MC05g1477 (gene) Bitter gourd (Dali-11) v1

BLAST of MC05g1477 vs. ExPASy Swiss-Prot
Match: Q9LZL3 (Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 4.1e-163
Identity = 291/419 (69.45%), Postives = 340/419 (81.15%), Query Frame = 0

Query: 37  SSLNPALVLPLKTQVIPPESVRRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELS 96
           SS +  LVLPLKT++ P +   R  DKL F HNV+LTV+LTVGTPPQN++MVIDTGSELS
Sbjct: 40  SSSSQTLVLPLKTRITPTD--HRPTDKLHFHHNVTLTVTLTVGTPPQNISMVIDTGSELS 99

Query: 97  WLHCNKSQNSSSNSSSTFNPIRSSSYSPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLS 156
           WL CN+S N   N  + F+P RSSSYSPIPCSS TC  +TRDF IPASCDS++LCHATLS
Sbjct: 100 WLRCNRSSN--PNPVNNFDPTRSSSYSPIPCSSPTCRTRTRDFLIPASCDSDKLCHATLS 159

Query: 157 YADASSSEGNLAADTFYIGNS-GISNVVFGCMDSIFSSNNEEDAKNTGLMGMNRGSLSFV 216
           YADASSSEGNLAA+ F+ GNS   SN++FGCM S+  S+ EED K TGL+GMNRGSLSF+
Sbjct: 160 YADASSSEGNLAAEIFHFGNSTNDSNLIFGCMGSVSGSDPEEDTKTTGLLGMNRGSLSFI 219

Query: 217 SQMGFPKFSYCIS-EYDFSGLLLLGDANYSWLGLAPLNYTPLIQMSTPLPYFDRVAYTVH 276
           SQMGFPKFSYCIS   DF G LLLGD+N++W  L PLNYTPLI++STPLPYFDRVAYTV 
Sbjct: 220 SQMGFPKFSYCISGTDDFPGFLLLGDSNFTW--LTPLNYTPLIRISTPLPYFDRVAYTVQ 279

Query: 277 LEGIKVSNKLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIR 336
           L GIKV+ KLLPIP+SV  PDHTGAGQTMVDSGTQFTFLLGP YTALR  FLN+T G + 
Sbjct: 280 LTGIKVNGKLLPIPKSVLVPDHTGAGQTMVDSGTQFTFLLGPVYTALRSHFLNRTNGILT 339

Query: 337 VLDDPNFVFQGAMDLCYR---VPLNQSRLPPLPAVTLVFRGAEMTVSGDRILYRVPGEIR 396
           V +DP+FVFQG MDLCYR   V +    L  LP V+LVF GAE+ VSG  +LYRVP    
Sbjct: 340 VYEDPDFVFQGTMDLCYRISPVRIRSGILHRLPTVSLVFEGAEIAVSGQPLLYRVPHLTV 399

Query: 397 GNDSIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLQKSRIGLAEIRCDLAGQKLGMG 451
           GNDS++CFTFGNSDL+G+EAYVIGH HQQN+W+EFDLQ+SRIGLA + CD++GQ+LG+G
Sbjct: 400 GNDSVYCFTFGNSDLMGMEAYVIGHHHQQNMWIEFDLQRSRIGLAPVECDVSGQRLGIG 452

BLAST of MC05g1477 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 4.9e-39
Identity = 120/372 (32.26%), Postives = 187/372 (50.27%), Query Frame = 0

Query: 74  VSLTVGTPPQNVTMVIDTGSELSWLHCNKSQNSSSNSSSTFNPIRSSSYSPIPCSSSTCT 133
           ++L++GTP Q  + ++DTGS+L W  C       + S+  FNP  SSS+S +PCSS  C 
Sbjct: 97  MNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQLC- 156

Query: 134 DQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADTFYIGNSGISNVVFGCMDSIFSS 193
            Q    P   +C SN  C  T  Y D S ++G++  +T   G+  I N+ FGC       
Sbjct: 157 -QALSSP---TC-SNNFCQYTYGYGDGSETQGSMGTETLTFGSVSIPNITFGC-----GE 216

Query: 194 NNE--EDAKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFS--GLLLLGDANYSWLGLA 253
           NN+        GL+GM RG LS  SQ+   KFSYC++    S    LLLG    S    +
Sbjct: 217 NNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGS 276

Query: 254 PLNYTPLIQMSTPLPYFDRVAYTVHLEGIKVSNKLLPIPESVFE-PDHTGAGQTMVDSGT 313
           P   T LIQ S+ +P F    Y + L G+ V +  LPI  S F    + G G  ++DSGT
Sbjct: 277 P--NTTLIQ-SSQIPTF----YYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGT 336

Query: 314 QFTFLLGPAYTALRDEFLNQTAGSIRVLDDPNFVFQGAMDLCYRVPLNQSRLPPLPAVTL 373
             T+ +  AY ++R EF++Q   ++ V++  +  F    DLC++ P + S L  +P   +
Sbjct: 337 TLTYFVNNAYQSVRQEFISQI--NLPVVNGSSSGF----DLCFQTPSDPSNL-QIPTFVM 396

Query: 374 VFRGAEMTVSGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDL 433
            F G ++ +  +         I  ++ + C   G+S   G+   + G++ QQN+ + +D 
Sbjct: 397 HFDGGDLELPSENYF------ISPSNGLICLAMGSSS-QGMS--IFGNIQQQNMLVVYDT 434

Query: 434 QKSRIGLAEIRC 441
             S +  A  +C
Sbjct: 457 GNSVVSFASAQC 434

BLAST of MC05g1477 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 4.1e-38
Identity = 110/369 (29.81%), Postives = 175/369 (47.43%), Query Frame = 0

Query: 74  VSLTVGTPPQNVTMVIDTGSELSWLHCNKSQNSSSNSSSTFNPIRSSSYSPIPCSSSTCT 133
           +++ +GTP  + + ++DTGS+L W  C       S  +  FNP  SSS+S +PC S  C 
Sbjct: 98  MNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQ 157

Query: 134 DQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADTFYIGNSGISNVVFGCMDSIFSS 193
           D      +P+   +N  C  T  Y D S+++G +A +TF    S + N+ FGC +    +
Sbjct: 158 D------LPSETCNNNECQYTYGYGDGSTTQGYMATETFTFETSSVPNIAFGCGE---DN 217

Query: 194 NNEEDAKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFS--GLLLLGDANYSWLGLAPL 253
                    GL+GM  G LS  SQ+G  +FSYC++ Y  S    L LG A       +P 
Sbjct: 218 QGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTLALGSAASGVPEGSP- 277

Query: 254 NYTPLIQMSTPLPYFDRVAYTVHLEGIKVSNKLLPIPESVFEPDHTGAGQTMVDSGTQFT 313
             T LI  S    Y     Y + L+GI V    L IP S F+    G G  ++DSGT  T
Sbjct: 278 -STTLIHSSLNPTY-----YYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLT 337

Query: 314 FLLGPAYTALRDEFLNQTAGSIRVLDDPNFVFQGAMDLCYRVPLNQSRLPPLPAVTLVFR 373
           +L   AY A+   F +Q   ++  +D+ +      +  C++ P + S +  +P +++ F 
Sbjct: 338 YLPQDAYNAVAQAFTDQI--NLPTVDESS----SGLSTCFQQPSDGSTV-QVPEISMQFD 397

Query: 374 GAEMTVSGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLQKS 433
           G  + +    IL      I   + + C   G+S  LG+   + G++ QQ   + +DLQ  
Sbjct: 398 GGVLNLGEQNIL------ISPAEGVICLAMGSSSQLGIS--IFGNIQQQETQVLYDLQNL 435

Query: 434 RIGLAEIRC 441
            +     +C
Sbjct: 458 AVSFVPTQC 435

BLAST of MC05g1477 vs. ExPASy Swiss-Prot
Match: O04496 (Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 7.5e-32
Identity = 122/382 (31.94%), Postives = 180/382 (47.12%), Query Frame = 0

Query: 68  HNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNKSQNSSSNSSSTFNPIRSSSYSPIPC 127
           H  +  V   +GTPPQ + MV+DT ++  WL C+   +  SN+S++FN   SS+YS + C
Sbjct: 100 HIGNYVVRAKLGTPPQLMFMVLDTSNDAVWLPCS-GCSGCSNASTSFNTNSSSTYSTVSC 159

Query: 128 SSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADTFYIGNSGISNVVFGCM 187
           S++ CT Q R    P+S     +C    SY   SS   +L  DT  +    I N  FGC+
Sbjct: 160 STAQCT-QARGLTCPSSSPQPSVCSFNQSYGGDSSFSASLVQDTLTLAPDVIPNFSFGCI 219

Query: 188 DSIFSSNNEEDAKNTGLMGMNRGSLSFVSQ---MGFPKFSYCISEYD---FSGLLLLGDA 247
           +S  +S N    +  GLMG+ RG +S VSQ   +    FSYC+  +    FSG L LG  
Sbjct: 220 NS--ASGNSLPPQ--GLMGLGRGPMSLVSQTTSLYSGVFSYCLPSFRSFYFSGSLKLG-- 279

Query: 248 NYSWLGL-APLNYTPLIQMSTPLPYFDRVAYTVHLEGIKVSNKLLPIPESVFEPDHTGAG 307
               LG    + YTPL++ +   P      Y V+L G+ V +  +P+       D     
Sbjct: 280 ---LLGQPKSIRYTPLLR-NPRRPSL----YYVNLTGVSVGSVQVPVDPVYLTFDANSGA 339

Query: 308 QTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVLDDPNFVFQGAMDLCYRVPLNQSRL 367
            T++DSGT  T    P Y A+RDEF  Q   S       +F   GA D C+    N++  
Sbjct: 340 GTIIDSGTVITRFAQPVYEAIRDEFRKQVNVS-------SFSTLGAFDTCFSAD-NEN-- 399

Query: 368 PPLPAVTLVFRGAEMTVSGDRILYRVPGEIRGNDSIHCFTF-GNSDLLGVEAYVIGHLHQ 427
              P +TL     ++ +  +  L           ++ C +  G          VI +L Q
Sbjct: 400 -VAPKITLHMTSLDLKLPMENTLIH-----SSAGTLTCLSMAGIRQNANAVLNVIANLQQ 449

Query: 428 QNVWMEFDLQKSRIGLAEIRCD 442
           QN+ + FD+  SRIG+A   C+
Sbjct: 460 QNLRILFDVPNSRIGIAPEPCN 449

BLAST of MC05g1477 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 2.9e-31
Identity = 115/368 (31.25%), Postives = 172/368 (46.74%), Query Frame = 0

Query: 78  VGTPPQNVTMVIDTGSELSWLHCNKSQNSSSNSSSTFNPIRSSSYSPIPCSSSTCTDQTR 137
           VGTP + + +V+DTGS+++W+ C    +    S   FNP  SS+Y  + CS+  C+    
Sbjct: 168 VGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCS---- 227

Query: 138 DFPIPASCDSNQLCHATLSYADASSSEGNLAADTFYIGNSG-ISNVVFGCMDSIFSSNNE 197
                ++C SN+ C   +SY D S + G LA DT   GNSG I+NV  GC       N  
Sbjct: 228 -LLETSACRSNK-CLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALGC----GHDNEG 287

Query: 198 EDAKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANYSWLGLAPLNYTPL 257
                 GL+G+  G LS  +QM    FSYC+ + D SG     D N   LG       PL
Sbjct: 288 LFTGAAGLLGLGGGVLSITNQMKATSFSYCLVDRD-SGKSSSLDFNSVQLGGGDAT-APL 347

Query: 258 IQMSTPLPYFDRVAYTVHLEGIKVSNKLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGP 317
           ++ +  +  F    Y V L G  V  + + +P+++F+ D +G+G  ++D GT  T L   
Sbjct: 348 LR-NKKIDTF----YYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQ 407

Query: 318 AYTALRDEFLNQTA----GSIRVLDDPNFVFQGAMDLCYRVPLNQSRLPPLPAVTLVFRG 377
           AY +LRD FL  T     GS  +            D CY    +      +P V   F G
Sbjct: 408 AYNSLRDAFLKLTVNLKKGSSSI---------SLFDTCY--DFSSLSTVKVPTVAFHFTG 467

Query: 378 AEMTVSGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLQKSR 437
            + ++      Y +P +  G     CF F  +        +IG++ QQ   + +DL K+ 
Sbjct: 468 GK-SLDLPAKNYLIPVDDSGT---FCFAFAPT---SSSLSIIGNVQQQGTRITYDLSKNV 500

Query: 438 IGLAEIRC 441
           IGL+  +C
Sbjct: 528 IGLSGNKC 500

BLAST of MC05g1477 vs. NCBI nr
Match: XP_022145548.1 (aspartic proteinase PCS1 [Momordica charantia])

HSP 1 Score: 909 bits (2350), Expect = 0.0
Identity = 451/451 (100.00%), Postives = 451/451 (100.00%), Query Frame = 0

Query: 1   MRDHIAFNFSNSPFLKSCFFFFLCTLICGFQNTKLCSSLNPALVLPLKTQVIPPESVRRS 60
           MRDHIAFNFSNSPFLKSCFFFFLCTLICGFQNTKLCSSLNPALVLPLKTQVIPPESVRRS
Sbjct: 1   MRDHIAFNFSNSPFLKSCFFFFLCTLICGFQNTKLCSSLNPALVLPLKTQVIPPESVRRS 60

Query: 61  PDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNKSQNSSSNSSSTFNPIRSS 120
           PDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNKSQNSSSNSSSTFNPIRSS
Sbjct: 61  PDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNKSQNSSSNSSSTFNPIRSS 120

Query: 121 SYSPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADTFYIGNSGIS 180
           SYSPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADTFYIGNSGIS
Sbjct: 121 SYSPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADTFYIGNSGIS 180

Query: 181 NVVFGCMDSIFSSNNEEDAKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGD 240
           NVVFGCMDSIFSSNNEEDAKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGD
Sbjct: 181 NVVFGCMDSIFSSNNEEDAKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGD 240

Query: 241 ANYSWLGLAPLNYTPLIQMSTPLPYFDRVAYTVHLEGIKVSNKLLPIPESVFEPDHTGAG 300
           ANYSWLGLAPLNYTPLIQMSTPLPYFDRVAYTVHLEGIKVSNKLLPIPESVFEPDHTGAG
Sbjct: 241 ANYSWLGLAPLNYTPLIQMSTPLPYFDRVAYTVHLEGIKVSNKLLPIPESVFEPDHTGAG 300

Query: 301 QTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVLDDPNFVFQGAMDLCYRVPLNQSRL 360
           QTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVLDDPNFVFQGAMDLCYRVPLNQSRL
Sbjct: 301 QTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVLDDPNFVFQGAMDLCYRVPLNQSRL 360

Query: 361 PPLPAVTLVFRGAEMTVSGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQQ 420
           PPLPAVTLVFRGAEMTVSGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQQ
Sbjct: 361 PPLPAVTLVFRGAEMTVSGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQQ 420

Query: 421 NVWMEFDLQKSRIGLAEIRCDLAGQKLGMGL 451
           NVWMEFDLQKSRIGLAEIRCDLAGQKLGMGL
Sbjct: 421 NVWMEFDLQKSRIGLAEIRCDLAGQKLGMGL 451

BLAST of MC05g1477 vs. NCBI nr
Match: XP_038905417.1 (aspartic proteinase PCS1 [Benincasa hispida])

HSP 1 Score: 827 bits (2135), Expect = 6.17e-301
Identity = 412/452 (91.15%), Postives = 432/452 (95.58%), Query Frame = 0

Query: 1   MRDH-IAFNFSNSPFLKSCFFFFLCTLICGFQNTKLCSSLNPALVLPLKTQVIPPESVRR 60
           MRD+  AFN S++ FLK CF FF CTL C FQN+KLCSSLNPALVLPLKTQVIPPESVRR
Sbjct: 1   MRDYCFAFNSSSNKFLKYCFIFFFCTLFCVFQNSKLCSSLNPALVLPLKTQVIPPESVRR 60

Query: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNKSQNSSSNSSSTFNPIRS 120
           SPDKLPFRHN+SLTVSLTVGTPPQNVTMVIDTGSELSWLHCN+SQNSSS SSSTFNP+RS
Sbjct: 61  SPDKLPFRHNISLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSS-SSSTFNPVRS 120

Query: 121 SSYSPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADTFYIGNSGI 180
           SSY+PIPCSSSTCTDQTRDFPI  SCDSNQ CHATLSYADASSSEGNLA DTFYIGNSGI
Sbjct: 121 SSYTPIPCSSSTCTDQTRDFPIRPSCDSNQFCHATLSYADASSSEGNLATDTFYIGNSGI 180

Query: 181 SNVVFGCMDSIFSSNNEEDAKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240
            NVVFGCMDSIFSSNNEED+KNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG
Sbjct: 181 PNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240

Query: 241 DANYSWLGLAPLNYTPLIQMSTPLPYFDRVAYTVHLEGIKVSNKLLPIPESVFEPDHTGA 300
           DAN+SWL  APLNYTPLI+MSTPLPYFDRVAYTV LEGIKVS+KLLPIPESVFEPDHTGA
Sbjct: 241 DANFSWL--APLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGA 300

Query: 301 GQTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVLDDPNFVFQGAMDLCYRVPLNQSR 360
           GQTMVDSGTQFTFLLGPAY ALRDEF+NQTAGSIRVL+DPNFVFQGAMDLCYRVP+NQ+R
Sbjct: 301 GQTMVDSGTQFTFLLGPAYAALRDEFVNQTAGSIRVLEDPNFVFQGAMDLCYRVPINQTR 360

Query: 361 LPPLPAVTLVFRGAEMTVSGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQ 420
           LPPLP+VTLVFRGAEMTV+GDRILYRVPGE RGNDSIHCFTFGNSDLLGVEA+VIGHLHQ
Sbjct: 361 LPPLPSVTLVFRGAEMTVTGDRILYRVPGERRGNDSIHCFTFGNSDLLGVEAFVIGHLHQ 420

Query: 421 QNVWMEFDLQKSRIGLAEIRCDLAGQKLGMGL 451
           QNVWMEFDL+KSRIGLAEIRCDLAGQKLGMGL
Sbjct: 421 QNVWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 449

BLAST of MC05g1477 vs. NCBI nr
Match: XP_023528618.1 (aspartic proteinase PCS1 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 818 bits (2114), Expect = 1.01e-297
Identity = 410/452 (90.71%), Postives = 429/452 (94.91%), Query Frame = 0

Query: 1   MRDH-IAFNFSNSPFLKSCFFFFLCTLICGFQNTKLCSSLNPALVLPLKTQVIPPESVRR 60
           MRD+ IAFN SN  FLKS F FFLCTL   FQN  LCSS NPAL+LPLKTQVIPPES+RR
Sbjct: 1   MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR 60

Query: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNKSQNSSSNSSSTFNPIRS 120
           SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCN+SQNSSS+SSSTFNP  S
Sbjct: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS 120

Query: 121 SSYSPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADTFYIGNSGI 180
           SSY+PIPCSSSTCTDQTRDFPIPASCDSN LCHATLSYADASSSEG LA DTFYIGNSGI
Sbjct: 121 SSYTPIPCSSSTCTDQTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 180

Query: 181 SNVVFGCMDSIFSSNNEEDAKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240
           SNVVFGCMDSIFSSNNEED+KNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG
Sbjct: 181 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240

Query: 241 DANYSWLGLAPLNYTPLIQMSTPLPYFDRVAYTVHLEGIKVSNKLLPIPESVFEPDHTGA 300
           DAN+SWL  APLNYTPLI+M+TPLPYFDRVAYTV LEGIKVS+KLLPIPESVFEPDHTGA
Sbjct: 241 DANFSWL--APLNYTPLIEMTTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGA 300

Query: 301 GQTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVLDDPNFVFQGAMDLCYRVPLNQSR 360
           GQTMVDSGTQFTFLLGPAYTALRDEFLN+TAGS RV +D NFVFQGAMDLCYRVP+NQ+R
Sbjct: 301 GQTMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTR 360

Query: 361 LPPLPAVTLVFRGAEMTVSGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQ 420
           LPPLP+VTLVFRGAEMTV+GDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEA+VIGHLHQ
Sbjct: 361 LPPLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQ 420

Query: 421 QNVWMEFDLQKSRIGLAEIRCDLAGQKLGMGL 451
           QNVWMEFDL+KSRIGLAEIRCDLAGQKLGMGL
Sbjct: 421 QNVWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 450

BLAST of MC05g1477 vs. NCBI nr
Match: XP_022935018.1 (aspartic proteinase PCS1 [Cucurbita moschata] >KAG6580532.1 Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 818 bits (2113), Expect = 1.44e-297
Identity = 410/452 (90.71%), Postives = 429/452 (94.91%), Query Frame = 0

Query: 1   MRDH-IAFNFSNSPFLKSCFFFFLCTLICGFQNTKLCSSLNPALVLPLKTQVIPPESVRR 60
           MRD+ IAFN SN  FLKS F FFLCTL   FQN  LCSS NPAL+LPLKTQVIPPES+RR
Sbjct: 1   MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR 60

Query: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNKSQNSSSNSSSTFNPIRS 120
           SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCN+SQNSSS+SSSTFNP  S
Sbjct: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS 120

Query: 121 SSYSPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADTFYIGNSGI 180
           SSY+PIPCSSSTCTD+TRDFPIPASCDSN LCHATLSYADASSSEG LA DTFYIGNSGI
Sbjct: 121 SSYTPIPCSSSTCTDRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 180

Query: 181 SNVVFGCMDSIFSSNNEEDAKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240
           SNVVFGCMDSIFSSNNEED+KNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG
Sbjct: 181 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240

Query: 241 DANYSWLGLAPLNYTPLIQMSTPLPYFDRVAYTVHLEGIKVSNKLLPIPESVFEPDHTGA 300
           DAN+SWL  APLNYTPLI+MSTPLPYFDRVAYTV LEGIKVS+KLLPIPESVFEPDHTGA
Sbjct: 241 DANFSWL--APLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGA 300

Query: 301 GQTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVLDDPNFVFQGAMDLCYRVPLNQSR 360
           GQTMVDSGTQFTFLLGPAYTALRDEFLN+TAGS RV +D NFVFQGAMDLCYRVP+NQ+R
Sbjct: 301 GQTMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTR 360

Query: 361 LPPLPAVTLVFRGAEMTVSGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQ 420
           LPPLP+VTLVFRGAEMTV+GDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEA+VIGHLHQ
Sbjct: 361 LPPLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQ 420

Query: 421 QNVWMEFDLQKSRIGLAEIRCDLAGQKLGMGL 451
           QNVWMEFDL+KSRIGLAEIRCDLAGQKLGMGL
Sbjct: 421 QNVWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 450

BLAST of MC05g1477 vs. NCBI nr
Match: XP_023528617.1 (aspartic proteinase PCS1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 818 bits (2114), Expect = 7.72e-297
Identity = 410/452 (90.71%), Postives = 429/452 (94.91%), Query Frame = 0

Query: 1   MRDH-IAFNFSNSPFLKSCFFFFLCTLICGFQNTKLCSSLNPALVLPLKTQVIPPESVRR 60
           MRD+ IAFN SN  FLKS F FFLCTL   FQN  LCSS NPAL+LPLKTQVIPPES+RR
Sbjct: 55  MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR 114

Query: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNKSQNSSSNSSSTFNPIRS 120
           SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCN+SQNSSS+SSSTFNP  S
Sbjct: 115 SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS 174

Query: 121 SSYSPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADTFYIGNSGI 180
           SSY+PIPCSSSTCTDQTRDFPIPASCDSN LCHATLSYADASSSEG LA DTFYIGNSGI
Sbjct: 175 SSYTPIPCSSSTCTDQTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 234

Query: 181 SNVVFGCMDSIFSSNNEEDAKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240
           SNVVFGCMDSIFSSNNEED+KNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG
Sbjct: 235 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 294

Query: 241 DANYSWLGLAPLNYTPLIQMSTPLPYFDRVAYTVHLEGIKVSNKLLPIPESVFEPDHTGA 300
           DAN+SWL  APLNYTPLI+M+TPLPYFDRVAYTV LEGIKVS+KLLPIPESVFEPDHTGA
Sbjct: 295 DANFSWL--APLNYTPLIEMTTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGA 354

Query: 301 GQTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVLDDPNFVFQGAMDLCYRVPLNQSR 360
           GQTMVDSGTQFTFLLGPAYTALRDEFLN+TAGS RV +D NFVFQGAMDLCYRVP+NQ+R
Sbjct: 355 GQTMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTR 414

Query: 361 LPPLPAVTLVFRGAEMTVSGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQ 420
           LPPLP+VTLVFRGAEMTV+GDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEA+VIGHLHQ
Sbjct: 415 LPPLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQ 474

Query: 421 QNVWMEFDLQKSRIGLAEIRCDLAGQKLGMGL 451
           QNVWMEFDL+KSRIGLAEIRCDLAGQKLGMGL
Sbjct: 475 QNVWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 504

BLAST of MC05g1477 vs. ExPASy TrEMBL
Match: A0A6J1CW88 (aspartic proteinase PCS1 OS=Momordica charantia OX=3673 GN=LOC111014974 PE=3 SV=1)

HSP 1 Score: 909 bits (2350), Expect = 0.0
Identity = 451/451 (100.00%), Postives = 451/451 (100.00%), Query Frame = 0

Query: 1   MRDHIAFNFSNSPFLKSCFFFFLCTLICGFQNTKLCSSLNPALVLPLKTQVIPPESVRRS 60
           MRDHIAFNFSNSPFLKSCFFFFLCTLICGFQNTKLCSSLNPALVLPLKTQVIPPESVRRS
Sbjct: 1   MRDHIAFNFSNSPFLKSCFFFFLCTLICGFQNTKLCSSLNPALVLPLKTQVIPPESVRRS 60

Query: 61  PDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNKSQNSSSNSSSTFNPIRSS 120
           PDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNKSQNSSSNSSSTFNPIRSS
Sbjct: 61  PDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNKSQNSSSNSSSTFNPIRSS 120

Query: 121 SYSPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADTFYIGNSGIS 180
           SYSPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADTFYIGNSGIS
Sbjct: 121 SYSPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADTFYIGNSGIS 180

Query: 181 NVVFGCMDSIFSSNNEEDAKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGD 240
           NVVFGCMDSIFSSNNEEDAKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGD
Sbjct: 181 NVVFGCMDSIFSSNNEEDAKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGD 240

Query: 241 ANYSWLGLAPLNYTPLIQMSTPLPYFDRVAYTVHLEGIKVSNKLLPIPESVFEPDHTGAG 300
           ANYSWLGLAPLNYTPLIQMSTPLPYFDRVAYTVHLEGIKVSNKLLPIPESVFEPDHTGAG
Sbjct: 241 ANYSWLGLAPLNYTPLIQMSTPLPYFDRVAYTVHLEGIKVSNKLLPIPESVFEPDHTGAG 300

Query: 301 QTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVLDDPNFVFQGAMDLCYRVPLNQSRL 360
           QTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVLDDPNFVFQGAMDLCYRVPLNQSRL
Sbjct: 301 QTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVLDDPNFVFQGAMDLCYRVPLNQSRL 360

Query: 361 PPLPAVTLVFRGAEMTVSGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQQ 420
           PPLPAVTLVFRGAEMTVSGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQQ
Sbjct: 361 PPLPAVTLVFRGAEMTVSGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQQ 420

Query: 421 NVWMEFDLQKSRIGLAEIRCDLAGQKLGMGL 451
           NVWMEFDLQKSRIGLAEIRCDLAGQKLGMGL
Sbjct: 421 NVWMEFDLQKSRIGLAEIRCDLAGQKLGMGL 451

BLAST of MC05g1477 vs. ExPASy TrEMBL
Match: A0A6J1F9C1 (aspartic proteinase PCS1 OS=Cucurbita moschata OX=3662 GN=LOC111442007 PE=3 SV=1)

HSP 1 Score: 818 bits (2113), Expect = 6.97e-298
Identity = 410/452 (90.71%), Postives = 429/452 (94.91%), Query Frame = 0

Query: 1   MRDH-IAFNFSNSPFLKSCFFFFLCTLICGFQNTKLCSSLNPALVLPLKTQVIPPESVRR 60
           MRD+ IAFN SN  FLKS F FFLCTL   FQN  LCSS NPAL+LPLKTQVIPPES+RR
Sbjct: 1   MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR 60

Query: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNKSQNSSSNSSSTFNPIRS 120
           SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCN+SQNSSS+SSSTFNP  S
Sbjct: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS 120

Query: 121 SSYSPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADTFYIGNSGI 180
           SSY+PIPCSSSTCTD+TRDFPIPASCDSN LCHATLSYADASSSEG LA DTFYIGNSGI
Sbjct: 121 SSYTPIPCSSSTCTDRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 180

Query: 181 SNVVFGCMDSIFSSNNEEDAKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240
           SNVVFGCMDSIFSSNNEED+KNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG
Sbjct: 181 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240

Query: 241 DANYSWLGLAPLNYTPLIQMSTPLPYFDRVAYTVHLEGIKVSNKLLPIPESVFEPDHTGA 300
           DAN+SWL  APLNYTPLI+MSTPLPYFDRVAYTV LEGIKVS+KLLPIPESVFEPDHTGA
Sbjct: 241 DANFSWL--APLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGA 300

Query: 301 GQTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVLDDPNFVFQGAMDLCYRVPLNQSR 360
           GQTMVDSGTQFTFLLGPAYTALRDEFLN+TAGS RV +D NFVFQGAMDLCYRVP+NQ+R
Sbjct: 301 GQTMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTR 360

Query: 361 LPPLPAVTLVFRGAEMTVSGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQ 420
           LPPLP+VTLVFRGAEMTV+GDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEA+VIGHLHQ
Sbjct: 361 LPPLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQ 420

Query: 421 QNVWMEFDLQKSRIGLAEIRCDLAGQKLGMGL 451
           QNVWMEFDL+KSRIGLAEIRCDLAGQKLGMGL
Sbjct: 421 QNVWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 450

BLAST of MC05g1477 vs. ExPASy TrEMBL
Match: A0A6J1J2F5 (aspartic proteinase PCS1 OS=Cucurbita maxima OX=3661 GN=LOC111482077 PE=3 SV=1)

HSP 1 Score: 816 bits (2108), Expect = 3.88e-297
Identity = 412/452 (91.15%), Postives = 429/452 (94.91%), Query Frame = 0

Query: 1   MRDH-IAFNFSNSPFLKSCFFFFLCTLICGFQNTKLCSSLNPALVLPLKTQVIPPESVRR 60
           MRD+ IAFN SN  FLKS F FFLC L   FQN  LCSSLNPAL+LPLKTQVIPPES+RR
Sbjct: 1   MRDYCIAFNSSNHKFLKSFFPFFLCILFSVFQNLILCSSLNPALLLPLKTQVIPPESIRR 60

Query: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNKSQNSSSNSSSTFNPIRS 120
           SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCN+SQNSSS SSSTFNP  S
Sbjct: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSS-SSSTFNPAGS 120

Query: 121 SSYSPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADTFYIGNSGI 180
           SSY+PIPCSSSTCTDQTRDFPIPASCDSN LCHATLSYADASSSEG LA DTFYIGNSGI
Sbjct: 121 SSYTPIPCSSSTCTDQTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 180

Query: 181 SNVVFGCMDSIFSSNNEEDAKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240
           SNVVFGCMDSIFSSNNEED+KNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG
Sbjct: 181 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240

Query: 241 DANYSWLGLAPLNYTPLIQMSTPLPYFDRVAYTVHLEGIKVSNKLLPIPESVFEPDHTGA 300
           DAN+SWL  APLNYTPLI+MSTPLPYFDRVAYTV LEGIKVS+KLLPIPESVFEPDHTGA
Sbjct: 241 DANFSWL--APLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGA 300

Query: 301 GQTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVLDDPNFVFQGAMDLCYRVPLNQSR 360
           GQTMVDSGTQFTFLLGPAYTALRDEFLN+TAGSIRV +D NFVFQGAMDLCYRVP+NQ+R
Sbjct: 301 GQTMVDSGTQFTFLLGPAYTALRDEFLNRTAGSIRVFEDSNFVFQGAMDLCYRVPMNQTR 360

Query: 361 LPPLPAVTLVFRGAEMTVSGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQ 420
           LPPLP+VTLVFRGAEMTV+GDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEA+VIGHLHQ
Sbjct: 361 LPPLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQ 420

Query: 421 QNVWMEFDLQKSRIGLAEIRCDLAGQKLGMGL 451
           QNVWMEFDL+KSRIGLAEIRCDLAGQKLGMGL
Sbjct: 421 QNVWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 449

BLAST of MC05g1477 vs. ExPASy TrEMBL
Match: A0A5D3DN23 (Aspartic proteinase PCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001570 PE=3 SV=1)

HSP 1 Score: 807 bits (2084), Expect = 1.90e-293
Identity = 404/453 (89.18%), Postives = 425/453 (93.82%), Query Frame = 0

Query: 1   MRDH-IAFNFSNSPFLKSCFFFFLCTLICGFQNTKLCSSLNPALVLPLKTQVIPPESVRR 60
           MR + +AF+ +N  FLKSCF FF C L   F N  LCSS+NPALVLPLKTQVIPPESVRR
Sbjct: 1   MRGYCLAFDSANIKFLKSCFLFFFCILFSVFYNIDLCSSVNPALVLPLKTQVIPPESVRR 60

Query: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNKSQNSSSNSSS-TFNPIR 120
           SPDKLPFRHN+SLTVSLTVGTPPQNVTMVIDTGSELSWLHCN SQNSSS+SSS TFNP+R
Sbjct: 61  SPDKLPFRHNISLTVSLTVGTPPQNVTMVIDTGSELSWLHCNTSQNSSSSSSSSTFNPVR 120

Query: 121 SSSYSPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADTFYIGNSG 180
           SSSYSPIPCSSSTCTDQTRDFPI  SCDSNQ CHATLSYADASSSEGNLA DTFYIGNSG
Sbjct: 121 SSSYSPIPCSSSTCTDQTRDFPIRPSCDSNQFCHATLSYADASSSEGNLATDTFYIGNSG 180

Query: 181 ISNVVFGCMDSIFSSNNEEDAKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLL 240
           I NVVFGCMDSIFSSNNEED+KNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLL
Sbjct: 181 IPNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLL 240

Query: 241 GDANYSWLGLAPLNYTPLIQMSTPLPYFDRVAYTVHLEGIKVSNKLLPIPESVFEPDHTG 300
           GDAN+SWL  APLNYTPLI+MSTPLPYFDRVAYTV LEGIKV++KLLPIPESVFEPDHTG
Sbjct: 241 GDANFSWL--APLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVAHKLLPIPESVFEPDHTG 300

Query: 301 AGQTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVLDDPNFVFQGAMDLCYRVPLNQS 360
           AGQTMVDSGTQFTFLLGPAYTALRD FLNQTAGS+R+ +DPNFVFQGAMDLCYRVP NQ+
Sbjct: 301 AGQTMVDSGTQFTFLLGPAYTALRDHFLNQTAGSLRLYEDPNFVFQGAMDLCYRVPTNQT 360

Query: 361 RLPPLPAVTLVFRGAEMTVSGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLH 420
           RLPPLP+VTLVFRGAEMTV+GDRILYRVPGE RGNDSIHCFTFGNSDLLGVEA+VIGHLH
Sbjct: 361 RLPPLPSVTLVFRGAEMTVTGDRILYRVPGERRGNDSIHCFTFGNSDLLGVEAFVIGHLH 420

Query: 421 QQNVWMEFDLQKSRIGLAEIRCDLAGQKLGMGL 451
           QQNVWMEFDL+KSRIGLAEIRCDLAGQKLGMGL
Sbjct: 421 QQNVWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 451

BLAST of MC05g1477 vs. ExPASy TrEMBL
Match: A0A1S3B5W4 (aspartic proteinase PCS1 OS=Cucumis melo OX=3656 GN=LOC103486376 PE=3 SV=1)

HSP 1 Score: 807 bits (2084), Expect = 1.90e-293
Identity = 404/453 (89.18%), Postives = 425/453 (93.82%), Query Frame = 0

Query: 1   MRDH-IAFNFSNSPFLKSCFFFFLCTLICGFQNTKLCSSLNPALVLPLKTQVIPPESVRR 60
           MR + +AF+ +N  FLKSCF FF C L   F N  LCSS+NPALVLPLKTQVIPPESVRR
Sbjct: 1   MRGYCLAFDSANIKFLKSCFLFFFCILFSVFYNIDLCSSVNPALVLPLKTQVIPPESVRR 60

Query: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNKSQNSSSNSSS-TFNPIR 120
           SPDKLPFRHN+SLTVSLTVGTPPQNVTMVIDTGSELSWLHCN SQNSSS+SSS TFNP+R
Sbjct: 61  SPDKLPFRHNISLTVSLTVGTPPQNVTMVIDTGSELSWLHCNTSQNSSSSSSSSTFNPVR 120

Query: 121 SSSYSPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADTFYIGNSG 180
           SSSYSPIPCSSSTCTDQTRDFPI  SCDSNQ CHATLSYADASSSEGNLA DTFYIGNSG
Sbjct: 121 SSSYSPIPCSSSTCTDQTRDFPIRPSCDSNQFCHATLSYADASSSEGNLATDTFYIGNSG 180

Query: 181 ISNVVFGCMDSIFSSNNEEDAKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLL 240
           I NVVFGCMDSIFSSNNEED+KNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLL
Sbjct: 181 IPNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLL 240

Query: 241 GDANYSWLGLAPLNYTPLIQMSTPLPYFDRVAYTVHLEGIKVSNKLLPIPESVFEPDHTG 300
           GDAN+SWL  APLNYTPLI+MSTPLPYFDRVAYTV LEGIKV++KLLPIPESVFEPDHTG
Sbjct: 241 GDANFSWL--APLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVAHKLLPIPESVFEPDHTG 300

Query: 301 AGQTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVLDDPNFVFQGAMDLCYRVPLNQS 360
           AGQTMVDSGTQFTFLLGPAYTALRD FLNQTAGS+R+ +DPNFVFQGAMDLCYRVP NQ+
Sbjct: 301 AGQTMVDSGTQFTFLLGPAYTALRDHFLNQTAGSLRLYEDPNFVFQGAMDLCYRVPTNQT 360

Query: 361 RLPPLPAVTLVFRGAEMTVSGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLH 420
           RLPPLP+VTLVFRGAEMTV+GDRILYRVPGE RGNDSIHCFTFGNSDLLGVEA+VIGHLH
Sbjct: 361 RLPPLPSVTLVFRGAEMTVTGDRILYRVPGERRGNDSIHCFTFGNSDLLGVEAFVIGHLH 420

Query: 421 QQNVWMEFDLQKSRIGLAEIRCDLAGQKLGMGL 451
           QQNVWMEFDL+KSRIGLAEIRCDLAGQKLGMGL
Sbjct: 421 QQNVWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 451

BLAST of MC05g1477 vs. TAIR 10
Match: AT5G02190.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 575.9 bits (1483), Expect = 2.9e-164
Identity = 291/419 (69.45%), Postives = 340/419 (81.15%), Query Frame = 0

Query: 37  SSLNPALVLPLKTQVIPPESVRRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELS 96
           SS +  LVLPLKT++ P +   R  DKL F HNV+LTV+LTVGTPPQN++MVIDTGSELS
Sbjct: 40  SSSSQTLVLPLKTRITPTD--HRPTDKLHFHHNVTLTVTLTVGTPPQNISMVIDTGSELS 99

Query: 97  WLHCNKSQNSSSNSSSTFNPIRSSSYSPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLS 156
           WL CN+S N   N  + F+P RSSSYSPIPCSS TC  +TRDF IPASCDS++LCHATLS
Sbjct: 100 WLRCNRSSN--PNPVNNFDPTRSSSYSPIPCSSPTCRTRTRDFLIPASCDSDKLCHATLS 159

Query: 157 YADASSSEGNLAADTFYIGNS-GISNVVFGCMDSIFSSNNEEDAKNTGLMGMNRGSLSFV 216
           YADASSSEGNLAA+ F+ GNS   SN++FGCM S+  S+ EED K TGL+GMNRGSLSF+
Sbjct: 160 YADASSSEGNLAAEIFHFGNSTNDSNLIFGCMGSVSGSDPEEDTKTTGLLGMNRGSLSFI 219

Query: 217 SQMGFPKFSYCIS-EYDFSGLLLLGDANYSWLGLAPLNYTPLIQMSTPLPYFDRVAYTVH 276
           SQMGFPKFSYCIS   DF G LLLGD+N++W  L PLNYTPLI++STPLPYFDRVAYTV 
Sbjct: 220 SQMGFPKFSYCISGTDDFPGFLLLGDSNFTW--LTPLNYTPLIRISTPLPYFDRVAYTVQ 279

Query: 277 LEGIKVSNKLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIR 336
           L GIKV+ KLLPIP+SV  PDHTGAGQTMVDSGTQFTFLLGP YTALR  FLN+T G + 
Sbjct: 280 LTGIKVNGKLLPIPKSVLVPDHTGAGQTMVDSGTQFTFLLGPVYTALRSHFLNRTNGILT 339

Query: 337 VLDDPNFVFQGAMDLCYR---VPLNQSRLPPLPAVTLVFRGAEMTVSGDRILYRVPGEIR 396
           V +DP+FVFQG MDLCYR   V +    L  LP V+LVF GAE+ VSG  +LYRVP    
Sbjct: 340 VYEDPDFVFQGTMDLCYRISPVRIRSGILHRLPTVSLVFEGAEIAVSGQPLLYRVPHLTV 399

Query: 397 GNDSIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLQKSRIGLAEIRCDLAGQKLGMG 451
           GNDS++CFTFGNSDL+G+EAYVIGH HQQN+W+EFDLQ+SRIGLA + CD++GQ+LG+G
Sbjct: 400 GNDSVYCFTFGNSDLMGMEAYVIGHHHQQNMWIEFDLQRSRIGLAPVECDVSGQRLGIG 452

BLAST of MC05g1477 vs. TAIR 10
Match: AT2G39710.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 543.5 bits (1399), Expect = 1.6e-154
Identity = 273/417 (65.47%), Postives = 326/417 (78.18%), Query Frame = 0

Query: 37  SSLNPALVLPLKTQVIPPESVRRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELS 96
           SS N  L+  LKTQ +P    + S DKL FRHNV+LTV+L VG PPQN++MV+DTGSELS
Sbjct: 34  SSTNQTLLFSLKTQKLP----QSSSDKLSFRHNVTLTVTLAVGDPPQNISMVLDTGSELS 93

Query: 97  WLHCNKSQNSSSNSSSTFNPIRSSSYSPIPCSSSTCTDQTRDFPIPASCD-SNQLCHATL 156
           WLHC K    S N  S FNP+ SS+YSP+PCSS  C  +TRD PIPASCD    LCH  +
Sbjct: 94  WLHCKK----SPNLGSVFNPVSSSTYSPVPCSSPICRTRTRDLPIPASCDPKTHLCHVAI 153

Query: 157 SYADASSSEGNLAADTFYIGNSGISNVVFGCMDSIFSSNNEEDAKNTGLMGMNRGSLSFV 216
           SYADA+S EGNLA +TF IG+      +FGCMDS  SSN+EEDAK+TGLMGMNRGSLSFV
Sbjct: 154 SYADATSIEGNLAHETFVIGSVTRPGTLFGCMDSGLSSNSEEDAKSTGLMGMNRGSLSFV 213

Query: 217 SQMGFPKFSYCISEYDFSGLLLLGDANYSWLGLAPLNYTPLIQMSTPLPYFDRVAYTVHL 276
           +Q+GF KFSYCIS  D SG LLLGDA+YSWLG  P+ YTPL+  STPLPYFDRVAYTV L
Sbjct: 214 NQLGFSKFSYCISGSDSSGFLLLGDASYSWLG--PIQYTPLVLQSTPLPYFDRVAYTVQL 273

Query: 277 EGIKVSNKLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRV 336
           EGI+V +K+L +P+SVF PDHTGAGQTMVDSGTQFTFL+GP YTAL++EF+ QT   +R+
Sbjct: 274 EGIRVGSKILSLPKSVFVPDHTGAGQTMVDSGTQFTFLMGPVYTALKNEFITQTKSVLRL 333

Query: 337 LDDPNFVFQGAMDLCYRV-PLNQSRLPPLPAVTLVFRGAEMTVSGDRILYRVPGE-IRGN 396
           +DDP+FVFQG MDLCY+V    +     LP V+L+FRGAEM+VSG ++LYRV G    G 
Sbjct: 334 VDDPDFVFQGTMDLCYKVGSTTRPNFSGLPMVSLMFRGAEMSVSGQKLLYRVNGAGSEGK 393

Query: 397 DSIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLQKSRIGLA-EIRCDLAGQKLGM 450
           + ++CFTFGNSDLLG+EA+VIGH HQQNVWMEFDL KSR+G A  +RCDLA Q+LG+
Sbjct: 394 EEVYCFTFGNSDLLGIEAFVIGHHHQQNVWMEFDLAKSRVGFAGNVRCDLASQRLGL 440

BLAST of MC05g1477 vs. TAIR 10
Match: AT1G66180.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 246.5 bits (628), Expect = 4.0e-65
Identity = 164/453 (36.20%), Postives = 238/453 (52.54%), Query Frame = 0

Query: 12  SPFLKSCFFFFLCTLICGFQNTKLCSSLNPALVLPLKTQVIPPESV-----RRSPD---- 71
           SP  K  FFFF    +    +T L   L P   LP+ T             R++P     
Sbjct: 2   SPIPKPLFFFFFLNYVS--LSTSLSLHL-PLTSLPISTTTNSHRFTTSLLSRKNPSPSSP 61

Query: 72  ----KLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNKSQNSSSNSSSTFNPIR 131
               +  F+++++L +SL +GTPPQ   MV+DTGS+LSW+ C++ +      +S F+P  
Sbjct: 62  PYNFRSRFKYSMALIISLPIGTPPQAQQMVLDTGSQLSWIQCHRKKLPPKPKTS-FDPSL 121

Query: 132 SSSYSPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADTFYIGNSG 191
           SSS+S +PCS   C  +  DF +P SCDSN+LCH +  YAD + +EGNL  +     N+ 
Sbjct: 122 SSSFSTLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKITFSNTE 181

Query: 192 IS-NVVFGCMDSIFSSNNEEDAKNTGLMGMNRGSLSFVSQMGFPKFSYCISE------YD 251
           I+  ++ GC          E + + G++GMNRG LSFVSQ    KFSYCI        + 
Sbjct: 182 ITPPLILGCA--------TESSDDRGILGMNRGRLSFVSQAKISKFSYCIPPKSNRPGFT 241

Query: 252 FSGLLLLGDANYSWLGLAPLNYTPLIQM--STPLPYFDRVAYTVHLEGIKVSNKLLPIPE 311
            +G   LGD   S        Y  L+    S  +P  D +AYTV + GI+   K L I  
Sbjct: 242 PTGSFYLGDNPNS----HGFKYVSLLTFPESQRMPNLDPLAYTVPMIGIRFGLKKLNISG 301

Query: 312 SVFEPDHTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVLDDPNFVFQGAMDL 371
           SVF PD  G+GQTMVDSG++FT L+  AY  +R E + +    ++      +V+ G  D+
Sbjct: 302 SVFRPDAGGSGQTMVDSGSEFTHLVDAAYDKVRAEIMTRVGRRLK----KGYVYGGTADM 361

Query: 372 CYRVPLNQSRLPPL--PAVTLVFRGAEMTVSGDRILYRVPGEIRGNDSIHCFTFGNSDLL 431
           C+    N + +P L    V +  RG E+ V  +R+L  V G       IHC   G S +L
Sbjct: 362 CF--DGNVAMIPRLIGDLVFVFTRGVEILVPKERVLVNVGG------GIHCVGIGRSSML 421

Query: 432 GVEAYVIGHLHQQNVWMEFDLQKSRIGLAEIRC 441
           G  + +IG++HQQN+W+EFD+   R+G A+  C
Sbjct: 422 GAASNIIGNVHQQNLWVEFDVTNRRVGFAKADC 426

BLAST of MC05g1477 vs. TAIR 10
Match: AT5G37540.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 235.3 bits (599), Expect = 9.3e-62
Identity = 163/461 (35.36%), Postives = 241/461 (52.28%), Query Frame = 0

Query: 14  FLKSCF-FFFLCTLICGFQNTKLCSSLNPALVLPLKTQVIPPES----------VRR--S 73
           FLK  + FFF C       +  L  S + +L  PL +  + P +           RR  S
Sbjct: 9   FLKLLYIFFFFC------YSVSLSWSSSLSLHFPLTSLRLTPTTNSSSFKTSLLSRRNPS 68

Query: 74  PDKLPF------RHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCN--KSQNSSSNSSS 133
           P   P+      +++++L +SL +GTP Q+  +V+DTGS+LSW+ C+  K +      ++
Sbjct: 69  PPSSPYTFRSNIKYSMALILSLPIGTPSQSQELVLDTGSQLSWIQCHPKKIKKPLPPPTT 128

Query: 134 TFNPIRSSSYSPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADTF 193
           +F+P  SSS+S +PCS   C  +  DF +P SCDSN+LCH +  YAD + +EGNL  + F
Sbjct: 129 SFDPSLSSSFSDLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKF 188

Query: 194 YIGNSGIS-NVVFGCMDSIFSSNNEEDAKNTGLMGMNRGSLSFVSQMGFPKFSYCI-SEY 253
              NS  +  ++ GC         +E     G++GMN G LSF+SQ    KFSYCI +  
Sbjct: 189 TFSNSQTTPPLILGCA--------KESTDEKGILGMNLGRLSFISQAKISKFSYCIPTRS 248

Query: 254 DFSGLLLLGDANYSWLGLAP----LNYTPLI--QMSTPLPYFDRVAYTVHLEGIKVSNKL 313
           +  GL   G     +LG  P      Y  L+    S  +P  D +AYTV L+GI++  K 
Sbjct: 249 NRPGLASTGS---FYLGDNPNSRGFKYVSLLTFPQSQRMPNLDPLAYTVPLQGIRIGQKR 308

Query: 314 LPIPESVFEPDHTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVLDDPNFVFQ 373
           L IP SVF PD  G+GQTMVDSG++FT L+  AY  +++E +      ++      +V+ 
Sbjct: 309 LNIPGSVFRPDAGGSGQTMVDSGSEFTHLVDVAYDKVKEEIVRLVGSRLK----KGYVYG 368

Query: 374 GAMDLCYRVPLNQSRLPPLPAVTLVF---RGAEMTVSGDRILYRVPGEIRGNDSIHCFTF 433
              D+C+    N S         LVF   RG E+ V    +L  V G       IHC   
Sbjct: 369 STADMCF--DGNHSMEIGRLIGDLVFEFGRGVEILVEKQSLLVNVGG------GIHCVGI 428

Query: 434 GNSDLLGVEAYVIGHLHQQNVWMEFDLQKSRIGLAEIRCDL 443
           G S +LG  + +IG++HQQN+W+EFD+   R+G ++  C L
Sbjct: 429 GRSSMLGAASNIIGNVHQQNLWVEFDVTNRRVGFSKAECRL 440

BLAST of MC05g1477 vs. TAIR 10
Match: AT3G25700.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 146.4 bits (368), Expect = 5.7e-35
Identity = 135/431 (31.32%), Postives = 198/431 (45.94%), Query Frame = 0

Query: 40  NPALVLPLKTQVIPPESVRRSPDKLPFRHNVSLT----------VSLTVGTPPQNVTMVI 99
           +P   L L T+ +   S+RR P  +PF  +  ++          V L +G PPQ++ ++ 
Sbjct: 44  SPTQALALDTRRLHFLSLRRKP--IPFVKSPVVSGAASGSGQYFVDLRIGQPPQSLLLIA 103

Query: 100 DTGSELSWLHCNKSQNSSSNSSST-FNPIRSSSYSPIPCSSSTC--TDQTRDFPIPASCD 159
           DTGS+L W+ C+  +N S +S +T F P  SS++SP  C    C    +    PI     
Sbjct: 104 DTGSDLVWVKCSACRNCSHHSPATVFFPRHSSTFSPAHCYDPVCRLVPKPDRAPICNHTR 163

Query: 160 SNQLCHATLSYADASSSEGNLAADTFYIGNSG-----ISNVVFGCMDSIFSSNNEEDAKN 219
            +  CH    YAD S + G  A +T  +  S      + +V FGC   I   +    + N
Sbjct: 164 IHSTCHYEYGYADGSLTSGLFARETTSLKTSSGKEARLKSVAFGCGFRISGQSVSGTSFN 223

Query: 220 --TGLMGMNRGSLSFVSQMG---FPKFSYCISEYDFS----GLLLLGDANYSWLGLAPLN 279
              G+MG+ RG +SF SQ+G     KFSYC+ +Y  S      L++G+      G++ L 
Sbjct: 224 GANGVMGLGRGPISFASQLGRRFGNKFSYCLMDYTLSPPPTSYLIIGNGGD---GISKLF 283

Query: 280 YTPLIQMSTPL-PYFDRVAYTVHLEGIKVSNKLLPIPESVFEPDHTGAGQTMVDSGTQFT 339
           +TPL  ++ PL P F    Y V L+ + V+   L I  S++E D +G G T+VDSGT   
Sbjct: 284 FTPL--LTNPLSPTF----YYVKLKSVFVNGAKLRIDPSIWEIDDSGNGGTVVDSGTTLA 343

Query: 340 FLLGPAYTALRDEFLNQTAGSIRVLDDPNFVFQGAMDLCYRVPLNQSRLPPLPAVTLVFR 399
           FL  PAY ++      +    I     P F      DLC  V         LP +   F 
Sbjct: 344 FLAEPAYRSVIAAVRRRVKLPIADALTPGF------DLCVNVSGVTKPEKILPRLKFEFS 403

Query: 400 GAEMTVSGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLQKS 443
           G  + V   R  +     I   + I C    + D   V   VIG+L QQ    EFD  +S
Sbjct: 404 GGAVFVPPPRNYF-----IETEEQIQCLAIQSVD-PKVGFSVIGNLMQQGFLFEFDRDRS 451