Bhi05G000255 (gene) Wax gourd (B227) v1

Overview
NameBhi05G000255
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionGnk2-homologous domain-containing protein
Locationchr5: 7235672 .. 7236076 (-)
RNA-Seq ExpressionBhi05G000255
SyntenyBhi05G000255
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATCGGTTCTAGTGTGAAGATTTCAGCGGCTTTTTTCGCTTTCTTTGCGTTGTCTACTATGGTGGTGAGCGTTCCGAACACCGGAGTGAGGTCAGTTTTGTGCAACTCCGGAACTTTCACAGGGGGCGATCCTTTCGCAGTGAGTCTGGATTACGTTCTCAAAGAATTGGAGAGTTCAACTCCCACTATAAAGAAATATGATTTCTACAACATATCTCCTTATCCTAATTCCTTCGCTTATGGTCATGCTTCCTGCAACCAGAATCTCACAACCTCAGACTGCACCGCTTGTCTTGGGGCTGCTAAGACCAACATGTTGGGAACTTGTCAAATGAGTATTGGTGGCCGTGCGGTGCTCCATGACTGTATGATTAGGTACGAGCAATACCCATTTGATGATTAG

mRNA sequence

ATGATCGGTTCTAGTGTGAAGATTTCAGCGGCTTTTTTCGCTTTCTTTGCGTTGTCTACTATGGTGGTGAGCGTTCCGAACACCGGAGTGAGGTCAGTTTTGTGCAACTCCGGAACTTTCACAGGGGGCGATCCTTTCGCAGTGAGTCTGGATTACGTTCTCAAAGAATTGGAGAGTTCAACTCCCACTATAAAGAAATATGATTTCTACAACATATCTCCTTATCCTAATTCCTTCGCTTATGGTCATGCTTCCTGCAACCAGAATCTCACAACCTCAGACTGCACCGCTTGTCTTGGGGCTGCTAAGACCAACATGTTGGGAACTTGTCAAATGAGTATTGGTGGCCGTGCGGTGCTCCATGACTGTATGATTAGGTACGAGCAATACCCATTTGATGATTAG

Coding sequence (CDS)

ATGATCGGTTCTAGTGTGAAGATTTCAGCGGCTTTTTTCGCTTTCTTTGCGTTGTCTACTATGGTGGTGAGCGTTCCGAACACCGGAGTGAGGTCAGTTTTGTGCAACTCCGGAACTTTCACAGGGGGCGATCCTTTCGCAGTGAGTCTGGATTACGTTCTCAAAGAATTGGAGAGTTCAACTCCCACTATAAAGAAATATGATTTCTACAACATATCTCCTTATCCTAATTCCTTCGCTTATGGTCATGCTTCCTGCAACCAGAATCTCACAACCTCAGACTGCACCGCTTGTCTTGGGGCTGCTAAGACCAACATGTTGGGAACTTGTCAAATGAGTATTGGTGGCCGTGCGGTGCTCCATGACTGTATGATTAGGTACGAGCAATACCCATTTGATGATTAG

Protein sequence

MIGSSVKISAAFFAFFALSTMVVSVPNTGVRSVLCNSGTFTGGDPFAVSLDYVLKELESSTPTIKKYDFYNISPYPNSFAYGHASCNQNLTTSDCTACLGAAKTNMLGTCQMSIGGRAVLHDCMIRYEQYPFDD
Homology
BLAST of Bhi05G000255 vs. TAIR 10
Match: AT5G40380.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 42 )

HSP 1 Score: 43.1 bits (100), Expect = 2.0e-04
Identity = 33/125 (26.40%), Postives = 54/125 (43.20%), Query Frame = 0

Query: 12  FFAFFALSTMVVSVPN--TGVRSVLCNSGTFTGGDPFAVSLDYVLKELESSTPTIKKYDF 71
           F++FF L  +  S  +  T V  + C   + +  DP  +     ++++ S +  +    F
Sbjct: 16  FYSFFFLPFLSSSSDDQRTTVSGLFCGGRSKSSADPNYIPT--FVEDMHSLSLKLTTRRF 75

Query: 72  YNISPYPNSFAYGHASCNQNLTTSDCTACLGAAKTNMLGTCQMSIGGRAVLHDCMIRYEQ 131
              S    +  Y    C+ +L+ SDC  C   A+T  +  C  S   R  L  C +RYE 
Sbjct: 76  ATESLNSTTSIYALIQCHDDLSPSDCQLCYAIARTR-IPRCLPSSSARIFLDGCFLRYET 135

Query: 132 YPFDD 135
           Y F D
Sbjct: 136 YEFYD 137

BLAST of Bhi05G000255 vs. TAIR 10
Match: AT5G48540.1 (receptor-like protein kinase-related family protein )

HSP 1 Score: 42.7 bits (99), Expect = 2.6e-04
Identity = 18/51 (35.29%), Postives = 25/51 (49.02%), Query Frame = 0

Query: 82  GHASCNQNLTTSDCTACLGAAKTNMLGTCQMSIGGRAVLHDCMIRYEQYPF 133
           G   C ++L+  DC  C   A  + + TC    G R +   C +RYE YPF
Sbjct: 190 GLVQCTRDLSELDCAQCFATAVGSFMTTCHNKKGCRVLYSSCYVRYEFYPF 240

BLAST of Bhi05G000255 vs. TAIR 10
Match: AT1G70520.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 2 )

HSP 1 Score: 42.0 bits (97), Expect = 4.5e-04
Identity = 22/52 (42.31%), Postives = 26/52 (50.00%), Query Frame = 0

Query: 81  YGHASCNQNLTTSDCTACLGAAKTNMLGTCQMSIGGRAVLHDCMIRYEQYPF 133
           YG A C  +L  +DC  C   A+T ML  C    GGR  L  C +R E Y F
Sbjct: 84  YGLAQCYGDLPLNDCVLCYAEART-MLPQCYPQNGGRIFLDGCFMRAENYSF 134

BLAST of Bhi05G000255 vs. TAIR 10
Match: AT3G60720.1 (plasmodesmata-located protein 8 )

HSP 1 Score: 41.6 bits (96), Expect = 5.9e-04
Identity = 26/102 (25.49%), Postives = 42/102 (41.18%), Query Frame = 0

Query: 35  CNSGTFTGGDPFAVSLDYVLKELESSTPTIKKYDFY----NISPYPNSFAYGHASCNQNL 94
           C+   +T   PF  + D  L  + +S+       F     + S   +S  +G   C  +L
Sbjct: 34  CSPEKYTPNTPFESNRDTFLSSVVTSSSDASFNSFAVGNDSSSSSSSSAVFGLYQCRDDL 93

Query: 95  TTSDCTACLGAAKTNMLGTCQMSIGGRAVLHDCMIRYEQYPF 133
            +SDC+ C+  +   +   C  S G    L  C +RYE   F
Sbjct: 94  RSSDCSKCIQTSVDQITLICPYSYGASLQLEGCFLRYETNDF 135

BLAST of Bhi05G000255 vs. ExPASy Swiss-Prot
Match: D9IWE0 (Antifungal protein ginkbilobin-like protein OS=Pinus pinaster OX=71647 GN=EAP PE=2 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 1.4e-13
Identity = 50/125 (40.00%), Postives = 63/125 (50.40%), Query Frame = 0

Query: 16  FALSTMVVSV--------PNTGVRSVLCNSGTFTGGDPFAVSLDYVLKELESSTPTIKKY 75
           F L+ MVV++        P T + S  CN      G  F  +L  +L +LE +T +   Y
Sbjct: 7   FTLAVMVVALVVGSAVGTPRTNLVSSACNGQKIQSGSSFYSTLGSLLVDLEGNTAS-SGY 66

Query: 76  DFYNISPYPNSFAYGHASCNQNLTTSDCTACLGAAKTNMLGTCQMSIGGRAVLHDCMIRY 133
           D Y  S   +  AYG A CNQ L+ SDCTACL      +   C  +IG R  L DC IRY
Sbjct: 67  D-YKASRAGSPTAYGRAVCNQGLSQSDCTACLKNLGGRIWNICGYAIGARVQLTDCFIRY 126

BLAST of Bhi05G000255 vs. ExPASy Swiss-Prot
Match: A9NQT1 (Antifungal protein ginkbilobin-like protein 1 OS=Picea sitchensis OX=3332 PE=2 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 6.7e-13
Identity = 50/126 (39.68%), Postives = 65/126 (51.59%), Query Frame = 0

Query: 16  FALSTMVVSV--------PNTGVRSVLCNSGTFTGGDPFAVSLDYVLKELESSTPTIKKY 75
           FAL+ MV++V        PNT   S  CN+     G+PF  +L  +L  LE +T     Y
Sbjct: 8   FALAVMVLAVLVASAAGAPNTNFVSSACNTKKIPSGNPFFNNLGALLVNLEKNT-AFSGY 67

Query: 76  DFYNISPYPNS---FAYGHASCNQNLTTSDCTACLGAAKTNMLGTCQMSIGGRAVLHDCM 131
           D Y  S   +     AYG   C Q+++ SDCTACL      + G C+ +IG R  L DC 
Sbjct: 68  D-YKASRAGSGGAPTAYGRGVCKQSISQSDCTACLTNLGGRIWGICKNAIGARVQLTDCF 127

BLAST of Bhi05G000255 vs. ExPASy Swiss-Prot
Match: Q40858 (Antifungal protein ginkbilobin-like protein 2 OS=Picea glauca OX=3330 GN=EMB35 PE=2 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 7.5e-12
Identity = 48/126 (38.10%), Postives = 65/126 (51.59%), Query Frame = 0

Query: 16  FALSTMVVSV--------PNTGVRSVLCNSGTFTGGDPFAVSLDYVLKELESSTPTIKKY 75
           FAL+ MV++V        PNT + S  CN      G+PF  +L  +L +LE +T     +
Sbjct: 8   FALAVMVLAVLVASAAGAPNTNLVSSACNGNKIPSGNPFFNNLGALLVDLEKNT-AFSGF 67

Query: 76  DFYNISPYPNS---FAYGHASCNQNLTTSDCTACLGAAKTNMLGTCQMSIGGRAVLHDCM 131
           D Y  S   +     AYG   C Q+++ SDCTACL      + G  + +IG R  L DC 
Sbjct: 68  D-YKASRAGSGGAPTAYGRGVCKQSISQSDCTACLTNLGGRIWGISKNAIGARVQLTDCF 127

BLAST of Bhi05G000255 vs. ExPASy Swiss-Prot
Match: A4ZDL6 (Antifungal protein ginkbilobin-2 OS=Ginkgo biloba OX=3311 GN=GNK2 PE=1 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 1.7e-11
Identity = 44/133 (33.08%), Postives = 66/133 (49.62%), Query Frame = 0

Query: 5   SVKISAAFFAFFALST---MVVSVPNTGVRSVLCNSGTFTGGDPFAVSLDYVLKELESST 64
           ++++++AF   FAL+    ++    NT   S  CN+     G PF  +L  +L +L  +T
Sbjct: 3   TMRMNSAFILAFALAAAMLILTEAANTAFVSSACNTQKIPSGSPFNRNLRAMLADLRQNT 62

Query: 65  PTIKKYDFYNISPYPNS--FAYGHASCNQNLTTSDCTACLGAAKTNMLGTCQMSIGGRAV 124
                YD+            AYG A+C Q+++ SDCTACL      +   C  +IG R  
Sbjct: 63  -AFSGYDYKTSRAGSGGAPTAYGRATCKQSISQSDCTACLSNLVNRIFSICNNAIGARVQ 122

Query: 125 LHDCMIRYEQYPF 133
           L DC I+YEQ  F
Sbjct: 123 LVDCFIQYEQRSF 134

BLAST of Bhi05G000255 vs. ExPASy Swiss-Prot
Match: D5A8I3 (Antifungal protein ginkbilobin-like protein 2 OS=Picea sitchensis OX=3332 PE=2 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 5.3e-10
Identity = 37/107 (34.58%), Postives = 54/107 (50.47%), Query Frame = 0

Query: 27  NTGVRSVLCNSGTFTGGDPFAVSLDYVLKELESSTPTI-KKYDFYNISPYPNSFAYGHAS 86
           NT   S  CN+     G PF  +L   +++L  +T  I   Y   ++       AYG A 
Sbjct: 25  NTKFVSASCNTEKIARGSPFFNNLRDAIEDLRQNTAYIGYDYKTSHVGSGGAPTAYGRAI 84

Query: 87  CNQNLTTSDCTACLGAAKTNMLGTCQMSIGGRAVLHDCMIRYEQYPF 133
           C Q+++ S+CT+CL    T + G C  +IG R  L DC ++YEQ  F
Sbjct: 85  CKQSISQSNCTSCLSNVATRIYGICGNAIGARVQLTDCYMQYEQRSF 131

BLAST of Bhi05G000255 vs. ExPASy TrEMBL
Match: A0A0A0KGA2 (Gnk2-homologous domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G409950 PE=4 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 3.4e-60
Identity = 114/134 (85.07%), Postives = 124/134 (92.54%), Query Frame = 0

Query: 1   MIGSSVKISAAFFAFFALSTMVVSVPNTGVRSVLCNSGTFTGGDPFAVSLDYVLKELESS 60
           MIGSS+KIS AF AFFALS MVVS+PNTGVRSVLCNSGTFTGGDPF VSLDYVLKEL+S+
Sbjct: 1   MIGSSLKISVAFLAFFALSIMVVSIPNTGVRSVLCNSGTFTGGDPFTVSLDYVLKELQSA 60

Query: 61  TPTIKKYDFYNISPYPNSFAYGHASCNQNLTTSDCTACLGAAKTNMLGTCQMSIGGRAVL 120
           TPT+K YDFYNISPYPN+FAYGHASCNQNL+TSDCT CL AAK NMLG+CQM IGGR+VL
Sbjct: 61  TPTVKNYDFYNISPYPNAFAYGHASCNQNLSTSDCTTCLEAAKNNMLGSCQMRIGGRSVL 120

Query: 121 HDCMIRYEQYPFDD 135
           +DC IRYEQYPFDD
Sbjct: 121 NDCTIRYEQYPFDD 134

BLAST of Bhi05G000255 vs. ExPASy TrEMBL
Match: A0A5D3CNH9 (Antifungal protein ginkbilobin-2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold496G00580 PE=4 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 7.6e-60
Identity = 114/134 (85.07%), Postives = 125/134 (93.28%), Query Frame = 0

Query: 1   MIGSSVKISAAFFAFFALSTMVVSVPNTGVRSVLCNSGTFTGGDPFAVSLDYVLKELESS 60
           MIGSS+KIS AF AFFA+S MVVS+PNTGVRSVLCNSGTFTGGDPFAVSLDYVLKEL+S+
Sbjct: 1   MIGSSLKISVAFLAFFAVSIMVVSIPNTGVRSVLCNSGTFTGGDPFAVSLDYVLKELQSA 60

Query: 61  TPTIKKYDFYNISPYPNSFAYGHASCNQNLTTSDCTACLGAAKTNMLGTCQMSIGGRAVL 120
           TPTIK YDFYNISPYPN+FAYGHASCNQNLTTSDCT+CL AA+ NMLG+CQ  IGGR+VL
Sbjct: 61  TPTIKNYDFYNISPYPNAFAYGHASCNQNLTTSDCTSCLEAAENNMLGSCQKRIGGRSVL 120

Query: 121 HDCMIRYEQYPFDD 135
           +DC IRYEQYPFDD
Sbjct: 121 NDCTIRYEQYPFDD 134

BLAST of Bhi05G000255 vs. ExPASy TrEMBL
Match: A0A6J1ESI5 (antifungal protein ginkbilobin-2-like OS=Cucurbita moschata OX=3662 GN=LOC111437337 PE=4 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 5.9e-52
Identity = 101/114 (88.60%), Postives = 105/114 (92.11%), Query Frame = 0

Query: 21  MVVSVPNTGVRSVLCNSGTFTGGDPFAVSLDYVLKELESSTPTIKKYDFYNISPYPNSFA 80
           MVVSVPNTGV+SVLCNSG FTGGDPFAVSLDYVLKELESSTPT   YDFYNISPYPNSFA
Sbjct: 1   MVVSVPNTGVKSVLCNSGIFTGGDPFAVSLDYVLKELESSTPTTNNYDFYNISPYPNSFA 60

Query: 81  YGHASCNQNLTTSDCTACLGAAKTNMLGTCQMSIGGRAVLHDCMIRYEQYPFDD 135
           YGHASCNQ+LTTSDCT CLGAAKTNMLG+CQM IG RAVL+DC IRYEQ PFDD
Sbjct: 61  YGHASCNQSLTTSDCTTCLGAAKTNMLGSCQMRIGARAVLNDCSIRYEQNPFDD 114

BLAST of Bhi05G000255 vs. ExPASy TrEMBL
Match: A0A6J5YEQ2 (Gnk2-homologous domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS52084 PE=4 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 1.2e-44
Identity = 90/127 (70.87%), Postives = 104/127 (81.89%), Query Frame = 0

Query: 8   ISAAFFAFFALSTMVVSVPNTGVRSVLCNSGTFTGGDPFAVSLDYVLKELESSTPTIKKY 67
           I   F  FF+++T   S P+T + SVLCNSG +T GDPFAVSL YVL+ELE++T T K Y
Sbjct: 13  IVVVFLGFFSVAT---STPDTNITSVLCNSGVYTAGDPFAVSLAYVLQELETATSTHKNY 72

Query: 68  DFYNISPYPNSFAYGHASCNQNLTTSDCTACLGAAKTNMLGTCQMSIGGRAVLHDCMIRY 127
           DFY+ISPYPN+FAYGHASCNQN+TT DCT CLGAAKT+MLGTCQ +IGGRAVL DC IRY
Sbjct: 73  DFYDISPYPNAFAYGHASCNQNITTPDCTTCLGAAKTDMLGTCQNAIGGRAVLRDCAIRY 132

Query: 128 EQYPFDD 135
           EQYPF D
Sbjct: 133 EQYPFTD 136

BLAST of Bhi05G000255 vs. ExPASy TrEMBL
Match: A0A5E4EE49 (PREDICTED: antifungal OS=Prunus dulcis OX=3755 GN=ALMOND_2B017312 PE=4 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 1.5e-44
Identity = 91/130 (70.00%), Postives = 104/130 (80.00%), Query Frame = 0

Query: 6   VKISAAFFAFF-ALSTMVVSVPNTGVRSVLCNSGTFTGGDPFAVSLDYVLKELESSTPTI 65
           +KI+A    FF     +  S P+T + SVLCNSG +T GDPFAVSL YVLKELE++T T 
Sbjct: 6   MKIAATLVVFFIGFFGVATSTPDTNITSVLCNSGLYTAGDPFAVSLAYVLKELETATSTH 65

Query: 66  KKYDFYNISPYPNSFAYGHASCNQNLTTSDCTACLGAAKTNMLGTCQMSIGGRAVLHDCM 125
           K YDFY+ISPYPN+FAYGHASCNQN+TT DCT CLGAAKT+MLGTCQ +IGGRAVL DC 
Sbjct: 66  KNYDFYDISPYPNAFAYGHASCNQNITTPDCTTCLGAAKTDMLGTCQNAIGGRAVLRDCA 125

Query: 126 IRYEQYPFDD 135
           IRYEQYPF D
Sbjct: 126 IRYEQYPFTD 135

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G40380.12.0e-0426.40cysteine-rich RLK (RECEPTOR-like protein kinase) 42 [more]
AT5G48540.12.6e-0435.29receptor-like protein kinase-related family protein [more]
AT1G70520.14.5e-0442.31cysteine-rich RLK (RECEPTOR-like protein kinase) 2 [more]
AT3G60720.15.9e-0425.49plasmodesmata-located protein 8 [more]
Match NameE-valueIdentityDescription
D9IWE01.4e-1340.00Antifungal protein ginkbilobin-like protein OS=Pinus pinaster OX=71647 GN=EAP PE... [more]
A9NQT16.7e-1339.68Antifungal protein ginkbilobin-like protein 1 OS=Picea sitchensis OX=3332 PE=2 S... [more]
Q408587.5e-1238.10Antifungal protein ginkbilobin-like protein 2 OS=Picea glauca OX=3330 GN=EMB35 P... [more]
A4ZDL61.7e-1133.08Antifungal protein ginkbilobin-2 OS=Ginkgo biloba OX=3311 GN=GNK2 PE=1 SV=1[more]
D5A8I35.3e-1034.58Antifungal protein ginkbilobin-like protein 2 OS=Picea sitchensis OX=3332 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A0A0KGA23.4e-6085.07Gnk2-homologous domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G40... [more]
A0A5D3CNH97.6e-6085.07Antifungal protein ginkbilobin-2-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A6J1ESI55.9e-5288.60antifungal protein ginkbilobin-2-like OS=Cucurbita moschata OX=3662 GN=LOC111437... [more]
A0A6J5YEQ21.2e-4470.87Gnk2-homologous domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP... [more]
A0A5E4EE491.5e-4470.00PREDICTED: antifungal OS=Prunus dulcis OX=3755 GN=ALMOND_2B017312 PE=4 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002902Gnk2-homologous domainPFAMPF01657Stress-antifungcoord: 35..130
e-value: 5.7E-14
score: 52.5
IPR002902Gnk2-homologous domainPROSITEPS51473GNK2coord: 28..132
score: 16.244444
IPR038408Gnk2-homologous domain superfamilyGENE3D3.30.430.20coord: 27..132
e-value: 6.7E-18
score: 66.7
NoneNo IPR availablePANTHERPTHR32080:SF54ANTIFUNGAL PROTEIN GINKBILOBIN-2-LIKEcoord: 6..133
NoneNo IPR availablePANTHERPTHR32080ANTIFUNGAL PROTEIN GINKBILOBIN-2-LIKEcoord: 6..133

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi05M000255Bhi05M000255mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0009506 plasmodesma