Lag0003131 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0003131 vs. NCBI nr
Match: KAG7013994.1 (E3 ubiquitin-protein ligase ATL6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 226.9 bits (577), Expect = 1.3e-55
Identity = 121/167 (72.46%), Postives = 137/167 (82.04%), Query Frame = 0

Query: 1   MSVIFICSGIAIICSLIIALTFFHLTMVGRCNCGCRSTPTPEPVKNIKHEASNVIQISTI 60
           MSV FICSGIAIICSLII+LTFFHLT++G  NC C S  T E V+NIK+E S+++QI T+
Sbjct: 1   MSVFFICSGIAIICSLIISLTFFHLTILGWFNCCCHSPRTTESVENIKNEVSHIMQIPTV 60

Query: 61  AY-KENENQETNTETECVLCLSPFEDGECIRKLPGCNHVFHASCIDVWLSSHSNCPLCRS 120
            Y KENEN+ETNTE+ECVLCLSPFEDGECIR+LP CNHVFHASCIDVWLSSHS CPLCR+
Sbjct: 61  TYKKENENEETNTESECVLCLSPFEDGECIRQLPSCNHVFHASCIDVWLSSHSECPLCRT 120

Query: 121 PVNHVACNKGESTSEAENSQVVAINNSLQIELS-RVSRREAGPENMV 166
           PV+HVAC     T+EA   QVV I NSLQIELS  V R +A PENMV
Sbjct: 121 PVHHVACR----TAEAVICQVVDI-NSLQIELSPHVIRSKAWPENMV 162

BLAST of Lag0003131 vs. NCBI nr
Match: KAA0059407.1 (E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa] >TYK03920.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa])

HSP 1 Score: 215.3 bits (547), Expect = 3.9e-52
Identity = 115/167 (68.86%), Postives = 131/167 (78.44%), Query Frame = 0

Query: 1   MSVIFICSGIAIICSLIIALTFFHLTMVGRCNCGCRSTPTPEPVKNIKHEASNVIQISTI 60
           MSV+FICSG+AI+CSL IAL FF+L M+G  NC CRS P    VKNIK+E SN+I+ISTI
Sbjct: 68  MSVLFICSGVAIVCSLFIALLFFNLAMLGWFNCCCRS-PEMTDVKNIKYEDSNLIKISTI 127

Query: 61  AY-KENENQETNTETECVLCLSPFEDGECIRKLPGCNHVFHASCIDVWLSSHSNCPLCRS 120
            Y KEN+++ETN  +EC LCLSP EDGECIRKL  CNHVFHASCIDVWL SHSNCPLCR+
Sbjct: 128 MYKKENKSEETNIPSECALCLSPCEDGECIRKLLACNHVFHASCIDVWLYSHSNCPLCRT 187

Query: 121 PVNHVACNKGESTSEAENSQVVAINNSLQIELS-RVSRREAGPENMV 166
            ++HVACN GE TS   NS VV INNSLQI LS   SRR +  ENMV
Sbjct: 188 TLDHVACNNGELTSGVVNSHVVVINNSLQIGLSLHESRRGSPSENMV 233

BLAST of Lag0003131 vs. NCBI nr
Match: KAE8646506.1 (hypothetical protein Csa_015876 [Cucumis sativus])

HSP 1 Score: 205.7 bits (522), Expect = 3.1e-49
Identity = 114/168 (67.86%), Postives = 132/168 (78.57%), Query Frame = 0

Query: 1    MSVIFICSGIAIICSLIIALTFFHLTMV-GRCNCGCRSTPTPEPVKNIKHEASNVIQIST 60
            MSV+FICSGIAIICSL IAL FF+LTM+ G  NC CRS P    VKNIK+E SN+++IST
Sbjct: 914  MSVLFICSGIAIICSLSIALLFFNLTMILGWFNCCCRS-PEMTNVKNIKYEDSNLMKIST 973

Query: 61   IAY-KENENQETNTETECVLCLSPFEDGECIRKLPGCNHVFHASCIDVWLSSHSNCPLCR 120
            I Y KEN+++ETN  +EC LCLSP+EDGECIR+L  CNHVFHASCID+WL SHSNCPLCR
Sbjct: 974  IMYKKENKSEETNITSECALCLSPYEDGECIRELLACNHVFHASCIDIWLYSHSNCPLCR 1033

Query: 121  SPVNHVACNKGESTSEAENSQVVAINNSLQIELS-RVSRREAGPENMV 166
            +P+  VAC  GE TS      VVAI+NSLQIE S   S+REA PENMV
Sbjct: 1034 TPLGRVACINGELTS----GVVVAISNSLQIETSTHGSQREASPENMV 1076

BLAST of Lag0003131 vs. NCBI nr
Match: GAU28792.1 (hypothetical protein TSUD_357780 [Trifolium subterraneum])

HSP 1 Score: 104.8 bits (260), Expect = 7.5e-19
Identity = 60/148 (40.54%), Postives = 84/148 (56.76%), Query Frame = 0

Query: 4   IFICSGIAIICSLIIALTFFHLTMVGRCNCGCRSTPTPEPVKNIKHEASNVIQIS----T 63
           +++  GI II S++IA  FF LTM G CN        P  + +   + S  + +     T
Sbjct: 10  MYVLPGIIIIGSIVIAFIFFRLTMQGWCNLINHEPTLPTTIISYDEKFSGCMSLESHSIT 69

Query: 64  IAYKENE-NQETNTETECVLCLSPFEDGECIRKLPGCNHVFHASCIDVWLSSHSNCPLCR 123
             YKE +  Q+   +TECV+CL+ F++ E +RKL  C HVFH SCID WL SHS CPLCR
Sbjct: 70  FMYKEGDAAQQDINQTECVICLTVFQEDESVRKLHSCKHVFHTSCIDKWLGSHSGCPLCR 129

Query: 124 SPVNHVACNKGESTSEAENSQVVAINNS 147
           S ++ V      + S A +  +VAI +S
Sbjct: 130 SRIDQVT---SPNESLANDHMIVAIVDS 154

BLAST of Lag0003131 vs. NCBI nr
Match: XP_009590813.1 (RING-H2 finger protein ATL52-like [Nicotiana tomentosiformis])

HSP 1 Score: 102.4 bits (254), Expect = 3.7e-18
Identity = 53/151 (35.10%), Postives = 88/151 (58.28%), Query Frame = 0

Query: 10  IAIICSLIIALTFFHLTMVGRCNCGCRSTPTPEP-VKNIKHEASNVIQISTIAYKE---- 69
           + I+   ++ L+ ++L +V  CN    +  + E     + H+  NVI  S+  YK+    
Sbjct: 82  LIIVGIAVVLLSLYNLLIVRWCNENHTNDQSLEQNSSQVSHDNLNVILESSFRYKKGGVE 141

Query: 70  ---NENQETNTETECVLCLSPFEDGECIRKLPGCNHVFHASCIDVWLSSHSNCPLCRSPV 129
              N +++ N + EC +CLS FE+GE +++LP CNH FHASCID+WL SH +CP+CRSPV
Sbjct: 142 VGGNHHEKNNDDIECAVCLSDFEEGEEVKQLPRCNHSFHASCIDMWLYSHLDCPMCRSPV 201

Query: 130 NHVACNKGESTSEAENSQVVAINNSLQIELS 153
                ++   T + E+S+   +  ++Q  +S
Sbjct: 202 EQPVLHRSSFTGQEESSREGIVVQAIQFSVS 232

BLAST of Lag0003131 vs. ExPASy Swiss-Prot
Match: Q9LUZ9 (RING-H2 finger protein ATL63 OS=Arabidopsis thaliana OX=3702 GN=ATL63 PE=2 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 2.8e-16
Identity = 44/86 (51.16%), Postives = 55/86 (63.95%), Query Frame = 0

Query: 37  STPTPEPVKNIKHEASNVI-QISTIAYKENENQETNTETECVLCLSPFEDGECIRKLPGC 96
           S+P     ++ K   S+VI  I    Y+ENE +E + E ECV+CL  +E G+  RKL  C
Sbjct: 99  SSPAATATRDDKGLDSSVISSIPLFVYEENEEEE-DEEEECVICLGLWEAGDFGRKLRNC 158

Query: 97  NHVFHASCIDVWLSSHSNCPLCRSPV 122
            H FH  CID+WLSSHS CPLCRSPV
Sbjct: 159 GHGFHVECIDMWLSSHSTCPLCRSPV 183

BLAST of Lag0003131 vs. ExPASy Swiss-Prot
Match: Q9FLC6 (RING-H2 finger protein ATL73 OS=Arabidopsis thaliana OX=3702 GN=ATL73 PE=2 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.4e-15
Identity = 50/117 (42.74%), Postives = 63/117 (53.85%), Query Frame = 0

Query: 11  AIICSLIIALTFFHLTMVGRC--NCGCRSTPTPEPV-------KNIKHEASNVIQISTIA 70
           A++C+LI AL    +  V RC   C  R TP  +PV       K IK  A  VI +   +
Sbjct: 47  ALLCALICAL---GINSVLRCVLRCTRRFTPNEDPVDTNANVAKGIKKRALKVIPVD--S 106

Query: 71  YKENENQETNTETECVLCLSPFEDGECIRKLPGCNHVFHASCIDVWLSSHSNCPLCR 119
           Y      +    TEC++CL  F +GE +R LP CNH FH  CID WL SHS+CP CR
Sbjct: 107 YSPELKMKA---TECLICLGDFVEGETVRVLPKCNHGFHVKCIDTWLLSHSSCPTCR 155

BLAST of Lag0003131 vs. ExPASy Swiss-Prot
Match: O22755 (Probable E3 ubiquitin-protein ligase ATL44 OS=Arabidopsis thaliana OX=3702 GN=ATL44 PE=1 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.2e-14
Identity = 52/132 (39.39%), Postives = 67/132 (50.76%), Query Frame = 0

Query: 11  AIICSLIIALTFFHLTMVGRCNCGCRST---PTPEPVKNIKHEASNVIQISTIAYKENEN 70
           A++C+LI       L  V RC    R T    +P P K +K +A   +  ST    E+ +
Sbjct: 34  ALLCALICVA---GLAAVVRCAWLRRFTAGGDSPSPNKGLKKKALQSLPRSTFTAAESTS 93

Query: 71  ---QETNTETECVLCLSPFEDGECIRKLPGCNHVFHASCIDVWLSSHSNCPLCRSPVNHV 130
               E    TEC +CL+ F DGE IR LP C H FH  CID WL S S+CP CR  +  V
Sbjct: 94  GAAAEEGDSTECAICLTDFADGEEIRVLPLCGHSFHVECIDKWLVSRSSCPSCRRILTPV 153

Query: 131 ACNKGESTSEAE 137
            C++    S AE
Sbjct: 154 RCDRCGHASTAE 162

BLAST of Lag0003131 vs. ExPASy Swiss-Prot
Match: Q9SRQ8 (RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2)

HSP 1 Score: 80.5 bits (197), Expect = 2.0e-14
Identity = 37/90 (41.11%), Postives = 50/90 (55.56%), Query Frame = 0

Query: 58  STIAYKENENQETNTETECVLCLSPFEDGECIRKLPGCNHVFHASCIDVWLSSHSNCPLC 117
           S   YK  +       ++C +CLS F++ E +R LP CNH FH  CID WL SHSNCPLC
Sbjct: 141 SITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKCNHAFHVPCIDTWLKSHSNCPLC 200

Query: 118 RSPVNHVACNKGESTSEAENSQVVAINNSL 148
           R+    +  +      +  N Q+V  NNS+
Sbjct: 201 RA---FIVTSSAVEIVDLTNQQIVTENNSI 227

BLAST of Lag0003131 vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 3.4e-14
Identity = 40/89 (44.94%), Postives = 48/89 (53.93%), Query Frame = 0

Query: 58  STIAYKENENQETNTETECVLCLSPFEDGECIRKLPGCNHVFHASCIDVWLSSHSNCPLC 117
           S   YK          ++C +CLS FE+ E +R LP CNH FH  CID WL SHSNCPLC
Sbjct: 124 SITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDTWLKSHSNCPLC 183

Query: 118 RSPVNHVACNKGESTSEAENSQVVAINNS 147
           R+ V  V      + S  +N  VV  N S
Sbjct: 184 RAFVTGV---NNPTASVGQNVSVVVANQS 209