CSPI03G17700 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G17700
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Nicastrin
Location	Chr3: 13288189 .. 13290557 (-)
RNA-Seq Expression	CSPI03G17700
Synteny	CSPI03G17700

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TATGTGGAATCAGTATAACTTTCCCGTTTTCTTAATATCTGAGAGCAGCATTTCGTCTATACAAGAAGTAATTCTGATTCTTAAACGCTTAAAACTTTAAACTCTAAGTTGTTTCAATTACTATACAACAGTTTGGCTCAGTTGCTATATGCTTCTCTCTGGAATTGAATTTTTATTTTGACTTGTCTAAATGGTACCTCTTAGATGTTCTTTAATGGTGCACATGCCTTATTTGTCATTAAAATAAGGTCATATTGTGCTTTTATAATTCTTATAAAATGTTACACAGAGGGCTTTGCATCATCTTTTTTCCTATAAAATCATCTTGTTTCTGATTTCTTGGTTTGGTTAGGGAGCTTGGTGTCCTTTTCATTTGGGTTTTGTCGTTTTTTGTTCAATAGAAAAACAATGGAGGTTGCCTCTATGTATCTTACTTGATGAGTAAGAGTTCAGTTTTAGGTTGGGAAGAAGGGGTAGTCGTGTTTGGAATCCTAATCCTCTCGAGGGTATTTCTTGTAGATCTTTTTTATGGTTGTTGTTGGATCCTTCTCCTGCTATTGAGCCGGTCTTTGATGTGCTTTGGAGGGTTAAGGTCCCTAAAAAAGTTAAGTTCTTTACTTGGGTGTGTGAACTCTCTGGATAAGCTTTTGAGGAAGAAGTGTACAGTTATGGGGCATTTCTGTTCTATTTTTTGCTGGAAGACCTGGATCACCTTCTCTAGAGTTGTCAGTTTACGTGAGTGGTGTGCAATTGTTTCTTTCAAGAGTTGATCCGAGTGCTTGGGTGACGATTGAGGAGTTTCTTCTCCATATGCCTTTCAAGGAGAAAGCTTGATCTTTATTGAGGATATCTAGAGGCAAAGAAACAATAGAGTGTAAAGGTGTGGAGAGGGACCATGGTGATGTTTGATCCTTGCTGAGGTTCCATGTTTCTCTATGGGAATCTGTTTCAAAGACGTTTTGTGATTATCCTCTAGGTAGTATCTTAGCTAGAAACCCTTTCTTTAGAGGGGTATTGTGGACTTGGTTTTTGTGGCCCTTGTATTATTTCATTTTTTTTCTCGACGAAAATAGTTGTTTTTATTAGAAAAAAAGAATCTACTGTTACAGATCATGATTCTATGTTGATTTATGTGCTCCCGTAGCTTTATTTTGCTTGATTAGATGCCCTTCTTTTAGGTTGCGTCCTTTTTTTGGGACTTTTCTTCTTTTCTTCTTCTTAATGTTTATTATTATTATTATTCTTTTAAGTGTGGTTATTGATCACAAAGCTGAAACACAGTGTGATAAGCAAAAAGTAGAAATGTTTTTGAACAAGTGCATTTTTACCAGGGTGGAAATTCATTGATGTTTTACAATTTTGTTTTCATAATGAACAAAAAGAGGATACCTTTAGTTTATATATTTTTTTAAGGTACAGTACTTATGGTGGTATAAACAAGGACATCATTTTTGATTTATGGTTGATGCTTCTTTTGTAATTGACCATAAGTTTGATCCAGTTAAATGCTTTCATGCTTAATATCAATTATGTGAATAACTATGACTAAAATAATTTATTTTGTTGCGTAAACTTACCACTTGATGCAGGCTGCTTCAAAAAATGTGAAGAGTAAGAAAGATTACATATCTAATGTTGCTGAATTTGATCTGGTGATGCAGGTACTTAGTTTTGGTGTTACATTATGATTTTGTTTCATTGATTTTATATCTTAAATGTGTTATTTATTTACTCACAGTTTGCAAGGTCTGTGTGATTGTTTTTTTCATCTTTCCCTAGACAACTAAGGCTGGAACTCATAGTTCAATGTCTTGTTTGAAAGAAGAAACATGCCTTCCATTAGGTGGATACAGGTTAGTTTTACTTGAATCTTTCTGTCTCCACATAATTTCGCACTGTTTATTTTATTAAAAGAGATCAGTATCGTTACAGTGTTTGGTCATCACTTCCCCCAATCAACACTTCTTCCTCAGATCAGTCTAAGCCTGTCATTCTCACGGTAGCTTCCATGGATTCGGCCTCTTTCTTCCGTGATAAAAGCATTGGTGCTGACTCCCCCATCTCTGTATGATTTATTTTGAGCATGTATATCAGTCATAACTCTTACCTTGTTTGTTCTGCTACCCGTTAAAGCTTTTTTTGGTACATTTTAGGGCCTGATTGCGTTGCTGGCTGCAGTTGATGCACTTTCCCATGTGGATGGGTTGGACGATCTTCATAAACAGGTTCTTATTGAAGCTGTTTTCTTTGTATATAATTGCACTTTCCTTATATTAAGAGCTTCCTAACTCAAATGGAAATATTCTATTACTTCTTTGATCTGTTTAGTTGCATTTTAGACTGAGTTAACTTTAATTCTTCCCCTGTGA

mRNA sequence

TATGTGGAATCAGTATAACTTTCCCGTTTTCTTAATATCTGAGAGCAGCATTTCGTCTATACAAGAAGCTGCTTCAAAAAATGTGAAGAGTAAGAAAGATTACATATCTAATGTTGCTGAATTTGATCTGGTGATGCAGACAACTAAGGCTGGAACTCATAGTTCAATGTCTTGTTTGAAAGAAGAAACATGCCTTCCATTAGGTGGATACAGTGTTTGGTCATCACTTCCCCCAATCAACACTTCTTCCTCAGATCAGTCTAAGCCTGTCATTCTCACGGTAGCTTCCATGGATTCGGCCTCTTTCTTCCGTGATAAAAGCATTGGTGCTGACTCCCCCATCTCTGGCCTGATTGCGTTGCTGGCTGCAGTTGATGCACTTTCCCATGTGGATGGGTTGGACGATCTTCATAAACAGACTGAGTTAACTTTAATTCTTCCCCTGTGA

Coding sequence (CDS)

ATGTGGAATCAGTATAACTTTCCCGTTTTCTTAATATCTGAGAGCAGCATTTCGTCTATACAAGAAGCTGCTTCAAAAAATGTGAAGAGTAAGAAAGATTACATATCTAATGTTGCTGAATTTGATCTGGTGATGCAGACAACTAAGGCTGGAACTCATAGTTCAATGTCTTGTTTGAAAGAAGAAACATGCCTTCCATTAGGTGGATACAGTGTTTGGTCATCACTTCCCCCAATCAACACTTCTTCCTCAGATCAGTCTAAGCCTGTCATTCTCACGGTAGCTTCCATGGATTCGGCCTCTTTCTTCCGTGATAAAAGCATTGGTGCTGACTCCCCCATCTCTGGCCTGATTGCGTTGCTGGCTGCAGTTGATGCACTTTCCCATGTGGATGGGTTGGACGATCTTCATAAACAGACTGAGTTAACTTTAATTCTTCCCCTGTGA

Protein sequence

MWNQYNFPVFLISESSISSIQEAASKNVKSKKDYISNVAEFDLVMQTTKAGTHSSMSCLKEETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQTELTLILPL*

Homology

[Close]

BLAST of CSPI03G17700 vs. ExPASy Swiss-Prot
Match: Q8GUM5 (Nicastrin OS=Arabidopsis thaliana OX=3702 GN=At3g52640/At3g52650 PE=2 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 2.2e-49
Identity = 97/139 (69.78%), Postives = 115/139 (82.73%), Query Frame = 0


Query: 1   MWNQYNFPVFLISESSISSIQEAASKNVKSKKDYISNVAEFDLVMQTTKAGTHSSMSCLK 60
           MW  YNFPV+L+SES IS++ E  SK       Y S+VAEF++VM+TTKAGTH+S +CL+
Sbjct: 163 MWRNYNFPVYLLSESGISAVHEILSKKKMKHGTYTSDVAEFNMVMETTKAGTHNSEACLQ 222

Query: 61  EETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIAL 120
           E TCLPLGGYSVWSSLPPI+ SSS+  KPV+LTVASMD+ASFFRDKS GADSPISGL+AL
Sbjct: 223 EGTCLPLGGYSVWSSLPPISVSSSNNRKPVVLTVASMDTASFFRDKSFGADSPISGLVAL 282

Query: 121 LAAVDALSHVDGLDDLHKQ 140
           L AVDALS VDG+ +L KQ
Sbjct: 283 LGAVDALSRVDGISNLKKQ 301

[Close]

BLAST of CSPI03G17700 vs. ExPASy Swiss-Prot
Match: F0ZBA6 (Nicastrin OS=Dictyostelium purpureum OX=5786 GN=ncstn PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 5.4e-11
Identity = 44/127 (34.65%), Postives = 68/127 (53.54%), Query Frame = 0


Query: 5   YNFPVFLISESSISSIQEAASKNVKSKKDYISNVAEFDLVMQTTKAGTHSSMSCLKEETC 64
           + FP+F I   +  +I+  +  N   +  Y +  AE D  MQ    G  +S +CL+   C
Sbjct: 157 FPFPIFAIDNQTSVAIRNVSKHNRDGQ--YPAWGAELDSFMQ----GAINSETCLRRGFC 216

Query: 65  LPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIALLAAV 124
            P+GG S+WSS     +S  D+ K +IL +   D+ +FFRD SIGAD      + LL+ +
Sbjct: 217 EPVGGQSIWSSF----SSKIDKEKEIILVMLPFDTTAFFRDLSIGADQSSFATVTLLSVI 273

Query: 125 DALSHVD 132
            +L+ VD
Sbjct: 277 KSLAAVD 273

[Close]

BLAST of CSPI03G17700 vs. ExPASy Swiss-Prot
Match: Q54JT7 (Nicastrin OS=Dictyostelium discoideum OX=44689 GN=ncstn PE=3 SV=2)

HSP 1 Score: 64.7 bits (156), Expect = 1.0e-09
Identity = 43/127 (33.86%), Postives = 67/127 (52.76%), Query Frame = 0


Query: 5   YNFPVFLISESSISSIQEAASKNVKSKKDYISNVAEFDLVMQTTKAGTHSSMSCLKEETC 64
           + FP+F +   +   I+  ++ N   K  Y +  AE D  MQ    G  ++ +CL+   C
Sbjct: 155 FPFPMFALELKTSIIIRNLSTINRDGK--YPAYGAELDSFMQ----GAINAETCLRRGFC 214

Query: 65  LPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIALLAAV 124
            P+GG S+WSS   +     DQSKP+IL +  +D+ +FFRD + G D     L  LL+ +
Sbjct: 215 EPVGGQSIWSSFSEV----IDQSKPIILVMLPIDATAFFRDLATGTDQSGYALTVLLSML 271

Query: 125 DALSHVD 132
           + L  VD
Sbjct: 275 NTLQGVD 271

[Close]

BLAST of CSPI03G17700 vs. ExPASy Swiss-Prot
Match: Q8CGU6 (Nicastrin OS=Rattus norvegicus OX=10116 GN=Ncstn PE=1 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 1.2e-05
Identity = 37/142 (26.06%), Postives = 70/142 (49.30%), Query Frame = 0


Query: 2   WNQYNFPVFLISESSISSIQEAASKNVKSKKDYISNVAEFDLVMQTTKAGTH---SSMSC 61
           ++ ++FP+FL+ + + + + +   ++    ++   +   F L      +  H   S+ +C
Sbjct: 172 YDDFSFPIFLLEDENETKVIKQCYQDHNLGQN--GSAPSFPLCAMQLFSHMHAVISTATC 231

Query: 62  LK------------EETCLPLGGYSVWSSLPPINTSSS-DQSKPVILTVASMDSASFFRD 121
           ++            E  C PL  Y+VWS L PINTS   +    V++    +DS SFF +
Sbjct: 232 MRRSFIQSTFSINPEIVCDPLSDYNVWSMLKPINTSGGLEPDVRVVVAATRLDSRSFFWN 291

Query: 122 KSIGADSPISGLIALLAAVDAL 128
            + GA+S ++  +  LAA +AL
Sbjct: 292 VAPGAESAVASFVTQLAAAEAL 311

[Close]

BLAST of CSPI03G17700 vs. ExPASy Swiss-Prot
Match: Q92542 (Nicastrin OS=Homo sapiens OX=9606 GN=NCSTN PE=1 SV=2)

HSP 1 Score: 50.1 bits (118), Expect = 2.6e-05
Identity = 35/140 (25.00%), Postives = 68/140 (48.57%), Query Frame = 0


Query: 2   WNQYNFPVFLISESSISSIQEAASKNVKSKKD---------YISNVAEFDLVMQTT---- 61
           +  ++FP+FL+ + + + + +   ++    ++          +   +    V+ T     
Sbjct: 173 YEDFSFPIFLLEDENETKVIKQCYQDHNLSQNGSAPTFPLCAMQLFSHMHAVISTATCMR 232

Query: 62  KAGTHSSMSCLKEETCLPLGGYSVWSSLPPINTSSS-DQSKPVILTVASMDSASFFRDKS 121
           ++   S+ S   E  C PL  Y+VWS L PINT+ +      V++    +DS SFF + +
Sbjct: 233 RSSIQSTFSINPEIVCDPLSDYNVWSMLKPINTTGTLKPDDRVVVAATRLDSRSFFWNVA 292

Query: 122 IGADSPISGLIALLAAVDAL 128
            GA+S ++  +  LAA +AL
Sbjct: 293 PGAESAVASFVTQLAAAEAL 312

[Close]

BLAST of CSPI03G17700 vs. ExPASy TrEMBL
Match: A0A0A0L8Z5 (Nicastrin OS=Cucumis sativus OX=3659 GN=Csa_3G187280 PE=3 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 3.4e-69
Identity = 139/139 (100.00%), Postives = 139/139 (100.00%), Query Frame = 0


Query: 1   MWNQYNFPVFLISESSISSIQEAASKNVKSKKDYISNVAEFDLVMQTTKAGTHSSMSCLK 60
           MWNQYNFPVFLISESSISSIQEAASKNVKSKKDYISNVAEFDLVMQTTKAGTHSSMSCLK
Sbjct: 1   MWNQYNFPVFLISESSISSIQEAASKNVKSKKDYISNVAEFDLVMQTTKAGTHSSMSCLK 60

Query: 61  EETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIAL 120
           EETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIAL
Sbjct: 61  EETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIAL 120

Query: 121 LAAVDALSHVDGLDDLHKQ 140
           LAAVDALSHVDGLDDLHKQ
Sbjct: 121 LAAVDALSHVDGLDDLHKQ 139

[Close]

BLAST of CSPI03G17700 vs. ExPASy TrEMBL
Match: A0A1S3C4C7 (Nicastrin OS=Cucumis melo OX=3656 GN=LOC103496866 PE=3 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.3e-68
Identity = 137/139 (98.56%), Postives = 139/139 (100.00%), Query Frame = 0


Query: 1   MWNQYNFPVFLISESSISSIQEAASKNVKSKKDYISNVAEFDLVMQTTKAGTHSSMSCLK 60
           MWN+YNFPVFLISESSISSIQEAASKNVKSKKDY+SNVAEFDLVMQTTKAGTHSSMSCLK
Sbjct: 172 MWNRYNFPVFLISESSISSIQEAASKNVKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLK 231

Query: 61  EETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIAL 120
           EETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIAL
Sbjct: 232 EETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIAL 291

Query: 121 LAAVDALSHVDGLDDLHKQ 140
           LAAVDALSHVDGLDDLHKQ
Sbjct: 292 LAAVDALSHVDGLDDLHKQ 310

[Close]

BLAST of CSPI03G17700 vs. ExPASy TrEMBL
Match: A0A1S3C4S1 (Nicastrin OS=Cucumis melo OX=3656 GN=LOC103496866 PE=3 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.3e-68
Identity = 137/139 (98.56%), Postives = 139/139 (100.00%), Query Frame = 0


Query: 1   MWNQYNFPVFLISESSISSIQEAASKNVKSKKDYISNVAEFDLVMQTTKAGTHSSMSCLK 60
           MWN+YNFPVFLISESSISSIQEAASKNVKSKKDY+SNVAEFDLVMQTTKAGTHSSMSCLK
Sbjct: 172 MWNRYNFPVFLISESSISSIQEAASKNVKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLK 231

Query: 61  EETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIAL 120
           EETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIAL
Sbjct: 232 EETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIAL 291

Query: 121 LAAVDALSHVDGLDDLHKQ 140
           LAAVDALSHVDGLDDLHKQ
Sbjct: 292 LAAVDALSHVDGLDDLHKQ 310

[Close]

BLAST of CSPI03G17700 vs. ExPASy TrEMBL
Match: A0A6J1K5Z2 (Nicastrin OS=Cucurbita maxima OX=3661 GN=LOC111492619 PE=3 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 2.4e-62
Identity = 128/139 (92.09%), Postives = 132/139 (94.96%), Query Frame = 0


Query: 1   MWNQYNFPVFLISESSISSIQEAASKNVKSKKDYISNVAEFDLVMQTTKAGTHSSMSCLK 60
           MWNQYNFPVFLISESSIS +QEAASKNVK KK Y SNVAEFDLVMQTTKAGTH+SMSCLK
Sbjct: 154 MWNQYNFPVFLISESSISPVQEAASKNVKDKKVYTSNVAEFDLVMQTTKAGTHNSMSCLK 213

Query: 61  EETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIAL 120
           EETCLPLGGYSVWSSLPPIN  S D+SKP+ILTVASMDSASFFRDKSIGADSPISGLIAL
Sbjct: 214 EETCLPLGGYSVWSSLPPINI-SLDKSKPIILTVASMDSASFFRDKSIGADSPISGLIAL 273

Query: 121 LAAVDALSHVDGLDDLHKQ 140
           LAAVDALSHVDGLDDLHKQ
Sbjct: 274 LAAVDALSHVDGLDDLHKQ 291

[Close]

BLAST of CSPI03G17700 vs. ExPASy TrEMBL
Match: A0A6J1CNM4 (Nicastrin OS=Momordica charantia OX=3673 GN=LOC111013258 PE=3 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 5.3e-62
Identity = 127/139 (91.37%), Postives = 133/139 (95.68%), Query Frame = 0


Query: 1   MWNQYNFPVFLISESSISSIQEAASKNVKSKKDYISNVAEFDLVMQTTKAGTHSSMSCLK 60
           MW QYNFPVFLISE+SISSI EA+SKNVK+KK Y SNVAEFDLVMQTTKAGTH+S+SCLK
Sbjct: 155 MWKQYNFPVFLISETSISSIHEASSKNVKNKKAYTSNVAEFDLVMQTTKAGTHNSVSCLK 214

Query: 61  EETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIAL 120
           EETCLPLGGYSVWSSLPPIN  SSDQSKP+ILTVASMDSASFFRDKSIGADSPISGLIAL
Sbjct: 215 EETCLPLGGYSVWSSLPPINI-SSDQSKPIILTVASMDSASFFRDKSIGADSPISGLIAL 274

Query: 121 LAAVDALSHVDGLDDLHKQ 140
           LAAVDALSHVDGLDDLHKQ
Sbjct: 275 LAAVDALSHVDGLDDLHKQ 292

[Close]

BLAST of CSPI03G17700 vs. NCBI nr
Match: XP_004140732.1 (nicastrin [Cucumis sativus] >KGN57470.2 hypothetical protein Csa_011284 [Cucumis sativus])

HSP 1 Score: 270.8 bits (691), Expect = 7.1e-69
Identity = 139/139 (100.00%), Postives = 139/139 (100.00%), Query Frame = 0


Query: 1   MWNQYNFPVFLISESSISSIQEAASKNVKSKKDYISNVAEFDLVMQTTKAGTHSSMSCLK 60
           MWNQYNFPVFLISESSISSIQEAASKNVKSKKDYISNVAEFDLVMQTTKAGTHSSMSCLK
Sbjct: 154 MWNQYNFPVFLISESSISSIQEAASKNVKSKKDYISNVAEFDLVMQTTKAGTHSSMSCLK 213

Query: 61  EETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIAL 120
           EETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIAL
Sbjct: 214 EETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIAL 273

Query: 121 LAAVDALSHVDGLDDLHKQ 140
           LAAVDALSHVDGLDDLHKQ
Sbjct: 274 LAAVDALSHVDGLDDLHKQ 292

[Close]

BLAST of CSPI03G17700 vs. NCBI nr
Match: XP_008457117.1 (PREDICTED: nicastrin isoform X2 [Cucumis melo])

HSP 1 Score: 268.9 bits (686), Expect = 2.7e-68
Identity = 137/139 (98.56%), Postives = 139/139 (100.00%), Query Frame = 0


Query: 1   MWNQYNFPVFLISESSISSIQEAASKNVKSKKDYISNVAEFDLVMQTTKAGTHSSMSCLK 60
           MWN+YNFPVFLISESSISSIQEAASKNVKSKKDY+SNVAEFDLVMQTTKAGTHSSMSCLK
Sbjct: 172 MWNRYNFPVFLISESSISSIQEAASKNVKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLK 231

Query: 61  EETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIAL 120
           EETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIAL
Sbjct: 232 EETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIAL 291

Query: 121 LAAVDALSHVDGLDDLHKQ 140
           LAAVDALSHVDGLDDLHKQ
Sbjct: 292 LAAVDALSHVDGLDDLHKQ 310

[Close]

BLAST of CSPI03G17700 vs. NCBI nr
Match: XP_008457115.1 (PREDICTED: nicastrin isoform X1 [Cucumis melo])

HSP 1 Score: 268.9 bits (686), Expect = 2.7e-68
Identity = 137/139 (98.56%), Postives = 139/139 (100.00%), Query Frame = 0


Query: 1   MWNQYNFPVFLISESSISSIQEAASKNVKSKKDYISNVAEFDLVMQTTKAGTHSSMSCLK 60
           MWN+YNFPVFLISESSISSIQEAASKNVKSKKDY+SNVAEFDLVMQTTKAGTHSSMSCLK
Sbjct: 172 MWNRYNFPVFLISESSISSIQEAASKNVKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLK 231

Query: 61  EETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIAL 120
           EETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIAL
Sbjct: 232 EETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIAL 291

Query: 121 LAAVDALSHVDGLDDLHKQ 140
           LAAVDALSHVDGLDDLHKQ
Sbjct: 292 LAAVDALSHVDGLDDLHKQ 310

[Close]

BLAST of CSPI03G17700 vs. NCBI nr
Match: XP_038896057.1 (nicastrin [Benincasa hispida])

HSP 1 Score: 264.2 bits (674), Expect = 6.7e-67
Identity = 135/139 (97.12%), Postives = 137/139 (98.56%), Query Frame = 0


Query: 1   MWNQYNFPVFLISESSISSIQEAASKNVKSKKDYISNVAEFDLVMQTTKAGTHSSMSCLK 60
           MWNQYNFPVFLISESSISSIQEAASKNVK+KKDYISNVAEFDLVMQTTKAGTH+SMSCLK
Sbjct: 154 MWNQYNFPVFLISESSISSIQEAASKNVKNKKDYISNVAEFDLVMQTTKAGTHNSMSCLK 213

Query: 61  EETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIAL 120
           E TCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIAL
Sbjct: 214 EVTCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIAL 273

Query: 121 LAAVDALSHVDGLDDLHKQ 140
           LAAVDALSHVDG DDLHKQ
Sbjct: 274 LAAVDALSHVDGFDDLHKQ 292

[Close]

BLAST of CSPI03G17700 vs. NCBI nr
Match: XP_023525268.1 (nicastrin [Cucurbita pepo subsp. pepo])

HSP 1 Score: 250.4 bits (638), Expect = 9.9e-63
Identity = 129/139 (92.81%), Postives = 133/139 (95.68%), Query Frame = 0


Query: 1   MWNQYNFPVFLISESSISSIQEAASKNVKSKKDYISNVAEFDLVMQTTKAGTHSSMSCLK 60
           MWNQYNFPVFLISESSIS +QEAASKNVK KK Y SNVAEFDLVMQTTKAGTH+SMSCLK
Sbjct: 154 MWNQYNFPVFLISESSISPVQEAASKNVKDKKVYTSNVAEFDLVMQTTKAGTHNSMSCLK 213

Query: 61  EETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIAL 120
           EETCLPLGGYSVWSSLPPIN  SSD+SKP+ILTVASMDSASFFRDKSIGADSPISGLIAL
Sbjct: 214 EETCLPLGGYSVWSSLPPINI-SSDKSKPIILTVASMDSASFFRDKSIGADSPISGLIAL 273

Query: 121 LAAVDALSHVDGLDDLHKQ 140
           LAAVDALSHVDGLDDLHKQ
Sbjct: 274 LAAVDALSHVDGLDDLHKQ 291

[Close]

BLAST of CSPI03G17700 vs. TAIR 10
Match: AT3G52640.2 (Zn-dependent exopeptidases superfamily protein )

HSP 1 Score: 196.4 bits (498), Expect = 1.6e-50
Identity = 97/139 (69.78%), Postives = 115/139 (82.73%), Query Frame = 0


Query: 1   MWNQYNFPVFLISESSISSIQEAASKNVKSKKDYISNVAEFDLVMQTTKAGTHSSMSCLK 60
           MW  YNFPV+L+SES IS++ E  SK       Y S+VAEF++VM+TTKAGTH+S +CL+
Sbjct: 163 MWRNYNFPVYLLSESGISAVHEILSKKKMKHGTYTSDVAEFNMVMETTKAGTHNSEACLQ 222

Query: 61  EETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIAL 120
           E TCLPLGGYSVWSSLPPI+ SSS+  KPV+LTVASMD+ASFFRDKS GADSPISGL+AL
Sbjct: 223 EGTCLPLGGYSVWSSLPPISVSSSNNRKPVVLTVASMDTASFFRDKSFGADSPISGLVAL 282

Query: 121 LAAVDALSHVDGLDDLHKQ 140
           L AVDALS VDG+ +L KQ
Sbjct: 283 LGAVDALSRVDGISNLKKQ 301

[Close]

BLAST of CSPI03G17700 vs. TAIR 10
Match: AT3G52640.1 (Zn-dependent exopeptidases superfamily protein )

HSP 1 Score: 196.4 bits (498), Expect = 1.6e-50
Identity = 97/139 (69.78%), Postives = 115/139 (82.73%), Query Frame = 0


Query: 1   MWNQYNFPVFLISESSISSIQEAASKNVKSKKDYISNVAEFDLVMQTTKAGTHSSMSCLK 60
           MW  YNFPV+L+SES IS++ E  SK       Y S+VAEF++VM+TTKAGTH+S +CL+
Sbjct: 163 MWRNYNFPVYLLSESGISAVHEILSKKKMKHGTYTSDVAEFNMVMETTKAGTHNSEACLQ 222

Query: 61  EETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIAL 120
           E TCLPLGGYSVWSSLPPI+ SSS+  KPV+LTVASMD+ASFFRDKS GADSPISGL+AL
Sbjct: 223 EGTCLPLGGYSVWSSLPPISVSSSNNRKPVVLTVASMDTASFFRDKSFGADSPISGLVAL 282

Query: 121 LAAVDALSHVDGLDDLHKQ 140
           L AVDALS VDG+ +L KQ
Sbjct: 283 LGAVDALSRVDGISNLKKQ 301

[Close]

BLAST of CSPI03G17700 vs. TAIR 10
Match: AT3G52640.3 (Zn-dependent exopeptidases superfamily protein )

HSP 1 Score: 196.4 bits (498), Expect = 1.6e-50
Identity = 97/139 (69.78%), Postives = 115/139 (82.73%), Query Frame = 0


Query: 1   MWNQYNFPVFLISESSISSIQEAASKNVKSKKDYISNVAEFDLVMQTTKAGTHSSMSCLK 60
           MW  YNFPV+L+SES IS++ E  SK       Y S+VAEF++VM+TTKAGTH+S +CL+
Sbjct: 163 MWRNYNFPVYLLSESGISAVHEILSKKKMKHGTYTSDVAEFNMVMETTKAGTHNSEACLQ 222

Query: 61  EETCLPLGGYSVWSSLPPINTSSSDQSKPVILTVASMDSASFFRDKSIGADSPISGLIAL 120
           E TCLPLGGYSVWSSLPPI+ SSS+  KPV+LTVASMD+ASFFRDKS GADSPISGL+AL
Sbjct: 223 EGTCLPLGGYSVWSSLPPISVSSSNNRKPVVLTVASMDTASFFRDKSFGADSPISGLVAL 282

Query: 121 LAAVDALSHVDGLDDLHKQ 140
           L AVDALS VDG+ +L KQ
Sbjct: 283 LGAVDALSRVDGISNLKKQ 301

The following BLAST results are available for this feature:

BLAST of CSPI03G17700 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GUM5	2.2e-49	69.78	Nicastrin OS=Arabidopsis thaliana OX=3702 GN=At3g52640/At3g52650 PE=2 SV=1	[more]
F0ZBA6	5.4e-11	34.65	Nicastrin OS=Dictyostelium purpureum OX=5786 GN=ncstn PE=1 SV=1	[more]
Q54JT7	1.0e-09	33.86	Nicastrin OS=Dictyostelium discoideum OX=44689 GN=ncstn PE=3 SV=2	[more]
Q8CGU6	1.2e-05	26.06	Nicastrin OS=Rattus norvegicus OX=10116 GN=Ncstn PE=1 SV=1	[more]
Q92542	2.6e-05	25.00	Nicastrin OS=Homo sapiens OX=9606 GN=NCSTN PE=1 SV=2	[more]

BLAST of CSPI03G17700 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L8Z5	3.4e-69	100.00	Nicastrin OS=Cucumis sativus OX=3659 GN=Csa_3G187280 PE=3 SV=1	[more]
A0A1S3C4C7	1.3e-68	98.56	Nicastrin OS=Cucumis melo OX=3656 GN=LOC103496866 PE=3 SV=1	[more]
A0A1S3C4S1	1.3e-68	98.56	Nicastrin OS=Cucumis melo OX=3656 GN=LOC103496866 PE=3 SV=1	[more]
A0A6J1K5Z2	2.4e-62	92.09	Nicastrin OS=Cucurbita maxima OX=3661 GN=LOC111492619 PE=3 SV=1	[more]
A0A6J1CNM4	5.3e-62	91.37	Nicastrin OS=Momordica charantia OX=3673 GN=LOC111013258 PE=3 SV=1	[more]

BLAST of CSPI03G17700 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004140732.1	7.1e-69	100.00	nicastrin [Cucumis sativus] >KGN57470.2 hypothetical protein Csa_011284 [Cucumis...	[more]
XP_008457117.1	2.7e-68	98.56	PREDICTED: nicastrin isoform X2 [Cucumis melo]	[more]
XP_008457115.1	2.7e-68	98.56	PREDICTED: nicastrin isoform X1 [Cucumis melo]	[more]
XP_038896057.1	6.7e-67	97.12	nicastrin [Benincasa hispida]	[more]
XP_023525268.1	9.9e-63	92.81	nicastrin [Cucurbita pepo subsp. pepo]	[more]

BLAST of CSPI03G17700 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G52640.2	1.6e-50	69.78	Zn-dependent exopeptidases superfamily protein	[more]
AT3G52640.1	1.6e-50	69.78	Zn-dependent exopeptidases superfamily protein	[more]
AT3G52640.3	1.6e-50	69.78	Zn-dependent exopeptidases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR041084	Nicastrin, small lobe	PFAM	PF18266	Ncstrn_small	coord: 1..47 e-value: 3.7E-7 score: 30.3
None	No IPR available	PFAM	PF05450	Nicastrin	coord: 89..139 e-value: 8.9E-13 score: 48.2
None	No IPR available	SUPERFAMILY	53187	Zn-dependent exopeptidases	coord: 62..127
IPR008710	Nicastrin	PANTHER	PTHR21092	NICASTRIN	coord: 1..139

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G17700.1	CSPI03G17700.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016485	protein processing
cellular_component	GO:0005887	integral component of plasma membrane
cellular_component	GO:0016021	integral component of membrane