Chy3G056260 (gene) Cucumber (hystrix) v1

BLAST of Chy3G056260 vs. ExPASy Swiss-Prot
Match: Q67ZE1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 OS=Arabidopsis thaliana OX=3702 GN=3BETAHSD/D2 PE=1 SV=2)

HSP 1 Score: 728.4 bits (1879), Expect = 6.2e-209
Identity = 358/535 (66.92%), Postives = 433/535 (80.93%), Query Frame = 0

Query: 5   EKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSGRAHY 64
           E+WCVVTGGRGFAARHLVEML++Y MF VRI DL  +I L+  E  G L E LRSGR  Y
Sbjct: 9   ERWCVVTGGRGFAARHLVEMLVRYEMFCVRIADLAPAIMLDPQEGNGVLDEGLRSGRVQY 68

Query: 65  VSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVKRL 124
           +SADLRDK+Q++KAF+G EVVFHMAAP+SSINN++L YSVNV+G +NVID+CV+  VKRL
Sbjct: 69  ISADLRDKSQVVKAFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTQNVIDACVDVGVKRL 128

Query: 125 IYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANDRNGLLTCSLRP 184
           IYTSS SVVFDG++ ++N  ESM Y  KHND YSATKAEGE L++KAN RNGLLTC +RP
Sbjct: 129 IYTSSPSVVFDGVHGILNGTESMAYPIKHNDSYSATKAEGEELIMKANGRNGLLTCCIRP 188

Query: 185 SGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAAGGVI 244
           S IFGPGDRLLVPSLVA ARAGKSKFIIGDGNN YDFTYVENV HAHVCAERALA+GG +
Sbjct: 189 SSIFGPGDRLLVPSLVAAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAERALASGGDV 248

Query: 245 SERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYKLLGP 304
           S +AAGQAYFITNMEPIKFWEF+S +LDGLGYERP IKIPA I+MPIAH+V  TYK+LGP
Sbjct: 249 STKAAGQAYFITNMEPIKFWEFMSQLLDGLGYERPSIKIPAFIMMPIAHLVELTYKVLGP 308

Query: 305 YGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQKTIEAYSHLKAEHEHK 364
           YGM VPQ TPSR+RLLSC RTF+S+KAKDRL Y P+V LQEGI++TI+++SHL A  + K
Sbjct: 309 YGMTVPQLTPSRVRLLSCSRTFDSTKAKDRLGYAPVVPLQEGIRRTIDSFSHLTAGSQSK 368

Query: 365 REGPSKASVCLRGGTAADILLWKDKRKTLIALLVLMAIYYNFIAPECTMITALSKLLMVA 424
           REGPSKAS  L GG  AD LLWKD ++TLIA+ +L++IYYNF+A   T++TALSK L+VA
Sbjct: 369 REGPSKASRILGGGKVADTLLWKDLKQTLIAIFILISIYYNFVATGSTVVTALSKALLVA 428

Query: 425 LIFLFIHGSLPEKICGYRVEKLSASCFSLSEEGSRSIAITLTSFWNSFVRNFKSLSRGND 484
            +FLF+HG LPEKI GY VEK+ AS F LS++ S  +++++ S WN+ V+  +SL +GND
Sbjct: 429 SVFLFLHGILPEKIFGYTVEKIPASQFHLSKDSSHDLSLSVISSWNTTVKALRSLCQGND 488

Query: 485 WVLLLKVAVSLLALSFIGLVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQ 540
           W    KV   LLALS  G +SL +++   +  AF  F +YEKKE+EIDS+   F+
Sbjct: 489 WSFFFKVVFVLLALSLAGAISLHSIFVIGLPIAFLAFLVYEKKEQEIDSIVVSFK 543

BLAST of Chy3G056260 vs. ExPASy Swiss-Prot
Match: Q9FX01 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 OS=Arabidopsis thaliana OX=3702 GN=3BETAHSD/D1 PE=1 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 1.0e-166
Identity = 291/445 (65.39%), Postives = 360/445 (80.90%), Query Frame = 0

Query: 5   EKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSGRAHY 64
           E+WCVVTGGRGFAARHLVEML++Y MF VRI DL  +I L   EE G LGEA+RSGR  Y
Sbjct: 9   ERWCVVTGGRGFAARHLVEMLVRYQMFHVRIADLAPAIVLNPHEETGILGEAIRSGRVQY 68

Query: 65  VSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVKRL 124
           VSADLR+K Q++K F+G EVVFHMAAP+SSINN++L YSVNV+G  NVID+C+E  VKRL
Sbjct: 69  VSADLRNKTQVVKGFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTTNVIDACIEVGVKRL 128

Query: 125 IYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANDRNGLLTCSLRP 184
           IYTSS SVVFDG++  +N DES+PY  KHND YSATKAEGEAL+LKAN R+GLLTC +RP
Sbjct: 129 IYTSSPSVVFDGVHGTLNADESLPYPPKHNDSYSATKAEGEALILKANGRSGLLTCCIRP 188

Query: 185 SGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAAGGVI 244
           S IFGPGD+L+VPSLV  ARAGKSKFIIGDG+N YDFTYVENV HAHVCAERALA+GG +
Sbjct: 189 SSIFGPGDKLMVPSLVTAARAGKSKFIIGDGSNFYDFTYVENVVHAHVCAERALASGGEV 248

Query: 245 SERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYKLLGP 304
             +AAGQAYFITNMEPIKFWEF+S +L+GLGYERP IKIPA ++MPIA++V   YKLLGP
Sbjct: 249 CAKAAGQAYFITNMEPIKFWEFMSQLLEGLGYERPSIKIPASLMMPIAYLVELAYKLLGP 308

Query: 305 YGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQKTIEAYSHLKAEHEHK 364
           YGM VP  TPSR+RLLSC RTF+SSKAKDRL Y+P+V LQEGI++TI+++SHLKA+++ K
Sbjct: 309 YGMKVPVLTPSRVRLLSCNRTFDSSKAKDRLGYSPVVPLQEGIKRTIDSFSHLKAQNQPK 368

Query: 365 REGPSKASVCLRGGTAADILLWKDKRKTLIALLVLMAIYYNFIA---PECTMITALSKLL 424
            E               + + W  K++TLIA+++L+ +Y+NF+A       +ITA+SK+L
Sbjct: 369 TE-------------VTETIQW--KKQTLIAIVILITLYHNFVATTGSSSVIITAVSKVL 428

Query: 425 MVALIFLFIHGSLPEKICGYRVEKL 447
           +V+ IF+FI+G LPEK+  +  +K+
Sbjct: 429 LVSSIFMFINGILPEKMKVFGSKKI 438

BLAST of Chy3G056260 vs. ExPASy Swiss-Prot
Match: A9X4U2 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 3 OS=Arabidopsis thaliana OX=3702 GN=3BETAHSD/D3 PE=2 SV=2)

HSP 1 Score: 395.6 bits (1015), Expect = 9.5e-109
Identity = 228/537 (42.46%), Postives = 323/537 (60.15%), Query Frame = 0

Query: 3   SEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSGRA 62
           S  K CVV GGRGF  R LV  L++   ++VR+ D G ++ L+  E    L +AL SGRA
Sbjct: 10  SHLKTCVVLGGRGFIGRSLVSRLLRLGNWTVRVADSGHTLHLD--ESDSLLEDALSSGRA 69

Query: 63  HYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVK 122
            Y   D+RDK Q++K  EG  VVF+M A +   ++Y   Y V V+G +NVI +C E  V+
Sbjct: 70  SYHCVDVRDKPQIVKVTEGSYVVFYMGATDLRSHDYFDCYKVIVQGTRNVISACRESGVR 129

Query: 123 RLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANDRNGLLTCSL 182
           +LIY S+A VVFDG   + + DES+    K     +  KA+ EAL+  AN+R+GLLTC+L
Sbjct: 130 KLIYNSTADVVFDGSQPIRDGDESLRRPLKFQSMLTDFKAQAEALIKLANNRDGLLTCAL 189

Query: 183 RPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAAGG 242
           R S +FGPGD   VP LV  A++G +KFI+G G N  DFTY ENV HAH+CA +AL +  
Sbjct: 190 RSSIVFGPGDTEFVPFLVNLAKSGYAKFILGSGENISDFTYSENVSHAHICAVKALDSQ- 249

Query: 243 VISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYKLL 302
              E  AG+ +FITN++P++FW+FVS I++GLGY RP IK+P  +V+ +  ++ WT++  
Sbjct: 250 --MEFVAGKEFFITNLKPVRFWDFVSHIVEGLGYPRPSIKLPVRLVLYVFSLLKWTHE-- 309

Query: 303 GPYGMPVPQFTPSRIRLL-SCCRTFNSSKAKDRLFYTPIVSLQEGIQKTIEAYSHLKAEH 362
              G+     T  +  LL S  RTFN + AK  L YTP+V+L++GI  T++ +S    E 
Sbjct: 310 -KEGLGSNYDTAHQYALLASSTRTFNCNAAKKHLGYTPVVTLEDGIASTLQWFSR-DLEK 369

Query: 363 EHKREGPSKASVCLRGGTAADILLWKDKRKTLIALLVLMAIYYNFIAPECTMITALSKLL 422
                  S A   L  G  ADILLW++++KT ++ LVL   YY F     T  ++ ++LL
Sbjct: 370 SDDTIIQSTADQLLGCGKVADILLWRNEKKTFVSFLVLNLFYYWFFFSGNTFTSSAAQLL 429

Query: 423 MVALIFLFIHGSLPEKICGYRVEKLSASCFSLSEEGSRSIAITLTSFWNSFVRNFKSLSR 482
            +  + L+    +P KI G++V K+    F +SE   R ++  +   WN  VR+FKSLS 
Sbjct: 430 FIFAVALYGVSFVPSKIFGFQVNKIPPWRFEISESAVRDLSSDIVVVWNQGVRSFKSLSS 489

Query: 483 GNDWVLLLKVAVSLLALSFIGLVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRF 539
           G DW+   K+A SL  L  I   SL     TV+ F+FTGFFIYE+ E E+  L + F
Sbjct: 490 GGDWIKFFKIAGSLYLLKLIVSRSLAAFLFTVMSFSFTGFFIYEQYELELYHLARIF 537

BLAST of Chy3G056260 vs. ExPASy Swiss-Prot
Match: Q9R1J0 (Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Mus musculus OX=10090 GN=Nsdhl PE=1 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 2.6e-66
Identity = 140/359 (39.00%), Postives = 204/359 (56.82%), Query Frame = 0

Query: 3   SEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSGRA 62
           S+ K C V GG GF  +H+VE L++   ++V + D+    +               + R 
Sbjct: 24  SKAKKCTVIGGSGFLGQHMVEQLLERG-YTVNVFDIHQGFD---------------NPRV 83

Query: 63  HYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVK 122
            +   DL ++  L  A +GV  VFH A+P    NN +L Y VN  G K VI++C E  V+
Sbjct: 84  QFFIGDLCNQQDLYPALKGVSTVFHCASPPPYSNNKELFYRVNFIGTKTVIETCREAGVQ 143

Query: 123 RLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKAND-RNGLLTCS 182
           +LI TSSASVVF+G++ + N  E +PYA K  D+Y+ TK   E  VL AND +   LT +
Sbjct: 144 KLILTSSASVVFEGVD-IKNGTEDLPYAMKPIDYYTETKILQERAVLDANDPKKNFLTAA 203

Query: 183 LRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAAG 242
           +RP GIFGP D  LVP L+  AR GK KF+IG+G N  DFT+VENV H H+ A   L+  
Sbjct: 204 IRPHGIFGPRDPQLVPILIDAARKGKMKFMIGNGENLVDFTFVENVVHGHILAAEHLSQD 263

Query: 243 GVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYKL 302
             +     G+A+ ITN EPI FW F+S IL GL YE P+  IP  +   +A +++    +
Sbjct: 264 AAL----GGKAFHITNDEPIPFWTFLSRILTGLNYEAPKYHIPYWMAYYLAFLLSLLVMV 323

Query: 303 LGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQKTIEAYSHLKAE 361
           + P     P FTP R+ L      ++  KAK    Y P+V++ E +++T++++ HL+ +
Sbjct: 324 VSPLIQIQPTFTPIRVALAGTFHYYSCEKAKKLFGYRPLVTMDEAVERTVQSFHHLRKD 361

BLAST of Chy3G056260 vs. ExPASy Swiss-Prot
Match: Q5PPL3 (Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Rattus norvegicus OX=10116 GN=Nsdhl PE=2 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 2.6e-66
Identity = 140/359 (39.00%), Postives = 205/359 (57.10%), Query Frame = 0

Query: 3   SEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSGRA 62
           S+ K C V GG GF  +H+VE L+    ++V + D+    +               + R 
Sbjct: 24  SKAKKCTVIGGSGFLGQHMVEQLLSRG-YAVNVFDVRQGFD---------------NPRV 83

Query: 63  HYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVK 122
            +   DL ++  L  A +GV  VFH A+P S+ NN +L Y VN  G K VI++C E  V+
Sbjct: 84  QFFIGDLCNQQDLYPALKGVSTVFHCASPPSNSNNKELFYRVNSTGTKTVIETCKEAGVQ 143

Query: 123 RLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKAND-RNGLLTCS 182
           +LI TSSASVVF+G++ + N  E +PYA K  D+Y+ TK   E  VL AND +   LT +
Sbjct: 144 KLILTSSASVVFEGVD-IKNGTEDLPYAMKPIDYYTETKILQERAVLDANDPKKNFLTAA 203

Query: 183 LRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAAG 242
           +RP GIFGP D  LVP L+  AR GK KF+IG+G N  DFT+VENV H H+ A   L+  
Sbjct: 204 IRPHGIFGPRDPQLVPVLIDAARKGKMKFMIGNGKNLVDFTFVENVVHGHILAAEHLSRD 263

Query: 243 GVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYKL 302
             +     G+A+ ITN EPI FW F+S IL GL YE P+  IP  +   +A +++    +
Sbjct: 264 AGL----GGKAFHITNDEPIPFWTFLSRILTGLNYEAPKYHIPYRVAYYLAFLLSLLVMV 323

Query: 303 LGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQKTIEAYSHLKAE 361
           L P       FTP R+ L      ++  KAK  + Y P+V++ + +++T++++ HL+ +
Sbjct: 324 LSPLIQIQTTFTPFRVALAGTFHYYSCEKAKKLIGYRPLVTMDDAVERTVQSFHHLRKD 361

BLAST of Chy3G056260 vs. ExPASy TrEMBL
Match: A0A0A0KDI8 (Reticulon-like protein OS=Cucumis sativus OX=3659 GN=Csa_6G361340 PE=4 SV=1)

HSP 1 Score: 1052.4 bits (2720), Expect = 6.9e-304
Identity = 532/541 (98.34%), Postives = 535/541 (98.89%), Query Frame = 0

Query: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
           MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELE SEEQGTLGEALRSG
Sbjct: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELETSEEQGTLGEALRSG 60

Query: 61  RAHYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
           RA YVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN
Sbjct: 61  RAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120

Query: 121 VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANDRNGLLTC 180
           VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKAN RNGLLTC
Sbjct: 121 VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANGRNGLLTC 180

Query: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
           SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA
Sbjct: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240

Query: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
           GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMV+WTYK
Sbjct: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVHWTYK 300

Query: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQKTIEAYSHLKAE 360
           LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQKTIEAYSHLKAE
Sbjct: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQKTIEAYSHLKAE 360

Query: 361 HEHKREGPSKASVCLRGGTAADILLWKDKRKTLIALLVLMAIYYNFIAPECTMITALSKL 420
           HEHKREGPSKASV LRGGTAADILLWKDKRKTLIALLVLMAIYYNFI PECTMITALSKL
Sbjct: 361 HEHKREGPSKASVYLRGGTAADILLWKDKRKTLIALLVLMAIYYNFITPECTMITALSKL 420

Query: 421 LMVALIFLFIHGSLPEKICGYRVEKLSASCFSLSEEGSRSIAITLTSFWNSFVRNFKSLS 480
           LMVALIFLFIHGSLPEKICGYRVEKLSASCFSLSEE SRSIAITLTSFWNSFVRNFKSLS
Sbjct: 421 LMVALIFLFIHGSLPEKICGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480

Query: 481 RGNDWVLLLKVAVSLLALSFIGLVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQI 540
           RGNDW+LLLKVAVSLLALSFIGLVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQ+
Sbjct: 481 RGNDWMLLLKVAVSLLALSFIGLVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV 540

Query: 541 W 542
           W
Sbjct: 541 W 541

BLAST of Chy3G056260 vs. ExPASy TrEMBL
Match: A0A1S3BDP7 (Reticulon-like protein OS=Cucumis melo OX=3656 GN=LOC103488530 PE=4 SV=1)

HSP 1 Score: 1043.5 bits (2697), Expect = 3.2e-301
Identity = 528/541 (97.60%), Postives = 534/541 (98.71%), Query Frame = 0

Query: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
           MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG
Sbjct: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60

Query: 61  RAHYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
           RA YVSADLRDKAQ+LKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN
Sbjct: 61  RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120

Query: 121 VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANDRNGLLTC 180
           VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKAN  NGLLTC
Sbjct: 121 VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANGTNGLLTC 180

Query: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
           SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA
Sbjct: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240

Query: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
           GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK
Sbjct: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300

Query: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQKTIEAYSHLKAE 360
           LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQ+TIEAYSHLKAE
Sbjct: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360

Query: 361 HEHKREGPSKASVCLRGGTAADILLWKDKRKTLIALLVLMAIYYNFIAPECTMITALSKL 420
           HE KREGPSKAS+ LRGGTAADILLWKDKRKTLIAL+VLMAIYYNFIAPECTMITALSKL
Sbjct: 361 HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL 420

Query: 421 LMVALIFLFIHGSLPEKICGYRVEKLSASCFSLSEEGSRSIAITLTSFWNSFVRNFKSLS 480
           LMVALIFLFIHGSLPEKI GYRVEKLSASCFSLSEE SRSIAITLTSFWNSFVRNFKSLS
Sbjct: 421 LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480

Query: 481 RGNDWVLLLKVAVSLLALSFIGLVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQI 540
           RGNDWVLLLKVAVSLLALSFIG+VSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQ+
Sbjct: 481 RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV 540

Query: 541 W 542
           W
Sbjct: 541 W 541

BLAST of Chy3G056260 vs. ExPASy TrEMBL
Match: A0A1S3BDV0 (Reticulon-like protein OS=Cucumis melo OX=3656 GN=LOC103488530 PE=4 SV=1)

HSP 1 Score: 985.3 bits (2546), Expect = 1.0e-283
Identity = 500/512 (97.66%), Postives = 505/512 (98.63%), Query Frame = 0

Query: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
           MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG
Sbjct: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60

Query: 61  RAHYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
           RA YVSADLRDKAQ+LKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN
Sbjct: 61  RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120

Query: 121 VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANDRNGLLTC 180
           VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKAN  NGLLTC
Sbjct: 121 VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANGTNGLLTC 180

Query: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
           SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA
Sbjct: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240

Query: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
           GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK
Sbjct: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300

Query: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQKTIEAYSHLKAE 360
           LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQ+TIEAYSHLKAE
Sbjct: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360

Query: 361 HEHKREGPSKASVCLRGGTAADILLWKDKRKTLIALLVLMAIYYNFIAPECTMITALSKL 420
           HE KREGPSKAS+ LRGGTAADILLWKDKRKTLIAL+VLMAIYYNFIAPECTMITALSKL
Sbjct: 361 HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL 420

Query: 421 LMVALIFLFIHGSLPEKICGYRVEKLSASCFSLSEEGSRSIAITLTSFWNSFVRNFKSLS 480
           LMVALIFLFIHGSLPEKI GYRVEKLSASCFSLSEE SRSIAITLTSFWNSFVRNFKSLS
Sbjct: 421 LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480

Query: 481 RGNDWVLLLKVAVSLLALSFIGLVSLQTLYKT 513
           RGNDWVLLLKVAVSLLALSFIG+VSLQTLYKT
Sbjct: 481 RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKT 512

BLAST of Chy3G056260 vs. ExPASy TrEMBL
Match: A0A6J1KG57 (Reticulon-like protein OS=Cucurbita maxima OX=3661 GN=LOC111493953 PE=4 SV=1)

HSP 1 Score: 982.2 bits (2538), Expect = 8.7e-283
Identity = 494/551 (89.66%), Postives = 520/551 (94.37%), Query Frame = 0

Query: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
           MGSEEKWCVVTGGRGFAARHLVEMLI   MFSVRIVDLGSSIELEASEEQG LGEALRSG
Sbjct: 3   MGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEQGILGEALRSG 62

Query: 61  RAHYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
           RA YVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVID+CVE+N
Sbjct: 63  RAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVEKN 122

Query: 121 VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANDRNGLLTC 180
           VKRLIYTSSASVVFDGIN L+N DE++PYASKHNDFYSATKAEGEAL+LKAN  NGLLTC
Sbjct: 123 VKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLLTC 182

Query: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
           SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIG GNNTYDFTYVENVGHAHVCAERALA+
Sbjct: 183 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERALAS 242

Query: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
           GG+I+E+AAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPA +VMPIAHMVNW Y 
Sbjct: 243 GGIIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPANLVMPIAHMVNWIYT 302

Query: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQKTIEAYSHLKAE 360
           LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGI++TIEAYSHLKA 
Sbjct: 303 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIERTIEAYSHLKAV 362

Query: 361 HEHKREGPSKASVCLRGGTAADILLWKDKRKTLIALLVLMAIYYNFIAPECTMITALSKL 420
           H+ KREGPSKAS+ LRGGTAADILLWKDKRKTLIALLVLMAIYYNFIAPECTMITALSKL
Sbjct: 363 HQQKREGPSKASIYLRGGTAADILLWKDKRKTLIALLVLMAIYYNFIAPECTMITALSKL 422

Query: 421 LMVALIFLFIHGSLPEKICGYRVEKLSASCFSLSEEGSRSIAITLTSFWNSFVRNFKSLS 480
           L+VA IFLFIHGSLPEK+ GY+VEK+S+SCFSLSEE SRSI I + SFWNSFVRNFKSLS
Sbjct: 423 LLVAFIFLFIHGSLPEKMFGYQVEKISSSCFSLSEERSRSIVINIASFWNSFVRNFKSLS 482

Query: 481 RGNDWVLLLKVAVSLLALSFIGLVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQI 540
           RGNDWV LLKV+VSLLALSFIGLVSLQTLYKTVVLFAFT FF+YEKKE+EIDSLCQ+FQ 
Sbjct: 483 RGNDWVRLLKVSVSLLALSFIGLVSLQTLYKTVVLFAFTVFFVYEKKEQEIDSLCQKFQF 542

Query: 541 WR----NVEGR 548
           W     NV G+
Sbjct: 543 WTQRKCNVPGK 553

BLAST of Chy3G056260 vs. ExPASy TrEMBL
Match: A0A6J1G542 (Reticulon-like protein OS=Cucurbita moschata OX=3662 GN=LOC111450770 PE=4 SV=1)

HSP 1 Score: 975.3 bits (2520), Expect = 1.1e-280
Identity = 488/541 (90.20%), Postives = 515/541 (95.19%), Query Frame = 0

Query: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
           MGSEEKWCVVTGGRGFAARHLVEMLI   MFSVRIVDLGSSIELEASEE+G LGEALRSG
Sbjct: 3   MGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEKGILGEALRSG 62

Query: 61  RAHYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
           RA YVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGA+NVID+CVE+N
Sbjct: 63  RAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGARNVIDACVEKN 122

Query: 121 VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANDRNGLLTC 180
           VKRLIYTSSASVVFDGIN L+N DE++PYASKHNDFYSATKAEGEAL+LKAN  NGLLTC
Sbjct: 123 VKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLLTC 182

Query: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
           SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIG GNNTYDFTYVENVGHAHVCAERALA+
Sbjct: 183 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERALAS 242

Query: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
           GG+I+E+AAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPA +VMPIAHMVNW Y 
Sbjct: 243 GGMIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPAYLVMPIAHMVNWIYT 302

Query: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQKTIEAYSHLKAE 360
           LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGI++TIEAYSHLKA 
Sbjct: 303 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIERTIEAYSHLKAV 362

Query: 361 HEHKREGPSKASVCLRGGTAADILLWKDKRKTLIALLVLMAIYYNFIAPECTMITALSKL 420
           H+ KREGPSKAS+ LRGGTAADILLWKDKRKTLIALLVLMAIYYNFIAPECTMITALSKL
Sbjct: 363 HQQKREGPSKASIYLRGGTAADILLWKDKRKTLIALLVLMAIYYNFIAPECTMITALSKL 422

Query: 421 LMVALIFLFIHGSLPEKICGYRVEKLSASCFSLSEEGSRSIAITLTSFWNSFVRNFKSLS 480
           L+VA IFLFIHG LPEK+ GY+VEK+S+SCFSLSEE SRSI I + SFWNSFVRNFKSLS
Sbjct: 423 LLVAFIFLFIHGILPEKMFGYQVEKISSSCFSLSEERSRSIVINIASFWNSFVRNFKSLS 482

Query: 481 RGNDWVLLLKVAVSLLALSFIGLVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQI 540
           RGNDWV LLKV+V+LLALSFIGLVSLQTLYKTVVLFAFT FFIYEKKE+EIDSLCQ+FQ 
Sbjct: 483 RGNDWVRLLKVSVALLALSFIGLVSLQTLYKTVVLFAFTVFFIYEKKEQEIDSLCQKFQF 542

Query: 541 W 542
           W
Sbjct: 543 W 543

BLAST of Chy3G056260 vs. NCBI nr
Match: XP_011657360.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform X1 [Cucumis sativus] >KGN47573.1 hypothetical protein Csa_018962 [Cucumis sativus])

HSP 1 Score: 1051 bits (2717), Expect = 0.0
Identity = 532/541 (98.34%), Postives = 535/541 (98.89%), Query Frame = 0

Query: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
           MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELE SEEQGTLGEALRSG
Sbjct: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELETSEEQGTLGEALRSG 60

Query: 61  RAHYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
           RA YVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN
Sbjct: 61  RAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120

Query: 121 VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANDRNGLLTC 180
           VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKAN RNGLLTC
Sbjct: 121 VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANGRNGLLTC 180

Query: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
           SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA
Sbjct: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240

Query: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
           GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMV+WTYK
Sbjct: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVHWTYK 300

Query: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQKTIEAYSHLKAE 360
           LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQKTIEAYSHLKAE
Sbjct: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQKTIEAYSHLKAE 360

Query: 361 HEHKREGPSKASVCLRGGTAADILLWKDKRKTLIALLVLMAIYYNFIAPECTMITALSKL 420
           HEHKREGPSKASV LRGGTAADILLWKDKRKTLIALLVLMAIYYNFI PECTMITALSKL
Sbjct: 361 HEHKREGPSKASVYLRGGTAADILLWKDKRKTLIALLVLMAIYYNFITPECTMITALSKL 420

Query: 421 LMVALIFLFIHGSLPEKICGYRVEKLSASCFSLSEEGSRSIAITLTSFWNSFVRNFKSLS 480
           LMVALIFLFIHGSLPEKICGYRVEKLSASCFSLSEE SRSIAITLTSFWNSFVRNFKSLS
Sbjct: 421 LMVALIFLFIHGSLPEKICGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480

Query: 481 RGNDWVLLLKVAVSLLALSFIGLVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQI 540
           RGNDW+LLLKVAVSLLALSFIGLVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQ+
Sbjct: 481 RGNDWMLLLKVAVSLLALSFIGLVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV 540

BLAST of Chy3G056260 vs. NCBI nr
Match: XP_008445550.1 (PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform X1 [Cucumis melo])

HSP 1 Score: 1042 bits (2694), Expect = 0.0
Identity = 528/541 (97.60%), Postives = 534/541 (98.71%), Query Frame = 0

Query: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
           MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG
Sbjct: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60

Query: 61  RAHYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
           RA YVSADLRDKAQ+LKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN
Sbjct: 61  RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120

Query: 121 VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANDRNGLLTC 180
           VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKAN  NGLLTC
Sbjct: 121 VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANGTNGLLTC 180

Query: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
           SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA
Sbjct: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240

Query: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
           GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK
Sbjct: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300

Query: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQKTIEAYSHLKAE 360
           LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQ+TIEAYSHLKAE
Sbjct: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360

Query: 361 HEHKREGPSKASVCLRGGTAADILLWKDKRKTLIALLVLMAIYYNFIAPECTMITALSKL 420
           HE KREGPSKAS+ LRGGTAADILLWKDKRKTLIAL+VLMAIYYNFIAPECTMITALSKL
Sbjct: 361 HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL 420

Query: 421 LMVALIFLFIHGSLPEKICGYRVEKLSASCFSLSEEGSRSIAITLTSFWNSFVRNFKSLS 480
           LMVALIFLFIHGSLPEKI GYRVEKLSASCFSLSEE SRSIAITLTSFWNSFVRNFKSLS
Sbjct: 421 LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480

Query: 481 RGNDWVLLLKVAVSLLALSFIGLVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQI 540
           RGNDWVLLLKVAVSLLALSFIG+VSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQ+
Sbjct: 481 RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQV 540

BLAST of Chy3G056260 vs. NCBI nr
Match: XP_038884192.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Benincasa hispida] >XP_038884193.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Benincasa hispida] >XP_038884195.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Benincasa hispida])

HSP 1 Score: 1003 bits (2592), Expect = 0.0
Identity = 503/539 (93.32%), Postives = 524/539 (97.22%), Query Frame = 0

Query: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
           MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQG LGEALRSG
Sbjct: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGILGEALRSG 60

Query: 61  RAHYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
           RA YVSADLRDKAQ+LKAFEG EVVFHMAAPNSSINNYKLHYSVNVEGAKNVID+CVEQN
Sbjct: 61  RAQYVSADLRDKAQVLKAFEGAEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVEQN 120

Query: 121 VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANDRNGLLTC 180
           VKRLIYTSSASVVFDGIN L N DESMPYASKHNDFYSATKAEGEALVLKAN  +GLLTC
Sbjct: 121 VKRLIYTSSASVVFDGINGLTNADESMPYASKHNDFYSATKAEGEALVLKANGSSGLLTC 180

Query: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
           SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNN YDFTYVENV HAHVCAERALA+
Sbjct: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNAYDFTYVENVAHAHVCAERALAS 240

Query: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
           GG+ISE+AAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK
Sbjct: 241 GGIISEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300

Query: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQKTIEAYSHLKAE 360
           LLGPYGMPVPQFTPSRIRLLSCCRTF+SSKAKDRLFYTPIVSLQEGIQ+TIEAYSHLKAE
Sbjct: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFDSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360

Query: 361 HEHKREGPSKASVCLRGGTAADILLWKDKRKTLIALLVLMAIYYNFIAPECTMITALSKL 420
           HEHK+EGPSKAS  LRGGTAADILLWKDKRKTL+ALLVL+AIYYNFIAPE TMITALSKL
Sbjct: 361 HEHKKEGPSKASAYLRGGTAADILLWKDKRKTLVALLVLVAIYYNFIAPESTMITALSKL 420

Query: 421 LMVALIFLFIHGSLPEKICGYRVEKLSASCFSLSEEGSRSIAITLTSFWNSFVRNFKSLS 480
           L+VALIFLFIHGSLPEKI GYRVEK+S+SCFSLSEE SRSIAIT+TSFWNSF+R+FKSLS
Sbjct: 421 LLVALIFLFIHGSLPEKIYGYRVEKISSSCFSLSEERSRSIAITVTSFWNSFIRHFKSLS 480

Query: 481 RGNDWVLLLKVAVSLLALSFIGLVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQ 539
           RGNDWVLLLKVAVSLLALSFIG++SLQTLYKTVVLFAFTGFF+YEKKE+EIDSLCQRFQ
Sbjct: 481 RGNDWVLLLKVAVSLLALSFIGVISLQTLYKTVVLFAFTGFFLYEKKEQEIDSLCQRFQ 539

BLAST of Chy3G056260 vs. NCBI nr
Match: XP_008445551.1 (PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform X2 [Cucumis melo])

HSP 1 Score: 984 bits (2545), Expect = 0.0
Identity = 500/512 (97.66%), Postives = 505/512 (98.63%), Query Frame = 0

Query: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
           MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG
Sbjct: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60

Query: 61  RAHYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
           RA YVSADLRDKAQ+LKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN
Sbjct: 61  RAQYVSADLRDKAQVLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120

Query: 121 VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANDRNGLLTC 180
           VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKAN  NGLLTC
Sbjct: 121 VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANGTNGLLTC 180

Query: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
           SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA
Sbjct: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240

Query: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
           GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK
Sbjct: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300

Query: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQKTIEAYSHLKAE 360
           LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQ+TIEAYSHLKAE
Sbjct: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQRTIEAYSHLKAE 360

Query: 361 HEHKREGPSKASVCLRGGTAADILLWKDKRKTLIALLVLMAIYYNFIAPECTMITALSKL 420
           HE KREGPSKAS+ LRGGTAADILLWKDKRKTLIAL+VLMAIYYNFIAPECTMITALSKL
Sbjct: 361 HEPKREGPSKASIYLRGGTAADILLWKDKRKTLIALIVLMAIYYNFIAPECTMITALSKL 420

Query: 421 LMVALIFLFIHGSLPEKICGYRVEKLSASCFSLSEEGSRSIAITLTSFWNSFVRNFKSLS 480
           LMVALIFLFIHGSLPEKI GYRVEKLSASCFSLSEE SRSIAITLTSFWNSFVRNFKSLS
Sbjct: 421 LMVALIFLFIHGSLPEKIYGYRVEKLSASCFSLSEERSRSIAITLTSFWNSFVRNFKSLS 480

Query: 481 RGNDWVLLLKVAVSLLALSFIGLVSLQTLYKT 512
           RGNDWVLLLKVAVSLLALSFIG+VSLQTLYKT
Sbjct: 481 RGNDWVLLLKVAVSLLALSFIGVVSLQTLYKT 512

BLAST of Chy3G056260 vs. NCBI nr
Match: XP_022999664.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita maxima] >XP_022999665.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita maxima] >XP_022999667.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita maxima] >XP_022999668.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase-like [Cucurbita maxima])

HSP 1 Score: 981 bits (2535), Expect = 0.0
Identity = 494/551 (89.66%), Postives = 520/551 (94.37%), Query Frame = 0

Query: 1   MGSEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSG 60
           MGSEEKWCVVTGGRGFAARHLVEMLI   MFSVRIVDLGSSIELEASEEQG LGEALRSG
Sbjct: 3   MGSEEKWCVVTGGRGFAARHLVEMLISSKMFSVRIVDLGSSIELEASEEQGILGEALRSG 62

Query: 61  RAHYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQN 120
           RA YVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVID+CVE+N
Sbjct: 63  RAQYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDACVEKN 122

Query: 121 VKRLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANDRNGLLTC 180
           VKRLIYTSSASVVFDGIN L+N DE++PYASKHNDFYSATKAEGEAL+LKAN  NGLLTC
Sbjct: 123 VKRLIYTSSASVVFDGINGLVNADETLPYASKHNDFYSATKAEGEALILKANGLNGLLTC 182

Query: 181 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAA 240
           SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIG GNNTYDFTYVENVGHAHVCAERALA+
Sbjct: 183 SLRPSGIFGPGDRLLVPSLVANARAGKSKFIIGKGNNTYDFTYVENVGHAHVCAERALAS 242

Query: 241 GGVISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYK 300
           GG+I+E+AAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPA +VMPIAHMVNW Y 
Sbjct: 243 GGIIAEKAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPANLVMPIAHMVNWIYT 302

Query: 301 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQKTIEAYSHLKAE 360
           LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGI++TIEAYSHLKA 
Sbjct: 303 LLGPYGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIERTIEAYSHLKAV 362

Query: 361 HEHKREGPSKASVCLRGGTAADILLWKDKRKTLIALLVLMAIYYNFIAPECTMITALSKL 420
           H+ KREGPSKAS+ LRGGTAADILLWKDKRKTLIALLVLMAIYYNFIAPECTMITALSKL
Sbjct: 363 HQQKREGPSKASIYLRGGTAADILLWKDKRKTLIALLVLMAIYYNFIAPECTMITALSKL 422

Query: 421 LMVALIFLFIHGSLPEKICGYRVEKLSASCFSLSEEGSRSIAITLTSFWNSFVRNFKSLS 480
           L+VA IFLFIHGSLPEK+ GY+VEK+S+SCFSLSEE SRSI I + SFWNSFVRNFKSLS
Sbjct: 423 LLVAFIFLFIHGSLPEKMFGYQVEKISSSCFSLSEERSRSIVINIASFWNSFVRNFKSLS 482

Query: 481 RGNDWVLLLKVAVSLLALSFIGLVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQI 540
           RGNDWV LLKV+VSLLALSFIGLVSLQTLYKTVVLFAFT FF+YEKKE+EIDSLCQ+FQ 
Sbjct: 483 RGNDWVRLLKVSVSLLALSFIGLVSLQTLYKTVVLFAFTVFFVYEKKEQEIDSLCQKFQF 542

Query: 541 WR----NVEGR 547
           W     NV G+
Sbjct: 543 WTQRKCNVPGK 553

BLAST of Chy3G056260 vs. TAIR 10
Match: AT2G26260.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 )

HSP 1 Score: 728.4 bits (1879), Expect = 4.4e-210
Identity = 358/535 (66.92%), Postives = 433/535 (80.93%), Query Frame = 0

Query: 5   EKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSGRAHY 64
           E+WCVVTGGRGFAARHLVEML++Y MF VRI DL  +I L+  E  G L E LRSGR  Y
Sbjct: 9   ERWCVVTGGRGFAARHLVEMLVRYEMFCVRIADLAPAIMLDPQEGNGVLDEGLRSGRVQY 68

Query: 65  VSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVKRL 124
           +SADLRDK+Q++KAF+G EVVFHMAAP+SSINN++L YSVNV+G +NVID+CV+  VKRL
Sbjct: 69  ISADLRDKSQVVKAFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTQNVIDACVDVGVKRL 128

Query: 125 IYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANDRNGLLTCSLRP 184
           IYTSS SVVFDG++ ++N  ESM Y  KHND YSATKAEGE L++KAN RNGLLTC +RP
Sbjct: 129 IYTSSPSVVFDGVHGILNGTESMAYPIKHNDSYSATKAEGEELIMKANGRNGLLTCCIRP 188

Query: 185 SGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAAGGVI 244
           S IFGPGDRLLVPSLVA ARAGKSKFIIGDGNN YDFTYVENV HAHVCAERALA+GG +
Sbjct: 189 SSIFGPGDRLLVPSLVAAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAERALASGGDV 248

Query: 245 SERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYKLLGP 304
           S +AAGQAYFITNMEPIKFWEF+S +LDGLGYERP IKIPA I+MPIAH+V  TYK+LGP
Sbjct: 249 STKAAGQAYFITNMEPIKFWEFMSQLLDGLGYERPSIKIPAFIMMPIAHLVELTYKVLGP 308

Query: 305 YGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQKTIEAYSHLKAEHEHK 364
           YGM VPQ TPSR+RLLSC RTF+S+KAKDRL Y P+V LQEGI++TI+++SHL A  + K
Sbjct: 309 YGMTVPQLTPSRVRLLSCSRTFDSTKAKDRLGYAPVVPLQEGIRRTIDSFSHLTAGSQSK 368

Query: 365 REGPSKASVCLRGGTAADILLWKDKRKTLIALLVLMAIYYNFIAPECTMITALSKLLMVA 424
           REGPSKAS  L GG  AD LLWKD ++TLIA+ +L++IYYNF+A   T++TALSK L+VA
Sbjct: 369 REGPSKASRILGGGKVADTLLWKDLKQTLIAIFILISIYYNFVATGSTVVTALSKALLVA 428

Query: 425 LIFLFIHGSLPEKICGYRVEKLSASCFSLSEEGSRSIAITLTSFWNSFVRNFKSLSRGND 484
            +FLF+HG LPEKI GY VEK+ AS F LS++ S  +++++ S WN+ V+  +SL +GND
Sbjct: 429 SVFLFLHGILPEKIFGYTVEKIPASQFHLSKDSSHDLSLSVISSWNTTVKALRSLCQGND 488

Query: 485 WVLLLKVAVSLLALSFIGLVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQ 540
           W    KV   LLALS  G +SL +++   +  AF  F +YEKKE+EIDS+   F+
Sbjct: 489 WSFFFKVVFVLLALSLAGAISLHSIFVIGLPIAFLAFLVYEKKEQEIDSIVVSFK 543

BLAST of Chy3G056260 vs. TAIR 10
Match: AT2G26260.2 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 )

HSP 1 Score: 609.4 bits (1570), Expect = 3.0e-174
Identity = 301/452 (66.59%), Postives = 366/452 (80.97%), Query Frame = 0

Query: 88  MAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVKRLIYTSSASVVFDGINALINTDESM 147
           MAAP+SSINN++L YSVNV+G +NVID+CV+  VKRLIYTSS SVVFDG++ ++N  ESM
Sbjct: 1   MAAPDSSINNHQLQYSVNVQGTQNVIDACVDVGVKRLIYTSSPSVVFDGVHGILNGTESM 60

Query: 148 PYASKHNDFYSATKAEGEALVLKANDRNGLLTCSLRPSGIFGPGDRLLVPSLVANARAGK 207
            Y  KHND YSATKAEGE L++KAN RNGLLTC +RPS IFGPGDRLLVPSLVA ARAGK
Sbjct: 61  AYPIKHNDSYSATKAEGEELIMKANGRNGLLTCCIRPSSIFGPGDRLLVPSLVAAARAGK 120

Query: 208 SKFIIGDGNNTYDFTYVENVGHAHVCAERALAAGGVISERAAGQAYFITNMEPIKFWEFV 267
           SKFIIGDGNN YDFTYVENV HAHVCAERALA+GG +S +AAGQAYFITNMEPIKFWEF+
Sbjct: 121 SKFIIGDGNNLYDFTYVENVAHAHVCAERALASGGDVSTKAAGQAYFITNMEPIKFWEFM 180

Query: 268 SLILDGLGYERPRIKIPACIVMPIAHMVNWTYKLLGPYGMPVPQFTPSRIRLLSCCRTFN 327
           S +LDGLGYERP IKIPA I+MPIAH+V  TYK+LGPYGM VPQ TPSR+RLLSC RTF+
Sbjct: 181 SQLLDGLGYERPSIKIPAFIMMPIAHLVELTYKVLGPYGMTVPQLTPSRVRLLSCSRTFD 240

Query: 328 SSKAKDRLFYTPIVSLQEGIQKTIEAYSHLKAEHEHKREGPSKASVCLRGGTAADILLWK 387
           S+KAKDRL Y P+V LQEGI++TI+++SHL A  + KREGPSKAS  L GG  AD LLWK
Sbjct: 241 STKAKDRLGYAPVVPLQEGIRRTIDSFSHLTAGSQSKREGPSKASRILGGGKVADTLLWK 300

Query: 388 DKRKTLIALLVLMAIYYNFIAPECTMITALSKLLMVALIFLFIHGSLPEKICGYRVEKLS 447
           D ++TLIA+ +L++IYYNF+A   T++TALSK L+VA +FLF+HG LPEKI GY VEK+ 
Sbjct: 301 DLKQTLIAIFILISIYYNFVATGSTVVTALSKALLVASVFLFLHGILPEKIFGYTVEKIP 360

Query: 448 ASCFSLSEEGSRSIAITLTSFWNSFVRNFKSLSRGNDWVLLLKVAVSLLALSFIGLVSLQ 507
           AS F LS++ S  +++++ S WN+ V+  +SL +GNDW    KV   LLALS  G +SL 
Sbjct: 361 ASQFHLSKDSSHDLSLSVISSWNTTVKALRSLCQGNDWSFFFKVVFVLLALSLAGAISLH 420

Query: 508 TLYKTVVLFAFTGFFIYEKKEREIDSLCQRFQ 540
           +++   +  AF  F +YEKKE+EIDS+   F+
Sbjct: 421 SIFVIGLPIAFLAFLVYEKKEQEIDSIVVSFK 452

BLAST of Chy3G056260 vs. TAIR 10
Match: AT1G47290.2 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 )

HSP 1 Score: 588.2 bits (1515), Expect = 7.1e-168
Identity = 291/445 (65.39%), Postives = 360/445 (80.90%), Query Frame = 0

Query: 5   EKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSGRAHY 64
           E+WCVVTGGRGFAARHLVEML++Y MF VRI DL  +I L   EE G LGEA+RSGR  Y
Sbjct: 9   ERWCVVTGGRGFAARHLVEMLVRYQMFHVRIADLAPAIVLNPHEETGILGEAIRSGRVQY 68

Query: 65  VSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVKRL 124
           VSADLR+K Q++K F+G EVVFHMAAP+SSINN++L YSVNV+G  NVID+C+E  VKRL
Sbjct: 69  VSADLRNKTQVVKGFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTTNVIDACIEVGVKRL 128

Query: 125 IYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANDRNGLLTCSLRP 184
           IYTSS SVVFDG++  +N DES+PY  KHND YSATKAEGEAL+LKAN R+GLLTC +RP
Sbjct: 129 IYTSSPSVVFDGVHGTLNADESLPYPPKHNDSYSATKAEGEALILKANGRSGLLTCCIRP 188

Query: 185 SGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAAGGVI 244
           S IFGPGD+L+VPSLV  ARAGKSKFIIGDG+N YDFTYVENV HAHVCAERALA+GG +
Sbjct: 189 SSIFGPGDKLMVPSLVTAARAGKSKFIIGDGSNFYDFTYVENVVHAHVCAERALASGGEV 248

Query: 245 SERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYKLLGP 304
             +AAGQAYFITNMEPIKFWEF+S +L+GLGYERP IKIPA ++MPIA++V   YKLLGP
Sbjct: 249 CAKAAGQAYFITNMEPIKFWEFMSQLLEGLGYERPSIKIPASLMMPIAYLVELAYKLLGP 308

Query: 305 YGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQKTIEAYSHLKAEHEHK 364
           YGM VP  TPSR+RLLSC RTF+SSKAKDRL Y+P+V LQEGI++TI+++SHLKA+++ K
Sbjct: 309 YGMKVPVLTPSRVRLLSCNRTFDSSKAKDRLGYSPVVPLQEGIKRTIDSFSHLKAQNQPK 368

Query: 365 REGPSKASVCLRGGTAADILLWKDKRKTLIALLVLMAIYYNFIA---PECTMITALSKLL 424
            E               + + W  K++TLIA+++L+ +Y+NF+A       +ITA+SK+L
Sbjct: 369 TE-------------VTETIQW--KKQTLIAIVILITLYHNFVATTGSSSVIITAVSKVL 428

Query: 425 MVALIFLFIHGSLPEKICGYRVEKL 447
           +V+ IF+FI+G LPEK+  +  +K+
Sbjct: 429 LVSSIFMFINGILPEKMKVFGSKKI 438

BLAST of Chy3G056260 vs. TAIR 10
Match: AT1G47290.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 )

HSP 1 Score: 550.4 bits (1417), Expect = 1.6e-156
Identity = 264/366 (72.13%), Postives = 311/366 (84.97%), Query Frame = 0

Query: 5   EKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSGRAHY 64
           E+WCVVTGGRGFAARHLVEML++Y MF VRI DL  +I L   EE G LGEA+RSGR  Y
Sbjct: 9   ERWCVVTGGRGFAARHLVEMLVRYQMFHVRIADLAPAIVLNPHEETGILGEAIRSGRVQY 68

Query: 65  VSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVKRL 124
           VSADLR+K Q++K F+G EVVFHMAAP+SSINN++L YSVNV+G  NVID+C+E  VKRL
Sbjct: 69  VSADLRNKTQVVKGFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTTNVIDACIEVGVKRL 128

Query: 125 IYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANDRNGLLTCSLRP 184
           IYTSS SVVFDG++  +N DES+PY  KHND YSATKAEGEAL+LKAN R+GLLTC +RP
Sbjct: 129 IYTSSPSVVFDGVHGTLNADESLPYPPKHNDSYSATKAEGEALILKANGRSGLLTCCIRP 188

Query: 185 SGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAAGGVI 244
           S IFGPGD+L+VPSLV  ARAGKSKFIIGDG+N YDFTYVENV HAHVCAERALA+GG +
Sbjct: 189 SSIFGPGDKLMVPSLVTAARAGKSKFIIGDGSNFYDFTYVENVVHAHVCAERALASGGEV 248

Query: 245 SERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYKLLGP 304
             +AAGQAYFITNMEPIKFWEF+S +L+GLGYERP IKIPA ++MPIA++V   YKLLGP
Sbjct: 249 CAKAAGQAYFITNMEPIKFWEFMSQLLEGLGYERPSIKIPASLMMPIAYLVELAYKLLGP 308

Query: 305 YGMPVPQFTPSRIRLLSCCRTFNSSKAKDRLFYTPIVSLQEGIQKTIEAYSHLKAEHEHK 364
           YGM VP  TPSR+RLLSC RTF+SSKAKDRL Y+P+V LQEGI++TI+++SHLKA+++ K
Sbjct: 309 YGMKVPVLTPSRVRLLSCNRTFDSSKAKDRLGYSPVVPLQEGIKRTIDSFSHLKAQNQPK 368

Query: 365 REGPSK 371
            E   K
Sbjct: 369 TEEKMK 374

BLAST of Chy3G056260 vs. TAIR 10
Match: AT2G43420.1 (3-beta hydroxysteroid dehydrogenase/isomerase family protein )

HSP 1 Score: 395.6 bits (1015), Expect = 6.7e-110
Identity = 228/537 (42.46%), Postives = 323/537 (60.15%), Query Frame = 0

Query: 3   SEEKWCVVTGGRGFAARHLVEMLIKYNMFSVRIVDLGSSIELEASEEQGTLGEALRSGRA 62
           S  K CVV GGRGF  R LV  L++   ++VR+ D G ++ L+  E    L +AL SGRA
Sbjct: 10  SHLKTCVVLGGRGFIGRSLVSRLLRLGNWTVRVADSGHTLHLD--ESDSLLEDALSSGRA 69

Query: 63  HYVSADLRDKAQLLKAFEGVEVVFHMAAPNSSINNYKLHYSVNVEGAKNVIDSCVEQNVK 122
            Y   D+RDK Q++K  EG  VVF+M A +   ++Y   Y V V+G +NVI +C E  V+
Sbjct: 70  SYHCVDVRDKPQIVKVTEGSYVVFYMGATDLRSHDYFDCYKVIVQGTRNVISACRESGVR 129

Query: 123 RLIYTSSASVVFDGINALINTDESMPYASKHNDFYSATKAEGEALVLKANDRNGLLTCSL 182
           +LIY S+A VVFDG   + + DES+    K     +  KA+ EAL+  AN+R+GLLTC+L
Sbjct: 130 KLIYNSTADVVFDGSQPIRDGDESLRRPLKFQSMLTDFKAQAEALIKLANNRDGLLTCAL 189

Query: 183 RPSGIFGPGDRLLVPSLVANARAGKSKFIIGDGNNTYDFTYVENVGHAHVCAERALAAGG 242
           R S +FGPGD   VP LV  A++G +KFI+G G N  DFTY ENV HAH+CA +AL +  
Sbjct: 190 RSSIVFGPGDTEFVPFLVNLAKSGYAKFILGSGENISDFTYSENVSHAHICAVKALDSQ- 249

Query: 243 VISERAAGQAYFITNMEPIKFWEFVSLILDGLGYERPRIKIPACIVMPIAHMVNWTYKLL 302
              E  AG+ +FITN++P++FW+FVS I++GLGY RP IK+P  +V+ +  ++ WT++  
Sbjct: 250 --MEFVAGKEFFITNLKPVRFWDFVSHIVEGLGYPRPSIKLPVRLVLYVFSLLKWTHE-- 309

Query: 303 GPYGMPVPQFTPSRIRLL-SCCRTFNSSKAKDRLFYTPIVSLQEGIQKTIEAYSHLKAEH 362
              G+     T  +  LL S  RTFN + AK  L YTP+V+L++GI  T++ +S    E 
Sbjct: 310 -KEGLGSNYDTAHQYALLASSTRTFNCNAAKKHLGYTPVVTLEDGIASTLQWFSR-DLEK 369

Query: 363 EHKREGPSKASVCLRGGTAADILLWKDKRKTLIALLVLMAIYYNFIAPECTMITALSKLL 422
                  S A   L  G  ADILLW++++KT ++ LVL   YY F     T  ++ ++LL
Sbjct: 370 SDDTIIQSTADQLLGCGKVADILLWRNEKKTFVSFLVLNLFYYWFFFSGNTFTSSAAQLL 429

Query: 423 MVALIFLFIHGSLPEKICGYRVEKLSASCFSLSEEGSRSIAITLTSFWNSFVRNFKSLSR 482
            +  + L+    +P KI G++V K+    F +SE   R ++  +   WN  VR+FKSLS 
Sbjct: 430 FIFAVALYGVSFVPSKIFGFQVNKIPPWRFEISESAVRDLSSDIVVVWNQGVRSFKSLSS 489

Query: 483 GNDWVLLLKVAVSLLALSFIGLVSLQTLYKTVVLFAFTGFFIYEKKEREIDSLCQRF 539
           G DW+   K+A SL  L  I   SL     TV+ F+FTGFFIYE+ E E+  L + F
Sbjct: 490 GGDWIKFFKIAGSLYLLKLIVSRSLAAFLFTVMSFSFTGFFIYEQYELELYHLARIF 537