MC01g1128 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC01g1128
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionS-adenosyl-L-methionine-dependent methyltransferases superfamily protein
LocationMC01: 16639789 .. 16641131 (-)
RNA-Seq ExpressionMC01g1128
SyntenyMC01g1128
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGCAAAAGGTAATATCTATGACCCGGCCAATCGTGAAGGAAGCCATAACCAATTTCTTCTGTCGCTCCACAAATTTTCCGACCACCTTAACTACAGCAGATTTAGGTTGCTCTTCAGGATCGAACACTCTCATGTTTGTCTCCGATCTGATCAAACAAGTGGAGGACATTCGTCACAAGTTTCACAAAAAGCCTCTAGAATATCAAGTTTTCTTAAATGACCTCCATGGGAATGATTTCAATGCCATTTTCACATCTTTGCCAAGTTTTGTGGAGGATTTGAAGACCCAACTTGGAGGTGGTCTTGGTCCATGTTTCTTCAATGGTGTTCCGGGTTCTTTCTACGGCAGGCTTTTTCCTACCAAAAGTTTGCATTTTGTTTATTCTTCTTATAGTCTTCATTGGCTCTCTCAGGTAATTTTTATTAATATTCATGGAGTGACATCTCAAAATCAATTGGTAATGAGAGAAGTAGTTCATGTCTATTATAAACTATAGTAAAGTCCATTTCTTTTACGTGGAATCCTTAGCAATTTTCAAGTTATTAGGGATCCTAACTCCTACTAGTTATTGGTGTGTAGGCAAATATTTTAATTTCTTTCCAAAATCTTAATTTTGCATAGTGCAGGTTCCAAAAGGGATAGAGAATAACAAAGGAGAGATTTTCATGGGCAGTACAAGTCCAAAGAGTGTGCTTGAGGCTTATTACAAGCAATTTCAGAAGGATTTTTCAATGTTTCTGAAGAGTCGTGCAGAAGAATTAGCAATTGGAGGACGTATGGTTCTAACAATGATAGGAAGAACAAGTGAAGACTCGTCTAGCCAAGAGTATTGTTATGTTTGGGGTTTCTTGTCTTTAGCTCTCAATAACATGGTTGCTGAGGTAAAGTATATATATATATATACAATATTTTCCCCAAGTTTTGATGATTAACAAAAAAGAAAAAGAAAAAGAAAAGGATATTGATGCATTTCTCGATCAATCTAAATTACTGCAGGGAATTGTTGGAGCAAAAAAAGTTGACTCGTTCAATATTCCAAACTATATGCCATCTCCATCGGAAATGAAAGGTGAAATCTTAAATGAAGGAAGCTTCACTATTGATCGTCTTGAAGTTTCGAGAATTAATTGGGATTTCTACGATACAGAATCACACCAACCAAATATATTTGTTGACAGTGGCTACTATATTACTCAAAATATTAGATCTGTAATTGAACCACTACTAATTTGTCACTTTGGAGAAACCATTATCGAAGAACTTTTCTATCGATATAGAAAAATTATTGATAATCACGTGTCAAAAAACAAGATTGAGTTTGTCAATCTCACCGTTTCTCTT

mRNA sequence

CAGCAAAAGGTAATATCTATGACCCGGCCAATCGTGAAGGAAGCCATAACCAATTTCTTCTGTCGCTCCACAAATTTTCCGACCACCTTAACTACAGCAGATTTAGGTTGCTCTTCAGGATCGAACACTCTCATGTTTGTCTCCGATCTGATCAAACAAGTGGAGGACATTCGTCACAAGTTTCACAAAAAGCCTCTAGAATATCAAGTTTTCTTAAATGACCTCCATGGGAATGATTTCAATGCCATTTTCACATCTTTGCCAAGTTTTGTGGAGGATTTGAAGACCCAACTTGGAGGTGGTCTTGGTCCATGTTTCTTCAATGGTGTTCCGGGTTCTTTCTACGGCAGGCTTTTTCCTACCAAAAGTTTGCATTTTGTTTATTCTTCTTATAGTCTTCATTGGCTCTCTCAGGTTCCAAAAGGGATAGAGAATAACAAAGGAGAGATTTTCATGGGCAGTACAAGTCCAAAGAGTGTGCTTGAGGCTTATTACAAGCAATTTCAGAAGGATTTTTCAATGTTTCTGAAGAGTCGTGCAGAAGAATTAGCAATTGGAGGACGTATGGTTCTAACAATGATAGGAAGAACAAGTGAAGACTCGTCTAGCCAAGAGTATTGTTATGTTTGGGGTTTCTTGTCTTTAGCTCTCAATAACATGGTTGCTGAGGGAATTGTTGGAGCAAAAAAAGTTGACTCGTTCAATATTCCAAACTATATGCCATCTCCATCGGAAATGAAAGGTGAAATCTTAAATGAAGGAAGCTTCACTATTGATCGTCTTGAAGTTTCGAGAATTAATTGGGATTTCTACGATACAGAATCACACCAACCAAATATATTTGTTGACAGTGGCTACTATATTACTCAAAATATTAGATCTGTAATTGAACCACTACTAATTTGTCACTTTGGAGAAACCATTATCGAAGAACTTTTCTATCGATATAGAAAAATTATTGATAATCACGTGTCAAAAAACAAGATTGAGTTTGTCAATCTCACCGTTTCTCTT

Coding sequence (CDS)

CAGCAAAAGGTAATATCTATGACCCGGCCAATCGTGAAGGAAGCCATAACCAATTTCTTCTGTCGCTCCACAAATTTTCCGACCACCTTAACTACAGCAGATTTAGGTTGCTCTTCAGGATCGAACACTCTCATGTTTGTCTCCGATCTGATCAAACAAGTGGAGGACATTCGTCACAAGTTTCACAAAAAGCCTCTAGAATATCAAGTTTTCTTAAATGACCTCCATGGGAATGATTTCAATGCCATTTTCACATCTTTGCCAAGTTTTGTGGAGGATTTGAAGACCCAACTTGGAGGTGGTCTTGGTCCATGTTTCTTCAATGGTGTTCCGGGTTCTTTCTACGGCAGGCTTTTTCCTACCAAAAGTTTGCATTTTGTTTATTCTTCTTATAGTCTTCATTGGCTCTCTCAGGTTCCAAAAGGGATAGAGAATAACAAAGGAGAGATTTTCATGGGCAGTACAAGTCCAAAGAGTGTGCTTGAGGCTTATTACAAGCAATTTCAGAAGGATTTTTCAATGTTTCTGAAGAGTCGTGCAGAAGAATTAGCAATTGGAGGACGTATGGTTCTAACAATGATAGGAAGAACAAGTGAAGACTCGTCTAGCCAAGAGTATTGTTATGTTTGGGGTTTCTTGTCTTTAGCTCTCAATAACATGGTTGCTGAGGGAATTGTTGGAGCAAAAAAAGTTGACTCGTTCAATATTCCAAACTATATGCCATCTCCATCGGAAATGAAAGGTGAAATCTTAAATGAAGGAAGCTTCACTATTGATCGTCTTGAAGTTTCGAGAATTAATTGGGATTTCTACGATACAGAATCACACCAACCAAATATATTTGTTGACAGTGGCTACTATATTACTCAAAATATTAGATCTGTAATTGAACCACTACTAATTTGTCACTTTGGAGAAACCATTATCGAAGAACTTTTCTATCGATATAGAAAAATTATTGATAATCACGTGTCAAAAAACAAGATTGAGTTTGTCAATCTCACCGTTTCTCTT

Protein sequence

QQKVISMTRPIVKEAITNFFCRSTNFPTTLTTADLGCSSGSNTLMFVSDLIKQVEDIRHKFHKKPLEYQVFLNDLHGNDFNAIFTSLPSFVEDLKTQLGGGLGPCFFNGVPGSFYGRLFPTKSLHFVYSSYSLHWLSQVPKGIENNKGEIFMGSTSPKSVLEAYYKQFQKDFSMFLKSRAEELAIGGRMVLTMIGRTSEDSSSQEYCYVWGFLSLALNNMVAEGIVGAKKVDSFNIPNYMPSPSEMKGEILNEGSFTIDRLEVSRINWDFYDTESHQPNIFVDSGYYITQNIRSVIEPLLICHFGETIIEELFYRYRKIIDNHVSKNKIEFVNLTVSL
Homology
BLAST of MC01g1128 vs. ExPASy Swiss-Prot
Match: Q84UB4 (S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 2 OS=Petunia hybrida OX=4102 GN=BSMT2 PE=1 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 1.8e-102
Identity = 185/338 (54.73%), Postives = 248/338 (73.37%), Query Frame = 0

Query: 1   QQKVISMTRPIVKEAITNFFCRSTNFPTTLTTADLGCSSGSNTLMFVSDLIKQVEDIRHK 60
           QQKVI MT+PI ++A+ + +  S+ FP TL  ADLGCS G+NT + VS L+K VE  R K
Sbjct: 25  QQKVILMTKPITEQAMIDLY--SSLFPETLCIADLGCSLGANTFLVVSQLVKIVEKERKK 84

Query: 61  FHKKPLEYQVFLNDLHGNDFNAIFTSLPSFVEDLKTQLGGGLGPCFFNGVPGSFYGRLFP 120
              K  E+    NDL GNDFN +F SL +F EDL+  +G   GPCFF+GVPGSFY RLFP
Sbjct: 85  HGFKSPEFYFHFNDLPGNDFNTLFQSLGAFQEDLRKHIGESFGPCFFSGVPGSFYTRLFP 144

Query: 121 TKSLHFVYSSYSLHWLSQVPKGIENNKGEIFMGSTSPKSVLEAYYKQFQKDFSMFLKSRA 180
           +KSLHFVYSSYSL WLSQVP GIENNKG I+M  TSP SV++AYYKQ++ DFS FLK R+
Sbjct: 145 SKSLHFVYSSYSLMWLSQVPNGIENNKGNIYMARTSPLSVIKAYYKQYEIDFSNFLKYRS 204

Query: 181 EELAIGGRMVLTMIGRTSEDSSSQEYCYVWGFLSLALNNMVAEGIVGAKKVDSFNIPNYM 240
           EEL  GG+MVLT++GR SED +S+E CY+W  L++ALN +V EG++  +KVD+FNIP Y 
Sbjct: 205 EELMKGGKMVLTLLGRESEDPTSKECCYIWELLAMALNKLVEEGLIKEEKVDAFNIPQYT 264

Query: 241 PSPSEMKGEILNEGSFTIDRLEVSRINWDFYDTESHQPNIFVDSGYYITQNIRSVIEPLL 300
           PSP+E+K  +  EGSFTI+RLE SR++W+  + E        + GY +++ +R+V EPLL
Sbjct: 265 PSPAEVKYIVEKEGSFTINRLETSRVHWNASNNEK-------NGGYNVSRCMRAVAEPLL 324

Query: 301 ICHFGETIIEELFYRYRKIIDNHVSKNKIEFVNLTVSL 339
           + HF + +++ +F++Y +II + +SK K EF+N+ VSL
Sbjct: 325 VSHFDKELMDLVFHKYEEIISDCMSKEKTEFINVIVSL 353

BLAST of MC01g1128 vs. ExPASy Swiss-Prot
Match: A4ZDG8 (S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 3 OS=Petunia hybrida OX=4102 GN=BSMT3 PE=2 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 6.7e-102
Identity = 184/338 (54.44%), Postives = 247/338 (73.08%), Query Frame = 0

Query: 1   QQKVISMTRPIVKEAITNFFCRSTNFPTTLTTADLGCSSGSNTLMFVSDLIKQVEDIRHK 60
           QQKVI MT+PI ++A+ + +  S+ FP TL  ADLGCS G+NT + VS ++K VE  R K
Sbjct: 25  QQKVILMTKPITEQAMIDLY--SSLFPETLCIADLGCSLGANTFLVVSQIVKIVEKERKK 84

Query: 61  FHKKPLEYQVFLNDLHGNDFNAIFTSLPSFVEDLKTQLGGGLGPCFFNGVPGSFYGRLFP 120
              K  E+    NDL GNDFN +F SL +F EDL+  +G   GPCFF+GVPGSFY RLFP
Sbjct: 85  HGFKSPEFYFHFNDLPGNDFNTLFQSLGAFQEDLRKHIGESFGPCFFSGVPGSFYTRLFP 144

Query: 121 TKSLHFVYSSYSLHWLSQVPKGIENNKGEIFMGSTSPKSVLEAYYKQFQKDFSMFLKSRA 180
           +KSLHFVYSSYSL WLSQVP GIENNKG I+M  TSP SV++AYYKQ++ DFS FLK R+
Sbjct: 145 SKSLHFVYSSYSLMWLSQVPNGIENNKGNIYMARTSPLSVIKAYYKQYEIDFSNFLKYRS 204

Query: 181 EELAIGGRMVLTMIGRTSEDSSSQEYCYVWGFLSLALNNMVAEGIVGAKKVDSFNIPNYM 240
           EEL  GG+MVLT++GR SED +S+E CY+W  L++ALN +V EG++  +KVD+FNIP Y 
Sbjct: 205 EELMKGGKMVLTLLGRESEDPTSKECCYIWELLAMALNELVKEGLIKEEKVDAFNIPQYT 264

Query: 241 PSPSEMKGEILNEGSFTIDRLEVSRINWDFYDTESHQPNIFVDSGYYITQNIRSVIEPLL 300
           PSP+E+K  +  EGSFTI+RLE SR++W       +  N   + GY +++ +R+V EPLL
Sbjct: 265 PSPAEVKYLVEKEGSFTINRLETSRVHW-------NASNNVKNGGYNVSRCMRAVAEPLL 324

Query: 301 ICHFGETIIEELFYRYRKIIDNHVSKNKIEFVNLTVSL 339
           + HF + +++ +F++Y +II + +SK K EF+N+ VSL
Sbjct: 325 VSHFDKELMDLVFHKYEEIISDCMSKEKTEFINVIVSL 353

BLAST of MC01g1128 vs. ExPASy Swiss-Prot
Match: Q84UB5 (S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 1 OS=Petunia hybrida OX=4102 GN=BSMT1 PE=1 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 1.1e-101
Identity = 182/338 (53.85%), Postives = 247/338 (73.08%), Query Frame = 0

Query: 1   QQKVISMTRPIVKEAITNFFCRSTNFPTTLTTADLGCSSGSNTLMFVSDLIKQVEDIRHK 60
           QQKVI MT+PI ++A+ + +  S+ FP TL  ADLGCS G+NT + VS L+K VE  R K
Sbjct: 25  QQKVILMTKPITEQAMIDLY--SSLFPETLCIADLGCSLGANTFLVVSQLVKIVEKERKK 84

Query: 61  FHKKPLEYQVFLNDLHGNDFNAIFTSLPSFVEDLKTQLGGGLGPCFFNGVPGSFYGRLFP 120
              K  E+    NDL GNDFN +F SL +F EDL+  +G   GPCFF+GVPGSFY RLFP
Sbjct: 85  HGFKSPEFYFHFNDLPGNDFNTLFQSLGAFQEDLRKHIGESFGPCFFSGVPGSFYTRLFP 144

Query: 121 TKSLHFVYSSYSLHWLSQVPKGIENNKGEIFMGSTSPKSVLEAYYKQFQKDFSMFLKSRA 180
           +KSLHFVYSSYSL WLSQVP GIENNKG I+M  TSP SV++AYYKQ++ DFS FLK R+
Sbjct: 145 SKSLHFVYSSYSLMWLSQVPNGIENNKGNIYMARTSPLSVIKAYYKQYEIDFSNFLKYRS 204

Query: 181 EELAIGGRMVLTMIGRTSEDSSSQEYCYVWGFLSLALNNMVAEGIVGAKKVDSFNIPNYM 240
           EEL  GG+MVLT++GR SED +S+E CY+W  L++ALN +V EG++  +KVD+FNIP Y 
Sbjct: 205 EELMKGGKMVLTLLGRESEDPTSKECCYIWELLAMALNKLVEEGLIKEEKVDAFNIPQYT 264

Query: 241 PSPSEMKGEILNEGSFTIDRLEVSRINWDFYDTESHQPNIFVDSGYYITQNIRSVIEPLL 300
           PSP+E+K  +  EGSFTI+RLE SR++W+  + E        + GY +++ +R+V EPLL
Sbjct: 265 PSPAEVKYIVEKEGSFTINRLETSRVHWNASNNEK-------NGGYNVSRCMRAVAEPLL 324

Query: 301 ICHFGETIIEELFYRYRKIIDNHVSKNKIEFVNLTVSL 339
           + HF + +++ +F++Y +I+ + +SK   EF+N+ +SL
Sbjct: 325 VSHFDKELMDLVFHKYEEIVSDCMSKENTEFINVIISL 353

BLAST of MC01g1128 vs. ExPASy Swiss-Prot
Match: Q9SPV4 (Salicylate carboxymethyltransferase OS=Clarkia breweri OX=36903 GN=SAMT PE=1 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 2.2e-97
Identity = 182/339 (53.69%), Postives = 239/339 (70.50%), Query Frame = 0

Query: 1   QQKVISMTRPIVKEAITNFFCRSTNFPTTLTTADLGCSSGSNTLMFVSDLIKQVEDIRHK 60
           Q++VIS+T+PI + AIT  +   T   T L  ADLGCSSG N L  V++LIK VE++R K
Sbjct: 25  QRQVISITKPITEAAITALYSGDT-VTTRLAIADLGCSSGPNALFAVTELIKTVEELRKK 84

Query: 61  F-HKKPLEYQVFLNDLHGNDFNAIFTSLPSFVEDLKTQLGGGLGPCFFNGVPGSFYGRLF 120
              +   EYQ+FLNDL GNDFNAIF SLP    D+        G CF NGVPGSFYGRLF
Sbjct: 85  MGRENSPEYQIFLNDLPGNDFNAIFRSLP-IENDVD-------GVCFINGVPGSFYGRLF 144

Query: 121 PTKSLHFVYSSYSLHWLSQVPKGIENNKGEIFMGSTSPKSVLEAYYKQFQKDFSMFLKSR 180
           P  +LHF++SSYSL WLSQVP GIE+NKG I+M +T P+SVL AYYKQFQ+D ++FL+ R
Sbjct: 145 PRNTLHFIHSSYSLMWLSQVPIGIESNKGNIYMANTCPQSVLNAYYKQFQEDHALFLRCR 204

Query: 181 AEELAIGGRMVLTMIGRTSEDSSSQEYCYVWGFLSLALNNMVAEGIVGAKKVDSFNIPNY 240
           A+E+  GGRMVLT++GR SED +S E C +W  L++ALN MV+EG++  +K+D FNIP Y
Sbjct: 205 AQEVVPGGRMVLTILGRRSEDRASTECCLIWQLLAMALNQMVSEGLIEEEKMDKFNIPQY 264

Query: 241 MPSPSEMKGEILNEGSFTIDRLEVSRINWDFYDTESHQPNIFVDSGYYITQNIRSVIEPL 300
            PSP+E++ EIL EGSF ID +E S I W     +        + GY + + +R+V EPL
Sbjct: 265 TPSPTEVEAEILKEGSFLIDHIEASEIYWSSCTKDGDGGGSVEEEGYNVARCMRAVAEPL 324

Query: 301 LICHFGETIIEELFYRYRKIIDNHVSKNKIEFVNLTVSL 339
           L+ HFGE IIE++F+RY+ +I   +SK K +F+N+ VSL
Sbjct: 325 LLDHFGEAIIEDVFHRYKLLIIERMSKEKTKFINVIVSL 354

BLAST of MC01g1128 vs. ExPASy Swiss-Prot
Match: A0A061FBW2 (Probable jasmonic acid carboxyl methyltransferase 2 OS=Theobroma cacao OX=3641 GN=TCM_034091 PE=3 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 6.1e-87
Identity = 164/349 (46.99%), Postives = 236/349 (67.62%), Query Frame = 0

Query: 1   QQKVISMTRPIVKEAITNFFCRSTNFPTTLTTADLGCSSGSNTLMFVSDLIKQVEDIRHK 60
           Q K+IS+ +PI++EA+    C   N   ++  ADLGCSSG NTL  +S+++  V+    +
Sbjct: 25  QSKIISVGKPIIEEAVHEISC--NNLLESMGIADLGCSSGPNTLSVISEIMDMVQTTSRR 84

Query: 61  FHKKPLEYQVFLNDLHGNDFNAIFTSLPSFVEDLKTQLGGGLGPCFFNGVPGSFYGRLFP 120
             +   E++V+LNDL+ NDFN IF SLP+F   LK + G G G C+ +GV GSFYGRLFP
Sbjct: 85  LGRPVPEFRVYLNDLYSNDFNYIFMSLPAFYHRLKEEKGIGCGSCYISGVAGSFYGRLFP 144

Query: 121 TKSLHFVYSSYSLHWLSQVPKGIEN------NKGEIFMGSTSPKSVLEAYYKQFQKDFSM 180
           +KSLHFV+SS SLHWLSQVP G+E+      NKG++++  +SP+SVL AY  QFQ DFSM
Sbjct: 145 SKSLHFVHSSSSLHWLSQVPPGLESKALAPLNKGKVYISKSSPQSVLNAYSLQFQNDFSM 204

Query: 181 FLKSRAEELAIGGRMVLTMIGRTSEDSSSQEYCYVWGFLSLALNNMVAEGIVGAKKVDSF 240
           F++SR++EL  GGRMVL+ +GR S D +++E C+ W  L+ A+ ++V EG++   KVDSF
Sbjct: 205 FIESRSQELVPGGRMVLSFMGRRSTDPTTEESCHHWELLAQAIMSLVREGLIEEAKVDSF 264

Query: 241 NIPNYMPSPSEMKGEILNEGSFTIDRLEVSRINWD---FYDTESHQPNIFVDSGYYITQN 300
           N P Y P   E+K EI   GSF IDRLE   I+WD     D ++ Q  + +  G  + + 
Sbjct: 265 NAPYYAPCAEEIKVEIQKVGSFVIDRLEGFEIDWDGGAVSDVQTAQGKLLI--GQRVAKT 324

Query: 301 IRSVIEPLLICHF--GETIIEELFYRYRKIIDNHVSKNKIEFVNLTVSL 339
           IR+V+E +L  HF  G+ I+++LF RY +I+ NH+SK + +++NL +SL
Sbjct: 325 IRAVVESMLESHFGIGQDIMDDLFSRYAEIVGNHLSKTRTKYINLVISL 369

BLAST of MC01g1128 vs. NCBI nr
Match: XP_022133322.1 (salicylate carboxymethyltransferase-like [Momordica charantia])

HSP 1 Score: 685 bits (1767), Expect = 2.76e-248
Identity = 337/337 (100.00%), Postives = 337/337 (100.00%), Query Frame = 0

Query: 2   QKVISMTRPIVKEAITNFFCRSTNFPTTLTTADLGCSSGSNTLMFVSDLIKQVEDIRHKF 61
           QKVISMTRPIVKEAITNFFCRSTNFPTTLTTADLGCSSGSNTLMFVSDLIKQVEDIRHKF
Sbjct: 21  QKVISMTRPIVKEAITNFFCRSTNFPTTLTTADLGCSSGSNTLMFVSDLIKQVEDIRHKF 80

Query: 62  HKKPLEYQVFLNDLHGNDFNAIFTSLPSFVEDLKTQLGGGLGPCFFNGVPGSFYGRLFPT 121
           HKKPLEYQVFLNDLHGNDFNAIFTSLPSFVEDLKTQLGGGLGPCFFNGVPGSFYGRLFPT
Sbjct: 81  HKKPLEYQVFLNDLHGNDFNAIFTSLPSFVEDLKTQLGGGLGPCFFNGVPGSFYGRLFPT 140

Query: 122 KSLHFVYSSYSLHWLSQVPKGIENNKGEIFMGSTSPKSVLEAYYKQFQKDFSMFLKSRAE 181
           KSLHFVYSSYSLHWLSQVPKGIENNKGEIFMGSTSPKSVLEAYYKQFQKDFSMFLKSRAE
Sbjct: 141 KSLHFVYSSYSLHWLSQVPKGIENNKGEIFMGSTSPKSVLEAYYKQFQKDFSMFLKSRAE 200

Query: 182 ELAIGGRMVLTMIGRTSEDSSSQEYCYVWGFLSLALNNMVAEGIVGAKKVDSFNIPNYMP 241
           ELAIGGRMVLTMIGRTSEDSSSQEYCYVWGFLSLALNNMVAEGIVGAKKVDSFNIPNYMP
Sbjct: 201 ELAIGGRMVLTMIGRTSEDSSSQEYCYVWGFLSLALNNMVAEGIVGAKKVDSFNIPNYMP 260

Query: 242 SPSEMKGEILNEGSFTIDRLEVSRINWDFYDTESHQPNIFVDSGYYITQNIRSVIEPLLI 301
           SPSEMKGEILNEGSFTIDRLEVSRINWDFYDTESHQPNIFVDSGYYITQNIRSVIEPLLI
Sbjct: 261 SPSEMKGEILNEGSFTIDRLEVSRINWDFYDTESHQPNIFVDSGYYITQNIRSVIEPLLI 320

Query: 302 CHFGETIIEELFYRYRKIIDNHVSKNKIEFVNLTVSL 338
           CHFGETIIEELFYRYRKIIDNHVSKNKIEFVNLTVSL
Sbjct: 321 CHFGETIIEELFYRYRKIIDNHVSKNKIEFVNLTVSL 357

BLAST of MC01g1128 vs. NCBI nr
Match: XP_022132391.1 (salicylate carboxymethyltransferase-like [Momordica charantia])

HSP 1 Score: 477 bits (1227), Expect = 4.94e-166
Identity = 241/342 (70.47%), Postives = 286/342 (83.63%), Query Frame = 0

Query: 1   QQKVISMTRPIVKEAITNFFCRSTNFPTTLTTADLGCSSGSNTLMFVSDLIKQVE-DIRH 60
           Q+KVISMT PI KEAIT+FFC   NFPTTLT  DLGCS G NTLMFVS+LIKQVE +IR 
Sbjct: 20  QRKVISMTWPIAKEAITDFFCTH-NFPTTLTMVDLGCSYGQNTLMFVSNLIKQVEQNIRE 79

Query: 61  KFHKKPLEYQVFLNDLHGNDFNAIFTSLPSFVEDLKTQLGGGLGPCFFNGVPGSFYGRLF 120
           +   +PLEYQ+F NDL+GNDFN IF SLPSF++ LKTQ+G   GPCFFNGVPGSFYGRLF
Sbjct: 80  E---RPLEYQIFFNDLYGNDFNTIFRSLPSFLQHLKTQIGPDFGPCFFNGVPGSFYGRLF 139

Query: 121 PTKSLHFVYSSYSLHWLSQVPKGIENNKGEIFMGSTSPKSVLEAYYKQFQKDFSMFLKSR 180
           P KS+HF +SSYSLHWLSQVP+GIENNKG IFMGSTSP+SVLEAYY+QF+KDFSMFL+ R
Sbjct: 140 PVKSVHFFHSSYSLHWLSQVPEGIENNKGNIFMGSTSPRSVLEAYYRQFRKDFSMFLRCR 199

Query: 181 AEELAIGGRMVLTMIGRTSEDSSSQEYCYVWGFLSLALNNMVAEGIVGAKKVDSFNIPNY 240
           AEEL IGGRMVLT++GRTS D +++E  YVW F +LALN+MV EGIV  +KV+SFNIPNY
Sbjct: 200 AEELVIGGRMVLTIVGRTSGDPANKECSYVWEFFTLALNSMVVEGIVEEEKVNSFNIPNY 259

Query: 241 MPSPSEMKGEILNEGSFTIDRLEVSRINWDFYDT--ESHQPNIFVDS-GYYITQNIRSVI 300
           MPSP E+K E+LNEGSFTI+RLEVSRI+W+F D   ES+  ++F+D  GY   + +RSV+
Sbjct: 260 MPSPLEVKVEVLNEGSFTINRLEVSRIDWNFQDNGIESNTSSMFMDDYGYDFVKCVRSVV 319

Query: 301 EPLLICHFGETIIEELFYRYRKIIDNHVSKNKIEFVNLTVSL 338
           EPL+I HFGE I++ELF RYRKI  +H+S  KIEFVNLT+SL
Sbjct: 320 EPLIIHHFGEAIMDELFCRYRKIAADHMSTEKIEFVNLTISL 357

BLAST of MC01g1128 vs. NCBI nr
Match: KAF3959833.1 (hypothetical protein CMV_015386 [Castanea mollissima])

HSP 1 Score: 441 bits (1134), Expect = 1.74e-151
Identity = 212/338 (62.72%), Postives = 264/338 (78.11%), Query Frame = 0

Query: 1   QQKVISMTRPIVKEAITNFFCRSTNFPTTLTTADLGCSSGSNTLMFVSDLIKQVEDIRHK 60
           QQKVIS+TRPI +EAITN +CR+   P +L  ADLGCSSG NTL  VS+LIK VE +R K
Sbjct: 45  QQKVISLTRPITEEAITNLYCRT--LPRSLAIADLGCSSGPNTLFVVSELIKVVEKLRKK 104

Query: 61  FHKKPLEYQVFLNDLHGNDFNAIFTSLPSFVEDLKTQLGGGLGPCFFNGVPGSFYGRLFP 120
              + LEYQVFLNDL GNDFN IF SLPSF + +  Q+G G GPC F+G PGSFYGRLFP
Sbjct: 105 LGHESLEYQVFLNDLPGNDFNTIFKSLPSFQKQMSCQMGPGAGPCLFSGTPGSFYGRLFP 164

Query: 121 TKSLHFVYSSYSLHWLSQVPKGIENNKGEIFMGSTSPKSVLEAYYKQFQKDFSMFLKSRA 180
           + SLHFV+SSYSL WLSQVPKG++NNKG I+M STSP S+L AYY+QFQ+DFS+FLK RA
Sbjct: 165 SNSLHFVHSSYSLQWLSQVPKGLDNNKGNIYMASTSPPSILRAYYEQFQRDFSLFLKCRA 224

Query: 181 EELAIGGRMVLTMIGRTSEDSSSQEYCYVWGFLSLALNNMVAEGIVGAKKVDSFNIPNYM 240
           EEL +GG MVLT +GR SED SS+E CY+W  L++ALN+MV+EG++   K+DSFNIP Y 
Sbjct: 225 EELIVGGGMVLTFLGRRSEDPSSKECCYIWELLAMALNDMVSEGLIEEDKMDSFNIPQYT 284

Query: 241 PSPSEMKGEILNEGSFTIDRLEVSRINWDFYDTESHQPNIFVDSGYYITQNIRSVIEPLL 300
           PSPSE+K E+L  G F+IDRLEVS + W+ YD+E +    F D GY +   +R+V EPLL
Sbjct: 285 PSPSEVKSEVLKGGYFSIDRLEVSTVKWNDYDSEFNPSIAFSDDGYCVANCMRAVAEPLL 344

Query: 301 ICHFGETIIEELFYRYRKIIDNHVSKNKIEFVNLTVSL 338
           I  FG+TII+E+F RYRKII +H+SK K +F+N+T+S+
Sbjct: 345 INQFGDTIIDEVFRRYRKIIADHISKEKTQFINVTISM 380

BLAST of MC01g1128 vs. NCBI nr
Match: XP_022133132.1 (salicylate carboxymethyltransferase-like [Momordica charantia])

HSP 1 Score: 439 bits (1129), Expect = 4.50e-151
Identity = 218/335 (65.07%), Postives = 264/335 (78.81%), Query Frame = 0

Query: 7   MTRPIVKEAITNFFCRSTNFPTTLTTADLGCSSGSNTLMFVSDLIKQVEDIRHKFHKKPL 66
           +T  IVKEAIT  +  ST+FPT LT ADLGCSSG NT M VS LIK+VEDIR + HK  +
Sbjct: 30  LTWSIVKEAITQLY--STSFPTALTIADLGCSSGPNTFMVVSKLIKEVEDIRQQLHKNSI 89

Query: 67  EYQVFLNDLHGNDFNAIFTSLPSFVEDLKTQLGGGLGPCFFNGVPGSFYGRLFPTKSLHF 126
           EYQ+FLNDLHGNDFN+IF SLPSF E+LKT +G GLGPCF  G PGSFYGRLFP KSLHF
Sbjct: 90  EYQIFLNDLHGNDFNSIFKSLPSFHENLKTHMGNGLGPCFLVGAPGSFYGRLFPGKSLHF 149

Query: 127 VYSSYSLHWLSQVPKGIENNKGEIFMGSTSPKSVLEAYYKQFQKDFSMFLKSRAEELAIG 186
           V+SS SLHWLSQVP+GIENNKG IF+  TSPK+VLEAY+KQFQKDFS+FLK RAEEL  G
Sbjct: 150 VHSSNSLHWLSQVPEGIENNKGNIFISETSPKTVLEAYHKQFQKDFSLFLKCRAEELVAG 209

Query: 187 GRMVLTMIGRTSEDSSSQEYCYVWGFLSLALNNMVAEGIVGAKKVDSFNIPNYMPSPSEM 246
           GRMVLT+  RT ED  ++E CY W  L+LA+N+MV+EGIV  +K+DSFN+P YMPS  E+
Sbjct: 210 GRMVLTISARTCEDRVNKECCYSWELLNLAVNDMVSEGIVEEEKLDSFNVPIYMPSSLEV 269

Query: 247 KGEILNEGSFTIDRLEVSRINWDFYDTESHQP---NIFVDSGYYITQNIRSVIEPLLICH 306
           + E+LNEGSF I+ L+V R+NW+ Y+ E +     N  +DSGY   + IRSV EPLL+ H
Sbjct: 270 EAEVLNEGSFIIEHLQVVRVNWNIYNNELNSDIPSNEIIDSGYNYAKCIRSVAEPLLVRH 329

Query: 307 FGETIIEELFYRYRKIIDNHVSKNKIEFVNLTVSL 338
           FGE II+ELF RYR+II++H+SK  IE ++ T+SL
Sbjct: 330 FGEDIIDELFSRYREIINDHMSKENIENISFTISL 362

BLAST of MC01g1128 vs. NCBI nr
Match: XP_023918353.1 (salicylate carboxymethyltransferase-like [Quercus suber])

HSP 1 Score: 437 bits (1124), Expect = 2.50e-150
Identity = 207/338 (61.24%), Postives = 264/338 (78.11%), Query Frame = 0

Query: 1   QQKVISMTRPIVKEAITNFFCRSTNFPTTLTTADLGCSSGSNTLMFVSDLIKQVEDIRHK 60
           QQKVISMT PI +EAI N +C +   P ++  ADLGCSSG NTL  VS+LIK+V+ +R K
Sbjct: 25  QQKVISMTIPIAEEAIANLYCNT--LPRSIAIADLGCSSGPNTLFVVSELIKEVDKLRQK 84

Query: 61  FHKKPLEYQVFLNDLHGNDFNAIFTSLPSFVEDLKTQLGGGLGPCFFNGVPGSFYGRLFP 120
                 EYQVFLNDL GNDFN IF SLPSF +++  QLG G GPC F+G PGSFYGRLFP
Sbjct: 85  LGHDSPEYQVFLNDLPGNDFNTIFKSLPSFQKEMSCQLGPGAGPCLFSGTPGSFYGRLFP 144

Query: 121 TKSLHFVYSSYSLHWLSQVPKGIENNKGEIFMGSTSPKSVLEAYYKQFQKDFSMFLKSRA 180
           +  LHF++SSYSLHWLSQVP+G++NNKG I+M STSP  V +AYY+QFQ+DFS+FLK RA
Sbjct: 145 SNCLHFIHSSYSLHWLSQVPEGVDNNKGNIYMASTSPPCVFKAYYEQFQRDFSIFLKCRA 204

Query: 181 EELAIGGRMVLTMIGRTSEDSSSQEYCYVWGFLSLALNNMVAEGIVGAKKVDSFNIPNYM 240
           EEL  GGRMVLT++GR SED SS+E CY++  +++ALN+MV+EG++   KVDSFNIPNY 
Sbjct: 205 EELVAGGRMVLTLLGRRSEDPSSKECCYIYELMAMALNDMVSEGLIEEDKVDSFNIPNYT 264

Query: 241 PSPSEMKGEILNEGSFTIDRLEVSRINWDFYDTESHQPNIFVDSGYYITQNIRSVIEPLL 300
           PSPSE+K E+L  G F+IDRLEVS +NW+ YD E +  N F + GY + + IR+V EPLL
Sbjct: 265 PSPSELKFEVLKGGDFSIDRLEVSEVNWNIYDGEFNTSNGFSNDGYGVAKYIRAVAEPLL 324

Query: 301 ICHFGETIIEELFYRYRKIIDNHVSKNKIEFVNLTVSL 338
           + HFG+ II+E+F+RY KIID+ +SK K +F+NLT+S+
Sbjct: 325 VTHFGDAIIDEVFHRYGKIIDDRISKEKTQFINLTISV 360

BLAST of MC01g1128 vs. ExPASy TrEMBL
Match: A0A6J1BWC5 (salicylate carboxymethyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111005930 PE=4 SV=1)

HSP 1 Score: 685 bits (1767), Expect = 1.34e-248
Identity = 337/337 (100.00%), Postives = 337/337 (100.00%), Query Frame = 0

Query: 2   QKVISMTRPIVKEAITNFFCRSTNFPTTLTTADLGCSSGSNTLMFVSDLIKQVEDIRHKF 61
           QKVISMTRPIVKEAITNFFCRSTNFPTTLTTADLGCSSGSNTLMFVSDLIKQVEDIRHKF
Sbjct: 21  QKVISMTRPIVKEAITNFFCRSTNFPTTLTTADLGCSSGSNTLMFVSDLIKQVEDIRHKF 80

Query: 62  HKKPLEYQVFLNDLHGNDFNAIFTSLPSFVEDLKTQLGGGLGPCFFNGVPGSFYGRLFPT 121
           HKKPLEYQVFLNDLHGNDFNAIFTSLPSFVEDLKTQLGGGLGPCFFNGVPGSFYGRLFPT
Sbjct: 81  HKKPLEYQVFLNDLHGNDFNAIFTSLPSFVEDLKTQLGGGLGPCFFNGVPGSFYGRLFPT 140

Query: 122 KSLHFVYSSYSLHWLSQVPKGIENNKGEIFMGSTSPKSVLEAYYKQFQKDFSMFLKSRAE 181
           KSLHFVYSSYSLHWLSQVPKGIENNKGEIFMGSTSPKSVLEAYYKQFQKDFSMFLKSRAE
Sbjct: 141 KSLHFVYSSYSLHWLSQVPKGIENNKGEIFMGSTSPKSVLEAYYKQFQKDFSMFLKSRAE 200

Query: 182 ELAIGGRMVLTMIGRTSEDSSSQEYCYVWGFLSLALNNMVAEGIVGAKKVDSFNIPNYMP 241
           ELAIGGRMVLTMIGRTSEDSSSQEYCYVWGFLSLALNNMVAEGIVGAKKVDSFNIPNYMP
Sbjct: 201 ELAIGGRMVLTMIGRTSEDSSSQEYCYVWGFLSLALNNMVAEGIVGAKKVDSFNIPNYMP 260

Query: 242 SPSEMKGEILNEGSFTIDRLEVSRINWDFYDTESHQPNIFVDSGYYITQNIRSVIEPLLI 301
           SPSEMKGEILNEGSFTIDRLEVSRINWDFYDTESHQPNIFVDSGYYITQNIRSVIEPLLI
Sbjct: 261 SPSEMKGEILNEGSFTIDRLEVSRINWDFYDTESHQPNIFVDSGYYITQNIRSVIEPLLI 320

Query: 302 CHFGETIIEELFYRYRKIIDNHVSKNKIEFVNLTVSL 338
           CHFGETIIEELFYRYRKIIDNHVSKNKIEFVNLTVSL
Sbjct: 321 CHFGETIIEELFYRYRKIIDNHVSKNKIEFVNLTVSL 357

BLAST of MC01g1128 vs. ExPASy TrEMBL
Match: A0A6J1BS51 (salicylate carboxymethyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111005262 PE=4 SV=1)

HSP 1 Score: 477 bits (1227), Expect = 2.39e-166
Identity = 241/342 (70.47%), Postives = 286/342 (83.63%), Query Frame = 0

Query: 1   QQKVISMTRPIVKEAITNFFCRSTNFPTTLTTADLGCSSGSNTLMFVSDLIKQVE-DIRH 60
           Q+KVISMT PI KEAIT+FFC   NFPTTLT  DLGCS G NTLMFVS+LIKQVE +IR 
Sbjct: 20  QRKVISMTWPIAKEAITDFFCTH-NFPTTLTMVDLGCSYGQNTLMFVSNLIKQVEQNIRE 79

Query: 61  KFHKKPLEYQVFLNDLHGNDFNAIFTSLPSFVEDLKTQLGGGLGPCFFNGVPGSFYGRLF 120
           +   +PLEYQ+F NDL+GNDFN IF SLPSF++ LKTQ+G   GPCFFNGVPGSFYGRLF
Sbjct: 80  E---RPLEYQIFFNDLYGNDFNTIFRSLPSFLQHLKTQIGPDFGPCFFNGVPGSFYGRLF 139

Query: 121 PTKSLHFVYSSYSLHWLSQVPKGIENNKGEIFMGSTSPKSVLEAYYKQFQKDFSMFLKSR 180
           P KS+HF +SSYSLHWLSQVP+GIENNKG IFMGSTSP+SVLEAYY+QF+KDFSMFL+ R
Sbjct: 140 PVKSVHFFHSSYSLHWLSQVPEGIENNKGNIFMGSTSPRSVLEAYYRQFRKDFSMFLRCR 199

Query: 181 AEELAIGGRMVLTMIGRTSEDSSSQEYCYVWGFLSLALNNMVAEGIVGAKKVDSFNIPNY 240
           AEEL IGGRMVLT++GRTS D +++E  YVW F +LALN+MV EGIV  +KV+SFNIPNY
Sbjct: 200 AEELVIGGRMVLTIVGRTSGDPANKECSYVWEFFTLALNSMVVEGIVEEEKVNSFNIPNY 259

Query: 241 MPSPSEMKGEILNEGSFTIDRLEVSRINWDFYDT--ESHQPNIFVDS-GYYITQNIRSVI 300
           MPSP E+K E+LNEGSFTI+RLEVSRI+W+F D   ES+  ++F+D  GY   + +RSV+
Sbjct: 260 MPSPLEVKVEVLNEGSFTINRLEVSRIDWNFQDNGIESNTSSMFMDDYGYDFVKCVRSVV 319

Query: 301 EPLLICHFGETIIEELFYRYRKIIDNHVSKNKIEFVNLTVSL 338
           EPL+I HFGE I++ELF RYRKI  +H+S  KIEFVNLT+SL
Sbjct: 320 EPLIIHHFGEAIMDELFCRYRKIAADHMSTEKIEFVNLTISL 357

BLAST of MC01g1128 vs. ExPASy TrEMBL
Match: A0A6J1BVR9 (salicylate carboxymethyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111005805 PE=4 SV=1)

HSP 1 Score: 439 bits (1129), Expect = 2.18e-151
Identity = 218/335 (65.07%), Postives = 264/335 (78.81%), Query Frame = 0

Query: 7   MTRPIVKEAITNFFCRSTNFPTTLTTADLGCSSGSNTLMFVSDLIKQVEDIRHKFHKKPL 66
           +T  IVKEAIT  +  ST+FPT LT ADLGCSSG NT M VS LIK+VEDIR + HK  +
Sbjct: 30  LTWSIVKEAITQLY--STSFPTALTIADLGCSSGPNTFMVVSKLIKEVEDIRQQLHKNSI 89

Query: 67  EYQVFLNDLHGNDFNAIFTSLPSFVEDLKTQLGGGLGPCFFNGVPGSFYGRLFPTKSLHF 126
           EYQ+FLNDLHGNDFN+IF SLPSF E+LKT +G GLGPCF  G PGSFYGRLFP KSLHF
Sbjct: 90  EYQIFLNDLHGNDFNSIFKSLPSFHENLKTHMGNGLGPCFLVGAPGSFYGRLFPGKSLHF 149

Query: 127 VYSSYSLHWLSQVPKGIENNKGEIFMGSTSPKSVLEAYYKQFQKDFSMFLKSRAEELAIG 186
           V+SS SLHWLSQVP+GIENNKG IF+  TSPK+VLEAY+KQFQKDFS+FLK RAEEL  G
Sbjct: 150 VHSSNSLHWLSQVPEGIENNKGNIFISETSPKTVLEAYHKQFQKDFSLFLKCRAEELVAG 209

Query: 187 GRMVLTMIGRTSEDSSSQEYCYVWGFLSLALNNMVAEGIVGAKKVDSFNIPNYMPSPSEM 246
           GRMVLT+  RT ED  ++E CY W  L+LA+N+MV+EGIV  +K+DSFN+P YMPS  E+
Sbjct: 210 GRMVLTISARTCEDRVNKECCYSWELLNLAVNDMVSEGIVEEEKLDSFNVPIYMPSSLEV 269

Query: 247 KGEILNEGSFTIDRLEVSRINWDFYDTESHQP---NIFVDSGYYITQNIRSVIEPLLICH 306
           + E+LNEGSF I+ L+V R+NW+ Y+ E +     N  +DSGY   + IRSV EPLL+ H
Sbjct: 270 EAEVLNEGSFIIEHLQVVRVNWNIYNNELNSDIPSNEIIDSGYNYAKCIRSVAEPLLVRH 329

Query: 307 FGETIIEELFYRYRKIIDNHVSKNKIEFVNLTVSL 338
           FGE II+ELF RYR+II++H+SK  IE ++ T+SL
Sbjct: 330 FGEDIIDELFSRYREIINDHMSKENIENISFTISL 362

BLAST of MC01g1128 vs. ExPASy TrEMBL
Match: A0A5B7B8V2 (Putative Salicylate O-methyltransferase OS=Davidia involucrata OX=16924 GN=Din_034861 PE=3 SV=1)

HSP 1 Score: 437 bits (1124), Expect = 1.21e-150
Identity = 208/338 (61.54%), Postives = 266/338 (78.70%), Query Frame = 0

Query: 1   QQKVISMTRPIVKEAITNFFCRSTNFPTTLTTADLGCSSGSNTLMFVSDLIKQVEDIRHK 60
           QQKVISMT+PI ++AI + +C++   P +L  ADLGCSSG NTL+ VS+LIK VE  R K
Sbjct: 25  QQKVISMTKPITEQAIVDLYCKTV--PRSLCIADLGCSSGPNTLLVVSELIKTVEGTRKK 84

Query: 61  FHKKPLEYQVFLNDLHGNDFNAIFTSLPSFVEDLKTQLGGGLGPCFFNGVPGSFYGRLFP 120
              + LE+QV+LNDL GNDFN IF SLPSF E ++ QLG G GPCF  GVPGSFYGRLFP
Sbjct: 85  LGHQSLEFQVYLNDLPGNDFNTIFRSLPSFQEKMRKQLGSGFGPCFVTGVPGSFYGRLFP 144

Query: 121 TKSLHFVYSSYSLHWLSQVPKGIENNKGEIFMGSTSPKSVLEAYYKQFQKDFSMFLKSRA 180
           TKSLHF++SSYSL WLS+VP+G+ENNKG I+M STSP SVL+AYY+QFQ+DFS+FLK R+
Sbjct: 145 TKSLHFLHSSYSLQWLSKVPEGVENNKGNIYMASTSPPSVLKAYYEQFQRDFSVFLKCRS 204

Query: 181 EELAIGGRMVLTMIGRTSEDSSSQEYCYVWGFLSLALNNMVAEGIVGAKKVDSFNIPNYM 240
           EEL  GGRMVLT++GR SED SS+E CY+W  L++ALN MV+EGI+  +K+DSFNIP Y 
Sbjct: 205 EELVAGGRMVLTILGRKSEDPSSKECCYIWELLAMALNEMVSEGIIEEEKMDSFNIPQYT 264

Query: 241 PSPSEMKGEILNEGSFTIDRLEVSRINWDFYDTESHQPNIFVDSGYYITQNIRSVIEPLL 300
           PSP E+K E+ NEGSF +DRLEV+ +NW+ Y  E    + F D GY + + +R+V EPLL
Sbjct: 265 PSPIEVKSEVQNEGSFAVDRLEVTEVNWNAYANEFCPSDAFKDGGYNVAKCMRAVAEPLL 324

Query: 301 ICHFGETIIEELFYRYRKIIDNHVSKNKIEFVNLTVSL 338
           + HFGE II+++F+RYR+I+ + +SK + EFVN+ VSL
Sbjct: 325 VSHFGEDIIDQVFHRYREIVASRISKERTEFVNVVVSL 360

BLAST of MC01g1128 vs. ExPASy TrEMBL
Match: A0A6J1BY84 (salicylate carboxymethyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111005817 PE=4 SV=1)

HSP 1 Score: 436 bits (1121), Expect = 3.45e-150
Identity = 219/341 (64.22%), Postives = 269/341 (78.89%), Query Frame = 0

Query: 1   QQKVISMTRPIVKEAITNFFCRSTNFPTTLTTADLGCSSGSNTLMFVSDLIKQVEDIRHK 60
           QQK+ISMT  I KEAIT  +  ST+FPT +  ADLGCSSG NT M VS+LIK+VE IR +
Sbjct: 23  QQKIISMTWFITKEAITQLY--STSFPTAIAIADLGCSSGPNTFMVVSNLIKEVEKIRQQ 82

Query: 61  FHKKPLEYQVFLNDLHGNDFNAIFTSLPSFVEDLKTQLGGGLGPCFFNGVPGSFYGRLFP 120
            HK  +EYQ+FLNDLH NDFN+IF SLPSF E+LKT +G GLGPCF  G PGSFYGRLFP
Sbjct: 83  LHKNSIEYQIFLNDLHANDFNSIFKSLPSFHENLKTDMGNGLGPCFLVGAPGSFYGRLFP 142

Query: 121 TKSLHFVYSSYSLHWLSQVPKGIENNKGEIFMGSTSPKSVLEAYYKQFQKDFSMFLKSRA 180
            KSLHFV+SSYSLHWLSQVP+GIE+NKG IF+  TSPK+V+EAY+KQFQKDFS FLK RA
Sbjct: 143 GKSLHFVHSSYSLHWLSQVPEGIESNKGNIFISETSPKTVVEAYHKQFQKDFSTFLKCRA 202

Query: 181 EELAIGGRMVLTMIGRTSEDSSSQEYCYVWGFLSLALNNMVAEGIVGAKKVDSFNIPNYM 240
           EEL  GGRMV+T   R  E+  ++E CY W FL+LALN++V+EGIVG +K+DSFN+P YM
Sbjct: 203 EELVGGGRMVVTSSARICEERVNKECCYAWEFLNLALNDIVSEGIVGEEKLDSFNVPIYM 262

Query: 241 PSPSEMKGEILNEGSFTIDRLEVSRINWDFYDTESHQPNI--FVDSGYYITQNIRSVIEP 300
           PS SE++ E++NEGSF I+ L++SRINW+FY+TE +   +   +DSG    + IRSV EP
Sbjct: 263 PSLSEVEAEVMNEGSFIIEYLQISRINWNFYNTELNDIPLKGMIDSGNNFAKCIRSVAEP 322

Query: 301 LLICHFGETIIEELFYRYRKIIDNHVSK-NKIEFVNLTVSL 338
           LL  HFGE I++ELF RYR+II +H+SK N IE +N T+SL
Sbjct: 323 LLARHFGEGIMDELFSRYREIISHHMSKENNIENINFTLSL 361

BLAST of MC01g1128 vs. TAIR 10
Match: AT1G19640.1 (jasmonic acid carboxyl methyltransferase )

HSP 1 Score: 270.0 bits (689), Expect = 2.6e-72
Identity = 154/362 (42.54%), Postives = 217/362 (59.94%), Query Frame = 0

Query: 1   QQKVISMTRPIVKEAITNFFCRSTNFPTTLTTADLGCSSGSNTLMFVSDLIKQVEDIRHK 60
           Q  +IS+ R ++ EA+      ++   +++  ADLGCSSG N+L+ +S+++  + ++   
Sbjct: 25  QSNIISLGRRVMDEALKKLMMSNSEI-SSIGIADLGCSSGPNSLLSISNIVDTIHNLCPD 84

Query: 61  FHKKPLEYQVFLNDLHGNDFNAIFTSLPSF---VEDLKTQLGGGLG---PCFFNGVPGSF 120
             +   E +V LNDL  NDFN I  SLP F   V + K  LG G G    CF + VPGSF
Sbjct: 85  LDRPVPELRVSLNDLPSNDFNYICASLPEFYDRVNNNKEGLGFGRGGGESCFVSAVPGSF 144

Query: 121 YGRLFPTKSLHFVYSSYSLHWLSQVP-----------KGIENNKGEIFMGSTSPKSVLEA 180
           YGRLFP +SLHFV+SS SLHWLSQVP                N G+I++  TSPKS  +A
Sbjct: 145 YGRLFPRRSLHFVHSSSSLHWLSQVPCREAEKEDRTITADLENMGKIYISKTSPKSAHKA 204

Query: 181 YYKQFQKDFSMFLKSRAEELAIGGRMVLTMIGRTSEDSSSQEYCYVWGFLSLALNNMVAE 240
           Y  QFQ DF +FL+SR+EEL  GGRMVL+ +GR S D +++E CY W  L+ AL +M  E
Sbjct: 205 YALQFQTDFWVFLRSRSEELVPGGRMVLSFLGRRSLDPTTEESCYQWELLAQALMSMAKE 264

Query: 241 GIVGAKKVDSFNIPNYMPSPSEMKGEILNEGSFTIDRLEVSRINW-------DFYDTESH 300
           GI+  +K+D+FN P Y  S  E+K  I  EGSF+IDRLE+S I+W       + YD    
Sbjct: 265 GIIEEEKIDAFNAPYYAASSEELKMVIEKEGSFSIDRLEISPIDWEGGSISEESYDLVIR 324

Query: 301 QPNIFVDSGYYITQNIRSVIEPLLICHFGETIIEELFYRYRKIIDNHVSKNKIEFVNLTV 339
                + SG  ++  IR+V+EP+L   FGE +++ELF RY KI+  +   +   +  + +
Sbjct: 325 SKPEALASGRRVSNTIRAVVEPMLEPTFGENVMDELFERYAKIVGEYFYVSSPRYAIVIL 384

BLAST of MC01g1128 vs. TAIR 10
Match: AT3G11480.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 259.2 bits (661), Expect = 4.5e-69
Identity = 142/339 (41.89%), Postives = 201/339 (59.29%), Query Frame = 0

Query: 1   QQKVISMTRPIVKEAITNFFCRSTNFPTTLTTADLGCSSGSNTLMFVSDLIKQVEDIRHK 60
           Q+KV+SM +P++    T     + +FPT +  A+LGCSSG N+ + + ++I  +  +   
Sbjct: 47  QKKVLSMAKPVLVRN-TEEMMMNLDFPTYIKVAELGCSSGQNSFLAIFEIINTINVLCQH 106

Query: 61  FHKKPLEYQVFLNDLHGNDFNAIFTSLPSFVEDLKTQLGGGLGPCFFNGVPGSFYGRLFP 120
            +K   E    LNDL  NDFN  F  +P F ++L          CF  G PGSFY RLF 
Sbjct: 107 VNKNSPEIDCCLNDLPENDFNTTFKFVPFFNKELMIT---NKSSCFVYGAPGSFYSRLFS 166

Query: 121 TKSLHFVYSSYSLHWLSQVPKGIENNKGEIFMGSTSPKSVLEAYYKQFQKDFSMFLKSRA 180
             SLH ++SSY+LHWLS+VP+ +ENNKG +++ S+SP+S  +AY  QFQKDF+MFL+ R+
Sbjct: 167 RNSLHLIHSSYALHWLSKVPEKLENNKGNLYITSSSPQSAYKAYLNQFQKDFTMFLRLRS 226

Query: 181 EELAIGGRMVLTMIGR-TSEDSSSQEYCYVWGFLSLALNNMVAEGIVGAKKVDSFNIPNY 240
           EE+   GRMVLT IGR T  D   ++ C+ W  LS +L ++V EG+V   K+D+FN+P Y
Sbjct: 227 EEIVSNGRMVLTFIGRNTLNDPLYRDCCHFWTLLSNSLRDLVFEGLVSESKLDAFNMPFY 286

Query: 241 MPSPSEMKGEILNEGSFTIDRLEVSRINWDFYDTESHQPNIFVDSGYYITQNIRSVIEPL 300
            P+  E+K  I  EGSF I+ LE    +   Y  E        ++G      IR+V EP+
Sbjct: 287 DPNVQELKEVIQKEGSFEINELESHGFDLGHYYEEDD-----FEAGRNEANGIRAVSEPM 346

Query: 301 LICHFGETIIEELFYRYRKIIDNHVSKNKIEFVNLTVSL 339
           LI HFGE II+ LF +Y   +  H +      V+L VSL
Sbjct: 347 LIAHFGEEIIDTLFDKYAYHVTQHANCRNKTTVSLVVSL 376

BLAST of MC01g1128 vs. TAIR 10
Match: AT5G66430.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 258.8 bits (660), Expect = 5.9e-69
Identity = 141/341 (41.35%), Postives = 205/341 (60.12%), Query Frame = 0

Query: 1   QQKVISMTRPIVKEAITNFFCRSTNFPTTLTTADLGCSSGSNTLMFVSDLIKQVEDIRHK 60
           Q+KV+S  +P++ +  T     + NFP  +  ADLGC++G NT + +++++  +  +  +
Sbjct: 17  QKKVLSKAKPVLVKN-TKGMMINLNFPNYIKVADLGCATGENTFLTMAEIVNTINVLCQQ 76

Query: 61  FHKKPLEYQVFLNDLHGNDFNAIFTSLPSFVEDLKTQLGGGLGPCFFNGVPGSFYGRLFP 120
            ++KP E    LNDL  NDFN  F  +P F + +K++       CF +GVPGSFY RLFP
Sbjct: 77  CNQKPPEIDCCLNDLPDNDFNTTFKFVPFFNKRVKSK-----RLCFVSGVPGSFYSRLFP 136

Query: 121 TKSLHFVYSSYSLHWLSQVPKGIENNKGEIFMGSTSPKSVLEAYYKQFQKDFSMFLKSRA 180
            KSLHFV+SSYSLHWLS+VPKG+E N   +++ ++SP +  +AY  QFQ DF  FL+ R+
Sbjct: 137 RKSLHFVHSSYSLHWLSKVPKGLEKNSSSVYITTSSPPNAYKAYLNQFQSDFKSFLEMRS 196

Query: 181 EELAIGGRMVLTMIGR-TSEDSSSQEYCYVWGFLSLALNNMVAEGIVGAKKVDSFNIPNY 240
           EE+   GRMVLT IGR T +D   ++ C+ W  LS +L ++V EG+V A KVDSFNIP Y
Sbjct: 197 EEMVSNGRMVLTFIGRKTLDDPLHRDCCHFWTLLSTSLRDLVYEGLVSASKVDSFNIPFY 256

Query: 241 MPSPSEMKGEILNEGSFTIDRLEVS--RINWDFYDTESHQPNIFVDSGYYITQNIRSVIE 300
            PS  E+   I NEGSF I+ LE+    +    +D +    +    +G      IR+V E
Sbjct: 257 DPSKEEVMEMIRNEGSFEINDLEIHGFELGLSNHDEDYMLHSQISKAGQREANCIRAVSE 316

Query: 301 PLLICHFGETIIEELFYRYRKIIDNHVSKNKIEFVNLTVSL 339
            +L+  FG  I++ LF ++   +  H S      V L VSL
Sbjct: 317 SMLVADFGVDIMDTLFKKFAYHVSQHASCTNKTTVTLVVSL 351

BLAST of MC01g1128 vs. TAIR 10
Match: AT5G04380.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 254.2 bits (648), Expect = 1.5e-67
Identity = 140/343 (40.82%), Postives = 207/343 (60.35%), Query Frame = 0

Query: 1   QQKVISMTRPIV----KEAITNFFCRSTNFPTTLTTADLGCSSGSNTLMFVSDLIKQVED 60
           Q++V+S+T PI+    +E +TN      +FP  +  ADLGCSSG NT + +S+++  +  
Sbjct: 50  QKRVLSITNPILVKNTEEMLTNL-----DFPKCIKVADLGCSSGQNTFLAMSEIVNTINV 109

Query: 61  IRHKFHKKPLEYQVFLNDLHGNDFNAIFTSLPSFVEDLKTQLGGGLGPCFFNGVPGSFYG 120
           +  K+++   E    LNDL  NDFN  F  +  F + L +      G CF +GVPGSFY 
Sbjct: 110 LCQKWNQSRPEIDCCLNDLPTNDFNTTFKFITFFNKKLTSN-----GSCFVSGVPGSFYS 169

Query: 121 RLFPTKSLHFVYSSYSLHWLSQVPKGIENNKGEIFMGSTSPKSVLEAYYKQFQKDFSMFL 180
           RLFP KSLHF+YS YS+H+LS+VP G+E NK  +++ S+SP S  +AY  QF++DF+ FL
Sbjct: 170 RLFPRKSLHFIYSIYSIHFLSKVPDGLEKNKMSVYITSSSPLSEYKAYLNQFKRDFTTFL 229

Query: 181 KSRAEELAIGGRMVLTMIGRTSEDSS-SQEYCYVWGFLSLALNNMVAEGIVGAKKVDSFN 240
           + R+EE+   GRMVLT+IGR + D+   ++ C+ W  LS +L ++V EG++ A KV SF 
Sbjct: 230 RMRSEEMVHNGRMVLTLIGRNTLDNPLYRDCCHCWTLLSNSLRDLVFEGLLSASKVYSFK 289

Query: 241 IPNYMPSPSEMKGEILNEGSFTIDRLEVSRINWDFYDTESHQPNIFVDSGYYITQNIRSV 300
           +P Y P+  E+K  I NEGSF I+ LE+   +      +    +    +G      IR+V
Sbjct: 290 MPFYDPNEEEVKEIIRNEGSFQINDLEMHEFDLGHSKEKCSLQSHKAKAGQKEASCIRAV 349

Query: 301 IEPLLICHFGETIIEELFYRYRKIIDNHVSKNKIEFVNLTVSL 339
            E +L+ HFG+ II+ LF++Y   +  H S      V L VSL
Sbjct: 350 TETMLVAHFGDDIIDALFHKYAHHVSQHASCRVKTSVTLIVSL 382

BLAST of MC01g1128 vs. TAIR 10
Match: AT5G04370.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 250.0 bits (637), Expect = 2.7e-66
Identity = 141/339 (41.59%), Postives = 200/339 (59.00%), Query Frame = 0

Query: 1   QQKVISMTRPIVKEAITNFFCRSTNFPTTLTTADLGCSSGSNTLMFVSDLIKQVEDIRHK 60
           Q++V+S  +P++ +  T     + NFPT +  ADLGCSSG NT + +S++I  +     +
Sbjct: 57  QRRVLSKAKPVLVKN-TKDLMINLNFPTYIKVADLGCSSGQNTFLAMSEIINTINVFCQQ 116

Query: 61  FHKKPLEYQVFLNDLHGNDFNAIFTSLPSFVEDLKTQLGGGLGPCFFNGVPGSFYGRLFP 120
            ++ P E    LNDL  NDFN  F     F++             F  GVPGSFY RLFP
Sbjct: 117 RNQNPPEIDCCLNDLPSNDFNTTF----KFIQFFNGMNITSKESYFVYGVPGSFYSRLFP 176

Query: 121 TKSLHFVYSSYSLHWLSQVPKGIENNKGEIFMGSTSPKSVLEAYYKQFQKDFSMFLKSRA 180
            +SLHFV+SSY LHWLS+VP+G+E NK  +++ ++SP S  +AY  QFQ+DF+ FLK R+
Sbjct: 177 RRSLHFVHSSYGLHWLSKVPEGLEKNKMSVYITNSSPLSTYKAYLNQFQRDFATFLKLRS 236

Query: 181 EELAIGGRMVLTMIGRTSEDSS-SQEYCYVWGFLSLALNNMVAEGIVGAKKVDSFNIPNY 240
           EE+   GRMVLT IGR + D+   ++ C+ W  LS +L ++VAEG+V A KVDSF +P Y
Sbjct: 237 EEMVSNGRMVLTFIGRNTIDNPLHRDCCHFWTLLSKSLRDLVAEGLVSASKVDSFYLPFY 296

Query: 241 MPSPSEMKGEILNEGSFTIDRLEVSRINWDFYDTESHQPNIFVDSGYYITQNIRSVIEPL 300
            P+  E+K  +  EGSF I  LE    +    + +  + +    SG      IR+V EPL
Sbjct: 297 DPNEKEIKEMVQKEGSFEIRDLETHGYDLGHCNQDESKRS---KSGQNEANYIRAVSEPL 356

Query: 301 LICHFGETIIEELFYRYRKIIDNHVSKNKIEFVNLTVSL 339
           L  HFG+ II  LF ++   +  HVS      V++ VSL
Sbjct: 357 LAAHFGDAIINILFNKFACHVSQHVSCRNKTTVSIVVSL 387

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84UB41.8e-10254.73S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 2... [more]
A4ZDG86.7e-10254.44S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 3... [more]
Q84UB51.1e-10153.85S-adenosyl-L-methionine:benzoic acid/salicylic acid carboxyl methyltransferase 1... [more]
Q9SPV42.2e-9753.69Salicylate carboxymethyltransferase OS=Clarkia breweri OX=36903 GN=SAMT PE=1 SV=... [more]
A0A061FBW26.1e-8746.99Probable jasmonic acid carboxyl methyltransferase 2 OS=Theobroma cacao OX=3641 G... [more]
Match NameE-valueIdentityDescription
XP_022133322.12.76e-248100.00salicylate carboxymethyltransferase-like [Momordica charantia][more]
XP_022132391.14.94e-16670.47salicylate carboxymethyltransferase-like [Momordica charantia][more]
KAF3959833.11.74e-15162.72hypothetical protein CMV_015386 [Castanea mollissima][more]
XP_022133132.14.50e-15165.07salicylate carboxymethyltransferase-like [Momordica charantia][more]
XP_023918353.12.50e-15061.24salicylate carboxymethyltransferase-like [Quercus suber][more]
Match NameE-valueIdentityDescription
A0A6J1BWC51.34e-248100.00salicylate carboxymethyltransferase-like OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A6J1BS512.39e-16670.47salicylate carboxymethyltransferase-like OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A6J1BVR92.18e-15165.07salicylate carboxymethyltransferase-like OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A5B7B8V21.21e-15061.54Putative Salicylate O-methyltransferase OS=Davidia involucrata OX=16924 GN=Din_0... [more]
A0A6J1BY843.45e-15064.22salicylate carboxymethyltransferase-like OS=Momordica charantia OX=3673 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT1G19640.12.6e-7242.54jasmonic acid carboxyl methyltransferase [more]
AT3G11480.14.5e-6941.89S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G66430.15.9e-6941.35S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G04380.11.5e-6740.82S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G04370.12.7e-6641.59S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 1..328
e-value: 1.5E-127
score: 427.2
NoneNo IPR availablePANTHERPTHR31009:SF98SAM DEPENDENT CARBOXYL METHYLTRANSFERASE-RELATEDcoord: 1..338
IPR042086Methyltransferase, alpha-helical capping domainGENE3D1.10.1200.270coord: 138..327
e-value: 1.5E-127
score: 427.2
IPR005299SAM dependent carboxyl methyltransferasePFAMPF03492Methyltransf_7coord: 15..338
e-value: 6.1E-116
score: 387.3
IPR005299SAM dependent carboxyl methyltransferasePANTHERPTHR31009S-ADENOSYL-L-METHIONINE:CARBOXYL METHYLTRANSFERASE FAMILY PROTEINcoord: 1..338
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 1..338

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC01g1128.1MC01g1128.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
molecular_function GO:0046872 metal ion binding
molecular_function GO:0008757 S-adenosylmethionine-dependent methyltransferase activity
molecular_function GO:0008168 methyltransferase activity