MS004278 (gene) Bitter gourd (TR) v1

BLAST of MS004278 vs. NCBI nr
Match: KAG6601125.1 (Aspartyl protease AED3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 718.0 bits (1852), Expect = 4.8e-203
Identity = 358/407 (87.96%), Postives = 384/407 (94.35%), Query Frame = 0

Query: 24  AAADDSATLKVFHV----SSVLKSKPLSWAENVVQMLRKDQARLQFLSSLVARRSVAPIA 83
           AAAD S+TL+VFH+    S    SKPLSWA+NV+QM  KDQARLQFLSSLVAR+SVAPIA
Sbjct: 35  AAADRSSTLQVFHIFSPCSPFRPSKPLSWADNVLQMQAKDQARLQFLSSLVARKSVAPIA 94

Query: 84  SARQLIQSPTYVVRAKIGTPAQTLLMALDTSNDAAWIPCSGCLGCPSATVFSSDKSSSFK 143
           SARQLIQSPT+VVRAKIGTPAQTLLMALDTSNDAAWIPCSGC+GCPS TVFSSDKSSSF+
Sbjct: 95  SARQLIQSPTFVVRAKIGTPAQTLLMALDTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFR 154

Query: 144 PLPCNSPQCNQVPNPSCGGSACGFNLTYGSSTVAADLVQDNITLANDSVPAYTFGCITKA 203
           PLPC SPQCNQVPNPSC  SACGFNLTYGSSTVAADLVQDNITLANDSVPAYTFGCITKA
Sbjct: 155 PLPCRSPQCNQVPNPSCSSSACGFNLTYGSSTVAADLVQDNITLANDSVPAYTFGCITKA 214

Query: 204 TGASVPPQGLLGLGRGPLSLLSQTQNLYQSTFSYCLPSFKSPNFSGSLRLGPVAQPKRIK 263
           TG+SVPPQGLLGLGRGPLSLL Q+Q+LY+STFSYCLPSFKS  FSGSLRLGPVAQPKRIK
Sbjct: 215 TGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFSYCLPSFKSTGFSGSLRLGPVAQPKRIK 274

Query: 264 YTPLLRNPRRSSLYYVNLIAVRVGRRVVDIPPSAFAFNPTTGSGTIIDSGTVFTRLVAPA 323
           YTPLLRNPRRSSLYYVNLIA+RVGRR+VDIPPSA AFN  TG+GTIIDSGT FTRLVAPA
Sbjct: 275 YTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSALAFNSATGAGTIIDSGTTFTRLVAPA 334

Query: 324 YTAVRDEFRRRVGNATVSPLGGFDTCYTVPIVSPTITFMFAGMNVTLPPDNFLIHSTAGS 383
           YTAVR+EFRRR+GNAT+S LGGFDTCYTVPI+SPTITFMFAGMNVTLPPDNFL+HS+AG+
Sbjct: 335 YTAVRNEFRRRIGNATISSLGGFDTCYTVPIISPTITFMFAGMNVTLPPDNFLLHSSAGT 394

Query: 384 TTCLAMAAAPDNVNSVLNVIANMQQQNHRLLFDIPNSRLGVAREPCS 427
           TTCLAMAAAPDNVNSVLNVIA+MQQQNHR+LFDIPNSR+GVAREPCS
Sbjct: 395 TTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDIPNSRIGVAREPCS 441

BLAST of MS004278 vs. NCBI nr
Match: XP_022957241.1 (aspartyl protease AED3 [Cucurbita moschata])

HSP 1 Score: 717.6 bits (1851), Expect = 6.3e-203
Identity = 357/407 (87.71%), Postives = 384/407 (94.35%), Query Frame = 0

Query: 24  AAADDSATLKVFHV----SSVLKSKPLSWAENVVQMLRKDQARLQFLSSLVARRSVAPIA 83
           AAAD S+TL+VFH+    S    SKPLSWA+NV+QM  KDQARLQFLSSLVAR+SVAPIA
Sbjct: 31  AAADRSSTLQVFHIFSPCSPFRPSKPLSWADNVLQMQAKDQARLQFLSSLVARKSVAPIA 90

Query: 84  SARQLIQSPTYVVRAKIGTPAQTLLMALDTSNDAAWIPCSGCLGCPSATVFSSDKSSSFK 143
           SARQLIQSPT+VVRAKIGTPAQTLLMALDTSNDAAWIPCSGC+GCPS TVFSSDKSSSF+
Sbjct: 91  SARQLIQSPTFVVRAKIGTPAQTLLMALDTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFR 150

Query: 144 PLPCNSPQCNQVPNPSCGGSACGFNLTYGSSTVAADLVQDNITLANDSVPAYTFGCITKA 203
           PLPC SPQCNQVPNPSC  SACGFNLTYGSSTVAADLVQDNITLANDSVPAYTFGCITKA
Sbjct: 151 PLPCRSPQCNQVPNPSCSSSACGFNLTYGSSTVAADLVQDNITLANDSVPAYTFGCITKA 210

Query: 204 TGASVPPQGLLGLGRGPLSLLSQTQNLYQSTFSYCLPSFKSPNFSGSLRLGPVAQPKRIK 263
           TG+SVPPQGLLGLGRGPLSLL Q+Q+LY+STFSYCLPSFKS  FSGSLRLGPVAQPKRIK
Sbjct: 211 TGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFSYCLPSFKSTGFSGSLRLGPVAQPKRIK 270

Query: 264 YTPLLRNPRRSSLYYVNLIAVRVGRRVVDIPPSAFAFNPTTGSGTIIDSGTVFTRLVAPA 323
           YTPLLRNPRRSSLYYVNLIA+RVGRR+VDIPPSA AFN  TG+GTIIDSGT FTRLVAPA
Sbjct: 271 YTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSALAFNSATGAGTIIDSGTTFTRLVAPA 330

Query: 324 YTAVRDEFRRRVGNATVSPLGGFDTCYTVPIVSPTITFMFAGMNVTLPPDNFLIHSTAGS 383
           YTAVR+EFRRR+GNAT+S LGGFDTCYTVPI+SPTITFMFAGMNVTLPPDNFL+HS+AG+
Sbjct: 331 YTAVRNEFRRRIGNATISSLGGFDTCYTVPIISPTITFMFAGMNVTLPPDNFLLHSSAGT 390

Query: 384 TTCLAMAAAPDNVNSVLNVIANMQQQNHRLLFDIPNSRLGVAREPCS 427
           TTCLAMAAAPDNVNSVLNVIA+MQQQNHR+LFD+PNSR+GVAREPCS
Sbjct: 391 TTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDVPNSRIGVAREPCS 437

BLAST of MS004278 vs. NCBI nr
Match: XP_008446966.1 (PREDICTED: aspartyl protease AED3 [Cucumis melo] >KAA0034764.1 aspartyl protease AED3 [Cucumis melo var. makuwa] >TYK09318.1 aspartyl protease AED3 [Cucumis melo var. makuwa])

HSP 1 Score: 716.5 bits (1848), Expect = 1.4e-202
Identity = 368/442 (83.26%), Postives = 395/442 (89.37%), Query Frame = 0

Query: 2   NNPHSHFLPLTVVVVLLLHCAAAAA------------DDSATLKVFHV----SSVLKSKP 61
           NN  S FLPL ++++LLL  + + A            D S+TLKVFH+    S    SKP
Sbjct: 2   NNTKSPFLPLPLLLLLLLLFSISTAKSHIPLNCNPADDRSSTLKVFHIFSPCSPFRPSKP 61

Query: 62  LSWAENVVQMLRKDQARLQFLSSLVARRSVAPIASARQLIQSPTYVVRAKIGTPAQTLLM 121
           LSWA+NV+QM  KDQARLQFLSSLVARRSV PIASARQLIQSPT+VVRAKIGTPAQTLL+
Sbjct: 62  LSWADNVLQMQAKDQARLQFLSSLVARRSVVPIASARQLIQSPTFVVRAKIGTPAQTLLL 121

Query: 122 ALDTSNDAAWIPCSGCLGCPSATVFSSDKSSSFKPLPCNSPQCNQVPNPSCGGSACGFNL 181
           ALDTSNDAAWIPCSGC+GCPS TVFSSDKSSSF+PLPC SPQCNQVPNPSC G+ACGFNL
Sbjct: 122 ALDTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCQSPQCNQVPNPSCSGTACGFNL 181

Query: 182 TYGSSTVAADLVQDNITLANDSVPAYTFGCITKATGASVPPQGLLGLGRGPLSLLSQTQN 241
           TYGSSTVAADLVQDN+TLA DSVPAYTFGCI KATG+SVPPQGLLGLGRGPLSLL Q+Q+
Sbjct: 182 TYGSSTVAADLVQDNVTLATDSVPAYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQS 241

Query: 242 LYQSTFSYCLPSFKSPNFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAVRVGRR 301
           LY+STFSYCLPSFKS NFSGSLRLGPVAQP RIKYTPLLRNPRRSSLYYVNLIA+RVGRR
Sbjct: 242 LYRSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLIAIRVGRR 301

Query: 302 VVDIPPSAFAFNPTTGSGTIIDSGTVFTRLVAPAYTAVRDEFRRRVG-NATVSPLGGFDT 361
           +VDIPPSA AFN  TG+GTIIDSGT FTRLVAPAYTAVRDEFRRRVG N TVS LGGFDT
Sbjct: 302 IVDIPPSALAFNSATGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRNVTVSSLGGFDT 361

Query: 362 CYTVPIVSPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIANMQQ 421
           CYTVPI+SPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIA+MQQ
Sbjct: 362 CYTVPIISPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQ 421

Query: 422 QNHRLLFDIPNSRLGVAREPCS 427
           QNHR+LFDIPNSR+GVAREPCS
Sbjct: 422 QNHRILFDIPNSRVGVAREPCS 443

BLAST of MS004278 vs. NCBI nr
Match: XP_023526864.1 (aspartyl protease AED3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 715.3 bits (1845), Expect = 3.1e-202
Identity = 355/407 (87.22%), Postives = 384/407 (94.35%), Query Frame = 0

Query: 24  AAADDSATLKVFHV----SSVLKSKPLSWAENVVQMLRKDQARLQFLSSLVARRSVAPIA 83
           AAAD S+TL+VFH+    S    SKPLSWA+NV+QM  KDQARLQFLSSLVAR+SVAPI+
Sbjct: 36  AAADRSSTLQVFHIFSPCSPFRPSKPLSWADNVLQMQAKDQARLQFLSSLVARKSVAPIS 95

Query: 84  SARQLIQSPTYVVRAKIGTPAQTLLMALDTSNDAAWIPCSGCLGCPSATVFSSDKSSSFK 143
           SARQLIQSPT+VVRAKIGTPAQTLLMALDTSNDAAWIPCSGC+GCPS TVFSSDKSSSF+
Sbjct: 96  SARQLIQSPTFVVRAKIGTPAQTLLMALDTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFR 155

Query: 144 PLPCNSPQCNQVPNPSCGGSACGFNLTYGSSTVAADLVQDNITLANDSVPAYTFGCITKA 203
           PLPC SPQCNQVPNPSC  S+CGFNLTYGSSTVAADLVQDNITLANDSVPAYTFGCITKA
Sbjct: 156 PLPCRSPQCNQVPNPSCSSSSCGFNLTYGSSTVAADLVQDNITLANDSVPAYTFGCITKA 215

Query: 204 TGASVPPQGLLGLGRGPLSLLSQTQNLYQSTFSYCLPSFKSPNFSGSLRLGPVAQPKRIK 263
           TG+SVPPQGLLGLGRGPLSLL Q+Q+LY+STFSYCLPSFKS  FSGSLRLGPVAQPKRIK
Sbjct: 216 TGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFSYCLPSFKSTGFSGSLRLGPVAQPKRIK 275

Query: 264 YTPLLRNPRRSSLYYVNLIAVRVGRRVVDIPPSAFAFNPTTGSGTIIDSGTVFTRLVAPA 323
           YTPLLRNPRRSSLYYVNLIA+RVGRR+VDIPPSA AFN  TG+GTIIDSGT FTRLVAPA
Sbjct: 276 YTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSALAFNSATGAGTIIDSGTTFTRLVAPA 335

Query: 324 YTAVRDEFRRRVGNATVSPLGGFDTCYTVPIVSPTITFMFAGMNVTLPPDNFLIHSTAGS 383
           YTAVR+EFRRR+GNAT+S LGGFDTCYTVPI+SPTITFMFAGMNVTLPPDNFL+HS+AG+
Sbjct: 336 YTAVRNEFRRRIGNATISSLGGFDTCYTVPIISPTITFMFAGMNVTLPPDNFLLHSSAGT 395

Query: 384 TTCLAMAAAPDNVNSVLNVIANMQQQNHRLLFDIPNSRLGVAREPCS 427
           TTCLAMAAAPDNVNSVLNVIA+MQQQNHR+LFD+PNSR+GVAREPCS
Sbjct: 396 TTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDVPNSRIGVAREPCS 442

BLAST of MS004278 vs. NCBI nr
Match: XP_022988941.1 (aspartyl protease AED3-like [Cucurbita maxima])

HSP 1 Score: 713.8 bits (1841), Expect = 9.1e-202
Identity = 355/407 (87.22%), Postives = 384/407 (94.35%), Query Frame = 0

Query: 24  AAADDSATLKVFHV----SSVLKSKPLSWAENVVQMLRKDQARLQFLSSLVARRSVAPIA 83
           AAAD S+TL+VFH+    S    SKPLSWA+NV+QM  KDQARLQFLSSLVA +SVAPIA
Sbjct: 33  AAADRSSTLQVFHIFSPCSPFRPSKPLSWADNVLQMQAKDQARLQFLSSLVACKSVAPIA 92

Query: 84  SARQLIQSPTYVVRAKIGTPAQTLLMALDTSNDAAWIPCSGCLGCPSATVFSSDKSSSFK 143
           SARQLIQSPT+VVRAKIGTPAQTLLMALDTSNDAAWIPCSGC+GCPS TVFSSDKS+SF+
Sbjct: 93  SARQLIQSPTFVVRAKIGTPAQTLLMALDTSNDAAWIPCSGCVGCPSTTVFSSDKSTSFR 152

Query: 144 PLPCNSPQCNQVPNPSCGGSACGFNLTYGSSTVAADLVQDNITLANDSVPAYTFGCITKA 203
           PLPC SPQCNQVPNPSC  SACGFNLTYGSSTVAADLVQDNITLANDSVPAYTFGCITKA
Sbjct: 153 PLPCRSPQCNQVPNPSCSSSACGFNLTYGSSTVAADLVQDNITLANDSVPAYTFGCITKA 212

Query: 204 TGASVPPQGLLGLGRGPLSLLSQTQNLYQSTFSYCLPSFKSPNFSGSLRLGPVAQPKRIK 263
           TG+SVPPQGLLGLGRGPLSLL Q+Q+LY+STFSYCLPSFKS +FSGSLRLGPVAQPKRIK
Sbjct: 213 TGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFSYCLPSFKSTSFSGSLRLGPVAQPKRIK 272

Query: 264 YTPLLRNPRRSSLYYVNLIAVRVGRRVVDIPPSAFAFNPTTGSGTIIDSGTVFTRLVAPA 323
           YTPLLRNPRRSSLYYVNLIA+RVGRR+VDIPPSA AFN  TG+GTIIDSGT FTRLVAPA
Sbjct: 273 YTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSALAFNSATGAGTIIDSGTTFTRLVAPA 332

Query: 324 YTAVRDEFRRRVGNATVSPLGGFDTCYTVPIVSPTITFMFAGMNVTLPPDNFLIHSTAGS 383
           YTAVR+EFRRR+GNAT+S LGGFDTCYTVPI+SPTITFMFAGMNVTLPPDNFL+HS+AG+
Sbjct: 333 YTAVRNEFRRRIGNATISSLGGFDTCYTVPIISPTITFMFAGMNVTLPPDNFLLHSSAGT 392

Query: 384 TTCLAMAAAPDNVNSVLNVIANMQQQNHRLLFDIPNSRLGVAREPCS 427
           TTCLAMAAAPDNVNSVLNVIA+MQQQNHR+LFD+PNSR+GVAREPCS
Sbjct: 393 TTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDVPNSRMGVAREPCS 439

BLAST of MS004278 vs. ExPASy Swiss-Prot
Match: O04496 (Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 1.2e-111
Identity = 223/437 (51.03%), Postives = 289/437 (66.13%), Query Frame = 0

Query: 9   LPLTVVVVLLLHCAAAAADDSATLKVFHVSSVLKSKPL-------SWAENVVQMLRKDQA 68
           LP T        CA AA D S  L +  +++  K  P        S  + V+ M   D  
Sbjct: 16  LPFTFTTATRDTCATAAPDGSDDLSIIPINA--KCSPFAPTHVSASVIDTVLHMASSDSH 75

Query: 69  RLQFLSSLVA---RRSVAPIASARQLIQSPTYVVRAKIGTPAQTLLMALDTSNDAAWIPC 128
           RL +LSSLVA   + +  P+AS  QL     YVVRAK+GTP Q + M LDTSNDA W+PC
Sbjct: 76  RLTYLSSLVAGKPKPTSVPVASGNQL-HIGNYVVRAKLGTPPQLMFMVLDTSNDAVWLPC 135

Query: 129 SGCLGCPSA-TVFSSDKSSSFKPLPCNSPQCNQV-----PNPSCGGSACGFNLTY-GSST 188
           SGC GC +A T F+++ SS++  + C++ QC Q      P+ S   S C FN +Y G S+
Sbjct: 136 SGCSGCSNASTSFNTNSSSTYSTVSCSTAQCTQARGLTCPSSSPQPSVCSFNQSYGGDSS 195

Query: 189 VAADLVQDNITLANDSVPAYTFGCITKATGASVPPQGLLGLGRGPLSLLSQTQNLYQSTF 248
            +A LVQD +TLA D +P ++FGCI  A+G S+PPQGL+GLGRGP+SL+SQT +LY   F
Sbjct: 196 FSASLVQDTLTLAPDVIPNFSFGCINSASGNSLPPQGLMGLGRGPMSLVSQTTSLYSGVF 255

Query: 249 SYCLPSFKSPNFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAVRVGRRVVDIPP 308
           SYCLPSF+S  FSGSL+LG + QPK I+YTPLLRNPRR SLYYVNL  V VG   V + P
Sbjct: 256 SYCLPSFRSFYFSGSLKLGLLGQPKSIRYTPLLRNPRRPSLYYVNLTGVSVGSVQVPVDP 315

Query: 309 SAFAFNPTTGSGTIIDSGTVFTRLVAPAYTAVRDEFRRRVGNATVSPLGGFDTCYTV--P 368
               F+  +G+GTIIDSGTV TR   P Y A+RDEFR++V  ++ S LG FDTC++    
Sbjct: 316 VYLTFDANSGAGTIIDSGTVITRFAQPVYEAIRDEFRKQVNVSSFSTLGAFDTCFSADNE 375

Query: 369 IVSPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIANMQQQNHRL 427
            V+P IT     +++ LP +N LIHS+AG+ TCL+MA    N N+VLNVIAN+QQQN R+
Sbjct: 376 NVAPKITLHMTSLDLKLPMENTLIHSSAGTLTCLSMAGIRQNANAVLNVIANLQQQNLRI 435

BLAST of MS004278 vs. ExPASy Swiss-Prot
Match: Q6F4N5 (Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 4.2e-101
Identity = 213/440 (48.41%), Postives = 284/440 (64.55%), Query Frame = 0

Query: 12  TVVVVLLLHCAAAAADDSATLKVFHVSSVLKSKPLSWAENVVQMLRKDQARLQFLSSLVA 71
           T + +LLL  AA  A  +A L V+H  +V  S P S  E+++ + R D ARL FLSS  A
Sbjct: 5   TTIPLLLLLLAATVAAAAAELSVYH--NVHPSSP-SPLESIIALARDDDARLLFLSSKAA 64

Query: 72  RRSV--APIASARQLIQSPTYVVRAKIGTPAQTLLMALDTSNDAAWIPCSGCLGCPSATV 131
              V  AP+AS +     P+YVVRA +G+P+Q LL+ALDTS DA W  CS C  CPS+++
Sbjct: 65  TAGVSSAPVASGQ---APPSYVVRAGLGSPSQQLLLALDTSADATWAHCSPCGTCPSSSL 124

Query: 132 FSSDKSSSFKPLPCNSPQC-----NQVPNPSCGGSA---------CGFNLTYGSSTVAAD 191
           F+   SSS+  LPC+S  C        P P  GG A         C F+  +  ++  A 
Sbjct: 125 FAPANSSSYASLPCSSSWCPLFQGQACPAPQGGGDAAPPPATLPTCAFSKPFADASFQAA 184

Query: 192 LVQDNITLANDSVPAYTFGCITKATG--ASVPPQGLLGLGRGPLSLLSQTQNLYQSTFSY 251
           L  D + L  D++P YTFGC++  TG   ++P QGLLGLGRGP++LLSQ  +LY   FSY
Sbjct: 185 LASDTLRLGKDAIPNYTFGCVSSVTGPTTNMPRQGLLGLGRGPMALLSQAGSLYNGVFSY 244

Query: 252 CLPSFKSPNFSGSLRLGP-VAQPKRIKYTPLLRNPRRSSLYYVNLIAVRVGRRVVDIPPS 311
           CLPS++S  FSGSLRLG    QP+ ++YTP+LRNP RSSLYYVN+  + VG   V +P  
Sbjct: 245 CLPSYRSYYFSGSLRLGAGGGQPRSVRYTPMLRNPHRSSLYYVNVTGLSVGHAWVKVPAG 304

Query: 312 AFAFNPTTGSGTIIDSGTVFTRLVAPAYTAVRDEFRRRVGNAT-VSPLGGFDTCYTVPIV 371
           +FAF+  TG+GT++DSGTV TR  AP Y A+R+EFRR+V   +  + LG FDTC+    V
Sbjct: 305 SFAFDAATGAGTVVDSGTVITRWTAPVYAALREEFRRQVAAPSGYTSLGAFDTCFNTDEV 364

Query: 372 ----SPTIT-FMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIANMQQQN 427
               +P +T  M  G+++ LP +N LIHS+A    CLAMA AP NVNSV+NVIAN+QQQN
Sbjct: 365 AAGGAPAVTVHMDGGVDLALPMENTLIHSSATPLACLAMAEAPQNVNSVVNVIANLQQQN 424

BLAST of MS004278 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 7.5e-50
Identity = 137/426 (32.16%), Postives = 211/426 (49.53%), Query Frame = 0

Query: 26  ADDSATLKVFHVSSVLKSKPLSWAENVVQMLRKDQARLQFLSSLVAR---RSVA----PI 85
           +  S TL + H+ ++  +K     E     L++D  R++ +++L A+   R+V     P 
Sbjct: 68  SSSSITLNLDHIDALSSNKTPD--ELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAPRPG 127

Query: 86  ASARQLIQ-----SPTYVVRAKIGTPAQTLLMALDTSNDAAWIPCSGCLGCPSAT--VFS 145
             +  ++      S  Y  R  +GTPA+ + M LDT +D  W+ C+ C  C S +  +F 
Sbjct: 128 GFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFD 187

Query: 146 SDKSSSFKPLPCNSPQCNQVPNPSCG--GSACGFNLTYG-SSTVAADLVQDNITLANDSV 205
             KS ++  +PC+SP C ++ +  C      C + ++YG  S    D   + +T   + V
Sbjct: 188 PRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRV 247

Query: 206 PAYTFGCITKATGASVPPQGLLGLGRGPLSLLSQTQNLYQSTFSYCLPSFKSPNFSGSLR 265
                GC     G  V   GLLGLG+G LS   QT + +   FSYCL    + +   S+ 
Sbjct: 248 KGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVV 307

Query: 266 LGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAVRV-GRRVVDIPPSAFAFNPTTGSGTIID 325
            G  A  +  ++TPLL NP+  + YYV L+ + V G RV  +  S F  +     G IID
Sbjct: 308 FGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIID 367

Query: 326 SGTVFTRLVAPAYTAVRDEFRRRVGNATVS---PLGGFDTCYTV----PIVSPTITFMFA 385
           SGT  TRL+ PAY A+RD F  RVG  T+        FDTC+ +     +  PT+   F 
Sbjct: 368 SGTSVTRLIRPAYIAMRDAF--RVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFR 427

Query: 386 GMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIANMQQQNHRLLFDIPNSRLGV 427
           G +V+LP  N+LI        C A A         L++I N+QQQ  R+++D+ +SR+G 
Sbjct: 428 GADVSLPATNYLIPVDTNGKFCFAFAGTMGG----LSIIGNIQQQGFRVVYDLASSRVGF 485

BLAST of MS004278 vs. ExPASy Swiss-Prot
Match: Q9LEW3 (Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 9.5e-45
Identity = 134/423 (31.68%), Postives = 206/423 (48.70%), Query Frame = 0

Query: 22  AAAAADDSATLKVFHVSSVLKSKPLSWAENVVQMLRKDQARLQFLSSLVARRSVAPIASA 81
           ++ A++  ++L+V H+             +  +++R+DQAR++ + S +++ S   ++ A
Sbjct: 55  SSKASNTKSSLRVVHMHGACSHLSSDARVDHDEIIRRDQARVESIYSKLSKNSANEVSEA 114

Query: 82  RQ---------LIQSPTYVVRAKIGTPAQTLLMALDTSNDAAWIPCSGCLG-CPS--ATV 141
           +           + S  Y+V   IGTP   L +  DT +D  W  C  CLG C S     
Sbjct: 115 KSTELPAKSGITLGSGNYIVTIGIGTPKHDLSLVFDTGSDLTWTQCEPCLGSCYSQKEPK 174

Query: 142 FSSDKSSSFKPLPCNSPQCNQVPNPSCGGSACGFNLTYG-SSTVAADLVQDNITLAN-DS 201
           F+   SS+++ + C+SP C      SC  S C +++ YG  S     L ++  TL N D 
Sbjct: 175 FNPSSSSTYQNVSCSSPMCEDA--ESCSASNCVYSIVYGDKSFTQGFLAKEKFTLTNSDV 234

Query: 202 VPAYTFGCITKATGASVPPQGLLGLGRGPLSLLSQTQNLYQSTFSYCLPSFKSPNFSGSL 261
           +    FGC     G      GLLGLG G LSL +QT   Y + FSYCLPSF S N +G L
Sbjct: 235 LEDVYFGCGENNQGLFDGVAGLLGLGPGKLSLPAQTTTTYNNIFSYCLPSFTS-NSTGHL 294

Query: 262 RLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAVRVGRRVVDIPPSAFAFNPTTGSGTIID 321
             G     + +K+TP+   P   + Y +++I + VG + + I P++F     +  G IID
Sbjct: 295 TFGSAGISESVKFTPISSFPSAFN-YGIDIIGISVGDKELAITPNSF-----STEGAIID 354

Query: 322 SGTVFTRLVAPAYTAVRDEFRRRVGN-ATVSPLGGFDTCYTV----PIVSPTITFMFAGM 381
           SGTVFTRL    Y  +R  F+ ++ +  + S  G FDTCY       +  PTI F FAG 
Sbjct: 355 SGTVFTRLPTKVYAELRSVFKEKMSSYKSTSGYGLFDTCYDFTGLDTVTYPTIAFSFAGS 414

Query: 382 NVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIANMQQQNHRLLFDIPNSRLGVAR 426
            V     + +      S  CLA A   D    +  +  N+QQ    +++D+   R+G A 
Sbjct: 415 TVVELDGSGISLPIKISQVCLAFAGNDD----LPAIFGNVQQTTLDVVYDVAGGRVGFAP 464

BLAST of MS004278 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 9.5e-45
Identity = 118/348 (33.91%), Postives = 166/348 (47.70%), Query Frame = 0

Query: 87  SPTYVVRAKIGTPAQTLLMALDTSNDAAWIPCSGCLGC--PSATVFSSDKSSSFKPLPCN 146
           S  Y VR  +G+P +   M +D+ +D  W+ C  C  C   S  VF   KS S+  + C 
Sbjct: 128 SGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSGSYTGVSCG 187

Query: 147 SPQCNQVPNPSCGGSACGFNLTYG-SSTVAADLVQDNITLANDSVPAYTFGCITKATGAS 206
           S  C+++ N  C    C + + YG  S     L  + +T A   V     GC  +  G  
Sbjct: 188 SSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTFAKTVVRNVAMGCGHRNRGMF 247

Query: 207 VPPQGLLGLGRGPLSLLSQTQNLYQSTFSYCLPSFKSPNFSGSLRLGPVAQPKRIKYTPL 266
           +   GLLG+G G +S + Q        F YCL S +  + +GSL  G  A P    + PL
Sbjct: 248 IGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVS-RGTDSTGSLVFGREALPVGASWVPL 307

Query: 267 LRNPRRSSLYYVNLIAVRVGRRVVDIPPSAFAFNPTTGSGTIIDSGTVFTRLVAPAYTAV 326
           +RNPR  S YYV L  + VG   + +P   F    T   G ++D+GT  TRL   AY A 
Sbjct: 308 VRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAF 367

Query: 327 RDEFRRRVGN-ATVSPLGGFDTCYT----VPIVSPTITFMFA-GMNVTLPPDNFLIHSTA 386
           RD F+ +  N    S +  FDTCY     V +  PT++F F  G  +TLP  NFL+    
Sbjct: 368 RDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPARNFLMPVDD 427

Query: 387 GSTTCLAMAAAPDNVNSVLNVIANMQQQNHRLLFDIPNSRLGVAREPC 426
             T C A AA+P      L++I N+QQ+  ++ FD  N  +G     C
Sbjct: 428 SGTYCFAFAASPTG----LSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

BLAST of MS004278 vs. ExPASy TrEMBL
Match: A0A6J1GYP2 (aspartyl protease AED3 OS=Cucurbita moschata OX=3662 GN=LOC111458686 PE=4 SV=1)

HSP 1 Score: 717.6 bits (1851), Expect = 3.0e-203
Identity = 357/407 (87.71%), Postives = 384/407 (94.35%), Query Frame = 0

Query: 24  AAADDSATLKVFHV----SSVLKSKPLSWAENVVQMLRKDQARLQFLSSLVARRSVAPIA 83
           AAAD S+TL+VFH+    S    SKPLSWA+NV+QM  KDQARLQFLSSLVAR+SVAPIA
Sbjct: 31  AAADRSSTLQVFHIFSPCSPFRPSKPLSWADNVLQMQAKDQARLQFLSSLVARKSVAPIA 90

Query: 84  SARQLIQSPTYVVRAKIGTPAQTLLMALDTSNDAAWIPCSGCLGCPSATVFSSDKSSSFK 143
           SARQLIQSPT+VVRAKIGTPAQTLLMALDTSNDAAWIPCSGC+GCPS TVFSSDKSSSF+
Sbjct: 91  SARQLIQSPTFVVRAKIGTPAQTLLMALDTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFR 150

Query: 144 PLPCNSPQCNQVPNPSCGGSACGFNLTYGSSTVAADLVQDNITLANDSVPAYTFGCITKA 203
           PLPC SPQCNQVPNPSC  SACGFNLTYGSSTVAADLVQDNITLANDSVPAYTFGCITKA
Sbjct: 151 PLPCRSPQCNQVPNPSCSSSACGFNLTYGSSTVAADLVQDNITLANDSVPAYTFGCITKA 210

Query: 204 TGASVPPQGLLGLGRGPLSLLSQTQNLYQSTFSYCLPSFKSPNFSGSLRLGPVAQPKRIK 263
           TG+SVPPQGLLGLGRGPLSLL Q+Q+LY+STFSYCLPSFKS  FSGSLRLGPVAQPKRIK
Sbjct: 211 TGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFSYCLPSFKSTGFSGSLRLGPVAQPKRIK 270

Query: 264 YTPLLRNPRRSSLYYVNLIAVRVGRRVVDIPPSAFAFNPTTGSGTIIDSGTVFTRLVAPA 323
           YTPLLRNPRRSSLYYVNLIA+RVGRR+VDIPPSA AFN  TG+GTIIDSGT FTRLVAPA
Sbjct: 271 YTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSALAFNSATGAGTIIDSGTTFTRLVAPA 330

Query: 324 YTAVRDEFRRRVGNATVSPLGGFDTCYTVPIVSPTITFMFAGMNVTLPPDNFLIHSTAGS 383
           YTAVR+EFRRR+GNAT+S LGGFDTCYTVPI+SPTITFMFAGMNVTLPPDNFL+HS+AG+
Sbjct: 331 YTAVRNEFRRRIGNATISSLGGFDTCYTVPIISPTITFMFAGMNVTLPPDNFLLHSSAGT 390

Query: 384 TTCLAMAAAPDNVNSVLNVIANMQQQNHRLLFDIPNSRLGVAREPCS 427
           TTCLAMAAAPDNVNSVLNVIA+MQQQNHR+LFD+PNSR+GVAREPCS
Sbjct: 391 TTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDVPNSRIGVAREPCS 437

BLAST of MS004278 vs. ExPASy TrEMBL
Match: A0A5A7SU62 (Aspartyl protease AED3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G003150 PE=4 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 6.8e-203
Identity = 368/442 (83.26%), Postives = 395/442 (89.37%), Query Frame = 0

Query: 2   NNPHSHFLPLTVVVVLLLHCAAAAA------------DDSATLKVFHV----SSVLKSKP 61
           NN  S FLPL ++++LLL  + + A            D S+TLKVFH+    S    SKP
Sbjct: 2   NNTKSPFLPLPLLLLLLLLFSISTAKSHIPLNCNPADDRSSTLKVFHIFSPCSPFRPSKP 61

Query: 62  LSWAENVVQMLRKDQARLQFLSSLVARRSVAPIASARQLIQSPTYVVRAKIGTPAQTLLM 121
           LSWA+NV+QM  KDQARLQFLSSLVARRSV PIASARQLIQSPT+VVRAKIGTPAQTLL+
Sbjct: 62  LSWADNVLQMQAKDQARLQFLSSLVARRSVVPIASARQLIQSPTFVVRAKIGTPAQTLLL 121

Query: 122 ALDTSNDAAWIPCSGCLGCPSATVFSSDKSSSFKPLPCNSPQCNQVPNPSCGGSACGFNL 181
           ALDTSNDAAWIPCSGC+GCPS TVFSSDKSSSF+PLPC SPQCNQVPNPSC G+ACGFNL
Sbjct: 122 ALDTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCQSPQCNQVPNPSCSGTACGFNL 181

Query: 182 TYGSSTVAADLVQDNITLANDSVPAYTFGCITKATGASVPPQGLLGLGRGPLSLLSQTQN 241
           TYGSSTVAADLVQDN+TLA DSVPAYTFGCI KATG+SVPPQGLLGLGRGPLSLL Q+Q+
Sbjct: 182 TYGSSTVAADLVQDNVTLATDSVPAYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQS 241

Query: 242 LYQSTFSYCLPSFKSPNFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAVRVGRR 301
           LY+STFSYCLPSFKS NFSGSLRLGPVAQP RIKYTPLLRNPRRSSLYYVNLIA+RVGRR
Sbjct: 242 LYRSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLIAIRVGRR 301

Query: 302 VVDIPPSAFAFNPTTGSGTIIDSGTVFTRLVAPAYTAVRDEFRRRVG-NATVSPLGGFDT 361
           +VDIPPSA AFN  TG+GTIIDSGT FTRLVAPAYTAVRDEFRRRVG N TVS LGGFDT
Sbjct: 302 IVDIPPSALAFNSATGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRNVTVSSLGGFDT 361

Query: 362 CYTVPIVSPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIANMQQ 421
           CYTVPI+SPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIA+MQQ
Sbjct: 362 CYTVPIISPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQ 421

Query: 422 QNHRLLFDIPNSRLGVAREPCS 427
           QNHR+LFDIPNSR+GVAREPCS
Sbjct: 422 QNHRILFDIPNSRVGVAREPCS 443

BLAST of MS004278 vs. ExPASy TrEMBL
Match: A0A1S3BGB2 (aspartyl protease AED3 OS=Cucumis melo OX=3656 GN=LOC103489519 PE=4 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 6.8e-203
Identity = 368/442 (83.26%), Postives = 395/442 (89.37%), Query Frame = 0

Query: 2   NNPHSHFLPLTVVVVLLLHCAAAAA------------DDSATLKVFHV----SSVLKSKP 61
           NN  S FLPL ++++LLL  + + A            D S+TLKVFH+    S    SKP
Sbjct: 2   NNTKSPFLPLPLLLLLLLLFSISTAKSHIPLNCNPADDRSSTLKVFHIFSPCSPFRPSKP 61

Query: 62  LSWAENVVQMLRKDQARLQFLSSLVARRSVAPIASARQLIQSPTYVVRAKIGTPAQTLLM 121
           LSWA+NV+QM  KDQARLQFLSSLVARRSV PIASARQLIQSPT+VVRAKIGTPAQTLL+
Sbjct: 62  LSWADNVLQMQAKDQARLQFLSSLVARRSVVPIASARQLIQSPTFVVRAKIGTPAQTLLL 121

Query: 122 ALDTSNDAAWIPCSGCLGCPSATVFSSDKSSSFKPLPCNSPQCNQVPNPSCGGSACGFNL 181
           ALDTSNDAAWIPCSGC+GCPS TVFSSDKSSSF+PLPC SPQCNQVPNPSC G+ACGFNL
Sbjct: 122 ALDTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCQSPQCNQVPNPSCSGTACGFNL 181

Query: 182 TYGSSTVAADLVQDNITLANDSVPAYTFGCITKATGASVPPQGLLGLGRGPLSLLSQTQN 241
           TYGSSTVAADLVQDN+TLA DSVPAYTFGCI KATG+SVPPQGLLGLGRGPLSLL Q+Q+
Sbjct: 182 TYGSSTVAADLVQDNVTLATDSVPAYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQS 241

Query: 242 LYQSTFSYCLPSFKSPNFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAVRVGRR 301
           LY+STFSYCLPSFKS NFSGSLRLGPVAQP RIKYTPLLRNPRRSSLYYVNLIA+RVGRR
Sbjct: 242 LYRSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLIAIRVGRR 301

Query: 302 VVDIPPSAFAFNPTTGSGTIIDSGTVFTRLVAPAYTAVRDEFRRRVG-NATVSPLGGFDT 361
           +VDIPPSA AFN  TG+GTIIDSGT FTRLVAPAYTAVRDEFRRRVG N TVS LGGFDT
Sbjct: 302 IVDIPPSALAFNSATGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRNVTVSSLGGFDT 361

Query: 362 CYTVPIVSPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIANMQQ 421
           CYTVPI+SPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIA+MQQ
Sbjct: 362 CYTVPIISPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQ 421

Query: 422 QNHRLLFDIPNSRLGVAREPCS 427
           QNHR+LFDIPNSR+GVAREPCS
Sbjct: 422 QNHRILFDIPNSRVGVAREPCS 443

BLAST of MS004278 vs. ExPASy TrEMBL
Match: A0A6J1JIM3 (aspartyl protease AED3-like OS=Cucurbita maxima OX=3661 GN=LOC111486144 PE=4 SV=1)

HSP 1 Score: 713.8 bits (1841), Expect = 4.4e-202
Identity = 355/407 (87.22%), Postives = 384/407 (94.35%), Query Frame = 0

Query: 24  AAADDSATLKVFHV----SSVLKSKPLSWAENVVQMLRKDQARLQFLSSLVARRSVAPIA 83
           AAAD S+TL+VFH+    S    SKPLSWA+NV+QM  KDQARLQFLSSLVA +SVAPIA
Sbjct: 33  AAADRSSTLQVFHIFSPCSPFRPSKPLSWADNVLQMQAKDQARLQFLSSLVACKSVAPIA 92

Query: 84  SARQLIQSPTYVVRAKIGTPAQTLLMALDTSNDAAWIPCSGCLGCPSATVFSSDKSSSFK 143
           SARQLIQSPT+VVRAKIGTPAQTLLMALDTSNDAAWIPCSGC+GCPS TVFSSDKS+SF+
Sbjct: 93  SARQLIQSPTFVVRAKIGTPAQTLLMALDTSNDAAWIPCSGCVGCPSTTVFSSDKSTSFR 152

Query: 144 PLPCNSPQCNQVPNPSCGGSACGFNLTYGSSTVAADLVQDNITLANDSVPAYTFGCITKA 203
           PLPC SPQCNQVPNPSC  SACGFNLTYGSSTVAADLVQDNITLANDSVPAYTFGCITKA
Sbjct: 153 PLPCRSPQCNQVPNPSCSSSACGFNLTYGSSTVAADLVQDNITLANDSVPAYTFGCITKA 212

Query: 204 TGASVPPQGLLGLGRGPLSLLSQTQNLYQSTFSYCLPSFKSPNFSGSLRLGPVAQPKRIK 263
           TG+SVPPQGLLGLGRGPLSLL Q+Q+LY+STFSYCLPSFKS +FSGSLRLGPVAQPKRIK
Sbjct: 213 TGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFSYCLPSFKSTSFSGSLRLGPVAQPKRIK 272

Query: 264 YTPLLRNPRRSSLYYVNLIAVRVGRRVVDIPPSAFAFNPTTGSGTIIDSGTVFTRLVAPA 323
           YTPLLRNPRRSSLYYVNLIA+RVGRR+VDIPPSA AFN  TG+GTIIDSGT FTRLVAPA
Sbjct: 273 YTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSALAFNSATGAGTIIDSGTTFTRLVAPA 332

Query: 324 YTAVRDEFRRRVGNATVSPLGGFDTCYTVPIVSPTITFMFAGMNVTLPPDNFLIHSTAGS 383
           YTAVR+EFRRR+GNAT+S LGGFDTCYTVPI+SPTITFMFAGMNVTLPPDNFL+HS+AG+
Sbjct: 333 YTAVRNEFRRRIGNATISSLGGFDTCYTVPIISPTITFMFAGMNVTLPPDNFLLHSSAGT 392

Query: 384 TTCLAMAAAPDNVNSVLNVIANMQQQNHRLLFDIPNSRLGVAREPCS 427
           TTCLAMAAAPDNVNSVLNVIA+MQQQNHR+LFD+PNSR+GVAREPCS
Sbjct: 393 TTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDVPNSRMGVAREPCS 439

BLAST of MS004278 vs. ExPASy TrEMBL
Match: A0A0A0KTR5 (Aspartic proteinase nepenthesin-1 OS=Cucumis sativus OX=3659 GN=Csa_5G623870 PE=4 SV=1)

HSP 1 Score: 703.4 bits (1814), Expect = 5.9e-199
Identity = 362/439 (82.46%), Postives = 389/439 (88.61%), Query Frame = 0

Query: 2   NNPHSHFLPLTVVVVLLLHCAA---------AAADDSATLKVFHV----SSVLKSKPLSW 61
           NN  S FL L +++ L     A          AAD S+TL+VFH+    S    SKPLSW
Sbjct: 2   NNTKSPFLLLLLLLHLFSISTAKSHIPSNCNPAADRSSTLQVFHIFSPCSPFRPSKPLSW 61

Query: 62  AENVVQMLRKDQARLQFLSSLVARRSVAPIASARQLIQSPTYVVRAKIGTPAQTLLMALD 121
           A+NV+QM  KDQARLQFLSSLVARRS  PIASARQLIQSPT+VVRAKIGTPAQTLL+ALD
Sbjct: 62  ADNVLQMQAKDQARLQFLSSLVARRSFVPIASARQLIQSPTFVVRAKIGTPAQTLLLALD 121

Query: 122 TSNDAAWIPCSGCLGCPSATVFSSDKSSSFKPLPCNSPQCNQVPNPSCGGSACGFNLTYG 181
           TSNDAAWIPCSGC+GCPS TVFSSDKSSSF+PLPC SPQCNQVPNPSC GSACGFNLTYG
Sbjct: 122 TSNDAAWIPCSGCIGCPSTTVFSSDKSSSFRPLPCQSPQCNQVPNPSCSGSACGFNLTYG 181

Query: 182 SSTVAADLVQDNITLANDSVPAYTFGCITKATGASVPPQGLLGLGRGPLSLLSQTQNLYQ 241
           SSTVAADLVQDN+TLA DSVP+YTFGCI KATG+SVPPQGLLGLGRGPLSLL Q+Q+LYQ
Sbjct: 182 SSTVAADLVQDNLTLATDSVPSYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQSLYQ 241

Query: 242 STFSYCLPSFKSPNFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAVRVGRRVVD 301
           STFSYCLPSFKS NFSGSLRLGPVAQP RIKYTPLLRNPRRSSLYYVNLI++RVGR++VD
Sbjct: 242 STFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLISIRVGRKIVD 301

Query: 302 IPPSAFAFNPTTGSGTIIDSGTVFTRLVAPAYTAVRDEFRRRVG-NATVSPLGGFDTCYT 361
           IPPSA AFN  TG+GT+IDSGT FTRLVAPAYTAVRDEFRRRVG N TVS LGGFDTCYT
Sbjct: 302 IPPSALAFNSATGAGTVIDSGTTFTRLVAPAYTAVRDEFRRRVGRNVTVSSLGGFDTCYT 361

Query: 362 VPIVSPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIANMQQQNH 421
           VPI+SPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIA+MQQQNH
Sbjct: 362 VPIISPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQNH 421

Query: 422 RLLFDIPNSRLGVAREPCS 427
           R+LFDIPNSR+GVARE CS
Sbjct: 422 RILFDIPNSRVGVARESCS 440

BLAST of MS004278 vs. TAIR 10
Match: AT5G07030.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 562.4 bits (1448), Expect = 3.1e-160
Identity = 275/408 (67.40%), Postives = 334/408 (81.86%), Query Frame = 0

Query: 27  DDSATLKVFHVSS----VLKSKPLSWAENVVQMLRKDQARLQFLSSLVARRSVAPIASAR 86
           D  +TL++FH+ S       S PLSW   V+Q L +DQARLQ+LSSLVA RSV PIAS R
Sbjct: 48  DQGSTLRIFHIDSPCSPFKSSSPLSWEARVLQTLAQDQARLQYLSSLVAGRSVVPIASGR 107

Query: 87  QLIQSPTYVVRAKIGTPAQTLLMALDTSNDAAWIPCSGCLGCPSATVFSSDKSSSFKPLP 146
           Q++QS TY+V+A IGTPAQ LL+A+DTS+D AWIPCSGC+GCPS T FS  KS+SFK + 
Sbjct: 108 QMLQSTTYIVKALIGTPAQPLLLAMDTSSDVAWIPCSGCVGCPSNTAFSPAKSTSFKNVS 167

Query: 147 CNSPQCNQVPNPSCGGSACGFNLTYGSSTVAADLVQDNITLANDSVPAYTFGCITKATGA 206
           C++PQC QVPNP+CG  AC FNLTYGSS++AA+L QD I LA D + A+TFGC+ K  G 
Sbjct: 168 CSAPQCKQVPNPTCGARACSFNLTYGSSSIAANLSQDTIRLAADPIKAFTFGCVNKVAGG 227

Query: 207 SV--PPQGLLGLGRGPLSLLSQTQNLYQSTFSYCLPSFKSPNFSGSLRLGPVAQPKRIKY 266
               PPQGLLGLGRGPLSL+SQ Q++Y+STFSYCLPSF+S  FSGSLRLGP +QP+R+KY
Sbjct: 228 GTIPPPQGLLGLGRGPLSLMSQAQSIYKSTFSYCLPSFRSLTFSGSLRLGPTSQPQRVKY 287

Query: 267 TPLLRNPRRSSLYYVNLIAVRVGRRVVDIPPSAFAFNPTTGSGTIIDSGTVFTRLVAPAY 326
           T LLRNPRRSSLYYVNL+A+RVGR+VVD+PP+A AFNP+TG+GTI DSGTV+TRL  P Y
Sbjct: 288 TQLLRNPRRSSLYYVNLVAIRVGRKVVDLPPAAIAFNPSTGAGTIFDSGTVYTRLAKPVY 347

Query: 327 TAVRDEFRRRV--GNATVSPLGGFDTCYTVPIVSPTITFMFAGMNVTLPPDNFLIHSTAG 386
            AVR+EFR+RV    A V+ LGGFDTCY+  +  PTITFMF G+N+T+P DN ++HSTAG
Sbjct: 348 EAVRNEFRKRVKPTTAVVTSLGGFDTCYSGQVKVPTITFMFKGVNMTMPADNLMLHSTAG 407

Query: 387 STTCLAMAAAPDNVNSVLNVIANMQQQNHRLLFDIPNSRLGVAREPCS 427
           ST+CLAMAAAP+NVNSV+NVIA+MQQQNHR+L D+PN RLG+ARE CS
Sbjct: 408 STSCLAMAAAPENVNSVVNVIASMQQQNHRVLIDVPNGRLGLARERCS 455

BLAST of MS004278 vs. TAIR 10
Match: AT3G54400.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 545.4 bits (1404), Expect = 4.0e-155
Identity = 277/429 (64.57%), Postives = 341/429 (79.49%), Query Frame = 0

Query: 6   SHFLPLTVVVVLL----LHCAAAAADDSATLKVFHVSSVLK--SKPLSWAENVVQMLRKD 65
           SH L L + +++L    ++C   +   S+ L+VFH++S+       +SWA+ ++Q    D
Sbjct: 3   SHLLILLISLLILKSESINCNEKS--HSSDLRVFHINSLCSPFKTSVSWADTLLQ----D 62

Query: 66  QARLQFLSSLV-ARRSVAPIASARQLIQSPTYVVRAKIGTPAQTLLMALDTSNDAAWIPC 125
           +AR  +LSSL   R+S  PIAS R ++QSPTY+VRA IGTPAQ +L+ALDTSNDAAWIPC
Sbjct: 63  KARFLYLSSLAGVRKSSVPIASGRAIVQSPTYIVRANIGTPAQPMLVALDTSNDAAWIPC 122

Query: 126 SGCLGCPSATVFSSDKSSSFKPLPCNSPQCNQVPNPSCGGS-ACGFNLTYGSSTVAADLV 185
           SGC+GC S+ +F   KSSS + L C +PQC Q PNPSC  S +CGFN+TYG ST+ A L 
Sbjct: 123 SGCVGCSSSVLFDPSKSSSSRTLQCEAPQCKQAPNPSCTVSKSCGFNMTYGGSTIEAYLT 182

Query: 186 QDNITLANDSVPAYTFGCITKATGASVPPQGLLGLGRGPLSLLSQTQNLYQSTFSYCLPS 245
           QD +TLA+D +P YTFGCI KA+G S+P QGL+GLGRGPLSL+SQ+QNLYQSTFSYCLP+
Sbjct: 183 QDTLTLASDVIPNYTFGCINKASGTSLPAQGLMGLGRGPLSLISQSQNLYQSTFSYCLPN 242

Query: 246 FKSPNFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAVRVGRRVVDIPPSAFAFN 305
            KS NFSGSLRLGP  QP RIK TPLL+NPRRSSLYYVNL+ +RVG ++VDIP SA AF+
Sbjct: 243 SKSSNFSGSLRLGPKNQPIRIKTTPLLKNPRRSSLYYVNLVGIRVGNKIVDIPTSALAFD 302

Query: 306 PTTGSGTIIDSGTVFTRLVAPAYTAVRDEFRRRVGNATVSPLGGFDTCYTVPIVSPTITF 365
           P TG+GTI DSGTV+TRLV PAY AVR+EFRRRV NA  + LGGFDTCY+  +V P++TF
Sbjct: 303 PATGAGTIFDSGTVYTRLVEPAYVAVRNEFRRRVKNANATSLGGFDTCYSGSVVFPSVTF 362

Query: 366 MFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIANMQQQNHRLLFDIPNSR 425
           MFAGMNVTLPPDN LIHS+AG+ +CLAMAAAP NVNSVLNVIA+MQQQNHR+L D+PNSR
Sbjct: 363 MFAGMNVTLPPDNLLIHSSAGNLSCLAMAAAPVNVNSVLNVIASMQQQNHRVLIDVPNSR 422

Query: 426 LGVAREPCS 427
           LG++RE C+
Sbjct: 423 LGISRETCT 425

BLAST of MS004278 vs. TAIR 10
Match: AT1G09750.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 404.8 bits (1039), Expect = 8.4e-113
Identity = 223/437 (51.03%), Postives = 289/437 (66.13%), Query Frame = 0

Query: 9   LPLTVVVVLLLHCAAAAADDSATLKVFHVSSVLKSKPL-------SWAENVVQMLRKDQA 68
           LP T        CA AA D S  L +  +++  K  P        S  + V+ M   D  
Sbjct: 16  LPFTFTTATRDTCATAAPDGSDDLSIIPINA--KCSPFAPTHVSASVIDTVLHMASSDSH 75

Query: 69  RLQFLSSLVA---RRSVAPIASARQLIQSPTYVVRAKIGTPAQTLLMALDTSNDAAWIPC 128
           RL +LSSLVA   + +  P+AS  QL     YVVRAK+GTP Q + M LDTSNDA W+PC
Sbjct: 76  RLTYLSSLVAGKPKPTSVPVASGNQL-HIGNYVVRAKLGTPPQLMFMVLDTSNDAVWLPC 135

Query: 129 SGCLGCPSA-TVFSSDKSSSFKPLPCNSPQCNQV-----PNPSCGGSACGFNLTY-GSST 188
           SGC GC +A T F+++ SS++  + C++ QC Q      P+ S   S C FN +Y G S+
Sbjct: 136 SGCSGCSNASTSFNTNSSSTYSTVSCSTAQCTQARGLTCPSSSPQPSVCSFNQSYGGDSS 195

Query: 189 VAADLVQDNITLANDSVPAYTFGCITKATGASVPPQGLLGLGRGPLSLLSQTQNLYQSTF 248
            +A LVQD +TLA D +P ++FGCI  A+G S+PPQGL+GLGRGP+SL+SQT +LY   F
Sbjct: 196 FSASLVQDTLTLAPDVIPNFSFGCINSASGNSLPPQGLMGLGRGPMSLVSQTTSLYSGVF 255

Query: 249 SYCLPSFKSPNFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAVRVGRRVVDIPP 308
           SYCLPSF+S  FSGSL+LG + QPK I+YTPLLRNPRR SLYYVNL  V VG   V + P
Sbjct: 256 SYCLPSFRSFYFSGSLKLGLLGQPKSIRYTPLLRNPRRPSLYYVNLTGVSVGSVQVPVDP 315

Query: 309 SAFAFNPTTGSGTIIDSGTVFTRLVAPAYTAVRDEFRRRVGNATVSPLGGFDTCYTV--P 368
               F+  +G+GTIIDSGTV TR   P Y A+RDEFR++V  ++ S LG FDTC++    
Sbjct: 316 VYLTFDANSGAGTIIDSGTVITRFAQPVYEAIRDEFRKQVNVSSFSTLGAFDTCFSADNE 375

Query: 369 IVSPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIANMQQQNHRL 427
            V+P IT     +++ LP +N LIHS+AG+ TCL+MA    N N+VLNVIAN+QQQN R+
Sbjct: 376 NVAPKITLHMTSLDLKLPMENTLIHSSAGTLTCLSMAGIRQNANAVLNVIANLQQQNLRI 435

BLAST of MS004278 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 199.5 bits (506), Expect = 5.4e-51
Identity = 137/426 (32.16%), Postives = 211/426 (49.53%), Query Frame = 0

Query: 26  ADDSATLKVFHVSSVLKSKPLSWAENVVQMLRKDQARLQFLSSLVAR---RSVA----PI 85
           +  S TL + H+ ++  +K     E     L++D  R++ +++L A+   R+V     P 
Sbjct: 68  SSSSITLNLDHIDALSSNKTPD--ELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAPRPG 127

Query: 86  ASARQLIQ-----SPTYVVRAKIGTPAQTLLMALDTSNDAAWIPCSGCLGCPSAT--VFS 145
             +  ++      S  Y  R  +GTPA+ + M LDT +D  W+ C+ C  C S +  +F 
Sbjct: 128 GFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFD 187

Query: 146 SDKSSSFKPLPCNSPQCNQVPNPSCG--GSACGFNLTYG-SSTVAADLVQDNITLANDSV 205
             KS ++  +PC+SP C ++ +  C      C + ++YG  S    D   + +T   + V
Sbjct: 188 PRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRV 247

Query: 206 PAYTFGCITKATGASVPPQGLLGLGRGPLSLLSQTQNLYQSTFSYCLPSFKSPNFSGSLR 265
                GC     G  V   GLLGLG+G LS   QT + +   FSYCL    + +   S+ 
Sbjct: 248 KGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVV 307

Query: 266 LGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAVRV-GRRVVDIPPSAFAFNPTTGSGTIID 325
            G  A  +  ++TPLL NP+  + YYV L+ + V G RV  +  S F  +     G IID
Sbjct: 308 FGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIID 367

Query: 326 SGTVFTRLVAPAYTAVRDEFRRRVGNATVS---PLGGFDTCYTV----PIVSPTITFMFA 385
           SGT  TRL+ PAY A+RD F  RVG  T+        FDTC+ +     +  PT+   F 
Sbjct: 368 SGTSVTRLIRPAYIAMRDAF--RVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFR 427

Query: 386 GMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIANMQQQNHRLLFDIPNSRLGV 427
           G +V+LP  N+LI        C A A         L++I N+QQQ  R+++D+ +SR+G 
Sbjct: 428 GADVSLPATNYLIPVDTNGKFCFAFAGTMGG----LSIIGNIQQQGFRVVYDLASSRVGF 485

BLAST of MS004278 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 196.4 bits (498), Expect = 4.5e-50
Identity = 146/430 (33.95%), Postives = 204/430 (47.44%), Query Frame = 0

Query: 29  SATLKVFHVSSVLKSKPLSWAENVVQMLRKDQARLQFLSSLVA--------RR------- 88
           S ++ + HV ++      S A+     L++D  R++ ++SL A        +R       
Sbjct: 60  SLSVHLSHVDALSSFSDASPADLFNLRLQRDSLRVKSITSLAAVSTGRNATKRTPRTAGG 119

Query: 89  -SVAPIASARQLIQSPTYVVRAKIGTPAQTLLMALDTSNDAAWIPCSGCLGCPSAT--VF 148
            S A I+   Q   S  Y +R  +GTPA  + M LDT +D  W+ CS C  C + T  +F
Sbjct: 120 FSGAVISGLSQ--GSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSPCKACYNQTDAIF 179

Query: 149 SSDKSSSFKPLPCNSPQCNQVPNPS-C---GGSACGFNLTYG-SSTVAADLVQDNITLAN 208
              KS +F  +PC S  C ++ + S C       C + ++YG  S    D   + +T   
Sbjct: 180 DPKKSKTFATVPCGSRLCRRLDDSSECVTRRSKTCLYQVSYGDGSFTEGDFSTETLTFHG 239

Query: 209 DSVPAYTFGCITKATGASVPPQGLLGLGRGPLSLLSQTQNLYQSTFSYCLPSFKSPNFS- 268
             V     GC     G  V   GLLGLGRG LS  SQT+N Y   FSYCL    S   S 
Sbjct: 240 ARVDHVPLGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTKNRYNGKFSYCLVDRTSSGSSS 299

Query: 269 ---GSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAVRV-GRRVVDIPPSAFAFNPTT 328
               ++  G  A PK   +TPLL NP+  + YY+ L+ + V G RV  +  S F  + T 
Sbjct: 300 KPPSTIVFGNAAVPKTSVFTPLLTNPKLDTFYYLQLLGISVGGSRVPGVSESQFKLDATG 359

Query: 329 GSGTIIDSGTVFTRLVAPAYTAVRDEFRRRVGNATVSP-LGGFDTCYTV----PIVSPTI 388
             G IIDSGT  TRL  PAY A+RD FR        +P    FDTC+ +     +  PT+
Sbjct: 360 NGGVIIDSGTSVTRLTQPAYVALRDAFRLGATKLKRAPSYSLFDTCFDLSGMTTVKVPTV 419

Query: 389 TFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIANMQQQNHRLLFDIPN 426
            F F G  V+LP  N+LI        C A A    +    L++I N+QQQ  R+ +D+  
Sbjct: 420 VFHFGGGEVSLPASNYLIPVNTEGRFCFAFAGTMGS----LSIIGNIQQQGFRVAYDLVG 479