Tan0020528.1 (mRNA) Snake gourd v1

Overview
NameTan0020528.1
TypemRNA
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionProteinase inhibitor 1
LocationLG07: 6793894 .. 6794453 (+)
Sequence length454
RNA-Seq ExpressionTan0020528.1
SyntenyTan0020528.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAGAGAAGAAAAAAAAATTATTTTATTTATTCTCTAAACTTGAAAGAGCATGTGACCAAGTTAGCTATAAATAAAGCTACTTAACGAAGAATAAAGCATCGATCTCTCATTCCAGTTTTAACGAATAGATAGCAATACCAAGATGAATAGTAAGTAAAAAAAATCAGATTATATTTTTGTTGAGTTTTTTTTTTCTTAATTTTTGGTTGAACATGTATATATTCTATATTATATTATTTTTTTGCATGATTTCAGGAAAATCATGGCCGGAACTGGAATTCGTGGATGCTGTGACGGTAATCAATTACATAAAGAAAACAGATCCTGAGTTGAATATAGTTATACTGTTAAATGGAAGTCCAGTGACAAAAGACTTGAGGTTGGATCGAGTTCGACTTTTTGTTAACATCAATAATATCGTGATGAATGTTCCTACCACTGGTTGAAATATCAAAAATATATGTATGTACTGATCTTACCAGAGGTTAAAATGTCTTAAATGCATATGTTTAGATATAATATTTAAATAAAGTAGAATCTATTTCTACTACTTTAC

mRNA sequence

AAAAAAGAGAAGAAAAAAAAATTATTTTATTTATTCTCTAAACTTGAAAGAGCATGTGACCAAGTTAGCTATAAATAAAGCTACTTAACGAAGAATAAAGCATCGATCTCTCATTCCAGTTTTAACGAATAGATAGCAATACCAAGATGAATAGAAAATCATGGCCGGAACTGGAATTCGTGGATGCTGTGACGGTAATCAATTACATAAAGAAAACAGATCCTGAGTTGAATATAGTTATACTGTTAAATGGAAGTCCAGTGACAAAAGACTTGAGGTTGGATCGAGTTCGACTTTTTGTTAACATCAATAATATCGTGATGAATGTTCCTACCACTGGTTGAAATATCAAAAATATATGTATGTACTGATCTTACCAGAGGTTAAAATGTCTTAAATGCATATGTTTAGATATAATATTTAAATAAAGTAGAATCTATTTCTACTACTTTAC

Coding sequence (CDS)

ATGAATAGAAAATCATGGCCGGAACTGGAATTCGTGGATGCTGTGACGGTAATCAATTACATAAAGAAAACAGATCCTGAGTTGAATATAGTTATACTGTTAAATGGAAGTCCAGTGACAAAAGACTTGAGGTTGGATCGAGTTCGACTTTTTGTTAACATCAATAATATCGTGATGAATGTTCCTACCACTGGTTGA

Protein sequence

MNRKSWPELEFVDAVTVINYIKKTDPELNIVILLNGSPVTKDLRLDRVRLFVNINNIVMNVPTTG
Homology
BLAST of Tan0020528.1 vs. ExPASy Swiss-Prot
Match: P16231 (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 2.9e-09
Identity = 34/61 (55.74%), Postives = 42/61 (68.85%), Query Frame = 0

Query: 3   RKSWPELEFVDAVTVINYIKKTDPEL-NIVILLNGSPVTKDLRLDRVRLFVNINNIVMNV 62
           ++ WPEL  V A+     I+K +P + NI ILLNGSPVTKD R DRVRLFVNI   V+ +
Sbjct: 48  KQFWPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGDVVQI 107

BLAST of Tan0020528.1 vs. ExPASy Swiss-Prot
Match: Q03199 (Proteinase inhibitor I-B OS=Nicotiana tabacum OX=4097 GN=TIMPA PE=2 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 3.2e-08
Identity = 29/66 (43.94%), Postives = 44/66 (66.67%), Query Frame = 0

Query: 1   MNRKSWPELEFVDAVTVINYIKKTDPEL-NIVILLNGSPVTKDLRLDRVRLFVNINNIVM 60
           + ++ WPEL    A   +  I+K +P+L N+  +LNG+PVT+DLR +RVRLFVN+ + V+
Sbjct: 42  VTKERWPELLGTPAKFAMQIIQKENPKLTNVQTVLNGTPVTEDLRCNRVRLFVNVLDFVV 101

Query: 61  NVPTTG 66
             P  G
Sbjct: 102 QTPQVG 107

BLAST of Tan0020528.1 vs. ExPASy Swiss-Prot
Match: Q03198 (Proteinase inhibitor I-A OS=Nicotiana tabacum OX=4097 GN=TIMPB PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 5.4e-08
Identity = 28/66 (42.42%), Postives = 43/66 (65.15%), Query Frame = 0

Query: 1   MNRKSWPELEFVDAVTVINYIKKTDPEL-NIVILLNGSPVTKDLRLDRVRLFVNINNIVM 60
           + ++ WPEL    A   +  I+K +P+L N+  +LNG PVT+DLR +RVRLFVN+ + ++
Sbjct: 42  VTKERWPELLGTPAKFAMQIIQKENPKLTNVQTILNGGPVTEDLRCNRVRLFVNVLDFIV 101

Query: 61  NVPTTG 66
             P  G
Sbjct: 102 QTPQIG 107

BLAST of Tan0020528.1 vs. ExPASy Swiss-Prot
Match: Q02214 (Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 5.4e-08
Identity = 30/64 (46.88%), Postives = 43/64 (67.19%), Query Frame = 0

Query: 3  RKSWPELEFVDAVTVINYIKKTDPEL-NIVILLNGSPVTKDLRLDRVRLFVNINNIVMNV 62
          +++WPEL  V A      I+K + +L N+  +LNGSPVTKD R +RVRLFVN+ + V+ +
Sbjct: 31 KETWPELIGVPAKFAREIIQKENSKLTNVPSVLNGSPVTKDFRCERVRLFVNVLDFVVQI 90

Query: 63 PTTG 66
          P  G
Sbjct: 91 PRVG 94

BLAST of Tan0020528.1 vs. ExPASy Swiss-Prot
Match: P20076 (Ethylene-responsive proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 PE=3 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 7.1e-08
Identity = 31/66 (46.97%), Postives = 43/66 (65.15%), Query Frame = 0

Query: 1   MNRKSWPELEFVDAVTVINYIKKTDPEL-NIVILLNGSPVTKDLRLDRVRLFVNINNIVM 60
           + ++SWPEL    A      I+K +P+L N+  LLNGS  T+DLR +RVRLFVN+ +IV+
Sbjct: 54  VTKESWPELLGTPAKFAKQIIQKENPKLTNVETLLNGSAFTEDLRCNRVRLFVNLLDIVV 113

Query: 61  NVPTTG 66
             P  G
Sbjct: 114 QTPKVG 119

BLAST of Tan0020528.1 vs. NCBI nr
Match: KAG6571807.1 (hypothetical protein SDJN03_28535, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 75.5 bits (184), Expect = 1.9e-10
Identity = 39/62 (62.90%), Postives = 44/62 (70.97%), Query Frame = 0

Query: 4  KSWPELEFVDAVTVINYIKKTDPELNIVILLNGSPVTKDLRLDRVRLFVNINNIVMNVPT 63
          KSWPEL  + A TV N IK+  PE  IVILL GSPVTKDL+  RVRLFVNI  IV+ +P 
Sbjct: 6  KSWPELNLMQASTVENIIKRQHPEFQIVILLAGSPVTKDLKHGRVRLFVNILGIVVEIPR 65

Query: 64 TG 66
           G
Sbjct: 66 EG 67

BLAST of Tan0020528.1 vs. NCBI nr
Match: KGN60477.1 (hypothetical protein Csa_001998 [Cucumis sativus])

HSP 1 Score: 74.7 bits (182), Expect = 3.3e-10
Identity = 38/62 (61.29%), Postives = 44/62 (70.97%), Query Frame = 0

Query: 4  KSWPELEFVDAVTVINYIKKTDPELNIVILLNGSPVTKDLRLDRVRLFVNINNIVMNVPT 63
          KSWP L F+DA TV N IKK +P+  I ILL GSPVTKDL+  RVRLF N+  IV+ VP 
Sbjct: 10 KSWPALNFMDARTVANIIKKENPDFQIFILLAGSPVTKDLKHGRVRLFTNMEEIVVIVPQ 69

Query: 64 TG 66
           G
Sbjct: 70 EG 71

BLAST of Tan0020528.1 vs. NCBI nr
Match: XP_011654473.1 (inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetical protein Csa_018430 [Cucumis sativus])

HSP 1 Score: 70.1 bits (170), Expect = 8.0e-09
Identity = 32/63 (50.79%), Postives = 48/63 (76.19%), Query Frame = 0

Query: 3  RKSWPELEFVDAVTVINYIKKTDPELNIVILLNGSPVTKDLRLDRVRLFVNINNIVMNVP 62
          + SWPEL FV +   +++I++  P++  V+LL GSPVT+DLR +RVR+FVN+N+ V+ VP
Sbjct: 13 KSSWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVTEDLRPNRVRIFVNMNDRVVEVP 72

Query: 63 TTG 66
           TG
Sbjct: 73 RTG 75

BLAST of Tan0020528.1 vs. NCBI nr
Match: KGN60478.1 (hypothetical protein Csa_001396 [Cucumis sativus])

HSP 1 Score: 66.2 bits (160), Expect = 1.2e-07
Identity = 34/64 (53.12%), Postives = 42/64 (65.62%), Query Frame = 0

Query: 2  NRKSWPELEFVDAVTVINYIKKTDPELNIVILLNGSPVTKDLRLDRVRLFVNINNIVMNV 61
          N KSWP L+F+DA TV N IK  +P   IV +  G+PVTKD+   RVRL V+ N  V+NV
Sbjct: 6  NLKSWPALKFLDAQTVANAIKTENPNFEIVTVPEGTPVTKDIIPGRVRLVVDANGKVVNV 65

Query: 62 PTTG 66
          P  G
Sbjct: 66 PQEG 69

BLAST of Tan0020528.1 vs. NCBI nr
Match: CAF3779213.1 (unnamed protein product [Rotaria sordida])

HSP 1 Score: 65.5 bits (158), Expect = 2.0e-07
Identity = 32/65 (49.23%), Postives = 44/65 (67.69%), Query Frame = 0

Query: 1   MNRKSWPELEFVDAVTVINYIKKTDPELNIVILLNGSPVTKDLRLDRVRLFVNINNIVMN 60
           +N+  WPEL   +   V+N IKK     N+VI+  GSP+T D R++RVR+FVN N IV++
Sbjct: 482 LNKHRWPELIGKNGQEVVNIIKKETGFTNVVIVKEGSPITLDYRVNRVRVFVNDNGIVVH 541

Query: 61  VPTTG 66
           VPT G
Sbjct: 542 VPTIG 546

BLAST of Tan0020528.1 vs. ExPASy TrEMBL
Match: A0A0A0LIF6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914570 PE=3 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.6e-10
Identity = 38/62 (61.29%), Postives = 44/62 (70.97%), Query Frame = 0

Query: 4  KSWPELEFVDAVTVINYIKKTDPELNIVILLNGSPVTKDLRLDRVRLFVNINNIVMNVPT 63
          KSWP L F+DA TV N IKK +P+  I ILL GSPVTKDL+  RVRLF N+  IV+ VP 
Sbjct: 10 KSWPALNFMDARTVANIIKKENPDFQIFILLAGSPVTKDLKHGRVRLFTNMEEIVVIVPQ 69

Query: 64 TG 66
           G
Sbjct: 70 EG 71

BLAST of Tan0020528.1 vs. ExPASy TrEMBL
Match: A0A0A0KPI0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 3.9e-09
Identity = 32/63 (50.79%), Postives = 48/63 (76.19%), Query Frame = 0

Query: 3  RKSWPELEFVDAVTVINYIKKTDPELNIVILLNGSPVTKDLRLDRVRLFVNINNIVMNVP 62
          + SWPEL FV +   +++I++  P++  V+LL GSPVT+DLR +RVR+FVN+N+ V+ VP
Sbjct: 13 KSSWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVTEDLRPNRVRIFVNMNDRVVEVP 72

Query: 63 TTG 66
           TG
Sbjct: 73 RTG 75

BLAST of Tan0020528.1 vs. ExPASy TrEMBL
Match: A0A0A0LFD4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914580 PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 5.6e-08
Identity = 34/64 (53.12%), Postives = 42/64 (65.62%), Query Frame = 0

Query: 2  NRKSWPELEFVDAVTVINYIKKTDPELNIVILLNGSPVTKDLRLDRVRLFVNINNIVMNV 61
          N KSWP L+F+DA TV N IK  +P   IV +  G+PVTKD+   RVRL V+ N  V+NV
Sbjct: 6  NLKSWPALKFLDAQTVANAIKTENPNFEIVTVPEGTPVTKDIIPGRVRLVVDANGKVVNV 65

Query: 62 PTTG 66
          P  G
Sbjct: 66 PQEG 69

BLAST of Tan0020528.1 vs. ExPASy TrEMBL
Match: A0A1S3YJI6 (trypsin inhibitor 1-like OS=Nicotiana tabacum OX=4097 GN=LOC107776717 PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.6e-07
Identity = 34/66 (51.52%), Postives = 47/66 (71.21%), Query Frame = 0

Query: 1  MNRKSWPELEFVDAVTVINYIKKTDPEL-NIVILLNGSPVTKDLRLDRVRLFVNINNIVM 60
          + +++WPEL  V A      I+K +P+L N+  LLNGSPVTKDLR +RVRLFVN+ +IV+
Sbjct: 29 VKKETWPELIGVPAKFAREIIQKENPKLTNVPSLLNGSPVTKDLRCNRVRLFVNVLDIVV 88

Query: 61 NVPTTG 66
           +P  G
Sbjct: 89 QLPRVG 94

BLAST of Tan0020528.1 vs. ExPASy TrEMBL
Match: A0A6N2AU12 (Uncharacterized protein OS=Solanum chilense OX=4083 GN=EJD97_023458 PE=3 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 2.1e-07
Identity = 34/61 (55.74%), Postives = 43/61 (70.49%), Query Frame = 0

Query: 3   RKSWPELEFVDAVTVINYIKKTDPEL-NIVILLNGSPVTKDLRLDRVRLFVNINNIVMNV 62
           ++ WPEL  V A+     I+K +P + NI ILLNGSPVTKD R DRVRLFVNI  +V+ +
Sbjct: 41  KQFWPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGVVVQI 100

BLAST of Tan0020528.1 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 57.8 bits (138), Expect = 3.8e-09
Identity = 27/63 (42.86%), Postives = 40/63 (63.49%), Query Frame = 0

Query: 3  RKSWPELEFVDAVTVINYIKKTDPELNIVILLNGSPVTKDLRLDRVRLFVNINNIVMNVP 62
          + SWPEL   +       I++ +P +N  ++L+GSPVT D R DRVR+FV+ N IV+  P
Sbjct: 8  KNSWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGNRIVVKTP 67

Query: 63 TTG 66
           +G
Sbjct: 68 KSG 70

BLAST of Tan0020528.1 vs. TAIR 10
Match: AT2G38900.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 45.4 bits (106), Expect = 2.0e-05
Identity = 24/65 (36.92%), Postives = 39/65 (60.00%), Query Frame = 0

Query: 1  MNRKSWPELEFVDAVTVINYIKKTDPELNIVILLNGSPVTKDLRLDRVRLFVNINNIVMN 60
          + + SWPEL   +     + IK  +  LN+V++ +G+ VT+DL   RVR++V+   IV+ 
Sbjct: 9  IGKNSWPELLGTNGDYAASVIKGENSSLNVVVVSDGNYVTEDLSCYRVRVWVDEIRIVVR 68

Query: 61 VPTTG 66
           PT G
Sbjct: 69 NPTAG 73

BLAST of Tan0020528.1 vs. TAIR 10
Match: AT2G38900.2 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 45.4 bits (106), Expect = 2.0e-05
Identity = 24/65 (36.92%), Postives = 39/65 (60.00%), Query Frame = 0

Query: 1  MNRKSWPELEFVDAVTVINYIKKTDPELNIVILLNGSPVTKDLRLDRVRLFVNINNIVMN 60
          + + SWPEL   +     + IK  +  LN+V++ +G+ VT+DL   RVR++V+   IV+ 
Sbjct: 24 IGKNSWPELLGTNGDYAASVIKGENSSLNVVVVSDGNYVTEDLSCYRVRVWVDEIRIVVR 83

Query: 61 VPTTG 66
           PT G
Sbjct: 84 NPTAG 88

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P162312.9e-0955.74Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1[more]
Q031993.2e-0843.94Proteinase inhibitor I-B OS=Nicotiana tabacum OX=4097 GN=TIMPA PE=2 SV=1[more]
Q031985.4e-0842.42Proteinase inhibitor I-A OS=Nicotiana tabacum OX=4097 GN=TIMPB PE=2 SV=1[more]
Q022145.4e-0846.88Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1[more]
P200767.1e-0846.97Ethylene-responsive proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 PE=3 ... [more]
Match NameE-valueIdentityDescription
KAG6571807.11.9e-1062.90hypothetical protein SDJN03_28535, partial [Cucurbita argyrosperma subsp. sorori... [more]
KGN60477.13.3e-1061.29hypothetical protein Csa_001998 [Cucumis sativus][more]
XP_011654473.18.0e-0950.79inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetic... [more]
KGN60478.11.2e-0753.13hypothetical protein Csa_001396 [Cucumis sativus][more]
CAF3779213.12.0e-0749.23unnamed protein product [Rotaria sordida][more]
Match NameE-valueIdentityDescription
A0A0A0LIF61.6e-1061.29Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914570 PE=3 SV=1[more]
A0A0A0KPI03.9e-0950.79Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1[more]
A0A0A0LFD45.6e-0853.13Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914580 PE=3 SV=1[more]
A0A1S3YJI61.6e-0751.52trypsin inhibitor 1-like OS=Nicotiana tabacum OX=4097 GN=LOC107776717 PE=3 SV=1[more]
A0A6N2AU122.1e-0755.74Uncharacterized protein OS=Solanum chilense OX=4083 GN=EJD97_023458 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G38870.13.8e-0942.86Serine protease inhibitor, potato inhibitor I-type family protein [more]
AT2G38900.12.0e-0536.92Serine protease inhibitor, potato inhibitor I-type family protein [more]
AT2G38900.22.0e-0536.92Serine protease inhibitor, potato inhibitor I-type family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000864Proteinase inhibitor I13, potato inhibitor IPRINTSPR00292POTATOINHBTRcoord: 5..18
score: 35.4
coord: 30..41
score: 53.99
coord: 42..52
score: 63.24
IPR000864Proteinase inhibitor I13, potato inhibitor IPFAMPF00280potato_inhibitcoord: 4..65
e-value: 2.8E-16
score: 59.9
IPR000864Proteinase inhibitor I13, potato inhibitor IPANTHERPTHR33091PROTEIN, PUTATIVE, EXPRESSED-RELATEDcoord: 3..65
NoneNo IPR availableGENE3D3.30.10.10Trypsin Inhibitor V, subunit Acoord: 1..65
e-value: 6.2E-18
score: 66.8
NoneNo IPR availablePANTHERPTHR33091:SF83ETHYLENE-RESPONSIVE PROTEINASE INHIBITOR 1coord: 3..65
IPR036354Proteinase inhibitor I13, potato inhibitor I superfamilySUPERFAMILY54654CI-2 family of serine protease inhibitorscoord: 3..65

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Tan0020528Tan0020528gene


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Tan0020528.1-five_prime_utrTan0020528.1-five_prime_utr-LG07:6793894..6794039five_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Tan0020528.1-exonTan0020528.1-exon-LG07:6793894..6794046exon
Tan0020528.1-exonTan0020528.1-exon-LG07:6794153..6794453exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Tan0020528.1-cdsTan0020528.1-cds-LG07:6794040..6794046CDS
Tan0020528.1-cdsTan0020528.1-cds-LG07:6794153..6794343CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Tan0020528.1-three_prime_utrTan0020528.1-three_prime_utr-LG07:6794344..6794453three_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Tan0020528.1Tan0020528.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009611 response to wounding
molecular_function GO:0004867 serine-type endopeptidase inhibitor activity