HG10016545 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10016545
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionAlpha/beta hydrolase-1
LocationChr03: 5816021 .. 5817475 (+)
RNA-Seq ExpressionHG10016545
SyntenyHG10016545
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGGCCAGTGTTTCAGATACGGATCTATCATGGAGGCGTGGTACCGTTCCTGTTTCTCCAGAGTGGGTCTTAAATCCGCCACCACCGATCTCGGCAACGGCACCGTCATGCACTGCTGGATCCCCAAAACCCCCAATCAAACCAAGCCCAATTTGGTTCTCATCCATGGCATGGGAGCAAACGCAGTGTGGCAATGGAACAAATTCGTCCGCCCACTCATCCCCCATTTCAACATCTATGTCCCCGATTTGGTCTTCTTTGGGGAATCCTACACAACCCTTTCCGATCGCTCCGAGGCATTTCAGGCTCGCTCCGTCATGGCGGTTTTGGATGCCCATGGTGTACGGAATACAAACGCCGTCGGGATAAGCTATGGAGGGTTTGTGGTTTATAGCATGGCGGCACAGTTTCCTGATAGAATGGAGAAATTGGTGCTTTGTTGTACTGGGGTTTGCTTGGAAGATAAAGATATGGAAGATGGGATGTTCCAAGTGAAGTCTGTGGATGAAGCAGTGAGCGTCTTGCTTCCTCAATCGCCTGAGAAATTGAAGGAAATGATACAAATTGCGTTTTTTAAGCCTATTAGAATTGGACCCACTTGCTTGGTTAACGACCTTATCGATGTAAGTAATCATTCACTTCAGTTTGTTTCGTTTATTCATCGTTTTTTGCCTTGACGTGTTGAACGATTGTGGCTAATTTACTTGATCTTTTCTGATTTCTTGACTAGCATCTGCATCTTGTTAATGGGTTCCTATTAAATTTGTCTTTCTGAGTTGTGACAAACATTGATCTGAAACGAGCTTACTCTACGGTCAGAAAATAGCTTAGTTTTAAAATGTCATTTGATTTTACTGTAGGAATTGTGTACCGAGTATCGTGAACAAAAGAAGGAGCTGATTCAAGCATTACACAAGGAAAGGAAATTGTCAAATCTTCCCAAGATTTCTCAGGTAATTCCTCTCATTATAAAAGATTTCAGAGTTTGGATTTCCTTTTAAAATTTGTTCCACTGTTCTGATGAGATGATTAACATTTGACACAGCCTACACTTATAATGTGGGGAGAGAAAGACTTGGTATTCCCTATGGAATTGGCACACAGATTAAAAAGGTGAATCTAATGAACTTAATCCGCTTCCACACTTGTTTACTTATAGAAGATTTTTACAAAAGTAGTTTTGTTACAATTATCTCTCTTTATACTGGAAATTTAGTTGGTTTCCCTTTCTACTTTTGTGGGTTTTGGTTAATCTCTTCAATTTTGGTATTGATTTGTACTGCAGGCACATAGGTGATAGTGCAGAACTAGTGGTTATAAAGAAAGCAGGACATGCTTTAAATATAGAGAAGCCAAAGGAGATGAACAAGCTTATTCAGTGTTTCCTCGTTGATGCTGTCCCTTCAACGAAGGCCAAAATACACCACCAAAATGACCTTAAATCTGAGTGA

mRNA sequence

ATGGCTGGCCAGTGTTTCAGATACGGATCTATCATGGAGGCGTGGTACCGTTCCTGTTTCTCCAGAGTGGGTCTTAAATCCGCCACCACCGATCTCGGCAACGGCACCGTCATGCACTGCTGGATCCCCAAAACCCCCAATCAAACCAAGCCCAATTTGGTTCTCATCCATGGCATGGGAGCAAACGCAGTGTGGCAATGGAACAAATTCGTCCGCCCACTCATCCCCCATTTCAACATCTATGTCCCCGATTTGGTCTTCTTTGGGGAATCCTACACAACCCTTTCCGATCGCTCCGAGGCATTTCAGGCTCGCTCCGTCATGGCGGTTTTGGATGCCCATGGTGTACGGAATACAAACGCCGTCGGGATAAGCTATGGAGGGTTTGTGGTTTATAGCATGGCGGCACAGTTTCCTGATAGAATGGAGAAATTGGTGCTTTGTTGTACTGGGGTTTGCTTGGAAGATAAAGATATGGAAGATGGGATGTTCCAAGTGAAGTCTGTGGATGAAGCAGTGAGCGTCTTGCTTCCTCAATCGCCTGAGAAATTGAAGGAAATGATACAAATTGCGTTTTTTAAGCCTATTAGAATTGGACCCACTTGCTTGGTTAACGACCTTATCGATGAATTGTGTACCGAGTATCGTGAACAAAAGAAGGAGCTGATTCAAGCATTACACAAGGAAAGGAAATTGTCAAATCTTCCCAAGATTTCTCAGCCTACACTTATAATGTGGGGAGAGAAAGACTTGGTATTCCCTATGGAATTGGCACACAGATTAAAAAGGCACATAGGTGATAGTGCAGAACTAGTGGTTATAAAGAAAGCAGGACATGCTTTAAATATAGAGAAGCCAAAGGAGATGAACAAGCTTATTCAGTGTTTCCTCGTTGATGCTGTCCCTTCAACGAAGGCCAAAATACACCACCAAAATGACCTTAAATCTGAGTGA

Coding sequence (CDS)

ATGGCTGGCCAGTGTTTCAGATACGGATCTATCATGGAGGCGTGGTACCGTTCCTGTTTCTCCAGAGTGGGTCTTAAATCCGCCACCACCGATCTCGGCAACGGCACCGTCATGCACTGCTGGATCCCCAAAACCCCCAATCAAACCAAGCCCAATTTGGTTCTCATCCATGGCATGGGAGCAAACGCAGTGTGGCAATGGAACAAATTCGTCCGCCCACTCATCCCCCATTTCAACATCTATGTCCCCGATTTGGTCTTCTTTGGGGAATCCTACACAACCCTTTCCGATCGCTCCGAGGCATTTCAGGCTCGCTCCGTCATGGCGGTTTTGGATGCCCATGGTGTACGGAATACAAACGCCGTCGGGATAAGCTATGGAGGGTTTGTGGTTTATAGCATGGCGGCACAGTTTCCTGATAGAATGGAGAAATTGGTGCTTTGTTGTACTGGGGTTTGCTTGGAAGATAAAGATATGGAAGATGGGATGTTCCAAGTGAAGTCTGTGGATGAAGCAGTGAGCGTCTTGCTTCCTCAATCGCCTGAGAAATTGAAGGAAATGATACAAATTGCGTTTTTTAAGCCTATTAGAATTGGACCCACTTGCTTGGTTAACGACCTTATCGATGAATTGTGTACCGAGTATCGTGAACAAAAGAAGGAGCTGATTCAAGCATTACACAAGGAAAGGAAATTGTCAAATCTTCCCAAGATTTCTCAGCCTACACTTATAATGTGGGGAGAGAAAGACTTGGTATTCCCTATGGAATTGGCACACAGATTAAAAAGGCACATAGGTGATAGTGCAGAACTAGTGGTTATAAAGAAAGCAGGACATGCTTTAAATATAGAGAAGCCAAAGGAGATGAACAAGCTTATTCAGTGTTTCCTCGTTGATGCTGTCCCTTCAACGAAGGCCAAAATACACCACCAAAATGACCTTAAATCTGAGTGA

Protein sequence

MAGQCFRYGSIMEAWYRSCFSRVGLKSATTDLGNGTVMHCWIPKTPNQTKPNLVLIHGMGANAVWQWNKFVRPLIPHFNIYVPDLVFFGESYTTLSDRSEAFQARSVMAVLDAHGVRNTNAVGISYGGFVVYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVDEAVSVLLPQSPEKLKEMIQIAFFKPIRIGPTCLVNDLIDELCTEYREQKKELIQALHKERKLSNLPKISQPTLIMWGEKDLVFPMELAHRLKRHIGDSAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAVPSTKAKIHHQNDLKSE
Homology
BLAST of HG10016545 vs. NCBI nr
Match: XP_038882658.1 (2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase-like [Benincasa hispida])

HSP 1 Score: 627.9 bits (1618), Expect = 4.9e-176
Identity = 300/317 (94.64%), Postives = 311/317 (98.11%), Query Frame = 0

Query: 1   MAGQCFRYGSIMEAWYRSCFSRVGLKSATTDLGNGTVMHCWIPKTPNQTKPNLVLIHGMG 60
           MAGQCFRYGSIM++WYRSCFSRVGLKSATTDLGNGTVMHCWIPK P QTKPNLVLIHGMG
Sbjct: 1   MAGQCFRYGSIMDSWYRSCFSRVGLKSATTDLGNGTVMHCWIPKNPKQTKPNLVLIHGMG 60

Query: 61  ANAVWQWNKFVRPLIPHFNIYVPDLVFFGESYTTLSDRSEAFQARSVMAVLDAHGVRNTN 120
           ANA+WQWN+F RPLI HFNIYVPDLVFFGESYTTLSDRSEAFQARSVMAVLDAHGVR TN
Sbjct: 61  ANAMWQWNQFARPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARSVMAVLDAHGVRTTN 120

Query: 121 AVGISYGGFVVYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVDEAVSVLLPQS 180
           AVGISYGGFV YSMAAQFPDRMEKLVLCCTGVCLED+DMEDGMFQVKSVDEAVSVLLPQS
Sbjct: 121 AVGISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDQDMEDGMFQVKSVDEAVSVLLPQS 180

Query: 181 PEKLKEMIQIAFFKPIRIGPTCLVNDLIDELCTEYREQKKELIQALHKERKLSNLPKISQ 240
           PEKLKEMIQIAFFKPIRIGP+CLVNDLIDELCTEYREQKKELIQALHKERKLSNLPKI+Q
Sbjct: 181 PEKLKEMIQIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKLSNLPKITQ 240

Query: 241 PTLIMWGEKDLVFPMELAHRLKRHIGDSAELVVIKKAGHALNIEKPKEMNKLIQCFLVDA 300
           PTLI+WGEKDLVFPMELAHRLKRHIGDSAELVVIKKAGHALNIEKPKEMNKLIQCFLVD+
Sbjct: 241 PTLIIWGEKDLVFPMELAHRLKRHIGDSAELVVIKKAGHALNIEKPKEMNKLIQCFLVDS 300

Query: 301 VPSTKAKIHHQNDLKSE 318
           +PSTKAKIHHQ+DLKSE
Sbjct: 301 IPSTKAKIHHQSDLKSE 317

BLAST of HG10016545 vs. NCBI nr
Match: XP_008440587.1 (PREDICTED: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase-like [Cucumis melo] >KAA0036310.1 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase-like [Cucumis melo var. makuwa] >TYK12704.1 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase-like [Cucumis melo var. makuwa])

HSP 1 Score: 622.1 bits (1603), Expect = 2.7e-174
Identity = 298/317 (94.01%), Postives = 307/317 (96.85%), Query Frame = 0

Query: 1   MAGQCFRYGSIMEAWYRSCFSRVGLKSATTDLGNGTVMHCWIPKTPNQTKPNLVLIHGMG 60
           M GQCF YGSIMEAWYRSCFSRVGLKSATTDLGNGTVMHCWIPKTP QTKPNLVLIHGMG
Sbjct: 1   MGGQCFSYGSIMEAWYRSCFSRVGLKSATTDLGNGTVMHCWIPKTPKQTKPNLVLIHGMG 60

Query: 61  ANAVWQWNKFVRPLIPHFNIYVPDLVFFGESYTTLSDRSEAFQARSVMAVLDAHGVRNTN 120
           ANA+WQWN+FVRPLI HFNIYVPDLVFFGESYTTLSDRSEAFQAR VM VLDAHGVR TN
Sbjct: 61  ANAMWQWNQFVRPLITHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTN 120

Query: 121 AVGISYGGFVVYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVDEAVSVLLPQS 180
           AVGISYGGFV YSMAAQFPDRMEKLVLCCTGVCLED+DMEDGMFQVKSV+EAVSVLLPQS
Sbjct: 121 AVGISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDQDMEDGMFQVKSVEEAVSVLLPQS 180

Query: 181 PEKLKEMIQIAFFKPIRIGPTCLVNDLIDELCTEYREQKKELIQALHKERKLSNLPKISQ 240
           PEKLKEMI+IAFFKPIRIGP+C+VNDLIDELCTEYREQKKELIQALHKERKLSNLPKI+ 
Sbjct: 181 PEKLKEMIKIAFFKPIRIGPSCVVNDLIDELCTEYREQKKELIQALHKERKLSNLPKITN 240

Query: 241 PTLIMWGEKDLVFPMELAHRLKRHIGDSAELVVIKKAGHALNIEKPKEMNKLIQCFLVDA 300
           PTLIMWGEKDLVFPMELAHRLKRHIG+ AELVVIKKAGHALNIEKPKEMNKLIQCFLVDA
Sbjct: 241 PTLIMWGEKDLVFPMELAHRLKRHIGEGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDA 300

Query: 301 VPSTKAKIHHQNDLKSE 318
           VPSTKAKIHHQNDLKSE
Sbjct: 301 VPSTKAKIHHQNDLKSE 317

BLAST of HG10016545 vs. NCBI nr
Match: XP_004143463.1 (uncharacterized protein LOC101208157 [Cucumis sativus] >KGN48741.1 hypothetical protein Csa_003107 [Cucumis sativus])

HSP 1 Score: 616.3 bits (1588), Expect = 1.5e-172
Identity = 293/317 (92.43%), Postives = 306/317 (96.53%), Query Frame = 0

Query: 1   MAGQCFRYGSIMEAWYRSCFSRVGLKSATTDLGNGTVMHCWIPKTPNQTKPNLVLIHGMG 60
           M GQCF YGSIM+AWYRSCFSRVGL SATTDLGNGTVMHCWIPKTP +TKPNLVLIHGMG
Sbjct: 1   MGGQCFSYGSIMDAWYRSCFSRVGLTSATTDLGNGTVMHCWIPKTPKETKPNLVLIHGMG 60

Query: 61  ANAVWQWNKFVRPLIPHFNIYVPDLVFFGESYTTLSDRSEAFQARSVMAVLDAHGVRNTN 120
           ANA+WQWN+FVRPL+ HFNIYVPDLVFFGESYTTLSDRSEAFQAR VM VLDAHGVR TN
Sbjct: 61  ANAMWQWNQFVRPLVSHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTN 120

Query: 121 AVGISYGGFVVYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVDEAVSVLLPQS 180
           AVG+SYGGFV YSMAAQFPDR+EKLVLCCTGVCLED+DMEDGMFQVKSV+EAVSVLLPQS
Sbjct: 121 AVGVSYGGFVAYSMAAQFPDRVEKLVLCCTGVCLEDQDMEDGMFQVKSVEEAVSVLLPQS 180

Query: 181 PEKLKEMIQIAFFKPIRIGPTCLVNDLIDELCTEYREQKKELIQALHKERKLSNLPKISQ 240
           PEKLKEMI+IAFFKPIRIGP+CLVNDLIDELCTEYREQKKELIQALHKERKLSNLPKI+ 
Sbjct: 181 PEKLKEMIKIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKLSNLPKITN 240

Query: 241 PTLIMWGEKDLVFPMELAHRLKRHIGDSAELVVIKKAGHALNIEKPKEMNKLIQCFLVDA 300
           PTLIMWGEKDLVFPMELAHRLKRHIG+ AELVVIKKAGHALNIEKPKEMNKLIQCFLVDA
Sbjct: 241 PTLIMWGEKDLVFPMELAHRLKRHIGEGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDA 300

Query: 301 VPSTKAKIHHQNDLKSE 318
           VPSTKAKIHHQNDLKSE
Sbjct: 301 VPSTKAKIHHQNDLKSE 317

BLAST of HG10016545 vs. NCBI nr
Match: XP_022950912.1 (uncharacterized protein LOC111453865 [Cucurbita moschata] >XP_022978531.1 uncharacterized protein LOC111478485 [Cucurbita maxima] >KAG6604037.1 Monoacylglycerol lipase ABHD6, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034202.1 Monoacylglycerol lipase ABHD6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 604.7 bits (1558), Expect = 4.4e-169
Identity = 291/314 (92.68%), Postives = 301/314 (95.86%), Query Frame = 0

Query: 4   QCFRYGSIMEAWYRSCFSRVGLKSATTDLGNGTVMHCWIPKTPNQTKPNLVLIHGMGANA 63
           QCFRYGSIMEAWYRSCFSRVGL+SATTDLGNGTVMHCWIPKT  +TKPNLVLIHGMGANA
Sbjct: 3   QCFRYGSIMEAWYRSCFSRVGLRSATTDLGNGTVMHCWIPKTRRETKPNLVLIHGMGANA 62

Query: 64  VWQWNKFVRPLIPHFNIYVPDLVFFGESYTTLSDRSEAFQARSVMAVLDAHGVRNTNAVG 123
           VWQWNKFVRPLI HFNIYVPDLVFFGESYTTLSDRSEAFQAR VM VLDAHGVR TNAVG
Sbjct: 63  VWQWNKFVRPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNAVG 122

Query: 124 ISYGGFVVYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVDEAVSVLLPQSPEK 183
           ISYGGFV YSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSV+EAVSVLLPQ+PEK
Sbjct: 123 ISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVEEAVSVLLPQTPEK 182

Query: 184 LKEMIQIAFFKPIRIGPTCLVNDLIDELCTEYREQKKELIQALHKERKLSNLPKISQPTL 243
           LKEMI+IAFFKPIRIGP+CLVNDLIDELCTEYREQKKELIQALHKERK S+LPKI+QPTL
Sbjct: 183 LKEMIRIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKFSDLPKITQPTL 242

Query: 244 IMWGEKDLVFPMELAHRLKRHIGDSAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAVPS 303
           IMWGEKDLVFPMELAHRLK HIGD AELVVIKKAGHALNIEKPKEMNKLIQCFLVDA  S
Sbjct: 243 IMWGEKDLVFPMELAHRLKSHIGDGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAHQS 302

Query: 304 TKAKIHHQNDLKSE 318
           ++AKIHHQND KSE
Sbjct: 303 SQAKIHHQNDPKSE 316

BLAST of HG10016545 vs. NCBI nr
Match: XP_023543119.1 (uncharacterized protein LOC111803081 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 602.8 bits (1553), Expect = 1.7e-168
Identity = 290/314 (92.36%), Postives = 300/314 (95.54%), Query Frame = 0

Query: 4   QCFRYGSIMEAWYRSCFSRVGLKSATTDLGNGTVMHCWIPKTPNQTKPNLVLIHGMGANA 63
           QCFRYGSIMEAWYRSCFSRVGL+SATTDLGNGTVMHCWIPKT  +TKPNLVLIHGMGANA
Sbjct: 3   QCFRYGSIMEAWYRSCFSRVGLRSATTDLGNGTVMHCWIPKTRRETKPNLVLIHGMGANA 62

Query: 64  VWQWNKFVRPLIPHFNIYVPDLVFFGESYTTLSDRSEAFQARSVMAVLDAHGVRNTNAVG 123
           VWQWNKFVRPLI HFNIYVPDLVFFGESYTTLSDRSEAFQAR VM VLDAHGVR  NAVG
Sbjct: 63  VWQWNKFVRPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTANAVG 122

Query: 124 ISYGGFVVYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVDEAVSVLLPQSPEK 183
           ISYGGFV YSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSV+EAVSVLLPQ+PEK
Sbjct: 123 ISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVEEAVSVLLPQTPEK 182

Query: 184 LKEMIQIAFFKPIRIGPTCLVNDLIDELCTEYREQKKELIQALHKERKLSNLPKISQPTL 243
           LKEMI+IAFFKPIRIGP+CLVNDLIDELCTEYREQKKELIQALHKERK S+LPKI+QPTL
Sbjct: 183 LKEMIRIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKFSDLPKITQPTL 242

Query: 244 IMWGEKDLVFPMELAHRLKRHIGDSAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAVPS 303
           IMWGEKDLVFPMELAHRLK HIGD AELVVIKKAGHALNIEKPKEMNKLIQCFLVDA  S
Sbjct: 243 IMWGEKDLVFPMELAHRLKSHIGDGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAHQS 302

Query: 304 TKAKIHHQNDLKSE 318
           ++AKIHHQND KSE
Sbjct: 303 SQAKIHHQNDPKSE 316

BLAST of HG10016545 vs. ExPASy Swiss-Prot
Match: Q52532 (2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Pseudomonas sp. OX=306 GN=mhpC PE=3 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 1.4e-13
Identity = 68/260 (26.15%), Postives = 116/260 (44.62%), Query Frame = 0

Query: 53  LVLIHGMGANAVWQWNKFVRPLIPH----FNIYVPDLVFFGESYTTLSDRSEAFQARSVM 112
           +V+ HG G  A   W+ F R +       + + + D   F +SY  ++   +   A++  
Sbjct: 36  VVMFHGSGPGAS-GWSNFHRNVDAFVDAGYRVLLIDSPGFNKSYPIVTKSRDGAYAQAAK 95

Query: 113 AVLDAHGVRNTNAVGISYGGFVVYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKS 172
            V+D  G++  + +G S GG     MA  +P+ + KLV+   G          G      
Sbjct: 96  GVMDKLGIKRAHMIGNSMGGATAMRMAVDYPEMVGKLVMMGGG-------SVGGSTTTPM 155

Query: 173 VDEAVSVL--LPQSP--EKLKEMIQIAFFKPIRIGPTCLVNDLID---ELCTEYREQKKE 232
             E + +L  L ++P  E L++M+ I  +      P+ L  +LI+   E      E    
Sbjct: 156 PTEGLKLLQGLYRNPSMENLRKMLDIFVY-----APSTLTEELINGRFENMMRRPEHLTN 215

Query: 233 LIQALHKERKLSN----LPKISQPTLIMWGEKDLVFPMELAHRLKRHIGDSAELVVIKKA 292
            +++L      +N    LP ++ PT+I+WG  D   P++L  R+   + D AEL V  K 
Sbjct: 216 FVESLKASGGRANYAHLLPTLTMPTMIIWGRDDRFVPLDLGLRMLWGMPD-AELHVFSKC 275

Query: 293 GHALNIEKPKEMNKLIQCFL 298
           GH    E   + N+L+  FL
Sbjct: 276 GHWAQWEHADKFNQLVLNFL 281

BLAST of HG10016545 vs. ExPASy Swiss-Prot
Match: Q52036 (2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase OS=Pseudomonas putida OX=303 GN=bphD PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 4.2e-13
Identity = 68/264 (25.76%), Postives = 112/264 (42.42%), Query Frame = 0

Query: 53  LVLIHGMGANAVWQWNKFVRPLIPH----FNIYVPDLVFFGESYTTLSDRSEAF-QARSV 112
           ++++HG G  A   W+ + R + P     + + + D   F +S   + D       AR+V
Sbjct: 36  VIMLHGGGPGA-GGWSNYYRNIGPFVEAGYRVILKDSPGFNKSDAVVMDEQRGLVNARAV 95

Query: 113 MAVLDAHGVRNTNAVGISYGGFVVYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMF--- 172
             ++DA G+   + VG S GG    + A ++PDR+ KL+L   G       +   MF   
Sbjct: 96  KGLMDALGIDRAHLVGNSMGGATALNFAIEYPDRIGKLILMGPG------GLGPSMFAPM 155

Query: 173 QVKSVDEAVSVLLPQSPEKLKEMIQIAFFKPIRIGPTCLVNDLIDELCTEYREQKKELIQ 232
            ++ +     +    S E LK+MIQ+  +       + +  +L+       + Q + L  
Sbjct: 156 PLEGIKLLFKLYAEPSYENLKQMIQVFLY-----DQSLITEELLQGRWEAIQRQPEHLKN 215

Query: 233 ALHKERK--------LSNLPKISQPTLIMWGEKDLVFPMELAHRLKRHIGDSAELVVIKK 292
            L   +K         + L +I   T I WG  D   P++   +L  +I D A L V  K
Sbjct: 216 FLISAQKAPLSTWDVTARLGEIKAKTFITWGRDDRFVPLDHGLKLLWNI-DDARLHVFSK 275

Query: 293 AGHALNIEKPKEMNKLIQCFLVDA 301
            GH    E   E N+L   FL  A
Sbjct: 276 CGHWAQWEHADEFNRLAIDFLRQA 286

BLAST of HG10016545 vs. ExPASy Swiss-Prot
Match: P9WNH2 (Uncharacterized protein MT2788 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=MT2788 PE=3 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 7.2e-13
Identity = 67/274 (24.45%), Postives = 115/274 (41.97%), Query Frame = 0

Query: 51  PNLVLIHGMGANAVWQWNKFVRPLIPHFNIYVPDLVFFGESYTTLSDRSEAFQARSVMAV 110
           P ++LIHG+G N+   WN     L   F +  PDL+  G+S    +D S A  A  +  +
Sbjct: 38  PAILLIHGIGDNST-TWNGVHAKLAQRFTVIAPDLLGHGQSDKPRADYSVAAYANGMRDL 97

Query: 111 LDAHGVRNTNAVGISYGGFVVYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVD 170
           L    +     VG S GG V    A QFP  +++L+L   G   +D ++   +  +    
Sbjct: 98  LSVLDIERVTIVGHSLGGGVAMQFAYQFPQLVDRLILVSAGGVTKDVNIVFRLASLPMGS 157

Query: 171 EAVSVLLPQSPEKLKEMIQIAFFKPIRIGPTCLVNDL------IDEL------------- 230
           EA+++L  + P  L  +          IG T L +DL      +D+L             
Sbjct: 158 EAMALL--RLPLVLPAVQIAGRIVGKAIGTTSLGHDLPNVLRILDDLPEPTASAAFGRTL 217

Query: 231 --CTEYREQKKELIQALHKERKLSNLPKISQPTLIMWGEKDLVFPMELAHRLKRHIGDSA 290
               ++R Q   ++   +    +        P  I+WG KD+V P+  AH     +  S 
Sbjct: 218 RAVVDWRGQMVTMLDRCYLTEAI--------PVQIIWGTKDVVLPVRHAHMAHAAMPGS- 277

Query: 291 ELVVIKKAGHALNIEKPKEMNKLIQCFLVDAVPS 304
           +L + + +GH    + P     +++ F+    P+
Sbjct: 278 QLEIFEGSGHFPFHDDPARFIDIVERFMDTTEPA 299

BLAST of HG10016545 vs. ExPASy Swiss-Prot
Match: P9WNH3 (Uncharacterized protein Rv2715 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=Rv2715 PE=1 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 7.2e-13
Identity = 67/274 (24.45%), Postives = 115/274 (41.97%), Query Frame = 0

Query: 51  PNLVLIHGMGANAVWQWNKFVRPLIPHFNIYVPDLVFFGESYTTLSDRSEAFQARSVMAV 110
           P ++LIHG+G N+   WN     L   F +  PDL+  G+S    +D S A  A  +  +
Sbjct: 38  PAILLIHGIGDNST-TWNGVHAKLAQRFTVIAPDLLGHGQSDKPRADYSVAAYANGMRDL 97

Query: 111 LDAHGVRNTNAVGISYGGFVVYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVD 170
           L    +     VG S GG V    A QFP  +++L+L   G   +D ++   +  +    
Sbjct: 98  LSVLDIERVTIVGHSLGGGVAMQFAYQFPQLVDRLILVSAGGVTKDVNIVFRLASLPMGS 157

Query: 171 EAVSVLLPQSPEKLKEMIQIAFFKPIRIGPTCLVNDL------IDEL------------- 230
           EA+++L  + P  L  +          IG T L +DL      +D+L             
Sbjct: 158 EAMALL--RLPLVLPAVQIAGRIVGKAIGTTSLGHDLPNVLRILDDLPEPTASAAFGRTL 217

Query: 231 --CTEYREQKKELIQALHKERKLSNLPKISQPTLIMWGEKDLVFPMELAHRLKRHIGDSA 290
               ++R Q   ++   +    +        P  I+WG KD+V P+  AH     +  S 
Sbjct: 218 RAVVDWRGQMVTMLDRCYLTEAI--------PVQIIWGTKDVVLPVRHAHMAHAAMPGS- 277

Query: 291 ELVVIKKAGHALNIEKPKEMNKLIQCFLVDAVPS 304
           +L + + +GH    + P     +++ F+    P+
Sbjct: 278 QLEIFEGSGHFPFHDDPARFIDIVERFMDTTEPA 299

BLAST of HG10016545 vs. ExPASy Swiss-Prot
Match: P0A573 (Uncharacterized protein Mb2734 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=BQ2027_MB2734 PE=3 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 7.2e-13
Identity = 67/274 (24.45%), Postives = 115/274 (41.97%), Query Frame = 0

Query: 51  PNLVLIHGMGANAVWQWNKFVRPLIPHFNIYVPDLVFFGESYTTLSDRSEAFQARSVMAV 110
           P ++LIHG+G N+   WN     L   F +  PDL+  G+S    +D S A  A  +  +
Sbjct: 38  PAILLIHGIGDNST-TWNGVHAKLAQRFTVIAPDLLGHGQSDKPRADYSVAAYANGMRDL 97

Query: 111 LDAHGVRNTNAVGISYGGFVVYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVD 170
           L    +     VG S GG V    A QFP  +++L+L   G   +D ++   +  +    
Sbjct: 98  LSVLDIERVTIVGHSLGGGVAMQFAYQFPQLVDRLILVSAGGVTKDVNIVFRLASLPMGS 157

Query: 171 EAVSVLLPQSPEKLKEMIQIAFFKPIRIGPTCLVNDL------IDEL------------- 230
           EA+++L  + P  L  +          IG T L +DL      +D+L             
Sbjct: 158 EAMALL--RLPLVLPAVQIAGRIVGKAIGTTSLGHDLPNVLRILDDLPEPTASAAFGRTL 217

Query: 231 --CTEYREQKKELIQALHKERKLSNLPKISQPTLIMWGEKDLVFPMELAHRLKRHIGDSA 290
               ++R Q   ++   +    +        P  I+WG KD+V P+  AH     +  S 
Sbjct: 218 RAVVDWRGQMVTMLDRCYLTEAI--------PVQIIWGTKDVVLPVRHAHMAHAAMPGS- 277

Query: 291 ELVVIKKAGHALNIEKPKEMNKLIQCFLVDAVPS 304
           +L + + +GH    + P     +++ F+    P+
Sbjct: 278 QLEIFEGSGHFPFHDDPARFIDIVERFMDTTEPA 299

BLAST of HG10016545 vs. ExPASy TrEMBL
Match: A0A5A7SYZ3 (2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002920 PE=4 SV=1)

HSP 1 Score: 622.1 bits (1603), Expect = 1.3e-174
Identity = 298/317 (94.01%), Postives = 307/317 (96.85%), Query Frame = 0

Query: 1   MAGQCFRYGSIMEAWYRSCFSRVGLKSATTDLGNGTVMHCWIPKTPNQTKPNLVLIHGMG 60
           M GQCF YGSIMEAWYRSCFSRVGLKSATTDLGNGTVMHCWIPKTP QTKPNLVLIHGMG
Sbjct: 1   MGGQCFSYGSIMEAWYRSCFSRVGLKSATTDLGNGTVMHCWIPKTPKQTKPNLVLIHGMG 60

Query: 61  ANAVWQWNKFVRPLIPHFNIYVPDLVFFGESYTTLSDRSEAFQARSVMAVLDAHGVRNTN 120
           ANA+WQWN+FVRPLI HFNIYVPDLVFFGESYTTLSDRSEAFQAR VM VLDAHGVR TN
Sbjct: 61  ANAMWQWNQFVRPLITHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTN 120

Query: 121 AVGISYGGFVVYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVDEAVSVLLPQS 180
           AVGISYGGFV YSMAAQFPDRMEKLVLCCTGVCLED+DMEDGMFQVKSV+EAVSVLLPQS
Sbjct: 121 AVGISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDQDMEDGMFQVKSVEEAVSVLLPQS 180

Query: 181 PEKLKEMIQIAFFKPIRIGPTCLVNDLIDELCTEYREQKKELIQALHKERKLSNLPKISQ 240
           PEKLKEMI+IAFFKPIRIGP+C+VNDLIDELCTEYREQKKELIQALHKERKLSNLPKI+ 
Sbjct: 181 PEKLKEMIKIAFFKPIRIGPSCVVNDLIDELCTEYREQKKELIQALHKERKLSNLPKITN 240

Query: 241 PTLIMWGEKDLVFPMELAHRLKRHIGDSAELVVIKKAGHALNIEKPKEMNKLIQCFLVDA 300
           PTLIMWGEKDLVFPMELAHRLKRHIG+ AELVVIKKAGHALNIEKPKEMNKLIQCFLVDA
Sbjct: 241 PTLIMWGEKDLVFPMELAHRLKRHIGEGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDA 300

Query: 301 VPSTKAKIHHQNDLKSE 318
           VPSTKAKIHHQNDLKSE
Sbjct: 301 VPSTKAKIHHQNDLKSE 317

BLAST of HG10016545 vs. ExPASy TrEMBL
Match: A0A1S3B1G1 (2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase-like OS=Cucumis melo OX=3656 GN=LOC103484964 PE=4 SV=1)

HSP 1 Score: 622.1 bits (1603), Expect = 1.3e-174
Identity = 298/317 (94.01%), Postives = 307/317 (96.85%), Query Frame = 0

Query: 1   MAGQCFRYGSIMEAWYRSCFSRVGLKSATTDLGNGTVMHCWIPKTPNQTKPNLVLIHGMG 60
           M GQCF YGSIMEAWYRSCFSRVGLKSATTDLGNGTVMHCWIPKTP QTKPNLVLIHGMG
Sbjct: 1   MGGQCFSYGSIMEAWYRSCFSRVGLKSATTDLGNGTVMHCWIPKTPKQTKPNLVLIHGMG 60

Query: 61  ANAVWQWNKFVRPLIPHFNIYVPDLVFFGESYTTLSDRSEAFQARSVMAVLDAHGVRNTN 120
           ANA+WQWN+FVRPLI HFNIYVPDLVFFGESYTTLSDRSEAFQAR VM VLDAHGVR TN
Sbjct: 61  ANAMWQWNQFVRPLITHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTN 120

Query: 121 AVGISYGGFVVYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVDEAVSVLLPQS 180
           AVGISYGGFV YSMAAQFPDRMEKLVLCCTGVCLED+DMEDGMFQVKSV+EAVSVLLPQS
Sbjct: 121 AVGISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDQDMEDGMFQVKSVEEAVSVLLPQS 180

Query: 181 PEKLKEMIQIAFFKPIRIGPTCLVNDLIDELCTEYREQKKELIQALHKERKLSNLPKISQ 240
           PEKLKEMI+IAFFKPIRIGP+C+VNDLIDELCTEYREQKKELIQALHKERKLSNLPKI+ 
Sbjct: 181 PEKLKEMIKIAFFKPIRIGPSCVVNDLIDELCTEYREQKKELIQALHKERKLSNLPKITN 240

Query: 241 PTLIMWGEKDLVFPMELAHRLKRHIGDSAELVVIKKAGHALNIEKPKEMNKLIQCFLVDA 300
           PTLIMWGEKDLVFPMELAHRLKRHIG+ AELVVIKKAGHALNIEKPKEMNKLIQCFLVDA
Sbjct: 241 PTLIMWGEKDLVFPMELAHRLKRHIGEGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDA 300

Query: 301 VPSTKAKIHHQNDLKSE 318
           VPSTKAKIHHQNDLKSE
Sbjct: 301 VPSTKAKIHHQNDLKSE 317

BLAST of HG10016545 vs. ExPASy TrEMBL
Match: A0A0A0KLW0 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G499850 PE=4 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 7.1e-173
Identity = 293/317 (92.43%), Postives = 306/317 (96.53%), Query Frame = 0

Query: 1   MAGQCFRYGSIMEAWYRSCFSRVGLKSATTDLGNGTVMHCWIPKTPNQTKPNLVLIHGMG 60
           M GQCF YGSIM+AWYRSCFSRVGL SATTDLGNGTVMHCWIPKTP +TKPNLVLIHGMG
Sbjct: 1   MGGQCFSYGSIMDAWYRSCFSRVGLTSATTDLGNGTVMHCWIPKTPKETKPNLVLIHGMG 60

Query: 61  ANAVWQWNKFVRPLIPHFNIYVPDLVFFGESYTTLSDRSEAFQARSVMAVLDAHGVRNTN 120
           ANA+WQWN+FVRPL+ HFNIYVPDLVFFGESYTTLSDRSEAFQAR VM VLDAHGVR TN
Sbjct: 61  ANAMWQWNQFVRPLVSHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTN 120

Query: 121 AVGISYGGFVVYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVDEAVSVLLPQS 180
           AVG+SYGGFV YSMAAQFPDR+EKLVLCCTGVCLED+DMEDGMFQVKSV+EAVSVLLPQS
Sbjct: 121 AVGVSYGGFVAYSMAAQFPDRVEKLVLCCTGVCLEDQDMEDGMFQVKSVEEAVSVLLPQS 180

Query: 181 PEKLKEMIQIAFFKPIRIGPTCLVNDLIDELCTEYREQKKELIQALHKERKLSNLPKISQ 240
           PEKLKEMI+IAFFKPIRIGP+CLVNDLIDELCTEYREQKKELIQALHKERKLSNLPKI+ 
Sbjct: 181 PEKLKEMIKIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKLSNLPKITN 240

Query: 241 PTLIMWGEKDLVFPMELAHRLKRHIGDSAELVVIKKAGHALNIEKPKEMNKLIQCFLVDA 300
           PTLIMWGEKDLVFPMELAHRLKRHIG+ AELVVIKKAGHALNIEKPKEMNKLIQCFLVDA
Sbjct: 241 PTLIMWGEKDLVFPMELAHRLKRHIGEGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDA 300

Query: 301 VPSTKAKIHHQNDLKSE 318
           VPSTKAKIHHQNDLKSE
Sbjct: 301 VPSTKAKIHHQNDLKSE 317

BLAST of HG10016545 vs. ExPASy TrEMBL
Match: A0A6J1IMX6 (uncharacterized protein LOC111478485 OS=Cucurbita maxima OX=3661 GN=LOC111478485 PE=4 SV=1)

HSP 1 Score: 604.7 bits (1558), Expect = 2.1e-169
Identity = 291/314 (92.68%), Postives = 301/314 (95.86%), Query Frame = 0

Query: 4   QCFRYGSIMEAWYRSCFSRVGLKSATTDLGNGTVMHCWIPKTPNQTKPNLVLIHGMGANA 63
           QCFRYGSIMEAWYRSCFSRVGL+SATTDLGNGTVMHCWIPKT  +TKPNLVLIHGMGANA
Sbjct: 3   QCFRYGSIMEAWYRSCFSRVGLRSATTDLGNGTVMHCWIPKTRRETKPNLVLIHGMGANA 62

Query: 64  VWQWNKFVRPLIPHFNIYVPDLVFFGESYTTLSDRSEAFQARSVMAVLDAHGVRNTNAVG 123
           VWQWNKFVRPLI HFNIYVPDLVFFGESYTTLSDRSEAFQAR VM VLDAHGVR TNAVG
Sbjct: 63  VWQWNKFVRPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNAVG 122

Query: 124 ISYGGFVVYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVDEAVSVLLPQSPEK 183
           ISYGGFV YSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSV+EAVSVLLPQ+PEK
Sbjct: 123 ISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVEEAVSVLLPQTPEK 182

Query: 184 LKEMIQIAFFKPIRIGPTCLVNDLIDELCTEYREQKKELIQALHKERKLSNLPKISQPTL 243
           LKEMI+IAFFKPIRIGP+CLVNDLIDELCTEYREQKKELIQALHKERK S+LPKI+QPTL
Sbjct: 183 LKEMIRIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKFSDLPKITQPTL 242

Query: 244 IMWGEKDLVFPMELAHRLKRHIGDSAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAVPS 303
           IMWGEKDLVFPMELAHRLK HIGD AELVVIKKAGHALNIEKPKEMNKLIQCFLVDA  S
Sbjct: 243 IMWGEKDLVFPMELAHRLKSHIGDGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAHQS 302

Query: 304 TKAKIHHQNDLKSE 318
           ++AKIHHQND KSE
Sbjct: 303 SQAKIHHQNDPKSE 316

BLAST of HG10016545 vs. ExPASy TrEMBL
Match: A0A6J1GG71 (uncharacterized protein LOC111453865 OS=Cucurbita moschata OX=3662 GN=LOC111453865 PE=4 SV=1)

HSP 1 Score: 604.7 bits (1558), Expect = 2.1e-169
Identity = 291/314 (92.68%), Postives = 301/314 (95.86%), Query Frame = 0

Query: 4   QCFRYGSIMEAWYRSCFSRVGLKSATTDLGNGTVMHCWIPKTPNQTKPNLVLIHGMGANA 63
           QCFRYGSIMEAWYRSCFSRVGL+SATTDLGNGTVMHCWIPKT  +TKPNLVLIHGMGANA
Sbjct: 3   QCFRYGSIMEAWYRSCFSRVGLRSATTDLGNGTVMHCWIPKTRRETKPNLVLIHGMGANA 62

Query: 64  VWQWNKFVRPLIPHFNIYVPDLVFFGESYTTLSDRSEAFQARSVMAVLDAHGVRNTNAVG 123
           VWQWNKFVRPLI HFNIYVPDLVFFGESYTTLSDRSEAFQAR VM VLDAHGVR TNAVG
Sbjct: 63  VWQWNKFVRPLIAHFNIYVPDLVFFGESYTTLSDRSEAFQARCVMGVLDAHGVRTTNAVG 122

Query: 124 ISYGGFVVYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVDEAVSVLLPQSPEK 183
           ISYGGFV YSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSV+EAVSVLLPQ+PEK
Sbjct: 123 ISYGGFVAYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVEEAVSVLLPQTPEK 182

Query: 184 LKEMIQIAFFKPIRIGPTCLVNDLIDELCTEYREQKKELIQALHKERKLSNLPKISQPTL 243
           LKEMI+IAFFKPIRIGP+CLVNDLIDELCTEYREQKKELIQALHKERK S+LPKI+QPTL
Sbjct: 183 LKEMIRIAFFKPIRIGPSCLVNDLIDELCTEYREQKKELIQALHKERKFSDLPKITQPTL 242

Query: 244 IMWGEKDLVFPMELAHRLKRHIGDSAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAVPS 303
           IMWGEKDLVFPMELAHRLK HIGD AELVVIKKAGHALNIEKPKEMNKLIQCFLVDA  S
Sbjct: 243 IMWGEKDLVFPMELAHRLKSHIGDGAELVVIKKAGHALNIEKPKEMNKLIQCFLVDAHQS 302

Query: 304 TKAKIHHQNDLKSE 318
           ++AKIHHQND KSE
Sbjct: 303 SQAKIHHQNDPKSE 316

BLAST of HG10016545 vs. TAIR 10
Match: AT4G39955.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 376.7 bits (966), Expect = 1.8e-104
Identity = 171/294 (58.16%), Postives = 229/294 (77.89%), Query Frame = 0

Query: 5   CFRYGSIMEAWYRSCFSRVGLKSATTDLGNGTVMHCWIPKTPNQTKPNLVLIHGMGANAV 64
           CF Y S     Y+  FSR GL+S+T+DLG+GTV HCWIP T   TKP L+L+HG+GANA+
Sbjct: 4   CFSYVSSRNKCYQYSFSRAGLRSSTSDLGDGTVFHCWIPLTHIHTKPTLLLLHGIGANAM 63

Query: 65  WQWNKFVRPLIPHFNIYVPDLVFFGESYTTLSDRSEAFQARSVMAVLDAHGVRNTNAVGI 124
           WQW++F+   IP FN+YVPDL+FFG+SYTT  DRSE+FQA  VM  +DA+GVR     G+
Sbjct: 64  WQWDRFIDRFIPRFNVYVPDLIFFGDSYTTRPDRSESFQATCVMKAMDAYGVRTMTVAGL 123

Query: 125 SYGGFVVYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVDEAVSVLLPQSPEKL 184
           SYGGFV YS+AAQF +R++++VL C GV LE+KD EDGMF+VKS +EA +VL PQSP  L
Sbjct: 124 SYGGFVAYSLAAQFKERVDRVVLICAGVALEEKDSEDGMFKVKSPEEAAAVLFPQSPSML 183

Query: 185 KEMIQIAFFKPIRIGPTCLVNDLIDELCTEYREQKKELIQALHKERKLSNLPKISQPTLI 244
           + ++Q++F+KP    P+C   D I  +C +Y +++KEL++ALHK R+ +NLPKI+QPTL+
Sbjct: 184 RRLLQLSFYKPPIWIPSCFAMDYIHVMCKDYLQERKELVEALHKGRRFANLPKITQPTLM 243

Query: 245 MWGEKDLVFPMELAHRLKRHIG-DSAELVVIKKAGHALNIEKPKEMNKLIQCFL 298
           +WGE+D VFP+ELAHRLKR++G D A+LV++KK GHA+N EKPKEM K ++ FL
Sbjct: 244 IWGEEDQVFPVELAHRLKRYLGEDRAQLVLLKKTGHAINEEKPKEMYKHMKSFL 297

BLAST of HG10016545 vs. TAIR 10
Match: AT5G09430.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 375.9 bits (964), Expect = 3.1e-104
Identity = 169/303 (55.78%), Postives = 242/303 (79.87%), Query Frame = 0

Query: 4   QCFRYGSIMEAWYRSCFSRVGLKSATTDLGNG-----TVMHCWIPKTPNQTKPNLVLIHG 63
           +CF + +  +  +R  F+  GL+S TTDL +G     T MHCWIPK+PN++KPNL+L+HG
Sbjct: 9   RCFSFTASRDWLFRQSFANAGLRSVTTDLSHGNSIASTAMHCWIPKSPNRSKPNLLLLHG 68

Query: 64  MGANAVWQWNKFVRPLIPHFNIYVPDLVFFGESYTTLSDRSEAFQARSVMAVLDAHGVRN 123
            GANA+WQ+ + +R     FN+YVPDL+FFG S T+  +R+E+FQAR +M +++AHGV+ 
Sbjct: 69  FGANAMWQYGEHLRAFTGRFNVYVPDLLFFGLSSTSEPNRTESFQARCLMRLMEAHGVQR 128

Query: 124 TNAVGISYGGFVVYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVDEAVSVLLP 183
            N VGISYGGFV YS+AAQFP+ +EKLVLCC GVCLE+KDMEDG+F+V +++EA  +L+P
Sbjct: 129 MNIVGISYGGFVGYSLAAQFPENVEKLVLCCAGVCLEEKDMEDGLFKVPNLEEATGILIP 188

Query: 184 QSPEKLKEMIQIAFFKPIRIGPTCLVNDLIDELCTEYREQKKELIQALHKERKLSNLPKI 243
           Q+PEKLKE+I+ +F KPI+  P+  + D ID +CTE+ E+K++LI+++ K+R+LS+LP+I
Sbjct: 189 QTPEKLKELIRFSFVKPIKGVPSFFLWDFIDVMCTEFVEEKRDLIKSILKDRRLSDLPRI 248

Query: 244 SQPTLIMWGEKDLVFPMELAHRLKRHIGDSAELVVIKKAGHALNIEKPKEMNKLIQCFLV 302
            Q +LI+WGE+D +FP+EL +RLKRHIG+SAE+VVIKKAGHA+N+EK KE  K ++ FL+
Sbjct: 249 KQKSLIIWGEEDQIFPLELGYRLKRHIGESAEIVVIKKAGHAVNLEKSKEFVKHLKSFLI 308

BLAST of HG10016545 vs. TAIR 10
Match: AT1G78210.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 317.4 bits (812), Expect = 1.3e-86
Identity = 146/300 (48.67%), Postives = 217/300 (72.33%), Query Frame = 0

Query: 5   CFRYGSIMEAWYRSCFSRVGLKSATTDLGNGTVMHCWIPKT--PNQTKPNLVLIHGMGAN 64
           CF     +E  Y+S F R GL+  T DL +GTV++ W+ KT   ++ KPNL+LIHG+GA 
Sbjct: 4   CFSLSEALERTYKSGFKRSGLRPVTIDLKDGTVVNFWVSKTKPESKPKPNLLLIHGLGAT 63

Query: 65  AVWQWNKFVRPLIPHFNIYVPDLVFFGESYTTLSDRSEAFQARSVMAVLDAHGVRNTNAV 124
           A+WQW    R L  +FN+Y+PDLVFFG S TT  +RS+ FQA+++M  L+A  V+  + V
Sbjct: 64  AIWQWYDVARRLSRYFNLYIPDLVFFGGSSTTRPERSDIFQAQTLMRALEAQSVKKFSLV 123

Query: 125 GISYGGFVVYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVDEAVSVLLPQSPE 184
           G+SYGGFV Y MA+ + D +EK+V+CC  VC+E+KDM+ G+F+V  +DEA  +L+P+S +
Sbjct: 124 GLSYGGFVGYRMASMYADAVEKVVICCAAVCVEEKDMKAGVFKVSDLDEASKILVPESVK 183

Query: 185 KLKEMIQIAFFKP--IRIGPTCLVNDLIDELCT-EYREQKKELIQALHKERKLSNLPKIS 244
           KL+E++   F+KP   R+ PTCL++D I+   T +  E+K+ELI+A+ K+R +S +PK+ 
Sbjct: 184 KLRELMGYIFYKPALARLVPTCLLHDFIEHALTRDNMEEKRELIKAIPKDRIISEIPKLK 243

Query: 245 QPTLIMWGEKDLVFPMELAHRLKRHIGDSAELVVIKKAGHALNIEKPKEMNKLIQCFLVD 300
           QPTLI+WGE D VFP+E+  RL++H+GD+ +LV+IK+ GH  N EKPK+  KL++ FL++
Sbjct: 244 QPTLIIWGEHDQVFPLEMGKRLEKHVGDNGKLVIIKRTGHIFNFEKPKKFIKLLKSFLLE 303

BLAST of HG10016545 vs. TAIR 10
Match: AT5G21950.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 207.6 bits (527), Expect = 1.5e-53
Identity = 118/309 (38.19%), Postives = 172/309 (55.66%), Query Frame = 0

Query: 1   MAGQCFRYGSIMEAWYRSCFSRVGLKSATTDLGNGTVMHCWIP----KTPNQTKPNLVLI 60
           MA         +EA  R  FS  GL   T  + + T +  W P     + N  KP+L+L+
Sbjct: 1   MAPSFLSVARFVEALLRRRFSSAGLSLQTLSIDSETTIQFWGPPPSSSSENTQKPSLLLL 60

Query: 61  HGMGANAVWQWNKFVRPLIPHFNIYVPDLVFFGESYTTLSDRSEAFQARSVMAVLDAHGV 120
           HG G +AVWQW+  V+PL   F +YVPDLVFFG S ++  +RSE FQA  +  +++   V
Sbjct: 61  HGFGPSAVWQWSHQVKPLSHFFRLYVPDLVFFGGSSSSGENRSEMFQALCMGKLMEKLEV 120

Query: 121 RNTNAVGISYGGFVVYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVDEAVSVL 180
              + VG SYGGFV Y+MA  FP+++EK+VL  +GV L   D E  + + K       V+
Sbjct: 121 ERFSVVGTSYGGFVAYNMAKMFPEKVEKVVLASSGVNLRRSDNEAFIARAK-CHRIKEVM 180

Query: 181 LPQSPEKLKEMIQIAFFKPIRIGPTCLVNDLIDELCTEYREQKKELIQALH--KERKLSN 240
           LP S   L+    +   K +   P  ++ND   ++ +E RE+K EL++ L   K+ K +N
Sbjct: 181 LPASATDLRRFSGMVSSKRLDYVPDFVLNDFCQKMYSEKREEKAELLEGLSIGKDDK-TN 240

Query: 241 LPKISQPTLIMWGEKDLVFPMELAHRLKRHIGDSAELVVIKKAGHALNIEKPKEMNKLIQ 300
           +  I Q  +++WGE+D VFP+++AH LK  +G  A L VI+K  H    EK KE N  + 
Sbjct: 241 VSPIQQDVMLIWGEQDQVFPLKMAHDLKEMLGTKATLKVIQKTSHIPQTEKSKEFNGFVM 300

Query: 301 CFLVDAVPS 304
            FL+   PS
Sbjct: 301 SFLLPPSPS 307

BLAST of HG10016545 vs. TAIR 10
Match: AT4G33180.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 188.7 bits (478), Expect = 7.0e-48
Identity = 96/294 (32.65%), Postives = 171/294 (58.16%), Query Frame = 0

Query: 11  IMEAWYRSCFSRVGLKSATTDLGNGTVMHCWIP----KTPNQTKPNLVLIHGMGANAVWQ 70
           ++E + R C    GL S T  + + T +H W P       +  +P ++L+HG G +++WQ
Sbjct: 12  LLEGYLRRCLRAAGLTSQTLSIDSETTIHFWGPPPLDHRSDDDRPVMLLLHGFGPSSMWQ 71

Query: 71  WNKFVRPLIPH-FNIYVPDLVFFGESYTTLSDRSEAFQARSVMAVLDAHGVRNTNAVGIS 130
           W + ++   P  F +Y PDLVFFG+S ++ ++R+E FQA  +  ++   G+   N  G S
Sbjct: 72  WRRQMQAFSPSAFRVYSPDLVFFGDSTSSSTNRTEVFQAECMAKLMAKIGIGKYNVAGTS 131

Query: 131 YGGFVVYSMAAQFPDRMEKLVLCCTGVCLEDKDMEDGMFQVKSVDEAVSVLLPQSPEKLK 190
           YGGFV Y MA  +P+++EK+V+  +G+ +   D E  + Q  + +    V+LP +  + +
Sbjct: 132 YGGFVAYHMAKMWPEKVEKVVIASSGINMRKCDGE-SLLQRSNCECIEKVMLPSTATEFR 191

Query: 191 EMIQIA-FFKPIRIGPTCLVNDLIDELCTEYREQKKELIQALHKERKLS-NLPKISQPTL 250
            ++ +A  ++ +R+ P  L ND+I+ L  + R++K EL++ +   R  + N+  +SQ  L
Sbjct: 192 TLMALASSWRLVRMFPDALWNDVINNLYKKNRKEKIELLKGVTFGRSENLNIDSLSQEVL 251

Query: 251 IMWGEKDLVFPMELAHRLKRHIGDSAELVVIKKAGHALNIEKPKEMNKLIQCFL 298
           I+WG+KD +FP+++A+ LK  +GD  +L +I    H   IE  +E N ++  FL
Sbjct: 252 IVWGDKDQIFPVKMAYELKEILGDKTKLEIIDNTSHVPQIECAQEFNNIVLRFL 304

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038882658.14.9e-17694.642-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase-like [Benincasa hispida][more]
XP_008440587.12.7e-17494.01PREDICTED: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase-like [Cucumis mel... [more]
XP_004143463.11.5e-17292.43uncharacterized protein LOC101208157 [Cucumis sativus] >KGN48741.1 hypothetical ... [more]
XP_022950912.14.4e-16992.68uncharacterized protein LOC111453865 [Cucurbita moschata] >XP_022978531.1 unchar... [more]
XP_023543119.11.7e-16892.36uncharacterized protein LOC111803081 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q525321.4e-1326.152-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Pseu... [more]
Q520364.2e-1325.762-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase OS=Pseudomonas putida OX=303... [more]
P9WNH27.2e-1324.45Uncharacterized protein MT2788 OS=Mycobacterium tuberculosis (strain CDC 1551 / ... [more]
P9WNH37.2e-1324.45Uncharacterized protein Rv2715 OS=Mycobacterium tuberculosis (strain ATCC 25618 ... [more]
P0A5737.2e-1324.45Uncharacterized protein Mb2734 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2... [more]
Match NameE-valueIdentityDescription
A0A5A7SYZ31.3e-17494.012-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase-like OS=Cucumis melo var. ma... [more]
A0A1S3B1G11.3e-17494.012-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase-like OS=Cucumis melo OX=3656... [more]
A0A0A0KLW07.1e-17392.43AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G499... [more]
A0A6J1IMX62.1e-16992.68uncharacterized protein LOC111478485 OS=Cucurbita maxima OX=3661 GN=LOC111478485... [more]
A0A6J1GG712.1e-16992.68uncharacterized protein LOC111453865 OS=Cucurbita moschata OX=3662 GN=LOC1114538... [more]
Match NameE-valueIdentityDescription
AT4G39955.11.8e-10458.16alpha/beta-Hydrolases superfamily protein [more]
AT5G09430.13.1e-10455.78alpha/beta-Hydrolases superfamily protein [more]
AT1G78210.11.3e-8648.67alpha/beta-Hydrolases superfamily protein [more]
AT5G21950.11.5e-5338.19alpha/beta-Hydrolases superfamily protein [more]
AT4G33180.17.0e-4832.65alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 208..232
NoneNo IPR availablePANTHERPTHR43139:SF45ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 3..306
NoneNo IPR availablePANTHERPTHR43139SI:DKEY-122A22.2coord: 3..306
IPR000639Epoxide hydrolase-likePRINTSPR00412EPOXHYDRLASEcoord: 135..148
score: 26.3
coord: 274..296
score: 31.82
coord: 77..92
score: 26.42
coord: 240..256
score: 30.21
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 135..148
score: 41.38
coord: 77..92
score: 29.09
coord: 242..256
score: 41.84
coord: 121..134
score: 34.98
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 51..153
e-value: 7.3E-17
score: 61.9
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 20..310
e-value: 4.0E-48
score: 166.4
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 24..297

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10016545.1HG10016545.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006457 protein folding
biological_process GO:0009408 response to heat
molecular_function GO:0005524 ATP binding
molecular_function GO:0031072 heat shock protein binding
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0051082 unfolded protein binding
molecular_function GO:0003824 catalytic activity