Sgr029579 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr029579
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionDelta(24)-sterol reductase
Locationtig00153403: 2103169 .. 2105065 (+)
RNA-Seq ExpressionSgr029579
SyntenySgr029579
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCAGATCTAGCAACTCCTCTGCGCCCGAAGAGGAAGAAGGTCTGGGTGGACTACTTTGTTCAATTCAGATGGATTTTAGTTGTTTTTGTTGTCCTTCCTGTATCATTCAGCCTATACTTCTTCACATATCTTGGAGACGTCAGATCTGCGTGGAAGTCCTATAAGCAGCGCCAGAAAGAACATGAAGAAAATGTCAAGAAAATTGCAAAGCGCCTGAAAGAGAGGAACCCAGCAAAAGATGGTCTTGTTTGCACAGCCCGCAAACCCTGGATTGCTGTTGGAATGCGAAATGTTGACTACAAACGAGCACGCCACTTTGGGGTTGATTTATCAGCTTTCCGGAATATTTTGGAAATTGACAAAGACAGAATGATTGCTAAAGTTGAGCCCCTCGTGAACATGGGGCAAATCAGTAGGGCCACTTTGCCACTGAATCTTTCTCTTGCTGTAGTTGCTGAACTTGATGATCTTACTGTCGGGGGACTTATCAATGGCTATGGAATAGAAGGAAGCTCCCATATCTATGGCTTGTTTTCCGACACTGTTGTGGCATATGAGATTGTCCTAGCAGACGGCCGGGTTGTTAGAGCTACCAAAGATAATGAGTACTCGGATCTTTTCTATGCAATCCCATGGTCCCAAGGAACAATTGGTCTTCTTGTCGCTGCTGAGATCAAGCTTATACCCATTAAAGAGTATATGAAAGTTACATATACACCTTTCATAGGCACTTTAAAAGAGATAGGACAAGCTTACATAGATTCTCTTGCACCTAGAGATGGAGATCAAGATAATCCAGAAAAGGTTCCCGACTTTGTAGAAACTATGATTTACACCCCTTCTGAAGCCGTCGTCATGACAGGTAGATATGCCTCCAAAGAAGAGGCCAAGAAAAGGGGAAATGTGATCAACAGTATTGGATGGTGGTTCAAACCCTGGTTCTACCAGCATGCAGCTACGGCACTCAAGAGAGGGCCGTTTGTGGAGTACATCCCTACCAGGGAGTACTATCATAGGCACACCCGATGTTTGTATTGGGAGGGAAAACTTATCCTTCCATTTGCAGATCAATGGTGGTTCAGATTTTTATTGGGATGGATGATGCCTCCCAAAGTTTCTCTGCTCAAAGCTACTCAAGGCGAAGCTATCAGAAATTATTATCATGAAATGCACATAATTCAAGACATGCTTGTTCCTCTTTACAAGGTTCCAGATGCCTTGGAGTGGGCCCATCGTGAGTTGGAGGTATTGCTTTTCACCTCAATACTTTCTTTTACTTTATAACTAGCTGGACAACTTCCATTCCATCCTGAACTTGTATATTTGCTGGAGGTGGTTATAGGGAAATGGTTGACGAGTCTATCAGAACAGAAGTGGATTTCAATTGAAGTATAGGCACTTCATTTGATTAACAACAGCAATTTGTTTGTGTTCTGCGTAGGTTTACCCCATTTGGCTCTGCCCACACAGACTCTTCAAACTTCCAGTCAAAACCATGATATATCCCGAACCAGGATTCGAGTTGCATCGTCGACAGGGAGACACACCTTATGCTCAGATGTATACAGATATTGGAATCTACTATGCCCCAGGGCCTGTCCTGAGGGGTGAAGTTTTCGACGGTGCAGGGGCTGTCCGCAGAATGGAGAAATGGCTTCTGGAAAATCATGGTTTTCAGGCTCTATATGCAGTGTCAGAACTGAATGAGAAAGATTTCTGGAGAATGTTTGACGCTGAACTCTATAACCAATGCCGGAAGAAGTATGGTGCTGTAGGAACTTTCATGACCGTGTACTACAAGAGCAAGAAAGGGCGGAAAACTGAGAAGGAGGTGAAGGAAGCTGAACAAGCTCACCTTGAAACTACTTATGCTGAACTGGATCAACCCCAATAA

mRNA sequence

ATGTCAGATCTAGCAACTCCTCTGCGCCCGAAGAGGAAGAAGGTCTGGGTGGACTACTTTGTTCAATTCAGATGGATTTTAGTTGTTTTTGTTGTCCTTCCTGTATCATTCAGCCTATACTTCTTCACATATCTTGGAGACGTCAGATCTGCGTGGAAGTCCTATAAGCAGCGCCAGAAAGAACATGAAGAAAATGTCAAGAAAATTGCAAAGCGCCTGAAAGAGAGGAACCCAGCAAAAGATGGTCTTGTTTGCACAGCCCGCAAACCCTGGATTGCTGTTGGAATGCGAAATGTTGACTACAAACGAGCACGCCACTTTGGGGTTGATTTATCAGCTTTCCGGAATATTTTGGAAATTGACAAAGACAGAATGATTGCTAAAGTTGAGCCCCTCGTGAACATGGGGCAAATCAGTAGGGCCACTTTGCCACTGAATCTTTCTCTTGCTGTAGTTGCTGAACTTGATGATCTTACTGTCGGGGGACTTATCAATGGCTATGGAATAGAAGGAAGCTCCCATATCTATGGCTTGTTTTCCGACACTGTTGTGGCATATGAGATTGTCCTAGCAGACGGCCGGGTTGTTAGAGCTACCAAAGATAATGAGTACTCGGATCTTTTCTATGCAATCCCATGGTCCCAAGGAACAATTGGTCTTCTTGTCGCTGCTGAGATCAAGCTTATACCCATTAAAGAGTATATGAAAGTTACATATACACCTTTCATAGGCACTTTAAAAGAGATAGGACAAGCTTACATAGATTCTCTTGCACCTAGAGATGGAGATCAAGATAATCCAGAAAAGGTTCCCGACTTTGTAGAAACTATGATTTACACCCCTTCTGAAGCCGTCGTCATGACAGGTAGATATGCCTCCAAAGAAGAGGCCAAGAAAAGGGGAAATGTGATCAACAGTATTGGATGGTGGTTCAAACCCTGGTTCTACCAGCATGCAGCTACGGCACTCAAGAGAGGGCCGTTTGTGGAGTACATCCCTACCAGGGAGTACTATCATAGGCACACCCGATGTTTGTATTGGGAGGGAAAACTTATCCTTCCATTTGCAGATCAATGGTGGTTCAGATTTTTATTGGGATGGATGATGCCTCCCAAAGTTTCTCTGCTCAAAGCTACTCAAGGCGAAGCTATCAGAAATTATTATCATGAAATGCACATAATTCAAGACATGCTTGTTCCTCTTTACAAGGTTCCAGATGCCTTGGAGTGGGCCCATCGTGAGTTGGAGGTTTACCCCATTTGGCTCTGCCCACACAGACTCTTCAAACTTCCAGTCAAAACCATGATATATCCCGAACCAGGATTCGAGTTGCATCGTCGACAGGGAGACACACCTTATGCTCAGATGTATACAGATATTGGAATCTACTATGCCCCAGGGCCTGTCCTGAGGGGTGAAGTTTTCGACGGTGCAGGGGCTGTCCGCAGAATGGAGAAATGGCTTCTGGAAAATCATGGTTTTCAGGCTCTATATGCAGTGTCAGAACTGAATGAGAAAGATTTCTGGAGAATGTTTGACGCTGAACTCTATAACCAATGCCGGAAGAAGTATGGTGCTGTAGGAACTTTCATGACCGTGTACTACAAGAGCAAGAAAGGGCGGAAAACTGAGAAGGAGGTGAAGGAAGCTGAACAAGCTCACCTTGAAACTACTTATGCTGAACTGGATCAACCCCAATAA

Coding sequence (CDS)

ATGTCAGATCTAGCAACTCCTCTGCGCCCGAAGAGGAAGAAGGTCTGGGTGGACTACTTTGTTCAATTCAGATGGATTTTAGTTGTTTTTGTTGTCCTTCCTGTATCATTCAGCCTATACTTCTTCACATATCTTGGAGACGTCAGATCTGCGTGGAAGTCCTATAAGCAGCGCCAGAAAGAACATGAAGAAAATGTCAAGAAAATTGCAAAGCGCCTGAAAGAGAGGAACCCAGCAAAAGATGGTCTTGTTTGCACAGCCCGCAAACCCTGGATTGCTGTTGGAATGCGAAATGTTGACTACAAACGAGCACGCCACTTTGGGGTTGATTTATCAGCTTTCCGGAATATTTTGGAAATTGACAAAGACAGAATGATTGCTAAAGTTGAGCCCCTCGTGAACATGGGGCAAATCAGTAGGGCCACTTTGCCACTGAATCTTTCTCTTGCTGTAGTTGCTGAACTTGATGATCTTACTGTCGGGGGACTTATCAATGGCTATGGAATAGAAGGAAGCTCCCATATCTATGGCTTGTTTTCCGACACTGTTGTGGCATATGAGATTGTCCTAGCAGACGGCCGGGTTGTTAGAGCTACCAAAGATAATGAGTACTCGGATCTTTTCTATGCAATCCCATGGTCCCAAGGAACAATTGGTCTTCTTGTCGCTGCTGAGATCAAGCTTATACCCATTAAAGAGTATATGAAAGTTACATATACACCTTTCATAGGCACTTTAAAAGAGATAGGACAAGCTTACATAGATTCTCTTGCACCTAGAGATGGAGATCAAGATAATCCAGAAAAGGTTCCCGACTTTGTAGAAACTATGATTTACACCCCTTCTGAAGCCGTCGTCATGACAGGTAGATATGCCTCCAAAGAAGAGGCCAAGAAAAGGGGAAATGTGATCAACAGTATTGGATGGTGGTTCAAACCCTGGTTCTACCAGCATGCAGCTACGGCACTCAAGAGAGGGCCGTTTGTGGAGTACATCCCTACCAGGGAGTACTATCATAGGCACACCCGATGTTTGTATTGGGAGGGAAAACTTATCCTTCCATTTGCAGATCAATGGTGGTTCAGATTTTTATTGGGATGGATGATGCCTCCCAAAGTTTCTCTGCTCAAAGCTACTCAAGGCGAAGCTATCAGAAATTATTATCATGAAATGCACATAATTCAAGACATGCTTGTTCCTCTTTACAAGGTTCCAGATGCCTTGGAGTGGGCCCATCGTGAGTTGGAGGTTTACCCCATTTGGCTCTGCCCACACAGACTCTTCAAACTTCCAGTCAAAACCATGATATATCCCGAACCAGGATTCGAGTTGCATCGTCGACAGGGAGACACACCTTATGCTCAGATGTATACAGATATTGGAATCTACTATGCCCCAGGGCCTGTCCTGAGGGGTGAAGTTTTCGACGGTGCAGGGGCTGTCCGCAGAATGGAGAAATGGCTTCTGGAAAATCATGGTTTTCAGGCTCTATATGCAGTGTCAGAACTGAATGAGAAAGATTTCTGGAGAATGTTTGACGCTGAACTCTATAACCAATGCCGGAAGAAGTATGGTGCTGTAGGAACTTTCATGACCGTGTACTACAAGAGCAAGAAAGGGCGGAAAACTGAGAAGGAGGTGAAGGAAGCTGAACAAGCTCACCTTGAAACTACTTATGCTGAACTGGATCAACCCCAATAA

Protein sequence

MSDLATPLRPKRKKVWVDYFVQFRWILVVFVVLPVSFSLYFFTYLGDVRSAWKSYKQRQKEHEENVKKIAKRLKERNPAKDGLVCTARKPWIAVGMRNVDYKRARHFGVDLSAFRNILEIDKDRMIAKVEPLVNMGQISRATLPLNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFSDTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYTPFIGTLKEIGQAYIDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKRGNVINSIGWWFKPWFYQHAATALKRGPFVEYIPTREYYHRHTRCLYWEGKLILPFADQWWFRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIIQDMLVPLYKVPDALEWAHRELEVYPIWLCPHRLFKLPVKTMIYPEPGFELHRRQGDTPYAQMYTDIGIYYAPGPVLRGEVFDGAGAVRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYNQCRKKYGAVGTFMTVYYKSKKGRKTEKEVKEAEQAHLETTYAELDQPQ
Homology
BLAST of Sgr029579 vs. NCBI nr
Match: KAG7014278.1 (Delta(24)-sterol reductase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1110.9 bits (2872), Expect = 0.0e+00
Identity = 527/565 (93.27%), Postives = 552/565 (97.70%), Query Frame = 0

Query: 1   MSDLATPLRPKRKKVWVDYFVQFRWILVVFVVLPVSFSLYFFTYLGDVRSAWKSYKQRQK 60
           MSDL  PLRPKRKKVWVDYFVQFRWILV+FVVLP+SFSLYFFTYLGDV+SAWKSY++RQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVKSAWKSYEKRQK 60

Query: 61  EHEENVKKIAKRLKERNPAKDGLVCTARKPWIAVGMRNVDYKRARHFGVDLSAFRNILEI 120
           EH+ENVKK+ KRLK+RNP KDGLVCTARKPWIAVGMRNVDYKRARHF VDLSAFRNIL+I
Sbjct: 61  EHDENVKKVVKRLKQRNPTKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKDRMIAKVEPLVNMGQISRATLPLNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DK+RMIAKVEPLVNMGQISRAT+PLNLSLAVVAELDDLTVGGL+NGYGIEGSSH+YGLFS
Sbjct: 121 DKERMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLVNGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYT 240
           DTVVAYEIVLADG+VVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTY 
Sbjct: 181 DTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PFIGTLKEIGQAYIDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKR 300
           PFIGTL+EIGQAY+DSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVM+GRYASKEEAKK+
Sbjct: 241 PFIGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMSGRYASKEEAKKK 300

Query: 301 GNVINSIGWWFKPWFYQHAATALKRGPFVEYIPTREYYHRHTRCLYWEGKLILPFADQWW 360
           GNVIN IGWWFKPWFYQHAATALK+G FVEYIPTR+YYHRHTR LYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHAATALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIIQDMLVPLYKVPDALEWAHRELEVYPI 420
           FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMH+IQDMLVPLYKVPDALEWAHRE EVYPI
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPI 420

Query: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTPYAQMYTDIGIYYAPGPVLRGEVFDGAGA 480
           WLCPHRLFKLPVKTMIYPEPGFELHRRQGDT YAQMYTD+G+YYAPGPVLRGEVFDGA A
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYNQCRKKYGAVGTFMTVYYKSKKG 540
           VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELY QCRKKYGAVGTFMTVYYK+KKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKTKKG 540

Query: 541 RKTEKEVKEAEQAHLETTYAELDQP 566
           RKTEKEVKEAEQAHLETTYAEL+QP
Sbjct: 541 RKTEKEVKEAEQAHLETTYAELEQP 565

BLAST of Sgr029579 vs. NCBI nr
Match: XP_023548025.1 (delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548027.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548028.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548029.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548030.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548031.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548032.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548033.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548034.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548035.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548036.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548037.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1110.9 bits (2872), Expect = 0.0e+00
Identity = 528/565 (93.45%), Postives = 552/565 (97.70%), Query Frame = 0

Query: 1   MSDLATPLRPKRKKVWVDYFVQFRWILVVFVVLPVSFSLYFFTYLGDVRSAWKSYKQRQK 60
           MSDL  PLRPKRKKVWVDYFVQFRWILV+FVVLP+SFSLYFFTYLGDV+SAWKSY++RQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVKSAWKSYEKRQK 60

Query: 61  EHEENVKKIAKRLKERNPAKDGLVCTARKPWIAVGMRNVDYKRARHFGVDLSAFRNILEI 120
           EH+ENVKK+ KRLK+RNP KDGLVCTARKPWIAVGMRNVDYKRARHF VDLSAFRNIL+I
Sbjct: 61  EHDENVKKVVKRLKQRNPTKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKDRMIAKVEPLVNMGQISRATLPLNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DK+RMIAKVEPLVNMGQISRAT+PLNLSLAVVAELDDLTVGGLINGYGIEGSSH+YGLFS
Sbjct: 121 DKERMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYT 240
           DTVVAYEIVLADG+VVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTY 
Sbjct: 181 DTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PFIGTLKEIGQAYIDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKR 300
           PFIGTL+EIGQAY+DSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKK+
Sbjct: 241 PFIGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKK 300

Query: 301 GNVINSIGWWFKPWFYQHAATALKRGPFVEYIPTREYYHRHTRCLYWEGKLILPFADQWW 360
           GNVIN IGWWFKPWFYQHAATALK+G FVEYIPTR+YYHRHTR LYWEGKLILP ADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHAATALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPLADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIIQDMLVPLYKVPDALEWAHRELEVYPI 420
           FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMH+IQDMLVPLYKVPDAL+WAHRELEVYPI
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALDWAHRELEVYPI 420

Query: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTPYAQMYTDIGIYYAPGPVLRGEVFDGAGA 480
           WLCPHRLFKLPVKTMIYPEPGFELHRRQGDT YAQMYTD+G+YYAPGPVLRGEVFDGA A
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYNQCRKKYGAVGTFMTVYYKSKKG 540
           VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELY QCRKKYGAVGTFMTVYYK+KKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKTKKG 540

Query: 541 RKTEKEVKEAEQAHLETTYAELDQP 566
           RKTEKEVKEAEQAHLETTYAEL+QP
Sbjct: 541 RKTEKEVKEAEQAHLETTYAELEQP 565

BLAST of Sgr029579 vs. NCBI nr
Match: XP_038898378.1 (delta(24)-sterol reductase [Benincasa hispida] >XP_038898379.1 delta(24)-sterol reductase [Benincasa hispida] >XP_038898380.1 delta(24)-sterol reductase [Benincasa hispida] >XP_038898381.1 delta(24)-sterol reductase [Benincasa hispida] >XP_038898382.1 delta(24)-sterol reductase [Benincasa hispida])

HSP 1 Score: 1109.4 bits (2868), Expect = 0.0e+00
Identity = 529/565 (93.63%), Postives = 550/565 (97.35%), Query Frame = 0

Query: 1   MSDLATPLRPKRKKVWVDYFVQFRWILVVFVVLPVSFSLYFFTYLGDVRSAWKSYKQRQK 60
           MSDL  PLRPKRKKVWVDYFVQFRWILV+FVVLP+SFSLYFFTYLGDVRSAWKSYK+RQK
Sbjct: 1   MSDLVVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK 60

Query: 61  EHEENVKKIAKRLKERNPAKDGLVCTARKPWIAVGMRNVDYKRARHFGVDLSAFRNILEI 120
           EH+ENV+K+ KRLK+RNPAKDGLVCTARKPWIAVGMRNVDYKRARHF VDLSAFRNIL+I
Sbjct: 61  EHDENVQKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKDRMIAKVEPLVNMGQISRATLPLNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DKDRMIAKVEPLVNMGQISRAT+PLNLSLAVVAELDDLTVGGLINGYGIEGSSH+YGLFS
Sbjct: 121 DKDRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYT 240
           DTVVAYEIVLADG+VVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTY 
Sbjct: 181 DTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PFIGTLKEIGQAYIDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKR 300
           PF GTLKEIGQAY+DSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKR
Sbjct: 241 PFRGTLKEIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKR 300

Query: 301 GNVINSIGWWFKPWFYQHAATALKRGPFVEYIPTREYYHRHTRCLYWEGKLILPFADQWW 360
           GNVIN IGWWFKPWFYQHA+TALK+G FVEYIPTR+YYHRHTR LYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIIQDMLVPLYKVPDALEWAHRELEVYPI 420
           FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMH+IQDMLVPLYKVPDALEWAHRE EVYPI
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPI 420

Query: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTPYAQMYTDIGIYYAPGPVLRGEVFDGAGA 480
           WLCPHR+FKLPVKTMIYPEPGFEL RRQGDT YAQMYTD+G+YYAPGPVLRGEVFDGA A
Sbjct: 421 WLCPHRIFKLPVKTMIYPEPGFELQRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYNQCRKKYGAVGTFMTVYYKSKKG 540
           VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDA LY QCRKKYGAVGTFMTVYYKSKKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAGLYEQCRKKYGAVGTFMTVYYKSKKG 540

Query: 541 RKTEKEVKEAEQAHLETTYAELDQP 566
           RKTEKEV+EAEQAHLETTYAEL+QP
Sbjct: 541 RKTEKEVREAEQAHLETTYAELEQP 565

BLAST of Sgr029579 vs. NCBI nr
Match: XP_022953594.1 (delta(24)-sterol reductase [Cucurbita moschata] >XP_022953595.1 delta(24)-sterol reductase [Cucurbita moschata] >XP_022953596.1 delta(24)-sterol reductase [Cucurbita moschata] >XP_022953597.1 delta(24)-sterol reductase [Cucurbita moschata])

HSP 1 Score: 1106.7 bits (2861), Expect = 0.0e+00
Identity = 526/565 (93.10%), Postives = 551/565 (97.52%), Query Frame = 0

Query: 1   MSDLATPLRPKRKKVWVDYFVQFRWILVVFVVLPVSFSLYFFTYLGDVRSAWKSYKQRQK 60
           MSDL  PLRPKRKKVWVDYFVQFRWILV+FVVLP+SFSLYFFTYLGDV+SAWKSY++RQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVKSAWKSYEKRQK 60

Query: 61  EHEENVKKIAKRLKERNPAKDGLVCTARKPWIAVGMRNVDYKRARHFGVDLSAFRNILEI 120
           EH+ENVKK+ KRLK+RNP KDGLVCTARKPWIAVGMRNVDYKRARHF VDLSAFRNIL+I
Sbjct: 61  EHDENVKKVVKRLKQRNPTKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKDRMIAKVEPLVNMGQISRATLPLNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DK+RMIAKVEPLVNMGQISRAT+PLNLSLAVVAELDDLTVGGLINGYGIEGSSH+YGLFS
Sbjct: 121 DKERMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYT 240
           DTVVAYEIVLADG++VRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTY 
Sbjct: 181 DTVVAYEIVLADGQLVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PFIGTLKEIGQAYIDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKR 300
           PFIGTL+EIGQAY+DSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVM+GRYASKEEAKK+
Sbjct: 241 PFIGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMSGRYASKEEAKKK 300

Query: 301 GNVINSIGWWFKPWFYQHAATALKRGPFVEYIPTREYYHRHTRCLYWEGKLILPFADQWW 360
           GNVIN IGWWFKPWFYQHAATALK+G FVEYIPTR+YYHRHTR LYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHAATALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIIQDMLVPLYKVPDALEWAHRELEVYPI 420
           FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMH+IQDMLVPLYKVPDALEWAHRE EVYPI
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPI 420

Query: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTPYAQMYTDIGIYYAPGPVLRGEVFDGAGA 480
           WLCPHRLFKLPVKTMIYPEPGFELHRRQGDT YAQMYTD+G+YYAPGPVLRGEVFDGA A
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYNQCRKKYGAVGTFMTVYYKSKKG 540
           VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELY QCRKKYGAVGTFMTVYYK+KKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKTKKG 540

Query: 541 RKTEKEVKEAEQAHLETTYAELDQP 566
           RKTEKEVKEAEQAHLETT AEL+QP
Sbjct: 541 RKTEKEVKEAEQAHLETTCAELEQP 565

BLAST of Sgr029579 vs. NCBI nr
Match: XP_022991438.1 (delta(24)-sterol reductase [Cucurbita maxima] >XP_022991439.1 delta(24)-sterol reductase [Cucurbita maxima] >XP_022991440.1 delta(24)-sterol reductase [Cucurbita maxima] >XP_022991441.1 delta(24)-sterol reductase [Cucurbita maxima] >XP_022991442.1 delta(24)-sterol reductase [Cucurbita maxima] >XP_022991443.1 delta(24)-sterol reductase [Cucurbita maxima] >XP_022991444.1 delta(24)-sterol reductase [Cucurbita maxima] >XP_022991445.1 delta(24)-sterol reductase [Cucurbita maxima])

HSP 1 Score: 1106.7 bits (2861), Expect = 0.0e+00
Identity = 526/565 (93.10%), Postives = 551/565 (97.52%), Query Frame = 0

Query: 1   MSDLATPLRPKRKKVWVDYFVQFRWILVVFVVLPVSFSLYFFTYLGDVRSAWKSYKQRQK 60
           MSDL  PLRPKRKKVWVDYFVQFRWILV+FVVLP+SFSLYFFTYLGDV+SAWKSY++RQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVKSAWKSYEKRQK 60

Query: 61  EHEENVKKIAKRLKERNPAKDGLVCTARKPWIAVGMRNVDYKRARHFGVDLSAFRNILEI 120
           EH+ENVKK+ KRLK+RNP KDGLVCTARKPWIAVGMRNVDYKRARHF VDLSAFRNIL+I
Sbjct: 61  EHDENVKKVVKRLKQRNPTKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKDRMIAKVEPLVNMGQISRATLPLNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DK+RMIAKVEPLVNMGQISRAT+PLNLSLAVVAELDDLTVGGLINGYGIEGSSH+YGLFS
Sbjct: 121 DKERMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYT 240
           DTVVAYEIVLA+G+VVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTY 
Sbjct: 181 DTVVAYEIVLANGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PFIGTLKEIGQAYIDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKR 300
           PFIGTL+EIGQAY+DSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVM GRYASKEEAK +
Sbjct: 241 PFIGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMKGRYASKEEAKIK 300

Query: 301 GNVINSIGWWFKPWFYQHAATALKRGPFVEYIPTREYYHRHTRCLYWEGKLILPFADQWW 360
           GNVIN IGWWFKPWFYQHAATALK+G FVEY+PTR+YYHRHTR LYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHAATALKKGEFVEYVPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIIQDMLVPLYKVPDALEWAHRELEVYPI 420
           FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMH+IQDMLVPLYKVPDALEWAHRELEVYPI
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPI 420

Query: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTPYAQMYTDIGIYYAPGPVLRGEVFDGAGA 480
           WLCPHRLFKLPVKTMIYPEPGFELHRRQGDT YAQMYTD+G+YYAPGPVLRGEVFDGA A
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYNQCRKKYGAVGTFMTVYYKSKKG 540
           VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELY QCRKKYGAVGTFMTVYYK+KKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKTKKG 540

Query: 541 RKTEKEVKEAEQAHLETTYAELDQP 566
           RKTEKEVKEAEQAHLETTYAEL+QP
Sbjct: 541 RKTEKEVKEAEQAHLETTYAELEQP 565

BLAST of Sgr029579 vs. ExPASy Swiss-Prot
Match: P93472 (Delta(24)-sterol reductase OS=Pisum sativum OX=3888 GN=DIM PE=1 SV=1)

HSP 1 Score: 1000.0 bits (2584), Expect = 1.1e-290
Identity = 465/565 (82.30%), Postives = 520/565 (92.04%), Query Frame = 0

Query: 1   MSDLATPLRPKRKKVWVDYFVQFRWILVVFVVLPVSFSLYFFTYLGDVRSAWKSYKQRQK 60
           MSDL  PLRPKRKK+WVDYFV+FRWILV+FVVLP+SF+LYF TYLGDVRS WKS+K RQK
Sbjct: 1   MSDLEAPLRPKRKKIWVDYFVKFRWILVIFVVLPISFTLYFLTYLGDVRSEWKSFKTRQK 60

Query: 61  EHEENVKKIAKRLKERNPAKDGLVCTARKPWIAVGMRNVDYKRARHFGVDLSAFRNILEI 120
           EH+ENV+K+  RLK+RNP+KDGLVCTARKPW+AVGMRNVDYKRARHF VDLS FRNIL+I
Sbjct: 61  EHDENVQKVVNRLKKRNPSKDGLVCTARKPWVAVGMRNVDYKRARHFEVDLSPFRNILDI 120

Query: 121 DKDRMIAKVEPLVNMGQISRATLPLNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DK+RMIA+VEPLVNMGQI+R T+P+NL+LAVVAELDDLTVGGLINGYGIEGSSH YGLFS
Sbjct: 121 DKERMIARVEPLVNMGQITRVTVPMNLALAVVAELDDLTVGGLINGYGIEGSSHKYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYT 240
           DTVVA+EI+LADG +V+ATKDNEYSDLFYAIPWSQGT+GLLVAAE+KLIPIKEYMK+TY 
Sbjct: 181 DTVVAFEIILADGSLVKATKDNEYSDLFYAIPWSQGTLGLLVAAEVKLIPIKEYMKLTYK 240

Query: 241 PFIGTLKEIGQAYIDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKR 300
           P +G LK+I QAY DS APRDGDQDN EKVPDFVETMIY+P+ AV MTGRYASKEEAKK+
Sbjct: 241 PVVGNLKDIAQAYSDSFAPRDGDQDNDEKVPDFVETMIYSPTRAVCMTGRYASKEEAKKK 300

Query: 301 GNVINSIGWWFKPWFYQHAATALKRGPFVEYIPTREYYHRHTRCLYWEGKLILPFADQWW 360
           GN IN++GWW+K WFYQHA TALK+G FVEYIPTREYYHRHTRCLYWEGKLILPF DQ+ 
Sbjct: 301 GNKINNVGWWYKTWFYQHAETALKKGLFVEYIPTREYYHRHTRCLYWEGKLILPFGDQFS 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIIQDMLVPLYKVPDALEWAHRELEVYPI 420
           FRFL GW+MPPKVSLLKATQGEAIRNYYHEMH+IQDMLVPLYKV DALEW+ RE+E+YPI
Sbjct: 361 FRFLFGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVGDALEWSDREMEIYPI 420

Query: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTPYAQMYTDIGIYYAPGPVLRGEVFDGAGA 480
           WLCPH+LFKLP+KTMIYPE GFEL RRQGDT  AQM+TD+G+YYAPGPVLRGEVFDGA A
Sbjct: 421 WLCPHKLFKLPIKTMIYPEAGFELQRRQGDTQNAQMFTDVGVYYAPGPVLRGEVFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYNQCRKKYGAVGTFMTVYYKSKKG 540
           VR+ME W++ENH FQ  YAVSELNEK+FWRMFDA LY  CR+KYGAVGTFM+VYYK KKG
Sbjct: 481 VRKMESWMIENHCFQPQYAVSELNEKNFWRMFDAGLYEHCRRKYGAVGTFMSVYYKCKKG 540

Query: 541 RKTEKEVKEAEQAHLETTYAELDQP 566
           RKTEKEV+EAEQAHL+T Y E+DQP
Sbjct: 541 RKTEKEVREAEQAHLDTAYPEVDQP 565

BLAST of Sgr029579 vs. ExPASy Swiss-Prot
Match: Q39085 (Delta(24)-sterol reductase OS=Arabidopsis thaliana OX=3702 GN=DIM PE=1 SV=2)

HSP 1 Score: 939.9 bits (2428), Expect = 1.4e-272
Identity = 442/564 (78.37%), Postives = 500/564 (88.65%), Query Frame = 0

Query: 1   MSDLATPL-RPKRKKVWVDYFVQFRWILVVFVVLPVSFSLYFFTYLGDVRSAWKSYKQRQ 60
           MSDL TPL RPKRKK WVDYFV+FRWI+V+F+VLP S + YF  YLGD+ S  KS+++RQ
Sbjct: 1   MSDLQTPLVRPKRKKTWVDYFVKFRWIIVIFIVLPFSATFYFLIYLGDMWSESKSFEKRQ 60

Query: 61  KEHEENVKKIAKRLKERNPAKDGLVCTARKPWIAVGMRNVDYKRARHFGVDLSAFRNILE 120
           KEH+ENVKK+ KRLK R+ +KDGLVCTARKPWIAVGMRNVDYKRARHF VDL  FRNILE
Sbjct: 61  KEHDENVKKVIKRLKGRDASKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLGEFRNILE 120

Query: 121 IDKDRMIAKVEPLVNMGQISRATLPLNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180
           I+K++M A+VEPLVNMGQISRAT+P+NLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF
Sbjct: 121 INKEKMTARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180

Query: 181 SDTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTY 240
           +DTV AYEIVLA G +VRAT+DNEYSDL+YAIPWSQGT+GLLVAAEI+LI +KEYM++TY
Sbjct: 181 ADTVEAYEIVLAGGELVRATRDNEYSDLYYAIPWSQGTLGLLVAAEIRLIKVKEYMRLTY 240

Query: 241 TPFIGTLKEIGQAYIDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKK 300
            P  G L+ + Q YIDS AP+DGD+    K+PDFVE M+Y P+E V+M G YASKEEAKK
Sbjct: 241 IPVKGDLQALAQGYIDSFAPKDGDK---SKIPDFVEGMVYNPTEGVMMVGTYASKEEAKK 300

Query: 301 RGNVINSIGWWFKPWFYQHAATALKRGPFVEYIPTREYYHRHTRCLYWEGKLILPFADQW 360
           +GN IN++GWWFKPWFYQHA TALK+G FVEYIPTREYYHRHTRCLYWEGKLILPF DQ+
Sbjct: 301 KGNKINNVGWWFKPWFYQHAQTALKKGQFVEYIPTREYYHRHTRCLYWEGKLILPFGDQF 360

Query: 361 WFRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIIQDMLVPLYKVPDALEWAHRELEVYP 420
           WFR+LLGW+MPPKVSLLKATQGEAIRNYYH+MH+IQDMLVPLYKV DALEW HRE+EVYP
Sbjct: 361 WFRYLLGWLMPPKVSLLKATQGEAIRNYYHDMHVIQDMLVPLYKVGDALEWVHREMEVYP 420

Query: 421 IWLCPHRLFKLPVKTMIYPEPGFELHRRQGDTPYAQMYTDIGIYYAPGPVLRGEVFDGAG 480
           IWLCPH+LFK P+K  IYPEPGFE   RQGDT  AQMYTD+G+YYAPG VLRGE FDG+ 
Sbjct: 421 IWLCPHKLFKQPIKGQIYPEPGFEYENRQGDTEDAQMYTDVGVYYAPGCVLRGEEFDGSE 480

Query: 481 AVRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYNQCRKKYGAVGTFMTVYYKSKK 540
           AVRRMEKWL+ENHGFQ  YAVSEL+EK FWRMF+ ELY +CRKKY A+GTFM+VYYKSKK
Sbjct: 481 AVRRMEKWLIENHGFQPQYAVSELDEKSFWRMFNGELYEECRKKYRAIGTFMSVYYKSKK 540

Query: 541 GRKTEKEVKEAEQAHLETTYAELD 564
           GRKTEKEV+EAEQAHLET YAE D
Sbjct: 541 GRKTEKEVREAEQAHLETAYAEAD 561

BLAST of Sgr029579 vs. ExPASy Swiss-Prot
Match: Q15392 (Delta(24)-sterol reductase OS=Homo sapiens OX=9606 GN=DHCR24 PE=1 SV=2)

HSP 1 Score: 384.0 bits (985), Expect = 2.9e-105
Identity = 220/525 (41.90%), Postives = 309/525 (58.86%), Query Frame = 0

Query: 24  RWILVVFVVLPVS--FSLYFFTYLGDVRSAWKSYK--QRQKEHEENVKKIAKRLKE-RNP 83
           RW+ V   +LP+S  F +Y++        AW  +K     + HE+ V+ I K+++E +  
Sbjct: 31  RWVFVCLFLLPLSLIFDIYYYV------RAWVVFKLSSAPRLHEQRVRDIQKQVREWKEQ 90

Query: 84  AKDGLVCTARKPWIAVGMRNVDYKRARHFGVDLSAFRNILEIDKDRMIAKVEPLVNMGQI 143
                +CT R  W+ V +R   YK+  H  + ++   +ILE+D  + I +VEPLV MGQ+
Sbjct: 91  GSKTFMCTGRPGWLTVSLRVGKYKKT-HKNIMIN-LMDILEVDTKKQIVRVEPLVTMGQV 150

Query: 144 SRATLPLNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFSDTVVAYEIVLADGRVVRA 203
           +     +  +L V+ ELDDLTVGGLI G GIE SSH YGLF     AYE+VLADG  VR 
Sbjct: 151 TALLTSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELVLADGSFVRC 210

Query: 204 TKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYTPFIGTLKEIGQAYIDSLA 263
           T  +E SDLFYA+PWS GT+G LVAAEI++IP K+Y+K+ + P  G      +A      
Sbjct: 211 T-PSENSDLFYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVRGL-----EAICAKFT 270

Query: 264 PRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKRGNVINSIGWWFKPWFYQH 323
                Q+N      FVE ++Y+  EAV+MTG    + E  K    +NSIG ++KPWF++H
Sbjct: 271 HESQRQEN-----HFVEGLLYSLDEAVIMTGVMTDEAEPSK----LNSIGNYYKPWFFKH 330

Query: 324 AATALKRG-PFVEYIPTREYYHRHTRCLYWEGKLILPFADQWWFRFLLGWMMPPKVSLLK 383
               LK     +EYIP R YYHRHTR ++WE + I+PF +   FR+L GWM+PPK+SLLK
Sbjct: 331 VENYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMVPPKISLLK 390

Query: 384 ATQGEAIRNYYHEMHIIQDMLVPLYKVPDALEWAHRELEVYPIWLCPHRLFKLPVKTMIY 443
            TQGE +R  Y + H++QDMLVP+  +  AL     ++ VYPIWLCP   F LP      
Sbjct: 391 LTQGETLRKLYEQHHVVQDMLVPMKCLQQALHTFQNDIHVYPIWLCP---FILP------ 450

Query: 444 PEPGFELHRRQGDTPYAQMYTDIGIYYAPGPVLRGEVFDGAGAVRRMEKWLLENHGFQAL 503
            +PG  +H +  +   A++Y DIG Y  P    R + F+    +R++EK++   HGFQ L
Sbjct: 451 SQPGL-VHPKGNE---AELYIDIGAYGEP----RVKHFEARSCMRQLEKFVRSVHGFQML 510

Query: 504 YAVSELNEKDFWRMFDAELYNQCRKKYGAVGTFMTVYYKSKKGRK 543
           YA   +N ++FW MFD  LY++ R+K G    F  VY K  K  +
Sbjct: 511 YADCYMNREEFWEMFDGSLYHKLREKLGCQDAFPEVYDKICKAAR 515

BLAST of Sgr029579 vs. ExPASy Swiss-Prot
Match: Q60HC5 (Delta(24)-sterol reductase OS=Macaca fascicularis OX=9541 GN=DHCR24 PE=2 SV=2)

HSP 1 Score: 383.6 bits (984), Expect = 3.7e-105
Identity = 220/525 (41.90%), Postives = 309/525 (58.86%), Query Frame = 0

Query: 24  RWILVVFVVLPVS--FSLYFFTYLGDVRSAWKSYK--QRQKEHEENVKKIAKRLKE-RNP 83
           RW+ V   +LP+S  F +Y++        AW  +K     + HE+ V+ I K+++E +  
Sbjct: 31  RWVFVCLFLLPLSLIFDIYYYV------RAWVVFKLSSAPRLHEQRVRDIQKQVREWKEQ 90

Query: 84  AKDGLVCTARKPWIAVGMRNVDYKRARHFGVDLSAFRNILEIDKDRMIAKVEPLVNMGQI 143
                +CT R  W+ V +R   YK+  H  + ++   +ILE+D  + I +VEPLV MGQ+
Sbjct: 91  GSKTFMCTGRPGWLTVSLRVGKYKKT-HKNIMIN-LMDILEVDTKKQIVRVEPLVTMGQV 150

Query: 144 SRATLPLNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFSDTVVAYEIVLADGRVVRA 203
           +     +  +L V+ ELDDLTVGGLI G GIE SSH YGLF     AYE+VLADG  VR 
Sbjct: 151 TALLTSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELVLADGSFVRC 210

Query: 204 TKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYTPFIGTLKEIGQAYIDSLA 263
           T  +E SDLFYA+PWS GT+G LVAAEI++IP K+Y+K+ + P  G      +A      
Sbjct: 211 T-PSENSDLFYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVQGL-----EAICAKFT 270

Query: 264 PRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKRGNVINSIGWWFKPWFYQH 323
                Q+N      FVE ++Y+  EAV+MTG    + E  K    +NSIG ++KPWF++H
Sbjct: 271 HESQRQEN-----HFVEGLLYSLDEAVIMTGVMTDEVEPSK----LNSIGNYYKPWFFKH 330

Query: 324 AATALKRG-PFVEYIPTREYYHRHTRCLYWEGKLILPFADQWWFRFLLGWMMPPKVSLLK 383
               LK     +EYIP R YYHRHTR ++WE + I+PF +   FR+L GWM+PPK+SLLK
Sbjct: 331 VENYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMVPPKISLLK 390

Query: 384 ATQGEAIRNYYHEMHIIQDMLVPLYKVPDALEWAHRELEVYPIWLCPHRLFKLPVKTMIY 443
            TQGE +R  Y + H++QDMLVP+  +  AL     ++ VYPIWLCP   F LP      
Sbjct: 391 LTQGETLRKLYEQHHVVQDMLVPMKCLQQALHTFQNDIHVYPIWLCP---FILP------ 450

Query: 444 PEPGFELHRRQGDTPYAQMYTDIGIYYAPGPVLRGEVFDGAGAVRRMEKWLLENHGFQAL 503
            +PG  +H +  +   A++Y DIG Y  P    R + F+    +R++EK++   HGFQ L
Sbjct: 451 SQPGL-VHPKGNE---AELYIDIGAYGEP----RVKHFEARSCMRQLEKFVRSVHGFQML 510

Query: 504 YAVSELNEKDFWRMFDAELYNQCRKKYGAVGTFMTVYYKSKKGRK 543
           YA   +N ++FW MFD  LY++ R+K G    F  VY K  K  +
Sbjct: 511 YADCYMNREEFWEMFDGSLYHKLREKLGCQDAFPEVYDKICKAAR 515

BLAST of Sgr029579 vs. ExPASy Swiss-Prot
Match: Q8VCH6 (Delta(24)-sterol reductase OS=Mus musculus OX=10090 GN=Dhcr24 PE=1 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 1.1e-104
Identity = 220/525 (41.90%), Postives = 312/525 (59.43%), Query Frame = 0

Query: 24  RWILVVFVVLPVS--FSLYFFTYLGDVRSAWKSYK--QRQKEHEENVKKIAKRLKE-RNP 83
           RW+ V   +LP+S  F +Y++        AW  +K     + HE+ V+ I K+++E +  
Sbjct: 31  RWVFVCLFLLPLSLIFDIYYYV------RAWVVFKLSSAPRLHEQRVRDIQKQVREWKEQ 90

Query: 84  AKDGLVCTARKPWIAVGMRNVDYKRARHFGVDLSAFRNILEIDKDRMIAKVEPLVNMGQI 143
                +CT R  W+ V +R   YK+  H  + ++   +ILE+D  + I +VEPLV+MGQ+
Sbjct: 91  GSKTFMCTGRPGWLTVSLRVGKYKKT-HKNIMIN-LMDILEVDTKKQIVRVEPLVSMGQV 150

Query: 144 SRATLPLNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFSDTVVAYEIVLADGRVVRA 203
           +     +  +L V+ ELDDLTVGGLI G GIE SSH YGLF     AYE++LADG  VR 
Sbjct: 151 TALLNSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELILADGSFVRC 210

Query: 204 TKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYTPFIGTLKEIGQAYIDSLA 263
           T  +E SDLFYA+PWS GT+G LVAAEI++IP K+Y+K+ + P  G L+ I + +     
Sbjct: 211 T-PSENSDLFYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVRG-LEAICEKF----- 270

Query: 264 PRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKRGNVINSIGWWFKPWFYQH 323
                +++      FVE ++Y+  EAV+MTG      E  K    +NSIG ++KPWF++H
Sbjct: 271 ----TRESQRLENHFVEGLLYSLDEAVIMTGVMTDDVEPSK----LNSIGSYYKPWFFKH 330

Query: 324 AATALKRG-PFVEYIPTREYYHRHTRCLYWEGKLILPFADQWWFRFLLGWMMPPKVSLLK 383
               LK     +EYIP R YYHRHTR ++WE + I+PF +   FR+L GWM+PPK+SLLK
Sbjct: 331 VENYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMVPPKISLLK 390

Query: 384 ATQGEAIRNYYHEMHIIQDMLVPLYKVPDALEWAHRELEVYPIWLCPHRLFKLPVKTMIY 443
            TQGE +R  Y + H++QDMLVP+  +  AL     ++ VYPIWLCP   F LP      
Sbjct: 391 LTQGETLRKLYEQHHVVQDMLVPMKCMSQALHTFQNDIHVYPIWLCP---FILP------ 450

Query: 444 PEPGFELHRRQGDTPYAQMYTDIGIYYAPGPVLRGEVFDGAGAVRRMEKWLLENHGFQAL 503
            +PG  L   +GD   A++Y DIG Y  P    R + F+    +R++EK++   HGFQ L
Sbjct: 451 SQPG--LVHPKGDE--AELYVDIGAYGEP----RVKHFEARSCMRQLEKFVRSVHGFQML 510

Query: 504 YAVSELNEKDFWRMFDAELYNQCRKKYGAVGTFMTVYYKSKKGRK 543
           YA   +N ++FW MFD  LY++ RK+ G    F  VY K  K  +
Sbjct: 511 YADCYMNREEFWEMFDGSLYHKLRKQLGCQDAFPEVYDKICKAAR 515

BLAST of Sgr029579 vs. ExPASy TrEMBL
Match: A0A6J1JUW6 (Delta(24)-sterol reductase OS=Cucurbita maxima OX=3661 GN=LOC111488060 PE=4 SV=1)

HSP 1 Score: 1106.7 bits (2861), Expect = 0.0e+00
Identity = 526/565 (93.10%), Postives = 551/565 (97.52%), Query Frame = 0

Query: 1   MSDLATPLRPKRKKVWVDYFVQFRWILVVFVVLPVSFSLYFFTYLGDVRSAWKSYKQRQK 60
           MSDL  PLRPKRKKVWVDYFVQFRWILV+FVVLP+SFSLYFFTYLGDV+SAWKSY++RQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVKSAWKSYEKRQK 60

Query: 61  EHEENVKKIAKRLKERNPAKDGLVCTARKPWIAVGMRNVDYKRARHFGVDLSAFRNILEI 120
           EH+ENVKK+ KRLK+RNP KDGLVCTARKPWIAVGMRNVDYKRARHF VDLSAFRNIL+I
Sbjct: 61  EHDENVKKVVKRLKQRNPTKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKDRMIAKVEPLVNMGQISRATLPLNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DK+RMIAKVEPLVNMGQISRAT+PLNLSLAVVAELDDLTVGGLINGYGIEGSSH+YGLFS
Sbjct: 121 DKERMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYT 240
           DTVVAYEIVLA+G+VVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTY 
Sbjct: 181 DTVVAYEIVLANGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PFIGTLKEIGQAYIDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKR 300
           PFIGTL+EIGQAY+DSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVM GRYASKEEAK +
Sbjct: 241 PFIGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMKGRYASKEEAKIK 300

Query: 301 GNVINSIGWWFKPWFYQHAATALKRGPFVEYIPTREYYHRHTRCLYWEGKLILPFADQWW 360
           GNVIN IGWWFKPWFYQHAATALK+G FVEY+PTR+YYHRHTR LYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHAATALKKGEFVEYVPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIIQDMLVPLYKVPDALEWAHRELEVYPI 420
           FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMH+IQDMLVPLYKVPDALEWAHRELEVYPI
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPI 420

Query: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTPYAQMYTDIGIYYAPGPVLRGEVFDGAGA 480
           WLCPHRLFKLPVKTMIYPEPGFELHRRQGDT YAQMYTD+G+YYAPGPVLRGEVFDGA A
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYNQCRKKYGAVGTFMTVYYKSKKG 540
           VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELY QCRKKYGAVGTFMTVYYK+KKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKTKKG 540

Query: 541 RKTEKEVKEAEQAHLETTYAELDQP 566
           RKTEKEVKEAEQAHLETTYAEL+QP
Sbjct: 541 RKTEKEVKEAEQAHLETTYAELEQP 565

BLAST of Sgr029579 vs. ExPASy TrEMBL
Match: A0A6J1GQ50 (Delta(24)-sterol reductase OS=Cucurbita moschata OX=3662 GN=LOC111456086 PE=4 SV=1)

HSP 1 Score: 1106.7 bits (2861), Expect = 0.0e+00
Identity = 526/565 (93.10%), Postives = 551/565 (97.52%), Query Frame = 0

Query: 1   MSDLATPLRPKRKKVWVDYFVQFRWILVVFVVLPVSFSLYFFTYLGDVRSAWKSYKQRQK 60
           MSDL  PLRPKRKKVWVDYFVQFRWILV+FVVLP+SFSLYFFTYLGDV+SAWKSY++RQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVKSAWKSYEKRQK 60

Query: 61  EHEENVKKIAKRLKERNPAKDGLVCTARKPWIAVGMRNVDYKRARHFGVDLSAFRNILEI 120
           EH+ENVKK+ KRLK+RNP KDGLVCTARKPWIAVGMRNVDYKRARHF VDLSAFRNIL+I
Sbjct: 61  EHDENVKKVVKRLKQRNPTKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKDRMIAKVEPLVNMGQISRATLPLNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DK+RMIAKVEPLVNMGQISRAT+PLNLSLAVVAELDDLTVGGLINGYGIEGSSH+YGLFS
Sbjct: 121 DKERMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYT 240
           DTVVAYEIVLADG++VRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTY 
Sbjct: 181 DTVVAYEIVLADGQLVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PFIGTLKEIGQAYIDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKR 300
           PFIGTL+EIGQAY+DSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVM+GRYASKEEAKK+
Sbjct: 241 PFIGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMSGRYASKEEAKKK 300

Query: 301 GNVINSIGWWFKPWFYQHAATALKRGPFVEYIPTREYYHRHTRCLYWEGKLILPFADQWW 360
           GNVIN IGWWFKPWFYQHAATALK+G FVEYIPTR+YYHRHTR LYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHAATALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIIQDMLVPLYKVPDALEWAHRELEVYPI 420
           FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMH+IQDMLVPLYKVPDALEWAHRE EVYPI
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPI 420

Query: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTPYAQMYTDIGIYYAPGPVLRGEVFDGAGA 480
           WLCPHRLFKLPVKTMIYPEPGFELHRRQGDT YAQMYTD+G+YYAPGPVLRGEVFDGA A
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYNQCRKKYGAVGTFMTVYYKSKKG 540
           VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELY QCRKKYGAVGTFMTVYYK+KKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKTKKG 540

Query: 541 RKTEKEVKEAEQAHLETTYAELDQP 566
           RKTEKEVKEAEQAHLETT AEL+QP
Sbjct: 541 RKTEKEVKEAEQAHLETTCAELEQP 565

BLAST of Sgr029579 vs. ExPASy TrEMBL
Match: A0A6J1H6V4 (Delta(24)-sterol reductase OS=Cucurbita moschata OX=3662 GN=LOC111460987 PE=4 SV=1)

HSP 1 Score: 1099.0 bits (2841), Expect = 0.0e+00
Identity = 520/565 (92.04%), Postives = 549/565 (97.17%), Query Frame = 0

Query: 1   MSDLATPLRPKRKKVWVDYFVQFRWILVVFVVLPVSFSLYFFTYLGDVRSAWKSYKQRQK 60
           MSDL  PLRPKRKKVWVDYFV+FRWILV+FVVLP+SF LYFFTYLGDVRSAWKSYK+RQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQK 60

Query: 61  EHEENVKKIAKRLKERNPAKDGLVCTARKPWIAVGMRNVDYKRARHFGVDLSAFRNILEI 120
           EH+ENV+K+ KRLK+RNPAKDGLVCTARKPWIAVGMRNVDYKRARHF VDLSAFRNIL+I
Sbjct: 61  EHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKDRMIAKVEPLVNMGQISRATLPLNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           D+++MIAKVEPLVNMGQISRAT+PLNLSLAVVAELDDLTVGGLINGYGIEGSSH+YGLFS
Sbjct: 121 DQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYT 240
           DTVVAYE+VLADG+VVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTY 
Sbjct: 181 DTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PFIGTLKEIGQAYIDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKR 300
           PF GTL+EIGQAY+DSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASK EAK++
Sbjct: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRK 300

Query: 301 GNVINSIGWWFKPWFYQHAATALKRGPFVEYIPTREYYHRHTRCLYWEGKLILPFADQWW 360
           GNVIN IGWWFKPWFYQHA+TALK+G FVEYIPTR+YYHRHTR LYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIIQDMLVPLYKVPDALEWAHRELEVYPI 420
           FRFL GWMMPPKVSLLKATQGEAIRNYYHEMH+IQDMLVPLYKVPDALEWAHRELEVYP+
Sbjct: 361 FRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPV 420

Query: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTPYAQMYTDIGIYYAPGPVLRGEVFDGAGA 480
           WLCPHRLFKLPVKTMIYPEPGFELHRRQGDT YAQMYTD+G+YYAPGP+LRGEVFDGA A
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEVFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYNQCRKKYGAVGTFMTVYYKSKKG 540
           VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAE Y QCRKKYGAVGTFMTVYYKSKKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKKG 540

Query: 541 RKTEKEVKEAEQAHLETTYAELDQP 566
           RKTEKEVKEAEQAHLETTYAEL+QP
Sbjct: 541 RKTEKEVKEAEQAHLETTYAELEQP 565

BLAST of Sgr029579 vs. ExPASy TrEMBL
Match: A0A6J1KZQ6 (Delta(24)-sterol reductase OS=Cucurbita maxima OX=3661 GN=LOC111497787 PE=4 SV=1)

HSP 1 Score: 1098.6 bits (2840), Expect = 0.0e+00
Identity = 519/565 (91.86%), Postives = 549/565 (97.17%), Query Frame = 0

Query: 1   MSDLATPLRPKRKKVWVDYFVQFRWILVVFVVLPVSFSLYFFTYLGDVRSAWKSYKQRQK 60
           MSDL  PLRPKRKKVWVDYFV+FRWILV+FVVLP+SF LYFFTYLGDVRSAWKSYK+RQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQK 60

Query: 61  EHEENVKKIAKRLKERNPAKDGLVCTARKPWIAVGMRNVDYKRARHFGVDLSAFRNILEI 120
           EH+ENV+K+ KRLK+RNPAKDGLVCTARKPWIAVGMRNVDYKRARHF VDLSAFRNIL+I
Sbjct: 61  EHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKDRMIAKVEPLVNMGQISRATLPLNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           D+++MIAKVEPLVNMGQISRAT+PLNLSLAVVAELDDLTVGGLINGYGIEGSSH+YGLFS
Sbjct: 121 DQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYT 240
           DTVVAYE+VLADG+VVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTY 
Sbjct: 181 DTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PFIGTLKEIGQAYIDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKR 300
           PF GTL+EIGQAY+DSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASK EAK++
Sbjct: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRK 300

Query: 301 GNVINSIGWWFKPWFYQHAATALKRGPFVEYIPTREYYHRHTRCLYWEGKLILPFADQWW 360
           GNVIN IGWWFKPWFYQHA+TALK+G FVEYIPTR+YYHRHTR LYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIIQDMLVPLYKVPDALEWAHRELEVYPI 420
           FRFL GWMMPPKVSLLKATQGEAIRNYYHEMH+IQDMLVPLYKVPDALEWAHRELEVYP+
Sbjct: 361 FRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPV 420

Query: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTPYAQMYTDIGIYYAPGPVLRGEVFDGAGA 480
           WLCPHRLFKLPVKTMIYPEPGFELHRRQGDT YAQMYTD+G+YYAPGP+LRGE+FDGA A
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEIFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYNQCRKKYGAVGTFMTVYYKSKKG 540
           VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAE Y QCRKKYGAVGTFMTVYYKSKKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKKG 540

Query: 541 RKTEKEVKEAEQAHLETTYAELDQP 566
           RKTEKEVKEAEQAHLETTYAEL+QP
Sbjct: 541 RKTEKEVKEAEQAHLETTYAELEQP 565

BLAST of Sgr029579 vs. ExPASy TrEMBL
Match: A0A6J1DEM5 (Delta(24)-sterol reductase OS=Momordica charantia OX=3673 GN=LOC111019723 PE=4 SV=1)

HSP 1 Score: 1090.9 bits (2820), Expect = 0.0e+00
Identity = 520/568 (91.55%), Postives = 548/568 (96.48%), Query Frame = 0

Query: 1   MSDLATPLRPKRKKVWVDYFVQFRWILVVFVVLPVSFSLYFFTYLGDVRSAWKSYKQRQK 60
           MSDL TPLR KRKKVWVDYFVQFRWILVVFVVLP+SFSLYFFTYLGDVRSAWKSYK+RQK
Sbjct: 1   MSDLQTPLRRKRKKVWVDYFVQFRWILVVFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK 60

Query: 61  EHEENVKKIAKRLKERNPAKDGLVCTARKPWIAVGMRNVDYKRARHFGVDLSAFRNILEI 120
           +H+ENVKK+ KRLK+RNPAKDGLVCTARKPWIAVGMRNVDYKRARHF VDLSAFRNILEI
Sbjct: 61  QHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILEI 120

Query: 121 DKDRMIAKVEPLVNMGQISRATLPLNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           D+ RMIAKVEPLVNMGQISRAT+PLNLSLAVVAELDDLTVGGLINGYGIEGSSH+YGLFS
Sbjct: 121 DQQRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYT 240
           DTVVAYEIVLADGRVVRATKDN+YSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYM+VTY 
Sbjct: 181 DTVVAYEIVLADGRVVRATKDNKYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMRVTYK 240

Query: 241 PFIGTLKEIGQAYIDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKR 300
           P+IGTL++IGQAY+DSLAPRDGDQDNPEKVPDFVETM+YTPSEAV+M+GRYASK EAKKR
Sbjct: 241 PYIGTLRDIGQAYMDSLAPRDGDQDNPEKVPDFVETMVYTPSEAVIMSGRYASKVEAKKR 300

Query: 301 GNVINSIGWWFKPWFYQHAATALKRGPFVEYIPTREYYHRHTRCLYWEGKLILPFADQWW 360
           GNVIN+IGWWFKPWFYQHAATALKRG FVEYIPTREYYHRHTR LYWEGKLILPFADQWW
Sbjct: 301 GNVINNIGWWFKPWFYQHAATALKRGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIIQDMLVPLYKVPDALEWAHRELEVYPI 420
           FRF +GW+MPPKVSLLKATQGEAIRNYYHEMH+IQDMLVPLYKVPDALEWAHRELEVYPI
Sbjct: 361 FRFFMGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPI 420

Query: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTPYAQMYTDIGIYYAPGPVLRGEVFDGAGA 480
           WLCPHR+FKLPVKTMIYPEPGFE HRRQGDT YAQMYTD+G+YYAPGPVLRGEVFDG+ A
Sbjct: 421 WLCPHRIFKLPVKTMIYPEPGFEFHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGSEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYNQCRKKYGAVGTFMTVYYKSKKG 540
           VR +EKWLLENHGFQALYAVSELNEKDFWRMFD  LY QCRKKYGAVGTFMTVYYKSKKG
Sbjct: 481 VRGLEKWLLENHGFQALYAVSELNEKDFWRMFDGGLYEQCRKKYGAVGTFMTVYYKSKKG 540

Query: 541 RKTEKEVKEA--EQAHLETTYAELDQPQ 567
           RKTEKEV+EA  EQAH ETTYAELDQPQ
Sbjct: 541 RKTEKEVREAELEQAHHETTYAELDQPQ 568

BLAST of Sgr029579 vs. TAIR 10
Match: AT3G19820.1 (cell elongation protein / DWARF1 / DIMINUTO (DIM) )

HSP 1 Score: 939.9 bits (2428), Expect = 9.6e-274
Identity = 442/564 (78.37%), Postives = 500/564 (88.65%), Query Frame = 0

Query: 1   MSDLATPL-RPKRKKVWVDYFVQFRWILVVFVVLPVSFSLYFFTYLGDVRSAWKSYKQRQ 60
           MSDL TPL RPKRKK WVDYFV+FRWI+V+F+VLP S + YF  YLGD+ S  KS+++RQ
Sbjct: 1   MSDLQTPLVRPKRKKTWVDYFVKFRWIIVIFIVLPFSATFYFLIYLGDMWSESKSFEKRQ 60

Query: 61  KEHEENVKKIAKRLKERNPAKDGLVCTARKPWIAVGMRNVDYKRARHFGVDLSAFRNILE 120
           KEH+ENVKK+ KRLK R+ +KDGLVCTARKPWIAVGMRNVDYKRARHF VDL  FRNILE
Sbjct: 61  KEHDENVKKVIKRLKGRDASKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLGEFRNILE 120

Query: 121 IDKDRMIAKVEPLVNMGQISRATLPLNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180
           I+K++M A+VEPLVNMGQISRAT+P+NLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF
Sbjct: 121 INKEKMTARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180

Query: 181 SDTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTY 240
           +DTV AYEIVLA G +VRAT+DNEYSDL+YAIPWSQGT+GLLVAAEI+LI +KEYM++TY
Sbjct: 181 ADTVEAYEIVLAGGELVRATRDNEYSDLYYAIPWSQGTLGLLVAAEIRLIKVKEYMRLTY 240

Query: 241 TPFIGTLKEIGQAYIDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKK 300
            P  G L+ + Q YIDS AP+DGD+    K+PDFVE M+Y P+E V+M G YASKEEAKK
Sbjct: 241 IPVKGDLQALAQGYIDSFAPKDGDK---SKIPDFVEGMVYNPTEGVMMVGTYASKEEAKK 300

Query: 301 RGNVINSIGWWFKPWFYQHAATALKRGPFVEYIPTREYYHRHTRCLYWEGKLILPFADQW 360
           +GN IN++GWWFKPWFYQHA TALK+G FVEYIPTREYYHRHTRCLYWEGKLILPF DQ+
Sbjct: 301 KGNKINNVGWWFKPWFYQHAQTALKKGQFVEYIPTREYYHRHTRCLYWEGKLILPFGDQF 360

Query: 361 WFRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIIQDMLVPLYKVPDALEWAHRELEVYP 420
           WFR+LLGW+MPPKVSLLKATQGEAIRNYYH+MH+IQDMLVPLYKV DALEW HRE+EVYP
Sbjct: 361 WFRYLLGWLMPPKVSLLKATQGEAIRNYYHDMHVIQDMLVPLYKVGDALEWVHREMEVYP 420

Query: 421 IWLCPHRLFKLPVKTMIYPEPGFELHRRQGDTPYAQMYTDIGIYYAPGPVLRGEVFDGAG 480
           IWLCPH+LFK P+K  IYPEPGFE   RQGDT  AQMYTD+G+YYAPG VLRGE FDG+ 
Sbjct: 421 IWLCPHKLFKQPIKGQIYPEPGFEYENRQGDTEDAQMYTDVGVYYAPGCVLRGEEFDGSE 480

Query: 481 AVRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYNQCRKKYGAVGTFMTVYYKSKK 540
           AVRRMEKWL+ENHGFQ  YAVSEL+EK FWRMF+ ELY +CRKKY A+GTFM+VYYKSKK
Sbjct: 481 AVRRMEKWLIENHGFQPQYAVSELDEKSFWRMFNGELYEECRKKYRAIGTFMSVYYKSKK 540

Query: 541 GRKTEKEVKEAEQAHLETTYAELD 564
           GRKTEKEV+EAEQAHLET YAE D
Sbjct: 541 GRKTEKEVREAEQAHLETAYAEAD 561

BLAST of Sgr029579 vs. TAIR 10
Match: AT3G19820.2 (cell elongation protein / DWARF1 / DIMINUTO (DIM) )

HSP 1 Score: 939.9 bits (2428), Expect = 9.6e-274
Identity = 442/564 (78.37%), Postives = 500/564 (88.65%), Query Frame = 0

Query: 1   MSDLATPL-RPKRKKVWVDYFVQFRWILVVFVVLPVSFSLYFFTYLGDVRSAWKSYKQRQ 60
           MSDL TPL RPKRKK WVDYFV+FRWI+V+F+VLP S + YF  YLGD+ S  KS+++RQ
Sbjct: 1   MSDLQTPLVRPKRKKTWVDYFVKFRWIIVIFIVLPFSATFYFLIYLGDMWSESKSFEKRQ 60

Query: 61  KEHEENVKKIAKRLKERNPAKDGLVCTARKPWIAVGMRNVDYKRARHFGVDLSAFRNILE 120
           KEH+ENVKK+ KRLK R+ +KDGLVCTARKPWIAVGMRNVDYKRARHF VDL  FRNILE
Sbjct: 61  KEHDENVKKVIKRLKGRDASKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLGEFRNILE 120

Query: 121 IDKDRMIAKVEPLVNMGQISRATLPLNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180
           I+K++M A+VEPLVNMGQISRAT+P+NLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF
Sbjct: 121 INKEKMTARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180

Query: 181 SDTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTY 240
           +DTV AYEIVLA G +VRAT+DNEYSDL+YAIPWSQGT+GLLVAAEI+LI +KEYM++TY
Sbjct: 181 ADTVEAYEIVLAGGELVRATRDNEYSDLYYAIPWSQGTLGLLVAAEIRLIKVKEYMRLTY 240

Query: 241 TPFIGTLKEIGQAYIDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKK 300
            P  G L+ + Q YIDS AP+DGD+    K+PDFVE M+Y P+E V+M G YASKEEAKK
Sbjct: 241 IPVKGDLQALAQGYIDSFAPKDGDK---SKIPDFVEGMVYNPTEGVMMVGTYASKEEAKK 300

Query: 301 RGNVINSIGWWFKPWFYQHAATALKRGPFVEYIPTREYYHRHTRCLYWEGKLILPFADQW 360
           +GN IN++GWWFKPWFYQHA TALK+G FVEYIPTREYYHRHTRCLYWEGKLILPF DQ+
Sbjct: 301 KGNKINNVGWWFKPWFYQHAQTALKKGQFVEYIPTREYYHRHTRCLYWEGKLILPFGDQF 360

Query: 361 WFRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIIQDMLVPLYKVPDALEWAHRELEVYP 420
           WFR+LLGW+MPPKVSLLKATQGEAIRNYYH+MH+IQDMLVPLYKV DALEW HRE+EVYP
Sbjct: 361 WFRYLLGWLMPPKVSLLKATQGEAIRNYYHDMHVIQDMLVPLYKVGDALEWVHREMEVYP 420

Query: 421 IWLCPHRLFKLPVKTMIYPEPGFELHRRQGDTPYAQMYTDIGIYYAPGPVLRGEVFDGAG 480
           IWLCPH+LFK P+K  IYPEPGFE   RQGDT  AQMYTD+G+YYAPG VLRGE FDG+ 
Sbjct: 421 IWLCPHKLFKQPIKGQIYPEPGFEYENRQGDTEDAQMYTDVGVYYAPGCVLRGEEFDGSE 480

Query: 481 AVRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYNQCRKKYGAVGTFMTVYYKSKK 540
           AVRRMEKWL+ENHGFQ  YAVSEL+EK FWRMF+ ELY +CRKKY A+GTFM+VYYKSKK
Sbjct: 481 AVRRMEKWLIENHGFQPQYAVSELDEKSFWRMFNGELYEECRKKYRAIGTFMSVYYKSKK 540

Query: 541 GRKTEKEVKEAEQAHLETTYAELD 564
           GRKTEKEV+EAEQAHLET YAE D
Sbjct: 541 GRKTEKEVREAEQAHLETAYAEAD 561

BLAST of Sgr029579 vs. TAIR 10
Match: AT3G19820.3 (cell elongation protein / DWARF1 / DIMINUTO (DIM) )

HSP 1 Score: 939.9 bits (2428), Expect = 9.6e-274
Identity = 442/564 (78.37%), Postives = 500/564 (88.65%), Query Frame = 0

Query: 1   MSDLATPL-RPKRKKVWVDYFVQFRWILVVFVVLPVSFSLYFFTYLGDVRSAWKSYKQRQ 60
           MSDL TPL RPKRKK WVDYFV+FRWI+V+F+VLP S + YF  YLGD+ S  KS+++RQ
Sbjct: 1   MSDLQTPLVRPKRKKTWVDYFVKFRWIIVIFIVLPFSATFYFLIYLGDMWSESKSFEKRQ 60

Query: 61  KEHEENVKKIAKRLKERNPAKDGLVCTARKPWIAVGMRNVDYKRARHFGVDLSAFRNILE 120
           KEH+ENVKK+ KRLK R+ +KDGLVCTARKPWIAVGMRNVDYKRARHF VDL  FRNILE
Sbjct: 61  KEHDENVKKVIKRLKGRDASKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLGEFRNILE 120

Query: 121 IDKDRMIAKVEPLVNMGQISRATLPLNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180
           I+K++M A+VEPLVNMGQISRAT+P+NLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF
Sbjct: 121 INKEKMTARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180

Query: 181 SDTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTY 240
           +DTV AYEIVLA G +VRAT+DNEYSDL+YAIPWSQGT+GLLVAAEI+LI +KEYM++TY
Sbjct: 181 ADTVEAYEIVLAGGELVRATRDNEYSDLYYAIPWSQGTLGLLVAAEIRLIKVKEYMRLTY 240

Query: 241 TPFIGTLKEIGQAYIDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKK 300
            P  G L+ + Q YIDS AP+DGD+    K+PDFVE M+Y P+E V+M G YASKEEAKK
Sbjct: 241 IPVKGDLQALAQGYIDSFAPKDGDK---SKIPDFVEGMVYNPTEGVMMVGTYASKEEAKK 300

Query: 301 RGNVINSIGWWFKPWFYQHAATALKRGPFVEYIPTREYYHRHTRCLYWEGKLILPFADQW 360
           +GN IN++GWWFKPWFYQHA TALK+G FVEYIPTREYYHRHTRCLYWEGKLILPF DQ+
Sbjct: 301 KGNKINNVGWWFKPWFYQHAQTALKKGQFVEYIPTREYYHRHTRCLYWEGKLILPFGDQF 360

Query: 361 WFRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIIQDMLVPLYKVPDALEWAHRELEVYP 420
           WFR+LLGW+MPPKVSLLKATQGEAIRNYYH+MH+IQDMLVPLYKV DALEW HRE+EVYP
Sbjct: 361 WFRYLLGWLMPPKVSLLKATQGEAIRNYYHDMHVIQDMLVPLYKVGDALEWVHREMEVYP 420

Query: 421 IWLCPHRLFKLPVKTMIYPEPGFELHRRQGDTPYAQMYTDIGIYYAPGPVLRGEVFDGAG 480
           IWLCPH+LFK P+K  IYPEPGFE   RQGDT  AQMYTD+G+YYAPG VLRGE FDG+ 
Sbjct: 421 IWLCPHKLFKQPIKGQIYPEPGFEYENRQGDTEDAQMYTDVGVYYAPGCVLRGEEFDGSE 480

Query: 481 AVRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYNQCRKKYGAVGTFMTVYYKSKK 540
           AVRRMEKWL+ENHGFQ  YAVSEL+EK FWRMF+ ELY +CRKKY A+GTFM+VYYKSKK
Sbjct: 481 AVRRMEKWLIENHGFQPQYAVSELDEKSFWRMFNGELYEECRKKYRAIGTFMSVYYKSKK 540

Query: 541 GRKTEKEVKEAEQAHLETTYAELD 564
           GRKTEKEV+EAEQAHLET YAE D
Sbjct: 541 GRKTEKEVREAEQAHLETAYAEAD 561

BLAST of Sgr029579 vs. TAIR 10
Match: AT5G56970.1 (cytokinin oxidase 3 )

HSP 1 Score: 51.6 bits (122), Expect = 2.4e-06
Identity = 42/140 (30.00%), Postives = 64/140 (45.71%), Query Frame = 0

Query: 89  KPWIAVGMRNVDYKRARHFGVDLSAFRNILEIDKDRMIAKVEPLVNMGQISRATLPLNLS 148
           K  + V MR++     R  G+ +S  R  L +D D     +E L         TL L L+
Sbjct: 114 KDGVVVNMRSM---VNRDRGIKVS--RTCLYVDVDAAWLWIEVL-------NKTLELGLT 173

Query: 149 LAVVAELDDLTVGGLINGYGIEGSSHIYGLFSDTVVAYEIVLADGRVVRATKDNEYSDLF 208
                +   LTVGG ++  GI G +  YG     V+  +++   G +   +KD   SDLF
Sbjct: 174 PVSWTDYLYLTVGGTLSNGGISGQTFRYGPQITNVLEMDVITGKGEIATCSKDMN-SDLF 233

Query: 209 YAIPWSQGTIGLLVAAEIKL 229
           +A+    G  G++  A IKL
Sbjct: 234 FAVLGGLGQFGIITRARIKL 240

BLAST of Sgr029579 vs. TAIR 10
Match: AT2G41510.1 (cytokinin oxidase/dehydrogenase 1 )

HSP 1 Score: 50.1 bits (118), Expect = 7.0e-06
Identity = 42/144 (29.17%), Postives = 67/144 (46.53%), Query Frame = 0

Query: 138 ISRATLPLNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFSDTVVAYEIVLADGRVVR 197
           I R TL   LS     +   LTVGG ++  GI G +  +G   + V   EIV   G VV 
Sbjct: 170 ILRETLKYGLSPKSWTDYLHLTVGGTLSNAGISGQAFKHGPQINNVYQLEIVTGKGEVVT 229

Query: 198 ATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIP---IKEYMKVTYTPFIGTLKEIGQAYI 257
            + +   S+LF+++    G  G++  A I L P   + ++++V Y+ F    ++  Q Y+
Sbjct: 230 CS-EKRNSELFFSVLGGLGQFGIITRARISLEPAPHMVKWIRVLYSDFSAFSRD--QEYL 289

Query: 258 DSLAPRDGDQDNPEKVPDFVETMI 279
            S           EK  D+VE  +
Sbjct: 290 IS----------KEKTFDYVEGFV 300

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7014278.10.0e+0093.27Delta(24)-sterol reductase [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023548025.10.0e+0093.45delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548027.1 delta(24... [more]
XP_038898378.10.0e+0093.63delta(24)-sterol reductase [Benincasa hispida] >XP_038898379.1 delta(24)-sterol ... [more]
XP_022953594.10.0e+0093.10delta(24)-sterol reductase [Cucurbita moschata] >XP_022953595.1 delta(24)-sterol... [more]
XP_022991438.10.0e+0093.10delta(24)-sterol reductase [Cucurbita maxima] >XP_022991439.1 delta(24)-sterol r... [more]
Match NameE-valueIdentityDescription
P934721.1e-29082.30Delta(24)-sterol reductase OS=Pisum sativum OX=3888 GN=DIM PE=1 SV=1[more]
Q390851.4e-27278.37Delta(24)-sterol reductase OS=Arabidopsis thaliana OX=3702 GN=DIM PE=1 SV=2[more]
Q153922.9e-10541.90Delta(24)-sterol reductase OS=Homo sapiens OX=9606 GN=DHCR24 PE=1 SV=2[more]
Q60HC53.7e-10541.90Delta(24)-sterol reductase OS=Macaca fascicularis OX=9541 GN=DHCR24 PE=2 SV=2[more]
Q8VCH61.1e-10441.90Delta(24)-sterol reductase OS=Mus musculus OX=10090 GN=Dhcr24 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JUW60.0e+0093.10Delta(24)-sterol reductase OS=Cucurbita maxima OX=3661 GN=LOC111488060 PE=4 SV=1[more]
A0A6J1GQ500.0e+0093.10Delta(24)-sterol reductase OS=Cucurbita moschata OX=3662 GN=LOC111456086 PE=4 SV... [more]
A0A6J1H6V40.0e+0092.04Delta(24)-sterol reductase OS=Cucurbita moschata OX=3662 GN=LOC111460987 PE=4 SV... [more]
A0A6J1KZQ60.0e+0091.86Delta(24)-sterol reductase OS=Cucurbita maxima OX=3661 GN=LOC111497787 PE=4 SV=1[more]
A0A6J1DEM50.0e+0091.55Delta(24)-sterol reductase OS=Momordica charantia OX=3673 GN=LOC111019723 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT3G19820.19.6e-27478.37cell elongation protein / DWARF1 / DIMINUTO (DIM) [more]
AT3G19820.29.6e-27478.37cell elongation protein / DWARF1 / DIMINUTO (DIM) [more]
AT3G19820.39.6e-27478.37cell elongation protein / DWARF1 / DIMINUTO (DIM) [more]
AT5G56970.12.4e-0630.00cytokinin oxidase 3 [more]
AT2G41510.17.0e-0629.17cytokinin oxidase/dehydrogenase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 56..76
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 545..566
NoneNo IPR availablePANTHERPTHR10801:SF16S-LOCUS GLYCOPROTEIN DOMAIN, BULB-TYPE LECTIN DOMAIN, FAD-BINDING, TYPE 2-RELATEDcoord: 1..564
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 110..231
e-value: 2.1E-26
score: 94.0
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 107..198
e-value: 1.7E-14
score: 53.7
IPR040165Delta(24)-sterol reductasePANTHERPTHR1080124-DEHYDROCHOLESTEROL REDUCTASEcoord: 1..564
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 45..231
score: 13.349322
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 106..231

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr029579.1Sgr029579.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008202 steroid metabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0050614 delta24-sterol reductase activity
molecular_function GO:0071949 FAD binding
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016491 oxidoreductase activity