Lsi09G003840 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G003840
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionCytochrome P450 76M5-like
Locationchr09: 4044885 .. 4048741 (-)
RNA-Seq ExpressionLsi09G003840
SyntenyLsi09G003840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATTAATGGGTTATATTCGCTTTTGTTTGAAGTAATTTTTTTTATTATCTAATTTTTCACCAACTTATTAACTACTGGAACATTTAATTTTACCCTAGGTTAAAATATGATTTTTTTTTCCTTCCTTGAGAAAATGATTGATGCCCTTTTGGAAGGTAAAGGCAGAGCCATAGATTCCTCATCTTGTCTTGTCTCCACTGGATTTTCTTTATGCCAATCTTCGTCAACATTACAAAACTTAGCCAAATTCTCATTCTTTCTCCACTCCGCCATGGAACAACCCACCTCCAATTCTCTTCTACATCTCATTGATTCCCTCAATTCCTTCTTCCCATGGCGCTCCATTGACCAATCCCGCTTCCTCTTCACCTTCTTCGCCGCTATTCTAATTTTCCTCTATATAAAACTCAAGCGCCGCCGCCTGCCACTTCCACCAGGTCCCCGAGGCGTTCCACTCCTCGGAAACCTCCCGTTTCTCGACCCTGAACTTCACACATACTTCACCAAACTGGGTGAAAAATACGGCCCAATAGTAAAGCTTCAACTTGGCAAAAAAATCGGTATAATCGTAAATTCACCGTCAGTAGCACGTGAGGTGTTGAAAGACCAAGATGTCACGTTTGCCAATCGCGATGTTCCTCAAGCTGGGAGGGCCGCCACGTACGGTGGTTCCGACATAGTTTGGACACCGTACGGACCTGAGTGGCGAATGTTGAGAAAAGTTTGCGTGTTAAAGATGTTGAGTAACACCACTTTGGACAGCGTTTACGAACTCCGCTATAGAGAGGTGAGAAACACGGTGGCTCACTTGTACCGGCGATCTGGGTCGGCAGTGAACGTGGGAGAGCAGGGGTTTTTGACGATTTTCAACGTGGTTACAAGTATGTTGTGGGGTGGGTCAGTGGAAGGGGAGCAGAGGGATGGTCTTGCGGCAGAGTTTAGAGAGACGGTTTCGGAAATAACAGAGCTATTGGGAAAGCCTAATGTTTCGGATTTTTTTCCGAGTTTGGCCCGTTTTGATCTCCAAGGGATTGAGAAGCAGATGCGTGAGATTGCTCCAAGATTTGATAACATTTTCGAGAAGATGATTGATCAACGATTGAAAATGGGTAATGAAGACGAGGACGGGAATGTCAAGAAAAATGATTTCTTGCAGTTCTTACTTAAAGTCAAAGATGAAGGAGAATCCAAGACTCCTCTCACCATGACCCACCTTAAAGCCTTGCTCATGGTAACTAACTTTAACACACTCTTTTTTATGTTATATTATAAATTTATTATTTTTATTTCCAAGGTTAGAATTGGGTTAAAATATTATTTTGGTATTTATACTTTAAGATTTATTACATTTTGATCTTTTACGAGTCTAATTTTAAACATATTTTCAATAGATCTTAAAATTAGTCCATAAAATTAGTTTATTGTTGATTTTTTTAAAAAAAAAGAAGTTCTATTAATATTTTGTTTATATATTTGTTTCTATTAGCATTCTCCTCAATCTCCCGTTTCTCTTTAATATTTGTTTACTATGGTTTTCATCTTTGAATTATATAATAATAATAATAATATAATTGTTTTAATTTCTAAACTTGATTTGAAATTTGAAAACACTCCAAAAGAGTAGATACAAAACAAAGGAACTAGTATAAAGTATTGTTTACAAACTTCAGTTTAAAAAATAGATATTAAAAATATTAAAGCTTCATTTGACATCTATTTGATTTTTCATTTTGTGTTTTCGAAAATTGTGCTTGTATTCTTATGATTAAGATTTTCATCTTTCTAATTGATTTATTTTTAGTTTTCAAATGTTAGTTTGGATTTTGAAAACACTACTTCTAAAATGTAAATAATAAAGCATAGATGATAAGCATCCAAAGCTAGAAGTCTTTTAGGATCCTTGCAAATACTCACAAATAAGGTTAATTTAATGAATGATTCCAACCAAAAGCTTATAGTTATAAGGTTGAATAACTCTTAGATCAAATTTAGAATCTATGATGATACTCTTAGATTCTAAGACAATTCACTCTAGAATTAGTTTGATCAAAACTAATTCAATGAATCTATTTAATCATCAAATCTAAAACAAGATTTAAAAAAAGACAACACGAAAAAAGTTGAAGCAACACAATGTTTGGATATCATTAATATGCAAGTATTGCATATGATTTTAAAGGCCAAATAGATGTTCAAAGGGCTAAAAAATTGAAACTCAATTTCACATCAGTTGAGAAGTCAAAATACAAAACTAAAAATGTAAAATTGCTTCGAATCGTCTTAAATATCTTATCAAGAAGGCAGTAAGCTTGATGTGCACGTTTGAGAAGGTTTGACATAACAAATTCTAACACAGTGCACACTTGACTACTTCTTTTACAAGTTTTTTTTTTTTGTCTTCTGTGCCATCTTCCAAACTATACTACTTTTCTCGTGCCATCTTCATTCCATTATCTTCCAGTCTCTCATTGAATTAATATTATAGATAAACTTAGGTTTAAAAATCTTTGTCTCTTCTTGTACATTAGCTAGCCTTTGAAGATGTAATGTGAAAGCTTCCTAAATTTTTTTTGCATTGATTCTGGTAATAACTCCTTTAGCTACATTCAACAAGTCAATGGTTGAAATCACATCAATAGAAATCAATAAATGAAAAGAATGGTTTAAGCTTAAATTTTGAAAACTAAAAACAAAACAACCCACCCGTAGTTATCGAACGGAGCTTAAATAGTTTTCAAATGAAGCTTTAATTTTTAAAAATTAATTTATAGGCACTACTAAACCTTAATTGATTAAGTTCATGAATGGAACTTGTAGCTAAACATTAATTTTATTAAAGTATAATAGTGAATTAAGAAATTCAAGTTTTTCAAATGAATATTAATGAAATGCTCTGTGTGGGGGCAGGATATGGTGGTCGGCGGGACAGACACATCGTCAAATACCGTAGAATTTGCGATGGCAGAAATGATAAAGAACCCAAAAACTCTAAAGAAAGCACAAGAAGAAATCACCGCCGTAGTCGGAGAAGACAACATCGTGGAAGAATCCCACATTCATAGTTTCCCATATCTCAAAGCCGTAATGAAAGAAACTTTACGTTTACACCCAATTCTACCTCTGCTCGTGCCGCATTGCCCAAGCGAGACCACCATCGTCTCCAATTACGCAATTCCAAAGGGCTCTCGAGTGTTCATCAACGTCTGGGCCATTCAGCGAGACCCCAATCAATGGGAAAATCCATTGGTATTTGAACCAGAGAGATTTCTTGAGACCGACAAGTGGGATTTTGGTGGGAGTGATTTCCGTTATTTCCCATTTGGGTCCGGCAGAAGAAACTGTGCAGGGATAGCCATGGCGGAAAGAATGGTAATTTATTTGCTTGCTACGCTTTTGCATTCTTTTGATTGGAAATTGGAAGATGGTGAGAAGATGGAAGTTGAGGAGAAATTTGGGATTGTTATGAAGATGAAGTCGCCTCTCGTTCTCATTCCAACGCCAAGACTATCTGATCCCACTCTGTATCAGTAAGCTGATTGCCATTGTTGTTCCTTTCAAAGCTAGCAAAGTATATATATGCTCATGCTCACACTATATGATACTAGTTAATATTTGCACTTTTATTCTTTGATGATGTAAAGCTTTAAATTTTATATTTATGGTATACAACTCTAAATCAATATGCTTAAAGGTTGTATAGATATCGAATTCAAGAGAAATAAATTTCATTCTCATGTTAAATTTAGATAGATCACATTTTTCTATTAGTTTTTTTTTGCACATTTTATAACAAGAAGAAGCAATTCTGTCTGATGTTGATGAATGAAATACTAACCGTGAAATAGTAATATACAGGA

mRNA sequence

TATTAATGGGTTATATTCGCTTTTGTTTGAAGTAATTTTTTTTATTATCTAATTTTTCACCAACTTATTAACTACTGGAACATTTAATTTTACCCTAGGTTAAAATATGATTTTTTTTTCCTTCCTTGAGAAAATGATTGATGCCCTTTTGGAAGGTAAAGGCAGAGCCATAGATTCCTCATCTTGTCTTGTCTCCACTGGATTTTCTTTATGCCAATCTTCGTCAACATTACAAAACTTAGCCAAATTCTCATTCTTTCTCCACTCCGCCATGGAACAACCCACCTCCAATTCTCTTCTACATCTCATTGATTCCCTCAATTCCTTCTTCCCATGGCGCTCCATTGACCAATCCCGCTTCCTCTTCACCTTCTTCGCCGCTATTCTAATTTTCCTCTATATAAAACTCAAGCGCCGCCGCCTGCCACTTCCACCAGGTCCCCGAGGCGTTCCACTCCTCGGAAACCTCCCGTTTCTCGACCCTGAACTTCACACATACTTCACCAAACTGGGTGAAAAATACGGCCCAATAGTAAAGCTTCAACTTGGCAAAAAAATCGGTATAATCGTAAATTCACCGTCAGTAGCACGTGAGGTGTTGAAAGACCAAGATGTCACGTTTGCCAATCGCGATGTTCCTCAAGCTGGGAGGGCCGCCACGTACGGTGGTTCCGACATAGTTTGGACACCGTACGGACCTGAGTGGCGAATGTTGAGAAAAGTTTGCGTGTTAAAGATGTTGAGTAACACCACTTTGGACAGCGTTTACGAACTCCGCTATAGAGAGGTGAGAAACACGGTGGCTCACTTGTACCGGCGATCTGGGTCGGCAGTGAACGTGGGAGAGCAGGGGTTTTTGACGATTTTCAACGTGGTTACAAGTATGTTGTGGGGTGGGTCAGTGGAAGGGGAGCAGAGGGATGGTCTTGCGGCAGAGTTTAGAGAGACGGTTTCGGAAATAACAGAGCTATTGGGAAAGCCTAATGTTTCGGATTTTTTTCCGAGTTTGGCCCGTTTTGATCTCCAAGGGATTGAGAAGCAGATGCGTGAGATTGCTCCAAGATTTGATAACATTTTCGAGAAGATGATTGATCAACGATTGAAAATGGGTAATGAAGACGAGGACGGGAATGTCAAGAAAAATGATTTCTTGCAGTTCTTACTTAAAGTCAAAGATGAAGGAGAATCCAAGACTCCTCTCACCATGACCCACCTTAAAGCCTTGCTCATGGATATGGTGGTCGGCGGGACAGACACATCGTCAAATACCGTAGAATTTGCGATGGCAGAAATGATAAAGAACCCAAAAACTCTAAAGAAAGCACAAGAAGAAATCACCGCCGTAGTCGGAGAAGACAACATCGTGGAAGAATCCCACATTCATAGTTTCCCATATCTCAAAGCCGTAATGAAAGAAACTTTACGTTTACACCCAATTCTACCTCTGCTCGTGCCGCATTGCCCAAGCGAGACCACCATCGTCTCCAATTACGCAATTCCAAAGGGCTCTCGAGTGTTCATCAACGTCTGGGCCATTCAGCGAGACCCCAATCAATGGGAAAATCCATTGGTATTTGAACCAGAGAGATTTCTTGAGACCGACAAGTGGGATTTTGGTGGGAGTGATTTCCGTTATTTCCCATTTGGGTCCGGCAGAAGAAACTGTGCAGGGATAGCCATGGCGGAAAGAATGGTAATTTATTTGCTTGCTACGCTTTTGCATTCTTTTGATTGGAAATTGGAAGATGGTGAGAAGATGGAAGTTGAGGAGAAATTTGGGATTGTTATGAAGATGAAGTCGCCTCTCGTTCTCATTCCAACGCCAAGACTATCTGATCCCACTCTGTATCAGTAAGCTGATTGCCATTGTTGTTCCTTTCAAAGCTAGCAAAGTATATATATGCTCATGCTCACACTATATGATACTAGTTAATATTTGCACTTTTATTCTTTGATGATGTAAAGCTTTAAATTTTATATTTATGGTATACAACTCTAAATCAATATGCTTAAAGGTTGTATAGATATCGAATTCAAGAGAAATAAATTTCATTCTCATGTTAAATTTAGATAGATCACATTTTTCTATTAGTTTTTTTTTGCACATTTTATAACAAGAAGAAGCAATTCTGTCTGATGTTGATGAATGAAATACTAACCGTGAAATAGTAATATACAGGA

Coding sequence (CDS)

ATGATTTTTTTTTCCTTCCTTGAGAAAATGATTGATGCCCTTTTGGAAGGTAAAGGCAGAGCCATAGATTCCTCATCTTGTCTTGTCTCCACTGGATTTTCTTTATGCCAATCTTCGTCAACATTACAAAACTTAGCCAAATTCTCATTCTTTCTCCACTCCGCCATGGAACAACCCACCTCCAATTCTCTTCTACATCTCATTGATTCCCTCAATTCCTTCTTCCCATGGCGCTCCATTGACCAATCCCGCTTCCTCTTCACCTTCTTCGCCGCTATTCTAATTTTCCTCTATATAAAACTCAAGCGCCGCCGCCTGCCACTTCCACCAGGTCCCCGAGGCGTTCCACTCCTCGGAAACCTCCCGTTTCTCGACCCTGAACTTCACACATACTTCACCAAACTGGGTGAAAAATACGGCCCAATAGTAAAGCTTCAACTTGGCAAAAAAATCGGTATAATCGTAAATTCACCGTCAGTAGCACGTGAGGTGTTGAAAGACCAAGATGTCACGTTTGCCAATCGCGATGTTCCTCAAGCTGGGAGGGCCGCCACGTACGGTGGTTCCGACATAGTTTGGACACCGTACGGACCTGAGTGGCGAATGTTGAGAAAAGTTTGCGTGTTAAAGATGTTGAGTAACACCACTTTGGACAGCGTTTACGAACTCCGCTATAGAGAGGTGAGAAACACGGTGGCTCACTTGTACCGGCGATCTGGGTCGGCAGTGAACGTGGGAGAGCAGGGGTTTTTGACGATTTTCAACGTGGTTACAAGTATGTTGTGGGGTGGGTCAGTGGAAGGGGAGCAGAGGGATGGTCTTGCGGCAGAGTTTAGAGAGACGGTTTCGGAAATAACAGAGCTATTGGGAAAGCCTAATGTTTCGGATTTTTTTCCGAGTTTGGCCCGTTTTGATCTCCAAGGGATTGAGAAGCAGATGCGTGAGATTGCTCCAAGATTTGATAACATTTTCGAGAAGATGATTGATCAACGATTGAAAATGGGTAATGAAGACGAGGACGGGAATGTCAAGAAAAATGATTTCTTGCAGTTCTTACTTAAAGTCAAAGATGAAGGAGAATCCAAGACTCCTCTCACCATGACCCACCTTAAAGCCTTGCTCATGGATATGGTGGTCGGCGGGACAGACACATCGTCAAATACCGTAGAATTTGCGATGGCAGAAATGATAAAGAACCCAAAAACTCTAAAGAAAGCACAAGAAGAAATCACCGCCGTAGTCGGAGAAGACAACATCGTGGAAGAATCCCACATTCATAGTTTCCCATATCTCAAAGCCGTAATGAAAGAAACTTTACGTTTACACCCAATTCTACCTCTGCTCGTGCCGCATTGCCCAAGCGAGACCACCATCGTCTCCAATTACGCAATTCCAAAGGGCTCTCGAGTGTTCATCAACGTCTGGGCCATTCAGCGAGACCCCAATCAATGGGAAAATCCATTGGTATTTGAACCAGAGAGATTTCTTGAGACCGACAAGTGGGATTTTGGTGGGAGTGATTTCCGTTATTTCCCATTTGGGTCCGGCAGAAGAAACTGTGCAGGGATAGCCATGGCGGAAAGAATGGTAATTTATTTGCTTGCTACGCTTTTGCATTCTTTTGATTGGAAATTGGAAGATGGTGAGAAGATGGAAGTTGAGGAGAAATTTGGGATTGTTATGAAGATGAAGTCGCCTCTCGTTCTCATTCCAACGCCAAGACTATCTGATCCCACTCTGTATCAGTAA

Protein sequence

MIFFSFLEKMIDALLEGKGRAIDSSSCLVSTGFSLCQSSSTLQNLAKFSFFLHSAMEQPTSNSLLHLIDSLNSFFPWRSIDQSRFLFTFFAAILIFLYIKLKRRRLPLPPGPRGVPLLGNLPFLDPELHTYFTKLGEKYGPIVKLQLGKKIGIIVNSPSVAREVLKDQDVTFANRDVPQAGRAATYGGSDIVWTPYGPEWRMLRKVCVLKMLSNTTLDSVYELRYREVRNTVAHLYRRSGSAVNVGEQGFLTIFNVVTSMLWGGSVEGEQRDGLAAEFRETVSEITELLGKPNVSDFFPSLARFDLQGIEKQMREIAPRFDNIFEKMIDQRLKMGNEDEDGNVKKNDFLQFLLKVKDEGESKTPLTMTHLKALLMDMVVGGTDTSSNTVEFAMAEMIKNPKTLKKAQEEITAVVGEDNIVEESHIHSFPYLKAVMKETLRLHPILPLLVPHCPSETTIVSNYAIPKGSRVFINVWAIQRDPNQWENPLVFEPERFLETDKWDFGGSDFRYFPFGSGRRNCAGIAMAERMVIYLLATLLHSFDWKLEDGEKMEVEEKFGIVMKMKSPLVLIPTPRLSDPTLYQ
Homology
BLAST of Lsi09G003840 vs. ExPASy Swiss-Prot
Match: A0A4D6Q415 (Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B137 PE=1 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 3.7e-100
Identity = 201/509 (39.49%), Postives = 301/509 (59.14%), Query Frame = 0

Query: 85  FLFTFFAAILI--FLYIKLKRRRL----PLPPGPRGVPLLGNLPFLDPELHTYFTKLGEK 144
           F F + + +L+  F+   L RRR     PLPPGP G P+LGNLP L  + H     L ++
Sbjct: 4   FFFLWISTLLLSSFIVYLLYRRRSAQCPPLPPGPNGWPILGNLPQLGAKPHQTLDALSKQ 63

Query: 145 YGPIVKLQLGKKIGIIVNSPSVAREVLKDQDVTFANRDVPQAGRAATYGGSDIVWTPYGP 204
           YGP+ +L+LG    ++ +S +VA + L+  DV F+NR          Y   D+V+ PYGP
Sbjct: 64  YGPLFRLRLGSVNVVVASSSAVAAQFLRTHDVNFSNRPPNSGAEHVAYNYQDLVFAPYGP 123

Query: 205 EWRMLRKVCVLKMLSNTTLDSVYELRYREVRNTVAHLYRR--SGSAVNVGEQGFLTIFNV 264
            WRMLRK+C + + S   LD +  +R  EV   V +L R   +G  VN+G+   +   N 
Sbjct: 124 RWRMLRKLCSVHLFSLKALDDLRPVRQGEVACLVRNLRRHADTGVLVNLGKALNVCATNA 183

Query: 265 VTSMLWGGSVEGEQRDGLAA--EFRETVSEITELLGKPNVSDFFPSLARFDLQGIEKQMR 324
           +   + G  V  ++   LA   EF+E V E+  L G  NV DF P L   DLQG+  +M+
Sbjct: 184 LARAMLGRRVFADEDAQLAEADEFKEMVVELMRLAGVFNVGDFVPGLGWLDLQGVVGKMK 243

Query: 325 EIAPRFDNIFEKMIDQRLKMGNEDEDGNVKKNDFLQFLLKVKD-EGESKTPLTMTHLKAL 384
            +  R+D   +++I        E+   N K  D L  L+++K+ + E +  L  T +KAL
Sbjct: 244 RLHRRYDAFLDRVI--------EENQANAKSGDLLSVLIRLKEADAEGEIKLNNTDIKAL 303

Query: 385 LMDMVVGGTDTSSNTVEFAMAEMIKNPKTLKKAQEEITAVVGEDNIVEESHIHSFPYLKA 444
           L+++   GTDTSS+TVE+ +AE+I++P  L+K Q E+ +V+G D +V ES + + PYL+A
Sbjct: 304 LLNLFTAGTDTSSSTVEWVLAELIRHPDILQKTQHELDSVIGRDRLVAESDLPNLPYLQA 363

Query: 445 VMKETLRLHPILPLLVPHCPSETTIVSNYAIPKGSRVFINVWAIQRDPNQWENPLVFEPE 504
           V+KET RLHP  PL +P   SE  IV+ Y IPK + + +NVW+I RD   W +PL F P 
Sbjct: 364 VVKETFRLHPSTPLSLPRMASEECIVNGYKIPKHATLLVNVWSIGRDAAVWNDPLEFRPS 423

Query: 505 RFL---ETDKWDFGGSDFRYFPFGSGRRNCAGIAMAERMVIYLLATLLHSFDWKLEDG-- 564
           RFL   E +  D  G+DF   PFG+GRR CAG+++  RMV ++ AT++H++DW L  G  
Sbjct: 424 RFLPGGEREHVDVKGNDFEVIPFGAGRRICAGLSLGLRMVQFMTATIVHAYDWSLPKGQE 483

Query: 565 -EKMEVEEKFGIVMKMKSPLVLIPTPRLS 577
            +K+++EE +G+ ++   PL++ P PRLS
Sbjct: 484 CQKLDMEEAYGLTLQRAVPLMVQPIPRLS 504

BLAST of Lsi09G003840 vs. ExPASy Swiss-Prot
Match: Q8VWZ7 (Geraniol 8-hydroxylase OS=Catharanthus roseus OX=4058 GN=CYP76B6 PE=1 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 1.6e-98
Identity = 197/496 (39.72%), Postives = 294/496 (59.27%), Query Frame = 0

Query: 86  LFTFFAAILIFLYIKLKRRRLPLPPGPRGVPLLGNLPFLDPELHTYFTKLGEKYGPIVKL 145
           L   FA  L   +  L RR   LPPGP  +P +G+L  L  + H    KL +K+GPI+ L
Sbjct: 8   LTLLFALTLYEAFSYLSRRTKNLPPGPSPLPFIGSLHLLGDQPHKSLAKLSKKHGPIMSL 67

Query: 146 QLGKKIGIIVNSPSVAREVLKDQDVTFANRDVPQAGRAATYGGSDIVWTPYGPEWRMLRK 205
           +LG+   I+++S ++A+EVL+ QD+ F++R VP A  A       +VW P    WR LRK
Sbjct: 68  KLGQITTIVISSSTMAKEVLQKQDLAFSSRSVPNALHAHNQFKFSVVWLPVASRWRSLRK 127

Query: 206 VCVLKMLSNTTLDSVYELRYREVRNTVAHLYR--RSGSAVNVGEQGFLTIFNVVTSMLWG 265
           V    + S   LD+   LR R+V+  +A+  +  +SG AV+VG   F T  N+++++++ 
Sbjct: 128 VLNSNIFSGNRLDANQHLRTRKVQELIAYCRKNSQSGEAVDVGRAAFRTSLNLLSNLIFS 187

Query: 266 GSVEGEQRDGLAAEFRETVSEITELLGKPNVSDFFPSLARFDLQGIEKQMR----EIAPR 325
             +     D  A EF++ V  I    GKPN+ DFFP L + D QGI  +M     E+   
Sbjct: 188 KDLTDPYSDS-AKEFKDLVWNIMVEAGKPNLVDFFPLLEKVDPQGIRHRMTIHFGEVLKL 247

Query: 326 FDNIFEKMIDQRLKMGNEDEDGNVKKNDFLQFLLKVKDEGESKTPLTMTHLKALLMDMVV 385
           F  +  + ++QR   G        +KND L  LL      ES   +  TH++ + +D+ V
Sbjct: 248 FGGLVNERLEQRRSKG--------EKNDVLDVLLTTSQ--ESPEEIDRTHIERMCLDLFV 307

Query: 386 GGTDTSSNTVEFAMAEMIKNPKTLKKAQEEITAVVGEDNIVEESHIHSFPYLKAVMKETL 445
            GTDT+S+T+E+AM+EM+KNP  +KK Q+E+  V+G    +EES I+  PYL+ VMKETL
Sbjct: 308 AGTDTTSSTLEWAMSEMLKNPDKMKKTQDELAQVIGRGKTIEESDINRLPYLRCVMKETL 367

Query: 446 RLHPILPLLVPHCPSETTIVSNYAIPKGSRVFINVWAIQRDPNQWENPLVFEPERFLETD 505
           R+HP +P L+P    ++  V  Y +PKGS+V +N WAI RD   W++ L F+PERF+E++
Sbjct: 368 RIHPPVPFLIPRKVEQSVEVCGYNVPKGSQVLVNAWAIGRDETVWDDALAFKPERFMESE 427

Query: 506 KWDFGGSDFRYFPFGSGRRNCAGIAMAERMVIYLLATLLHSFDWKLEDG---EKMEVEEK 565
             D  G DF   PFG+GRR C G+ +A R V  +L +LL+SF+WKLE G   + +++EEK
Sbjct: 428 -LDIRGRDFELIPFGAGRRICPGLPLALRTVPLMLGSLLNSFNWKLEGGMAPKDLDMEEK 487

Query: 566 FGIVMKMKSPLVLIPT 573
           FGI ++   PL  +P+
Sbjct: 488 FGITLQKAHPLRAVPS 491

BLAST of Lsi09G003840 vs. ExPASy Swiss-Prot
Match: D1MI46 (Geraniol 8-hydroxylase OS=Swertia mussotii OX=137888 GN=CYP76B10 PE=1 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 3.9e-97
Identity = 195/494 (39.47%), Postives = 292/494 (59.11%), Query Frame = 0

Query: 85  FLFTFFAAILIFLYIKLKRRRLPLPPGPRGVPLLGNLPFLDPELHTYFTKLGEKYGPIVK 144
           F  T + A+  F      R+   LPPGP  +PL+GNL  L  + H    KL +K+GPI+ 
Sbjct: 14  FTITLYQALNFF-----SRKSKNLPPGPSPLPLIGNLHLLGDQPHKSLAKLAKKHGPIMG 73

Query: 145 LQLGKKIGIIVNSPSVAREVLKDQDVTFANRDVPQAGRAATYGGSDIVWTPYGPEWRMLR 204
           LQLG+   I+V S  +A+EVL+ QD+ F++R +P A  A       ++W P    WR LR
Sbjct: 74  LQLGQVTTIVVTSSGMAKEVLQKQDLAFSSRSIPNAIHAHDQYKYSVIWLPVASRWRGLR 133

Query: 205 KVCVLKMLSNTTLDSVYELRYREVRNTVAHLYRRS--GSAVNVGEQGFLTIFNVVTSMLW 264
           K     M S   LD+   LR R+V+  +A+  + S  G A++VG   F T  N++++ ++
Sbjct: 134 KALNSNMFSGNRLDANQHLRSRKVQELIAYCRKSSQTGDAIDVGRAAFRTSLNLLSNTMF 193

Query: 265 GGSVEGEQRDGLAAEFRETVSEITELLGKPNVSDFFPSLARFDLQGIEKQMREIAPRFDN 324
              +     D  A EF++ V  +    GKPN+ D+FP L + D QGI K+M     +   
Sbjct: 194 SKDLTDPYSDS-AKEFKDLVWNVMVEAGKPNLVDYFPLLDKVDPQGIRKRMTIHFGKILE 253

Query: 325 IFEKMIDQRLKMGNEDEDGNVKKNDFLQFLLKVKDEGESKTPLTMTHLKALLMDMVVGGT 384
           +F  +ID+RL    + +      +D L  LL   +  ES   +  TH++ + +D+ V GT
Sbjct: 254 LFGGLIDERL----QQKKAKGVNDDVLDVLLTTSE--ESPEEIDRTHIQRMCLDLFVAGT 313

Query: 385 DTSSNTVEFAMAEMIKNPKTLKKAQEEITAVVGEDNIVEESHIHSFPYLKAVMKETLRLH 444
           DT+S+T+E+AM+EM+KNP+ +K AQ E+  V+G+   VEE+ +   PYL+  +KETLR+H
Sbjct: 314 DTTSSTLEWAMSEMLKNPEKMKAAQAELAQVIGKGKAVEEADLARLPYLRCAIKETLRIH 373

Query: 445 PILPLLVPHCPSETTIVSNYAIPKGSRVFINVWAIQRDPNQWENPLVFEPERFLETDKWD 504
           P +PLL+P    +   V  Y +PK S+V +NVWAI RD   W++PL F+PERFLE++  +
Sbjct: 374 PPVPLLIPRRTEQEVEVCGYTVPKNSQVLVNVWAISRDDAIWKDPLSFKPERFLESE-LE 433

Query: 505 FGGSDFRYFPFGSGRRNCAGIAMAERMVIYLLATLLHSFDWKLEDG---EKMEVEEKFGI 564
             G DF   PFG+GRR C G+ +A RMV  +L +LL+SFDWKLE G   + +++EEKFGI
Sbjct: 434 MRGKDFELIPFGAGRRICPGLPLAVRMVPVMLGSLLNSFDWKLEGGIAPKDLDMEEKFGI 493

Query: 565 VMKMKSPLVLIPTP 574
            ++   PL  + TP
Sbjct: 494 TLQKAHPLRAVATP 494

BLAST of Lsi09G003840 vs. ExPASy Swiss-Prot
Match: Q9SD85 (Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP75B1 PE=1 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 6.6e-97
Identity = 198/500 (39.60%), Postives = 286/500 (57.20%), Query Frame = 0

Query: 90  FAAILIFLYIKLKRRRLPLPPGPRGVPLLGNLPFLDPELHTYFTKLGEKYGPIVKLQLGK 149
           F  + IF + + +     LPPGP   P++GNLP +  + H   + +   YGPI+ L+LG 
Sbjct: 15  FLILRIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKPHRTLSAMVTTYGPILHLRLGF 74

Query: 150 KIGIIVNSPSVAREVLKDQDVTFANRDVPQAGRAATYGGSDIVWTPYGPEWRMLRKVCVL 209
              ++  S SVA + LK  D  FA+R      +   Y   D+V+ PYG  WR+LRK+  +
Sbjct: 75  VDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPYGHRWRLLRKISSV 134

Query: 210 KMLSNTTLDSVYELRYREVRNTVAHLYRRSGSAVNVGEQGFLTIFNVVTSMLWGGSVEGE 269
            + S   L+    +R  EV      L R     VN+G+   + + N +   + G  + G 
Sbjct: 135 HLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVVNALGREMIGRRLFGA 194

Query: 270 QRDGLAAEFRETVSEITELLGKPNVSDFFPSLARFDLQGIEKQMREIAPRFDNIFEKMID 329
             D  A EFR  V+E+  L G  N+ DF PSL   DLQG+  +M+ +  RFD     ++ 
Sbjct: 195 DADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKMKRLHKRFDAFLSSILK 254

Query: 330 QRLKMGNEDEDGNVKKNDFLQFLLKVK--DEGESKTPLTMTHLKALLMDMVVGGTDTSSN 389
           +  +M  +D+    K  D L  L+ +K  D       LT T +KALL++M   GTDTS++
Sbjct: 255 EH-EMNGQDQ----KHTDMLSTLISLKGTDLDGDGGSLTDTEIKALLLNMFTAGTDTSAS 314

Query: 390 TVEFAMAEMIKNPKTLKKAQEEITAVVGEDNIVEESHIHSFPYLKAVMKETLRLHPILPL 449
           TV++A+AE+I++P  + KAQEE+  VVG D  V ES I   PYL+AV+KE  RLHP  PL
Sbjct: 315 TVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQAVIKENFRLHPPTPL 374

Query: 450 LVPHCPSETTIVSNYAIPKGSRVFINVWAIQRDPNQWENPLVFEPERFL---ETDKWDFG 509
            +PH  SE+  ++ Y IPKGS +  N+WAI RDP+QW +PL F+PERFL   E    D  
Sbjct: 375 SLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKPERFLPGGEKSGVDVK 434

Query: 510 GSDFRYFPFGSGRRNCAGIAMAERMVIYLLATLLHSFDWKLEDG---EKMEVEEKFGIVM 569
           GSDF   PFG+GRR CAG+++  R + +L ATL+  FDW+L  G   EK+ +EE +G+ +
Sbjct: 435 GSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGVTPEKLNMEESYGLTL 494

Query: 570 KMKSPLVLIPTPRLSDPTLY 582
           +   PLV+ P PRL+ P +Y
Sbjct: 495 QRAVPLVVHPKPRLA-PNVY 508

BLAST of Lsi09G003840 vs. ExPASy Swiss-Prot
Match: Q9SBQ9 (Flavonoid 3'-monooxygenase OS=Petunia hybrida OX=4102 GN=CYP75B2 PE=2 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 5.6e-96
Identity = 196/503 (38.97%), Postives = 287/503 (57.06%), Query Frame = 0

Query: 86  LFTFFAAILIFLYIKLKRRRLPLPPGPRGVPLLGNLPFLDPELHTYFTKLGEKYGPIVKL 145
           +F+F    ++  + + KR  LPLPPGP+  P++GNL  L P+ H     + + YGP++ L
Sbjct: 12  IFSFLLQFILRSFFR-KRYPLPLPPGPKPWPIIGNLVHLGPKPHQSTAAMAQTYGPLMYL 71

Query: 146 QLGKKIGIIVNSPSVAREVLKDQDVTFANRDVPQAGRAATYGGSDIVWTPYGPEWRMLRK 205
           ++G    ++  S SVA + LK  D  F++R          Y   D+V+ PYGP WRMLRK
Sbjct: 72  KMGFVDVVVAASASVAAQFLKTHDANFSSRPPNSGAEHMAYNYQDLVFAPYGPRWRMLRK 131

Query: 206 VCVLKMLSNTTLDSVYELRYREVRNTVAHLYRRSGSAVNVGEQGFLTIFNVVTSMLWGGS 265
           +C + + S   LD    +R  EV+     L       V +G+   +   N +  ++ G  
Sbjct: 132 ICSVHLFSTKALDDFRHVRQDEVKTLTRALASAGQKPVKLGQLLNVCTTNALARVMLGKR 191

Query: 266 VEGE---QRDGLAAEFRETVSEITELLGKPNVSDFFPSLARFDLQGIEKQMREIAPRFDN 325
           V  +     D  AAEF+  V E+  + G  N+ DF P L   D+QG+  +M+++  RFD 
Sbjct: 192 VFADGSGDVDPQAAEFKSMVVEMMVVAGVFNIGDFIPQLNWLDIQGVAAKMKKLHARFDA 251

Query: 326 IFEKMIDQRLKMGNEDEDGNV--KKNDFLQFLLKVK--DEGESKTPLTMTHLKALLMDMV 385
               ++        E+  G +  +  D L  L+ +K  D       LT T +KALL+++ 
Sbjct: 252 FLTDIL--------EEHKGKIFGEMKDLLSTLISLKNDDADNDGGKLTDTEIKALLLNLF 311

Query: 386 VGGTDTSSNTVEFAMAEMIKNPKTLKKAQEEITAVVGEDNIVEESHIHSFPYLKAVMKET 445
           V GTDTSS+TVE+A+AE+I+NPK L +AQ+EI  VVG D +V E  +    YL+A++KET
Sbjct: 312 VAGTDTSSSTVEWAIAELIRNPKILAQAQQEIDKVVGRDRLVGELDLAQLTYLEAIVKET 371

Query: 446 LRLHPILPLLVPHCPSETTIVSNYAIPKGSRVFINVWAIQRDPNQWENPLVFEPERFL-- 505
            RLHP  PL +P   SE+  ++ Y IPKGS + +NVWAI RDPN W +PL F PERFL  
Sbjct: 372 FRLHPSTPLSLPRIASESCEINGYFIPKGSTLLLNVWAIARDPNAWADPLEFRPERFLPG 431

Query: 506 -ETDKWDFGGSDFRYFPFGSGRRNCAGIAMAERMVIYLLATLLHSFDWKLEDG---EKME 565
            E  K D  G+DF   PFG+GRR CAG+ +  RMV  ++ATL+H+F+W L  G   E + 
Sbjct: 432 GEKPKVDVRGNDFEVIPFGAGRRICAGMNLGIRMVQLMIATLIHAFNWDLVSGQLPEMLN 491

Query: 566 VEEKFGIVMKMKSPLVLIPTPRL 576
           +EE +G+ ++   PLV+ P PRL
Sbjct: 492 MEEAYGLTLQRADPLVVHPRPRL 505

BLAST of Lsi09G003840 vs. ExPASy TrEMBL
Match: A0A0A0KR55 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G159600 PE=3 SV=1)

HSP 1 Score: 894.0 bits (2309), Expect = 3.2e-256
Identity = 443/528 (83.90%), Postives = 482/528 (91.29%), Query Frame = 0

Query: 56  MEQPTSNSLLHLIDSLNSFFPWRSIDQSRFLFTFFAAILIFLYIKLKRRRLPLPPGPRGV 115
           ME PTSN           FF   S DQS F FTF AA+LIFLY++L R R+PLPPGPRGV
Sbjct: 1   MENPTSN-----------FF---SFDQSHFFFTFLAALLIFLYLRLTRLRVPLPPGPRGV 60

Query: 116 PLLGNLPFLDPELHTYFTKLGEKYGPIVKLQLGKKIGIIVNSPSVAREVLKDQDVTFANR 175
           PLLGNLPFL PELHTYF +LG+KYGPIVKLQLG+KIGII+NSPSV REVLKD DVTFANR
Sbjct: 61  PLLGNLPFLHPELHTYFAQLGQKYGPIVKLQLGRKIGIIINSPSVVREVLKDHDVTFANR 120

Query: 176 DVPQAGRAATYGGSDIVWTPYGPEWRMLRKVCVLKMLSNTTLDSVYELRYREVRNTVAHL 235
           DVPQAGRAA+YGGSDIVWTPYGP+WRMLRKVCV+KMLSN TLDSVYELR +EVRNTVAHL
Sbjct: 121 DVPQAGRAASYGGSDIVWTPYGPKWRMLRKVCVVKMLSNATLDSVYELRRKEVRNTVAHL 180

Query: 236 YRRSGSAVNVGEQGFLTIFNVVTSMLWGGSVEGEQRDGLAAEFRETVSEITELLGKPNVS 295
           Y R+G+AVNVGEQGFLT+FNVVTSMLWGGSVEGEQRDGLAAEFRETVSE+TELLG PNVS
Sbjct: 181 YARAGTAVNVGEQGFLTVFNVVTSMLWGGSVEGEQRDGLAAEFRETVSEMTELLGLPNVS 240

Query: 296 DFFPSLARFDLQGIEKQMREIAPRFDNIFEKMIDQRLKMGNEDEDGNVKKNDFLQFLLKV 355
           DFFPSLARFDLQGIEK+MRE+APRFD+IFEKMIDQRLK+   DE G+VKKNDFLQFLL+V
Sbjct: 241 DFFPSLARFDLQGIEKKMRELAPRFDSIFEKMIDQRLKIDGGDEGGSVKKNDFLQFLLQV 300

Query: 356 KDEGESKTPLTMTHLKALLMDMVVGGTDTSSNTVEFAMAEMIKNPKTLKKAQEEITAVVG 415
           KDE ESKTPLTMTHLKALLMDMV+GGTDTSSNTVEFAMAEM+K+PKTLKKAQEE+ AVVG
Sbjct: 301 KDEEESKTPLTMTHLKALLMDMVIGGTDTSSNTVEFAMAEMLKSPKTLKKAQEEVVAVVG 360

Query: 416 EDNIVEESHIHSFPYLKAVMKETLRLHPILPLLVPHCPSETTIVSNYAIPKGSRVFINVW 475
           EDNIVEESHIHS PYLKAVMKETLRLHPILPLLVPHCPSET IVSNY IPKGSRVFINVW
Sbjct: 361 EDNIVEESHIHSLPYLKAVMKETLRLHPILPLLVPHCPSETAIVSNYTIPKGSRVFINVW 420

Query: 476 AIQRDPNQWENPLVFEPERFLETDKWDFGGSDFRYFPFGSGRRNCAGIAMAERMVIYLLA 535
           AIQRDP  W+NPL F+PERFL   K+DF G+DFRYFPFGSGRRNCAGIAMAERMV+Y+LA
Sbjct: 421 AIQRDPKNWDNPLEFDPERFL-NGKFDFSGNDFRYFPFGSGRRNCAGIAMAERMVMYMLA 480

Query: 536 TLLHSFDWKLEDGE-KMEVEEKFGIVMKMKSPLVLIPTPRLSDPTLYQ 583
           TLLHSFDWKLE+G+ K+EVEEKFGIV+KMK+PLVLIPTP+L+DPTLYQ
Sbjct: 481 TLLHSFDWKLEEGDGKIEVEEKFGIVLKMKTPLVLIPTPKLADPTLYQ 513

BLAST of Lsi09G003840 vs. ExPASy TrEMBL
Match: A0A1S3ASY0 (flavonoid 3'-monooxygenase-like OS=Cucumis melo OX=3656 GN=LOC103482651 PE=3 SV=1)

HSP 1 Score: 892.1 bits (2304), Expect = 1.2e-255
Identity = 442/528 (83.71%), Postives = 480/528 (90.91%), Query Frame = 0

Query: 56  MEQPTSNSLLHLIDSLNSFFPWRSIDQSRFLFTFFAAILIFLYIKLKRRRLPLPPGPRGV 115
           ME PTSN           FF   SIDQS F FTF AA+LIFLY++L R R+PLPPGPRGV
Sbjct: 1   MEDPTSN-----------FF---SIDQSHFFFTFLAALLIFLYLRLTRLRVPLPPGPRGV 60

Query: 116 PLLGNLPFLDPELHTYFTKLGEKYGPIVKLQLGKKIGIIVNSPSVAREVLKDQDVTFANR 175
           PLLGNLPFLDPELHTYF +L +KYGPIVKLQLG+KIGII+NSPSV REVLKD DVTFANR
Sbjct: 61  PLLGNLPFLDPELHTYFAQLSQKYGPIVKLQLGRKIGIIINSPSVVREVLKDHDVTFANR 120

Query: 176 DVPQAGRAATYGGSDIVWTPYGPEWRMLRKVCVLKMLSNTTLDSVYELRYREVRNTVAHL 235
           DVP AGRAA+YGGSDIVWTPYGP+WRMLRKVCVLKMLSN TLDSVYELR REVRNTVAHL
Sbjct: 121 DVPHAGRAASYGGSDIVWTPYGPQWRMLRKVCVLKMLSNATLDSVYELRRREVRNTVAHL 180

Query: 236 YRRSGSAVNVGEQGFLTIFNVVTSMLWGGSVEGEQRDGLAAEFRETVSEITELLGKPNVS 295
           Y R+G+ VNVGEQGFLT+FNVVTSMLWGGSVEGEQRDGLAAEFRETVSE+TELLG PNVS
Sbjct: 181 YARAGTRVNVGEQGFLTVFNVVTSMLWGGSVEGEQRDGLAAEFRETVSEMTELLGLPNVS 240

Query: 296 DFFPSLARFDLQGIEKQMREIAPRFDNIFEKMIDQRLKMGNEDEDGNVKKNDFLQFLLKV 355
           DFFPSLARFD+QGIEK+MRE+APRFD+IFEKMIDQRLK+  +DE  +VKKNDFLQFLL+V
Sbjct: 241 DFFPSLARFDIQGIEKKMRELAPRFDSIFEKMIDQRLKIDGKDEGESVKKNDFLQFLLQV 300

Query: 356 KDEGESKTPLTMTHLKALLMDMVVGGTDTSSNTVEFAMAEMIKNPKTLKKAQEEITAVVG 415
           KD+GESKTPLTMTHLKALLMDMV+GGTDTSSNTVEFAMAEM+K+PKTLKKAQ+E+ AVVG
Sbjct: 301 KDDGESKTPLTMTHLKALLMDMVIGGTDTSSNTVEFAMAEMLKSPKTLKKAQQELVAVVG 360

Query: 416 EDNIVEESHIHSFPYLKAVMKETLRLHPILPLLVPHCPSETTIVSNYAIPKGSRVFINVW 475
           EDNIVEESHIHS PYLKAVMKETLRLHPILPLLVPHCPSET IVSNY IPKGSRVFINVW
Sbjct: 361 EDNIVEESHIHSLPYLKAVMKETLRLHPILPLLVPHCPSETAIVSNYTIPKGSRVFINVW 420

Query: 476 AIQRDPNQWENPLVFEPERFLETDKWDFGGSDFRYFPFGSGRRNCAGIAMAERMVIYLLA 535
           AIQRDP  W+NPL F+PERFL   K+DF G+DFRYFPFGSGRRNCAGI MAERMV+YLLA
Sbjct: 421 AIQRDPKNWDNPLEFDPERFL-NGKYDFSGNDFRYFPFGSGRRNCAGIGMAERMVMYLLA 480

Query: 536 TLLHSFDWKLEDG-EKMEVEEKFGIVMKMKSPLVLIPTPRLSDPTLYQ 583
           TLLHSFDWKL DG EK+EVEEKFGIV+KMK+PLV+IPTP+LSDPTLYQ
Sbjct: 481 TLLHSFDWKLGDGDEKIEVEEKFGIVLKMKTPLVVIPTPKLSDPTLYQ 513

BLAST of Lsi09G003840 vs. ExPASy TrEMBL
Match: A0A6J1E5N3 (geraniol 8-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111430178 PE=3 SV=1)

HSP 1 Score: 872.5 bits (2253), Expect = 1.0e-249
Identity = 427/529 (80.72%), Postives = 473/529 (89.41%), Query Frame = 0

Query: 58  QPTSNSLLHLIDSLNSFFPWRSID----QSRFLFT-FFAAILIFLYIKLKRRRLPLPPGP 117
           + TSNSL  LIDSLN   PWR ID    QSR LFT FFA +LIFLY K+ RRR+PLPPGP
Sbjct: 2   ETTSNSLQTLIDSLNFLLPWRWIDAADNQSRLLFTVFFAGLLIFLYTKVTRRRVPLPPGP 61

Query: 118 RGVPLLGNLPFLDPELHTYFTKLGEKYGPIVKLQLGKKIGIIVNSPSVAREVLKDQDVTF 177
            GVPLLGNLPFLDPELHTYF  LG+KYGPIVKLQLG K+GIIVNSPSVARE+LKD DVTF
Sbjct: 62  LGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDHDVTF 121

Query: 178 ANRDVPQAGRAATYGGSDIVWTPYGPEWRMLRKVCVLKMLSNTTLDSVYELRYREVRNTV 237
           ANRDVPQAGRAATYGGS+IVW PYG EWRMLRKVCV+KMLSN TL+SVY+LR REVRNTV
Sbjct: 122 ANRDVPQAGRAATYGGSNIVWNPYGSEWRMLRKVCVVKMLSNATLESVYDLRRREVRNTV 181

Query: 238 AHLYRRSGSAVNVGEQGFLTIFNVVTSMLWGGSVEGEQRDGLAAEFRETVSEITELLGKP 297
           A+LY R+GSAVNVGEQGFLT+FNVVT MLWGG +EG+QRD LAAEFRETVS+ TELLGKP
Sbjct: 182 ANLYGRAGSAVNVGEQGFLTVFNVVTGMLWGGIMEGDQRDSLAAEFRETVSDFTELLGKP 241

Query: 298 NVSDFFPSLARFDLQGIEKQMREIAPRFDNIFEKMIDQRLKMGNEDEDGNVKKNDFLQFL 357
           NVSDFFPSLARFDLQGIEK+MR++A +FD IFE MI+QRLK+   ++  + + +DFLQFL
Sbjct: 242 NVSDFFPSLARFDLQGIEKKMRKLALKFDTIFENMINQRLKIVGGEDGQSAQNHDFLQFL 301

Query: 358 LKVKDEGESKTPLTMTHLKALLMDMVVGGTDTSSNTVEFAMAEMIKNPKTLKKAQEEITA 417
           L++KD G+SKTPLTM HLKALLMDMV+GGTDTSSNTVEFA+AEMI NP TLKKAQEE+ A
Sbjct: 302 LEMKDAGDSKTPLTMIHLKALLMDMVIGGTDTSSNTVEFALAEMINNPATLKKAQEEVAA 361

Query: 418 VVGEDNIVEESHIHSFPYLKAVMKETLRLHPILPLLVPHCPSETTIVSNYAIPKGSRVFI 477
           VVGEDN+VEESHIHS PYLKAVMKETLRLHPILPLLVPH PSETT +S Y IPKGSRVFI
Sbjct: 362 VVGEDNMVEESHIHSLPYLKAVMKETLRLHPILPLLVPHSPSETTTISKYTIPKGSRVFI 421

Query: 478 NVWAIQRDPNQWENPLVFEPERFLETDKWDFGGSDFRYFPFGSGRRNCAGIAMAERMVIY 537
           NVWAIQRDPNQWENPLVF+PERFL+   WDFGGSDFRYFPFGSGRRNCAGI MAER  +Y
Sbjct: 422 NVWAIQRDPNQWENPLVFDPERFLDNQTWDFGGSDFRYFPFGSGRRNCAGIGMAERTFMY 481

Query: 538 LLATLLHSFDWKLEDGEKMEVEEKFGIVMKMKSPLVLIPTPRLSDPTLY 582
           LLATLLHSFDWK+E+G+K+E+EEKFGIV+KMK+PLVLIPTPRLSDPTLY
Sbjct: 482 LLATLLHSFDWKVEEGKKLEIEEKFGIVLKMKTPLVLIPTPRLSDPTLY 530

BLAST of Lsi09G003840 vs. ExPASy TrEMBL
Match: A0A6J1I001 (geraniol 8-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111469168 PE=3 SV=1)

HSP 1 Score: 871.7 bits (2251), Expect = 1.7e-249
Identity = 427/530 (80.57%), Postives = 470/530 (88.68%), Query Frame = 0

Query: 58  QPTSNSLLHLIDSLNSFFPWRSID----QSRFLFT-FFAAILIFLYIKLKRRRLPLPPGP 117
           +PTSN L   IDSLN   PWR ID    QSR LFT FFA +LIFLY K+ RRR+PLPPGP
Sbjct: 2   EPTSNPLQIFIDSLNFLLPWRWIDAADNQSRLLFTVFFAGLLIFLYTKVTRRRVPLPPGP 61

Query: 118 RGVPLLGNLPFLDPELHTYFTKLGEKYGPIVKLQLGKKIGIIVNSPSVAREVLKDQDVTF 177
            GVPLLGNLPFLDPELHTYF  LG+KYGPIVKLQLG K+GIIVNSPSVARE+LKD DVTF
Sbjct: 62  LGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDNDVTF 121

Query: 178 ANRDVPQAGRAATYGGSDIVWTPYGPEWRMLRKVCVLKMLSNTTLDSVYELRYREVRNTV 237
           ANRDVPQAGRAA+YGGSDI WTPYG EWRMLRKVCVLKMLSN TL+SVY+LR REVRNTV
Sbjct: 122 ANRDVPQAGRAASYGGSDIAWTPYGAEWRMLRKVCVLKMLSNATLESVYDLRRREVRNTV 181

Query: 238 AHLYRRSGSAVNVGEQGFLTIFNVVTSMLWGGSVEGEQRDGLAAEFRETVSEITELLGKP 297
           A LY R+GSAVNVGEQ FLTIFNVVTSMLWGG+VEG+QRD LAAEFRETVS++TELLGKP
Sbjct: 182 AILYGRAGSAVNVGEQSFLTIFNVVTSMLWGGTVEGDQRDSLAAEFRETVSDLTELLGKP 241

Query: 298 NVSDFFPSLARFDLQGIEKQMREIAPRFDNIFEKMIDQRLKMGNEDEDGNVKKNDFLQFL 357
           NVSDFFP LARFDLQGIEK+M ++A +FD IFE MI+QRLK+   ++  + K  DFLQFL
Sbjct: 242 NVSDFFPCLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKISGGEDGESAKNKDFLQFL 301

Query: 358 LKVKDEGESKTPLTMTHLKALLMDMVVGGTDTSSNTVEFAMAEMIKNPKTLKKAQEEITA 417
           L+VKD G+SKTPLT+THLKALLMDMV GGTDT+SNT+EFA+AEMI NP TLKKAQEE+ A
Sbjct: 302 LEVKDSGDSKTPLTITHLKALLMDMVTGGTDTTSNTIEFALAEMINNPATLKKAQEEVAA 361

Query: 418 VVGEDNIVEESHIHSFPYLKAVMKETLRLHPILPLLVPHCPSETTIVSNYAIPKGSRVFI 477
           VVGEDN+VEE HIHS PYLKAVMKETLRLHPILPLLVPH PSETT +S Y IPKGSRVFI
Sbjct: 362 VVGEDNMVEECHIHSLPYLKAVMKETLRLHPILPLLVPHSPSETTTISKYTIPKGSRVFI 421

Query: 478 NVWAIQRDPNQWENPLVFEPERFLETDKWDFGGSDFRYFPFGSGRRNCAGIAMAERMVIY 537
           NVWAIQR PNQWENPLVF+PERFL   KWDFGGSDFRYFPFGSGRRNCAGIAMAER  +Y
Sbjct: 422 NVWAIQRHPNQWENPLVFDPERFLNNQKWDFGGSDFRYFPFGSGRRNCAGIAMAERTFMY 481

Query: 538 LLATLLHSFDWKLEDGEKMEVEEKFGIVMKMKSPLVLIPTPRLSDPTLYQ 583
           LLATLLHSF+WK+E+G+K+E+EEKFGIV+KMK+PLVLIPTPRLSDPTLYQ
Sbjct: 482 LLATLLHSFNWKVEEGKKLEIEEKFGIVLKMKTPLVLIPTPRLSDPTLYQ 531

BLAST of Lsi09G003840 vs. ExPASy TrEMBL
Match: A0A6J1HYH5 (geraniol 8-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111469169 PE=3 SV=1)

HSP 1 Score: 855.5 bits (2209), Expect = 1.3e-244
Identity = 421/529 (79.58%), Postives = 465/529 (87.90%), Query Frame = 0

Query: 58  QPTSNSLLHLIDSLNSFFPWRSID----QSRFLFT-FFAAILIFLYIKLKRRRLPLPPGP 117
           + TSNSL  LIDSLN   PW  ID    Q RFLFT FFA +LIFLY K+ RRR+PLPPGP
Sbjct: 2   ETTSNSLRTLIDSLNFLLPWPWIDTADYQFRFLFTVFFAGLLIFLYTKVTRRRVPLPPGP 61

Query: 118 RGVPLLGNLPFLDPELHTYFTKLGEKYGPIVKLQLGKKIGIIVNSPSVAREVLKDQDVTF 177
            GVPLLGNL FLDPELHTYF  LG+KYGPIVKLQLG K+GIIVNSPSVARE+LKD DVTF
Sbjct: 62  LGVPLLGNLLFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDNDVTF 121

Query: 178 ANRDVPQAGRAATYGGSDIVWTPYGPEWRMLRKVCVLKMLSNTTLDSVYELRYREVRNTV 237
           ANRDVPQAGRAA YGGS+IVW PYG EWRMLRKVCVLKMLSN TL+SVY+LR REVRNTV
Sbjct: 122 ANRDVPQAGRAARYGGSNIVWNPYGAEWRMLRKVCVLKMLSNATLESVYDLRRREVRNTV 181

Query: 238 AHLYRRSGSAVNVGEQGFLTIFNVVTSMLWGGSVEGEQRDGLAAEFRETVSEITELLGKP 297
           A LY R+GSAVNVGEQ FLTIFNVVTSMLWGG+VEG+QRD LAAEFRETVS++TELLGKP
Sbjct: 182 AILYGRAGSAVNVGEQSFLTIFNVVTSMLWGGTVEGDQRDSLAAEFRETVSDLTELLGKP 241

Query: 298 NVSDFFPSLARFDLQGIEKQMREIAPRFDNIFEKMIDQRLKMGNEDEDGNVKKNDFLQFL 357
           NVSDFFP LARFDLQGIEK+M ++A +FD IFE MI+QRLK+   ++  + K  DFLQFL
Sbjct: 242 NVSDFFPCLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKIAGGEDGESAKNKDFLQFL 301

Query: 358 LKVKDEGESKTPLTMTHLKALLMDMVVGGTDTSSNTVEFAMAEMIKNPKTLKKAQEEITA 417
           L+VKD G+SKTPLT+THLKALLMDMV+GGTDT+SNT+EFA+AEMI NP TLKKAQEE+ A
Sbjct: 302 LEVKDSGDSKTPLTITHLKALLMDMVIGGTDTTSNTIEFALAEMINNPATLKKAQEEVAA 361

Query: 418 VVGEDNIVEESHIHSFPYLKAVMKETLRLHPILPLLVPHCPSETTIVSNYAIPKGSRVFI 477
           VVGEDN+VEE HIHS  YLKAVMKETLRLHPILPLLVPH PSETT +S Y IPKGSRVFI
Sbjct: 362 VVGEDNMVEECHIHSLSYLKAVMKETLRLHPILPLLVPHSPSETTTISKYTIPKGSRVFI 421

Query: 478 NVWAIQRDPNQWENPLVFEPERFLETDKWDFGGSDFRYFPFGSGRRNCAGIAMAERMVIY 537
           NVWAIQRDPN WENPLVF+PERFL+  KWDFGGSDFRYFPFGSGRRNCAGI MAER  +Y
Sbjct: 422 NVWAIQRDPNHWENPLVFDPERFLDNQKWDFGGSDFRYFPFGSGRRNCAGIGMAERTFMY 481

Query: 538 LLATLLHSFDWKLEDGEKMEVEEKFGIVMKMKSPLVLIPTPRLSDPTLY 582
           LLATLLHSFDWK+E+G+K+E+EEKFGIV+KMK+PLVLIPTPRL D TLY
Sbjct: 482 LLATLLHSFDWKVEEGKKLEIEEKFGIVLKMKTPLVLIPTPRLCDHTLY 530

BLAST of Lsi09G003840 vs. NCBI nr
Match: XP_038874643.1 (flavonoid 3'-monooxygenase CYP75B137-like [Benincasa hispida])

HSP 1 Score: 902.9 bits (2332), Expect = 1.4e-258
Identity = 447/536 (83.40%), Postives = 480/536 (89.55%), Query Frame = 0

Query: 47  KFSFFLHSAMEQPTSNSLLHLIDSLNSFFPWRSIDQSRFLFTFFAAILIFLYIKLKRRRL 106
           K SF LHSAME+ TSN                      FLFT FAA+LIFLY+KL+ R +
Sbjct: 22  KLSFILHSAMEELTSN----------------------FLFTVFAALLIFLYLKLRHRHM 81

Query: 107 PLPPGPRGVPLLGNLPFLDPELHTYFTKLGEKYGPIVKLQLGKKIGIIVNSPSVAREVLK 166
           PLPPGP G+PLLGNLPFLDPELHTYFTKLGE+YGPI+KLQLG K+GIIVNSP +AREVLK
Sbjct: 82  PLPPGPWGIPLLGNLPFLDPELHTYFTKLGERYGPILKLQLGNKVGIIVNSPWIAREVLK 141

Query: 167 DQDVTFANRDVPQAGRAATYGGSDIVWTPYGPEWRMLRKVCVLKMLSNTTLDSVYELRYR 226
           D DVTFANRDVP AG+AATYGGSDIVWTPYGPEWRMLRKVCVLKMLSN TLDSVYELR R
Sbjct: 142 DHDVTFANRDVPHAGKAATYGGSDIVWTPYGPEWRMLRKVCVLKMLSNATLDSVYELRRR 201

Query: 227 EVRNTVAHLYRRSGSAVNVGEQGFLTIFNVVTSMLWGGSVEGEQRDGLAAEFRETVSEIT 286
           EVRNT+AHLYRR+GS VNVGEQGFLT+FNVVTSMLWGGSVEGEQRDGLAAEFRETVS++T
Sbjct: 202 EVRNTIAHLYRRAGSVVNVGEQGFLTVFNVVTSMLWGGSVEGEQRDGLAAEFRETVSDMT 261

Query: 287 ELLGKPNVSDFFPSLARFDLQGIEKQMREIAPRFDNIFEKMIDQRLKMGNEDEDGNVKKN 346
           ELLGKPNVSDFFPSLARFDLQGIEK+MRE+APRFD IFEKMIDQRLK+  ED+  +VKKN
Sbjct: 262 ELLGKPNVSDFFPSLARFDLQGIEKKMRELAPRFDRIFEKMIDQRLKIVGEDDSRSVKKN 321

Query: 347 DFLQFLLKVKDEGESKTPLTMTHLKALLMDMVVGGTDTSSNTVEFAMAEMIKNPKTLKKA 406
           DFLQFLL+VK++GESKTPLTMTHLKALLMDMV+GGTDTSSNT+EFAMAEMIKNP+TLKKA
Sbjct: 322 DFLQFLLEVKEDGESKTPLTMTHLKALLMDMVIGGTDTSSNTIEFAMAEMIKNPETLKKA 381

Query: 407 QEEITAVVGEDNIVEESHIHSFPYLKAVMKETLRLHPILPLLVPHCPSETTIVSNYAIPK 466
           QEE+  VVGEDNIVEE HIHS PYLKAVMKETLRLHPILPLLVPHCPSETTIV+NY IPK
Sbjct: 382 QEELAVVVGEDNIVEEFHIHSLPYLKAVMKETLRLHPILPLLVPHCPSETTIVANYTIPK 441

Query: 467 GSRVFINVWAIQRDPNQWENPLVFEPERFLETDKWDFGGSDFRYFPFGSGRRNCAGIAMA 526
           GSRVFINVWAIQR PNQWENPL F PERFL   K+DF GSDFRYFPFGSGRRNCAGIAMA
Sbjct: 442 GSRVFINVWAIQRHPNQWENPLEFNPERFL-NGKFDFSGSDFRYFPFGSGRRNCAGIAMA 501

Query: 527 ERMVIYLLATLLHSFDWKLEDGEKMEVEEKFGIVMKMKSPLVLIPTPRLSDPTLYQ 583
           ERMV+YLLATLLHSFDWKLEDGEKMEVEEKFGIV+KMK PL+LIPTPRLS  TLYQ
Sbjct: 502 ERMVLYLLATLLHSFDWKLEDGEKMEVEEKFGIVLKMKRPLILIPTPRLSHFTLYQ 534

BLAST of Lsi09G003840 vs. NCBI nr
Match: XP_004147580.1 (flavonoid 3'-monooxygenase CYP75B137 [Cucumis sativus] >KGN50206.1 hypothetical protein Csa_000316 [Cucumis sativus])

HSP 1 Score: 894.0 bits (2309), Expect = 6.7e-256
Identity = 443/528 (83.90%), Postives = 482/528 (91.29%), Query Frame = 0

Query: 56  MEQPTSNSLLHLIDSLNSFFPWRSIDQSRFLFTFFAAILIFLYIKLKRRRLPLPPGPRGV 115
           ME PTSN           FF   S DQS F FTF AA+LIFLY++L R R+PLPPGPRGV
Sbjct: 1   MENPTSN-----------FF---SFDQSHFFFTFLAALLIFLYLRLTRLRVPLPPGPRGV 60

Query: 116 PLLGNLPFLDPELHTYFTKLGEKYGPIVKLQLGKKIGIIVNSPSVAREVLKDQDVTFANR 175
           PLLGNLPFL PELHTYF +LG+KYGPIVKLQLG+KIGII+NSPSV REVLKD DVTFANR
Sbjct: 61  PLLGNLPFLHPELHTYFAQLGQKYGPIVKLQLGRKIGIIINSPSVVREVLKDHDVTFANR 120

Query: 176 DVPQAGRAATYGGSDIVWTPYGPEWRMLRKVCVLKMLSNTTLDSVYELRYREVRNTVAHL 235
           DVPQAGRAA+YGGSDIVWTPYGP+WRMLRKVCV+KMLSN TLDSVYELR +EVRNTVAHL
Sbjct: 121 DVPQAGRAASYGGSDIVWTPYGPKWRMLRKVCVVKMLSNATLDSVYELRRKEVRNTVAHL 180

Query: 236 YRRSGSAVNVGEQGFLTIFNVVTSMLWGGSVEGEQRDGLAAEFRETVSEITELLGKPNVS 295
           Y R+G+AVNVGEQGFLT+FNVVTSMLWGGSVEGEQRDGLAAEFRETVSE+TELLG PNVS
Sbjct: 181 YARAGTAVNVGEQGFLTVFNVVTSMLWGGSVEGEQRDGLAAEFRETVSEMTELLGLPNVS 240

Query: 296 DFFPSLARFDLQGIEKQMREIAPRFDNIFEKMIDQRLKMGNEDEDGNVKKNDFLQFLLKV 355
           DFFPSLARFDLQGIEK+MRE+APRFD+IFEKMIDQRLK+   DE G+VKKNDFLQFLL+V
Sbjct: 241 DFFPSLARFDLQGIEKKMRELAPRFDSIFEKMIDQRLKIDGGDEGGSVKKNDFLQFLLQV 300

Query: 356 KDEGESKTPLTMTHLKALLMDMVVGGTDTSSNTVEFAMAEMIKNPKTLKKAQEEITAVVG 415
           KDE ESKTPLTMTHLKALLMDMV+GGTDTSSNTVEFAMAEM+K+PKTLKKAQEE+ AVVG
Sbjct: 301 KDEEESKTPLTMTHLKALLMDMVIGGTDTSSNTVEFAMAEMLKSPKTLKKAQEEVVAVVG 360

Query: 416 EDNIVEESHIHSFPYLKAVMKETLRLHPILPLLVPHCPSETTIVSNYAIPKGSRVFINVW 475
           EDNIVEESHIHS PYLKAVMKETLRLHPILPLLVPHCPSET IVSNY IPKGSRVFINVW
Sbjct: 361 EDNIVEESHIHSLPYLKAVMKETLRLHPILPLLVPHCPSETAIVSNYTIPKGSRVFINVW 420

Query: 476 AIQRDPNQWENPLVFEPERFLETDKWDFGGSDFRYFPFGSGRRNCAGIAMAERMVIYLLA 535
           AIQRDP  W+NPL F+PERFL   K+DF G+DFRYFPFGSGRRNCAGIAMAERMV+Y+LA
Sbjct: 421 AIQRDPKNWDNPLEFDPERFL-NGKFDFSGNDFRYFPFGSGRRNCAGIAMAERMVMYMLA 480

Query: 536 TLLHSFDWKLEDGE-KMEVEEKFGIVMKMKSPLVLIPTPRLSDPTLYQ 583
           TLLHSFDWKLE+G+ K+EVEEKFGIV+KMK+PLVLIPTP+L+DPTLYQ
Sbjct: 481 TLLHSFDWKLEEGDGKIEVEEKFGIVLKMKTPLVLIPTPKLADPTLYQ 513

BLAST of Lsi09G003840 vs. NCBI nr
Match: XP_008437138.1 (PREDICTED: flavonoid 3'-monooxygenase-like [Cucumis melo])

HSP 1 Score: 892.1 bits (2304), Expect = 2.5e-255
Identity = 442/528 (83.71%), Postives = 480/528 (90.91%), Query Frame = 0

Query: 56  MEQPTSNSLLHLIDSLNSFFPWRSIDQSRFLFTFFAAILIFLYIKLKRRRLPLPPGPRGV 115
           ME PTSN           FF   SIDQS F FTF AA+LIFLY++L R R+PLPPGPRGV
Sbjct: 1   MEDPTSN-----------FF---SIDQSHFFFTFLAALLIFLYLRLTRLRVPLPPGPRGV 60

Query: 116 PLLGNLPFLDPELHTYFTKLGEKYGPIVKLQLGKKIGIIVNSPSVAREVLKDQDVTFANR 175
           PLLGNLPFLDPELHTYF +L +KYGPIVKLQLG+KIGII+NSPSV REVLKD DVTFANR
Sbjct: 61  PLLGNLPFLDPELHTYFAQLSQKYGPIVKLQLGRKIGIIINSPSVVREVLKDHDVTFANR 120

Query: 176 DVPQAGRAATYGGSDIVWTPYGPEWRMLRKVCVLKMLSNTTLDSVYELRYREVRNTVAHL 235
           DVP AGRAA+YGGSDIVWTPYGP+WRMLRKVCVLKMLSN TLDSVYELR REVRNTVAHL
Sbjct: 121 DVPHAGRAASYGGSDIVWTPYGPQWRMLRKVCVLKMLSNATLDSVYELRRREVRNTVAHL 180

Query: 236 YRRSGSAVNVGEQGFLTIFNVVTSMLWGGSVEGEQRDGLAAEFRETVSEITELLGKPNVS 295
           Y R+G+ VNVGEQGFLT+FNVVTSMLWGGSVEGEQRDGLAAEFRETVSE+TELLG PNVS
Sbjct: 181 YARAGTRVNVGEQGFLTVFNVVTSMLWGGSVEGEQRDGLAAEFRETVSEMTELLGLPNVS 240

Query: 296 DFFPSLARFDLQGIEKQMREIAPRFDNIFEKMIDQRLKMGNEDEDGNVKKNDFLQFLLKV 355
           DFFPSLARFD+QGIEK+MRE+APRFD+IFEKMIDQRLK+  +DE  +VKKNDFLQFLL+V
Sbjct: 241 DFFPSLARFDIQGIEKKMRELAPRFDSIFEKMIDQRLKIDGKDEGESVKKNDFLQFLLQV 300

Query: 356 KDEGESKTPLTMTHLKALLMDMVVGGTDTSSNTVEFAMAEMIKNPKTLKKAQEEITAVVG 415
           KD+GESKTPLTMTHLKALLMDMV+GGTDTSSNTVEFAMAEM+K+PKTLKKAQ+E+ AVVG
Sbjct: 301 KDDGESKTPLTMTHLKALLMDMVIGGTDTSSNTVEFAMAEMLKSPKTLKKAQQELVAVVG 360

Query: 416 EDNIVEESHIHSFPYLKAVMKETLRLHPILPLLVPHCPSETTIVSNYAIPKGSRVFINVW 475
           EDNIVEESHIHS PYLKAVMKETLRLHPILPLLVPHCPSET IVSNY IPKGSRVFINVW
Sbjct: 361 EDNIVEESHIHSLPYLKAVMKETLRLHPILPLLVPHCPSETAIVSNYTIPKGSRVFINVW 420

Query: 476 AIQRDPNQWENPLVFEPERFLETDKWDFGGSDFRYFPFGSGRRNCAGIAMAERMVIYLLA 535
           AIQRDP  W+NPL F+PERFL   K+DF G+DFRYFPFGSGRRNCAGI MAERMV+YLLA
Sbjct: 421 AIQRDPKNWDNPLEFDPERFL-NGKYDFSGNDFRYFPFGSGRRNCAGIGMAERMVMYLLA 480

Query: 536 TLLHSFDWKLEDG-EKMEVEEKFGIVMKMKSPLVLIPTPRLSDPTLYQ 583
           TLLHSFDWKL DG EK+EVEEKFGIV+KMK+PLV+IPTP+LSDPTLYQ
Sbjct: 481 TLLHSFDWKLGDGDEKIEVEEKFGIVLKMKTPLVVIPTPKLSDPTLYQ 513

BLAST of Lsi09G003840 vs. NCBI nr
Match: XP_023549715.1 (geraniol 8-hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 880.6 bits (2274), Expect = 7.7e-252
Identity = 431/529 (81.47%), Postives = 475/529 (89.79%), Query Frame = 0

Query: 58  QPTSNSLLHLIDSLNSFFPWRSID----QSRFLFT-FFAAILIFLYIKLKRRRLPLPPGP 117
           + TSNSL  LIDSLN   PWR ID    QSR LFT FFA +LIF+Y K+ RRR+PLPPGP
Sbjct: 2   ETTSNSLQTLIDSLNFLLPWRWIDAANNQSRLLFTVFFAGLLIFVYTKVTRRRVPLPPGP 61

Query: 118 RGVPLLGNLPFLDPELHTYFTKLGEKYGPIVKLQLGKKIGIIVNSPSVAREVLKDQDVTF 177
            GVPLLGNLPFLDPELHTYF  LG+KYGPIVKLQLG K+GI+VNSPSVARE+LKD DVTF
Sbjct: 62  PGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIVVNSPSVAREILKDHDVTF 121

Query: 178 ANRDVPQAGRAATYGGSDIVWTPYGPEWRMLRKVCVLKMLSNTTLDSVYELRYREVRNTV 237
           ANRDVPQAGRAATYGGS+IVW PYG EWRMLRKVCV+KMLSN TL+SVY+LR REVRNTV
Sbjct: 122 ANRDVPQAGRAATYGGSNIVWNPYGSEWRMLRKVCVVKMLSNATLESVYDLRRREVRNTV 181

Query: 238 AHLYRRSGSAVNVGEQGFLTIFNVVTSMLWGGSVEGEQRDGLAAEFRETVSEITELLGKP 297
           A+LY R GSAVNVGEQ FLTIFNVVTSMLWGG +EG+QRD LAAEFRETVS++TELLGKP
Sbjct: 182 ANLYGRGGSAVNVGEQSFLTIFNVVTSMLWGGIMEGDQRDSLAAEFRETVSDLTELLGKP 241

Query: 298 NVSDFFPSLARFDLQGIEKQMREIAPRFDNIFEKMIDQRLKMGNEDEDGNVKKNDFLQFL 357
           NVSDFFPSLARFDLQGIEK+MR++A +FD IFE MI+QRLK+   ++  + + NDFLQFL
Sbjct: 242 NVSDFFPSLARFDLQGIEKKMRKLALKFDTIFENMINQRLKIVGGEDSESAQNNDFLQFL 301

Query: 358 LKVKDEGESKTPLTMTHLKALLMDMVVGGTDTSSNTVEFAMAEMIKNPKTLKKAQEEITA 417
           L+VKD G+SKTPLT+THLKALLMDMV+GGTDTSSNT+EFA+AEMI NP TLKKAQEE+ A
Sbjct: 302 LEVKDAGDSKTPLTITHLKALLMDMVIGGTDTSSNTIEFALAEMINNPATLKKAQEEVAA 361

Query: 418 VVGEDNIVEESHIHSFPYLKAVMKETLRLHPILPLLVPHCPSETTIVSNYAIPKGSRVFI 477
           VVGEDN+VEESHIHS PYLKAVMKETLRLHPILPLLVPH PSETTI+S Y IPKGSRVFI
Sbjct: 362 VVGEDNMVEESHIHSLPYLKAVMKETLRLHPILPLLVPHSPSETTIISKYTIPKGSRVFI 421

Query: 478 NVWAIQRDPNQWENPLVFEPERFLETDKWDFGGSDFRYFPFGSGRRNCAGIAMAERMVIY 537
           NVWAIQRDPN WENPLVF+PERFL   KWDFGGSDFRYFPFGSGRRNCAGIAMAER  +Y
Sbjct: 422 NVWAIQRDPNHWENPLVFDPERFLNNQKWDFGGSDFRYFPFGSGRRNCAGIAMAERTFMY 481

Query: 538 LLATLLHSFDWKLEDGEKMEVEEKFGIVMKMKSPLVLIPTPRLSDPTLY 582
           LLATLLHSFDWKLE+GEK+E+EEKFGIV+KMK+PLVLIPTPRLSDPTLY
Sbjct: 482 LLATLLHSFDWKLEEGEKLEIEEKFGIVLKMKTPLVLIPTPRLSDPTLY 530

BLAST of Lsi09G003840 vs. NCBI nr
Match: XP_023551603.1 (geraniol 8-hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 875.5 bits (2261), Expect = 2.5e-250
Identity = 430/530 (81.13%), Postives = 473/530 (89.25%), Query Frame = 0

Query: 58  QPTSNSLLHLIDSLNSFFPWRSI----DQSRFLFT-FFAAILIFLYIKLKRRRLPLPPGP 117
           + TSNSL  LIDSLN   PWR I    +QSR LFT FFA +LIFLY K+ RRR+ LPPGP
Sbjct: 2   ETTSNSLQTLIDSLNFLLPWRWIHAADNQSRLLFTVFFAGLLIFLYTKVTRRRVTLPPGP 61

Query: 118 RGVPLLGNLPFLDPELHTYFTKLGEKYGPIVKLQLGKKIGIIVNSPSVAREVLKDQDVTF 177
            GVPLLGNLPFLDPELHTYF  LG+KYGPIVKLQLG K+GIIVNSPSVARE+LKD DVTF
Sbjct: 62  LGVPLLGNLPFLDPELHTYFRDLGQKYGPIVKLQLGNKVGIIVNSPSVAREILKDHDVTF 121

Query: 178 ANRDVPQAGRAATYGGSDIVWTPYGPEWRMLRKVCVLKMLSNTTLDSVYELRYREVRNTV 237
           ANRDVPQAGRAATYGGSDI WTPYG EWRMLRKVCV+KMLSN TL+SVY+LR REVRNTV
Sbjct: 122 ANRDVPQAGRAATYGGSDIAWTPYGAEWRMLRKVCVVKMLSNATLESVYDLRRREVRNTV 181

Query: 238 AHLYRRSGSAVNVGEQGFLTIFNVVTSMLWGGSVEGEQRDGLAAEFRETVSEITELLGKP 297
           A+LY R GSAVNVGEQ FLTIFNVVTSMLWGG +EG+QRD LAAEFRETVS++TELLGKP
Sbjct: 182 ANLYGRGGSAVNVGEQSFLTIFNVVTSMLWGGIMEGDQRDSLAAEFRETVSDLTELLGKP 241

Query: 298 NVSDFFPSLARFDLQGIEKQMREIAPRFDNIFEKMIDQRLKMGNEDEDGNVKKNDFLQFL 357
           NVSDFFPSLARFDLQGIEK+M ++A +FD IFE MI+QRLK+   ++  + K  DFLQFL
Sbjct: 242 NVSDFFPSLARFDLQGIEKKMHKLAHKFDTIFENMINQRLKIAGGEDGESAKSKDFLQFL 301

Query: 358 LKVKDEGESKTPLTMTHLKALLMDMVVGGTDTSSNTVEFAMAEMIKNPKTLKKAQEEITA 417
           L+VKD G+SKTPLT+THLKALLMDMV GGTDTSSNT+EFA+AEMI +P TLKKAQEE+ A
Sbjct: 302 LEVKDSGDSKTPLTITHLKALLMDMVTGGTDTSSNTIEFALAEMINSPATLKKAQEEVAA 361

Query: 418 VVGEDNIVEESHIHSFPYLKAVMKETLRLHPILPLLVPHCPSETTIVSNYAIPKGSRVFI 477
           VVGEDN+VEESHIHS PYLKAVMKETLRLHPILPLLVPH PSETTI+S Y IPKGSRVFI
Sbjct: 362 VVGEDNMVEESHIHSLPYLKAVMKETLRLHPILPLLVPHSPSETTIISKYTIPKGSRVFI 421

Query: 478 NVWAIQRDPNQWENPLVFEPERFLETDKWDFGGSDFRYFPFGSGRRNCAGIAMAERMVIY 537
           NVWAIQRDPNQWENPLVF+PERFL   KWDFGGSDFRYFPFGSGRRNCAGIAMAER  +Y
Sbjct: 422 NVWAIQRDPNQWENPLVFDPERFLNNQKWDFGGSDFRYFPFGSGRRNCAGIAMAERTFMY 481

Query: 538 LLATLLHSFDWKLEDGEKMEVEEKFGIVMKMKSPLVLIPTPRLSDPTLYQ 583
           LLATLLHSF+WKLE+G+K+E+EEKFGIV+KMK+PLVLIPTPRLSDPTLYQ
Sbjct: 482 LLATLLHSFNWKLEEGKKLEIEEKFGIVLKMKTPLVLIPTPRLSDPTLYQ 531

BLAST of Lsi09G003840 vs. TAIR 10
Match: AT4G12300.1 (cytochrome P450, family 706, subfamily A, polypeptide 4 )

HSP 1 Score: 634.0 bits (1634), Expect = 1.2e-181
Identity = 302/515 (58.64%), Postives = 390/515 (75.73%), Query Frame = 0

Query: 71  LNSFFPWRSIDQSRFLFTFFAAILIFLYIKLKRRRLP-LPPGPRGVPLLGNLPFLDPELH 130
           +++ FP  +I+ + +       +   L+   KR   P LPPGPRG+P++GNLPFLDP+LH
Sbjct: 4   ISNLFPDNTINLTPYAIVILTTVFSILWYIFKRSPQPSLPPGPRGLPIVGNLPFLDPDLH 63

Query: 131 TYFTKLGEKYGPIVKLQLGKKIGIIVNSPSVAREVLKDQDVTFANRDVPQAGRAATYGGS 190
           TYF  L + +GPI KL LG K+ I+VNSPS+ARE+LKDQD+ F+NRDVP  GRAATYGG 
Sbjct: 64  TYFANLAQSHGPIFKLNLGSKLTIVVNSPSLAREILKDQDINFSNRDVPLTGRAATYGGI 123

Query: 191 DIVWTPYGPEWRMLRKVCVLKMLSNTTLDSVYELRYREVRNTVAHLYR--RSGSAVNVGE 250
           DIVWTPYG EWR LRK+CVLK+LS  TLDS YELR +EVR    +LY   R  S V VG+
Sbjct: 124 DIVWTPYGAEWRQLRKICVLKLLSRKTLDSFYELRRKEVRERTRYLYEQGRKQSPVKVGD 183

Query: 251 QGFLTIFNVVTSMLWGGSVEGEQRDGLAAEFRETVSEITELLGKPNVSDFFPSLARFDLQ 310
           Q FLT+ N+  +MLWGGSV+ E+ + +  EF+  +SEIT LL +PNVSDFFP LARFDLQ
Sbjct: 184 QLFLTMMNLTMNMLWGGSVKAEEMESVGTEFKGVISEITRLLSEPNVSDFFPWLARFDLQ 243

Query: 311 GIEKQMREIAPRFDNIFEKMIDQRLKMGNEDEDGNVKKNDFLQFLLKVKD-EGESKTPLT 370
           G+ K+M   A   D + ++ I+Q   +   D+D   +  DFLQ+L+K+KD EG+S+ P+T
Sbjct: 244 GLVKRMGVCARELDAVLDRAIEQMKPLRGRDDD---EVKDFLQYLMKLKDQEGDSEVPIT 303

Query: 371 MTHLKALLMDMVVGGTDTSSNTVEFAMAEMIKNPKTLKKAQEEITAVVGEDNIVEESHIH 430
           + H+KALL DMVVGGTDTS+NT+EFAMAE++ NP+ +K+AQEE+  VVG+DNIVEESHI 
Sbjct: 304 INHVKALLTDMVVGGTDTSTNTIEFAMAELMSNPELIKRAQEELDEVVGKDNIVEESHIT 363

Query: 431 SFPYLKAVMKETLRLHPILPLLVPHCPSETTIVSNYAIPKGSRVFINVWAIQRDPNQWEN 490
             PY+ A+MKETLRLHP LPLLVPH P+E T+V  Y IPK +++F+NVW+IQRDPN WEN
Sbjct: 364 RLPYILAIMKETLRLHPTLPLLVPHRPAENTVVGGYTIPKDTKIFVNVWSIQRDPNVWEN 423

Query: 491 PLVFEPERFLETDKWDFGGSDFRYFPFGSGRRNCAGIAMAERMVIYLLATLLHSFDWKLE 550
           P  F PERFL+ +  DF G+++ YFPFGSGRR CAG+A+AERMV+Y LATLLHSFDWK+ 
Sbjct: 424 PTEFRPERFLDNNSCDFTGANYSYFPFGSGRRICAGVALAERMVLYTLATLLHSFDWKIP 483

Query: 551 DGEKMEVEEKFGIVMKMKSPLVLIPTPRLSDPTLY 582
           +G  ++++EKFGIV+K+K PLV +P PR SD  LY
Sbjct: 484 EGHVLDLKEKFGIVLKLKIPLVALPIPRFSDSNLY 515

BLAST of Lsi09G003840 vs. TAIR 10
Match: AT4G12320.1 (cytochrome P450, family 706, subfamily A, polypeptide 6 )

HSP 1 Score: 627.9 bits (1618), Expect = 8.3e-180
Identity = 300/516 (58.14%), Postives = 394/516 (76.36%), Query Frame = 0

Query: 71  LNSFFPWRSIDQSRFLFTFFAAILIFLYIKLKRRRLP-LPPGPRGVPLLGNLPFLDPELH 130
           +++ F   +I+ + +      AI   L+   KR   P LPPGPRG+P++GNLPFLDP+LH
Sbjct: 4   ISNLFAGNTINLTPYAILILIAIFSILWYLFKRSPQPHLPPGPRGLPIVGNLPFLDPDLH 63

Query: 131 TYFTKLGEKYGPIVKLQLGKKIGIIVNSPSVAREVLKDQDVTFANRDVPQAGRAATYGGS 190
           TYFTKL E YGPI KL LG K+ ++VN+PS+ARE+LKDQD+ F+N DVP   RA TYGG 
Sbjct: 64  TYFTKLAESYGPIFKLNLGSKLTVVVNTPSLAREILKDQDINFSNHDVPLTARAVTYGGL 123

Query: 191 DIVWTPYGPEWRMLRKVCVLKMLSNTTLDSVYELRYREVRNTVAHLYRR--SGSAVNVGE 250
           D+VW PYG EWRMLRKVCVLK+LS+ TL+S YELR +E+R    +LY++    S VNVGE
Sbjct: 124 DLVWLPYGAEWRMLRKVCVLKLLSHRTLNSFYELRRKEIRERTRYLYQKGQEESPVNVGE 183

Query: 251 QGFLTIFNVVTSMLWGGSVEGEQRDGLAAEFRETVSEITELLGKPNVSDFFPSLARFDLQ 310
           Q FLT+ N+  +MLWGGSV+ E+ + +  EF+E +SEIT LLG+PNVSDFFP LARFDLQ
Sbjct: 184 QVFLTMMNLTMNMLWGGSVKAEEMESVGTEFKEVISEITRLLGEPNVSDFFPRLARFDLQ 243

Query: 311 GIEKQMREIAPRFDNIFEKMIDQ-RLKMGNEDEDGNVKKNDFLQFLLKVKD-EGESKTPL 370
           G+ K+M   A   D I ++ I+Q +L    + +DG  K  DFLQ L+K+KD E +S+ P+
Sbjct: 244 GLVKKMHVCARELDAILDRAIEQMQLLRTRDGDDGECK--DFLQHLMKLKDQEADSEVPI 303

Query: 371 TMTHLKALLMDMVVGGTDTSSNTVEFAMAEMIKNPKTLKKAQEEITAVVGEDNIVEESHI 430
           T+ H+KA+L+D+VVGGTDTS+NT+EFAMAE+I+ P+ +K+AQ+E+  VVG+DNI+EESHI
Sbjct: 304 TVNHVKAVLVDLVVGGTDTSTNTIEFAMAELIRKPELMKRAQQELDEVVGKDNIIEESHI 363

Query: 431 HSFPYLKAVMKETLRLHPILPLLVPHCPSETTIVSNYAIPKGSRVFINVWAIQRDPNQWE 490
              P++ A+MKETLRL+P +PLLVPH PSET +V  Y IPK +++FINVW+IQRDPN WE
Sbjct: 364 TRLPFISAIMKETLRLYPTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNVWE 423

Query: 491 NPLVFEPERFLETDKWDFGGSDFRYFPFGSGRRNCAGIAMAERMVIYLLATLLHSFDWKL 550
            P  F PERFL+    DF G+D+ Y PFGSGRR CAGIA+AERM++Y LATLLHSFDWK+
Sbjct: 424 YPTEFRPERFLDKKSCDFTGTDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDWKI 483

Query: 551 EDGEKMEVEEKFGIVMKMKSPLVLIPTPRLSDPTLY 582
            +G  ++++EKFGIV+K+KSPLV +P PRLS+  LY
Sbjct: 484 PEGHILDLKEKFGIVLKLKSPLVALPVPRLSNSNLY 517

BLAST of Lsi09G003840 vs. TAIR 10
Match: AT4G12310.1 (cytochrome P450, family 706, subfamily A, polypeptide 5 )

HSP 1 Score: 613.6 bits (1581), Expect = 1.6e-175
Identity = 297/518 (57.34%), Postives = 385/518 (74.32%), Query Frame = 0

Query: 71  LNSFFPWRSIDQSRFLFTFF---AAILIFLYIKLKRRRLPLPPGPRGVPLLGNLPFLDPE 130
           L++ FP  +I  + + +      A   I  YI  +  + PLPPGPRG+P++GNLPFLDP+
Sbjct: 4   LSNLFPDNAISLTPYAYAVLILTATFSILWYIFKRSPQPPLPPGPRGLPIVGNLPFLDPD 63

Query: 131 LHTYFTKLGEKYGPIVKLQLGKKIGIIVNSPSVAREVLKDQDVTFANRDVPQAGRAATYG 190
           LHTYFTKL + +GPI KL LG K+ ++VNSPS+A E+LKDQD+ F+N DVP   RA TYG
Sbjct: 64  LHTYFTKLAQSHGPIFKLNLGSKLTVVVNSPSLASEILKDQDINFSNHDVPLTARAVTYG 123

Query: 191 GSDIVWTPYGPEWRMLRKVCVLKMLSNTTLDSVYELRYREVRNTVAHLYRR--SGSAVNV 250
           G D+VW PYG EWRMLRKVC  K+ S  TLDS YELR +E+R     LY++    S VNV
Sbjct: 124 GLDLVWLPYGAEWRMLRKVCAAKLFSRKTLDSFYELRRKEIRERTRCLYQKGLEKSPVNV 183

Query: 251 GEQGFLTIFNVVTSMLWGGSVEGEQRDGLAAEFRETVSEITELLGKPNVSDFFPSLARFD 310
           GEQ FLT+ N++ +MLWGGSV+ E  + +  EF+  +SEIT LLG PNVSDFFP LARFD
Sbjct: 184 GEQLFLTMMNLMMNMLWGGSVKAEDMESVGTEFKGVISEITRLLGVPNVSDFFPMLARFD 243

Query: 311 LQGIEKQMREIAPRFDNIFEKMIDQRLKMGNED-EDGNVKKNDFLQFLLKVKD-EGESKT 370
           LQG+ K+M   A   D I ++ I+Q  ++ + D +DG  K  DFLQ L+K++D E +S  
Sbjct: 244 LQGLVKKMHLYARDLDAILDRAIEQMQRLRSRDGDDGECK--DFLQHLMKLRDQEADSDV 303

Query: 371 PLTMTHLKALLMDMVVGGTDTSSNTVEFAMAEMIKNPKTLKKAQEEITAVVGEDNIVEES 430
           P+TM H+KA+LMDMVVGGT++S+NT+EF MAE+I NP+ +++AQ+E+  VVG+DNIVEES
Sbjct: 304 PITMNHVKAVLMDMVVGGTESSTNTIEFVMAELISNPELMRRAQQELDEVVGKDNIVEES 363

Query: 431 HIHSFPYLKAVMKETLRLHPILPLLVPHCPSETTIVSNYAIPKGSRVFINVWAIQRDPNQ 490
           HI S PY+ AV+KETLRL+P +PLLVPH PSET +V  Y IPK +++FINVW+IQRDPN 
Sbjct: 364 HITSLPYILAVLKETLRLYPTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNV 423

Query: 491 WENPLVFEPERFLETDKWDFGGSDFRYFPFGSGRRNCAGIAMAERMVIYLLATLLHSFDW 550
           WE P  F PERFL+    DF G+D+ Y PFGSGRR CAGIA+AERM++Y LATLLHSFDW
Sbjct: 424 WEYPTEFRPERFLDKKSCDFTGTDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDW 483

Query: 551 KLEDGEKMEVEEKFGIVMKMKSPLVLIPTPRLSDPTLY 582
            + DG  +++EEKFGIV+K+K+PLV +P PRLS+   Y
Sbjct: 484 TIPDGHVLDLEEKFGIVLKLKTPLVALPIPRLSNSNFY 519

BLAST of Lsi09G003840 vs. TAIR 10
Match: AT4G12330.1 (cytochrome P450, family 706, subfamily A, polypeptide 7 )

HSP 1 Score: 594.0 bits (1530), Expect = 1.3e-169
Identity = 291/492 (59.15%), Postives = 375/492 (76.22%), Query Frame = 0

Query: 95  IFLYIKLKRRRLPLPPGPRGVPLLGNLPFLDPELHTYFTKLGEKYGPIVKLQLGKKIGII 154
           I++Y+K KR   PLPPGPRG+P++GNLPFL PELHTYF  L +K+GP+ KL LG K+ I+
Sbjct: 33  IWVYVKSKRLFPPLPPGPRGLPIVGNLPFLHPELHTYFHSLAQKHGPVFKLWLGAKLTIV 92

Query: 155 VNSPSVAREVLKDQDVTFANRDVPQAGRAATYGGSDIVWTPYGPEWRMLRKVCVLKMLSN 214
           + S    R++L+  DV FAN DVP AG  +TYGG DIVW+PYGPEW MLRK+C+ KMLSN
Sbjct: 93  ITSSEATRDILRTNDVIFANDDVPVAGSLSTYGGVDIVWSPYGPEWPMLRKICINKMLSN 152

Query: 215 TTLD--SVYELRYREVRNTVAHL--YRRSGSAVNVGEQGFLTIFNVVTSMLWGGSV-EGE 274
            TLD  S   LR +E R TV +L    R+G AVNVGEQ F+TI NVVT MLWG +V + E
Sbjct: 153 ATLDSNSFSALRRQETRRTVRYLADRARAGLAVNVGEQIFVTILNVVTQMLWGETVADDE 212

Query: 275 QRDGLAAEFRETVSEITELLGKPNVSDFFPSLARFDLQGIEKQMREIAPRFDNIFEKMID 334
           +R+ + AEF E ++EI +++GKPNVSDFFP L+RFDLQG+ K++R  A R D +F+++I 
Sbjct: 213 EREKVGAEFLELITEIIDVVGKPNVSDFFPVLSRFDLQGLAKRVRRSAQRMDRMFDRIIS 272

Query: 335 QRLKMGNEDEDGNVKKNDFLQFLLKVKDEGESKTPLTMTHLKALLMDMVVGGTDTSSNTV 394
           QR+ M   D+       DFL  LL  KDE E+   ++M H+KALLMDMV+GGTDTS NT+
Sbjct: 273 QRMGM---DKGSKGNGGDFLMVLLNAKDEDEN---MSMNHVKALLMDMVLGGTDTSLNTI 332

Query: 395 EFAMAEMIKNPKTLKKAQEEITAVVGEDNIVEESHIHSFPYLKAVMKETLRLHPILPLLV 454
           EFAMAE+I   + +K+AQ+E+  VVG++NIVEE HI   PY+ ++MKETLRLHP LPLL+
Sbjct: 333 EFAMAELINKLEIMKRAQQELDKVVGKNNIVEEKHITKLPYILSIMKETLRLHPALPLLI 392

Query: 455 PHCPSETTIVSNYAIPKGSRVFINVWAIQRDPNQWENPLVFEPERFLETDKWDFGGSDFR 514
           P CPSETT++  Y IP  S+VFINVWAI R+PN WENPL F P+RFL+   +DF G+D+ 
Sbjct: 393 PRCPSETTVIGGYTIPNDSKVFINVWAIHRNPNVWENPLEFNPDRFLDKG-YDFSGNDYS 452

Query: 515 YFPFGSGRRNCAGIAMAERMVIYLLATLLHSFDWKLEDGEKMEVEEKFGIVMKMKSPLVL 574
           YFPFGSGRR CAG+AMAE++V+Y LATLLHSFDW++ +GEK+E+EEKFGI++K+K+PLV 
Sbjct: 453 YFPFGSGRRICAGMAMAEKVVLYNLATLLHSFDWRIGEGEKVELEEKFGILLKLKNPLVA 512

Query: 575 IPTPRLSDPTLY 582
            P  RLSDP L+
Sbjct: 513 TPVLRLSDPNLF 517

BLAST of Lsi09G003840 vs. TAIR 10
Match: AT5G44620.1 (cytochrome P450, family 706, subfamily A, polypeptide 3 )

HSP 1 Score: 593.6 bits (1529), Expect = 1.7e-169
Identity = 287/489 (58.69%), Postives = 368/489 (75.26%), Query Frame = 0

Query: 95  IFLYIKLKRRRLPLPPGPRGVPLLGNLPFLDPELHTYFTKLGEKYGPIVKLQLGKKIGII 154
           ++LY K KRR  PLPPGP G+P++GNLPFL PELHTYF  L +K+GPI KL LG K+ I+
Sbjct: 33  LWLYAKCKRRSPPLPPGPWGLPIIGNLPFLQPELHTYFQGLAKKHGPIFKLWLGAKLTIV 92

Query: 155 VNSPSVAREVLKDQDVTFANRDVPQAGRAATYGGSDIVWTPYGPEWRMLRKVCVLKMLSN 214
           V S  VA+E+LK  D+ FAN DVP  G   TYGG++I+W+PYGP+WRMLRK+CV ++L N
Sbjct: 93  VTSSEVAQEILKTNDIIFANHDVPAVGPVNTYGGTEIIWSPYGPKWRMLRKLCVNRILRN 152

Query: 215 TTLDSVYELRYREVRNTVAHL--YRRSGSAVNVGEQGFLTIFNVVTSMLWGGSVEGEQRD 274
             LDS  +LR RE R TV +L    R GS VN+GEQ FL + NVVT MLWG +V+ E+R+
Sbjct: 153 AMLDSSTDLRRRETRQTVRYLADQARVGSPVNLGEQIFLMMLNVVTQMLWGTTVKEEERE 212

Query: 275 GLAAEFRETVSEITELLGKPNVSDFFPSLARFDLQGIEKQMREIAPRFDNIFEKMIDQRL 334
            + AEF E + E+ +LL  PN+SDFFP L+RFDLQG+ K+MR  A R D +F+++I+QRL
Sbjct: 213 VVGAEFLEVIREMNDLLLVPNISDFFPVLSRFDLQGLAKRMRRPAQRMDQMFDRIINQRL 272

Query: 335 KMGNEDEDGNVKKNDFLQFLLKVKDEGESKTPLTMTHLKALLMDMVVGGTDTSSNTVEFA 394
            M  +  DG  +  DFL  LLKVKDE   KT LTM  +KA+LMDMV+GGTDTS + +EFA
Sbjct: 273 GMDRDSSDG--RAVDFLDVLLKVKDEEAEKTKLTMNDVKAVLMDMVLGGTDTSLHVIEFA 332

Query: 395 MAEMIKNPKTLKKAQEEITAVVGEDNIVEESHIHSFPYLKAVMKETLRLHPILPLLVPHC 454
           MAE++ NP  +K+AQ+E+  VVG++ +VEESHI   PY+ A+MKETLRLH + PLLVP  
Sbjct: 333 MAELLHNPDIMKRAQQEVDKVVGKEKVVEESHISKLPYILAIMKETLRLHTVAPLLVPRR 392

Query: 455 PSETTIVSNYAIPKGSRVFINVWAIQRDPNQWENPLVFEPERFLETDKWDFGGSDFRYFP 514
           PS+TT+V  + IPK S++FIN WAI R+PN WENPL F+P+RFL+   +DF G+DF Y P
Sbjct: 393 PSQTTVVGGFTIPKDSKIFINAWAIHRNPNVWENPLKFDPDRFLDM-SYDFKGNDFNYLP 452

Query: 515 FGSGRRNCAGIAMAERMVIYLLATLLHSFDWKLEDGEKMEVEEKFGIVMKMKSPLVLIPT 574
           FGSGRR C G+AM ER+V+Y LAT LHSFDWK+  GE++EVEEKFGIV+++K+PLV  P 
Sbjct: 453 FGSGRRICVGMAMGERVVLYNLATFLHSFDWKIPQGERVEVEEKFGIVLELKNPLVATPV 512

Query: 575 PRLSDPTLY 582
            RLSDP LY
Sbjct: 513 LRLSDPNLY 518

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A4D6Q4153.7e-10039.49Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN... [more]
Q8VWZ71.6e-9839.72Geraniol 8-hydroxylase OS=Catharanthus roseus OX=4058 GN=CYP76B6 PE=1 SV=1[more]
D1MI463.9e-9739.47Geraniol 8-hydroxylase OS=Swertia mussotii OX=137888 GN=CYP76B10 PE=1 SV=1[more]
Q9SD856.6e-9739.60Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP75B1 PE=1 SV=1[more]
Q9SBQ95.6e-9638.97Flavonoid 3'-monooxygenase OS=Petunia hybrida OX=4102 GN=CYP75B2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KR553.2e-25683.90Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G159600 PE=3 SV=1[more]
A0A1S3ASY01.2e-25583.71flavonoid 3'-monooxygenase-like OS=Cucumis melo OX=3656 GN=LOC103482651 PE=3 SV=... [more]
A0A6J1E5N31.0e-24980.72geraniol 8-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111430178 PE=3 S... [more]
A0A6J1I0011.7e-24980.57geraniol 8-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111469168 PE=3 SV=... [more]
A0A6J1HYH51.3e-24479.58geraniol 8-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111469169 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
XP_038874643.11.4e-25883.40flavonoid 3'-monooxygenase CYP75B137-like [Benincasa hispida][more]
XP_004147580.16.7e-25683.90flavonoid 3'-monooxygenase CYP75B137 [Cucumis sativus] >KGN50206.1 hypothetical ... [more]
XP_008437138.12.5e-25583.71PREDICTED: flavonoid 3'-monooxygenase-like [Cucumis melo][more]
XP_023549715.17.7e-25281.47geraniol 8-hydroxylase-like [Cucurbita pepo subsp. pepo][more]
XP_023551603.12.5e-25081.13geraniol 8-hydroxylase-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT4G12300.11.2e-18158.64cytochrome P450, family 706, subfamily A, polypeptide 4 [more]
AT4G12320.18.3e-18058.14cytochrome P450, family 706, subfamily A, polypeptide 6 [more]
AT4G12310.11.6e-17557.34cytochrome P450, family 706, subfamily A, polypeptide 5 [more]
AT4G12330.11.3e-16959.15cytochrome P450, family 706, subfamily A, polypeptide 7 [more]
AT5G44620.11.7e-16958.69cytochrome P450, family 706, subfamily A, polypeptide 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 511..520
score: 57.11
coord: 380..397
score: 28.44
coord: 433..444
score: 48.01
IPR001128Cytochrome P450PFAMPF00067p450coord: 109..566
e-value: 5.0E-103
score: 345.3
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 432..450
score: 40.87
coord: 520..543
score: 29.44
coord: 369..386
score: 31.04
coord: 389..415
score: 32.19
coord: 510..520
score: 52.1
coord: 252..270
score: 19.07
coord: 473..497
score: 41.4
coord: 136..155
score: 27.89
coord: 160..181
score: 26.41
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 97..569
e-value: 3.2E-125
score: 420.4
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 109..574
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 90..548
e-value: 2.5E-18
score: 64.1
NoneNo IPR availablePIRSRPIRSR000209-1PIRSR000209-1coord: 110..556
e-value: 6.9E-42
score: 141.0
NoneNo IPR availablePANTHERPTHR47951:SF3CYTOCHROME P450, FAMILY 706, SUBFAMILY A, POLYPEPTIDE 3-RELATEDcoord: 83..582
NoneNo IPR availablePANTHERPTHR47951OS08G0547900 PROTEINcoord: 83..582
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 513..522

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G003840.1Lsi09G003840.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen