CSPI01G16460 (gene) Cucumber (PI 183967) v1

BLAST of CSPI01G16460 vs. ExPASy Swiss-Prot
Match: P21616 (Pyrophosphate-energized vacuolar membrane proton pump OS=Vigna radiata var. radiata OX=3916 PE=1 SV=4)

HSP 1 Score: 1325.5 bits (3429), Expect = 0.0e+00
Identity = 695/768 (90.49%), Postives = 737/768 (95.96%), Query Frame = 0

Query: 1   MGATILPDLGAQIFIPLCAIVGILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL 60
           MGA ILPDLG +I IP+CA++GI F+L QW  VS+VKLS+ RD++ N  ++AKNGY+DYL
Sbjct: 1   MGAAILPDLGTEILIPVCAVIGIAFALFQWLLVSKVKLSAVRDASPN--AAAKNGYNDYL 60

Query: 61  IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS 120
           IEEEEG+NDHNVV+KCAEIQNAISEGATSFLFTEYKYVGIFM+ FA LIF+FLGSVEGFS
Sbjct: 61  IEEEEGINDHNVVVKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAILIFLFLGSVEGFS 120

Query: 121 TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180
           T PQ CSYDKTKTCKPALATA FST+SFLLG VTS+VSGFLGMKIATYANARTTLEARKG
Sbjct: 121 TSPQACSYDKTKTCKPALATAIFSTVSFLLGGVTSLVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGEDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVL+IAINLFK+YYG+DWGGLFE+ITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLYIAINLFKIYYGDDWGGLFEAITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFG NHE TAMLYPLIVSS+GILVCL+TTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGLNHELTAMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKEIE 360

Query: 361 PALKNQLIISTVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLII 420
           PALK QL+ISTV+MT G+A+V++V++P  FTIFNFG QK V++W+LFLCVAVGLWAGLII
Sbjct: 361 PALKKQLVISTVLMTIGVAVVSFVALPTSFTIFNFGVQKDVKSWQLFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSFSFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA SIFVSF+FAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAISIFVSFTFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRA +  VD+LTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRASITTVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPL+VGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLVVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAG SEHAR+LGPKGSD HKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARSLGPKGSDCHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF 769
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA+HGG+LFKIF
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFKIF 766

BLAST of CSPI01G16460 vs. ExPASy Swiss-Prot
Match: P31414 (Pyrophosphate-energized vacuolar membrane proton pump 1 OS=Arabidopsis thaliana OX=3702 GN=AVP1 PE=1 SV=1)

HSP 1 Score: 1291.6 bits (3341), Expect = 0.0e+00
Identity = 677/765 (88.50%), Postives = 723/765 (94.51%), Query Frame = 0

Query: 5   ILPDLGAQIFIPLCAIVGILFSLVQWYYVSQVKLSS-ARDSANNNSSSAKNGYSDYLIEE 64
           +LP+L  +I +P+CA++GI FSL QWY VS+VKL+S    S++  +++ KNGY DYLIEE
Sbjct: 6   LLPELWTEILVPICAVIGIAFSLFQWYVVSRVKLTSDLGASSSGGANNGKNGYGDYLIEE 65

Query: 65  EEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFSTKP 124
           EEGVND +VV KCAEIQ AISEGATSFLFTEYKYVG+FMI FAA+IFVFLGSVEGFST  
Sbjct: 66  EEGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVIFVFLGSVEGFSTDN 125

Query: 125 QPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGK 184
           +PC+YD T+TCKPALATA FSTI+F+LGAVTSV+SGFLGMKIATYANARTTLEARKGVGK
Sbjct: 126 KPCTYDTTRTCKPALATAAFSTIAFVLGAVTSVLSGFLGMKIATYANARTTLEARKGVGK 185

Query: 185 AFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGEDWGGLFESITGYGLGGSSMALFG 244
           AFI AFRSGAVMGFLLAA+GLLVL+I IN+FK+YYG+DW GLFE+ITGYGLGGSSMALFG
Sbjct: 186 AFIVAFRSGAVMGFLLAASGLLVLYITINVFKIYYGDDWEGLFEAITGYGLGGSSMALFG 245

Query: 245 RVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 304
           RVGGGIYTKAADVGADLVGK+ERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE
Sbjct: 246 RVGGGIYTKAADVGADLVGKIERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 305

Query: 305 SSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIEPAL 364
           +SCAALVVASISSFG NH+FTAM YPL++SSMGILVCLITTLFATDFFEIK VKEIEPAL
Sbjct: 306 ASCAALVVASISSFGINHDFTAMCYPLLISSMGILVCLITTLFATDFFEIKLVKEIEPAL 365

Query: 365 KNQLIISTVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLIIGFV 424
           KNQLIISTVIMT GIAIV+WV +P  FTIFNFGTQKVV+NW+LFLCV VGLWAGLIIGFV
Sbjct: 366 KNQLIISTVIMTVGIAIVSWVGLPTSFTIFNFGTQKVVKNWQLFLCVCVGLWAGLIIGFV 425

Query: 425 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSFSFAAMYG 484
           TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA SIFVSFSFAAMYG
Sbjct: 426 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAISIFVSFSFAAMYG 485

Query: 485 IAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 544
           +AVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK
Sbjct: 486 VAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 545

Query: 545 GFAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAA 604
           GFAIGSAALVSLALFGAFVSRAG+  VD+LTPKV IGL+VGAMLPYWFSAMTMKSVGSAA
Sbjct: 546 GFAIGSAALVSLALFGAFVSRAGIHTVDVLTPKVIIGLLVGAMLPYWFSAMTMKSVGSAA 605

Query: 605 LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFG 664
           LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPG LVMLTPLIVG  FG
Sbjct: 606 LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGCLVMLTPLIVGFFFG 665

Query: 665 VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKAAVI 724
           VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAG SEHA++LGPKGS+PHKAAVI
Sbjct: 666 VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGVSEHAKSLGPKGSEPHKAAVI 725

Query: 725 GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF 769
           GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA+HGGILFK F
Sbjct: 726 GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGILFKYF 770

BLAST of CSPI01G16460 vs. ExPASy Swiss-Prot
Match: Q06572 (Pyrophosphate-energized vacuolar membrane proton pump OS=Hordeum vulgare OX=4513 PE=2 SV=2)

HSP 1 Score: 1262.3 bits (3265), Expect = 0.0e+00
Identity = 661/762 (86.75%), Postives = 718/762 (94.23%), Query Frame = 0

Query: 5   ILPDLGAQIFIPLCAIVGILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYLIEEE 64
           IL +LG +I IP+C ++GI+F++ QW+ VS+VK++    SA   ++ AKNGY DYLIEEE
Sbjct: 3   ILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPGAASA---AAGAKNGYGDYLIEEE 62

Query: 65  EGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFSTKPQ 124
           EG+NDHNVV+KCAEIQ AISEGATSFLFT Y+YVG+FM++FAA+IF+FLGS+EGFSTK Q
Sbjct: 63  EGLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGFSTKGQ 122

Query: 125 PCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGKA 184
           PC+Y K  TCKPAL TA FST SFLLGA+TS+VSGFLGMKIATYANARTTLEARKGVGKA
Sbjct: 123 PCTYSK-GTCKPALYTALFSTASFLLGAITSLVSGFLGMKIATYANARTTLEARKGVGKA 182

Query: 185 FITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGEDWGGLFESITGYGLGGSSMALFGR 244
           FITAFRSGAVMGFLL+++GL+VL+I IN+FK+YYG+DW GLFESITGYGLGGSSMALFGR
Sbjct: 183 FITAFRSGAVMGFLLSSSGLVVLYITINVFKMYYGDDWEGLFESITGYGLGGSSMALFGR 242

Query: 245 VGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAES 304
           VGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAES
Sbjct: 243 VGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAES 302

Query: 305 SCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIEPALK 364
           SCAALVVASISSFG NH+FTAM YPL+VSS+GI+VCL+TTLFATDFFEIKA  EIEPALK
Sbjct: 303 SCAALVVASISSFGINHDFTAMCYPLLVSSVGIIVCLLTTLFATDFFEIKAANEIEPALK 362

Query: 365 NQLIISTVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLIIGFVT 424
            QLIIST +MT G+A+++W+++PAKFTIFNFG QK V NW LF CVAVGLWAGLIIGFVT
Sbjct: 363 KQLIISTALMTVGVAVISWLALPAKFTIFNFGAQKEVSNWGLFFCVAVGLWAGLIIGFVT 422

Query: 425 EYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSFSFAAMYGI 484
           EYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA SI+VSFS AAMYGI
Sbjct: 423 EYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIYVSFSIAAMYGI 482

Query: 485 AVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKG 544
           A+AALGMLST+ATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKG
Sbjct: 483 AMAALGMLSTMATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKG 542

Query: 545 FAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAAL 604
           FAIGSAALVSLALFGAFVSRAGV VVD+L+PKVFIGLIVGAMLPYWFSAMTMKSVGSAAL
Sbjct: 543 FAIGSAALVSLALFGAFVSRAGVKVVDVLSPKVFIGLIVGAMLPYWFSAMTMKSVGSAAL 602

Query: 605 KMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFGV 664
           KMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVG LFGV
Sbjct: 603 KMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGTLFGV 662

Query: 665 ETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKAAVIG 724
           ETLSGVLAG+LVSGVQIAISASNTGGAWDNAKKYIEAG SEHAR+LGPKGSD HKAAVIG
Sbjct: 663 ETLSGVLAGALVSGVQIAISASNTGGAWDNAKKYIEAGNSEHARSLGPKGSDCHKAAVIG 722

Query: 725 DTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFK 767
           DTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA++GG+LFK
Sbjct: 723 DTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATYGGLLFK 760

BLAST of CSPI01G16460 vs. ExPASy Swiss-Prot
Match: Q72Q29 (Putative K(+)-stimulated pyrophosphate-energized sodium pump OS=Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni (strain Fiocruz L1-130) OX=267671 GN=hppA PE=3 SV=1)

HSP 1 Score: 691.0 bits (1782), Expect = 1.5e-197
Identity = 392/699 (56.08%), Postives = 503/699 (71.96%), Query Frame = 0

Query: 75  KCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGS--VEGFSTKPQPCSYDKTK 134
           K  EI +AISEGA +FL  EYK + +F+   A LI + L +   EGF+            
Sbjct: 42  KLLEISSAISEGAMAFLVREYKVISLFIAFMAVLIVLLLDNPGSEGFN------------ 101

Query: 135 TCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSG 194
                     ++ I+F+ GA+ S +SGF+GMKIAT  N RT   A+  + KAF  AF SG
Sbjct: 102 -------DGIYTAIAFVSGALISCISGFIGMKIATAGNVRTAEAAKSSMAKAFRVAFDSG 161

Query: 195 AVMGF---LLAANGLLVLFIAINLFKLYYGEDWGGLFESITGYGLGGSSMALFGRVGGGI 254
           AVMGF    LA  G++VLF+      +Y G +   L ES+ G+GLGGS++ALFGRVGGGI
Sbjct: 162 AVMGFGLVGLAILGMIVLFLVFT--GMYPGVEKHFLMESLAGFGLGGSAVALFGRVGGGI 221

Query: 255 YTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAAL 314
           YTKAADVGADLVGKVE+ IPEDDPRNPA IADNVGDNVGD+AGMG+DLFGS AE++CAAL
Sbjct: 222 YTKAADVGADLVGKVEKGIPEDDPRNPATIADNVGDNVGDVAGMGADLFGSCAEATCAAL 281

Query: 315 VVASISSFGNNHEFTAMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIEPALKNQLII 374
           V+ + +S   +    A+LYPL++S+ GI   ++T+  A     +K    +E ALK QL +
Sbjct: 282 VIGATAS-ALSGSVDALLYPLLISAFGIPASILTSFLA----RVKEDGNVESALKVQLWV 341

Query: 375 STVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLIIGFVTEYYTS 434
           ST+++   +  VT   +   F I      K +  W++++ + VGL++G+ IG VTEYYTS
Sbjct: 342 STLLVAGIMYFVTKTFMVDSFEI----AGKTITKWDVYISMVVGLFSGMFIGIVTEYYTS 401

Query: 435 NAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSFSFAAMYGIAVAAL 494
           ++Y PV++VA++  TGAATN+I+GL+LGY S +IP+  +  +I  +   A MYGIA+AAL
Sbjct: 402 HSYKPVREVAEASNTGAATNIIYGLSLGYHSSVIPVILLVITIVTANLLAGMYGIAIAAL 461

Query: 495 GMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKGFAIGS 554
           GM+STIA GL IDAYGP+SDNAGGIAEMA +   +R+RTD LDAAGNTTAAIGKGFAIGS
Sbjct: 462 GMISTIAIGLTIDAYGPVSDNAGGIAEMAELGKEVRDRTDTLDAAGNTTAAIGKGFAIGS 521

Query: 555 AALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVEE 614
           AAL SLALF AF++R     +++L  +VF GL+ GAMLP+ F+AMTMKSVG AA+ MVEE
Sbjct: 522 AALTSLALFAAFITRTHTTSLEVLNAEVFGGLMFGAMLPFLFTAMTMKSVGKAAVDMVEE 581

Query: 615 VRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFGVETLSG 674
           VR+QF  IPG+MEG  KPDY  CV IST A+++EMI PG LV+LTP++VG LFGV+TL+G
Sbjct: 582 VRKQFKEIPGIMEGKNKPDYKRCVDISTSAALREMILPGLLVLLTPILVGYLFGVKTLAG 641

Query: 675 VLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKAAVIGDTIGD 734
           VLAG+LV+GV +AISA+N+GG WDNAKKYIE       +  G KGSD HKAAV+GDT+GD
Sbjct: 642 VLAGALVAGVVLAISAANSGGGWDNAKKYIE-------KKAGGKGSDQHKAAVVGDTVGD 701

Query: 735 PLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF 769
           P KDTSGPS+NILIKLMA+ SLVFA FF   GG++FKIF
Sbjct: 702 PFKDTSGPSINILIKLMAITSLVFAEFFVQQGGLIFKIF 703

BLAST of CSPI01G16460 vs. ExPASy Swiss-Prot
Match: Q8F641 (Putative K(+)-stimulated pyrophosphate-energized sodium pump OS=Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) OX=189518 GN=hppA PE=3 SV=1)

HSP 1 Score: 691.0 bits (1782), Expect = 1.5e-197
Identity = 392/699 (56.08%), Postives = 503/699 (71.96%), Query Frame = 0

Query: 75  KCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGS--VEGFSTKPQPCSYDKTK 134
           K  EI +AISEGA +FL  EYK + +F+   A LI + L +   EGF+            
Sbjct: 42  KLLEISSAISEGAMAFLVREYKVISLFIAFMAVLIVLLLDNPGSEGFN------------ 101

Query: 135 TCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSG 194
                     ++ I+F+ GA+ S +SGF+GMKIAT  N RT   A+  + KAF  AF SG
Sbjct: 102 -------DGIYTAIAFVSGALISCISGFIGMKIATAGNVRTAEAAKSSMAKAFRVAFDSG 161

Query: 195 AVMGF---LLAANGLLVLFIAINLFKLYYGEDWGGLFESITGYGLGGSSMALFGRVGGGI 254
           AVMGF    LA  G++VLF+      +Y G +   L ES+ G+GLGGS++ALFGRVGGGI
Sbjct: 162 AVMGFGLVGLAILGMIVLFLVFT--GMYPGVEKHFLMESLAGFGLGGSAVALFGRVGGGI 221

Query: 255 YTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAAL 314
           YTKAADVGADLVGKVE+ IPEDDPRNPA IADNVGDNVGD+AGMG+DLFGS AE++CAAL
Sbjct: 222 YTKAADVGADLVGKVEKGIPEDDPRNPATIADNVGDNVGDVAGMGADLFGSCAEATCAAL 281

Query: 315 VVASISSFGNNHEFTAMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIEPALKNQLII 374
           V+ + +S   +    A+LYPL++S+ GI   ++T+  A     +K    +E ALK QL +
Sbjct: 282 VIGATAS-ALSGSVDALLYPLLISAFGIPASILTSFLA----RVKEDGNVESALKVQLWV 341

Query: 375 STVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLIIGFVTEYYTS 434
           ST+++   +  VT   +   F I      K +  W++++ + VGL++G+ IG VTEYYTS
Sbjct: 342 STLLVAGIMYFVTKTFMVDSFEI----AGKTITKWDVYISMVVGLFSGMFIGIVTEYYTS 401

Query: 435 NAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSFSFAAMYGIAVAAL 494
           ++Y PV++VA++  TGAATN+I+GL+LGY S +IP+  +  +I  +   A MYGIA+AAL
Sbjct: 402 HSYKPVREVAEASNTGAATNIIYGLSLGYHSSVIPVILLVITIVTANLLAGMYGIAIAAL 461

Query: 495 GMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKGFAIGS 554
           GM+STIA GL IDAYGP+SDNAGGIAEMA +   +R+RTD LDAAGNTTAAIGKGFAIGS
Sbjct: 462 GMISTIAIGLTIDAYGPVSDNAGGIAEMAELGKEVRDRTDTLDAAGNTTAAIGKGFAIGS 521

Query: 555 AALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVEE 614
           AAL SLALF AF++R     +++L  +VF GL+ GAMLP+ F+AMTMKSVG AA+ MVEE
Sbjct: 522 AALTSLALFAAFITRTHTTSLEVLNAEVFGGLMFGAMLPFLFTAMTMKSVGKAAVDMVEE 581

Query: 615 VRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFGVETLSG 674
           VR+QF  IPG+MEG  KPDY  CV IST A+++EMI PG LV+LTP++VG LFGV+TL+G
Sbjct: 582 VRKQFKEIPGIMEGKNKPDYKRCVDISTSAALREMILPGLLVLLTPILVGYLFGVKTLAG 641

Query: 675 VLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKAAVIGDTIGD 734
           VLAG+LV+GV +AISA+N+GG WDNAKKYIE       +  G KGSD HKAAV+GDT+GD
Sbjct: 642 VLAGALVAGVVLAISAANSGGGWDNAKKYIE-------KKAGGKGSDQHKAAVVGDTVGD 701

Query: 735 PLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF 769
           P KDTSGPS+NILIKLMA+ SLVFA FF   GG++FKIF
Sbjct: 702 PFKDTSGPSINILIKLMAITSLVFAEFFVQQGGLIFKIF 703

BLAST of CSPI01G16460 vs. ExPASy TrEMBL
Match: A0A0A0LTJ8 (H(+)-exporting diphosphatase OS=Cucumis sativus OX=3659 GN=Csa_1G212840 PE=3 SV=1)

HSP 1 Score: 1442.6 bits (3733), Expect = 0.0e+00
Identity = 768/768 (100.00%), Postives = 768/768 (100.00%), Query Frame = 0

Query: 1   MGATILPDLGAQIFIPLCAIVGILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL 60
           MGATILPDLGAQIFIPLCAIVGILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL
Sbjct: 1   MGATILPDLGAQIFIPLCAIVGILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL 60

Query: 61  IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS 120
           IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS 120

Query: 121 TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180
           TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGEDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGEDWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGEDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360

Query: 361 PALKNQLIISTVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLII 420
           PALKNQLIISTVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLII
Sbjct: 361 PALKNQLIISTVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSFSFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSFSFAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSFSFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF 769
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF 768

BLAST of CSPI01G16460 vs. ExPASy TrEMBL
Match: A0A5D3C3E3 (H(+)-exporting diphosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold163G00010 PE=3 SV=1)

HSP 1 Score: 1437.9 bits (3721), Expect = 0.0e+00
Identity = 763/768 (99.35%), Postives = 766/768 (99.74%), Query Frame = 0

Query: 1   MGATILPDLGAQIFIPLCAIVGILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL 60
           MGATILPDLGAQIFIPLCAI+GILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL
Sbjct: 1   MGATILPDLGAQIFIPLCAIIGILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL 60

Query: 61  IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS 120
           IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS 120

Query: 121 TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180
           TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGEDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAIN+FKLYYGEDWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINIFKLYYGEDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCL+TTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLVTTLFATDFFEIKAVKEIE 360

Query: 361 PALKNQLIISTVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLII 420
           PALKNQLIISTVIMTFGIAIVTWV VPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLII
Sbjct: 361 PALKNQLIISTVIMTFGIAIVTWVGVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSFSFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA SIFVSFSFAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFSFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF 769
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF 768

BLAST of CSPI01G16460 vs. ExPASy TrEMBL
Match: K9JA38 (H(+)-exporting diphosphatase OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 1437.9 bits (3721), Expect = 0.0e+00
Identity = 765/768 (99.61%), Postives = 766/768 (99.74%), Query Frame = 0

Query: 1   MGATILPDLGAQIFIPLCAIVGILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL 60
           MGA ILPDLGAQIFIPLCAIVGILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL
Sbjct: 1   MGAAILPDLGAQIFIPLCAIVGILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL 60

Query: 61  IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS 120
           IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS 120

Query: 121 TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180
           TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGEDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGEDWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGEDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360

Query: 361 PALKNQLIISTVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLII 420
           PALKNQLIISTVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLII
Sbjct: 361 PALKNQLIISTVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSFSFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSFSFAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSFSFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGVVV+DLLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVVVLDLLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHK 
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKT 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF 769
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF 768

BLAST of CSPI01G16460 vs. ExPASy TrEMBL
Match: A0A1S3B8Q5 (H(+)-exporting diphosphatase OS=Cucumis melo OX=3656 GN=LOC103487401 PE=3 SV=1)

HSP 1 Score: 1437.9 bits (3721), Expect = 0.0e+00
Identity = 763/768 (99.35%), Postives = 766/768 (99.74%), Query Frame = 0

Query: 1   MGATILPDLGAQIFIPLCAIVGILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL 60
           MGATILPDLGAQIFIPLCAI+GILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL
Sbjct: 1   MGATILPDLGAQIFIPLCAIIGILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL 60

Query: 61  IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS 120
           IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS 120

Query: 121 TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180
           TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGEDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAIN+FKLYYGEDWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINIFKLYYGEDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCL+TTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLVTTLFATDFFEIKAVKEIE 360

Query: 361 PALKNQLIISTVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLII 420
           PALKNQLIISTVIMTFGIAIVTWV VPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLII
Sbjct: 361 PALKNQLIISTVIMTFGIAIVTWVGVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSFSFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA SIFVSFSFAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFSFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF 769
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF 768

BLAST of CSPI01G16460 vs. ExPASy TrEMBL
Match: A0A5A7U4C7 (H(+)-exporting diphosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00680 PE=3 SV=1)

HSP 1 Score: 1434.9 bits (3713), Expect = 0.0e+00
Identity = 762/768 (99.22%), Postives = 765/768 (99.61%), Query Frame = 0

Query: 1   MGATILPDLGAQIFIPLCAIVGILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL 60
           MGATILPDLGAQIFIPLCAI+GILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL
Sbjct: 1   MGATILPDLGAQIFIPLCAIIGILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL 60

Query: 61  IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS 120
           IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS 120

Query: 121 TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180
           TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGEDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAIN+FKLYYGEDWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINIFKLYYGEDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCL+TTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLVTTLFATDFFEIKAVKEIE 360

Query: 361 PALKNQLIISTVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLII 420
           PALKNQLIISTVIMTFGIAIVTWV VPAKFTIFNFGTQKVVQNW LFLCVAVGLWAGLII
Sbjct: 361 PALKNQLIISTVIMTFGIAIVTWVGVPAKFTIFNFGTQKVVQNWLLFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSFSFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA SIFVSFSFAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFSFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF 769
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF 768

BLAST of CSPI01G16460 vs. NCBI nr
Match: KGN65098.1 (hypothetical protein Csa_004550 [Cucumis sativus])

HSP 1 Score: 1442.6 bits (3733), Expect = 0.0e+00
Identity = 768/768 (100.00%), Postives = 768/768 (100.00%), Query Frame = 0

Query: 1   MGATILPDLGAQIFIPLCAIVGILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL 60
           MGATILPDLGAQIFIPLCAIVGILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL
Sbjct: 1   MGATILPDLGAQIFIPLCAIVGILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL 60

Query: 61  IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS 120
           IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS 120

Query: 121 TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180
           TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGEDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGEDWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGEDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360

Query: 361 PALKNQLIISTVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLII 420
           PALKNQLIISTVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLII
Sbjct: 361 PALKNQLIISTVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSFSFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSFSFAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSFSFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF 769
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF 768

BLAST of CSPI01G16460 vs. NCBI nr
Match: NP_001295775.1 (pyrophosphate-energized vacuolar membrane proton pump [Cucumis sativus] >ADD51840.1 pyrophosphatase [Cucumis sativus])

HSP 1 Score: 1437.9 bits (3721), Expect = 0.0e+00
Identity = 765/768 (99.61%), Postives = 766/768 (99.74%), Query Frame = 0

Query: 1   MGATILPDLGAQIFIPLCAIVGILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL 60
           MGA ILPDLGAQIFIPLCAIVGILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL
Sbjct: 1   MGAAILPDLGAQIFIPLCAIVGILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL 60

Query: 61  IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS 120
           IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS 120

Query: 121 TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180
           TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGEDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGEDWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGEDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360

Query: 361 PALKNQLIISTVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLII 420
           PALKNQLIISTVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLII
Sbjct: 361 PALKNQLIISTVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSFSFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSFSFAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSFSFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGVVV+DLLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVVVLDLLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHK 
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKT 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF 769
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF 768

BLAST of CSPI01G16460 vs. NCBI nr
Match: XP_008443926.1 (PREDICTED: pyrophosphate-energized vacuolar membrane proton pump [Cucumis melo] >TYK06337.1 pyrophosphate-energized vacuolar membrane proton pump [Cucumis melo var. makuwa])

HSP 1 Score: 1437.9 bits (3721), Expect = 0.0e+00
Identity = 763/768 (99.35%), Postives = 766/768 (99.74%), Query Frame = 0

Query: 1   MGATILPDLGAQIFIPLCAIVGILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL 60
           MGATILPDLGAQIFIPLCAI+GILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL
Sbjct: 1   MGATILPDLGAQIFIPLCAIIGILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL 60

Query: 61  IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS 120
           IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS 120

Query: 121 TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180
           TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGEDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAIN+FKLYYGEDWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINIFKLYYGEDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCL+TTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLVTTLFATDFFEIKAVKEIE 360

Query: 361 PALKNQLIISTVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLII 420
           PALKNQLIISTVIMTFGIAIVTWV VPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLII
Sbjct: 361 PALKNQLIISTVIMTFGIAIVTWVGVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSFSFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA SIFVSFSFAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFSFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF 769
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF 768

BLAST of CSPI01G16460 vs. NCBI nr
Match: KAA0050084.1 (pyrophosphate-energized vacuolar membrane proton pump [Cucumis melo var. makuwa])

HSP 1 Score: 1434.9 bits (3713), Expect = 0.0e+00
Identity = 762/768 (99.22%), Postives = 765/768 (99.61%), Query Frame = 0

Query: 1   MGATILPDLGAQIFIPLCAIVGILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL 60
           MGATILPDLGAQIFIPLCAI+GILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL
Sbjct: 1   MGATILPDLGAQIFIPLCAIIGILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL 60

Query: 61  IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS 120
           IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS 120

Query: 121 TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180
           TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGEDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAIN+FKLYYGEDWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINIFKLYYGEDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCL+TTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLVTTLFATDFFEIKAVKEIE 360

Query: 361 PALKNQLIISTVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLII 420
           PALKNQLIISTVIMTFGIAIVTWV VPAKFTIFNFGTQKVVQNW LFLCVAVGLWAGLII
Sbjct: 361 PALKNQLIISTVIMTFGIAIVTWVGVPAKFTIFNFGTQKVVQNWLLFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSFSFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA SIFVSFSFAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFSFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF 769
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF 768

BLAST of CSPI01G16460 vs. NCBI nr
Match: XP_038878282.1 (pyrophosphate-energized vacuolar membrane proton pump [Benincasa hispida])

HSP 1 Score: 1403.7 bits (3632), Expect = 0.0e+00
Identity = 741/768 (96.48%), Postives = 757/768 (98.57%), Query Frame = 0

Query: 1   MGATILPDLGAQIFIPLCAIVGILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL 60
           MG TILPDLGA+IFIP+CAIVGILFSLVQWYYVSQVKLS ARDSANNNS++AKNGYSDYL
Sbjct: 1   MGVTILPDLGAEIFIPVCAIVGILFSLVQWYYVSQVKLSPARDSANNNSAAAKNGYSDYL 60

Query: 61  IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS 120
           IEEEEGVNDHNVV+KCAEIQ+AISEGATSFLFTEY YVGIFM+LFA LIFVFLGSVEGFS
Sbjct: 61  IEEEEGVNDHNVVVKCAEIQSAISEGATSFLFTEYYYVGIFMVLFAVLIFVFLGSVEGFS 120

Query: 121 TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180
           TKPQ CSYDKTKTCKPALATA FST+SF+LGA+TSVVSGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQVCSYDKTKTCKPALATAIFSTVSFILGAITSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGEDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYG+DWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFGNNHEFT MLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGNNHEFTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360

Query: 361 PALKNQLIISTVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLII 420
           PALK QLIISTV+MTFGIAIVTW++VP+KFTIFNFGTQKVVQNWELFLCVAVGLWAGLII
Sbjct: 361 PALKKQLIISTVLMTFGIAIVTWLAVPSKFTIFNFGTQKVVQNWELFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSFSFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA SIFVSFSFAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFSFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGV VVD+LTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVHVVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF 769
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFK F
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKFF 768

BLAST of CSPI01G16460 vs. TAIR 10
Match: AT1G15690.1 (Inorganic H pyrophosphatase family protein )

HSP 1 Score: 1291.6 bits (3341), Expect = 0.0e+00
Identity = 677/765 (88.50%), Postives = 723/765 (94.51%), Query Frame = 0

Query: 5   ILPDLGAQIFIPLCAIVGILFSLVQWYYVSQVKLSS-ARDSANNNSSSAKNGYSDYLIEE 64
           +LP+L  +I +P+CA++GI FSL QWY VS+VKL+S    S++  +++ KNGY DYLIEE
Sbjct: 6   LLPELWTEILVPICAVIGIAFSLFQWYVVSRVKLTSDLGASSSGGANNGKNGYGDYLIEE 65

Query: 65  EEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFSTKP 124
           EEGVND +VV KCAEIQ AISEGATSFLFTEYKYVG+FMI FAA+IFVFLGSVEGFST  
Sbjct: 66  EEGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVIFVFLGSVEGFSTDN 125

Query: 125 QPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGK 184
           +PC+YD T+TCKPALATA FSTI+F+LGAVTSV+SGFLGMKIATYANARTTLEARKGVGK
Sbjct: 126 KPCTYDTTRTCKPALATAAFSTIAFVLGAVTSVLSGFLGMKIATYANARTTLEARKGVGK 185

Query: 185 AFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGEDWGGLFESITGYGLGGSSMALFG 244
           AFI AFRSGAVMGFLLAA+GLLVL+I IN+FK+YYG+DW GLFE+ITGYGLGGSSMALFG
Sbjct: 186 AFIVAFRSGAVMGFLLAASGLLVLYITINVFKIYYGDDWEGLFEAITGYGLGGSSMALFG 245

Query: 245 RVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 304
           RVGGGIYTKAADVGADLVGK+ERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE
Sbjct: 246 RVGGGIYTKAADVGADLVGKIERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 305

Query: 305 SSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIEPAL 364
           +SCAALVVASISSFG NH+FTAM YPL++SSMGILVCLITTLFATDFFEIK VKEIEPAL
Sbjct: 306 ASCAALVVASISSFGINHDFTAMCYPLLISSMGILVCLITTLFATDFFEIKLVKEIEPAL 365

Query: 365 KNQLIISTVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLIIGFV 424
           KNQLIISTVIMT GIAIV+WV +P  FTIFNFGTQKVV+NW+LFLCV VGLWAGLIIGFV
Sbjct: 366 KNQLIISTVIMTVGIAIVSWVGLPTSFTIFNFGTQKVVKNWQLFLCVCVGLWAGLIIGFV 425

Query: 425 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSFSFAAMYG 484
           TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA SIFVSFSFAAMYG
Sbjct: 426 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAISIFVSFSFAAMYG 485

Query: 485 IAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 544
           +AVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK
Sbjct: 486 VAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 545

Query: 545 GFAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAA 604
           GFAIGSAALVSLALFGAFVSRAG+  VD+LTPKV IGL+VGAMLPYWFSAMTMKSVGSAA
Sbjct: 546 GFAIGSAALVSLALFGAFVSRAGIHTVDVLTPKVIIGLLVGAMLPYWFSAMTMKSVGSAA 605

Query: 605 LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFG 664
           LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPG LVMLTPLIVG  FG
Sbjct: 606 LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGCLVMLTPLIVGFFFG 665

Query: 665 VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKAAVI 724
           VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAG SEHA++LGPKGS+PHKAAVI
Sbjct: 666 VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGVSEHAKSLGPKGSEPHKAAVI 725

Query: 725 GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF 769
           GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA+HGGILFK F
Sbjct: 726 GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGILFKYF 770

BLAST of CSPI01G16460 vs. TAIR 10
Match: AT1G15690.2 (Inorganic H pyrophosphatase family protein )

HSP 1 Score: 1047.0 bits (2706), Expect = 7.6e-306
Identity = 549/628 (87.42%), Postives = 591/628 (94.11%), Query Frame = 0

Query: 5   ILPDLGAQIFIPLCAIVGILFSLVQWYYVSQVKLSS-ARDSANNNSSSAKNGYSDYLIEE 64
           +LP+L  +I +P+CA++GI FSL QWY VS+VKL+S    S++  +++ KNGY DYLIEE
Sbjct: 6   LLPELWTEILVPICAVIGIAFSLFQWYVVSRVKLTSDLGASSSGGANNGKNGYGDYLIEE 65

Query: 65  EEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFSTKP 124
           EEGVND +VV KCAEIQ AISEGATSFLFTEYKYVG+FMI FAA+IFVFLGSVEGFST  
Sbjct: 66  EEGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVIFVFLGSVEGFSTDN 125

Query: 125 QPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGK 184
           +PC+YD T+TCKPALATA FSTI+F+LGAVTSV+SGFLGMKIATYANARTTLEARKGVGK
Sbjct: 126 KPCTYDTTRTCKPALATAAFSTIAFVLGAVTSVLSGFLGMKIATYANARTTLEARKGVGK 185

Query: 185 AFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGEDWGGLFESITGYGLGGSSMALFG 244
           AFI AFRSGAVMGFLLAA+GLLVL+I IN+FK+YYG+DW GLFE+ITGYGLGGSSMALFG
Sbjct: 186 AFIVAFRSGAVMGFLLAASGLLVLYITINVFKIYYGDDWEGLFEAITGYGLGGSSMALFG 245

Query: 245 RVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 304
           RVGGGIYTKAADVGADLVGK+ERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE
Sbjct: 246 RVGGGIYTKAADVGADLVGKIERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 305

Query: 305 SSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIEPAL 364
           +SCAALVVASISSFG NH+FTAM YPL++SSMGILVCLITTLFATDFFEIK VKEIEPAL
Sbjct: 306 ASCAALVVASISSFGINHDFTAMCYPLLISSMGILVCLITTLFATDFFEIKLVKEIEPAL 365

Query: 365 KNQLIISTVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLIIGFV 424
           KNQLIISTVIMT GIAIV+WV +P  FTIFNFGTQKVV+NW+LFLCV VGLWAGLIIGFV
Sbjct: 366 KNQLIISTVIMTVGIAIVSWVGLPTSFTIFNFGTQKVVKNWQLFLCVCVGLWAGLIIGFV 425

Query: 425 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSFSFAAMYG 484
           TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA SIFVSFSFAAMYG
Sbjct: 426 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAISIFVSFSFAAMYG 485

Query: 485 IAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 544
           +AVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK
Sbjct: 486 VAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 545

Query: 545 GFAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAA 604
           GFAIGSAALVSLALFGAFVSRAG+  VD+LTPKV IGL+VGAMLPYWFSAMTMKSVGSAA
Sbjct: 546 GFAIGSAALVSLALFGAFVSRAGIHTVDVLTPKVIIGLLVGAMLPYWFSAMTMKSVGSAA 605

Query: 605 LKMVEEVRRQFNTIPGLMEGTAKPDYAT 632
           LKMVEEVRRQFNTIPGLMEGTAKPDYAT
Sbjct: 606 LKMVEEVRRQFNTIPGLMEGTAKPDYAT 633

BLAST of CSPI01G16460 vs. TAIR 10
Match: AT1G16780.1 (Inorganic H pyrophosphatase family protein )

HSP 1 Score: 416.8 bits (1070), Expect = 3.9e-116
Identity = 285/727 (39.20%), Postives = 408/727 (56.12%), Query Frame = 0

Query: 78  EIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFSTKPQPCSYDKTKTCKPA 137
           +I +AI +GA  FL T+Y  +     L A   FV L      +  PQ  +    +T    
Sbjct: 150 QISDAIRDGAEGFLRTQYGTISKMAFLLA---FVILCIYLFRNLTPQQEASGLGRT---- 209

Query: 138 LATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAVMGF 197
             +A  +  +FLLGA+ S ++G++GM ++  AN R +  AR+   +A   A R+G     
Sbjct: 210 -MSAYITVAAFLLGALCSGIAGYVGMWVSVRANVRVSSAARRSAREALQIAVRAGGFSAL 269

Query: 198 LLAANGLLVLFIAINLFKLYYGEDWGGLFE------SITGYGLGGSSMALFGRVGGGIYT 257
           ++    ++ + I  + F ++   D  G  +       + GYG G S +ALF ++GGGIYT
Sbjct: 270 VVVGMAVIGIAILYSTFYVWLDVDSPGSMKVTDLPLLLVGYGFGASFVALFAQLGGGIYT 329

Query: 258 KAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAALVV 317
           K ADVGADLVGKVE  IPEDDPRNPAVIAD VGDNVGD A  G+DLF S A    +A+++
Sbjct: 330 KGADVGADLVGKVEHGIPEDDPRNPAVIADLVGDNVGDCAARGADLFESIAAEIISAMIL 389

Query: 318 ASISSFGNNHEFTA--MLYPLIVSSMGILVCL--ITTLFATDFFEIKAVKEIEPALKNQ- 377
               +     E  +  +L+PL+V S  +++    I ++  T    +K+  E +P +  Q 
Sbjct: 390 GGTMAQKCKIEDPSGFILFPLVVHSFDLVISSIGILSIKGTRNASVKSPVE-DPMVVLQK 449

Query: 378 ---LIISTVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNW-ELFLCVAVGLWAGLIIGF 437
              L I   ++TFG A   W+            T++    W   F+C  VG+    +  +
Sbjct: 450 GYSLTIILAVLTFG-ASTRWL----------LYTEQAPSAWLNFFMCGLVGIITAYVFVW 509

Query: 438 VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSF------ 497
           ++ YYT   Y PV+ +A +  TG  TN+I G++LG +S  +P+  I+ +I  +F      
Sbjct: 510 ISRYYTDYKYEPVRTLALASSTGHGTNIIAGVSLGLESTALPVLVISVAIISAFWLGNTS 569

Query: 498 --------SFAAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERT 557
                       ++G AVA +GMLST A  L +D +GPI+DNAGGI EM+     +RE T
Sbjct: 570 GLIDEKGNPTGGLFGTAVATMGMLSTAAYVLTMDMFGPIADNAGGIVEMSQQPESVREIT 629

Query: 558 DALDAAGNTTAAIGKGFAIGSAALVSLALFGAFVSRAGVVV------VDLLTPKVFIGLI 617
           D LDA GNTT A  KGFAIGSAAL S  LF A++             VD+  P+VFIG +
Sbjct: 630 DVLDAVGNTTKATTKGFAIGSAALASFLLFSAYMDEVSAFANVSFKEVDIAIPEVFIGGL 689

Query: 618 VGAMLPYWFSAMTMKSVGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIK 677
           +GAML + FSA    +VG  A ++V EVRRQF   PG+M+   KPDY  CV I   ++++
Sbjct: 690 LGAMLIFLFSAWACAAVGRTAQEVVNEVRRQFIERPGIMDYKEKPDYGRCVAIVASSALR 749

Query: 678 EMIPPGALVMLTPLIVGILF------------GVETLSGVLAGSLVSGVQIAISASNTGG 737
           EMI PGAL +++P+ VG +F            G + ++ +L  + V G+ +A+  +  GG
Sbjct: 750 EMIKPGALAIISPIAVGFVFRILGYYTGQPLLGAKVVAAMLMFATVCGILMALFLNTAGG 809

Query: 738 AWDNAKKYIEAGTSEHARTLGPKGSDPHKAAVIGDTIGDPLKDTSGPSLNILIKLMAVES 758
           AWDNAKKYIE G       LG KGSD HKAAV GDT+GDP KDT+GPS+++LIK++A  +
Sbjct: 810 AWDNAKKYIETG------ALGGKGSDSHKAAVTGDTVGDPFKDTAGPSIHVLIKMLATIT 850

BLAST of CSPI01G16460 vs. TAIR 10
Match: AT1G78920.1 (vacuolar H+-pyrophosphatase 2 )

HSP 1 Score: 415.6 bits (1067), Expect = 8.6e-116
Identity = 283/726 (38.98%), Postives = 404/726 (55.65%), Query Frame = 0

Query: 78  EIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFSTKPQPCSYDKTKTCKPA 137
           EI +AI +GA  F  T+Y  +    IL A   FV L      S  PQ  +    +     
Sbjct: 101 EISDAIRDGAEGFFRTQYSTISKMAILLA---FVILCIYLFRSLTPQQEAAGLGRA---- 160

Query: 138 LATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAVMGF 197
             +A  +  +FLLGA+ S ++G++GM ++  AN R +  AR+   +A   A R+G     
Sbjct: 161 -MSAYITVAAFLLGALCSGIAGYVGMWVSVRANVRVSSAARRSAREALQIAVRAGGFSAL 220

Query: 198 LLAANGLLVLFIAINLFKLYYGEDWGGLFE------SITGYGLGGSSMALFGRVGGGIYT 257
           ++    ++ + I  + F ++ G    G          + GYG G S +ALF ++GGGIYT
Sbjct: 221 VVVGMAVIGIAILYSTFYVWLGVGSPGSMNVTDLPLLLVGYGFGASFVALFAQLGGGIYT 280

Query: 258 KAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAALVV 317
           K ADVGADLVGKVE+ IPEDDPRNPAVIAD VGDNVGD A  G+DLF S A    +A+++
Sbjct: 281 KGADVGADLVGKVEQGIPEDDPRNPAVIADLVGDNVGDCAARGADLFESIAAEIISAMIL 340

Query: 318 ASISSFGNNHEFTA--MLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIEPALK----- 377
               +     E  +  +L+PL+V S  +++  I  L      +      +E  +      
Sbjct: 341 GGTMAKKCKIEDPSGFILFPLVVHSFDLIISSIGILSIKGTRDASVKSPVEDPMAVLQKG 400

Query: 378 NQLIISTVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNWELF-LCVAVGLWAGLIIGFV 437
             L I   ++TFG A   W+            T++    W  F LC  VG+    I  ++
Sbjct: 401 YSLTIILAVITFG-ASTRWL----------LYTEQAPSAWFNFALCGLVGIITAYIFVWI 460

Query: 438 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSF------- 497
           ++YYT   + PV+ +A +  TG  TN+I G++LG +S  +P+  I+ +I  ++       
Sbjct: 461 SKYYTDYKHEPVRTLALASSTGHGTNIIAGVSLGLESTALPVLTISVAIISAYWLGNTSG 520

Query: 498 -------SFAAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTD 557
                      ++G AVA +GMLST A  L +D +GPI+DNAGGI EM+     +RE TD
Sbjct: 521 LVDENGIPTGGLFGTAVATMGMLSTAAYVLTMDMFGPIADNAGGIVEMSQQPESVREITD 580

Query: 558 ALDAAGNTTAAIGKGFAIGSAALVSLALFGAFVSRAGVVV------VDLLTPKVFIGLIV 617
            LDA GNTT A  KGFAIGSAAL S  LF A++             VD+  P+VF+G ++
Sbjct: 581 LLDAVGNTTKATTKGFAIGSAALASFLLFSAYMDEVSAFANVSFKEVDIAIPEVFVGGLL 640

Query: 618 GAMLPYWFSAMTMKSVGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKE 677
           GAML + FSA    +VG  A ++V EVRRQF   PG+ME   KPDY+ CV I   A+++E
Sbjct: 641 GAMLIFLFSAWACAAVGRTAQEVVNEVRRQFIERPGIMEYKEKPDYSRCVAIVASAALRE 700

Query: 678 MIPPGALVMLTPLIVGILF------------GVETLSGVLAGSLVSGVQIAISASNTGGA 737
           MI PGAL + +P++VG++F            G + ++ +L  + V G+ +A+  +  GGA
Sbjct: 701 MIKPGALAIASPIVVGLVFRILGYYTGQPLLGAKVVASMLMFATVCGILMALFLNTAGGA 760

Query: 738 WDNAKKYIEAGTSEHARTLGPKGSDPHKAAVIGDTIGDPLKDTSGPSLNILIKLMAVESL 758
           WDNAKKYIE G       LG KGS+ HKAAV GDT+GDP KDT+GPS+++LIK++A  +L
Sbjct: 761 WDNAKKYIETG------ALGGKGSEAHKAAVTGDTVGDPFKDTAGPSIHVLIKMLATITL 801

BLAST of CSPI01G16460 vs. TAIR 10
Match: AT1G78920.2 (vacuolar H+-pyrophosphatase 2 )

HSP 1 Score: 415.6 bits (1067), Expect = 8.6e-116
Identity = 283/726 (38.98%), Postives = 404/726 (55.65%), Query Frame = 0

Query: 78  EIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFSTKPQPCSYDKTKTCKPA 137
           EI +AI +GA  F  T+Y  +    IL A   FV L      S  PQ  +    +     
Sbjct: 101 EISDAIRDGAEGFFRTQYSTISKMAILLA---FVILCIYLFRSLTPQQEAAGLGRA---- 160

Query: 138 LATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAVMGF 197
             +A  +  +FLLGA+ S ++G++GM ++  AN R +  AR+   +A   A R+G     
Sbjct: 161 -MSAYITVAAFLLGALCSGIAGYVGMWVSVRANVRVSSAARRSAREALQIAVRAGGFSAL 220

Query: 198 LLAANGLLVLFIAINLFKLYYGEDWGGLFE------SITGYGLGGSSMALFGRVGGGIYT 257
           ++    ++ + I  + F ++ G    G          + GYG G S +ALF ++GGGIYT
Sbjct: 221 VVVGMAVIGIAILYSTFYVWLGVGSPGSMNVTDLPLLLVGYGFGASFVALFAQLGGGIYT 280

Query: 258 KAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAALVV 317
           K ADVGADLVGKVE+ IPEDDPRNPAVIAD VGDNVGD A  G+DLF S A    +A+++
Sbjct: 281 KGADVGADLVGKVEQGIPEDDPRNPAVIADLVGDNVGDCAARGADLFESIAAEIISAMIL 340

Query: 318 ASISSFGNNHEFTA--MLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIEPALK----- 377
               +     E  +  +L+PL+V S  +++  I  L      +      +E  +      
Sbjct: 341 GGTMAKKCKIEDPSGFILFPLVVHSFDLIISSIGILSIKGTRDASVKSPVEDPMAVLQKG 400

Query: 378 NQLIISTVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNWELF-LCVAVGLWAGLIIGFV 437
             L I   ++TFG A   W+            T++    W  F LC  VG+    I  ++
Sbjct: 401 YSLTIILAVITFG-ASTRWL----------LYTEQAPSAWFNFALCGLVGIITAYIFVWI 460

Query: 438 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSF------- 497
           ++YYT   + PV+ +A +  TG  TN+I G++LG +S  +P+  I+ +I  ++       
Sbjct: 461 SKYYTDYKHEPVRTLALASSTGHGTNIIAGVSLGLESTALPVLTISVAIISAYWLGNTSG 520

Query: 498 -------SFAAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTD 557
                      ++G AVA +GMLST A  L +D +GPI+DNAGGI EM+     +RE TD
Sbjct: 521 LVDENGIPTGGLFGTAVATMGMLSTAAYVLTMDMFGPIADNAGGIVEMSQQPESVREITD 580

Query: 558 ALDAAGNTTAAIGKGFAIGSAALVSLALFGAFVSRAGVVV------VDLLTPKVFIGLIV 617
            LDA GNTT A  KGFAIGSAAL S  LF A++             VD+  P+VF+G ++
Sbjct: 581 LLDAVGNTTKATTKGFAIGSAALASFLLFSAYMDEVSAFANVSFKEVDIAIPEVFVGGLL 640

Query: 618 GAMLPYWFSAMTMKSVGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKE 677
           GAML + FSA    +VG  A ++V EVRRQF   PG+ME   KPDY+ CV I   A+++E
Sbjct: 641 GAMLIFLFSAWACAAVGRTAQEVVNEVRRQFIERPGIMEYKEKPDYSRCVAIVASAALRE 700

Query: 678 MIPPGALVMLTPLIVGILF------------GVETLSGVLAGSLVSGVQIAISASNTGGA 737
           MI PGAL + +P++VG++F            G + ++ +L  + V G+ +A+  +  GGA
Sbjct: 701 MIKPGALAIASPIVVGLVFRILGYYTGQPLLGAKVVASMLMFATVCGILMALFLNTAGGA 760

Query: 738 WDNAKKYIEAGTSEHARTLGPKGSDPHKAAVIGDTIGDPLKDTSGPSLNILIKLMAVESL 758
           WDNAKKYIE G       LG KGS+ HKAAV GDT+GDP KDT+GPS+++LIK++A  +L
Sbjct: 761 WDNAKKYIETG------ALGGKGSEAHKAAVTGDTVGDPFKDTAGPSIHVLIKMLATITL 801