PI0014254 (gene) Melon (PI 482460) v1

BLAST of PI0014254 vs. ExPASy Swiss-Prot
Match: Q9FI25 (Berberine bridge enzyme-like 27 OS=Arabidopsis thaliana OX=3702 GN=At5g44410 PE=2 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 1.1e-129
Identity = 241/508 (47.44%), Postives = 329/508 (64.76%), Query Frame = 0

Query: 2   EDFVNCIYFHSSNNNDSNSTIIHTPSSSSYSYVLNFSIRNLRF----------------- 61
           E+F+ C+   S   N+ +S IIHT    SY  +LN SI+N RF                 
Sbjct: 35  ENFLKCL---SHRINEDDSRIIHTSKDPSYFSILNSSIQNPRFFVLETPKPVSIITPVQA 94

Query: 62  ----SEPKLPNHHGLQIRTRSGGHDFEGRSYVA-NVPFVLIDLINLNSITIDIEDETAWV 121
               S  K    HG+ IRTRSGGHD+EG SY+A + PFV+IDL NL SIT+D+++ T WV
Sbjct: 95  TDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITLDVDNRTGWV 154

Query: 122 QSGATVGELYYRIGEKSRTLGFPAGFTATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYV 181
           QSGAT+GELYY IG+ S++L FPAG   T+G+GG   GGG G ++RKYGL ADNV+DA++
Sbjct: 155 QSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLSADNVIDAHI 214

Query: 182 VDGNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQNAA 241
           VD NG  ++R  MGED FWAIRGGGG SF +VL+WK+RL+ VPS+VT F + KT ++ A 
Sbjct: 215 VDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKVVKTSEKEAV 274

Query: 242 NLIYRWQYIAPWVNQDLFISAWVTASNSSDGSGRIMQASFFSLFLGNATELLSLMEKTFP 301
           ++I +WQYIA  V  DLFI A +            + ASF  L+LG  ++LL+LM+  FP
Sbjct: 275 SIINKWQYIADKVPNDLFIRAMLQKETE-------VYASFPGLYLGPVSDLLALMKDKFP 334

Query: 302 ELGLKKEDCLEMSWVESMAFSASGFVSAKSLELLLDRTPLNNGRYKTKSDYATQPISETA 361
           ELGL+  +C EMSW+ES+ +    F+  +S+E+L  R   +   +K K D+  +PI +TA
Sbjct: 335 ELGLEIGNCREMSWIESVLW----FIKGESMEILAKRKRTSRS-FKGKDDFIEEPIPKTA 394

Query: 362 LEGMWERFKDEELETVQLILIPFGGKMNEISESETPFPHRAGYPIHISYYLTW-----QR 421
           ++ +W RF+  E    ++IL PFGGKM+EI+++E PFPHR G    I Y   W     + 
Sbjct: 395 IQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQYLAYWSEEEDKN 454

Query: 422 PNADSRHLEWARELHNYMTPFVSKSPRAAYVNYKDLDMGTNNDDRVPTRYEEASVWGHRY 481
                ++L W   ++ +MTP+VSKSPR AYVN++D+D+G      + T+YEEA VWG +Y
Sbjct: 455 KTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGLNMKTKYEEAKVWGVKY 514

Query: 482 FGNNFERLMEVKTKVDPFNFFRHEQSIP 483
           F NNF+RL+ VKT VDP +FF  EQSIP
Sbjct: 515 FKNNFDRLVRVKTNVDPMDFFCDEQSIP 527

BLAST of PI0014254 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 1.8e-127
Identity = 237/503 (47.12%), Postives = 330/503 (65.61%), Query Frame = 0

Query: 4   FVNCIYFHSSNNNDSN--STIIHTPSSSSYSYVLNFSIRNLRFSEPKLP----------- 63
           F+ C+   S   NDSN  S +IHTP+ +S+S VL  SI+N RFS P +P           
Sbjct: 37  FLQCL---SLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLILTPVQP 96

Query: 64  ----------NHHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDIEDETAWVQ 123
                        G+ IRTRSGGHD+EG SYV + PFV++DL NL SIT+D+++ + WVQ
Sbjct: 97  SDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDNRSVWVQ 156

Query: 124 SGATVGELYYRIGEKSRTLGFPAGFTATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYVV 183
           +GAT+GELYY IG+K+RTL FPAG   T+G+GG  SGGG G ++RK+GL AD+V+DA VV
Sbjct: 157 TGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHVIDARVV 216

Query: 184 DGNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQNAAN 243
           D  GR++ R  MGED FWAIRGGGG SF +VL+WK+ L+ VPS VT F + K  +Q+A  
Sbjct: 217 DARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFSEQSALK 276

Query: 244 LIYRWQYIAPWVNQDLFISAWVTASNSSDGSGRIMQASFFSLFLGNATELLSLMEKTFPE 303
           +I+RWQ++A  V+ DLFI   +    +      +++ASF  L+LG+   LL ++ K FPE
Sbjct: 277 IIHRWQFVADKVSDDLFIRVMLQRYKN------MVRASFPGLYLGSVKNLLKMVNKEFPE 336

Query: 304 LGLKKEDCLEMSWVESMAFSASGFVSAKSLELLLDRTPLNNGRYKTKSDYATQPISETAL 363
           LGL+++DC EMSW+ES+ + A   +  + + +L  RT  +   +K KSD+  +P+ +TA+
Sbjct: 337 LGLEEDDCTEMSWIESVIWFAE--LGEEPINVLTKRTRASLA-FKAKSDFVQEPMPKTAI 396

Query: 364 EGMWERFKDEELETVQLILIPFGGKMNEISESETPFPHRAGYPIHISYYLTWQRPNADSR 423
             +W R ++ E E  QLI  PFGGKM+EI++ ETPFPHR G    I Y   W R +   +
Sbjct: 397 SKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNYW-RGDVKEK 456

Query: 424 HLEWARELHNYMTPFVSKSPRAAYVNYKDLDMGTNNDDRVPTRYEEASVWGHRYFGNNFE 483
           ++ W   +++ M+ FV+KSPR AY+N +DLD+G     +  ++YEE   WG +YF NNFE
Sbjct: 457 YMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVGVK-RSKYEEGKSWGVKYFKNNFE 516

BLAST of PI0014254 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 3.9e-127
Identity = 233/514 (45.33%), Postives = 326/514 (63.42%), Query Frame = 0

Query: 2   EDFVNCIYFHSSNNNDSNSTIIHTPSSSSYSYVLNFSIRNLRFSEPKLP----------N 61
           E+F+ C   H   NN +N  +++T     Y  +LN +I+NLRF     P          N
Sbjct: 32  ENFLKCFSKHIP-NNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTPSNN 91

Query: 62  HH-----------GLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDIEDETAWVQ 121
            H           GLQIRTRSGGHD EG SY++ VPFV++DL N++SI ID+  +TAWV+
Sbjct: 92  SHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAWVE 151

Query: 122 SGATVGELYYRIGEKSRTLGFPAGFTATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYVV 181
           +GAT+GE+YY I EK+  L FP G+  T+G+GG  SGGG G ++R YGL ADN++DA++V
Sbjct: 152 AGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLV 211

Query: 182 DGNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWD-QNAA 241
           + +G+V++R SMGEDLFWAIRGGGG +FGI+ AWK++LV VPS  T F++ K  +     
Sbjct: 212 NVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIHGLV 271

Query: 242 NLIYRWQYIAPWVNQDLFISAWVTASNSSDGSGR---IMQASFFSLFLGNATELLSLMEK 301
            L  +WQ IA   ++DL +       N +D  G+    +   F S+F G    L+ LM K
Sbjct: 272 KLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLMNK 331

Query: 302 TFPELGLKKEDCLEMSWVESMAFSASGFVSAKSL----ELLLDRTPLNNGRYKTKSDYAT 361
           +FPELG+KK DC E SW+++  F  SG V+  +     E+LLDR+      +  K DY  
Sbjct: 332 SFPELGIKKTDCKEFSWIDTTIF-YSGVVNFNTANFKKEILLDRSAGKKTAFSIKLDYVK 391

Query: 362 QPISETALEGMWERFKDEELETVQLILIPFGGKMNEISESETPFPHRAGYPIHISYYLTW 421
           +PI ETA+  + E+  +E++     +L P+GG M EISES  PFPHRAG    + Y  +W
Sbjct: 392 KPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTASW 451

Query: 422 QRPNADSRHLEWARELHNYMTPFVSKSPRAAYVNYKDLDMGTNNDDRVPTRYEEASVWGH 481
           ++   + +H+ W R ++N+ TP+VS++PR AY+NY+DLD+G  N    P  Y +A +WG 
Sbjct: 452 EKQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTN-HASPNNYTQARIWGE 511

Query: 482 RYFGNNFERLMEVKTKVDPFNFFRHEQSIPPAPP 487
           +YFG NF RL++VKTKVDP NFFR+EQSIPP PP
Sbjct: 512 KYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPLPP 542

BLAST of PI0014254 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 1.5e-126
Identity = 227/515 (44.08%), Postives = 330/515 (64.08%), Query Frame = 0

Query: 2   EDFVNCIYFHSSNNNDSNSTIIHTPSSSSYSYVLNFSIRNLRFSEPKLP----------- 61
           E+F+ C+  +    N +N+ +++T     Y  +LN +++NLRF+    P           
Sbjct: 32  ENFLKCLSQYIP-TNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITTPLNV 91

Query: 62  ----------NHHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDIEDETAWVQ 121
                        GLQIRTRSGGHD EG SY++ VPFV++DL N++S+ ID+  +TAWV+
Sbjct: 92  SHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVE 151

Query: 122 SGATVGELYYRIGEKSRTLGFPAGFTATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYVV 181
           SGAT+GE+YY I E +  L FPAG+  T+G GG  SGGG G ++R YGL ADN++DA++V
Sbjct: 152 SGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNIIDAHLV 211

Query: 182 DGNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWD-QNAA 241
           + +G+V++R SMGEDLFWAIRGGGG +FGI+ AWK+RLV VPS+ T F++ K  +     
Sbjct: 212 NVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELV 271

Query: 242 NLIYRWQYIAPWVNQDLFISAWVTASNSSDGSGR---IMQASFFSLFLGNATELLSLMEK 301
            L+ +WQ IA    ++L +       N +D  G+    + + F S+F G    L+ LM K
Sbjct: 272 KLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNK 331

Query: 302 TFPELGLKKEDCLEMSWVESMAFSASGFVSAKSL----ELLLDRTPLNNGRYKTKSDYAT 361
           +FPELG+KK DC ++SW++++ F  SG V+  +     E+LLDR+      +  K DY  
Sbjct: 332 SFPELGIKKTDCKQLSWIDTIIF-YSGVVNYNTTNFKKEILLDRSGGRKAAFSIKLDYVK 391

Query: 362 QPISETALEGMWERFKDEELETVQLILIPFGGKMNEISESETPFPHRAGYPIHISYYLTW 421
           +PI ETA+  + E+  +E++     +  P+GG M+EISES  PFPHRAG    I Y  +W
Sbjct: 392 KPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIWYIASW 451

Query: 422 QRPNADSRHLEWARELHNYMTPFVSKSPRAAYVNYKDLDMGTNNDDRVPTRYEEASVWGH 481
           ++   + +H+ W R ++N+ TP+VS++PR AY+NY+DLD+G  N +  P  Y +A +WG 
Sbjct: 452 EKQEDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFES-PNNYTQARIWGE 511

Query: 482 RYFGNNFERLMEVKTKVDPFNFFRHEQSIPPAPPR 488
           +YFG NF RL++VKTKVDP NFFR+EQSIPP P R
Sbjct: 512 KYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPLPLR 543

BLAST of PI0014254 vs. ExPASy Swiss-Prot
Match: Q9FI21 (Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=1 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 2.6e-126
Identity = 237/510 (46.47%), Postives = 327/510 (64.12%), Query Frame = 0

Query: 2   EDFVNCIYFHSSNNNDSNSTIIHTPSSSSYSYVLNFSIRNLRFSEPKLPNH--------- 61
           EDF+ C+ +   N+N     +IHT   SS+  +L+ SI+N RFS  + P           
Sbjct: 29  EDFLKCLSYR-MNDNTVEPKVIHTSKDSSFFSILDSSIQNPRFSVSETPKPVSIITPVKA 88

Query: 62  ------------HGLQIRTRSGGHDFEGRSYVA-NVPFVLIDLINLNSITIDIEDETAWV 121
                       HG+ +RTRS GH +EG SY+A N PF +IDL NL SI++D+++ T WV
Sbjct: 89  SDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPFAVIDLRNLRSISLDVDNRTGWV 148

Query: 122 QSGATVGELYYRIGEKSRTLGFPAGFTATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYV 181
           Q+GAT GELYY IG+ +++L FPAG   T+G+GG  SGGG G ++RKYGL ADN++DA V
Sbjct: 149 QTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGYGTLLRKYGLAADNIIDALV 208

Query: 182 VDGNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQNAA 241
           VD +GR+++R +MGED FWAIRGGGG SFG++L+WK++LV VPS +T F + KT  + A 
Sbjct: 209 VDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVDVPSTITVFKVQKTSKKEAV 268

Query: 242 NLIYRWQYIAPWVNQDLFISAWVTASNSSDGSGRIMQASFFSLFLGNATELLSLMEKTFP 301
            +I +WQY A  V  DLFI   +  SN +      + A F  L++G    LL+LME+ FP
Sbjct: 269 RIIKKWQYAADKVPDDLFIRTTLERSNKN-----AVHALFTGLYIGPVNNLLALMEEKFP 328

Query: 302 ELGLKKEDCLEMSWVESMAFSASGFVSAKSLELLL--DRTPLNNGRYKTKSDYATQPISE 361
           ELGL+KE C EMSW+ES+ + A  F   +SL +L   +RT L+   +K K D+  +PI E
Sbjct: 329 ELGLEKEGCEEMSWIESVLWFAD-FPKGESLGVLTNRERTSLS---FKGKDDFVQEPIPE 388

Query: 362 TALEGMWERFKDEELETVQLILIPFGGKMNEISESETPFPHRAGYPIHISYYLTW----- 421
            A++ +W R +  E    ++IL PFGGKM+E++E ETPFPHR G    I Y   W     
Sbjct: 389 AAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPFPHRGGNLYEIQYVAYWREEED 448

Query: 422 QRPNADSRHLEWARELHNYMTPFVSKSPRAAYVNYKDLDMGTNNDDRVPTRYEEASVWGH 481
           +      ++L+W   ++ +MTP+VSKSPR AYVN+KD+D+G     +  T+YEE   WG 
Sbjct: 449 KNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMDLGMYLGKK-KTKYEEGKSWGV 508

Query: 482 RYFGNNFERLMEVKTKVDPFNFFRHEQSIP 483
           +YF NNFERL+ VKT+VDP +FF  EQSIP
Sbjct: 509 KYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of PI0014254 vs. ExPASy TrEMBL
Match: A0A5D3D0K7 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2273G00130 PE=3 SV=1)

HSP 1 Score: 926.8 bits (2394), Expect = 3.8e-266
Identity = 458/511 (89.63%), Postives = 477/511 (93.35%), Query Frame = 0

Query: 1   MEDFVNCIYFHSSNNNDSNSTIIHTPSSSSYSYVLNFSIRNLRFSEPKLP---------- 60
           MEDFV+CIY HSSNNNDSNST+IHTPSSSSYSYVLNFSIRNLRFSEP+ P          
Sbjct: 1   MEDFVDCIYVHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPVAIITPSH 60

Query: 61  -----------NHHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDIEDETAWV 120
                        HGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITID+EDETAWV
Sbjct: 61  ASQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDVEDETAWV 120

Query: 121 QSGATVGELYYRIGEKSRTLGFPAGFTATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYV 180
           QSGATVGELYY+IGEKSRTLGFPAGF ATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYV
Sbjct: 121 QSGATVGELYYKIGEKSRTLGFPAGFKATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYV 180

Query: 181 VDGNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQNAA 240
           VD NGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQNAA
Sbjct: 181 VDRNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQNAA 240

Query: 241 NLIYRWQYIAPWVNQDLFISAWVTASNSS-DGSGRIMQASFFSLFLGNATELLSLMEKTF 300
           NLIYRWQYIAPWV+QDLFISAWVTASNSS D SGRIM+ASFFSLFLGNATELLSLMEKTF
Sbjct: 241 NLIYRWQYIAPWVDQDLFISAWVTASNSSKDESGRIMEASFFSLFLGNATELLSLMEKTF 300

Query: 301 PELGLKKEDCLEMSWVESMAFSASGFVSAKSLELLLDRTPLNNGRYKTKSDYATQPISET 360
           PELGLKK+DCLEMSWVESMAFSASGFV AKSLELLLDRTP++NGRYKTKSDYAT+PIS+T
Sbjct: 301 PELGLKKKDCLEMSWVESMAFSASGFVYAKSLELLLDRTPVDNGRYKTKSDYATEPISKT 360

Query: 361 ALEGMWERFKDEELETVQLILIPFGGKMNEISESETPFPHRAGYPIHISYYLTWQRPNAD 420
           AL+GMWERFKDEELETVQLILIPFGGKMNEISESETP PHRAGYPIHI YYLTWQ+PNAD
Sbjct: 361 ALKGMWERFKDEELETVQLILIPFGGKMNEISESETPSPHRAGYPIHIGYYLTWQQPNAD 420

Query: 421 SRHLEWARELHNYMTPFVSKSPRAAYVNYKDLDMGTNNDDRVPTRYEEASVWGHRYFGNN 480
           SR+LEWARELH+YMTPFVSKSPRAAYVNY+DLDMGTNNDD VPTRY+EAS+WGHRYFGNN
Sbjct: 421 SRNLEWARELHDYMTPFVSKSPRAAYVNYRDLDMGTNNDDGVPTRYKEASLWGHRYFGNN 480

Query: 481 FERLMEVKTKVDPFNFFRHEQSIPPAPPRVG 490
           FERLMEVK KVDPFNFFRHEQSIPPAPPRVG
Sbjct: 481 FERLMEVKMKVDPFNFFRHEQSIPPAPPRVG 511

BLAST of PI0014254 vs. ExPASy TrEMBL
Match: A0A1S3CIZ3 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103501494 PE=3 SV=1)

HSP 1 Score: 926.8 bits (2394), Expect = 3.8e-266
Identity = 458/511 (89.63%), Postives = 477/511 (93.35%), Query Frame = 0

Query: 1   MEDFVNCIYFHSSNNNDSNSTIIHTPSSSSYSYVLNFSIRNLRFSEPKLP---------- 60
           MEDFV+CIY HSSNNNDSNST+IHTPSSSSYSYVLNFSIRNLRFSEP+ P          
Sbjct: 1   MEDFVDCIYVHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPVAIITPSH 60

Query: 61  -----------NHHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDIEDETAWV 120
                        HGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITID+EDETAWV
Sbjct: 61  ASQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDVEDETAWV 120

Query: 121 QSGATVGELYYRIGEKSRTLGFPAGFTATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYV 180
           QSGATVGELYY+IGEKSRTLGFPAGF ATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYV
Sbjct: 121 QSGATVGELYYKIGEKSRTLGFPAGFKATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYV 180

Query: 181 VDGNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQNAA 240
           VD NGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQNAA
Sbjct: 181 VDRNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQNAA 240

Query: 241 NLIYRWQYIAPWVNQDLFISAWVTASNSS-DGSGRIMQASFFSLFLGNATELLSLMEKTF 300
           NLIYRWQYIAPWV+QDLFISAWVTASNSS D SGRIM+ASFFSLFLGNATELLSLMEKTF
Sbjct: 241 NLIYRWQYIAPWVDQDLFISAWVTASNSSKDESGRIMEASFFSLFLGNATELLSLMEKTF 300

Query: 301 PELGLKKEDCLEMSWVESMAFSASGFVSAKSLELLLDRTPLNNGRYKTKSDYATQPISET 360
           PELGLKK+DCLEMSWVESMAFSASGFV AKSLELLLDRTP++NGRYKTKSDYAT+PIS+T
Sbjct: 301 PELGLKKKDCLEMSWVESMAFSASGFVYAKSLELLLDRTPVDNGRYKTKSDYATEPISKT 360

Query: 361 ALEGMWERFKDEELETVQLILIPFGGKMNEISESETPFPHRAGYPIHISYYLTWQRPNAD 420
           AL+GMWERFKDEELETVQLILIPFGGKMNEISESETP PHRAGYPIHI YYLTWQ+PNAD
Sbjct: 361 ALKGMWERFKDEELETVQLILIPFGGKMNEISESETPSPHRAGYPIHIGYYLTWQQPNAD 420

Query: 421 SRHLEWARELHNYMTPFVSKSPRAAYVNYKDLDMGTNNDDRVPTRYEEASVWGHRYFGNN 480
           SR+LEWARELH+YMTPFVSKSPRAAYVNY+DLDMGTNNDD VPTRY+EAS+WGHRYFGNN
Sbjct: 421 SRNLEWARELHDYMTPFVSKSPRAAYVNYRDLDMGTNNDDGVPTRYKEASLWGHRYFGNN 480

Query: 481 FERLMEVKTKVDPFNFFRHEQSIPPAPPRVG 490
           FERLMEVK KVDPFNFFRHEQSIPPAPPRVG
Sbjct: 481 FERLMEVKMKVDPFNFFRHEQSIPPAPPRVG 511

BLAST of PI0014254 vs. ExPASy TrEMBL
Match: A0A6J1HP64 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111464765 PE=3 SV=1)

HSP 1 Score: 786.6 bits (2030), Expect = 6.1e-224
Identity = 384/509 (75.44%), Postives = 442/509 (86.84%), Query Frame = 0

Query: 1   MEDFVNCIYFHSSNNNDSNSTIIHTPSSSSYSYVLNFSIRNLRFSEPKLP---------- 60
           ++DF++CI  HSSN++ SNST+IHTPSSSSY +VLNFSIRNLRF+E   P          
Sbjct: 49  LQDFLHCISLHSSNHH-SNSTLIHTPSSSSYPHVLNFSIRNLRFAESHTPKPVAIVTPWQ 108

Query: 61  -----------NHHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDIEDETAWV 120
                        HGLQIRTRSGGHDFEGRSYV+NVPFVLIDLINLNSITID+++ETAW 
Sbjct: 109 PSEVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNVPFVLIDLINLNSITIDVDNETAWA 168

Query: 121 QSGATVGELYYRIGEKSRTLGFPAGFTATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYV 180
           QSGATVGELYY+IGEKSRTL FPAGFTA+IGLGGF SGGG+GM+VRKYGL  DNVVDAY+
Sbjct: 169 QSGATVGELYYKIGEKSRTLAFPAGFTASIGLGGFFSGGGLGMLVRKYGLAVDNVVDAYL 228

Query: 181 VDGNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQNAA 240
           VD NG++++R SMGEDLFWAIRGGGGGSFGIVLAWKL+LV+VP  VTSFA+HK+WD NAA
Sbjct: 229 VDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVRVPPTVTSFAVHKSWDLNAA 288

Query: 241 NLIYRWQYIAPWVNQDLFISAWVTASNSS--DGSGRIMQASFFSLFLGNATELLSLMEKT 300
            LI+RWQY+AP V++DLFI+AWVTASNSS  +G GR+M+ASFFSLFLG A EL+SLM+K 
Sbjct: 289 KLIHRWQYVAPKVDEDLFITAWVTASNSSEGEGGGRVMKASFFSLFLGKAAELVSLMKKA 348

Query: 301 FPELGLKKEDCLEMSWVESMAFSASGFVSAKSLELLLDRTPLNNGRYKTKSDYATQPISE 360
           FPEL LKKEDCLE+SWVE+MAFSAS +VS ++LELLLDRTPL  GRYK KSDYAT+PISE
Sbjct: 349 FPELELKKEDCLEISWVEAMAFSASEYVSPENLELLLDRTPLITGRYKMKSDYATKPISE 408

Query: 361 TALEGMWERFKDEELETVQLILIPFGGKMNEISESETPFPHRAGYPIHISYYLTWQRPNA 420
           TAL G+WERFK EELETVQLILIPFGGKMNEISE+ TP PHRA +PIHI YY+TW RP+A
Sbjct: 409 TALNGIWERFKIEELETVQLILIPFGGKMNEISETATPNPHRASHPIHIGYYVTWHRPDA 468

Query: 421 DSRHLEWARELHNYMTPFVSKSPRAAYVNYKDLDMGTNNDDRVPTRYEEASVWGHRYFGN 480
           DSRHLEW RELH+YMTPFVS+SPRAAY+NYKDLD+G NN+D +PT Y+EASVWG+RYFG 
Sbjct: 469 DSRHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGRNNEDGIPTSYDEASVWGYRYFGI 528

Query: 481 NFERLMEVKTKVDPFNFFRHEQSIPPAPP 487
           NFERL+EVKTKVDPFNFF+HE+SIPPA P
Sbjct: 529 NFERLVEVKTKVDPFNFFQHEESIPPAAP 556

BLAST of PI0014254 vs. ExPASy TrEMBL
Match: A0A6J1HYZ7 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111469396 PE=3 SV=1)

HSP 1 Score: 780.4 bits (2014), Expect = 4.4e-222
Identity = 385/510 (75.49%), Postives = 436/510 (85.49%), Query Frame = 0

Query: 1   MEDFVNCIYFHSSNNNDSNSTIIHTPSSSSYSYVLNFSIRNLRFSEPKLP---------- 60
           +EDF++CI  HSSN + SNST+IHTPSSSSY YVLNFSIRNLRF+E   P          
Sbjct: 44  LEDFLHCISLHSSNRH-SNSTLIHTPSSSSYPYVLNFSIRNLRFAEYHTPKPVAIVTPWQ 103

Query: 61  -----------NHHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDIEDETAWV 120
                        HGLQIRTRSGGHDFEGRSYV+N+PFVLIDLINLNSITID++DETAW 
Sbjct: 104 ASQVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNIPFVLIDLINLNSITIDVDDETAWA 163

Query: 121 QSGATVGELYYRIGEKSRTLGFPAGFTATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYV 180
           QSGATVGELYY+IGEKSRTL FPAG TA+IGLGGF SGGG+GM+VRKYGL  DNVVDAY+
Sbjct: 164 QSGATVGELYYKIGEKSRTLAFPAGITASIGLGGFFSGGGLGMLVRKYGLAVDNVVDAYL 223

Query: 181 VDGNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQNAA 240
           VD NG++++R SMGEDLFWAIRGGGGGSFGIVLAWKL+LV+VP  VTSFA+HK+WD NAA
Sbjct: 224 VDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVRVPPTVTSFAVHKSWDLNAA 283

Query: 241 NLIYRWQYIAPWVNQDLFISAWVTASNSSDGSGRIMQASFFSLFLGNATELLSLMEKTFP 300
            LI+ WQY+A  V++DLFI+A VTASNSS   GRIM+ASFFSLFLG A EL+SLMEK FP
Sbjct: 284 KLIHWWQYVALEVDEDLFITARVTASNSSKEGGRIMKASFFSLFLGKAAELVSLMEKAFP 343

Query: 301 ELGLKKEDCLEMSWVESMAFSASGFVSAKSLELLLDRTPLNNGRYKTKSDYATQPISETA 360
           EL LKKEDCLE+SWVE+MAFSAS +VS ++LELLLDRT L  GRYK KSDYAT+PISETA
Sbjct: 344 ELELKKEDCLEISWVEAMAFSASEYVSPENLELLLDRTSLITGRYKMKSDYATKPISETA 403

Query: 361 LEGMWERFKDEELETVQLILIPFGGKMNEISESETPFPHRAGYPIHISYYLTWQRPNADS 420
           L G+WERFK EELETVQLILIPFGGKMNEISE++TP PHRA +PIHI YY+TW RP+ADS
Sbjct: 404 LNGIWERFKIEELETVQLILIPFGGKMNEISETDTPNPHRASHPIHIGYYVTWHRPDADS 463

Query: 421 RHLEWARELHNYMTPFVSKSPRAAYVNYKDLDMGTNNDDRVPTRYEEASVWGHRYFGNNF 480
           RHLEW RELH+YMTPFVS+SPRAAY+NYKDLD+G NN+D +PT Y+EASVWG+RYFG NF
Sbjct: 464 RHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGKNNEDGIPTSYDEASVWGYRYFGINF 523

Query: 481 ERLMEVKTKVDPFNFFRHEQSIPPAPPRVG 490
           ERL+EVKTKVDPFNFFRHEQSIPPA P  G
Sbjct: 524 ERLVEVKTKVDPFNFFRHEQSIPPAAPMGG 552

BLAST of PI0014254 vs. ExPASy TrEMBL
Match: A0A6J1I038 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111469264 PE=3 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 1.4e-220
Identity = 381/508 (75.00%), Postives = 431/508 (84.84%), Query Frame = 0

Query: 1   MEDFVNCIYFHSSNNNDSNSTIIHTPSSSSYSYVLNFSIRNLRFSEPKLP---------- 60
           ++DF++CI  HSSN+N SNST+IHTPSSSSY YVLNFSIRNLRF+E   P          
Sbjct: 35  LQDFLHCISLHSSNHN-SNSTLIHTPSSSSYLYVLNFSIRNLRFAESDTPKPVAIVTPWQ 94

Query: 61  -----------NHHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDIEDETAWV 120
                        HGLQIRTRSGG DFEGRSYV+NVPFVLIDLINLNSITID EDETAW 
Sbjct: 95  ASQVQATVICCKTHGLQIRTRSGGQDFEGRSYVSNVPFVLIDLINLNSITIDTEDETAWA 154

Query: 121 QSGATVGELYYRIGEKSRTLGFPAGFTATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYV 180
           QSGATVGELYY+IG+KSRTL FPAG  A +GLGGF SGGG GM++RKYGL  DNVVDA +
Sbjct: 155 QSGATVGELYYKIGQKSRTLAFPAGLVANLGLGGFFSGGGFGMLLRKYGLAVDNVVDAQL 214

Query: 181 VDGNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQNAA 240
           VD NG++++R SMGEDLFWAIRGGGGGSFGIVL WKL+LV+VP  VTSFA+HK+WD NAA
Sbjct: 215 VDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLTWKLKLVRVPPTVTSFAVHKSWDLNAA 274

Query: 241 NLIYRWQYIAPWVNQDLFISAWVTASNSSDGSGRIMQASFFSLFLGNATELLSLMEKTFP 300
            LI++WQY+AP V++DLFI+AWVTASNSSDG GRIM+ASFF+LFLG A EL+SLM K FP
Sbjct: 275 KLIHQWQYVAPEVDEDLFITAWVTASNSSDGGGRIMKASFFALFLGEAAELVSLMAKAFP 334

Query: 301 ELGLKKEDCLEMSWVESMAFSASGFVSAKSLELLLDR-TPLNNGRYKTKSDYATQPISET 360
           EL L +EDCLEMSWVESMAFSAS FVSA++LELLLDR T L+NGRYK KSDYAT+PISET
Sbjct: 335 ELELTREDCLEMSWVESMAFSASVFVSAENLELLLDRTTQLSNGRYKIKSDYATEPISET 394

Query: 361 ALEGMWERFKDEELETVQLILIPFGGKMNEISESETPFPHRAGYPIHISYYLTWQRPNAD 420
           AL G+WERFK EELET+QLILIPFGGKMNEISE++TP PHRA +PIHI YY TW RP+AD
Sbjct: 395 ALNGIWERFKIEELETLQLILIPFGGKMNEISETDTPNPHRARHPIHIGYYATWHRPDAD 454

Query: 421 SRHLEWARELHNYMTPFVSKSPRAAYVNYKDLDMGTNNDDRVPTRYEEASVWGHRYFGNN 480
           SRHLEW RELH+YMTPFVSKSPRAAY+N KDLD+G NN+D +PT YEEAS+WGH+YFG N
Sbjct: 455 SRHLEWTRELHDYMTPFVSKSPRAAYINNKDLDIGRNNEDGIPTSYEEASIWGHQYFGIN 514

Query: 481 FERLMEVKTKVDPFNFFRHEQSIPPAPP 487
           FERL+EVKTK DPFNFFRHEQSIPP  P
Sbjct: 515 FERLVEVKTKADPFNFFRHEQSIPPVAP 541

BLAST of PI0014254 vs. NCBI nr
Match: XP_008463307.2 (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo] >TYK17150.1 tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 926.8 bits (2394), Expect = 7.8e-266
Identity = 458/511 (89.63%), Postives = 477/511 (93.35%), Query Frame = 0

Query: 1   MEDFVNCIYFHSSNNNDSNSTIIHTPSSSSYSYVLNFSIRNLRFSEPKLP---------- 60
           MEDFV+CIY HSSNNNDSNST+IHTPSSSSYSYVLNFSIRNLRFSEP+ P          
Sbjct: 1   MEDFVDCIYVHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPVAIITPSH 60

Query: 61  -----------NHHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDIEDETAWV 120
                        HGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITID+EDETAWV
Sbjct: 61  ASQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDVEDETAWV 120

Query: 121 QSGATVGELYYRIGEKSRTLGFPAGFTATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYV 180
           QSGATVGELYY+IGEKSRTLGFPAGF ATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYV
Sbjct: 121 QSGATVGELYYKIGEKSRTLGFPAGFKATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYV 180

Query: 181 VDGNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQNAA 240
           VD NGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQNAA
Sbjct: 181 VDRNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQNAA 240

Query: 241 NLIYRWQYIAPWVNQDLFISAWVTASNSS-DGSGRIMQASFFSLFLGNATELLSLMEKTF 300
           NLIYRWQYIAPWV+QDLFISAWVTASNSS D SGRIM+ASFFSLFLGNATELLSLMEKTF
Sbjct: 241 NLIYRWQYIAPWVDQDLFISAWVTASNSSKDESGRIMEASFFSLFLGNATELLSLMEKTF 300

Query: 301 PELGLKKEDCLEMSWVESMAFSASGFVSAKSLELLLDRTPLNNGRYKTKSDYATQPISET 360
           PELGLKK+DCLEMSWVESMAFSASGFV AKSLELLLDRTP++NGRYKTKSDYAT+PIS+T
Sbjct: 301 PELGLKKKDCLEMSWVESMAFSASGFVYAKSLELLLDRTPVDNGRYKTKSDYATEPISKT 360

Query: 361 ALEGMWERFKDEELETVQLILIPFGGKMNEISESETPFPHRAGYPIHISYYLTWQRPNAD 420
           AL+GMWERFKDEELETVQLILIPFGGKMNEISESETP PHRAGYPIHI YYLTWQ+PNAD
Sbjct: 361 ALKGMWERFKDEELETVQLILIPFGGKMNEISESETPSPHRAGYPIHIGYYLTWQQPNAD 420

Query: 421 SRHLEWARELHNYMTPFVSKSPRAAYVNYKDLDMGTNNDDRVPTRYEEASVWGHRYFGNN 480
           SR+LEWARELH+YMTPFVSKSPRAAYVNY+DLDMGTNNDD VPTRY+EAS+WGHRYFGNN
Sbjct: 421 SRNLEWARELHDYMTPFVSKSPRAAYVNYRDLDMGTNNDDGVPTRYKEASLWGHRYFGNN 480

Query: 481 FERLMEVKTKVDPFNFFRHEQSIPPAPPRVG 490
           FERLMEVK KVDPFNFFRHEQSIPPAPPRVG
Sbjct: 481 FERLMEVKMKVDPFNFFRHEQSIPPAPPRVG 511

BLAST of PI0014254 vs. NCBI nr
Match: XP_004147307.2 (berberine bridge enzyme-like 27 [Cucumis sativus])

HSP 1 Score: 924.9 bits (2389), Expect = 3.0e-265
Identity = 457/511 (89.43%), Postives = 472/511 (92.37%), Query Frame = 0

Query: 1   MEDFVNCIYFHSSNNNDSNSTIIHTPSSSSYSYVLNFSIRNLRFSEPKLP---------- 60
           M+DFVNCIY HSSNNNDSNST+IHTPSSSSYSYVLNFSIRNLRFSEP+ P          
Sbjct: 30  MKDFVNCIYVHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPVAIITPSH 89

Query: 61  -----------NHHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDIEDETAWV 120
                        HGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITID+EDE+AWV
Sbjct: 90  ASQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDVEDESAWV 149

Query: 121 QSGATVGELYYRIGEKSRTLGFPAGFTATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYV 180
           QSGATVGELY+RIGEKSRTLGFPAGF ATIGLGGFLSGGG GMMVRKYGLGADNVVDAYV
Sbjct: 150 QSGATVGELYFRIGEKSRTLGFPAGFAATIGLGGFLSGGGFGMMVRKYGLGADNVVDAYV 209

Query: 181 VDGNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQNAA 240
           VDGNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHK WDQNAA
Sbjct: 210 VDGNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKIWDQNAA 269

Query: 241 NLIYRWQYIAPWVNQDLFISAWVTASNSS-DGSGRIMQASFFSLFLGNATELLSLMEKTF 300
           NLIYRWQYIAPWV+QDLFISAWVTASNSS DGSGRIM+ASFFSLFLGNATELLSLMEKTF
Sbjct: 270 NLIYRWQYIAPWVDQDLFISAWVTASNSSHDGSGRIMEASFFSLFLGNATELLSLMEKTF 329

Query: 301 PELGLKKEDCLEMSWVESMAFSASGFVSAKSLELLLDRTPLNNGRYKTKSDYATQPISET 360
           PELGLKKEDCLE SWVESMAFSASGFVSAKSLELLLDRTPL+NGRYKTKSDYAT+PISET
Sbjct: 330 PELGLKKEDCLETSWVESMAFSASGFVSAKSLELLLDRTPLHNGRYKTKSDYATEPISET 389

Query: 361 ALEGMWERFKDEELETVQLILIPFGGKMNEISESETPFPHRAGYPIHISYYLTWQRPNAD 420
            LEGMWERFKDEELETVQLILIPFGGK NEISESETP PHRAGYPIHI YYLTWQRP+AD
Sbjct: 390 VLEGMWERFKDEELETVQLILIPFGGKTNEISESETPSPHRAGYPIHIGYYLTWQRPDAD 449

Query: 421 SRHLEWARELHNYMTPFVSKSPRAAYVNYKDLDMGTNNDDRVPTRYEEASVWGHRYFGNN 480
           S+HL+WARELHNYMTPFVSKSPRAAYVNY+DLDMGTNNDD VPTR EEAS+WGHRYFGNN
Sbjct: 450 SKHLKWARELHNYMTPFVSKSPRAAYVNYRDLDMGTNNDDGVPTRCEEASIWGHRYFGNN 509

Query: 481 FERLMEVKTKVDPFNFFRHEQSIPPAPPRVG 490
           FERLMEVK KVDPFNFFRHEQSIPPAP  VG
Sbjct: 510 FERLMEVKRKVDPFNFFRHEQSIPPAPTSVG 540

BLAST of PI0014254 vs. NCBI nr
Match: KAE8652747.1 (hypothetical protein Csa_013703 [Cucumis sativus])

HSP 1 Score: 924.9 bits (2389), Expect = 3.0e-265
Identity = 457/511 (89.43%), Postives = 472/511 (92.37%), Query Frame = 0

Query: 1   MEDFVNCIYFHSSNNNDSNSTIIHTPSSSSYSYVLNFSIRNLRFSEPKLP---------- 60
           M+DFVNCIY HSSNNNDSNST+IHTPSSSSYSYVLNFSIRNLRFSEP+ P          
Sbjct: 289 MKDFVNCIYVHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPVAIITPSH 348

Query: 61  -----------NHHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDIEDETAWV 120
                        HGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITID+EDE+AWV
Sbjct: 349 ASQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDVEDESAWV 408

Query: 121 QSGATVGELYYRIGEKSRTLGFPAGFTATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYV 180
           QSGATVGELY+RIGEKSRTLGFPAGF ATIGLGGFLSGGG GMMVRKYGLGADNVVDAYV
Sbjct: 409 QSGATVGELYFRIGEKSRTLGFPAGFAATIGLGGFLSGGGFGMMVRKYGLGADNVVDAYV 468

Query: 181 VDGNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQNAA 240
           VDGNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHK WDQNAA
Sbjct: 469 VDGNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKIWDQNAA 528

Query: 241 NLIYRWQYIAPWVNQDLFISAWVTASNSS-DGSGRIMQASFFSLFLGNATELLSLMEKTF 300
           NLIYRWQYIAPWV+QDLFISAWVTASNSS DGSGRIM+ASFFSLFLGNATELLSLMEKTF
Sbjct: 529 NLIYRWQYIAPWVDQDLFISAWVTASNSSHDGSGRIMEASFFSLFLGNATELLSLMEKTF 588

Query: 301 PELGLKKEDCLEMSWVESMAFSASGFVSAKSLELLLDRTPLNNGRYKTKSDYATQPISET 360
           PELGLKKEDCLE SWVESMAFSASGFVSAKSLELLLDRTPL+NGRYKTKSDYAT+PISET
Sbjct: 589 PELGLKKEDCLETSWVESMAFSASGFVSAKSLELLLDRTPLHNGRYKTKSDYATEPISET 648

Query: 361 ALEGMWERFKDEELETVQLILIPFGGKMNEISESETPFPHRAGYPIHISYYLTWQRPNAD 420
            LEGMWERFKDEELETVQLILIPFGGK NEISESETP PHRAGYPIHI YYLTWQRP+AD
Sbjct: 649 VLEGMWERFKDEELETVQLILIPFGGKTNEISESETPSPHRAGYPIHIGYYLTWQRPDAD 708

Query: 421 SRHLEWARELHNYMTPFVSKSPRAAYVNYKDLDMGTNNDDRVPTRYEEASVWGHRYFGNN 480
           S+HL+WARELHNYMTPFVSKSPRAAYVNY+DLDMGTNNDD VPTR EEAS+WGHRYFGNN
Sbjct: 709 SKHLKWARELHNYMTPFVSKSPRAAYVNYRDLDMGTNNDDGVPTRCEEASIWGHRYFGNN 768

Query: 481 FERLMEVKTKVDPFNFFRHEQSIPPAPPRVG 490
           FERLMEVK KVDPFNFFRHEQSIPPAP  VG
Sbjct: 769 FERLMEVKRKVDPFNFFRHEQSIPPAPTSVG 799

BLAST of PI0014254 vs. NCBI nr
Match: XP_038895067.1 (berberine bridge enzyme-like 18 [Benincasa hispida])

HSP 1 Score: 855.5 bits (2209), Expect = 2.2e-244
Identity = 423/509 (83.10%), Postives = 450/509 (88.41%), Query Frame = 0

Query: 1   MEDFVNCIYFHSSNNNDSNSTIIHTPSSSSYSYVLNFSIRNLRFSEPKLP---------- 60
           MEDFV+CIY H SNNNDSNST+IHTPSSSSYSYVLNFSIRNLRFSEP+ P          
Sbjct: 29  MEDFVHCIYLH-SNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPVAIITPSH 88

Query: 61  -----------NHHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDIEDETAWV 120
                        HG QIRTRSGGHDFEG SYVANVPFVLIDLINLNSITID EDE+AWV
Sbjct: 89  ASQVQAAVICCKSHGFQIRTRSGGHDFEGSSYVANVPFVLIDLINLNSITIDTEDESAWV 148

Query: 121 QSGATVGELYYRIGEKSRTLGFPAGFTATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYV 180
           QSGATVGELYYRIGEKSRTLGFPAGF  TIG+GGFLSGGG GMMVRKYGL ADNVVDAYV
Sbjct: 149 QSGATVGELYYRIGEKSRTLGFPAGFVPTIGIGGFLSGGGFGMMVRKYGLAADNVVDAYV 208

Query: 181 VDGNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQNAA 240
           VD NG V +RYSMGEDLFWAIRGGGGGSFGIVLAWKL+LV VP  VTSF+L KTWDQNAA
Sbjct: 209 VDANGSVFDRYSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPTVTSFSLSKTWDQNAA 268

Query: 241 NLIYRWQYIAPWVNQDLFISAWVTASNSSDGSGRIMQASFFSLFLGNATELLSLMEKTFP 300
           +LI+RWQY+AP V+QDLFISAWVTA NSSDGSGRIM+ASFFSL+LG ATELLSLMEKTFP
Sbjct: 269 DLIHRWQYVAPEVDQDLFISAWVTAFNSSDGSGRIMEASFFSLYLGKATELLSLMEKTFP 328

Query: 301 ELGLKKEDCLEMSWVESMAFSASGFVSAKSLELLLDRTPLNNGRYKTKSDYATQPISETA 360
           ELGLKKEDCLEMSWVESMAFSASGFVSAKSLELLL+R+PL+NGR K KSDY T+PISETA
Sbjct: 329 ELGLKKEDCLEMSWVESMAFSASGFVSAKSLELLLNRSPLDNGRQKIKSDYPTEPISETA 388

Query: 361 LEGMWERFKDEELETVQLILIPFGGKMNEISESETPFPHRAGYPIHISYYLTWQRPNADS 420
           LEGMWERFK+EELE VQ ILIPFGGKMNEISESETP  HRAGYPIHI YY+ WQ P+ADS
Sbjct: 389 LEGMWERFKNEELEKVQFILIPFGGKMNEISESETPSAHRAGYPIHIGYYVKWQHPDADS 448

Query: 421 RHLEWARELHNYMTPFVSKSPRAAYVNYKDLDMGTNNDDRVPTRYEEASVWGHRYFGNNF 480
           RHLEW REL+NYMTPFVSKSPRAAY NY+DLD+GTNN+DR+ T YEEASVWGH+YFGNNF
Sbjct: 449 RHLEWTRELYNYMTPFVSKSPRAAYANYRDLDIGTNNEDRILTSYEEASVWGHQYFGNNF 508

Query: 481 ERLMEVKTKVDPFNFFRHEQSIPPAPPRV 489
           ERLMEVK KVD FNFFRHEQSIPPAPPRV
Sbjct: 509 ERLMEVKAKVDQFNFFRHEQSIPPAPPRV 536

BLAST of PI0014254 vs. NCBI nr
Match: XP_038895033.1 (berberine bridge enzyme-like 18 [Benincasa hispida])

HSP 1 Score: 854.4 bits (2206), Expect = 4.9e-244
Identity = 420/510 (82.35%), Postives = 453/510 (88.82%), Query Frame = 0

Query: 1   MEDFVNCIYFHSSNNNDSNSTIIHTPSSSSYSYVLNFSIRNLRFSEPKLP---------- 60
           MEDFV+CI  H SNNNDSNST+IH PSSSSYSY+LNFSI N RFS+P+ P          
Sbjct: 30  MEDFVHCINLH-SNNNDSNSTLIHLPSSSSYSYILNFSICNRRFSDPETPKPVAIITPSH 89

Query: 61  -----------NHHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDIEDETAWV 120
                        HG QIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITI++EDE+AWV
Sbjct: 90  ASQVQATVICCKSHGFQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITINMEDESAWV 149

Query: 121 QSGATVGELYYRIGEKSRTLGFPAGFTATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYV 180
           QSGATVGELYYRIGEKS+TLGFPAGF   IG+GGFLSGGG+GMMVRKYGL ADNVVDAYV
Sbjct: 150 QSGATVGELYYRIGEKSQTLGFPAGFVPAIGVGGFLSGGGLGMMVRKYGLAADNVVDAYV 209

Query: 181 VDGNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQNAA 240
           VD NGRV NR+SMGEDLFWAIRGGGGGSFGIVLAWKL+LV VP IVTSFALHKTWDQN A
Sbjct: 210 VDENGRVFNRHSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFALHKTWDQNTA 269

Query: 241 NLIYRWQYIAPWVNQDLFISAWVTASNSSDGSGRIMQASFFSLFLGNATELLSLMEKTFP 300
           +LI+RWQY+AP V+QDLFI+AWVTASNSSDGSGRIM+ASFFSL+LG ATELLSLME+TFP
Sbjct: 270 HLIHRWQYVAPEVDQDLFITAWVTASNSSDGSGRIMEASFFSLYLGKATELLSLMERTFP 329

Query: 301 ELGLKKEDCLEMSWVESMAFSASGFVSAKSLELLLDRTPLNNGRYKTKSDYATQPISETA 360
           ELGLKKEDCLEMSWVESMAFSASGFVSAKSLELLL+RTPLNNGRYK KSDY T+PISETA
Sbjct: 330 ELGLKKEDCLEMSWVESMAFSASGFVSAKSLELLLNRTPLNNGRYKIKSDYLTEPISETA 389

Query: 361 LEGMWERFKDEELETVQLILIPFGGKMNEISESETPFPHRAGYPIHISYYLTWQRPNADS 420
           LEGMWERFK+EELE VQL+LIPFGGKMNEISESETPFPHRA YPI I Y +TWQRP+ADS
Sbjct: 390 LEGMWERFKNEELEKVQLVLIPFGGKMNEISESETPFPHRARYPIQIGYCVTWQRPDADS 449

Query: 421 RHLEWARELHNYMTPFVSKSPRAAYVNYKDLDMGTNNDDRVPTRYEEASVWGHRYFGNNF 480
           RHLEW REL++YMTPFVSKSPRA Y+N  DLD+GTNN D +PT YEEASVWGH+YFGNNF
Sbjct: 450 RHLEWTRELYSYMTPFVSKSPRAVYINNIDLDLGTNNKDGIPTSYEEASVWGHQYFGNNF 509

Query: 481 ERLMEVKTKVDPFNFFRHEQSIPPAPPRVG 490
           ERLMEVKTKVDPFNFFRHEQSIPP PPRVG
Sbjct: 510 ERLMEVKTKVDPFNFFRHEQSIPPTPPRVG 538

BLAST of PI0014254 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 464.9 bits (1195), Expect = 7.9e-131
Identity = 241/508 (47.44%), Postives = 329/508 (64.76%), Query Frame = 0

Query: 2   EDFVNCIYFHSSNNNDSNSTIIHTPSSSSYSYVLNFSIRNLRF----------------- 61
           E+F+ C+   S   N+ +S IIHT    SY  +LN SI+N RF                 
Sbjct: 35  ENFLKCL---SHRINEDDSRIIHTSKDPSYFSILNSSIQNPRFFVLETPKPVSIITPVQA 94

Query: 62  ----SEPKLPNHHGLQIRTRSGGHDFEGRSYVA-NVPFVLIDLINLNSITIDIEDETAWV 121
               S  K    HG+ IRTRSGGHD+EG SY+A + PFV+IDL NL SIT+D+++ T WV
Sbjct: 95  TDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITLDVDNRTGWV 154

Query: 122 QSGATVGELYYRIGEKSRTLGFPAGFTATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYV 181
           QSGAT+GELYY IG+ S++L FPAG   T+G+GG   GGG G ++RKYGL ADNV+DA++
Sbjct: 155 QSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLSADNVIDAHI 214

Query: 182 VDGNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQNAA 241
           VD NG  ++R  MGED FWAIRGGGG SF +VL+WK+RL+ VPS+VT F + KT ++ A 
Sbjct: 215 VDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKVVKTSEKEAV 274

Query: 242 NLIYRWQYIAPWVNQDLFISAWVTASNSSDGSGRIMQASFFSLFLGNATELLSLMEKTFP 301
           ++I +WQYIA  V  DLFI A +            + ASF  L+LG  ++LL+LM+  FP
Sbjct: 275 SIINKWQYIADKVPNDLFIRAMLQKETE-------VYASFPGLYLGPVSDLLALMKDKFP 334

Query: 302 ELGLKKEDCLEMSWVESMAFSASGFVSAKSLELLLDRTPLNNGRYKTKSDYATQPISETA 361
           ELGL+  +C EMSW+ES+ +    F+  +S+E+L  R   +   +K K D+  +PI +TA
Sbjct: 335 ELGLEIGNCREMSWIESVLW----FIKGESMEILAKRKRTSRS-FKGKDDFIEEPIPKTA 394

Query: 362 LEGMWERFKDEELETVQLILIPFGGKMNEISESETPFPHRAGYPIHISYYLTW-----QR 421
           ++ +W RF+  E    ++IL PFGGKM+EI+++E PFPHR G    I Y   W     + 
Sbjct: 395 IQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQYLAYWSEEEDKN 454

Query: 422 PNADSRHLEWARELHNYMTPFVSKSPRAAYVNYKDLDMGTNNDDRVPTRYEEASVWGHRY 481
                ++L W   ++ +MTP+VSKSPR AYVN++D+D+G      + T+YEEA VWG +Y
Sbjct: 455 KTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGLNMKTKYEEAKVWGVKY 514

Query: 482 FGNNFERLMEVKTKVDPFNFFRHEQSIP 483
           F NNF+RL+ VKT VDP +FF  EQSIP
Sbjct: 515 FKNNFDRLVRVKTNVDPMDFFCDEQSIP 527

BLAST of PI0014254 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 457.6 bits (1176), Expect = 1.3e-128
Identity = 237/503 (47.12%), Postives = 330/503 (65.61%), Query Frame = 0

Query: 4   FVNCIYFHSSNNNDSN--STIIHTPSSSSYSYVLNFSIRNLRFSEPKLP----------- 63
           F+ C+   S   NDSN  S +IHTP+ +S+S VL  SI+N RFS P +P           
Sbjct: 37  FLQCL---SLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLILTPVQP 96

Query: 64  ----------NHHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDIEDETAWVQ 123
                        G+ IRTRSGGHD+EG SYV + PFV++DL NL SIT+D+++ + WVQ
Sbjct: 97  SDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDNRSVWVQ 156

Query: 124 SGATVGELYYRIGEKSRTLGFPAGFTATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYVV 183
           +GAT+GELYY IG+K+RTL FPAG   T+G+GG  SGGG G ++RK+GL AD+V+DA VV
Sbjct: 157 TGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHVIDARVV 216

Query: 184 DGNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQNAAN 243
           D  GR++ R  MGED FWAIRGGGG SF +VL+WK+ L+ VPS VT F + K  +Q+A  
Sbjct: 217 DARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFSEQSALK 276

Query: 244 LIYRWQYIAPWVNQDLFISAWVTASNSSDGSGRIMQASFFSLFLGNATELLSLMEKTFPE 303
           +I+RWQ++A  V+ DLFI   +    +      +++ASF  L+LG+   LL ++ K FPE
Sbjct: 277 IIHRWQFVADKVSDDLFIRVMLQRYKN------MVRASFPGLYLGSVKNLLKMVNKEFPE 336

Query: 304 LGLKKEDCLEMSWVESMAFSASGFVSAKSLELLLDRTPLNNGRYKTKSDYATQPISETAL 363
           LGL+++DC EMSW+ES+ + A   +  + + +L  RT  +   +K KSD+  +P+ +TA+
Sbjct: 337 LGLEEDDCTEMSWIESVIWFAE--LGEEPINVLTKRTRASLA-FKAKSDFVQEPMPKTAI 396

Query: 364 EGMWERFKDEELETVQLILIPFGGKMNEISESETPFPHRAGYPIHISYYLTWQRPNADSR 423
             +W R ++ E E  QLI  PFGGKM+EI++ ETPFPHR G    I Y   W R +   +
Sbjct: 397 SKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNYW-RGDVKEK 456

Query: 424 HLEWARELHNYMTPFVSKSPRAAYVNYKDLDMGTNNDDRVPTRYEEASVWGHRYFGNNFE 483
           ++ W   +++ M+ FV+KSPR AY+N +DLD+G     +  ++YEE   WG +YF NNFE
Sbjct: 457 YMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVGVK-RSKYEEGKSWGVKYFKNNFE 516

BLAST of PI0014254 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 453.8 bits (1166), Expect = 1.8e-127
Identity = 237/510 (46.47%), Postives = 327/510 (64.12%), Query Frame = 0

Query: 2   EDFVNCIYFHSSNNNDSNSTIIHTPSSSSYSYVLNFSIRNLRFSEPKLPNH--------- 61
           EDF+ C+ +   N+N     +IHT   SS+  +L+ SI+N RFS  + P           
Sbjct: 29  EDFLKCLSYR-MNDNTVEPKVIHTSKDSSFFSILDSSIQNPRFSVSETPKPVSIITPVKA 88

Query: 62  ------------HGLQIRTRSGGHDFEGRSYVA-NVPFVLIDLINLNSITIDIEDETAWV 121
                       HG+ +RTRS GH +EG SY+A N PF +IDL NL SI++D+++ T WV
Sbjct: 89  SDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPFAVIDLRNLRSISLDVDNRTGWV 148

Query: 122 QSGATVGELYYRIGEKSRTLGFPAGFTATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYV 181
           Q+GAT GELYY IG+ +++L FPAG   T+G+GG  SGGG G ++RKYGL ADN++DA V
Sbjct: 149 QTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGYGTLLRKYGLAADNIIDALV 208

Query: 182 VDGNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQNAA 241
           VD +GR+++R +MGED FWAIRGGGG SFG++L+WK++LV VPS +T F + KT  + A 
Sbjct: 209 VDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVDVPSTITVFKVQKTSKKEAV 268

Query: 242 NLIYRWQYIAPWVNQDLFISAWVTASNSSDGSGRIMQASFFSLFLGNATELLSLMEKTFP 301
            +I +WQY A  V  DLFI   +  SN +      + A F  L++G    LL+LME+ FP
Sbjct: 269 RIIKKWQYAADKVPDDLFIRTTLERSNKN-----AVHALFTGLYIGPVNNLLALMEEKFP 328

Query: 302 ELGLKKEDCLEMSWVESMAFSASGFVSAKSLELLL--DRTPLNNGRYKTKSDYATQPISE 361
           ELGL+KE C EMSW+ES+ + A  F   +SL +L   +RT L+   +K K D+  +PI E
Sbjct: 329 ELGLEKEGCEEMSWIESVLWFAD-FPKGESLGVLTNRERTSLS---FKGKDDFVQEPIPE 388

Query: 362 TALEGMWERFKDEELETVQLILIPFGGKMNEISESETPFPHRAGYPIHISYYLTW----- 421
            A++ +W R +  E    ++IL PFGGKM+E++E ETPFPHR G    I Y   W     
Sbjct: 389 AAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPFPHRGGNLYEIQYVAYWREEED 448

Query: 422 QRPNADSRHLEWARELHNYMTPFVSKSPRAAYVNYKDLDMGTNNDDRVPTRYEEASVWGH 481
           +      ++L+W   ++ +MTP+VSKSPR AYVN+KD+D+G     +  T+YEE   WG 
Sbjct: 449 KNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMDLGMYLGKK-KTKYEEGKSWGV 508

Query: 482 RYFGNNFERLMEVKTKVDPFNFFRHEQSIP 483
           +YF NNFERL+ VKT+VDP +FF  EQSIP
Sbjct: 509 KYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of PI0014254 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 449.5 bits (1155), Expect = 3.4e-126
Identity = 231/507 (45.56%), Postives = 328/507 (64.69%), Query Frame = 0

Query: 2   EDFVNCIYFHSSNNNDSNSTII-HTPSSSSYSYVLNFSIRNLRFSEPKLPNHH------- 61
           +DFV C+  +S  +   +S+   H  ++SS+   L  S +NLR+  P  P          
Sbjct: 33  QDFVMCLVDNSDASFPMDSSFFTHDLNASSFKLALETSAQNLRYLMPSNPKPEFIFEPLY 92

Query: 62  --------------GLQIRTRSGGHDFEGRSYVANV--PFVLIDLINLNSITIDIEDETA 121
                          L +R RSGGHD+EG SYV+ +   FV++DL  L  I++DIE  +A
Sbjct: 93  ETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMETAFVIVDLSKLRQISVDIESNSA 152

Query: 122 WVQSGATVGELYYRIGEKSRTLGFPAGFTATIGLGGFLSGGGIGMMVRKYGLGADNVVDA 181
           WV +GA++GE+YYRI EKS+  GFPAG   ++G+GG + GG  G M+RK+GLGADNV+DA
Sbjct: 153 WVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHIIGGAYGSMMRKFGLGADNVLDA 212

Query: 182 YVVDGNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQN 241
            +VD +G+++NR +MGED+FWAIRGGGGGSFG++LAWK++LV VP IVT F + +T +Q+
Sbjct: 213 RIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPEIVTVFTVTRTLEQD 272

Query: 242 AANLIYRWQYIAPWVNQDLFISAWV-TASNSSDGSGRIMQASFFSLFLGNATELLSLMEK 301
              L+Y+WQ +A  +++DLFI   +   S +     R +  S+   FLG+A  LL +M++
Sbjct: 273 GTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSKERTISTSYQGQFLGDANRLLQVMQR 332

Query: 302 TFPELGLKKEDCLEMSWVESMAFSASGFVSAKSLELLLDRTPLNNGRYKTKSDYATQPIS 361
           +FP+LGL K+DCLE SW++S+ + A GF S    E LLD   L    +K KSDY  +PI 
Sbjct: 333 SFPQLGLTKKDCLETSWIKSVMYIA-GFPSTAPSEALLDGKSLFKNYFKAKSDYVEEPIP 392

Query: 362 ETALEGMWERFKDEELETVQLILIPFGGKMNEISESETPFPHRAGYPIHISYYLTWQ-RP 421
              LEG+WE+  +E  ++   I  P+GG M +I E+ETPFPHR+G    I +   WQ   
Sbjct: 393 VEGLEGLWEKLLEE--DSPLTIWNPYGGMMAKIPETETPFPHRSGTLFKIQWLTLWQDGK 452

Query: 422 NADSRHLEWARELHNYMTPFVSKSPRAAYVNYKDLDMGTNNDDRVPTRYEEASVWGHRYF 481
            ++++H+ W RE+++YM  +VSKSPR+AYVNY+DLD+G N       +  +A  WG+RYF
Sbjct: 453 TSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMNG------KGSDAREWGNRYF 512

Query: 482 GNNFERLMEVKTKVDPFNFFRHEQSIP 483
             NFERL+E+K K DP NFFRHEQSIP
Sbjct: 513 KGNFERLVEIKAKFDPENFFRHEQSIP 530

BLAST of PI0014254 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 444.5 bits (1142), Expect = 1.1e-124
Identity = 234/509 (45.97%), Postives = 330/509 (64.83%), Query Frame = 0

Query: 2   EDFVNCIYFHSSNNNDSNSTII-HTPSSSSYSYVLNFSIRNLRFSEPKLPN--------H 61
           + F+NC+  ++        T    T + S +S VL  + +NLRF +  +P         H
Sbjct: 33  DQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLESTAQNLRFLKKSMPKPGFIFSPIH 92

Query: 62  HG-------------LQIRTRSGGHDFEGRSYVANV--PFVLIDLINLNSITIDIEDETA 121
                          + +R RSGGHD+EG SYV+ +  PF+L+DL  +  + I+I+D +A
Sbjct: 93  ESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKPFILMDLSKMRQVNINIQDNSA 152

Query: 122 WVQSGATVGELYYRIGEKSRTLGFPAGFTATIGLGGFLSGGGIGMMVRKYGLGADNVVDA 181
           WVQSGATVGELYYRI EKS+  GFPAG  +++G+GG ++GG  G M+RKYGLGADNV+DA
Sbjct: 153 WVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHITGGAYGSMMRKYGLGADNVLDA 212

Query: 182 YVVDGNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQN 241
            +VD NG++++R +MGED FWAIRGG GGSFGI+LAWK++LV VP  VT F + KT  Q+
Sbjct: 213 KIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKIKLVPVPKTVTVFTVTKTLQQD 272

Query: 242 AAN-LIYRWQYIAPWVNQDLFISAWVTASNSSDGSGRIMQASFFSLFLGNATELLSLMEK 301
             N +I +WQ +A  + ++LFI   V  + +  G  + +  S+ +LFLG    L+++M+K
Sbjct: 273 VGNKIISKWQRVADKLVEELFIR--VLFNVAGTGGNKTVTTSYNALFLGGKGTLMNVMKK 332

Query: 302 TFPELGLKKEDCLEMSWVESMAFSASGFVSAKSLELLLD-RTPLNNGRYKTKSDYATQPI 361
           +FPELGL  +DC+EMSW+ES+A+  SGF +     +LL  ++P     +K KSD+   PI
Sbjct: 333 SFPELGLTFKDCIEMSWLESIAY-ISGFPTHTPTNVLLQGKSPFPKVSFKAKSDFVKTPI 392

Query: 362 SETALEGMWERFKDEELETVQLILIPFGGKMNEISESETPFPHRAGYPIHISYYLTW-QR 421
            E+ L+G++++   E++    +I  P+GG M +I ES+ PFPHR G    + Y  +W   
Sbjct: 393 PESGLQGIFKKLLKEDIPL--MIWNPYGGMMAKIPESQIPFPHRKGVLFKVQYVTSWLDS 452

Query: 422 PNADSRHLEWARELHNYMTPFVSKSPRAAYVNYKDLDMGTNNDDRVPTRYEEASVWGHRY 481
               SRH+ W R+L++YMTP+VS +PR AYVNY+DLD+G N  D V T  ++A VWG  Y
Sbjct: 453 DKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGRNTKD-VKTCIKQAQVWGANY 512

Query: 482 FGNNFERLMEVKTKVDPFNFFRHEQSIPP 484
           F NNF RLM +K KVDP NFFRHEQSIPP
Sbjct: 513 FKNNFNRLMMIKAKVDPENFFRHEQSIPP 535