Lsi08G013910 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi08G013910 vs. ExPASy Swiss-Prot
Match: B2HIN2 (Long-chain-fatty-acid--AMP ligase FadD26 OS=Mycobacterium marinum (strain ATCC BAA-535 / M) OX=216594 GN=fadD26 PE=1 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 2.9e-45
Identity = 176/625 (28.16%), Postives = 282/625 (45.12%), Query Frame = 0

Query: 17  PVVDRYLPVWASLPAFR--SKPAFIWSEDGTADAVNEGSFLTYRQLHDSVQCISDRLLRQ 76
           PV DR +P      A +  ++ AF +  D   D       LT+ Q++     ++D L   
Sbjct: 2   PVTDRSIPSLLKEQADQRPNETAFTFL-DYDLDPNGFAETLTWSQVYARACVVADELTMY 61

Query: 77  LRRRDTVVVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCQHLARAFSQTKPRA 136
               D V +L   GLE +    G  +AG + VP+S P   + +E    + R    ++P A
Sbjct: 62  GVPGDRVAILAPQGLEYIVAFLGALQAGFIGVPLSTPQYGVHDERVSAVLR---DSQPVA 121

Query: 137 AIAHQSYINTIFRYLSSSPSDEKLALLLQSVKWISMDSLKQPHKEVEVNQNKHQPILYHS 196
            +   + +  + +Y SS   D + A  +  V  + +D+ +         Q   QP    S
Sbjct: 122 ILTTSAVVGDVTKYASS--QDGQPAPSVIEVDLLDLDTPRP-------QQALPQPA---S 181

Query: 197 SSYYGCKPEETYLIQYTSGATAIPKPVVITAGAAAHNVRAARKAY----DLNPND-VIVS 256
            S Y         +QYTSG+T  P  V+++      NV  +   Y    D  P D  +VS
Sbjct: 182 GSAY---------LQYTSGSTRTPAGVIVSHENVIANVTQSLYGYFGGPDKFPADTTVVS 241

Query: 257 WLPQYHDCGLMF-LLLTVITGATCVLTSPISFVTRPTTWLHLITAFKATCTPVPSFTLPL 316
           WLP +HD GL+  +   ++TG T VL SP+SF+ RP  W+ L+ +     +  P+F   L
Sbjct: 242 WLPLFHDMGLILGICAPLVTGCTAVLLSPMSFLRRPARWMQLLASHPKCFSAAPNFAFEL 301

Query: 317 VLKRVKEETSSSRGLDLGSLRNLILINEPIYRSAVEEFVDVFKAVGLDPGCVSPSYGLAE 376
            ++R  +E  +  GLDLG +  ++  +E I+ + ++ F + F    L P  V PSYGLAE
Sbjct: 302 AVRRTTDEDLA--GLDLGDVLGIVSGSERIHVATIKRFTERFAPFNLSPAAVRPSYGLAE 361

Query: 377 NCTFVSTAWCGGGDRRWLFPAMASYRKL-------------LPSARLRDGSCTEIEVVVV 436
              +V+    G   R   F     Y  L             + +  +  GS     V +V
Sbjct: 362 ATLYVAAPEPGTTPRTVRF----DYESLTAGHARPCRADGSVGTELISYGSPDPSAVRIV 421

Query: 437 NGETGEVVEDGVEGEIWVSSPSNATAFDESYRYLEHPSLSRETFNSKINNKS--SPN--F 496
           N ET      G  GEIW      A        Y + P  S  TFN++I N +  +P   +
Sbjct: 422 NPETMIENPSGTVGEIWAHGEHVAMG------YWQKPEQSDRTFNARIVNPAPGTPEGPW 481

Query: 497 VRTGDRGVIKGADRFLFVIGRCSDVIKFNNNQQEIHPHYIESIAYNNSSAYLRGGCIAAV 556
           +RTGD GV+   +  LF++GR  D++  +        HY + I    +   + GG +AA+
Sbjct: 482 LRTGDLGVMSNGE--LFIMGRIKDLVIVDGRN-----HYPDDI--EATIQEITGGRVAAI 541

Query: 557 ----KISETIALVAEMQRDDKNDVEL---LKNICQEIRKALLIEEGIELGVVVLVKRGNV 610
                I+E +  + E++R   +  E    L+++ +EI  A+     + +  VVLV  G++
Sbjct: 542 AVPDNITEQLVAIIELKRRGASAEEAMVKLRSVKREITSAISKSHSLRVADVVLVPPGSI 580

BLAST of Lsi08G013910 vs. ExPASy Swiss-Prot
Match: B2HIL4 (4-hydroxyphenylalkanoate adenylyltransferase OS=Mycobacterium marinum (strain ATCC BAA-535 / M) OX=216594 GN=fadD29 PE=1 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 1.6e-43
Identity = 156/575 (27.13%), Postives = 261/575 (45.39%), Query Frame = 0

Query: 55  LTYRQLHDSVQCISDRLLRQLRRRDTVVVLCSAGLELVQLIYGCQRAGLVSVPISPPDPF 114
           LT+ Q++   + +++ L       D V VL   GLE +    G   AGL++VP+  P   
Sbjct: 79  LTWWQIYRRAKIVAEELRGYGASGDRVAVLAPQGLEYIIAFLGVLEAGLIAVPLPVPQFG 138

Query: 115 LENENCQHLARAFSQTKPRAAIAHQSYINTIFRYLSSSPSDEKLALLLQSVKWISMDSLK 174
           + +E    ++ A   + P   +     I+ + +Y   + + +    ++ +V  + +DS  
Sbjct: 139 IHDE---RISAALQDSTPSVILTTSPVIDEVTKYAPHARAGQGGTPIVVAVDLLDLDSA- 198

Query: 175 QPHKEVEVNQNKHQPILYHSSSYYGCKPEETYLIQYTSGATAIPKPVVITAGAAAHNVRA 234
              +E+++    H    Y               +QYTSG+T  P  VV++      N   
Sbjct: 199 ---RELDLTPPAHSSTAY---------------LQYTSGSTRSPAGVVLSHKNVITNCVQ 258

Query: 235 ARKAYDLNPNDV---IVSWLPQYHDCGLMF-LLLTVITGATCVLTSPISFVTRPTTWLHL 294
               Y      V    VSWLP YHD GLM  ++L +I   T VL +P++F+ RP  W+ L
Sbjct: 259 LMSDYLGETEKVPSTAVSWLPFYHDMGLMLGIILPMINQDTAVLLNPMAFLQRPARWMQL 318

Query: 295 ITAFKATCTPVPSFTLPLVLKRVKEETSSSRGLDLGSLRNLILINEPIYRSAVEEFVDVF 354
           +  F+   +  P+F   L ++R  +E  +  GLDLG +R +    E +  + ++ F+D F
Sbjct: 319 MGKFRGQISCAPNFGFELAVRRTSDEDMA--GLDLGHVRGIGSGAERVNPATLQRFIDRF 378

Query: 355 KAVGLDPGCVSPSYGLAENCTFVSTAWCGGGDRRWLFPAMA-SYRKLLPSARLRD----- 414
               L    + PSYGLAE   FV+TA  G   R   F   + S  ++   A   D     
Sbjct: 379 APFNLRDTAIRPSYGLAEATVFVATAEPGRPPRSVNFDYQSLSVGRVERCANEADDGAKL 438

Query: 415 ---GSCTEIEVVVVNGETGEVVEDGVEGEIWVSSPSNATAFDESYRYLEHPSLSRETFNS 474
              GS    EV +V+ E       G  GEIWV   + A        Y  +P L+  TF++
Sbjct: 439 VSYGSSWTSEVRIVDPEARTECPAGTVGEIWVQGDNVAMG------YWRNPQLTERTFDA 498

Query: 475 KINNKSSPN----FVRTGDRGVIKGADRFLFVIGRCSDVIKFNNNQQEIHPHYIESIAYN 534
           K+ + S       ++RTGD GV+   +  LF+ GR  D++  + +      HY + I   
Sbjct: 499 KLTDPSPGTSIGPWLRTGDLGVMFEGE--LFITGRIKDLLIVDGSN-----HYPDDI--E 558

Query: 535 NSSAYLRGGCIAAVKISE-------TIALVAEMQRDDKNDVELLKNICQEIRKALLIEEG 594
           ++   + GG + A+ + +       TI   A      +  ++ L+++ +EI  A+     
Sbjct: 559 STIQEITGGRVVAIAVPDADGEKLVTIVEFASWGHSGQEAIDKLRSVKREITSAISRAHR 614

Query: 595 IELGVVVLVKRGNVPKTTSGKVKRWAVKEKLAGGG 606
           + +  VVLV  G++P TTSGKV+R +  E+    G
Sbjct: 619 VRVADVVLVATGSIPVTTSGKVRRSSCAERYRNDG 614

BLAST of Lsi08G013910 vs. ExPASy Swiss-Prot
Match: P9WQ44 (Putative fatty-acid--CoA ligase fadD25 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=fadD25 PE=3 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 3.5e-43
Identity = 153/566 (27.03%), Postives = 253/566 (44.70%), Query Frame = 0

Query: 55  LTYRQLHDSVQCISDRLLRQLRRRDTVVVLCSAGLELVQLIYGCQRAGLVSVPISPPDPF 114
           LT+ QL+     ++ +L       D  ++L    L+ V       +AG+V+VP+S P   
Sbjct: 41  LTWSQLYRRTLNLAAQLREHGSTGDRALILAPQSLDYVVSFIASLQAGIVAVPLSIPQGG 100

Query: 115 LENENCQHLARAFSQTKPRAAIAHQSYINTIFRYLSSSPSDEKLALLLQSVKWISMDSLK 174
             +E        F+ T P   +   S ++ +  Y+   P     A++   V  + +D+  
Sbjct: 101 AHDE---RTVSVFADTAPAIVLTASSVVDNVVEYVQPQPGQNAPAVI--EVDRLDLDA-- 160

Query: 175 QPHKEVEVNQNKHQPILYHSSSYYGCKPEETYLIQYTSGATAIPKPVVITAGAAAHNVRA 234
           +P        + H  ILY               +QYTSG+T  P  V+++      N   
Sbjct: 161 RPSSGSRSAAHGHPDILY---------------LQYTSGSTRTPAGVMVSNKNLFANFEQ 220

Query: 235 ARKAY------DLNPNDVIVSWLPQYHDCGLMF-LLLTVITGATCVLTSPISFVTRPTTW 294
              +Y         P   +VSWLP YHD G +  L+L ++ G   VLTSPI F+ RP  W
Sbjct: 221 IMTSYYGVYGKVAPPGSTVVSWLPFYHDMGFVLGLILPILAGIPAVLTSPIGFLQRPARW 280

Query: 295 LHLITAFKATCTPVPSFTLPLVLKRVKEETSSSRGLDLGSLRNLILINEPIYRSAVEEFV 354
           + ++ +     T  P+F   L  ++ K+E     GLDLG +  ++  +E +    ++ F+
Sbjct: 281 IQMLASNTLAFTAAPNFAFDLASRKTKDE--DMEGLDLGGVHGILNGSERVQPVTLKRFI 340

Query: 355 DVFKAVGLDPGCVSPSYGLAENCTFVSTAWCGGG------DRRWLFPAMASYRKLLPSAR 414
           D F    LDP  + PSYG+AE   +V+T   G        D + L    A   +      
Sbjct: 341 DRFAPFNLDPKAIRPSYGMAEATVYVATRKAGQPPKIVQFDPQKLPDGQAERTESDGGTP 400

Query: 415 LRDGSCTEIEVV-VVNGETGEVVEDGVEGEIWVSSPSNATAFDESYRYLEHPSLSRETFN 474
           L      + ++V +V+ +TG     G  GEIWV   + A        Y + P  +  TF+
Sbjct: 401 LVSYGIVDTQLVRIVDPDTGIERPAGTIGEIWVHGDNVAIG------YWQKPEATERTFS 460

Query: 475 SKINNKSSPN----FVRTGDRGVIKGADRFLFVIGRCSD-VIKFNNNQQEIHPHYIESIA 534
           + I N S       ++RTGD G +   +  LF++GR  D +I +  N     P  IE+  
Sbjct: 461 ATIVNPSEGTPAGPWLRTGDSGFLSEGE--LFIMGRIKDLLIVYGRNHS---PDDIEATI 520

Query: 535 YNNSSAYLRGGCIAAVKIS----ETIALVAEMQRDDKND---VELLKNICQEIRKALLIE 594
              S      G  AA+ +S    E +  + E+++ D++D    E L  + +E+  A+   
Sbjct: 521 QTISP-----GRCAAIAVSEHGAEKLVAIIELKKKDESDDEAAERLGFVKREVTSAISKS 566

BLAST of Lsi08G013910 vs. ExPASy Swiss-Prot
Match: P9WQ45 (Putative fatty-acid--CoA ligase fadD25 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=fadD25 PE=1 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 3.5e-43
Identity = 153/566 (27.03%), Postives = 253/566 (44.70%), Query Frame = 0

Query: 55  LTYRQLHDSVQCISDRLLRQLRRRDTVVVLCSAGLELVQLIYGCQRAGLVSVPISPPDPF 114
           LT+ QL+     ++ +L       D  ++L    L+ V       +AG+V+VP+S P   
Sbjct: 41  LTWSQLYRRTLNLAAQLREHGSTGDRALILAPQSLDYVVSFIASLQAGIVAVPLSIPQGG 100

Query: 115 LENENCQHLARAFSQTKPRAAIAHQSYINTIFRYLSSSPSDEKLALLLQSVKWISMDSLK 174
             +E        F+ T P   +   S ++ +  Y+   P     A++   V  + +D+  
Sbjct: 101 AHDE---RTVSVFADTAPAIVLTASSVVDNVVEYVQPQPGQNAPAVI--EVDRLDLDA-- 160

Query: 175 QPHKEVEVNQNKHQPILYHSSSYYGCKPEETYLIQYTSGATAIPKPVVITAGAAAHNVRA 234
           +P        + H  ILY               +QYTSG+T  P  V+++      N   
Sbjct: 161 RPSSGSRSAAHGHPDILY---------------LQYTSGSTRTPAGVMVSNKNLFANFEQ 220

Query: 235 ARKAY------DLNPNDVIVSWLPQYHDCGLMF-LLLTVITGATCVLTSPISFVTRPTTW 294
              +Y         P   +VSWLP YHD G +  L+L ++ G   VLTSPI F+ RP  W
Sbjct: 221 IMTSYYGVYGKVAPPGSTVVSWLPFYHDMGFVLGLILPILAGIPAVLTSPIGFLQRPARW 280

Query: 295 LHLITAFKATCTPVPSFTLPLVLKRVKEETSSSRGLDLGSLRNLILINEPIYRSAVEEFV 354
           + ++ +     T  P+F   L  ++ K+E     GLDLG +  ++  +E +    ++ F+
Sbjct: 281 IQMLASNTLAFTAAPNFAFDLASRKTKDE--DMEGLDLGGVHGILNGSERVQPVTLKRFI 340

Query: 355 DVFKAVGLDPGCVSPSYGLAENCTFVSTAWCGGG------DRRWLFPAMASYRKLLPSAR 414
           D F    LDP  + PSYG+AE   +V+T   G        D + L    A   +      
Sbjct: 341 DRFAPFNLDPKAIRPSYGMAEATVYVATRKAGQPPKIVQFDPQKLPDGQAERTESDGGTP 400

Query: 415 LRDGSCTEIEVV-VVNGETGEVVEDGVEGEIWVSSPSNATAFDESYRYLEHPSLSRETFN 474
           L      + ++V +V+ +TG     G  GEIWV   + A        Y + P  +  TF+
Sbjct: 401 LVSYGIVDTQLVRIVDPDTGIERPAGTIGEIWVHGDNVAIG------YWQKPEATERTFS 460

Query: 475 SKINNKSSPN----FVRTGDRGVIKGADRFLFVIGRCSD-VIKFNNNQQEIHPHYIESIA 534
           + I N S       ++RTGD G +   +  LF++GR  D +I +  N     P  IE+  
Sbjct: 461 ATIVNPSEGTPAGPWLRTGDSGFLSEGE--LFIMGRIKDLLIVYGRNHS---PDDIEATI 520

Query: 535 YNNSSAYLRGGCIAAVKIS----ETIALVAEMQRDDKND---VELLKNICQEIRKALLIE 594
              S      G  AA+ +S    E +  + E+++ D++D    E L  + +E+  A+   
Sbjct: 521 QTISP-----GRCAAIAVSEHGAEKLVAIIELKKKDESDDEAAERLGFVKREVTSAISKS 566

BLAST of Lsi08G013910 vs. ExPASy Swiss-Prot
Match: Q7TXM1 (Long-chain-fatty-acid--AMP ligase FadD26 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=fadD26 PE=1 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 5.1e-42
Identity = 162/619 (26.17%), Postives = 278/619 (44.91%), Query Frame = 0

Query: 17  PVVDRYLPVWASLPAFRSKPAFIWSE-DGTADAVNEGSFLTYRQLHDSVQCISDRLLRQL 76
           PV DR +P      A +   +  ++  D  +D       LT+ Q++     I++ L    
Sbjct: 2   PVTDRSVPSLLQERADQQPDSTAYTYIDYGSDPKGFADSLTWSQVYSRACIIAEELKLCG 61

Query: 77  RRRDTVVVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCQHLARAFSQTKPRAA 136
              D V VL   GLE V    G  +AG ++VP+S P   + ++    ++     +KP A 
Sbjct: 62  LPGDRVAVLAPQGLEYVLAFLGALQAGFIAVPLSTPQYGIHDD---RVSAVLQDSKPVAI 121

Query: 137 IAHQSYINTIFRYLSSSPSDEKLALLLQSVKWISMDSLKQPHKEVEVNQNKHQPILYHSS 196
           +   S +  + +Y +S   D + A ++  V  + +DS +Q    +     +H    Y   
Sbjct: 122 LTTSSVVGDVTKYAAS--HDGQPAPVVVEVDLLDLDSPRQ----MPAFSRQHTGAAY--- 181

Query: 197 SYYGCKPEETYLIQYTSGATAIPKPVVITAGAAAHNVRAARKAYDLNPNDV----IVSWL 256
                       +QYTSG+T  P  V+++      NV  +   Y  +P  +    +VSWL
Sbjct: 182 ------------LQYTSGSTRTPAGVIVSHTNVIANVTQSMYGYFGDPAKIPTGTVVSWL 241

Query: 257 PQYHDCGLMF-LLLTVITGATCVLTSPISFVTRPTTWLHLITAFKATCTPVPSFTLPLVL 316
           P YHD GL+  +   ++     VL SP+SF+ RP  W+ L+       +  P+F   L +
Sbjct: 242 PLYHDMGLILGICAPLVARRRAVLMSPMSFLRRPARWMQLLATSGRCFSAAPNFAFELAV 301

Query: 317 KRVKEETSSSRGLDLGSLRNLILINEPIYRSAVEEFVDVFKAVGLDPGCVSPSYGLAENC 376
           +R  ++  +  GLDL  +  ++  +E I+ + V  F++ F    L P  + PSYGLAE  
Sbjct: 302 RRTSDQDMA--GLDLRDVVGIVSGSERIHVATVRRFIERFAPYNLSPTAIRPSYGLAEAT 361

Query: 377 TFVSTAWCGGGDRRWLFPAMASYRKL-------------LPSARLRDGSCTEIEVVVVNG 436
            +V+    G   +   F     Y +L             + +  +  GS     V +VN 
Sbjct: 362 LYVAAPEAGAAPKTVRF----DYEQLTAGQARPCGTDGSVGTELISYGSPDPSSVRIVNP 421

Query: 437 ETGEVVEDGVEGEIWVSSPSNATAFDESYRYLEHPSLSRETFNSKINN--KSSPN--FVR 496
           ET      GV GEIWV           +  Y + P  + + F++K+ +   ++P   ++R
Sbjct: 422 ETMVENPPGVVGEIWVHGD------HVTMGYWQKPKQTAQVFDAKLVDPAPAAPEGPWLR 481

Query: 497 TGDRGVIKGADRFLFVIGRCSDVIKFNNNQQEIHPHYIESIAYNNSSAYLRGGCIAAV-- 556
           TGD GVI  +D  LF++GR  D++  +        HY + I    +   + GG  AA+  
Sbjct: 482 TGDLGVI--SDGELFIMGRIKDLLIVDGRN-----HYPDDI--EATIQEITGGRAAAIAV 541

Query: 557 --KISETIALVAEMQRDDKNDVEL---LKNICQEIRKALLIEEGIELGVVVLVKRGNVPK 606
              I+E +  + E +R      E+   L+++ +E+  A+     + +  +VLV  G++P 
Sbjct: 542 PDDITEQLVAIIEFKRRGSTAEEVMLKLRSVKREVTSAISKSHSLRVADLVLVSPGSIPI 575

BLAST of Lsi08G013910 vs. ExPASy TrEMBL
Match: A0A0A0LL23 (AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G361690 PE=4 SV=1)

HSP 1 Score: 1037.3 bits (2681), Expect = 2.6e-299
Identity = 524/637 (82.26%), Postives = 569/637 (89.32%), Query Frame = 0

Query: 4   MLYENFDPLFPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTADAVNEGSFLTYRQLHDS 63
           MLYENFDPLFP+QPVVDRYLPVWASLPAF+SKPAFIWSEDGTA A+NE SFLTYRQLHDS
Sbjct: 1   MLYENFDPLFPEQPVVDRYLPVWASLPAFQSKPAFIWSEDGTAKAINEDSFLTYRQLHDS 60

Query: 64  VQCISDRLLRQLRRRDTVVVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCQHL 123
           VQ I+++LLRQLRRRDTVVVLCSAGL+ VQLIYGCQRAGLVSVPISPPD F ENENC HL
Sbjct: 61  VQLITEQLLRQLRRRDTVVVLCSAGLDFVQLIYGCQRAGLVSVPISPPDVFSENENCHHL 120

Query: 124 ARAFSQTKPRAAIAHQSYINTIFRYLSSSPSDEKLALLLQSVKWISMDSLKQPHKEVEVN 183
           ARA SQTKPRAAIAHQSYINT+FRY+SSS +D+KLALLL+ V+WISM+SLKQPHKE E+N
Sbjct: 121 ARALSQTKPRAAIAHQSYINTVFRYISSS-ADKKLALLLKGVRWISMESLKQPHKESELN 180

Query: 184 QNKHQPILYHSSSYYGCKPEETYLIQYTSGATAIPKPVVITAGAAAHNVRAARKAYDLNP 243
           Q+KHQ   YHSSSY GC P++ YLIQYTSGATAI K VVITAGAAAHNVRAARKAYDLNP
Sbjct: 181 QHKHQSSFYHSSSYSGCNPDDPYLIQYTSGATAISKAVVITAGAAAHNVRAARKAYDLNP 240

Query: 244 NDVIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTRPTTWLHLITAFKATCTPVPS 303
           ND+IVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVT+P TWLHLITAFKATCTPVPS
Sbjct: 241 NDLIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTQPITWLHLITAFKATCTPVPS 300

Query: 304 FTLPLVLKRVKEETSSSRGLDLGSLRNLILINEPIYRSAVEEFVDVFKAVGLDPGCVSPS 363
           FTLPLVLKRV   +  +  LDL SLRNLILINEPIYRS VEEFV+VFK VGLDPGCVSPS
Sbjct: 301 FTLPLVLKRVGYSSGRAGNLDLRSLRNLILINEPIYRSTVEEFVEVFKTVGLDPGCVSPS 360

Query: 364 YGLAENCTFVSTAWCGGGDRRWLFPAMASYRKLLPSARLRDGSCTEIEVVVVNGETGEVV 423
           YGLAENCTFVSTAWCGGG     FPAM SYR+LLPS RLRDG C EIEVVVVN ETGEVV
Sbjct: 361 YGLAENCTFVSTAWCGGGG----FPAMPSYRQLLPSGRLRDGMCKEIEVVVVNEETGEVV 420

Query: 424 EDGVEGEIWVSSPSNATAFDESYRYLEHPSLSRETFNSKINNKSSPNFVRTGDRGVIKGA 483
           EDGVEGEIWVSSPSNA        YL HPS++ ETF+SK+ NKSS NFVRTGDRGVIKG+
Sbjct: 421 EDGVEGEIWVSSPSNALG------YLGHPSVTEETFHSKLKNKSSLNFVRTGDRGVIKGS 480

Query: 484 DRFLFVIGRCSDVIKFNNNQQEIHPHYIESIAYNNSSAYLRGGCIAAVKISETIALVAEM 543
           DRFLFVIGRCSDVIK NNN Q+IHPHYIES +YNN SAYLRGGCIAAVK+S TIALVAEM
Sbjct: 481 DRFLFVIGRCSDVIKLNNNNQQIHPHYIESTSYNNFSAYLRGGCIAAVKVSGTIALVAEM 540

Query: 544 QRDDKNDVELLKNICQEIRKALLIEEGIELGVVVLVKRGNVPKTTSGKVKRWAVKEKLAG 603
           QRDD++D ELL+ IC++IRKA+LIEEGIELG+VVLVKRGNV KTTSGKVKRW VKEKLAG
Sbjct: 541 QRDDRHDAELLRKICEDIRKAVLIEEGIELGLVVLVKRGNVSKTTSGKVKRWVVKEKLAG 600

Query: 604 GGLGVLMAVKFEKNCDVLMDLERKEEFRTRPLLLSLL 641
           GGLGVLMA +F KNC   +DL+RK EF TRP+L+S+L
Sbjct: 601 GGLGVLMAFEFGKNC---VDLKRKGEFETRPVLISML 623

BLAST of Lsi08G013910 vs. ExPASy TrEMBL
Match: A0A6J1HF51 (uncharacterized protein LOC111462961 OS=Cucurbita moschata OX=3662 GN=LOC111462961 PE=4 SV=1)

HSP 1 Score: 1014.2 bits (2621), Expect = 2.4e-292
Identity = 521/647 (80.53%), Postives = 562/647 (86.86%), Query Frame = 0

Query: 2   KIMLYENFDPLFPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTADAVNEGSFLTYRQLH 61
           +IM YENFDP+FPDQPVVD YLPVWASLPAFRSKPAFIWSEDGTAD +NEGSFLTY QLH
Sbjct: 12  EIMSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLH 71

Query: 62  DSVQCISDRLLRQLRRRDTVVVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCQ 121
           DSVQ ISD L+RQ+RRRDTVV+LCS GL+LVQLIYGCQRAGLVSVPISPPDP  ENENC 
Sbjct: 72  DSVQFISDELIRQVRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPRFENENCH 131

Query: 122 HLARAFSQTKPRAAIAHQSYINTIFRYLSSSPSDEKLALLLQSVKWISMDSLK-----QP 181
           HLARA SQTKPRAAIAHQ+YI ++FRYLS SP+D KLALLLQSV+WISM++L+     + 
Sbjct: 132 HLARALSQTKPRAAIAHQAYIASVFRYLSLSPTDGKLALLLQSVQWISMETLRTCTAAEA 191

Query: 182 HKEVEVNQNKHQPILYHS-SSYYGCKPEETYLIQYTSGATAIPKPVVITAGAAAHNVRAA 241
            + V V  +K QP  YHS SSYYGCKPEE YLIQYTSGAT IPKPVV+TAGAAAHNVRAA
Sbjct: 192 DQVVSVISSKDQPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRAA 251

Query: 242 RKAYDLNPNDVIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTRPTTWLHLITAFK 301
           RKAYDLNPNDVIVSWLPQYHDCGLMFLLLTV++GATCVLTSPISFVTRP TWLHLIT FK
Sbjct: 252 RKAYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFK 311

Query: 302 ATCTPVPSFTLPLVLKRVKEETSSSRGLDLGSLRNLILINEPIYRSAVEEFVDVFKAVGL 361
           ATCTPVPSFTLPLVLKRVKEET    GLDL SLRNLILINEP+YRS VEEFVDVFKAVGL
Sbjct: 312 ATCTPVPSFTLPLVLKRVKEETPPCGGLDLCSLRNLILINEPVYRSGVEEFVDVFKAVGL 371

Query: 362 DPGCVSPSYGLAENCTFVSTAWCG--GGDRRWLFPAMASYRKLLPSARLRDGSCTEIEVV 421
           +PGCVSPSYGLAENCTFVSTAW G  GG RRW FPAM SYRKLLPSARL D S +EIEVV
Sbjct: 372 NPGCVSPSYGLAENCTFVSTAWSGGEGGRRRW-FPAMPSYRKLLPSARLMDESSSEIEVV 431

Query: 422 VVNGETGEVVEDGVEGEIWVSSPSNATAFDESYRYLEHPSLSRETFNSKINNKSSPNFVR 481
           VVNGETGE+VEDGVEGEIW+SSPSNA+       YL HPSL+R+TF+ K++NKSS  FVR
Sbjct: 432 VVNGETGEIVEDGVEGEIWISSPSNASG------YLGHPSLTRDTFHCKLSNKSSHKFVR 491

Query: 482 TGDRGVIKGADRFLFVIGRCSDVIKFNNNQQEIHPHYIESIAYNNSSAYLRGGCIAAVKI 541
           TGDRGVIKG DRFLFVIGRCSDVI   NN QEIHPHYIESIAYNN S+YLRGGC+AA+KI
Sbjct: 492 TGDRGVIKGTDRFLFVIGRCSDVITLTNN-QEIHPHYIESIAYNNCSSYLRGGCLAAIKI 551

Query: 542 SETIALVAEMQRDDKNDVELLKNICQEIRKALLIEEGIELGVVVLVKRGNVPKTTSGKVK 601
           S+TIA+VAEMQR DKND E L+ IC+ IRKA LIEEGIELG+VVLVKRGNVPKTTSGKVK
Sbjct: 552 SDTIAVVAEMQRQDKNDAESLRKICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVK 611

Query: 602 RWAVKEKLAGGGLGVLMAVKFEKNCDVLMDLERKEEFRTRPLLLSLL 641
           RWA KEKLAGGG+ VLMAVKF K C V  DLE       RPLLLSLL
Sbjct: 612 RWAAKEKLAGGGMSVLMAVKFGKTCGVTKDLE-----TNRPLLLSLL 645

BLAST of Lsi08G013910 vs. ExPASy TrEMBL
Match: A0A6J1K7R6 (uncharacterized protein LOC111492429 OS=Cucurbita maxima OX=3661 GN=LOC111492429 PE=4 SV=1)

HSP 1 Score: 984.2 bits (2543), Expect = 2.6e-283
Identity = 506/644 (78.57%), Postives = 550/644 (85.40%), Query Frame = 0

Query: 4   MLYENFDPLFPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTADAVNEGSFLTYRQLHDS 63
           M YENFDP+FPDQPVVD YLPVWASLPAFRSKPAFIWSEDGTAD +NEGSFLTY QLHDS
Sbjct: 1   MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 60

Query: 64  VQCISDRLLRQLRRRDTVVVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCQHL 123
           VQ IS++L+RQ+RRRDTV+VLCS GL+LVQLIYGCQRAGLVSVPISPPDP  ENENC H 
Sbjct: 61  VQFISEQLIRQVRRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFENENCHHF 120

Query: 124 ARAFSQTKPRAAIAHQSYINTIFRYLSSSPSDEKLALLLQSVKWISMDSLK-----QPHK 183
           ARA SQTKPRA IAH+ YI ++FRYLSSS  D KLALLLQSV+WISM++L+     +  +
Sbjct: 121 ARALSQTKPRAVIAHEPYIVSVFRYLSSSQIDGKLALLLQSVQWISMETLRTCTAAEADQ 180

Query: 184 EVEVNQNKHQPILYHS-SSYYGCKPEETYLIQYTSGATAIPKPVVITAGAAAHNVRAARK 243
            V V  +K +P  YHS SSYYGCKPEE YLIQYTSGAT IPKPVV+TAGAAAHNVRAARK
Sbjct: 181 VVSVISSKDRPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRAARK 240

Query: 244 AYDLNPNDVIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTRPTTWLHLITAFKAT 303
           AYDLNPNDVIVSWLPQYHDCGLMFLLLTV++GATCVLTSPISFVTRP TWLHLIT FKAT
Sbjct: 241 AYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKAT 300

Query: 304 CTPVPSFTLPLVLKRVKEETSSSRGLDLGSLRNLILINEPIYRSAVEEFVDVFKAVGLDP 363
           CTPVPSFTLPLVLKRVKEET     LDL  LRNLILINEP+YRS VEEFVDVFKAVGL+P
Sbjct: 301 CTPVPSFTLPLVLKRVKEETPPGGDLDLCCLRNLILINEPVYRSGVEEFVDVFKAVGLNP 360

Query: 364 GCVSPSYGLAENCTFVSTAWCGG-GDRRWLFPAMASYRKLLPSARLRDGSCTEIEVVVVN 423
           GCVSPSYGLAEN TFVSTAW GG G  R  FPAM SYRKLLP ARL DGS  EIEVVVVN
Sbjct: 361 GCVSPSYGLAENSTFVSTAWSGGEGGLRRCFPAMPSYRKLLPCARLTDGSSAEIEVVVVN 420

Query: 424 GETGEVVEDGVEGEIWVSSPSNATAFDESYRYLEHPSLSRETFNSKINNKSSPNFVRTGD 483
           GETGE+V+DGVEGEIW+SSPSNA+       YL HP L+R+TF+ K++NKSS  FVRTGD
Sbjct: 421 GETGEMVDDGVEGEIWISSPSNASG------YLGHPLLTRDTFHCKLSNKSSHKFVRTGD 480

Query: 484 RGVIKGADRFLFVIGRCSDVIKFNNNQQEIHPHYIESIAYNNSSAYLRGGCIAAVKISET 543
           RGVIKGADRFLFVIGRCSDVI   NN QEIHPHYIESIAYNN SAYLRGGC+AA+K+S+T
Sbjct: 481 RGVIKGADRFLFVIGRCSDVITLTNN-QEIHPHYIESIAYNNFSAYLRGGCLAAIKVSDT 540

Query: 544 IALVAEMQRDDKNDVELLKNICQEIRKALLIEEGIELGVVVLVKRGNVPKTTSGKVKRWA 603
           IA+VAEMQR+DKND E L+ IC+ IRKA LIEEGIELG+VVLVKRGNVPKTTSGKVKRW 
Sbjct: 541 IAVVAEMQREDKNDAETLRQICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWL 600

Query: 604 VKEKLAGGGLGVLMAVKFEKNCDVLMDLERKEEFRTRPLLLSLL 641
            KEKLAGGG+ VLMAVKF   C V  DL+       RPLLLSLL
Sbjct: 601 AKEKLAGGGMSVLMAVKFGTICGVTKDLD-----GNRPLLLSLL 632

BLAST of Lsi08G013910 vs. ExPASy TrEMBL
Match: A0A5A7VDG2 (Long-chain-fatty-acid--AMP ligase FadD26-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005700 PE=4 SV=1)

HSP 1 Score: 788.5 bits (2035), Expect = 2.1e-224
Identity = 405/498 (81.33%), Postives = 439/498 (88.15%), Query Frame = 0

Query: 145 IFRYLSSSPSDEKLALLLQSVKWISMDSLKQPHKEVEVNQNKHQPILYHSSSYYGCKPEE 204
           I+RY+SSSP+DEKLALLL+SV+WISM+SLK+PHKE E+NQ+KH    +HSSSY GC P++
Sbjct: 11  IYRYISSSPADEKLALLLKSVQWISMESLKEPHKESELNQHKHHSFSHHSSSYSGCNPDD 70

Query: 205 TYLIQYTSGATAIPKPVVITAGAAAHNVRAARKAYDLNPNDVIVSWLPQYHDCGLMFLLL 264
            YLIQYTSGATAI KPVVITAGAAAHNVRAARKAYDLNPNDVIVSWLPQ+HDCGLMFLLL
Sbjct: 71  PYLIQYTSGATAISKPVVITAGAAAHNVRAARKAYDLNPNDVIVSWLPQFHDCGLMFLLL 130

Query: 265 TVITGATCVLTSPISFVTRPTTWLHLITAFKATCTPVPSFTLPLVLKRVKEETSSSRG-L 324
           TVITGATCVLTSPISFVT+P TWLHLITAFKATCTPVPSFTLPLVLKRV   +S   G L
Sbjct: 131 TVITGATCVLTSPISFVTQPITWLHLITAFKATCTPVPSFTLPLVLKRVGNYSSGRAGNL 190

Query: 325 DLGSLRNLILINEPIYRSAVEEFVDVFKAVGLDPGCVSPSYGLAENCTFVSTAWCGGGDR 384
           DL SLRNLILINEPIYRS VEEFVDVFKAVGLDPGCVSPSYGLAENCTFVSTAWCGGG  
Sbjct: 191 DLRSLRNLILINEPIYRSTVEEFVDVFKAVGLDPGCVSPSYGLAENCTFVSTAWCGGGG- 250

Query: 385 RWLFPAMASYRKLLPSARLRDGSCTEIEVVVVNGETGEVVEDGVEGEIWVSSPSNATAFD 444
              FPAM SYRKLLPS RLRDG C EIEVVVVN ETGEVVEDGVEGEIW+SSPSNA    
Sbjct: 251 ---FPAMPSYRKLLPSGRLRDGMCKEIEVVVVNEETGEVVEDGVEGEIWISSPSNALG-- 310

Query: 445 ESYRYLEHPSLSRETFNSKINNKSSPNFVRTGDRGVIKGADRFLFVIGRCSDVIKFNNNQ 504
               YL HPSL+ ETF+ K+ NK+S NFVRTGDRGVIKG+DRFLFVIGRCSDVIKFN+N 
Sbjct: 311 ----YLGHPSLTEETFHRKLKNKASLNFVRTGDRGVIKGSDRFLFVIGRCSDVIKFNDNN 370

Query: 505 QEIHPHYIESIAYNNSSAYLRGGCIAAVKISE-TIALVAEMQRDDKNDVELLKNICQEIR 564
           Q+IHPHYIES +Y N SAYLRGGCIAAVKIS  T+ALVAEMQRDD+ND ELL+ IC+EIR
Sbjct: 371 QQIHPHYIESTSYKNFSAYLRGGCIAAVKISSGTVALVAEMQRDDRNDAELLRKICEEIR 430

Query: 565 KALLIEEGIELGVVVLVKRGNVPKTTSGKVKRWAVKEKLAGGGLGVLMAVKFEKNCDVLM 624
           KA+LIEEGIELGVVVLVKRGNV KTTSGKVKRW VKEKL GGGL VLMAV+F K+C+ L 
Sbjct: 431 KAVLIEEGIELGVVVLVKRGNVSKTTSGKVKRWVVKEKLGGGGLEVLMAVEFGKDCEGLK 490

Query: 625 DLERKEEFRTRPLLLSLL 641
           DLERK EF TRP+LLS+L
Sbjct: 491 DLERKGEFGTRPVLLSML 498

BLAST of Lsi08G013910 vs. ExPASy TrEMBL
Match: A0A1S3BAI6 (long-chain-fatty-acid--AMP ligase FadD26-like OS=Cucumis melo OX=3656 GN=LOC103487973 PE=4 SV=1)

HSP 1 Score: 748.0 bits (1930), Expect = 3.2e-212
Identity = 385/473 (81.40%), Postives = 414/473 (87.53%), Query Frame = 0

Query: 170 MDSLKQPHKEVEVNQNKHQPILYHSSSYYGCKPEETYLIQYTSGATAIPKPVVITAGAAA 229
           M+SLK+PHKE E+NQ+KH    +HSSSY GC P++ YLIQYTSGATAI KPVVITAGAAA
Sbjct: 1   MESLKEPHKESELNQHKHHSFSHHSSSYSGCNPDDPYLIQYTSGATAISKPVVITAGAAA 60

Query: 230 HNVRAARKAYDLNPNDVIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTRPTTWLH 289
           HNVRAARKAYDLNPNDVIVSWLPQ+HDCGLMFLLLTVITGATCVLTSPISFVT+P TWLH
Sbjct: 61  HNVRAARKAYDLNPNDVIVSWLPQFHDCGLMFLLLTVITGATCVLTSPISFVTQPITWLH 120

Query: 290 LITAFKATCTPVPSFTLPLVLKRVKEETSSSRG-LDLGSLRNLILINEPIYRSAVEEFVD 349
           LITAFKATCTPVPSFTLPLVLKRV   +S   G LDL SLRNLILINEPIYRS VEEFVD
Sbjct: 121 LITAFKATCTPVPSFTLPLVLKRVGNYSSGRAGNLDLRSLRNLILINEPIYRSTVEEFVD 180

Query: 350 VFKAVGLDPGCVSPSYGLAENCTFVSTAWCGGGDRRWLFPAMASYRKLLPSARLRDGSCT 409
           VFKAVGLDPGCVSPSYGLAENCTFVSTAWCGGG     FPAM SYRKLLPS RLRDG C 
Sbjct: 181 VFKAVGLDPGCVSPSYGLAENCTFVSTAWCGGGG----FPAMPSYRKLLPSGRLRDGMCK 240

Query: 410 EIEVVVVNGETGEVVEDGVEGEIWVSSPSNATAFDESYRYLEHPSLSRETFNSKINNKSS 469
           EIEVVVVN ETGEVVEDGVEGEIW+SSPSNA        YL HPSL+ ETF+ K+ NK+S
Sbjct: 241 EIEVVVVNEETGEVVEDGVEGEIWISSPSNALG------YLGHPSLTEETFHRKLKNKAS 300

Query: 470 PNFVRTGDRGVIKGADRFLFVIGRCSDVIKFNNNQQEIHPHYIESIAYNNSSAYLRGGCI 529
            NFVRTGDRGVIKG+DRFLFVIGRCSDVIKFN+N Q+IHPHYIES +Y N SAYLRGGCI
Sbjct: 301 LNFVRTGDRGVIKGSDRFLFVIGRCSDVIKFNDNNQQIHPHYIESTSYKNFSAYLRGGCI 360

Query: 530 AAVKISE-TIALVAEMQRDDKNDVELLKNICQEIRKALLIEEGIELGVVVLVKRGNVPKT 589
           AAVKIS  T+ALVAEMQRDD+ND ELL+ IC+EIRKA+LIEEGIELGVVVLVKRGNV KT
Sbjct: 361 AAVKISSGTVALVAEMQRDDRNDAELLRKICEEIRKAVLIEEGIELGVVVLVKRGNVSKT 420

Query: 590 TSGKVKRWAVKEKLAGGGLGVLMAVKFEKNCDVLMDLERKEEFRTRPLLLSLL 641
           TSGKVKRW VKEKL GGGL VLMAV+F K+C+ L DLERK EF TRP+LLS+L
Sbjct: 421 TSGKVKRWVVKEKLGGGGLEVLMAVEFGKDCEGLKDLERKGEFGTRPVLLSML 463

BLAST of Lsi08G013910 vs. NCBI nr
Match: XP_038884878.1 (long-chain-fatty-acid--AMP ligase FadD26-like [Benincasa hispida])

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 556/637 (87.28%), Postives = 593/637 (93.09%), Query Frame = 0

Query: 4   MLYENFDPLFPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTADAVNEGSFLTYRQLHDS 63
           MLYENFDPLFPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTA ++N+GSFLTY+QLHDS
Sbjct: 1   MLYENFDPLFPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTAGSLNDGSFLTYQQLHDS 60

Query: 64  VQCISDRLLRQLRRRDTVVVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCQHL 123
           VQ ISD+LLRQLRRRDTV VLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENC HL
Sbjct: 61  VQFISDQLLRQLRRRDTVAVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCHHL 120

Query: 124 ARAFSQTKPRAAIAHQSYINTIFRYLSSSPSDEKLALLLQSVKWISMDSLKQPHKEVEVN 183
           ARA SQTKPRAAIAHQSYI TIFRYLSSSP+DEKLALLLQSV+WISMDSLKQP K+ EVN
Sbjct: 121 ARALSQTKPRAAIAHQSYIYTIFRYLSSSPTDEKLALLLQSVQWISMDSLKQPDKKAEVN 180

Query: 184 QNKHQPILYHSSSYYGCKPEETYLIQYTSGATAIPKPVVITAGAAAHNVRAARKAYDLNP 243
           Q+KHQPILYHSSSYYGC P+E YLIQYTSGATAIPKPVV+TAGAAAHNVR ARKAY+LNP
Sbjct: 181 QDKHQPILYHSSSYYGCNPKEPYLIQYTSGATAIPKPVVVTAGAAAHNVRVARKAYNLNP 240

Query: 244 NDVIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTRPTTWLHLITAFKATCTPVPS 303
           NDVIVSWLPQYHDCGLMFLLL+VITGATCVLTSP+SFVT P  WLHLITAFKATCTPVPS
Sbjct: 241 NDVIVSWLPQYHDCGLMFLLLSVITGATCVLTSPMSFVTNPIRWLHLITAFKATCTPVPS 300

Query: 304 FTLPLVLKRVKEETSSSRGLDLGSLRNLILINEPIYRSAVEEFVDVFKAVGLDPGCVSPS 363
           FTLPLVLKRVK+ET SS+GLDL SLRNLILINEPIYR+AVEEFVD FKAVGLDPGCVSPS
Sbjct: 301 FTLPLVLKRVKDETPSSKGLDLRSLRNLILINEPIYRAAVEEFVDEFKAVGLDPGCVSPS 360

Query: 364 YGLAENCTFVSTAWCGGGDRRWLFPAMASYRKLLPSARLRDGSCTEIEVVVVNGETGEVV 423
           YGLAENCTFVSTAW GG  RRW FPAM +YRKLLPS RLRDG CTEI+VVVVNGETGEVV
Sbjct: 361 YGLAENCTFVSTAWSGG--RRWWFPAMPNYRKLLPSGRLRDGLCTEIKVVVVNGETGEVV 420

Query: 424 EDGVEGEIWVSSPSNATAFDESYRYLEHPSLSRETFNSKINNKSSPNFVRTGDRGVIKGA 483
           EDGVEGEIWVSSPSNA+       YL HPSL+++TF+SKINNKSSPNF+RTGDRGVI+GA
Sbjct: 421 EDGVEGEIWVSSPSNASG------YLGHPSLTKKTFHSKINNKSSPNFIRTGDRGVIRGA 480

Query: 484 DRFLFVIGRCSDVIKFNNNQQEIHPHYIESIAYNNSSAYLRGGCIAAVKISETIALVAEM 543
           DRFLFVIGRCSD+IKFNN QQEIHPHYIES AYNNSSAYLRGGCIAAVKISETIA+VAEM
Sbjct: 481 DRFLFVIGRCSDLIKFNNTQQEIHPHYIESTAYNNSSAYLRGGCIAAVKISETIAVVAEM 540

Query: 544 QRDDKNDVELLKNICQEIRKALLIEEGIELGVVVLVKRGNVPKTTSGKVKRWAVKEKLAG 603
           QRDDKND ELL+ IC+EIRKA+ IEEG ELG+VVLVKRGN+PKTTSGKVKRWAVKEKLAG
Sbjct: 541 QRDDKNDAELLRKICEEIRKAVSIEEGTELGLVVLVKRGNIPKTTSGKVKRWAVKEKLAG 600

Query: 604 GGLGVLMAVKFEKNCDVLMDLERKEEFRTRPLLLSLL 641
           GGLGVLMA+ FEKNC+V   L+R+EE RTRP LLSLL
Sbjct: 601 GGLGVLMAIGFEKNCEV---LQREEELRTRPALLSLL 626

BLAST of Lsi08G013910 vs. NCBI nr
Match: XP_011649625.1 (uncharacterized protein LOC105434645 [Cucumis sativus] >KGN62605.1 hypothetical protein Csa_021877 [Cucumis sativus])

HSP 1 Score: 1037.3 bits (2681), Expect = 5.4e-299
Identity = 524/637 (82.26%), Postives = 569/637 (89.32%), Query Frame = 0

Query: 4   MLYENFDPLFPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTADAVNEGSFLTYRQLHDS 63
           MLYENFDPLFP+QPVVDRYLPVWASLPAF+SKPAFIWSEDGTA A+NE SFLTYRQLHDS
Sbjct: 1   MLYENFDPLFPEQPVVDRYLPVWASLPAFQSKPAFIWSEDGTAKAINEDSFLTYRQLHDS 60

Query: 64  VQCISDRLLRQLRRRDTVVVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCQHL 123
           VQ I+++LLRQLRRRDTVVVLCSAGL+ VQLIYGCQRAGLVSVPISPPD F ENENC HL
Sbjct: 61  VQLITEQLLRQLRRRDTVVVLCSAGLDFVQLIYGCQRAGLVSVPISPPDVFSENENCHHL 120

Query: 124 ARAFSQTKPRAAIAHQSYINTIFRYLSSSPSDEKLALLLQSVKWISMDSLKQPHKEVEVN 183
           ARA SQTKPRAAIAHQSYINT+FRY+SSS +D+KLALLL+ V+WISM+SLKQPHKE E+N
Sbjct: 121 ARALSQTKPRAAIAHQSYINTVFRYISSS-ADKKLALLLKGVRWISMESLKQPHKESELN 180

Query: 184 QNKHQPILYHSSSYYGCKPEETYLIQYTSGATAIPKPVVITAGAAAHNVRAARKAYDLNP 243
           Q+KHQ   YHSSSY GC P++ YLIQYTSGATAI K VVITAGAAAHNVRAARKAYDLNP
Sbjct: 181 QHKHQSSFYHSSSYSGCNPDDPYLIQYTSGATAISKAVVITAGAAAHNVRAARKAYDLNP 240

Query: 244 NDVIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTRPTTWLHLITAFKATCTPVPS 303
           ND+IVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVT+P TWLHLITAFKATCTPVPS
Sbjct: 241 NDLIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTQPITWLHLITAFKATCTPVPS 300

Query: 304 FTLPLVLKRVKEETSSSRGLDLGSLRNLILINEPIYRSAVEEFVDVFKAVGLDPGCVSPS 363
           FTLPLVLKRV   +  +  LDL SLRNLILINEPIYRS VEEFV+VFK VGLDPGCVSPS
Sbjct: 301 FTLPLVLKRVGYSSGRAGNLDLRSLRNLILINEPIYRSTVEEFVEVFKTVGLDPGCVSPS 360

Query: 364 YGLAENCTFVSTAWCGGGDRRWLFPAMASYRKLLPSARLRDGSCTEIEVVVVNGETGEVV 423
           YGLAENCTFVSTAWCGGG     FPAM SYR+LLPS RLRDG C EIEVVVVN ETGEVV
Sbjct: 361 YGLAENCTFVSTAWCGGGG----FPAMPSYRQLLPSGRLRDGMCKEIEVVVVNEETGEVV 420

Query: 424 EDGVEGEIWVSSPSNATAFDESYRYLEHPSLSRETFNSKINNKSSPNFVRTGDRGVIKGA 483
           EDGVEGEIWVSSPSNA        YL HPS++ ETF+SK+ NKSS NFVRTGDRGVIKG+
Sbjct: 421 EDGVEGEIWVSSPSNALG------YLGHPSVTEETFHSKLKNKSSLNFVRTGDRGVIKGS 480

Query: 484 DRFLFVIGRCSDVIKFNNNQQEIHPHYIESIAYNNSSAYLRGGCIAAVKISETIALVAEM 543
           DRFLFVIGRCSDVIK NNN Q+IHPHYIES +YNN SAYLRGGCIAAVK+S TIALVAEM
Sbjct: 481 DRFLFVIGRCSDVIKLNNNNQQIHPHYIESTSYNNFSAYLRGGCIAAVKVSGTIALVAEM 540

Query: 544 QRDDKNDVELLKNICQEIRKALLIEEGIELGVVVLVKRGNVPKTTSGKVKRWAVKEKLAG 603
           QRDD++D ELL+ IC++IRKA+LIEEGIELG+VVLVKRGNV KTTSGKVKRW VKEKLAG
Sbjct: 541 QRDDRHDAELLRKICEDIRKAVLIEEGIELGLVVLVKRGNVSKTTSGKVKRWVVKEKLAG 600

Query: 604 GGLGVLMAVKFEKNCDVLMDLERKEEFRTRPLLLSLL 641
           GGLGVLMA +F KNC   +DL+RK EF TRP+L+S+L
Sbjct: 601 GGLGVLMAFEFGKNC---VDLKRKGEFETRPVLISML 623

BLAST of Lsi08G013910 vs. NCBI nr
Match: XP_022962563.1 (uncharacterized protein LOC111462961 [Cucurbita moschata])

HSP 1 Score: 1014.2 bits (2621), Expect = 4.9e-292
Identity = 521/647 (80.53%), Postives = 562/647 (86.86%), Query Frame = 0

Query: 2   KIMLYENFDPLFPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTADAVNEGSFLTYRQLH 61
           +IM YENFDP+FPDQPVVD YLPVWASLPAFRSKPAFIWSEDGTAD +NEGSFLTY QLH
Sbjct: 12  EIMSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLH 71

Query: 62  DSVQCISDRLLRQLRRRDTVVVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCQ 121
           DSVQ ISD L+RQ+RRRDTVV+LCS GL+LVQLIYGCQRAGLVSVPISPPDP  ENENC 
Sbjct: 72  DSVQFISDELIRQVRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPRFENENCH 131

Query: 122 HLARAFSQTKPRAAIAHQSYINTIFRYLSSSPSDEKLALLLQSVKWISMDSLK-----QP 181
           HLARA SQTKPRAAIAHQ+YI ++FRYLS SP+D KLALLLQSV+WISM++L+     + 
Sbjct: 132 HLARALSQTKPRAAIAHQAYIASVFRYLSLSPTDGKLALLLQSVQWISMETLRTCTAAEA 191

Query: 182 HKEVEVNQNKHQPILYHS-SSYYGCKPEETYLIQYTSGATAIPKPVVITAGAAAHNVRAA 241
            + V V  +K QP  YHS SSYYGCKPEE YLIQYTSGAT IPKPVV+TAGAAAHNVRAA
Sbjct: 192 DQVVSVISSKDQPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRAA 251

Query: 242 RKAYDLNPNDVIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTRPTTWLHLITAFK 301
           RKAYDLNPNDVIVSWLPQYHDCGLMFLLLTV++GATCVLTSPISFVTRP TWLHLIT FK
Sbjct: 252 RKAYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFK 311

Query: 302 ATCTPVPSFTLPLVLKRVKEETSSSRGLDLGSLRNLILINEPIYRSAVEEFVDVFKAVGL 361
           ATCTPVPSFTLPLVLKRVKEET    GLDL SLRNLILINEP+YRS VEEFVDVFKAVGL
Sbjct: 312 ATCTPVPSFTLPLVLKRVKEETPPCGGLDLCSLRNLILINEPVYRSGVEEFVDVFKAVGL 371

Query: 362 DPGCVSPSYGLAENCTFVSTAWCG--GGDRRWLFPAMASYRKLLPSARLRDGSCTEIEVV 421
           +PGCVSPSYGLAENCTFVSTAW G  GG RRW FPAM SYRKLLPSARL D S +EIEVV
Sbjct: 372 NPGCVSPSYGLAENCTFVSTAWSGGEGGRRRW-FPAMPSYRKLLPSARLMDESSSEIEVV 431

Query: 422 VVNGETGEVVEDGVEGEIWVSSPSNATAFDESYRYLEHPSLSRETFNSKINNKSSPNFVR 481
           VVNGETGE+VEDGVEGEIW+SSPSNA+       YL HPSL+R+TF+ K++NKSS  FVR
Sbjct: 432 VVNGETGEIVEDGVEGEIWISSPSNASG------YLGHPSLTRDTFHCKLSNKSSHKFVR 491

Query: 482 TGDRGVIKGADRFLFVIGRCSDVIKFNNNQQEIHPHYIESIAYNNSSAYLRGGCIAAVKI 541
           TGDRGVIKG DRFLFVIGRCSDVI   NN QEIHPHYIESIAYNN S+YLRGGC+AA+KI
Sbjct: 492 TGDRGVIKGTDRFLFVIGRCSDVITLTNN-QEIHPHYIESIAYNNCSSYLRGGCLAAIKI 551

Query: 542 SETIALVAEMQRDDKNDVELLKNICQEIRKALLIEEGIELGVVVLVKRGNVPKTTSGKVK 601
           S+TIA+VAEMQR DKND E L+ IC+ IRKA LIEEGIELG+VVLVKRGNVPKTTSGKVK
Sbjct: 552 SDTIAVVAEMQRQDKNDAESLRKICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVK 611

Query: 602 RWAVKEKLAGGGLGVLMAVKFEKNCDVLMDLERKEEFRTRPLLLSLL 641
           RWA KEKLAGGG+ VLMAVKF K C V  DLE       RPLLLSLL
Sbjct: 612 RWAAKEKLAGGGMSVLMAVKFGKTCGVTKDLE-----TNRPLLLSLL 645

BLAST of Lsi08G013910 vs. NCBI nr
Match: XP_023546350.1 (uncharacterized protein LOC111805474 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1006.1 bits (2600), Expect = 1.3e-289
Identity = 515/647 (79.60%), Postives = 560/647 (86.55%), Query Frame = 0

Query: 2   KIMLYENFDPLFPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTADAVNEGSFLTYRQLH 61
           +IM YENFDP+FPDQPVVD YLPVWASLPAFRSKPAFIWSEDGTAD +NEGSFLTY QLH
Sbjct: 12  EIMSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLH 71

Query: 62  DSVQCISDRLLRQLRRRDTVVVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCQ 121
           DSVQ IS++L+RQ+RRRDTV+VLCS GL+LVQLIYGCQRAGLVSVPISPPDP  EN+NC 
Sbjct: 72  DSVQFISEQLIRQVRRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFENKNCH 131

Query: 122 HLARAFSQTKPRAAIAHQSYINTIFRYLSSSPSDEKLALLLQSVKWISMDSLK-----QP 181
           HLARA SQTKPRAAIAHQ+YI ++FRYLS SP+D KLALLL+ V+WISM++L+     + 
Sbjct: 132 HLARALSQTKPRAAIAHQAYIASVFRYLSLSPTDGKLALLLRGVQWISMETLRTCTAAEA 191

Query: 182 HKEVEVNQNKHQPILYHS-SSYYGCKPEETYLIQYTSGATAIPKPVVITAGAAAHNVRAA 241
            + V V  +K QP  YHS SSYYGCKPEE YLIQYTSGAT IPKPVV+TAGAAAHNVR A
Sbjct: 192 DQVVSVISSKDQPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRVA 251

Query: 242 RKAYDLNPNDVIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTRPTTWLHLITAFK 301
           RKAYDLNPNDVIVSWLPQYHDCGLMFLLLTV++GATCVLTSPISFVTRP TWLHLIT FK
Sbjct: 252 RKAYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFK 311

Query: 302 ATCTPVPSFTLPLVLKRVKEETSSSRGLDLGSLRNLILINEPIYRSAVEEFVDVFKAVGL 361
           ATCTPVPSFTLPLVLKRVKEET    GLDL SLRNLILINEP+YRS VEEFVDVFK VGL
Sbjct: 312 ATCTPVPSFTLPLVLKRVKEETPPCGGLDLCSLRNLILINEPVYRSGVEEFVDVFKTVGL 371

Query: 362 DPGCVSPSYGLAENCTFVSTAWCG--GGDRRWLFPAMASYRKLLPSARLRDGSCTEIEVV 421
           +PGCVSPSYGLAENCTFVSTAW G  GG RRW FPAM SYRKLLP A+L D S  EIEVV
Sbjct: 372 NPGCVSPSYGLAENCTFVSTAWSGGEGGRRRW-FPAMPSYRKLLPCAKLTDESSAEIEVV 431

Query: 422 VVNGETGEVVEDGVEGEIWVSSPSNATAFDESYRYLEHPSLSRETFNSKINNKSSPNFVR 481
           VVNGETGE+V+DGVEGEIW+SSPSNA+       YL HPSL+R+TF+ K++NKSS  FVR
Sbjct: 432 VVNGETGEIVDDGVEGEIWISSPSNASG------YLGHPSLTRDTFHCKLSNKSSHKFVR 491

Query: 482 TGDRGVIKGADRFLFVIGRCSDVIKFNNNQQEIHPHYIESIAYNNSSAYLRGGCIAAVKI 541
           TGDRGVIKG DRFLFVIGRCSDVIK NNN QEIHPHYIESIAYNN SAYLRGGC+AA+KI
Sbjct: 492 TGDRGVIKGTDRFLFVIGRCSDVIKLNNN-QEIHPHYIESIAYNNFSAYLRGGCLAAIKI 551

Query: 542 SETIALVAEMQRDDKNDVELLKNICQEIRKALLIEEGIELGVVVLVKRGNVPKTTSGKVK 601
           S+TIA+VAEMQRDDKND E L+ IC+ IRKA LIEEGIELG+VVLVKRGNVPKTTSGKVK
Sbjct: 552 SDTIAVVAEMQRDDKNDAESLRKICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVK 611

Query: 602 RWAVKEKLAGGGLGVLMAVKFEKNCDVLMDLERKEEFRTRPLLLSLL 641
           RWA KEKLAGGG+ VLMAVKF   C V  DLE       RPLLLSLL
Sbjct: 612 RWAAKEKLAGGGMSVLMAVKFGTTCGVTKDLE-----GNRPLLLSLL 645

BLAST of Lsi08G013910 vs. NCBI nr
Match: KAG7029389.1 (fadD26, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1003.8 bits (2594), Expect = 6.6e-289
Identity = 516/645 (80.00%), Postives = 555/645 (86.05%), Query Frame = 0

Query: 4   MLYENFDPLFPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTADAVNEGSFLTYRQLHDS 63
           M YENFDP+FPDQPVVD YLPVWASLPAFRSKPAFIWSEDGTAD +NEGSFLTY QLHDS
Sbjct: 1   MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 60

Query: 64  VQCISDRLLRQLRRRDTVVVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCQHL 123
           VQ ISD L+RQ+RRRDTVV+LCS GL+LVQLIYGCQRAGLVSVPISPPDP  ENENC HL
Sbjct: 61  VQFISDELIRQVRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPRFENENCHHL 120

Query: 124 ARAFSQTKPRAAIAHQSYINTIFRYLSSSPSDEKLALLLQSVKWISMDSLK-----QPHK 183
           ARA SQTKP AAIAHQ+YI ++FRYLS SP D KLALLL+SV+WISM+SL+     +  +
Sbjct: 121 ARALSQTKPWAAIAHQAYIASVFRYLSLSPIDGKLALLLRSVQWISMESLRTCTAAEADQ 180

Query: 184 EVEVNQNKHQPILYHS-SSYYGCKPEETYLIQYTSGATAIPKPVVITAGAAAHNVRAARK 243
            V V   K QP  YHS SSYYGCKPEE YLIQYTSGAT IPKPVV+TAGAAAHNVR ARK
Sbjct: 181 VVSVISRKDQPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRVARK 240

Query: 244 AYDLNPNDVIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTRPTTWLHLITAFKAT 303
           AYDLNPNDVIVSWLPQYHDCGLMFLLLTV++GATCVLTSPISFVTRP TWLHLIT FKAT
Sbjct: 241 AYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKAT 300

Query: 304 CTPVPSFTLPLVLKRVKEETSSSRGLDLGSLRNLILINEPIYRSAVEEFVDVFKAVGLDP 363
           CTPVPSFTLPLVLKRVKEET    GLDL SLRNLILINEP+YRS VEEFVDVFKAVGL+P
Sbjct: 301 CTPVPSFTLPLVLKRVKEETPPCGGLDLCSLRNLILINEPVYRSGVEEFVDVFKAVGLNP 360

Query: 364 GCVSPSYGLAENCTFVSTAWCG--GGDRRWLFPAMASYRKLLPSARLRDGSCTEIEVVVV 423
           GCVSPSYGLAENCTFVSTAW G  GG RRW FPAM SYRKLLPSARL D S +EIEVVVV
Sbjct: 361 GCVSPSYGLAENCTFVSTAWSGGEGGRRRW-FPAMPSYRKLLPSARLMDESSSEIEVVVV 420

Query: 424 NGETGEVVEDGVEGEIWVSSPSNATAFDESYRYLEHPSLSRETFNSKINNKSSPNFVRTG 483
           NGETGE+VEDGVEGEIW+SSPSNA+       YL HPSL+R+TF+ K++NKSS  FVRTG
Sbjct: 421 NGETGEIVEDGVEGEIWISSPSNASG------YLGHPSLTRDTFHCKLSNKSSHKFVRTG 480

Query: 484 DRGVIKGADRFLFVIGRCSDVIKFNNNQQEIHPHYIESIAYNNSSAYLRGGCIAAVKISE 543
           DRGVIKG DRFLFVIGRCSDVI   NN QEIHPHYIESIAYNN S+YLRGGC+AA+KIS+
Sbjct: 481 DRGVIKGTDRFLFVIGRCSDVITLTNN-QEIHPHYIESIAYNNCSSYLRGGCLAAIKISD 540

Query: 544 TIALVAEMQRDDKNDVELLKNICQEIRKALLIEEGIELGVVVLVKRGNVPKTTSGKVKRW 603
           TIA+VAEMQR DKND E L+ IC+ IRKA LIEEGIELG+VVLVKRGNVPKTTSGKVKRW
Sbjct: 541 TIAVVAEMQRQDKNDAESLRKICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRW 600

Query: 604 AVKEKLAGGGLGVLMAVKFEKNCDVLMDLERKEEFRTRPLLLSLL 641
           A KEKLAGGG+ VLMAVKF K C +  DLE       RPLLLS L
Sbjct: 601 AAKEKLAGGGMSVLMAVKFGKTCGITKDLE-----TNRPLLLSFL 632

BLAST of Lsi08G013910 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 65.9 bits (159), Expect = 1.4e-10
Identity = 96/413 (23.24%), Postives = 170/413 (41.16%), Query Frame = 0

Query: 202 PEETYLIQYTSGATAIPKPVVIT----AGAAAHNVRAARKAYDLNPNDVIVSWLPQYHDC 261
           PE+T  + Y+SG T +PK V+IT      + A  V       +   NDVI+ +LP +H  
Sbjct: 209 PEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVILCFLPMFHIY 268

Query: 262 GLMFLLLTVI-TGATCVLTSPISFVTRPTTWLHLITAFKATCTPVPSFTLPLVLKRVKEE 321
            L  L+L+ + TGA  ++             + LI  +K T  PV     P+VL  +K  
Sbjct: 269 ALDALMLSAMRTGAALLIVPRFEL----NLVMELIQRYKVTVVPVAP---PVVLAFIK-- 328

Query: 322 TSSSRGLDLGSLRNLILINEPIYRSAVEEFVDVFKAVGLDPGCVSPSYGLAENCTFVSTA 381
           +  +   DL S+R ++L      +  +E+ V     +          YG+ E+ T   + 
Sbjct: 329 SPETERYDLSSVR-IMLSGAATLKKELEDAV----RLKFPNAIFGQGYGMTESGTVAKS- 388

Query: 382 WCGGGDRRWLFPAMASYRKLLPSARLRDGSCTEI----EVVVVNGETGEVVEDGVEGEIW 441
                          ++ K     + + G+C  +    E+ VV+ ETG  +     GEI 
Sbjct: 389 --------------LAFAK--NPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEIC 448

Query: 442 VSSPSNATAFDESYRYLEHPSLSRETFNSKINNKSSPNFVRTGDRGVIKGADRFLFVIGR 501
           V              YL  P  +  T +          ++ TGD G +   D  +F++ R
Sbjct: 449 VRGHQLMKG------YLNDPEATARTID-------KDGWLHTGDIGFVDDDDE-IFIVDR 508

Query: 502 CSDVIKFNNNQQEIHPHYIESIAYNNSSAYLRGGCIAAVKISET----IALVAEMQRDDK 561
             ++IKF   Q  + P  +E++  ++ S  +    + A+K        +A VA  Q    
Sbjct: 509 LKELIKFKGYQ--VAPAELEALLISHPS--IDDAAVVAMKDEVADEVPVAFVARSQGSQL 564

Query: 562 NDVELLKNICQEIRKALLIEEGIELGVVVLVKRGNVPKTTSGKVKRWAVKEKL 602
            +     ++   + K ++  + I++   + V    +PK  SGK+ R  ++ KL
Sbjct: 569 TE----DDVKSYVNKQVVHYKRIKMVFFIEV----IPKAVSGKILRKDLRAKL 564

BLAST of Lsi08G013910 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 59.3 bits (142), Expect = 1.3e-08
Identity = 99/423 (23.40%), Postives = 172/423 (40.66%), Query Frame = 0

Query: 202 PEETYLIQYTSGATAIPKPVVITAGAAAHNVRAARKAYDLNPN------DVIVSWLPQYH 261
           P++   + Y+SG T +PK V++T      +V  A++    NPN      DVI+  LP +H
Sbjct: 201 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSV--AQQVDGENPNLYFHSDDVILCVLPMFH 260

Query: 262 DCGLMFLLLTVITGATCVLTSPISFVTRPTTWLHLITAFKATCTPVPSFTLPLVLKRVKE 321
              L  ++L  +     +L  P   +      L LI   K T  P+     P+VL   K 
Sbjct: 261 IYALNSIMLCGLRVGAAILIMPKFEI---NLLLELIQRCKVTVAPM---VPPIVLAIAK- 320

Query: 322 ETSSSRGLDLGSLRNLILINEPIYRSAVEEFVDVFKAVGLDPGCVSPSYGLAENCTFVST 381
            +S +   DL S+R +     P+ +   +     F    L  G     YG+ E    ++ 
Sbjct: 321 -SSETEKYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQG-----YGMTEAGPVLAM 380

Query: 382 AWCGGGDRRWLFPAMASYRKLLPSARLRDGSCTEI----EVVVVNGETGEVVEDGVEGEI 441
           +    G  +  FP             ++ G+C  +    E+ +V+ +TG+ +     GEI
Sbjct: 381 SL---GFAKEPFP-------------VKSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEI 440

Query: 442 WVSSPSNATAFDESYRYLEHPSLSRETFNSKINNKSSPNFVRTGDRGVIKGADRFLFVIG 501
            +              YL +P+ + ET +          ++ TGD G+I   D  LF++ 
Sbjct: 441 CIRGHQIMKG------YLNNPAATAETID-------KDGWLHTGDIGLIDDDDE-LFIVD 500

Query: 502 RCSDVIKFNNNQQEIHPHYIESIAYNNSSAYLRGGCIAAVKISETIALVAEMQRDDKNDV 561
           R  ++IK+   Q  + P  +E++   +                 T   V  M+ +   +V
Sbjct: 501 RLKELIKYKGFQ--VAPAELEALLIGHPDI--------------TDVAVVAMKEEAAGEV 560

Query: 562 ELLKNICQEIRKALLIEEGIELGV---VVLVKRGN-------VPKTTSGKVKRWAVKEKL 605
            +      + + + L E+ ++  V   VV  KR N       +PK  SGK+ R  ++ KL
Sbjct: 561 PVA--FVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKL 560

BLAST of Lsi08G013910 vs. TAIR 10
Match: AT1G51680.2 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 55.5 bits (132), Expect = 1.9e-07
Identity = 77/323 (23.84%), Postives = 135/323 (41.80%), Query Frame = 0

Query: 202 PEETYLIQYTSGATAIPKPVVITAGAAAHNVRAARKAYDLNPN------DVIVSWLPQYH 261
           P++   + Y+SG T +PK V++T      +V  A++    NPN      DVI+  LP +H
Sbjct: 201 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSV--AQQVDGENPNLYFHSDDVILCVLPMFH 260

Query: 262 DCGLMFLLLTVITGATCVLTSPISFVTRPTTWLHLITAFKATCTPVPSFTLPLVLKRVKE 321
              L  ++L  +     +L  P   +      L LI   K T  P+     P+VL   K 
Sbjct: 261 IYALNSIMLCGLRVGAAILIMPKFEI---NLLLELIQRCKVTVAPM---VPPIVLAIAK- 320

Query: 322 ETSSSRGLDLGSLRNLILINEPIYRSAVEEFVDVFKAVGLDPGCVSPSYGLAENCTFVST 381
            +S +   DL S+R +     P+ +   +     F    L  G     YG+ E    ++ 
Sbjct: 321 -SSETEKYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQG-----YGMTEAGPVLAM 380

Query: 382 AWCGGGDRRWLFPAMASYRKLLPSARLRDGSCTEI----EVVVVNGETGEVVEDGVEGEI 441
           +    G  +  FP             ++ G+C  +    E+ +V+ +TG+ +     GEI
Sbjct: 381 SL---GFAKEPFP-------------VKSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEI 440

Query: 442 WVSSPSNATAFDESYRYLEHPSLSRETFNSKINNKSSPNFVRTGDRGVIKGADRFLFVIG 501
            +              YL +P+ + ET +          ++ TGD G+I   D  LF++ 
Sbjct: 441 CIRGHQIMKG------YLNNPAATAETID-------KDGWLHTGDIGLIDDDDE-LFIVD 476

Query: 502 RCSDVIKFNNNQQEIHPHYIESI 515
           R  ++IK+   Q  + P  +E++
Sbjct: 501 RLKELIKYKGFQ--VAPAELEAL 476

BLAST of Lsi08G013910 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 54.7 bits (130), Expect = 3.2e-07
Identity = 83/381 (21.78%), Postives = 160/381 (41.99%), Query Frame = 0

Query: 202 PEETYLIQYTSGATAIPKPVVITAGAAAHNVRAARKAYDLNPN------DVIVSWLPQYH 261
           P++   + Y+SG T +PK V++T      +V  A++    NPN      DVI+  LP +H
Sbjct: 201 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSV--AQQVDGENPNLYFHSDDVILCVLPMFH 260

Query: 262 DCGLMFLLLTVITGATCVLTSPISFVTRPTTWLHLITAFKATCTPVPSFTLPLVLKRVKE 321
              L  ++L  +     +L  P   +      L LI   K T  P+     P+VL   K 
Sbjct: 261 IYALNSIMLCGLRVGAAILIMPKFEI---NLLLELIQRCKVTVAPM---VPPIVLAIAK- 320

Query: 322 ETSSSRGLDLGSLRNLILINEPIYRSAVEEFVDVFKAVGLDPGCVSPSYGLAENCTFVST 381
            +S +   DL S+R +     P+ +   +     F    L  G     YG+ E    ++ 
Sbjct: 321 -SSETEKYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQG-----YGMTEAGPVLAM 380

Query: 382 AWCGGGDRRWLFPAMASYRKLLPSARLRDGSCTEI----EVVVVNGETGEVVEDGVEGEI 441
           +    G  +  FP             ++ G+C  +    E+ +V+ +TG+ +     GEI
Sbjct: 381 SL---GFAKEPFP-------------VKSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEI 440

Query: 442 WVSSPSNATAFDESYRYLEHPSLSRETFNSKINNKSSPNFVRTGDRGVIKGADRFLFVIG 501
            +              YL +P+ + ET +          ++ TGD G+I   D  LF++ 
Sbjct: 441 CIRGHQIMKG------YLNNPAATAETID-------KDGWLHTGDIGLIDDDDE-LFIVD 500

Query: 502 RCSDVIKFNNNQQEIHPHYIESIAYNNSSAYLRGGCIAAVKISET----IALVAEMQRDD 561
           R  ++IK+   Q  + P  +E++   +    +    + A+K        +A V + +  +
Sbjct: 501 RLKELIKYKGFQ--VAPAELEALLIGHPD--ITDVAVVAMKEEAAGEVPVAFVVKSKDSE 532

Query: 562 KNDVELLKNICQEIRKALLIE 569
            ++ ++ + + ++++  +L E
Sbjct: 561 LSEDDVKQFVSKQVKSCVLQE 532

BLAST of Lsi08G013910 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 52.4 bits (124), Expect = 1.6e-06
Identity = 96/425 (22.59%), Postives = 166/425 (39.06%), Query Frame = 0

Query: 202 PEETYLIQYTSGATAIPKPVVIT----AGAAAHNVRAARKAYDLNPNDVIVSWLPQYHDC 261
           PE+   + ++SG T +PK V++T      + A  V         N +DVI+  LP +H  
Sbjct: 194 PEDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIY 253

Query: 262 GLMFLLLTVITGATCVLTSPISFVTRPTTWLHLITAFKATCTPVPSFTLPLVLKRVKEET 321
            L  ++L  +     +L  P   +   T  L  I   K T   V     P+VL   K  +
Sbjct: 254 ALNSIMLCSLRVGATILIMPKFEI---TLLLEQIQRCKVT---VAMVVPPIVLAIAK--S 313

Query: 322 SSSRGLDLGSLRNLILINEPIYRSAVEEFVDVFKAVGLDPGCVSPSYGLAENCTFVSTAW 381
             +   DL S+R +     P+ +   +     F    L  G     YG+ E    ++ + 
Sbjct: 314 PETEKYDLSSVRMVKSGAAPLGKELEDAISAKFPNAKLGQG-----YGMTEAGPVLAMSL 373

Query: 382 CGGGDRRWLFPAMASYRKLLPSARLRDGSCTEI----EVVVVNGETGEVVEDGVEGEIWV 441
              G  +  FP             ++ G+C  +    E+ +++ +TG+ +     GEI +
Sbjct: 374 ---GFAKEPFP-------------VKSGACGTVVRNAEMKILDPDTGDSLPRNKPGEICI 433

Query: 442 SSPSNATAFDESYRYLEHPSLSRETFNSKINNKSSPNFVRTGDRGVIKGADRFLFVIGRC 501
                         YL  P  +  T +          ++ TGD G I   D  LF++ R 
Sbjct: 434 RGNQIMKG------YLNDPLATASTID-------KDGWLHTGDVGFIDDDDE-LFIVDRL 493

Query: 502 SDVIKFNNNQQEIHPHYIESIAYNNSSAYLRGGCIAAVKISETIALVAEMQRDDKNDVEL 561
            ++IK+   Q  + P  +ES+   +                  +A+VA M+ +D  +V +
Sbjct: 494 KELIKYKGFQ--VAPAELESLLIGHPEI-------------NDVAVVA-MKEEDAGEVPV 553

Query: 562 L-------KNICQEIRKALLIEEGIELGVVVLVKRGN-------VPKTTSGKVKRWAVKE 605
                    NI ++  K  + ++      VV  KR N       +PK  SGK+ R  ++ 
Sbjct: 554 AFVVRSKDSNISEDEIKQFVSKQ------VVFYKRINKVFFTDSIPKAPSGKILRKDLRA 553