Lag0019939 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0019939
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionzinc finger A20 and AN1 domain-containing stress-associated protein 5-like
Locationchr5: 46847133 .. 46847642 (-)
RNA-Seq ExpressionLag0019939
SyntenyLag0019939
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCTTTTGGAAGCTCAAAGAATGAAAACGACGCACCACTCTGCGCCAACAACTGTGGCTTCTATGGAAATCCGAAGAATCACAACTTGTGCTCCGTTTGCTACGCCGCTTTCCTCAGAGAAACCGGCGAAGATTCTCGACGACGAGAGAGCTCAAACGATGTCATTCGCTCAAAAAATCTGATCAGTGCAGAACTCGTTGAAACTCGTGATGCAGAACCAAATCTCGGAACGAGACGATCTCCGGCGGAAGCGGAGAGTTCCGATACTTCTGAATCGGTCGCCGATTTGAAGAATCCGGCGCCTAAAACCAAGAATAGATGCAAAATCTGCAAGAAGAAGGTCGGATTGTTGGGATTCAACTGCCGGTGTGGAGGTTCGTTCTGCGGTAAACATAGGCATCCTGAAGAGCATTCGTGCGACGTTGATCACAAGGTGATCGCCCGAGAGATTTTGGCGAAGCAAATGGTCGAATGCAAGGCCGATAAATTGGAATTTAGGGTTTAG

mRNA sequence

ATGGCTGCTTTTGGAAGCTCAAAGAATGAAAACGACGCACCACTCTGCGCCAACAACTGTGGCTTCTATGGAAATCCGAAGAATCACAACTTGTGCTCCGTTTGCTACGCCGCTTTCCTCAGAGAAACCGGCGAAGATTCTCGACGACGAGAGAGCTCAAACGATGTCATTCGCTCAAAAAATCTGATCAGTGCAGAACTCGTTGAAACTCGTGATGCAGAACCAAATCTCGGAACGAGACGATCTCCGGCGGAAGCGGAGAGTTCCGATACTTCTGAATCGGTCGCCGATTTGAAGAATCCGGCGCCTAAAACCAAGAATAGATGCAAAATCTGCAAGAAGAAGGTCGGATTGTTGGGATTCAACTGCCGGTGTGGAGGTTCGTTCTGCGGTAAACATAGGCATCCTGAAGAGCATTCGTGCGACGTTGATCACAAGGTGATCGCCCGAGAGATTTTGGCGAAGCAAATGGTCGAATGCAAGGCCGATAAATTGGAATTTAGGGTTTAG

Coding sequence (CDS)

ATGGCTGCTTTTGGAAGCTCAAAGAATGAAAACGACGCACCACTCTGCGCCAACAACTGTGGCTTCTATGGAAATCCGAAGAATCACAACTTGTGCTCCGTTTGCTACGCCGCTTTCCTCAGAGAAACCGGCGAAGATTCTCGACGACGAGAGAGCTCAAACGATGTCATTCGCTCAAAAAATCTGATCAGTGCAGAACTCGTTGAAACTCGTGATGCAGAACCAAATCTCGGAACGAGACGATCTCCGGCGGAAGCGGAGAGTTCCGATACTTCTGAATCGGTCGCCGATTTGAAGAATCCGGCGCCTAAAACCAAGAATAGATGCAAAATCTGCAAGAAGAAGGTCGGATTGTTGGGATTCAACTGCCGGTGTGGAGGTTCGTTCTGCGGTAAACATAGGCATCCTGAAGAGCATTCGTGCGACGTTGATCACAAGGTGATCGCCCGAGAGATTTTGGCGAAGCAAATGGTCGAATGCAAGGCCGATAAATTGGAATTTAGGGTTTAG

Protein sequence

MAAFGSSKNENDAPLCANNCGFYGNPKNHNLCSVCYAAFLRETGEDSRRRESSNDVIRSKNLISAELVETRDAEPNLGTRRSPAEAESSDTSESVADLKNPAPKTKNRCKICKKKVGLLGFNCRCGGSFCGKHRHPEEHSCDVDHKVIAREILAKQMVECKADKLEFRV
Homology
BLAST of Lag0019939 vs. NCBI nr
Match: KAG7014809.1 (Zinc finger A20 and AN1 domain-containing stress-associated protein 10, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 245.0 bits (624), Expect = 4.7e-61
Identity = 121/169 (71.60%), Postives = 133/169 (78.70%), Query Frame = 0

Query: 1   MAAFGSSKNENDAPLCANNCGFYGNPKNHNLCSVCYAAFLRETGEDSRRRESSNDVIRSK 60
           MA FGSSKN+NDAPLCANNCGFYG+PKN NLCS CYAAFL+ETGE+S R E+SN  I   
Sbjct: 1   MAGFGSSKNDNDAPLCANNCGFYGSPKNRNLCSFCYAAFLKETGENSERLENSNHRI--- 60

Query: 61  NLISAELVETRDAEPNLGTRRSPAEAESSDTSESVADLKNPAPKTKNRCKICKKKVGLLG 120
                     R+ EPNL  RR P   +SS+TSE VADL NPA KTK RCKICKKK+G+LG
Sbjct: 61  ----------REEEPNLRKRRFPWAGQSSETSEPVADLNNPANKTKIRCKICKKKIGMLG 120

Query: 121 FNCRCGGSFCGKHRHPEEHSCDVDHKVIAREILAKQMVECKADKLEFRV 170
           FNCRCGG FC KHRHPEEHSCDVDHK I R+ILAK +VECKADKLEFRV
Sbjct: 121 FNCRCGGPFCCKHRHPEEHSCDVDHKAIGRQILAKHIVECKADKLEFRV 156

BLAST of Lag0019939 vs. NCBI nr
Match: XP_022141121.1 (zinc finger A20 and AN1 domain-containing stress-associated protein 5-like [Momordica charantia])

HSP 1 Score: 224.2 bits (570), Expect = 8.7e-55
Identity = 117/172 (68.02%), Postives = 127/172 (73.84%), Query Frame = 0

Query: 7   SKNENDAPLCANNCGFYGNPKNHNLCSVCYAAFLRETGEDSRRRESSNDVIRSKNLISAE 66
           S + +DA LCANNCGFYG PKN NLCSVCYAA L+ETGEDS RRESS+DV+       AE
Sbjct: 4   SGSASDAKLCANNCGFYGTPKNRNLCSVCYAASLKETGEDSGRRESSHDVVP-----GAE 63

Query: 67  LVETRDAEPNLGTRR---------SPAEAESSDTSESVADLKNPAPKTKNRCKICKKKVG 126
           LV   D  P+ GTR          S +E+ SS TS S A   NPAPKTKNRC ICKKKVG
Sbjct: 64  LVGVHDEAPDFGTRESSPPAPASASASESTSSATSGSEAGSSNPAPKTKNRCTICKKKVG 123

Query: 127 LLGFNCRCGGSFCGKHRHPEEHSCDVDHKVIAREILAKQMVECKADKLEFRV 170
           LLGFNCRCGG FCG+HRHPEEHSC VD K I REIL KQ+VECKADKLEFRV
Sbjct: 124 LLGFNCRCGGLFCGRHRHPEEHSCGVDRKAIGREILEKQIVECKADKLEFRV 170

BLAST of Lag0019939 vs. NCBI nr
Match: XP_038876927.1 (zinc finger A20 and AN1 domain-containing stress-associated protein 10-like [Benincasa hispida])

HSP 1 Score: 217.6 bits (553), Expect = 8.1e-53
Identity = 111/169 (65.68%), Postives = 128/169 (75.74%), Query Frame = 0

Query: 1   MAAFGSSKNENDAPLCANNCGFYGNPKNHNLCSVCYAAFLRETGEDSRRRESSNDVIRSK 60
           MAAF SSKN  DAPLCANNCGFY NP N NLCSV YAAFL+ETGEDS  RESS   I+ +
Sbjct: 1   MAAFRSSKNGGDAPLCANNCGFYENPNNLNLCSVYYAAFLKETGEDSEPRESSKSQIKYE 60

Query: 61  NLISAELVETRDAEPNLGTRRSPAEAESSDTSESVADLKNPAPKTKNRCKICKKKVGLLG 120
                        EPN  TR+ P+ +++S+T E+     NP PKT NRCKIC+KKVGLLG
Sbjct: 61  -------------EPNSETRQFPSVSQNSETCEN-----NPTPKTINRCKICRKKVGLLG 120

Query: 121 FNCRCGGSFCGKHRHPEEHSCDVDHKVIAREILAKQMVECKADKLEFRV 170
           FNC+CGGSFCGKHR+PEEHSCDVDHK I R+ LAKQ+VECKA+KL+FRV
Sbjct: 121 FNCQCGGSFCGKHRYPEEHSCDVDHKAIGRKTLAKQIVECKANKLQFRV 151

BLAST of Lag0019939 vs. NCBI nr
Match: KAG6576775.1 (Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 197.2 bits (500), Expect = 1.1e-46
Identity = 97/141 (68.79%), Postives = 107/141 (75.89%), Query Frame = 0

Query: 1   MAAFGSSKNENDAPLCANNCGFYGNPKNHNLCSVCYAAFLRETGEDSRRRESSNDVIRSK 60
           MA FGSS N+NDAPLCANNCGFYG+PKN NLCS CYAAFL+ETGE+S R E+SN  I   
Sbjct: 862 MAGFGSSNNDNDAPLCANNCGFYGSPKNRNLCSFCYAAFLKETGENSERLENSNHRI--- 921

Query: 61  NLISAELVETRDAEPNLGTRRSPAEAESSDTSESVADLKNPAPKTKNRCKICKKKVGLLG 120
                     R+ EPNL  RR P   +SS+TSE VADL NPA KTK RCKICKKK+G+LG
Sbjct: 922 ----------REEEPNLRKRRFPWAGQSSETSEPVADLNNPANKTKIRCKICKKKIGMLG 981

Query: 121 FNCRCGGSFCGKHRHPEEHSC 142
           FNCRCGG FC KHRHPEEHSC
Sbjct: 982 FNCRCGGPFCCKHRHPEEHSC 989

BLAST of Lag0019939 vs. NCBI nr
Match: XP_031739041.1 (AN1-type zinc finger protein 6-like [Cucumis sativus])

HSP 1 Score: 187.2 bits (474), Expect = 1.2e-43
Identity = 99/170 (58.24%), Postives = 118/170 (69.41%), Query Frame = 0

Query: 1   MAAFGSSKNENDAPLCANNCGFYGNPKNHNLCSVCYAAFLRETGEDSRRRESSNDVIRSK 60
           MA F SSKN ND  LCANNCGFYGNP N NLCSVCYAAFL+ETG     R++S     SK
Sbjct: 1   MADFESSKNGNDPALCANNCGFYGNPNNRNLCSVCYAAFLKETGAKYFERQNS-----SK 60

Query: 61  NLISAELVETRDAEPNLGTRRSPAEAESSDTSESVADLKNPA-PKTKNRCKICKKKVGLL 120
           + I            NL TR+S +     +      D  +PA PKT+NRC+IC+KKVG++
Sbjct: 61  SQI------------NLETRQSSSFGVLENL--ETCDHNDPAPPKTQNRCEICQKKVGMI 120

Query: 121 GFNCRCGGSFCGKHRHPEEHSCDVDHKVIAREILAKQMVECKADKLEFRV 170
           GF+CRCGG FCGKHR+PEEHSC  DHK + R ILAKQ+VECKADKL+FR+
Sbjct: 121 GFSCRCGGCFCGKHRYPEEHSCGFDHKEVGRNILAKQIVECKADKLKFRI 151

BLAST of Lag0019939 vs. ExPASy Swiss-Prot
Match: Q6NNI8 (Zinc finger A20 and AN1 domain-containing stress-associated protein 1 OS=Arabidopsis thaliana OX=3702 GN=SAP1 PE=1 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.4e-23
Identity = 62/161 (38.51%), Postives = 88/161 (54.66%), Query Frame = 0

Query: 6   SSKNENDAPLCANNCGFYGNPKNHNLCSVCYAAFLRETGEDSRRRESSNDVIRSKNLISA 65
           +S + ++  LC   CGF+G+P N NLCS CY   +R T E +   +++  V +S N    
Sbjct: 9   TSFSPSEPKLCVKGCGFFGSPSNMNLCSKCYRD-IRATEEQTASAKAA--VEKSLNPNKP 68

Query: 66  ELVETRDAEPNLGTRRSPAEAESSDTSESVADLKNPAPKTKNRCKICKKKVGLLGFNCRC 125
           +    +  E   G   S + + S+   +S A   +P   T  RC  C KKVG+ GF CRC
Sbjct: 69  KTQPQQSQEITQGVLGSGSSSSSTRGGDSAAAPLDPPKSTATRCLSCNKKVGVTGFKCRC 128

Query: 126 GGSFCGKHRHPEEHSCDVDHKVIAREILAKQMVECKADKLE 167
           G +FCG HR+PE H C  D K +ARE +AK     KADK++
Sbjct: 129 GSTFCGTHRYPESHECQFDFKGVAREAIAKANPVVKADKVD 166

BLAST of Lag0019939 vs. ExPASy Swiss-Prot
Match: Q852K6 (Zinc finger A20 and AN1 domain-containing stress-associated protein 7 OS=Oryza sativa subsp. japonica OX=39947 GN=SAP7 PE=2 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 5.9e-22
Identity = 60/160 (37.50%), Postives = 79/160 (49.38%), Query Frame = 0

Query: 13  APLCANNCGFYGNPKNHNLCSVCYAAFLRETGEDSRRRESSNDVIRSKNLISAELVETRD 72
           A +C   CGF+G+   +N+CS CY           R   + ND +      +++L     
Sbjct: 21  AAMCVTGCGFFGSEATNNMCSRCY-----------REHSADNDAVEEAAAANSDLELVGV 80

Query: 73  AEPNLGTRRSPAEAESSDTSESVADLKNPAPK---TKNRCKICKKKVGLLGFNCRCGGSF 132
           AE      R  A    +  S S A  + PA K     NRC  C+KKVGL GF CRCGG+F
Sbjct: 81  AETTTKKARMSAVVPVAVASSSSAAAEQPAAKAATAPNRCAACRKKVGLTGFKCRCGGNF 140

Query: 133 CGKHRHPEEHSCDVDHKVIAREILAKQMVECKADKLEFRV 170
           CG HRH + H C  D+K   +E +AKQ     ADKL  R+
Sbjct: 141 CGGHRHADAHGCGFDYKSAGKEQIAKQNPLVVADKLATRI 169

BLAST of Lag0019939 vs. ExPASy Swiss-Prot
Match: Q5JN07 (Zinc finger A20 and AN1 domain-containing stress-associated protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=SAP3 PE=2 SV=2)

HSP 1 Score: 104.4 bits (259), Expect = 1.3e-21
Identity = 57/156 (36.54%), Postives = 84/156 (53.85%), Query Frame = 0

Query: 15  LCANNCGFYGNPKNHNLCSVCYAAFLRETGEDSRRRESSNDVIRSKNLISAELVETRDAE 74
           LCA+ CGFYGNP   ++CSVCY       G       SS+    S   ++   V +    
Sbjct: 204 LCASGCGFYGNPATLDMCSVCYRQHCLLNGATMATGPSSSVAAASAATVATGAVTSDSCS 263

Query: 75  -PNLGTRRSPAEAESSDTSESVADLKNPAPKTKNRCKICKKKVGLLGFNCRCGGSFCGKH 134
            P+     +   ++++    +V + K PA    NRC  CKKKVGLLGF CRCG ++CG H
Sbjct: 264 VPSAEVNGAAFSSKNNPEPATVVEKKAPA----NRCASCKKKVGLLGFACRCGATYCGTH 323

Query: 135 RHPEEHSCDVDHKVIAREILAKQMVECKADKLEFRV 170
           R+PE+H+C  D K  +R+ +A+     K +KL  ++
Sbjct: 324 RYPEKHACGFDFKGASRDAIARANPLIKGEKLTNKI 355

BLAST of Lag0019939 vs. ExPASy Swiss-Prot
Match: Q852K5 (Zinc finger A20 and AN1 domain-containing stress-associated protein 6 OS=Oryza sativa subsp. japonica OX=39947 GN=SAP6 PE=2 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 8.5e-21
Identity = 64/161 (39.75%), Postives = 86/161 (53.42%), Query Frame = 0

Query: 12  DAP-LCANNCGFYGNPKNHNLCSVCYAAFLRETGEDSRRRESSNDVIRSKNLISAELVET 71
           +AP LC NNCGF+G+    N+CS CY                  D +++K +  A +VE 
Sbjct: 19  EAPILCVNNCGFFGSRMTENMCSKCY-----------------RDTVKAKTV--ATVVEK 78

Query: 72  RDAEPNLGTRRSPAEAESSD-TSESVAD----LKNPAPK-TKNRCKICKKKVGLLGFNCR 131
           +   P      +P   E +D  S SVAD    ++   PK   NRC  C+KKVGL GF CR
Sbjct: 79  K---PLASLSSTPLVTEVTDGGSGSVADGKQVMEEDTPKPPSNRCLSCRKKVGLTGFKCR 138

Query: 132 CGGSFCGKHRHPEEHSCDVDHKVIAREILAKQMVECKADKL 166
           CGG+FC  HR+ + H C  D+K + RE +AKQ    KADK+
Sbjct: 139 CGGTFCSMHRYADSHKCTFDYKQVGREQIAKQNPLVKADKI 157

BLAST of Lag0019939 vs. ExPASy Swiss-Prot
Match: Q9SZ69 (Zinc finger A20 and AN1 domain-containing stress-associated protein 7 OS=Arabidopsis thaliana OX=3702 GN=SAP7 PE=1 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 8.5e-21
Identity = 59/172 (34.30%), Postives = 93/172 (54.07%), Query Frame = 0

Query: 5   GSSKNEN------DAPLCANNCGFYGNPKNHNLCSVCYAAFLRETGEDSRRRESSNDVIR 64
           GS +N +      +  LC N CGF+G+P N NLCS CY +   E  + +  + +  + ++
Sbjct: 2   GSEENNSTSFPPTEPKLCDNGCGFFGSPSNMNLCSKCYRSLRAEEDQTAVAKAAVKNSLK 61

Query: 65  --SKNLISAELVETRDAEP-NLGTRRSPAEAESSDTSESVADLKNPAP-KTKNRCKICKK 124
             S ++I+       + +P +L T    AE  S   +    + +   P +  NRC  C K
Sbjct: 62  LPSCSIIAPGQKHPLEIKPAHLETVVVTAEPSSVPVAAEQDEAEPSRPVRPNNRCFSCNK 121

Query: 125 KVGLLGFNCRCGGSFCGKHRHPEEHSCDVDHKVIAREILAKQMVECKADKLE 167
           KVG++GF C+CG +FCG HR+PE+H C  D K + R+ +AK     KADK++
Sbjct: 122 KVGVMGFKCKCGSTFCGSHRYPEKHECSFDFKEVGRDAIAKANPLVKADKVQ 173

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7014809.14.7e-6171.60Zinc finger A20 and AN1 domain-containing stress-associated protein 10, partial ... [more]
XP_022141121.18.7e-5568.02zinc finger A20 and AN1 domain-containing stress-associated protein 5-like [Momo... [more]
XP_038876927.18.1e-5365.68zinc finger A20 and AN1 domain-containing stress-associated protein 10-like [Ben... [more]
KAG6576775.11.1e-4668.79Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita a... [more]
XP_031739041.11.2e-4358.24AN1-type zinc finger protein 6-like [Cucumis sativus][more]
Match NameE-valueIdentityDescription
Q6NNI81.4e-2338.51Zinc finger A20 and AN1 domain-containing stress-associated protein 1 OS=Arabido... [more]
Q852K65.9e-2237.50Zinc finger A20 and AN1 domain-containing stress-associated protein 7 OS=Oryza s... [more]
Q5JN071.3e-2136.54Zinc finger A20 and AN1 domain-containing stress-associated protein 3 OS=Oryza s... [more]
Q852K58.5e-2139.75Zinc finger A20 and AN1 domain-containing stress-associated protein 6 OS=Oryza s... [more]
Q9SZ698.5e-2134.30Zinc finger A20 and AN1 domain-containing stress-associated protein 7 OS=Arabido... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002653Zinc finger, A20-typeSMARTSM00259A20_3coord: 13..37
e-value: 2.2E-7
score: 40.5
IPR002653Zinc finger, A20-typePFAMPF01754zf-A20coord: 15..36
e-value: 2.9E-10
score: 39.9
IPR002653Zinc finger, A20-typePROSITEPS51036ZF_A20coord: 10..44
score: 9.501081
IPR000058Zinc finger, AN1-typeSMARTSM00154AN1_Zf_4coord: 109..146
e-value: 1.9E-14
score: 64.0
IPR000058Zinc finger, AN1-typePFAMPF01428zf-AN1coord: 109..145
e-value: 3.7E-10
score: 39.8
IPR000058Zinc finger, AN1-typePROSITEPS51039ZF_AN1coord: 106..149
score: 11.865503
NoneNo IPR availableGENE3D1.20.5.4770coord: 5..59
e-value: 5.0E-16
score: 60.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 82..98
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 67..100
NoneNo IPR availablePANTHERPTHR10634:SF124ZINC FINGER A20 AND AN1 DOMAIN-CONTAINING STRESS-ASSOCIATED PROTEIN 8-RELATEDcoord: 11..169
NoneNo IPR availablePANTHERPTHR10634AN1-TYPE ZINC FINGER PROTEINcoord: 11..169
NoneNo IPR availableSUPERFAMILY57716Glucocorticoid receptor-like (DNA-binding domain)coord: 15..51
IPR035896AN1-like Zinc fingerGENE3D4.10.1110.10coord: 82..163
e-value: 2.2E-23
score: 83.9
IPR035896AN1-like Zinc fingerSUPERFAMILY118310AN1-like Zinc fingercoord: 94..154

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0019939.1Lag0019939.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003677 DNA binding
molecular_function GO:0008270 zinc ion binding