE=3 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 2.7e-160
Identity = 283/360 (78.61%), Postives = 316/360 (87.78%), Query Frame = 0


Query: 1   MEIPESAWIDDPCGISLADYINSYLPDNINVFGIVPAQKRFDPRKECDVRKYSYLLPAKL 60
           MEIPE+AW+ DPCGI LA+YIN YLPDNI VFGIVP+Q+ FDPR+ECDVRKYSYLLPAKL
Sbjct: 147 MEIPENAWMGDPCGILLANYINFYLPDNIKVFGIVPSQRSFDPRRECDVRKYSYLLPAKL 206

Query: 61  IGIESKLETTEIDCHISDFKDILNSFEGDHPFHNYTIRSNYRTRIRAKRLV-YALKSGKK 120
           IGIESK  TTEID HIS+FKDILNSF+G+HPFHNYTIRS YR  IRA++   Y   SG++
Sbjct: 207 IGIESKFNTTEIDHHISEFKDILNSFQGEHPFHNYTIRSKYRKTIRARQSPGYGHNSGEE 266

Query: 121 VSPGDMSNSEFEGSDQENDTFVEGVKEPDQRSPSLSV-CENLTEVCSKLEDGSELLKDVR 180
           VSP  MSNS FE SD E++ FVEGVK P+Q +P LSV CE+  EVCSKL +GSEL K VR
Sbjct: 267 VSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAPRLSVCCEDPVEVCSKLGNGSELQKGVR 326

Query: 181 ARWLHEPDEKDRIGASHFRRILYCSCGKLETYIGYDFIEISIWGESFMLHQIRKMIATAV 240
           ARWLHEPDEKDRIGASHFR+IL CSCGKLET +GYDF+EISIWGESFMLHQIRKM+ TAV
Sbjct: 327 ARWLHEPDEKDRIGASHFRKILCCSCGKLETSLGYDFVEISIWGESFMLHQIRKMVGTAV 386

Query: 241 AVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPIRSELFTLDESD 300
           AVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSN KRPGAP R +L TL++SD
Sbjct: 387 AVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNGKRPGAPKRPDLLTLNKSD 446

Query: 301 DIVKSVNEFYRSAILPQVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKNR 359
           DI+KSV+EFYRSAILP++T FLDTE  PW EWVEKLDKYTSIPNE+LDEVR+AWKAWK +
Sbjct: 447 DIIKSVDEFYRSAILPELTNFLDTEKAPWNEWVEKLDKYTSIPNEQLDEVRNAWKAWKEK 506

[Close]

BLAST of Chy12G209430 vs. NCBI nr
Match: XP_004145230.1 (putative tRNA pseudouridine synthase isoform X2 [Cucumis sativus] >KGN66289.2 hypothetical protein Csa_023335 [Cucumis sativus])

HSP 1 Score: 694 bits (1791), Expect = 3.56e-249
Identity = 342/360 (95.00%), Postives = 349/360 (96.94%), Query Frame = 0


Query: 1   MEIPESAWIDDPCGISLADYINSYLPDNINVFGIVPAQKRFDPRKECDVRKYSYLLPAKL 60
           MEIP SAWIDDPCGISLADYINSYLPDNI VFGIVPAQKRFDPRKECDVR YSYLLPAKL
Sbjct: 148 MEIPGSAWIDDPCGISLADYINSYLPDNIKVFGIVPAQKRFDPRKECDVRMYSYLLPAKL 207

Query: 61  IGIESKLETTEIDCHISDFKDILNSFEGDHPFHNYTIRSNYRTRIRAKRLVYALKSGKKV 120
           IGIESKL+TTEIDCHISDFKDIL SFEGDHPFHNYT+RS YRTRIRAK+LVYALKSGKKV
Sbjct: 208 IGIESKLKTTEIDCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLVYALKSGKKV 267

Query: 121 SPGDMSNSEFEGSDQENDTFVEGVKEPDQRSPSLSVCENLTEVCSKLEDGSELLKDVRAR 180
           SPGDMSNSEFEGSDQENDTFVEG+KEPDQRSPSLSVCENLTEVCSKLEDGSELLK VRAR
Sbjct: 268 SPGDMSNSEFEGSDQENDTFVEGIKEPDQRSPSLSVCENLTEVCSKLEDGSELLKGVRAR 327

Query: 181 WLHEPDEKDRIGASHFRRILYCSCGKLETYIGYDFIEISIWGESFMLHQIRKMIATAVAV 240
           WLHEPDEKDRIGASHFRRILYCSCGKLETY G DFIEISIWGESFMLHQIRKMIATAVAV
Sbjct: 328 WLHEPDEKDRIGASHFRRILYCSCGKLETYFGCDFIEISIWGESFMLHQIRKMIATAVAV 387

Query: 241 KRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPIRSELFTLDESDDI 300
           KRKLLPRDII LSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAP+RSELFTL+ESDDI
Sbjct: 388 KRKLLPRDIITLSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPMRSELFTLNESDDI 447

Query: 301 VKSVNEFYRSAILPQVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKNREH 360
           V SVNEFYRSAILP+VTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWK REH
Sbjct: 448 VNSVNEFYRSAILPEVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKYREH 507

[Close]

BLAST of Chy12G209430 vs. NCBI nr
Match: XP_031742713.1 (putative tRNA pseudouridine synthase isoform X1 [Cucumis sativus])

HSP 1 Score: 694 bits (1791), Expect = 7.10e-249
Identity = 342/360 (95.00%), Postives = 349/360 (96.94%), Query Frame = 0


Query: 1   MEIPESAWIDDPCGISLADYINSYLPDNINVFGIVPAQKRFDPRKECDVRKYSYLLPAKL 60
           MEIP SAWIDDPCGISLADYINSYLPDNI VFGIVPAQKRFDPRKECDVR YSYLLPAKL
Sbjct: 167 MEIPGSAWIDDPCGISLADYINSYLPDNIKVFGIVPAQKRFDPRKECDVRMYSYLLPAKL 226

Query: 61  IGIESKLETTEIDCHISDFKDILNSFEGDHPFHNYTIRSNYRTRIRAKRLVYALKSGKKV 120
           IGIESKL+TTEIDCHISDFKDIL SFEGDHPFHNYT+RS YRTRIRAK+LVYALKSGKKV
Sbjct: 227 IGIESKLKTTEIDCHISDFKDILKSFEGDHPFHNYTVRSIYRTRIRAKQLVYALKSGKKV 286

Query: 121 SPGDMSNSEFEGSDQENDTFVEGVKEPDQRSPSLSVCENLTEVCSKLEDGSELLKDVRAR 180
           SPGDMSNSEFEGSDQENDTFVEG+KEPDQRSPSLSVCENLTEVCSKLEDGSELLK VRAR
Sbjct: 287 SPGDMSNSEFEGSDQENDTFVEGIKEPDQRSPSLSVCENLTEVCSKLEDGSELLKGVRAR 346

Query: 181 WLHEPDEKDRIGASHFRRILYCSCGKLETYIGYDFIEISIWGESFMLHQIRKMIATAVAV 240
           WLHEPDEKDRIGASHFRRILYCSCGKLETY G DFIEISIWGESFMLHQIRKMIATAVAV
Sbjct: 347 WLHEPDEKDRIGASHFRRILYCSCGKLETYFGCDFIEISIWGESFMLHQIRKMIATAVAV 406

Query: 241 KRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPIRSELFTLDESDDI 300
           KRKLLPRDII LSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAP+RSELFTL+ESDDI
Sbjct: 407 KRKLLPRDIITLSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPMRSELFTLNESDDI 466

Query: 301 VKSVNEFYRSAILPQVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKNREH 360
           V SVNEFYRSAILP+VTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWK REH
Sbjct: 467 VNSVNEFYRSAILPEVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKYREH 526

[Close]

BLAST of Chy12G209430 vs. NCBI nr
Match: XP_008457308.1 (PREDICTED: putative tRNA pseudouridine synthase [Cucumis melo])

HSP 1 Score: 654 bits (1687), Expect = 2.49e-233
Identity = 324/361 (89.75%), Postives = 339/361 (93.91%), Query Frame = 0


Query: 1   MEIPESAWIDDPCGISLADYINSYLPDNINVFGIVPAQKRFDPRKECDVRKYSYLLPAKL 60
           MEIPESAW+ DPCGISLADYINSYLPDNI VFGIVP+Q+ FDPRKECDVRKYSYLLPAKL
Sbjct: 148 MEIPESAWMGDPCGISLADYINSYLPDNIKVFGIVPSQRSFDPRKECDVRKYSYLLPAKL 207

Query: 61  IGIESKLETTEIDCHISDFKDILNSFEGDHPFHNYTIRSNYRTRIRAKRLV-YALKSGKK 120
           IGIESKL+TTEIDCHISDFKDILNSFEG+HPFHNYTIRSNYRTRIRAK+   YALKSG K
Sbjct: 208 IGIESKLKTTEIDCHISDFKDILNSFEGEHPFHNYTIRSNYRTRIRAKQSPGYALKSGNK 267

Query: 121 VSPGDMSNSEFEGSDQENDTFVEGVKEPDQRSPSLSVCENLTEVCSKLEDGSELLKDVRA 180
           VSPG+MSN EFE SD+E+DTFVEGVKEPDQ SPSLSVCENLTEVCSKLEDGSELLK VRA
Sbjct: 268 VSPGEMSNFEFEESDREDDTFVEGVKEPDQCSPSLSVCENLTEVCSKLEDGSELLKGVRA 327

Query: 181 RWLHEPDEKDRIGASHFRRILYCSCGKLETYIGYDFIEISIWGESFMLHQIRKMIATAVA 240
           RWLHEPDEKDRIGASHFRRIL CSCGKLET  GYDFIEISIWGESFMLHQIRKMI TAVA
Sbjct: 328 RWLHEPDEKDRIGASHFRRILCCSCGKLETSFGYDFIEISIWGESFMLHQIRKMIGTAVA 387

Query: 241 VKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPIRSELFTLDESDD 300
           VKRKLLPRD+IALSLTKFSRIVLPIAPAEVLVLRGNSFS RKRPG P R +L TL+ESDD
Sbjct: 388 VKRKLLPRDVIALSLTKFSRIVLPIAPAEVLVLRGNSFSTRKRPGVPTRPDLVTLNESDD 447

Query: 301 IVKSVNEFYRSAILPQVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKNRE 360
           I+KSVNEFYRSAILP+VT+FLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWK R 
Sbjct: 448 IIKSVNEFYRSAILPEVTEFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKYRV 507

[Close]

BLAST of Chy12G209430 vs. NCBI nr
Match: XP_038894775.1 (putative tRNA pseudouridine synthase isoform X2 [Benincasa hispida])

HSP 1 Score: 606 bits (1563), Expect = 8.22e-216
Identity = 302/361 (83.66%), Postives = 326/361 (90.30%), Query Frame = 0


Query: 1   MEIPESAWIDDPCGISLADYINSYLPDNINVFGIVPAQKRFDPRKECDVRKYSYLLPAKL 60
           MEIPESAW+ DPCGISLA+YIN YLPDNI VFGIVP+QK FDPRKECDVRKYSY+LPAKL
Sbjct: 65  MEIPESAWMGDPCGISLANYINFYLPDNIKVFGIVPSQKSFDPRKECDVRKYSYVLPAKL 124

Query: 61  IGIESKLETTEIDCHISDFKDILNSFEGDHPFHNYTIRSNYRTRIRAKRLVYALKSGKKV 120
           IGI +KL+TTE+D HISDFKDILNSF+G+HPFHNYTIRS YRTRIRAKR   +   G +V
Sbjct: 125 IGIGNKLKTTEVDYHISDFKDILNSFQGEHPFHNYTIRSKYRTRIRAKR---SPGYGHEV 184

Query: 121 SPGDMSNSEFEGSDQENDTFVEGVKEPDQRSPSLSVC-ENLTEVCSKLEDGSELLKDVRA 180
           SP D+SNSEFE SD E+DTFVEGVKEPDQ SPSLSVC EN TEVCSKL DGSEL KDVRA
Sbjct: 185 SPSDVSNSEFEESDGEDDTFVEGVKEPDQGSPSLSVCYENPTEVCSKLGDGSELPKDVRA 244

Query: 181 RWLHEPDEKDRIGASHFRRILYCSCGKLETYIGYDFIEISIWGESFMLHQIRKMIATAVA 240
           RWLHEPDEKDRIGASHFR+IL CSCGKLET  GYDF+EISIWGESFMLHQIRKMI TA+A
Sbjct: 245 RWLHEPDEKDRIGASHFRKILCCSCGKLETSFGYDFVEISIWGESFMLHQIRKMIGTAIA 304

Query: 241 VKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPIRSELFTLDESDD 300
           VKRKLLPRD+IALSL+KFSRIVLPIAPAEVLVLRGNSFSNRKRPG P R +L TL+ESDD
Sbjct: 305 VKRKLLPRDVIALSLSKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDD 364

Query: 301 IVKSVNEFYRSAILPQVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKNRE 360
           IVKSVNEFYRSAILP+VT FLDTE PPWKEWVEKLDKYTSIP+E+LDEVR+AWKAW  R 
Sbjct: 365 IVKSVNEFYRSAILPEVTNFLDTERPPWKEWVEKLDKYTSIPSEQLDEVRNAWKAWNYRV 422

[Close]

BLAST of Chy12G209430 vs. NCBI nr
Match: XP_038894774.1 (putative tRNA pseudouridine synthase isoform X1 [Benincasa hispida])

HSP 1 Score: 606 bits (1563), Expect = 1.63e-214
Identity = 302/361 (83.66%), Postives = 326/361 (90.30%), Query Frame = 0


Query: 1   MEIPESAWIDDPCGISLADYINSYLPDNINVFGIVPAQKRFDPRKECDVRKYSYLLPAKL 60
           MEIPESAW+ DPCGISLA+YIN YLPDNI VFGIVP+QK FDPRKECDVRKYSY+LPAKL
Sbjct: 148 MEIPESAWMGDPCGISLANYINFYLPDNIKVFGIVPSQKSFDPRKECDVRKYSYVLPAKL 207

Query: 61  IGIESKLETTEIDCHISDFKDILNSFEGDHPFHNYTIRSNYRTRIRAKRLVYALKSGKKV 120
           IGI +KL+TTE+D HISDFKDILNSF+G+HPFHNYTIRS YRTRIRAKR   +   G +V
Sbjct: 208 IGIGNKLKTTEVDYHISDFKDILNSFQGEHPFHNYTIRSKYRTRIRAKR---SPGYGHEV 267

Query: 121 SPGDMSNSEFEGSDQENDTFVEGVKEPDQRSPSLSVC-ENLTEVCSKLEDGSELLKDVRA 180
           SP D+SNSEFE SD E+DTFVEGVKEPDQ SPSLSVC EN TEVCSKL DGSEL KDVRA
Sbjct: 268 SPSDVSNSEFEESDGEDDTFVEGVKEPDQGSPSLSVCYENPTEVCSKLGDGSELPKDVRA 327

Query: 181 RWLHEPDEKDRIGASHFRRILYCSCGKLETYIGYDFIEISIWGESFMLHQIRKMIATAVA 240
           RWLHEPDEKDRIGASHFR+IL CSCGKLET  GYDF+EISIWGESFMLHQIRKMI TA+A
Sbjct: 328 RWLHEPDEKDRIGASHFRKILCCSCGKLETSFGYDFVEISIWGESFMLHQIRKMIGTAIA 387

Query: 241 VKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPIRSELFTLDESDD 300
           VKRKLLPRD+IALSL+KFSRIVLPIAPAEVLVLRGNSFSNRKRPG P R +L TL+ESDD
Sbjct: 388 VKRKLLPRDVIALSLSKFSRIVLPIAPAEVLVLRGNSFSNRKRPGVPKRPDLLTLNESDD 447

Query: 301 IVKSVNEFYRSAILPQVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWKNRE 360
           IVKSVNEFYRSAILP+VT FLDTE PPWKEWVEKLDKYTSIP+E+LDEVR+AWKAW  R 
Sbjct: 448 IVKSVNEFYRSAILPEVTNFLDTERPPWKEWVEKLDKYTSIPSEQLDEVRNAWKAWNYRV 505

[Close]

BLAST of Chy12G209430 vs. TAIR 10
Match: AT2G30320.1 (Pseudouridine synthase family protein )

HSP 1 Score: 353.6 bits (906), Expect = 1.9e-97
Identity = 176/356 (49.44%), Postives = 243/356 (68.26%), Query Frame = 0


Query: 1   MEIPESAWIDDPCGISLADYINSYLPDNINVFGIVPAQKRFDPRKECDVRKYSYLLPAKL 60
           MEIPE+AW DDP G  LA  I+ +LP+NI VF ++P+ +RFDPR+EC +RKYSYLLP  +
Sbjct: 139 MEIPETAWKDDPQGTVLAKCISKHLPENIRVFSVLPSNRRFDPRRECTLRKYSYLLPVDV 198

Query: 61  IGIESKLETTEIDCHISDFKDILNSFEGDHPFHNYTIRSNYRTRIRAKRLVYALKSGKKV 120
           +GI++   + EID HI+DF +IL  FEG++PFHNYT RS YR +   K     +K     
Sbjct: 199 LGIKNSFTSDEIDYHITDFNEILKEFEGEYPFHNYTQRSRYRRKSEQK-----IKQRNGR 258

Query: 121 SPGDMSNSEFEGSDQENDTFVEGVKEPDQRSPSLSVCENLTEVCSKLEDGSELLKDVRAR 180
            P +  + +   S+   +  +E  +E +++       E+   V +   D S++    RA+
Sbjct: 259 PPREPKSIKASESEFREENHIEIGEEEEEKEVD---GESDEHVVTPDSDNSQVYS--RAK 318

Query: 181 WLHEPDEKDRIGASHFRRILYCSCGKLETYIGYDFIEISIWGESFMLHQIRKMIATAVAV 240
           WL+EPDE D+I  +HFR++  C  GKLE  +G+ F+EISIWGESFMLHQIRKMI TAVAV
Sbjct: 319 WLYEPDETDKISGAHFRKVFRCRSGKLENSLGFGFVEISIWGESFMLHQIRKMIGTAVAV 378

Query: 241 KRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNRKRPGAPIRSELFTLDESDDI 300
           KR+LLPRDII LSL KF+RIVLP+AP+EVL+LRGNSF  R+ P  P    +    ES+++
Sbjct: 379 KRELLPRDIIRLSLNKFTRIVLPLAPSEVLILRGNSFEVRRLPERP---GMEATGESEEV 438

Query: 301 VKSVNEFYRSAILPQVTKFLDTESPPWKEWVEKLDKYTSIPNEELDEVRSAWKAWK 357
            K + EFYR+ ++PQV+ FLD+E  PWKEWV+ LD+   + +EEL++VR  W+ WK
Sbjct: 439 EKEIEEFYRAVMVPQVSIFLDSEKSPWKEWVDHLDRNDGLIDEELEDVRKGWEEWK 481

[Close]

BLAST of Chy12G209430 vs. TAIR 10
Match: AT1G76120.1 (Pseudouridine synthase family protein )

HSP 1 Score: 75.1 bits (183), Expect = 1.3e-13
Identity = 74/311 (23.79%), Postives = 128/311 (41.16%), Query Frame = 0


Query: 2   EIPESAWIDDPCGISLADYINSYLPDNINVFGIVPAQKRFDPRKECDVRKYSYLLPAKLI 61
           ++    +I DP G    + +NS LP+ I +FG       F  +K CD R+Y YLLP   +
Sbjct: 89  QVVSGRFIVDPLG--FVNRLNSNLPNQIRIFGYKHVTPSFSSKKFCDRRRYVYLLPVFAL 148

Query: 62  GIESKLETTEIDCHISDFKDILNSFEGDHPFHNYTIRSNYRTRIRAKRLVYALKSGKKVS 121
              S  +   +   +   ++ +  FE                  R +++   L    K +
Sbjct: 149 DPISHRDRETVMASLGPGEEYVKCFECSE---------------RGRKIPPGLVGKWKGT 208

Query: 122 PGDMSNSEFEGSDQENDTFV--EGVKEPDQRSPSLSVCENLTEVCSKLEDGSELLKD--- 181
                + +F+     N++      +K     S    +C    EV    ED  +L  +   
Sbjct: 209 NFGTKSLDFQSDISSNNSSALRSDIKIEALSSNLAGLCSVDVEVGRIQEDSCKLNTNSSE 268

Query: 182 --VRARWLHEPDEKDR--------IGASHF---------------RRILYCSCGKLETYI 241
             V++++ +   EK+R        +G+ +F               R+I+  +   +    
Sbjct: 269 TKVKSKFCYGEKEKERFSRILSCYVGSYNFHNFTTRTKADDPTANRQIISFTANTVINLD 328

Query: 242 GYDFIEISIWGESFMLHQIRKMIATAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLV 283
           G DFI+  + G+SFMLHQIRKM+  AVA+ R      +I  + +K   I +P+AP   L 
Sbjct: 329 GIDFIKCEVLGKSFMLHQIRKMMGLAVAIMRNCASESLIQSAFSKDVNITVPMAPEVGLY 382

[Close]

BLAST of Chy12G209430 vs. TAIR 10
Match: AT1G20370.1 (Pseudouridine synthase family protein )

HSP 1 Score: 74.7 bits (182), Expect = 1.7e-13
Identity = 77/307 (25.08%), Postives = 126/307 (41.04%), Query Frame = 0


Query: 8   WIDDPCGISLADYINSYLPDNINVFGIVPAQKRFDPRKECDVRKYSYLLP---------- 67
           ++D P      + +NS LPD I VFG       F  +K CD R+Y YL+P          
Sbjct: 132 YVDPP---GFVERLNSKLPDQIRVFGYKRVAPSFSSKKFCDRRRYVYLIPVFALDPCVHG 191

Query: 68  -AKLIGIESKLETTE-IDCHISDFKDILNSFEGDHPFHN-------YTIRSNYRTRIRAK 127
            A+++  +   E  + ++C    +K  L     D    +         I S+    I A 
Sbjct: 192 EAEMVRTDLGYEYVKCVECSEKGYKIPLGVMGKDTDCCDTKSLEIQSDISSSTCDAIGAD 251

Query: 128 RLVYALKSGKKVSPGDMSNSEFEGSDQENDTFVEGVKEPDQRSPSLSVCENLTEVCSKLE 187
                L S    +  ++++   +G+D         V     ++  +     L +V     
Sbjct: 252 VKCETLSSSVPSAENNLNSEVLDGAD---------VSALSSKAEGMEESNTLAKVEMNNG 311

Query: 188 DGSELLKDVRARWLHEPDEKDR--------IGASHF---------------RRILYCSCG 247
           +G ++ K   +++ +   EK+R        +G+ +F               R IL  +  
Sbjct: 312 EGEDMTKS-ESKFCYGEKEKERFNRILSYYVGSHNFHNFTTRTKAADPAANRYILSFNAN 371

Query: 248 KLETYIGYDFIEISIWGESFMLHQIRKMIATAVAVKRKLLPRDIIALSLTKFSRIVLPIA 273
            +    G DF++  + G+SFMLHQIRKMI  AVA+ R   P  +I  S  K  RI +P+A
Sbjct: 372 TVINLDGKDFVKCEVVGQSFMLHQIRKMIGLAVAIMRNYAPESLIEASFKKDVRINVPMA 425

[Close]

BLAST of Chy12G209430 vs. TAIR 10
Match: AT1G76120.2 (Pseudouridine synthase family protein )

HSP 1 Score: 67.0 bits (162), Expect = 3.5e-11
Identity = 66/285 (23.16%), Postives = 116/285 (40.70%), Query Frame = 0


Query: 2   EIPESAWIDDPCGISLADYINSYLPDNINVFGIVPAQKRFDPRKECDVRKYSYLLPAKLI 61
           ++    +I DP G    + +NS LP+ I +FG       F  +K CD R+Y YLLP   +
Sbjct: 89  QVVSGRFIVDPLG--FVNRLNSNLPNQIRIFGYKHVTPSFSSKKFCDRRRYVYLLPVFAL 148

Query: 62  GIESKLETTEIDCHISDFKDILNSFEGDHPFHNYTIRSNYRTRIRAKRLVYALKSGKKVS 121
              S  +   +   +   ++ +  FE                  R +++   L    K +
Sbjct: 149 DPISHRDRETVMASLGPGEEYVKCFECSE---------------RGRKIPPGLVGKWKGT 208

Query: 122 PGDMSNSEFEGSDQENDTFV--EGVKEPDQRSPSLSVCENLTEVCSKLEDGSELLKD--- 181
                + +F+     N++      +K     S    +C    EV    ED  +L  +   
Sbjct: 209 NFGTKSLDFQSDISSNNSSALRSDIKIEALSSNLAGLCSVDVEVGRIQEDSCKLNTNSSE 268

Query: 182 --VRARWLHEPDEKDR--------IGASHF---------------RRILYCSCGKLETYI 241
             V++++ +   EK+R        +G+ +F               R+I+  +   +    
Sbjct: 269 TKVKSKFCYGEKEKERFSRILSCYVGSYNFHNFTTRTKADDPTANRQIISFTANTVINLD 328

Query: 242 GYDFIEISIWGESFMLHQIRKMIATAVAVKRKLLPRDIIALSLTK 257
           G DFI+  + G+SFMLHQIRKM+  AVA+ R      +I  + +K
Sbjct: 329 GIDFIKCEVLGKSFMLHQIRKMMGLAVAIMRNCASESLIQSAFSK 356

The following BLAST results are available for this feature:

BLAST of Chy12G209430 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22928	2.6e-96	49.44	Putative tRNA pseudouridine synthase OS=Arabidopsis thaliana OX=3702 GN=At2g3032...	[more]
O94295	2.5e-09	25.61	tRNA pseudouridine synthase 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24...	[more]
P53167	7.1e-09	26.86	tRNA pseudouridine(27/28) synthase OS=Saccharomyces cerevisiae (strain ATCC 2045...	[more]
Q59S63	9.3e-09	28.13	tRNA pseudouridine synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876...	[more]
Q6CC39	2.2e-05	23.33	tRNA pseudouridine synthase 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) O...	[more]

BLAST of Chy12G209430 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0M0Z2	2.1e-197	95.00	PseudoU_synth_1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G53...	[more]
A0A1S3C5W1	2.4e-185	89.75	putative tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497029 PE=...	[more]
A0A6J1HM83	8.4e-162	79.44	putative tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC1114648...	[more]
A0A6J1I156	5.4e-161	79.22	putative tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111469569...	[more]
A0A6J1DMV6	2.7e-160	78.61	putative tRNA pseudouridine synthase isoform X2 OS=Momordica charantia OX=3673 G...	[more]

BLAST of Chy12G209430 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004145230.1	3.56e-249	95.00	putative tRNA pseudouridine synthase isoform X2 [Cucumis sativus] >KGN66289.2 hy...	[more]
XP_031742713.1	7.10e-249	95.00	putative tRNA pseudouridine synthase isoform X1 [Cucumis sativus]	[more]
XP_008457308.1	2.49e-233	89.75	PREDICTED: putative tRNA pseudouridine synthase [Cucumis melo]	[more]
XP_038894775.1	8.22e-216	83.66	putative tRNA pseudouridine synthase isoform X2 [Benincasa hispida]	[more]
XP_038894774.1	1.63e-214	83.66	putative tRNA pseudouridine synthase isoform X1 [Benincasa hispida]	[more]

BLAST of Chy12G209430 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G30320.1	1.9e-97	49.44	Pseudouridine synthase family protein	[more]
AT1G76120.1	1.3e-13	23.79	Pseudouridine synthase family protein	[more]
AT1G20370.1	1.7e-13	25.08	Pseudouridine synthase family protein	[more]
AT1G76120.2	3.5e-11	23.16	Pseudouridine synthase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020097	Pseudouridine synthase I, TruA, alpha/beta domain	PFAM	PF01416	PseudoU_synth_1	coord: 193..272 e-value: 1.6E-6 score: 28.5
IPR020095	Pseudouridine synthase I, TruA, C-terminal	GENE3D	3.30.70.660		coord: 48..145 e-value: 2.3E-5 score: 25.9 coord: 178..344 e-value: 1.1E-26 score: 95.5
None	No IPR available	PANTHER	PTHR11142:SF21	TRNA PSEUDOURIDINE SYNTHASE	coord: 1..355
IPR001406	Pseudouridine synthase I, TruA	PANTHER	PTHR11142	PSEUDOURIDYLATE SYNTHASE	coord: 1..355
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 193..279
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 16..112

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy12G209430.1	Chy12G209430.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0031119	tRNA pseudouridine synthesis
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding