Carg01085 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg01085
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	RING-type E3 ubiquitin transferase
Location	Carg_Chr06: 1293129 .. 1295574 (-)
RNA-Seq Expression	Carg01085
Synteny	Carg01085

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTGGGAGCAAAGAAGCGAAGACGAACGGGGACTGTGCCGAGGAAGCGAACCAGAAAGTTTCTGCCTCGCCGGTAAGCTCCTTTATGTAATGCTGAATTGAATGATTTTCAGTGTATAAATGCCCCAATAAATTAGGTTTTACGGTAAACATTTACACGTAGAATCCTTTTTTCCCGAAGTGGGATGTTTCTGTAGTTCGTTCTCAATTTTCGGATTGCTTCTTTTATGCCTTTCATCTGTTTACAAGTGTTTCTGTTCGGAACGTCGGTTTTCGATATCATATTTGCATTGTTGACAATTATCGTCACAATTTGGTTTTTTAGATTGGATTTCTGGAGAGGGAGGCTATTAGTTTCATCTTGTTTTGGGTAGAGAGGTGGAGGGGGATTCTTTAAGGGAGAAATATGCTTCAAATCCGGCTTAGCAAAGTTCCACAGGCGGAAGGTGGCGCTGGAGCAGTGAAGCCCTTGCAAGCGGAGACTGTGACGGTCGCATGCCCTGACCACCTTATCCTTGCTGATCTTCCTGTGGCAAAGGGCATTGGTGCGGCTACTGCTGCTTCCATTGTTAAGTCTGTTGGTCGCAGATCTCGCCGTCACCTTGGAGAACGAGTTCATTTTTGTGTCAGATGCGATTTTCCCATTGCAGTTTATGGTCGCCTGGTATTTTCCCCCACTATATGTTTGTGTCTTTTTTCTGTCGGTTGCTTTGTCTACTTTTGGTCCATGGAAATCATTCATTTATAAAAGACAATTTGAGATCAACTTTTCTTTTTGTCAAATCGGTATGCATTACTTCATTCATATCTGCAATGTTCTTCCTTCTTGCCCGCTGGTGTGTAGGCTTGTATCATTTCTTTTAAAATATTTGTGAGCATCTCTAGCCTGTGTGCATCTTGACTAATCTCACAGGACACCCACTTGACTCTACTACATTTGGGTTGTGGCATACTTAAAGAGCTAAATAGTGGTTTGCCATTCTTGCAGAGCCCATGCGAGCATGTGTTCTGTCTCGATTGTGCAAGAAGCGATTCTATATGCTACCTGTAAGTAAAAATGCTTTCAAGGTCTTAAAATGGCTATTATGGTTCTTTTGTACTATCAATGTCAATATTGATTGCCCATGCATAATCACTTTCTATTGCAGAAACTTTTCTTGTCATGTTCTTCAAAAATCTGTTGTTTTCTTTGATGATTAGAGTACTCTATACATTAGCTTCTGTGTTCTTCATGATAATATAATGTAGACGACTTTAGAAGTTGTGATTATAGTCATATTTTTTATGACACAGTTGTGATGACCGTATCCAGAAGATTCAAACTATAAAATTGATGGAGGGGATCTTCATTTGTGCAGCCCCACATTGTCTCAAGTCTTTTCTTAAGAGGACTGAATTTGAATCTCATATACAAGAGAGTCACGCTGACCTTCTTAAGCCAAATGCAGAGAAGGAAGATGGAAACGAAATTGAGGCTCTTAGTGCCAAACAATCTACAGCTTCTGAATCCACTGTCCGAGGTCTGTCGAGACCAATCATATCTCCAAGTTCCAATTCCCAGGCTCAAGAACGTGACGAAAAATTTCATCGTCAACAATCTAGAGACCAACCCACGTCTGGAATGCAGCAACAAGACAGCGGTGGTCATTCACAAGGCTTTGATCGACATGGCCCTCATGGCCGTTTTCCCCCGCAAAACTTTGACGTACAGGGTACTCCACATCAGGAATCCAGTCAATTTTCAGAGAAACAGCAGGGAATTCTGGCTGACCCTCCATACTCCCAGTACCCTCCTTTGCAATCAATTCCGCCACCAAATTACGTGGTTCCAGCTAACTCAAATCCAATGTTGAGTCCCCCTCTACCGTTCGGTTTTCCTCCGTTTCCAACTGAGGGAGCACAACAATATTACAGTACACCCTATGAAGTGCCACGACAAGATACCGCAGCAGAGACTGGATCCGAGCAAGGGTCACTGCTAGGCTTTCCACCAGGAGCAGCAGGAGGAATGAACTTCTCGGCGACTTATTCTCAACCTTGGAATACTGGACAAGCTGGCGTTCCTTATGAGCATGCTGCTGGAGGTCAAGGAATGCCTGATGCTTTTGCTAATGCACCTGATTCTCAAGTAAATCATGGATTTTACCAAGGAGATTATAGACGCAGCCCTGGAGGCTTGCCTGTGAATTCCTCTGTCTCGTCAATGGCCAACAAGAGCATGGACCTGGGGCATTCTGGTAATGCCATGGACTTGAAGAATGGTAGAGGTATATTGGCACCACAGCCACCTCTAGCACAACTTCCTCTGCCTCCGCCACTCCCACCGTACTTGTCGCATAATAAGCGTGGTAAGTTTCATTCAGGAGATACGGATCATGACGGGCAAGGCCGTGGATGGCAGAATGATAGCCATAGCCGAGATAGCTTTGGAAGTGGCCAAGACTGA

mRNA sequence

ATTTGGGAGCAAAGAAGCGAAGACGAACGGGGACTGTGCCGAGGAAGCGAACCAGAAAGTTTCTGCCTCGCCGGTAAGCTCCTTTATGTAATGCTGAATTGAATGATTTTCAGTGTATAAATGCCCCAATAAATTAGGTTTTACGGTAAACATTTACACTGGGATGTTTCTGTAGTTCGTTCTCAATTTTCGGATTGCTTCTTTTATGCCTTTCATCTGTTTACAAGTGTTTCTGTTCGTTGACAATTATCGTCACAATTTGGTTTTTTAGATTGGATTTCTGGAGAGGGAGGCTATTAGTTTCATCTTGTTTTGGGTAGAGAGGTGGAGGGGGATTCTTTAAGGGAGAAATATGCTTCAAATCCGGCTTAGCAAAGTTCCACAGGCGGAAGGTGGCGCTGGAGCAGTGAAGCCCTTGCAAGCGGAGACTGTGACGGTCGCATGCCCTGACCACCTTATCCTTGCTGATCTTCCTGTGGCAAAGGGCATTGGTGCGGCTACTGCTGCTTCCATTGTTAAGTCTGTTGGTCGCAGATCTCGCCGTCACCTTGGAGAACGAGTTCATTTTTGTGTCAGATGCGATTTTCCCATTGCAGTTTATGGTCGCCTGAAGATTCAAACTATAAAATTGATGGAGGGGATCTTCATTTGTGCAGCCCCACATTGTCTCAAGTCTTTTCTTAAGAGGACTGAATTTGAATCTCATATACAAGAGAGTCACGCTGACCTTCTTAAGCCAAATGCAGAGAAGGAAGATGGAAACGAAATTGAGGCTCTTAGTGCCAAACAATCTACAGCTTCTGAATCCACTGTCCGAGGTCTGTCGAGACCAATCATATCTCCAAGTTCCAATTCCCAGGCTCAAGAACGTGACGAAAAATTTCATCGTCAACAATCTAGAGACCAACCCACGTCTGGAATGCAGCAACAAGACAGCGGTGGTCATTCACAAGGCTTTGATCGACATGGCCCTCATGGCCGTTTTCCCCCGCAAAACTTTGACGTACAGGGTACTCCACATCAGGAATCCAGTCAATTTTCAGAGAAACAGCAGGGAATTCTGGCTGACCCTCCATACTCCCAGTACCCTCCTTTGCAATCAATTCCGCCACCAAATTACGTGGTTCCAGCTAACTCAAATCCAATGTTGAGTCCCCCTCTACCGTTCGGTTTTCCTCCGTTTCCAACTGAGGGAGCACAACAATATTACAGTACACCCTATGAAGTGCCACGACAAGATACCGCAGCAGAGACTGGATCCGAGCAAGGGTCACTGCTAGGCTTTCCACCAGGAGCAGCAGGAGGAATGAACTTCTCGGCGACTTATTCTCAACCTTGGAATACTGGACAAGCTGGCGTTCCTTATGAGCATGCTGCTGGAGGTCAAGGAATGCCTGATGCTTTTGCTAATGCACCTGATTCTCAAGTAAATCATGGATTTTACCAAGGAGATTATAGACGCAGCCCTGGAGGCTTGCCTGTGAATTCCTCTGTCTCGTCAATGGCCAACAAGAGCATGGACCTGGGGCATTCTGGTAATGCCATGGACTTGAAGAATGGTAGAGGTATATTGGCACCACAGCCACCTCTAGCACAACTTCCTCTGCCTCCGCCACTCCCACCGTACTTGTCGCATAATAAGCGTGGTAAGTTTCATTCAGGAGATACGGATCATGACGGGCAAGGCCGTGGATGGCAGAATGATAGCCATAGCCGAGATAGCTTTGGAAGTGGCCAAGACTGA

Coding sequence (CDS)

ATGCTTCAAATCCGGCTTAGCAAAGTTCCACAGGCGGAAGGTGGCGCTGGAGCAGTGAAGCCCTTGCAAGCGGAGACTGTGACGGTCGCATGCCCTGACCACCTTATCCTTGCTGATCTTCCTGTGGCAAAGGGCATTGGTGCGGCTACTGCTGCTTCCATTGTTAAGTCTGTTGGTCGCAGATCTCGCCGTCACCTTGGAGAACGAGTTCATTTTTGTGTCAGATGCGATTTTCCCATTGCAGTTTATGGTCGCCTGAAGATTCAAACTATAAAATTGATGGAGGGGATCTTCATTTGTGCAGCCCCACATTGTCTCAAGTCTTTTCTTAAGAGGACTGAATTTGAATCTCATATACAAGAGAGTCACGCTGACCTTCTTAAGCCAAATGCAGAGAAGGAAGATGGAAACGAAATTGAGGCTCTTAGTGCCAAACAATCTACAGCTTCTGAATCCACTGTCCGAGGTCTGTCGAGACCAATCATATCTCCAAGTTCCAATTCCCAGGCTCAAGAACGTGACGAAAAATTTCATCGTCAACAATCTAGAGACCAACCCACGTCTGGAATGCAGCAACAAGACAGCGGTGGTCATTCACAAGGCTTTGATCGACATGGCCCTCATGGCCGTTTTCCCCCGCAAAACTTTGACGTACAGGGTACTCCACATCAGGAATCCAGTCAATTTTCAGAGAAACAGCAGGGAATTCTGGCTGACCCTCCATACTCCCAGTACCCTCCTTTGCAATCAATTCCGCCACCAAATTACGTGGTTCCAGCTAACTCAAATCCAATGTTGAGTCCCCCTCTACCGTTCGGTTTTCCTCCGTTTCCAACTGAGGGAGCACAACAATATTACAGTACACCCTATGAAGTGCCACGACAAGATACCGCAGCAGAGACTGGATCCGAGCAAGGGTCACTGCTAGGCTTTCCACCAGGAGCAGCAGGAGGAATGAACTTCTCGGCGACTTATTCTCAACCTTGGAATACTGGACAAGCTGGCGTTCCTTATGAGCATGCTGCTGGAGGTCAAGGAATGCCTGATGCTTTTGCTAATGCACCTGATTCTCAAGTAAATCATGGATTTTACCAAGGAGATTATAGACGCAGCCCTGGAGGCTTGCCTGTGAATTCCTCTGTCTCGTCAATGGCCAACAAGAGCATGGACCTGGGGCATTCTGGTAATGCCATGGACTTGAAGAATGGTAGAGGTATATTGGCACCACAGCCACCTCTAGCACAACTTCCTCTGCCTCCGCCACTCCCACCGTACTTGTCGCATAATAAGCGTGGTAAGTTTCATTCAGGAGATACGGATCATGACGGGCAAGGCCGTGGATGGCAGAATGATAGCCATAGCCGAGATAGCTTTGGAAGTGGCCAAGACTGA

Protein sequence

MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGRRSRRHLGERVHFCVRCDFPIAVYGRLKIQTIKLMEGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQSTASESTVRGLSRPIISPSSNSQAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPHGRFPPQNFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSPPLPFGFPPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYSQPWNTGQAGVPYEHAAGGQGMPDAFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKSMDLGHSGNAMDLKNGRGILAPQPPLAQLPLPPPLPPYLSHNKRGKFHSGDTDHDGQGRGWQNDSHSRDSFGSGQD

Homology

[Close]

BLAST of Carg01085 vs. NCBI nr
Match: KAG7027916.1 (hypothetical protein SDJN02_09095 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 919.8 bits (2376), Expect = 9.1e-264
Identity = 463/463 (100.00%), Postives = 463/463 (100.00%), Query Frame = 0


Query: 1   MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGR 60
           MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRHLGERVHFCVRCDFPIAVYGRLKIQTIKLMEGIFICAAPHCLKSFLKRTEFESHIQ 120
           RSRRHLGERVHFCVRCDFPIAVYGRLKIQTIKLMEGIFICAAPHCLKSFLKRTEFESHIQ
Sbjct: 61  RSRRHLGERVHFCVRCDFPIAVYGRLKIQTIKLMEGIFICAAPHCLKSFLKRTEFESHIQ 120

Query: 121 ESHADLLKPNAEKEDGNEIEALSAKQSTASESTVRGLSRPIISPSSNSQAQERDEKFHRQ 180
           ESHADLLKPNAEKEDGNEIEALSAKQSTASESTVRGLSRPIISPSSNSQAQERDEKFHRQ
Sbjct: 121 ESHADLLKPNAEKEDGNEIEALSAKQSTASESTVRGLSRPIISPSSNSQAQERDEKFHRQ 180

Query: 181 QSRDQPTSGMQQQDSGGHSQGFDRHGPHGRFPPQNFDVQGTPHQESSQFSEKQQGILADP 240
           QSRDQPTSGMQQQDSGGHSQGFDRHGPHGRFPPQNFDVQGTPHQESSQFSEKQQGILADP
Sbjct: 181 QSRDQPTSGMQQQDSGGHSQGFDRHGPHGRFPPQNFDVQGTPHQESSQFSEKQQGILADP 240

Query: 241 PYSQYPPLQSIPPPNYVVPANSNPMLSPPLPFGFPPFPTEGAQQYYSTPYEVPRQDTAAE 300
           PYSQYPPLQSIPPPNYVVPANSNPMLSPPLPFGFPPFPTEGAQQYYSTPYEVPRQDTAAE
Sbjct: 241 PYSQYPPLQSIPPPNYVVPANSNPMLSPPLPFGFPPFPTEGAQQYYSTPYEVPRQDTAAE 300

Query: 301 TGSEQGSLLGFPPGAAGGMNFSATYSQPWNTGQAGVPYEHAAGGQGMPDAFANAPDSQVN 360
           TGSEQGSLLGFPPGAAGGMNFSATYSQPWNTGQAGVPYEHAAGGQGMPDAFANAPDSQVN
Sbjct: 301 TGSEQGSLLGFPPGAAGGMNFSATYSQPWNTGQAGVPYEHAAGGQGMPDAFANAPDSQVN 360

Query: 361 HGFYQGDYRRSPGGLPVNSSVSSMANKSMDLGHSGNAMDLKNGRGILAPQPPLAQLPLPP 420
           HGFYQGDYRRSPGGLPVNSSVSSMANKSMDLGHSGNAMDLKNGRGILAPQPPLAQLPLPP
Sbjct: 361 HGFYQGDYRRSPGGLPVNSSVSSMANKSMDLGHSGNAMDLKNGRGILAPQPPLAQLPLPP 420

Query: 421 PLPPYLSHNKRGKFHSGDTDHDGQGRGWQNDSHSRDSFGSGQD 464
           PLPPYLSHNKRGKFHSGDTDHDGQGRGWQNDSHSRDSFGSGQD
Sbjct: 421 PLPPYLSHNKRGKFHSGDTDHDGQGRGWQNDSHSRDSFGSGQD 463

[Close]

BLAST of Carg01085 vs. NCBI nr
Match: KAG6596366.1 (E3 ubiquitin-protein ligase HAKAI-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 905.6 bits (2339), Expect = 1.8e-259
Identity = 463/489 (94.68%), Postives = 463/489 (94.68%), Query Frame = 0


Query: 1   MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGR 60
           MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRHLGERVHFCVRCDFPIAVYGRL--------------------------KIQTIKLM 120
           RSRRHLGERVHFCVRCDFPIAVYGRL                          KIQTIKLM
Sbjct: 61  RSRRHLGERVHFCVRCDFPIAVYGRLSPCEHVFCLDCARSDSICYLCDDRIQKIQTIKLM 120

Query: 121 EGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQSTASESTV 180
           EGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQSTASESTV
Sbjct: 121 EGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQSTASESTV 180

Query: 181 RGLSRPIISPSSNSQAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPHGRFPPQ 240
           RGLSRPIISPSSNSQAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPHGRFPPQ
Sbjct: 181 RGLSRPIISPSSNSQAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPHGRFPPQ 240

Query: 241 NFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSPPLPFGF 300
           NFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSPPLPFGF
Sbjct: 241 NFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSPPLPFGF 300

Query: 301 PPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYSQPWNTGQA 360
           PPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYSQPWNTGQA
Sbjct: 301 PPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYSQPWNTGQA 360

Query: 361 GVPYEHAAGGQGMPDAFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKSMDLGHS 420
           GVPYEHAAGGQGMPDAFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKSMDLGHS
Sbjct: 361 GVPYEHAAGGQGMPDAFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKSMDLGHS 420

Query: 421 GNAMDLKNGRGILAPQPPLAQLPLPPPLPPYLSHNKRGKFHSGDTDHDGQGRGWQNDSHS 464
           GNAMDLKNGRGILAPQPPLAQLPLPPPLPPYLSHNKRGKFHSGDTDHDGQGRGWQNDSHS
Sbjct: 421 GNAMDLKNGRGILAPQPPLAQLPLPPPLPPYLSHNKRGKFHSGDTDHDGQGRGWQNDSHS 480

[Close]

BLAST of Carg01085 vs. NCBI nr
Match: XP_022935351.1 (E3 ubiquitin-protein ligase Hakai-like [Cucurbita moschata] >XP_022935435.1 E3 ubiquitin-protein ligase Hakai-like [Cucurbita moschata])

HSP 1 Score: 893.3 bits (2307), Expect = 9.1e-256
Identity = 458/489 (93.66%), Postives = 458/489 (93.66%), Query Frame = 0


Query: 1   MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGR 60
           MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRHLGERVHFCVRCDFPIAVYGRL--------------------------KIQTIKLM 120
           RSRRHLGERVHFCVRCDFPIAVYGRL                          KIQTIKLM
Sbjct: 61  RSRRHLGERVHFCVRCDFPIAVYGRLSPCEHVFCLDCARSDSICYLCDDRIQKIQTIKLM 120

Query: 121 EGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQSTASESTV 180
           EGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQSTASESTV
Sbjct: 121 EGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQSTASESTV 180

Query: 181 RGLSRPIISPSSNSQAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPHGRFPPQ 240
           RGLSRPIISPS NSQAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPHGRFPPQ
Sbjct: 181 RGLSRPIISPSFNSQAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPHGRFPPQ 240

Query: 241 NFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSPPLPFGF 300
           NFDVQGTPHQESSQFSEK QGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSPPLPFGF
Sbjct: 241 NFDVQGTPHQESSQFSEKPQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSPPLPFGF 300

Query: 301 PPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYSQPWNTGQA 360
           PPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATY QPWNTGQA
Sbjct: 301 PPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYPQPWNTGQA 360

Query: 361 GVPYEHAAGGQGMPDAFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKSMDLGHS 420
           GVPYEHAAGGQGMPDAFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKSMDLGHS
Sbjct: 361 GVPYEHAAGGQGMPDAFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKSMDLGHS 420

Query: 421 GNAMDLKNGRGILAPQPPLAQLPLPPPLPPYLSHNKRGKFHSGDTDHDGQGRGWQNDSHS 464
           GNAMDLKNGRGILAPQPPLAQLPLPPPLP YLSHNKRGKFHSGDTDHDGQG GWQNDSHS
Sbjct: 421 GNAMDLKNGRGILAPQPPLAQLPLPPPLPLYLSHNKRGKFHSGDTDHDGQGHGWQNDSHS 480

[Close]

BLAST of Carg01085 vs. NCBI nr
Match: XP_023539354.1 (E3 ubiquitin-protein ligase Hakai-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 884.4 bits (2284), Expect = 4.2e-253
Identity = 453/489 (92.64%), Postives = 454/489 (92.84%), Query Frame = 0


Query: 1   MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGR 60
           MLQIRLSKVPQAEGGAG VKPLQAETVTVACPDHLILADLPVA GIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAEGGAGTVKPLQAETVTVACPDHLILADLPVANGIGAATAASIVKSVGR 60

Query: 61  RSRRHLGERVHFCVRCDFPIAVYGRL--------------------------KIQTIKLM 120
           RSRRHLGERVHFCVRCDFPIAVYGRL                          KIQTIKLM
Sbjct: 61  RSRRHLGERVHFCVRCDFPIAVYGRLSPCEHVFCLDCARSDSICYLCDDRIQKIQTIKLM 120

Query: 121 EGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQSTASESTV 180
           EGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQSTASESTV
Sbjct: 121 EGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQSTASESTV 180

Query: 181 RGLSRPIISPSSNSQAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPHGRFPPQ 240
           RG SRPIISPSSNS AQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPHG FPPQ
Sbjct: 181 RGPSRPIISPSSNSLAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPHGHFPPQ 240

Query: 241 NFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSPPLPFGF 300
           NFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSPPLPFGF
Sbjct: 241 NFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSPPLPFGF 300

Query: 301 PPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYSQPWNTGQA 360
           PPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATY QPWNTGQA
Sbjct: 301 PPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYPQPWNTGQA 360

Query: 361 GVPYEHAAGGQGMPDAFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKSMDLGHS 420
           GVPYEHAAGGQGMPD FANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKSMDLGHS
Sbjct: 361 GVPYEHAAGGQGMPDTFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKSMDLGHS 420

Query: 421 GNAMDLKNGRGILAPQPPLAQLPLPPPLPPYLSHNKRGKFHSGDTDHDGQGRGWQNDSHS 464
           GNAMDLKNGRGILAPQPPLAQLPLPPPLP YLSHNKRGKFHSGDTDHDGQG GWQNDSHS
Sbjct: 421 GNAMDLKNGRGILAPQPPLAQLPLPPPLPLYLSHNKRGKFHSGDTDHDGQGHGWQNDSHS 480

[Close]

BLAST of Carg01085 vs. NCBI nr
Match: XP_022971545.1 (E3 ubiquitin-protein ligase Hakai-like [Cucurbita maxima] >XP_022971546.1 E3 ubiquitin-protein ligase Hakai-like [Cucurbita maxima])

HSP 1 Score: 881.3 bits (2276), Expect = 3.6e-252
Identity = 452/489 (92.43%), Postives = 453/489 (92.64%), Query Frame = 0


Query: 1   MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGR 60
           MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRHLGERVHFCVRCDFPIAVYGRL--------------------------KIQTIKLM 120
           RSRR LGERVHFCVRCDFPIAVYGRL                          KIQTIKLM
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAVYGRLSPCEHVFCLDCARSDSICYLCDDRIQKIQTIKLM 120

Query: 121 EGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQSTASESTV 180
           EGIFICAAPHCLKSFLKRTEFESHIQESHA+LLKPNAE EDGNEIEALSAKQSTASESTV
Sbjct: 121 EGIFICAAPHCLKSFLKRTEFESHIQESHAELLKPNAEMEDGNEIEALSAKQSTASESTV 180

Query: 181 RGLSRPIISPSSNSQAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPHGRFPPQ 240
           RG SRPIISPSSNSQAQE DEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPHGRFPPQ
Sbjct: 181 RGPSRPIISPSSNSQAQEHDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPHGRFPPQ 240

Query: 241 NFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSPPLPFGF 300
           NFDVQG PHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSPPLPFGF
Sbjct: 241 NFDVQGPPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSPPLPFGF 300

Query: 301 PPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYSQPWNTGQA 360
           PPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATY QPWNTGQA
Sbjct: 301 PPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYPQPWNTGQA 360

Query: 361 GVPYEHAAGGQGMPDAFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKSMDLGHS 420
           GVPYEHAAGGQGMPDAFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKSMDLGH 
Sbjct: 361 GVPYEHAAGGQGMPDAFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKSMDLGHY 420

Query: 421 GNAMDLKNGRGILAPQPPLAQLPLPPPLPPYLSHNKRGKFHSGDTDHDGQGRGWQNDSHS 464
           GNAMDLKNGRGILAPQPPLAQLPLPPP PPYLSHNK GKFHSGDTDHDGQG GWQNDSHS
Sbjct: 421 GNAMDLKNGRGILAPQPPLAQLPLPPPRPPYLSHNKHGKFHSGDTDHDGQGHGWQNDSHS 480

[Close]

BLAST of Carg01085 vs. ExPASy Swiss-Prot
Match: Q9LFC0 (E3 ubiquitin-protein ligase HAKAI homolog OS=Arabidopsis thaliana OX=3702 GN=HAKAI PE=1 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 6.7e-60
Identity = 161/367 (43.87%), Postives = 201/367 (54.77%), Query Frame = 0


Query: 1   MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGR 60
           MLQIRL +    E G GA +P   ETVTVACPDHL+LADLPVAKGIG+ T  +++K VGR
Sbjct: 1   MLQIRLRRDSPTETGNGA-RPSPTETVTVACPDHLVLADLPVAKGIGSVTPTTVIKPVGR 60

Query: 61  RSRRHLGERVHFCVRCDFPIAVYGRL--------------------------KIQTIKLM 120
           RSRR LGERVHFCVRCDFPIA+YGRL                          KIQTIK+M
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLIPCDHAFCLECARSDSICYLCDERIQKIQTIKMM 120

Query: 121 EGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSA-KQSTASEST 180
           EGIFICAAPHCL+SFLK+ +FE+H+ + H  LL+ +AEKEDGN+ +  S  +QS+ASEST
Sbjct: 121 EGIFICAAPHCLRSFLKKLDFEAHVHDLHGSLLQADAEKEDGNQSDVQSTMQQSSASEST 180

Query: 181 VRGLSRPIISPSSNSQAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPHGRFPP 240
           +R    P+ S    S+   R   F + QS                  GF+   P    P 
Sbjct: 181 LRA---PLRSQLQQSRELNRSASFAKSQSGFSQVQNYPPDSDNSRPPGFETASPK---PG 240

Query: 241 QNFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSPPLPFG 300
             F                       P Y Q  P+  + PP+  VP N NP L  P  FG
Sbjct: 241 IRF-----------------------PDYPQ--PMNLMQPPSLPVPMNQNPGL--PQQFG 300

Query: 301 FPPFPTE---GAQQYYS-TPYEVPRQDTAAETGSEQGSLLGF-PPGAAGGMNFSATYSQP 335
           FP +PT     +QQ+++   YE+ R ++    GSEQ SLLG+ PP     +NF  +Y  P
Sbjct: 301 FPSYPTTESGSSQQFFNGAQYEMTRTESG---GSEQSSLLGYPPPSPMTNLNFQGSYPPP 330

[Close]

BLAST of Carg01085 vs. ExPASy TrEMBL
Match: A0A6J1FAM2 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111442234 PE=4 SV=1)

HSP 1 Score: 893.3 bits (2307), Expect = 4.4e-256
Identity = 458/489 (93.66%), Postives = 458/489 (93.66%), Query Frame = 0


Query: 1   MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGR 60
           MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRHLGERVHFCVRCDFPIAVYGRL--------------------------KIQTIKLM 120
           RSRRHLGERVHFCVRCDFPIAVYGRL                          KIQTIKLM
Sbjct: 61  RSRRHLGERVHFCVRCDFPIAVYGRLSPCEHVFCLDCARSDSICYLCDDRIQKIQTIKLM 120

Query: 121 EGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQSTASESTV 180
           EGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQSTASESTV
Sbjct: 121 EGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQSTASESTV 180

Query: 181 RGLSRPIISPSSNSQAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPHGRFPPQ 240
           RGLSRPIISPS NSQAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPHGRFPPQ
Sbjct: 181 RGLSRPIISPSFNSQAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPHGRFPPQ 240

Query: 241 NFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSPPLPFGF 300
           NFDVQGTPHQESSQFSEK QGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSPPLPFGF
Sbjct: 241 NFDVQGTPHQESSQFSEKPQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSPPLPFGF 300

Query: 301 PPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYSQPWNTGQA 360
           PPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATY QPWNTGQA
Sbjct: 301 PPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYPQPWNTGQA 360

Query: 361 GVPYEHAAGGQGMPDAFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKSMDLGHS 420
           GVPYEHAAGGQGMPDAFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKSMDLGHS
Sbjct: 361 GVPYEHAAGGQGMPDAFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKSMDLGHS 420

Query: 421 GNAMDLKNGRGILAPQPPLAQLPLPPPLPPYLSHNKRGKFHSGDTDHDGQGRGWQNDSHS 464
           GNAMDLKNGRGILAPQPPLAQLPLPPPLP YLSHNKRGKFHSGDTDHDGQG GWQNDSHS
Sbjct: 421 GNAMDLKNGRGILAPQPPLAQLPLPPPLPLYLSHNKRGKFHSGDTDHDGQGHGWQNDSHS 480

[Close]

BLAST of Carg01085 vs. ExPASy TrEMBL
Match: A0A6J1I614 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111470230 PE=4 SV=1)

HSP 1 Score: 881.3 bits (2276), Expect = 1.7e-252
Identity = 452/489 (92.43%), Postives = 453/489 (92.64%), Query Frame = 0


Query: 1   MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGR 60
           MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRHLGERVHFCVRCDFPIAVYGRL--------------------------KIQTIKLM 120
           RSRR LGERVHFCVRCDFPIAVYGRL                          KIQTIKLM
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAVYGRLSPCEHVFCLDCARSDSICYLCDDRIQKIQTIKLM 120

Query: 121 EGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQSTASESTV 180
           EGIFICAAPHCLKSFLKRTEFESHIQESHA+LLKPNAE EDGNEIEALSAKQSTASESTV
Sbjct: 121 EGIFICAAPHCLKSFLKRTEFESHIQESHAELLKPNAEMEDGNEIEALSAKQSTASESTV 180

Query: 181 RGLSRPIISPSSNSQAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPHGRFPPQ 240
           RG SRPIISPSSNSQAQE DEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPHGRFPPQ
Sbjct: 181 RGPSRPIISPSSNSQAQEHDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPHGRFPPQ 240

Query: 241 NFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSPPLPFGF 300
           NFDVQG PHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSPPLPFGF
Sbjct: 241 NFDVQGPPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSPPLPFGF 300

Query: 301 PPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYSQPWNTGQA 360
           PPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATY QPWNTGQA
Sbjct: 301 PPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYPQPWNTGQA 360

Query: 361 GVPYEHAAGGQGMPDAFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKSMDLGHS 420
           GVPYEHAAGGQGMPDAFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKSMDLGH 
Sbjct: 361 GVPYEHAAGGQGMPDAFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKSMDLGHY 420

Query: 421 GNAMDLKNGRGILAPQPPLAQLPLPPPLPPYLSHNKRGKFHSGDTDHDGQGRGWQNDSHS 464
           GNAMDLKNGRGILAPQPPLAQLPLPPP PPYLSHNK GKFHSGDTDHDGQG GWQNDSHS
Sbjct: 421 GNAMDLKNGRGILAPQPPLAQLPLPPPRPPYLSHNKHGKFHSGDTDHDGQGHGWQNDSHS 480

[Close]

BLAST of Carg01085 vs. ExPASy TrEMBL
Match: A0A6J1CWS1 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111015005 PE=4 SV=1)

HSP 1 Score: 772.3 bits (1993), Expect = 1.1e-219
Identity = 403/507 (79.49%), Postives = 424/507 (83.63%), Query Frame = 0


Query: 1   MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGR 60
           MLQIRLSKVPQAEGGAGAVKPL AETVTVACPDHL+LADLPVAKGIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAEGGAGAVKPLPAETVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRHLGERVHFCVRCDFPIAVYGRL--------------------------KIQTIKLM 120
           RSRR LGERVHFCVRCDFPIA+YGRL                          KIQTIKLM
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSICYLCDDRIQKIQTIKLM 120

Query: 121 EGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQSTASESTV 180
           EGIFICAAPHCLKSFLKRTEFESHI ESHADLLKPNA+KEDGNEIEA SAKQSTASEST 
Sbjct: 121 EGIFICAAPHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNEIEAHSAKQSTASESTA 180

Query: 181 RGLSRPIISPSSNSQAQERDEKFHRQQSRDQPTSGMQQ----------------QDSGGH 240
           RG SRPIISP SNSQ QER+EKFHRQQSRDQP SGMQQ                Q   GH
Sbjct: 181 RGPSRPIISPGSNSQLQEREEKFHRQQSRDQPRSGMQQKQAPNFGQNQNNPSESQPDSGH 240

Query: 241 SQGFDRHGPHGRFPPQNFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVV 300
           SQGFDRHGPHGRFPPQNFD+QG PHQES+QFS+KQQGIL D P+SQYPPLQ IPPPNYVV
Sbjct: 241 SQGFDRHGPHGRFPPQNFDLQGGPHQESNQFSDKQQGILTDTPFSQYPPLQPIPPPNYVV 300

Query: 301 PANSNPMLSPPLPFGFPPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGG 360
           P NSNPML+PPLPFG+PPFP EGAQQYYSTPYEV RQD A ++GSEQGSLLGFPPGAAGG
Sbjct: 301 PTNSNPMLTPPLPFGYPPFPIEGAQQYYSTPYEVSRQDAAVDSGSEQGSLLGFPPGAAGG 360

Query: 361 MNFSATYSQPWNTGQAGVPYEHAAGGQGMPDAFANAPDSQVNHGFYQGDYRRSPGGLPVN 420
           MNFSATY Q WNTGQAG PY+HAAG QGMPD+F+N PDSQ  HGFYQGDYRRSPGGLPVN
Sbjct: 361 MNFSATYPQSWNTGQAGAPYDHAAGSQGMPDSFSNTPDSQGKHGFYQGDYRRSPGGLPVN 420

Query: 421 SSVSSMANKSMDLGHSGNAMDLKNGRGILAPQPPLAQLPL--PPPLPPYLSHNKRGKFHS 464
           SSVSSMANK MDLGH+G AMD + G+GILAPQP + QLPL  PPP PPYLS +KRGKF+S
Sbjct: 421 SSVSSMANKGMDLGHAGTAMDFREGKGILAPQPTM-QLPLPPPPPPPPYLSQHKRGKFYS 480

[Close]

BLAST of Carg01085 vs. ExPASy TrEMBL
Match: A0A6J1J5I8 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111481547 PE=4 SV=1)

HSP 1 Score: 768.8 bits (1984), Expect = 1.3e-218
Identity = 402/507 (79.29%), Postives = 425/507 (83.83%), Query Frame = 0


Query: 1   MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGR 60
           MLQIRLSKVPQAEGGAGAVKP  AE+VTVACPDHL+LADLPVAKGIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAEGGAGAVKPSPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRHLGERVHFCVRCDFPIAVYGRL--------------------------KIQTIKLM 120
           RSRR LGERVHFCVRCDFPIA+YGRL                          KIQTIK+M
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKMM 120

Query: 121 EGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQSTASESTV 180
           EGIFICAAPHCLKSFLKRT+FESHI ESHADLLKPNAEKEDGNEIEA SAKQSTASE++V
Sbjct: 121 EGIFICAAPHCLKSFLKRTDFESHIHESHADLLKPNAEKEDGNEIEAHSAKQSTASETSV 180

Query: 181 RGLSRPIISPSSNSQAQERDEKFHRQQSRDQPTSGMQQ----------------QDSGGH 240
           R   RP+ISP SNSQ QER+EKFHRQQSRDQP SGMQQ                Q   GH
Sbjct: 181 RCPLRPLISPGSNSQPQEREEKFHRQQSRDQPRSGMQQKQAPILGQNQINTSESQQDSGH 240

Query: 241 SQGFDRHGPHGRFPPQNFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVV 300
           SQGFDRHGPHGRFPPQNFD QG PHQESSQFSEKQQGIL+D PYSQYPPLQ IPPPNYVV
Sbjct: 241 SQGFDRHGPHGRFPPQNFDAQGAPHQESSQFSEKQQGILSDTPYSQYPPLQPIPPPNYVV 300

Query: 301 PANSNPMLSPPLPFGFPPFPTEGAQQYYSTPYEVPRQDT-AAETGSEQGSLLGFPPGAAG 360
           P NSNPML+PPLPFG+PPFP EGAQQYYSTPYEVPRQDT AAETGSEQGSLLGFPPGAAG
Sbjct: 301 PPNSNPMLTPPLPFGYPPFPVEGAQQYYSTPYEVPRQDTAAAETGSEQGSLLGFPPGAAG 360

Query: 361 GMNFSATYSQPWNTGQAGVPYEHAAGGQGMPDAFANAPDSQVNHGFYQGDYRRSPGGLPV 420
           GMNFSATY  PWN GQAG+PY+HAAGGQGMPDAFAN PDSQ  HGFY GDYRRSPGG+PV
Sbjct: 361 GMNFSATYPHPWNAGQAGIPYDHAAGGQGMPDAFANTPDSQGKHGFYPGDYRRSPGGMPV 420

Query: 421 NSSVSSMANKSMDLGH-SGNAMDLKNGRGILAPQPPLAQLPLPPPLPPYLSHNKRGKFHS 464
           NS+VSSMANK MD GH SG+ MDL++G+GILAPQ PL QLP PP  PP+LSHNKRGKF+ 
Sbjct: 421 NSTVSSMANKGMDQGHASGSTMDLRDGKGILAPQ-PLIQLPPPPRPPPFLSHNKRGKFYP 480

[Close]

BLAST of Carg01085 vs. ExPASy TrEMBL
Match: A0A6J1F6T3 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441395 PE=4 SV=1)

HSP 1 Score: 766.9 bits (1979), Expect = 4.8e-218
Identity = 400/507 (78.90%), Postives = 425/507 (83.83%), Query Frame = 0


Query: 1   MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGR 60
           MLQIRLSKVPQAEGGAGAVKP  AE+VTVACPDHL+LADLPVAKGIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAEGGAGAVKPSPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRHLGERVHFCVRCDFPIAVYGRL--------------------------KIQTIKLM 120
           RSRR LGERVHFCVRCDFPIA+YGRL                          KIQTIK+M
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYLCDDRIQKIQTIKMM 120

Query: 121 EGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQSTASESTV 180
           EGIFICAAPHCLKSFLKRT+FESHI ESHADLLKPNAEKEDGNEIEA SAKQSTASE++V
Sbjct: 121 EGIFICAAPHCLKSFLKRTDFESHIHESHADLLKPNAEKEDGNEIEAHSAKQSTASETSV 180

Query: 181 RGLSRPIISPSSNSQAQERDEKFHRQQSRDQPTSGMQQ----------------QDSGGH 240
           RG  RP+ISP SNSQ QER+EKFHRQQSRDQP SGMQQ                Q   GH
Sbjct: 181 RGPLRPLISPGSNSQPQEREEKFHRQQSRDQPRSGMQQKQAPILGQNQNNASESQQDSGH 240

Query: 241 SQGFDRHGPHGRFPPQNFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVV 300
           SQGFDRHGPHGRFPPQNFD QG PHQESSQFSEKQQGIL+D PYSQYPPLQ IPPPNYVV
Sbjct: 241 SQGFDRHGPHGRFPPQNFDAQGAPHQESSQFSEKQQGILSDTPYSQYPPLQPIPPPNYVV 300

Query: 301 PANSNPMLSPPLPFGFPPFPTEGAQQYYSTPYEVPRQDT-AAETGSEQGSLLGFPPGAAG 360
           P NSNPML+PPLPFG+PPFP EGAQ+YYS+PYEVPRQDT AAETGSEQGSLLGFPPGAAG
Sbjct: 301 PPNSNPMLTPPLPFGYPPFPVEGAQKYYSSPYEVPRQDTAAAETGSEQGSLLGFPPGAAG 360

Query: 361 GMNFSATYSQPWNTGQAGVPYEHAAGGQGMPDAFANAPDSQVNHGFYQGDYRRSPGGLPV 420
           GMNFSATY  PWN GQAG+PY+HAAGGQGMPDAFAN PDSQ  HGFY GDYRRSPGG+PV
Sbjct: 361 GMNFSATYPHPWNAGQAGIPYDHAAGGQGMPDAFANTPDSQGKHGFYPGDYRRSPGGMPV 420

Query: 421 NSSVSSMANKSMDLGH-SGNAMDLKNGRGILAPQPPLAQLPLPPPLPPYLSHNKRGKFHS 464
           NS+VSSMANK MD GH SG+ MDL++G+GILAPQ PL QLP PP  PP+LSHNKRGKF+ 
Sbjct: 421 NSTVSSMANKGMDQGHASGSTMDLRDGKGILAPQ-PLIQLPPPPRPPPFLSHNKRGKFYP 480

[Close]

BLAST of Carg01085 vs. TAIR 10
Match: AT5G01160.1 (RING/U-box superfamily protein )

HSP 1 Score: 233.0 bits (593), Expect = 4.8e-61
Identity = 161/367 (43.87%), Postives = 201/367 (54.77%), Query Frame = 0


Query: 1   MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGR 60
           MLQIRL +    E G GA +P   ETVTVACPDHL+LADLPVAKGIG+ T  +++K VGR
Sbjct: 1   MLQIRLRRDSPTETGNGA-RPSPTETVTVACPDHLVLADLPVAKGIGSVTPTTVIKPVGR 60

Query: 61  RSRRHLGERVHFCVRCDFPIAVYGRL--------------------------KIQTIKLM 120
           RSRR LGERVHFCVRCDFPIA+YGRL                          KIQTIK+M
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLIPCDHAFCLECARSDSICYLCDERIQKIQTIKMM 120

Query: 121 EGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSA-KQSTASEST 180
           EGIFICAAPHCL+SFLK+ +FE+H+ + H  LL+ +AEKEDGN+ +  S  +QS+ASEST
Sbjct: 121 EGIFICAAPHCLRSFLKKLDFEAHVHDLHGSLLQADAEKEDGNQSDVQSTMQQSSASEST 180

Query: 181 VRGLSRPIISPSSNSQAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPHGRFPP 240
           +R    P+ S    S+   R   F + QS                  GF+   P    P 
Sbjct: 181 LRA---PLRSQLQQSRELNRSASFAKSQSGFSQVQNYPPDSDNSRPPGFETASPK---PG 240

Query: 241 QNFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSPPLPFG 300
             F                       P Y Q  P+  + PP+  VP N NP L  P  FG
Sbjct: 241 IRF-----------------------PDYPQ--PMNLMQPPSLPVPMNQNPGL--PQQFG 300

Query: 301 FPPFPTE---GAQQYYS-TPYEVPRQDTAAETGSEQGSLLGF-PPGAAGGMNFSATYSQP 335
           FP +PT     +QQ+++   YE+ R ++    GSEQ SLLG+ PP     +NF  +Y  P
Sbjct: 301 FPSYPTTESGSSQQFFNGAQYEMTRTESG---GSEQSSLLGYPPPSPMTNLNFQGSYPPP 330

The following BLAST results are available for this feature:

BLAST of Carg01085 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7027916.1	9.1e-264	100.00	hypothetical protein SDJN02_09095 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6596366.1	1.8e-259	94.68	E3 ubiquitin-protein ligase HAKAI-like protein, partial [Cucurbita argyrosperma ...	[more]
XP_022935351.1	9.1e-256	93.66	E3 ubiquitin-protein ligase Hakai-like [Cucurbita moschata] >XP_022935435.1 E3 u...	[more]
XP_023539354.1	4.2e-253	92.64	E3 ubiquitin-protein ligase Hakai-like [Cucurbita pepo subsp. pepo]	[more]
XP_022971545.1	3.6e-252	92.43	E3 ubiquitin-protein ligase Hakai-like [Cucurbita maxima] >XP_022971546.1 E3 ubi...	[more]

BLAST of Carg01085 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LFC0	6.7e-60	43.87	E3 ubiquitin-protein ligase HAKAI homolog OS=Arabidopsis thaliana OX=3702 GN=HAK...	[more]

BLAST of Carg01085 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FAM2	4.4e-256	93.66	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111442234...	[more]
A0A6J1I614	1.7e-252	92.43	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111470230 P...	[more]
A0A6J1CWS1	1.1e-219	79.49	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101500...	[more]
A0A6J1J5I8	1.3e-218	79.29	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111481547 P...	[more]
A0A6J1F6T3	4.8e-218	78.90	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441395...	[more]

BLAST of Carg01085 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G01160.1	4.8e-61	43.87	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 217..232
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 183..199
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 431..449
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 125..140
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 412..426
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 125..232
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 141..170
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 401..463
None	No IPR available	PANTHER	PTHR13480:SF0	E3 UBIQUITIN-PROTEIN LIGASE HAKAI	coord: 87..429
None	No IPR available	PANTHER	PTHR13480:SF0	E3 UBIQUITIN-PROTEIN LIGASE HAKAI	coord: 1..86
IPR040383	E3 ubiquitin-protein ligase HAKAI/CBLL2	PANTHER	PTHR13480	E3 UBIQUITIN-PROTEIN LIGASE HAKAI-RELATED	coord: 1..86
IPR040383	E3 ubiquitin-protein ligase HAKAI/CBLL2	PANTHER	PTHR13480	E3 UBIQUITIN-PROTEIN LIGASE HAKAI-RELATED	coord: 87..429
IPR013087	Zinc finger C2H2-type	PROSITE	PS00028	ZINC_FINGER_C2H2_1	coord: 100..123
IPR013087	Zinc finger C2H2-type	PROSITE	PS50157	ZINC_FINGER_C2H2_2	coord: 98..124 score: 8.828978

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg01085-RA	Carg01085-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0030155	regulation of cell adhesion
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0061630	ubiquitin protein ligase activity