Tan0000631.3 (mRNA) Snake gourd v1

Overview


Name	Tan0000631.3
Type	mRNA
Organism	Trichosanthes anguina (Snake gourd v1)
Description	DNA-directed RNA polymerases II and V subunit 8A-like
Location	LG01: 13183831 .. 13187375 (+)
Sequence length	1158
RNA-Seq Expression	Tan0000631.3
Synteny	Tan0000631.3

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAACCCTAGTCCCTCACCGTTAAAAAAAAAAAAACCCTATACCCCTCATCTTTCATTTTCTACCCCCTTCTTCCCGCCGGCTCTTCCTTTCTTTCAGCAGCTCACTGATTCCGCCGCCGTCGTTCTCCTGCATGTTCCTGCGCCTCCTCCGTCATAGCGGTTTGCACTGATTTTCTACTCTCTTGTTGTTATTGGTATTCTCCCAAATTCCATCCATGTCTTTCTTCTCACTTGAAAAACTTAAATTAGGACTTAGTCTAGTGAAAGAATAGAGTTGTTTTTTTTTTTTTGGTCTCGGCTGAAAATTTCATTGTTTCTAGTCTCCCACATCTTCCAAAGCATCCCCCACTGTGAATTTTTGAGCATCTCCCTCCTTTATTTGTCTCGAATCAGTCGAAGTAATCTATCACACACCAATTTTCCCTATTAAACAAAAATTCCCAGATATTAGAATTAAAGTTGTTATAACCAAATTTCTTTTCGAAACTTACACCGCATGGCAGGTAGTTTCGATTTTACTCTTTTTGTTTTTAGCGTCAACTTGTTCAATACAATCTGGTCCTGGACAGACTTGGAAGTTCTTGTATTGGTTGTTTTCTGGGGTTTGAACGTTGGAATTTGCTTTATCAATTTTCTACTGTAATTTTGCGTTTGCGCTGAGTGCTTTTTTTAATTCCCAATTCAAATACTTTCTCCAACCCCTCTACTATTTCATTCTGATTCTGCTCAAATTCTAGTTGGCGCTCTTTTTCCCTGCTTTCTATTTATTATTTGTTGCTTGCCAACCGACTCAGATTTGCATCTTGAGGAGTAAATTCTCCATAAACATGGTGGAGCTTCTATTTGATGATATCTTCAAGGTTAAGTGTGTCAACCCAGATGGTAAAAAGTATGATAAAGGTACTTGCCCTCAGTCTTGTTACTGCTCTCGCATTCTTTTGATTATAATTTTCTGGTCTGTCAACTCTCTCTGAGTCTCTTTTGTAGACTTGTAATCTGCAATATCTTGACTTTGCTGTGAATGATTTTACATGACGTTTACTGACTGCATAAAATTGTTCTTTTAGTCTCTAGAATTGAAGCATGGAGTGAGAAGCTTTCCATGTATATGCTGCTGGATGTAAATACAGAGATATATCCAATTGATGAAGGCGACAGGATTCTGATGGTCCTGTCTCCCACGCTTAATTATGATGGAGCTCCTGTCACCAGCTATAGTGGTCAGGTAATATTTTCCCCCTTAATGTTTAATTGAATTTCGTACCATGCTGGAGCTCGTGTCATTTGTTTCATGTTTCGACAGATACACTTTTCTGCATTTATATGCATCTTAAAGATTCTAAAAGAAACAACTAATAATTCCATAAACTTTATCTGATACTGAAAATTTGGAGAAGGATTTTACCGAACAGTCCCAAAAAAATTAGATAAGAGGGTCACTGAACTGCAAGGCAAGTATCCATTGGTTCTGTTCTGTTAAGGTTGTGCTAGCCATGTCTCTTAAAAAGAAATGAGAGAAAAGGAAAGGTCATTTAAGTAGGTGAGATAAGGCTTGTGTCCAAGGCTTGTGACGGATGGTATAGACTTGTTTAATACTGAAGACACTCGCCTTATGCTCCTTGAAAATATGGAGGAGTATTTTCTCAGTCTCAACAGAACTCTTCAAGATGAGCGTCTTTGTAGGATCAATTTGTTGGAGGAGTTCCTTGGTAGTCTCCCGGGAAAGGGGTCTGGTTCTCTTGCCATCGGTTTTTTGCTGCCTCGTTGCATTCATGGCTCAAAAGAAATTGGTGAGTCTTTAGGGATATAGAGAAGTCATGAAGAGGAGGTTTGGACTCTTTGGAGTGAGTTGCATTTCAATGCCTCTCTCTAGGTGTGCTTGTTTAACTTTGGGAACAAACTGCTGTAATTTTCCTTTTGTTATGTTTTGTAAGCTACTTTGTCTGGCCCATTCTTTTTCGTCATCCGCTCCTTTTGAGAAAACATATATATTCTCTTAGGAAGAAAATATATATGTTTTCTTTTTATAATTTGACTAGGTTTCTATAAACTTTCCATAAAGTATTAAAAAAAAAAGAACAGTGATTATAAGCAAACAACTTTATGTTCTAGACGCAAAACTAAAAACAGAATTCTGCAAATGTTCCTTAGTATGTGAAGTGCCGAGGATGTGCTAACAAGAATCGCTTTGACAAACCTGTTTTACTTCAGCTGTCAAGAATGTGAACATAATTTAAATCTCTAGTAATAGTAATATCTTTCAATTGAACTTAATGTTTTTCAGGAAGGGAAGAAATCACTAGCAGATAAATTTGAGTATATCATGCATGGGAAACTGTATAAACTTTCAGACGAAGGCTCAGGATCAGACTTGAAAGTGTAAGTGAACTTGCATTACCATGATTTTCCTCAACGATTTGAATCTGCTATTTTTTTTTTGGTTTGGGGGTTCAAATATCCTAAATGTATAACTTAGTCCCTTATTTGTGCCTTTGAACAAAATGCTAAAATAAGAAAGAATGATCATTTATAAATTTGGAAAAGATATTTTTTAAGTGGTTGATGTTAGACTATGTTGATATAATTAAATTTACCCCAAACTCTCAGCTTAAGCTTTTAGTTTGATTGGTGATTTAAGATGGTATCAGAGTGGGTGGTCCAGGAGATCCTGTGTTCAAGCCTTGCAAAGTCAGTTTCCTCCCCATTTAATCTTGATTTACCCCAAACTCTCAGCTTAAGCTTTTAGTTTGATTGGTGATTTAAGAGTTTACTCACCACTATCTGCCCATTTTGGTGGATACATTTTGTATAACATCAAACCATTTTTGGGTTAGATAAGTGTAAGGTGAACTCCCTATACTATATGGTTTGATATTCAAAATGAAAACAAGCAAGAATAAAACAAGTGCCCTACGAATTTGAGAGGGTGTGAGAGTACTAACAGGAGATATGTTGGAAATATTTGTAGTGTAGTAATAAAGACATAGTGGTAATTAGTTAGGAGTGACTTGGTTATAAATAGAGGGAGTTTAGGGGCCTTAGAAGGGCGATGGTTTTGTGTATTGTCTAGTAAGGAAACAAACACAATTTTTAAGTTATCAAATGGGGCATTATAATTCTAACCTTTCTTAAAGTGTAAGTATTTTTTTTTAATATGTCATCTCATAAAAAATATAGTATATTTTCAACTCAATTAATAAAATACATTTTAATTCTGATGTTGTTTGGTTGGAATGGTTGTATTTGCAGAGAGGTTTATGCATCTTTTGGAGGACTCCAAATGATGTTGAAGGGAGATCCCCTCCACTGTGCCAAATTCAGGATGGATCAGAGCATGTTTTTGCTCCTCCGAAAAATCACATGAACTAATTATGGCCTTTTTTTATTACAGAAATTGTAATAACTCTGTTTTTTTTTTTTTTTTACTTATAGTTAATTTATGAATTATGAAAACTTTCACTAAATTGATCTTCATAGTTTCTGTATGGTTTAACATACATGGGTAAATTTTATTTGAAATTCTTTTTTCTTTCCCTTCTAAAAAA

mRNA sequence

AAACCCTAGTCCCTCACCGTTAAAAAAAAAAAAACCCTATACCCCTCATCTTTCATTTTCTACCCCCTTCTTCCCGCCGGCTCTTCCTTTCTTTCAGCAGCTCACTGATTCCGCCGCCGTCGTTCTCCTGCATGTTCCTGCGCCTCCTCCGTCATAGCGGTTTGCACTGATTTTCTACTCTCTTGTTGTTATTGGTATTCTCCCAAATTCCATCCATGTCTTTCTTCTCACTTGAAAAACTTAAATTAGGACTTAGTCTAGTGAAAGAATAGAGTTGTTTTTTTTTTTTTGGTCTCGGCTGAAAATTTCATTGTTTCTAGTCTCCCACATCTTCCAAAGCATCCCCCACTGTGAATTTTTGAGCATCTCCCTCCTTTATTTGTCTCGAATCAGTCGAAGTAATCTATCACACACCAATTTTCCCTATTAAACAAAAATTCCCAGATATTAGAATTAAAGTTGTTATAACCAAATTTCTTTTCGAAACTTACACCGCATGGCAGATTTGCATCTTGAGGAGTAAATTCTCCATAAACATGGTGGAGCTTCTATTTGATGATATCTTCAAGGTTAAGTGTGTCAACCCAGATGGTAAAAAGTATGATAAAGTCTCTAGAATTGAAGCATGGAGTGAGAAGCTTTCCATGTATATGCTGCTGGATGTAAATACAGAGATATATCCAATTGATGAAGGCGACAGGATTCTGATGGTCCTGTCTCCCACGCTTAATTATGATGGAGCTCCTGTCACCAGCTATAGTGGTCAGGAAGGGAAGAAATCACTAGCAGATAAATTTGAGTATATCATGCATGGGAAACTGTATAAACTTTCAGACGAAGGCTCAGGATCAGACTTGAAAGTAGAGGTTTATGCATCTTTTGGAGGACTCCAAATGATGTTGAAGGGAGATCCCCTCCACTGTGCCAAATTCAGGATGGATCAGAGCATGTTTTTGCTCCTCCGAAAAATCACATGAACTAATTATGGCCTTTTTTTATTACAGAAATTGTAATAACTCTGTTTTTTTTTTTTTTTTACTTATAGTTAATTTATGAATTATGAAAACTTTCACTAAATTGATCTTCATAGTTTCTGTATGGTTTAACATACATGGGTAAATTTTATTTGAAATTCTTTTTTCTTTCCCTTCTAAAAAA

Coding sequence (CDS)

ATGGTGGAGCTTCTATTTGATGATATCTTCAAGGTTAAGTGTGTCAACCCAGATGGTAAAAAGTATGATAAAGTCTCTAGAATTGAAGCATGGAGTGAGAAGCTTTCCATGTATATGCTGCTGGATGTAAATACAGAGATATATCCAATTGATGAAGGCGACAGGATTCTGATGGTCCTGTCTCCCACGCTTAATTATGATGGAGCTCCTGTCACCAGCTATAGTGGTCAGGAAGGGAAGAAATCACTAGCAGATAAATTTGAGTATATCATGCATGGGAAACTGTATAAACTTTCAGACGAAGGCTCAGGATCAGACTTGAAAGTAGAGGTTTATGCATCTTTTGGAGGACTCCAAATGATGTTGAAGGGAGATCCCCTCCACTGTGCCAAATTCAGGATGGATCAGAGCATGTTTTTGCTCCTCCGAAAAATCACATGA

Protein sequence

MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVLSPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQMMLKGDPLHCAKFRMDQSMFLLLRKIT

Homology

[Close]

BLAST of Tan0000631.3 vs. ExPASy Swiss-Prot
Match: O81097 (DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana OX=3702 GN=NRPB8A PE=1 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 1.2e-42
Identity = 82/142 (57.75%), Postives = 106/142 (74.65%), Query Frame = 0


Query: 4   LLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVLSPT 63
           +LF+DIF V  ++PDGKK+DKV+R++A S  L M+M LDVNTE+YP+  GD+  + L+PT
Sbjct: 6   ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPT 65

Query: 64  LNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQMMLK 123
           LN DG P T Y     KK+LADK+EYIMHGKLYK+S E  G   K E+Y SFGGL M+LK
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKIS-ERDGKTPKAELYVSFGGLLMLLK 125

Query: 124 GDPLHCAKFRMDQSMFLLLRKI 146
           GDP H + F +DQ +FLL+RK+
Sbjct: 126 GDPAHISHFELDQRLFLLMRKL 146

[Close]

BLAST of Tan0000631.3 vs. ExPASy Swiss-Prot
Match: Q9M1A8 (DNA-directed RNA polymerases II, IV and V subunit 8B OS=Arabidopsis thaliana OX=3702 GN=NRPB8B PE=1 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 5.8e-42
Identity = 80/142 (56.34%), Postives = 106/142 (74.65%), Query Frame = 0


Query: 4   LLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVLSPT 63
           ++F+DIF V  ++PDGKK+DKV+R+EA S  L M+M LDVNTE+YP+  GD+  + ++PT
Sbjct: 6   IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMAPT 65

Query: 64  LNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQMMLK 123
           LN DG P T Y     KK+LADK+EYIMHGKLYK+S E  G   K E+Y SFGGL M+L+
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKIS-ERDGKTPKAELYVSFGGLLMLLQ 125

Query: 124 GDPLHCAKFRMDQSMFLLLRKI 146
           GDP H + F +DQ +FLL+RK+
Sbjct: 126 GDPAHISHFELDQRLFLLMRKL 146

[Close]

BLAST of Tan0000631.3 vs. ExPASy Swiss-Prot
Match: Q54YW8 (DNA-directed RNA polymerases I, II, and III subunit rpabc3 OS=Dictyostelium discoideum OX=44689 GN=polr2h PE=3 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 6.6e-30
Identity = 65/144 (45.14%), Postives = 97/144 (67.36%), Query Frame = 0


Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M  +LF+D F+VK ++PDGKK+D+VSR   +SE   M + +D+ T IYP+   +R  MVL
Sbjct: 1   MTSILFEDTFEVKEIDPDGKKFDRVSRFVCYSENYEMDLQIDIATHIYPLGH-ERFKMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           + +LN DG+   S S    K SLADK++Y+M+GK++K   E S S  KV V+ASFGGL +
Sbjct: 61  ASSLNLDGSSDNSGSYDTNKPSLADKYDYVMYGKIFKYQKENSSS--KVSVFASFGGLLV 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRK 145
           +L+GDP +     +D  ++LL++K
Sbjct: 121 LLQGDPRYLPGIELDARIYLLIKK 141

[Close]

BLAST of Tan0000631.3 vs. ExPASy Swiss-Prot
Match: Q19826 (Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhabditis elegans OX=6239 GN=rpb-8 PE=1 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 1.9e-29
Identity = 59/145 (40.69%), Postives = 99/145 (68.28%), Query Frame = 0


Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M  ++FDD+FKVK V+PDGKK+D+VSR    +E   M +++D+N++IYP+ + D++ +VL
Sbjct: 1   MAGIIFDDMFKVKSVDPDGKKFDRVSRYFCDAESFKMELIIDINSQIYPLKQNDKVRLVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSD-LKVEVYASFGGLQ 120
           + TL  DG         + +     ++EY+M+GK+Y+L D+ +G+D  K+  YASFGGL 
Sbjct: 61  ATTLREDGLADEGEYDPKAEYPRIKQYEYVMYGKVYRLEDDDTGTDGGKLAAYASFGGLL 120

Query: 121 MMLKGDPLHCAKFRMDQSMFLLLRK 145
           M LKG+ ++   F +D +++LL++K
Sbjct: 121 MRLKGEAINLHGFEVDMNLYLLMKK 145

[Close]

BLAST of Tan0000631.3 vs. ExPASy Swiss-Prot
Match: A8XT81 (Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhabditis briggsae OX=6238 GN=rpb-8 PE=3 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 2.1e-28
Identity = 58/145 (40.00%), Postives = 97/145 (66.90%), Query Frame = 0


Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M  ++FDD+F VK V+PDGKK+D+VSR    +E   M +++D+N++IYP+ + D+  +VL
Sbjct: 1   MAGIIFDDMFSVKSVDPDGKKFDRVSRYFCDAESFKMELIIDINSQIYPLKQNDKFRLVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSD-LKVEVYASFGGLQ 120
           + TL  DG         + +     ++EY+M+GK+Y+L D+ SG+D  ++  YASFGGL 
Sbjct: 61  ATTLREDGLADEGEYDPKAEYPRIKQYEYVMYGKVYRLEDDDSGTDGGQLAAYASFGGLL 120

Query: 121 MMLKGDPLHCAKFRMDQSMFLLLRK 145
           M LKG+ ++   F +D +++LL++K
Sbjct: 121 MRLKGEAINLHGFEVDMNLYLLMKK 145

[Close]

BLAST of Tan0000631.3 vs. NCBI nr
Match: XP_022983196.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983203.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983212.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983221.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983229.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima])

HSP 1 Score: 285.4 bits (729), Expect = 2.7e-73
Identity = 138/146 (94.52%), Postives = 144/146 (98.63%), Query Frame = 0


Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M E+LFDDIFKVKCVNPDGKKYDKVSRIEA SEKLSMYMLLDVNTEIYPIDEGD+ILMVL
Sbjct: 1   MAEVLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKL+DEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRKIT 147
           MLKGDPLHCAKFR+DQ+MFLLLRK+T
Sbjct: 121 MLKGDPLHCAKFRVDQNMFLLLRKLT 146

[Close]

BLAST of Tan0000631.3 vs. NCBI nr
Match: KAG6600626.1 (DNA-directed RNA polymerases II, IV and V subunit 8B, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031262.1 DNA-directed RNA polymerases II, IV and V subunit 8B, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 283.5 bits (724), Expect = 1.0e-72
Identity = 137/146 (93.84%), Postives = 143/146 (97.95%), Query Frame = 0


Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M ELLFDDIFKVKCVNPDGKKYDKVSRIEA SEKLSMYMLLDVNTEIYPIDEGD+ILMVL
Sbjct: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVT YSGQ+GKKSLADKFEYIMHGKLYKL+DEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRKIT 147
           MLKGDPLHCAKFR+DQ+MFLLLRK+T
Sbjct: 121 MLKGDPLHCAKFRVDQNMFLLLRKLT 146

[Close]

BLAST of Tan0000631.3 vs. NCBI nr
Match: XP_022942165.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata])

HSP 1 Score: 279.6 bits (714), Expect = 1.5e-71
Identity = 136/145 (93.79%), Postives = 141/145 (97.24%), Query Frame = 0


Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M ELLFDDIFKVKCVNPDGKKYDKVSRIEA SEKLSMYMLLDVNTEIYPIDEGD+ILMVL
Sbjct: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMH KLYKL+DEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHRKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRKI 146
           MLKGDPLHC KFR+DQ+MFLLLRK+
Sbjct: 121 MLKGDPLHCVKFRVDQNMFLLLRKL 145

[Close]

BLAST of Tan0000631.3 vs. NCBI nr
Match: XP_022929200.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata] >XP_022929202.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata] >XP_022985172.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_023551974.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita pepo subsp. pepo] >KAG6577274.1 DNA-directed RNA polymerases II, IV and V subunit 8B, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015358.1 DNA-directed RNA polymerases II, IV and V subunit 8B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 278.9 bits (712), Expect = 2.6e-71
Identity = 133/145 (91.72%), Postives = 143/145 (98.62%), Query Frame = 0


Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M ELLF+D+FKVKCVNPDGKKYDKVSRIEA SEKLSMYMLLDVNTEIYPIDEG++ILMVL
Sbjct: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGK+YKLSDEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRKI 146
           +LKGDP+HCAKFR+DQ+MFLLLRK+
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 145

[Close]

BLAST of Tan0000631.3 vs. NCBI nr
Match: XP_038901286.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like [Benincasa hispida])

HSP 1 Score: 275.8 bits (704), Expect = 2.2e-70
Identity = 132/144 (91.67%), Postives = 141/144 (97.92%), Query Frame = 0


Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M ELLFDDIF VKCVNPDGKKYDKVSRIEA SEKLSMYMLLDVNTEIYPIDEG++ILMVL
Sbjct: 1   MAELLFDDIFMVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSY+GQEGKKSLADKFEYIMHGK+YKLSD+GSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYTGQEGKKSLADKFEYIMHGKIYKLSDDGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRK 145
           +LKGDP+HCAKFR+DQ+MFLLLRK
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRK 144

[Close]

BLAST of Tan0000631.3 vs. ExPASy TrEMBL
Match: A0A6J1J6Q9 (DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita maxima OX=3661 GN=LOC111481850 PE=3 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 1.3e-73
Identity = 138/146 (94.52%), Postives = 144/146 (98.63%), Query Frame = 0


Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M E+LFDDIFKVKCVNPDGKKYDKVSRIEA SEKLSMYMLLDVNTEIYPIDEGD+ILMVL
Sbjct: 1   MAEVLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKL+DEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRKIT 147
           MLKGDPLHCAKFR+DQ+MFLLLRK+T
Sbjct: 121 MLKGDPLHCAKFRVDQNMFLLLRKLT 146

[Close]

BLAST of Tan0000631.3 vs. ExPASy TrEMBL
Match: A0A6J1FVR2 (DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita moschata OX=3662 GN=LOC111447307 PE=3 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 7.3e-72
Identity = 136/145 (93.79%), Postives = 141/145 (97.24%), Query Frame = 0


Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M ELLFDDIFKVKCVNPDGKKYDKVSRIEA SEKLSMYMLLDVNTEIYPIDEGD+ILMVL
Sbjct: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMH KLYKL+DEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHRKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRKI 146
           MLKGDPLHC KFR+DQ+MFLLLRK+
Sbjct: 121 MLKGDPLHCVKFRVDQNMFLLLRKL 145

[Close]

BLAST of Tan0000631.3 vs. ExPASy TrEMBL
Match: A0A6J1ETP8 (DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita moschata OX=3662 GN=LOC111435865 PE=3 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.2e-71
Identity = 133/145 (91.72%), Postives = 143/145 (98.62%), Query Frame = 0


Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M ELLF+D+FKVKCVNPDGKKYDKVSRIEA SEKLSMYMLLDVNTEIYPIDEG++ILMVL
Sbjct: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGK+YKLSDEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRKI 146
           +LKGDP+HCAKFR+DQ+MFLLLRK+
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 145

[Close]

BLAST of Tan0000631.3 vs. ExPASy TrEMBL
Match: A0A6J1J454 (DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita maxima OX=3661 GN=LOC111483257 PE=3 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.2e-71
Identity = 133/145 (91.72%), Postives = 143/145 (98.62%), Query Frame = 0


Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M ELLF+D+FKVKCVNPDGKKYDKVSRIEA SEKLSMYMLLDVNTEIYPIDEG++ILMVL
Sbjct: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGK+YKLSDEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRKI 146
           +LKGDP+HCAKFR+DQ+MFLLLRK+
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 145

[Close]

BLAST of Tan0000631.3 vs. ExPASy TrEMBL
Match: A0A6J1C3H4 (DNA-directed RNA polymerases II and V subunit 8A-like OS=Momordica charantia OX=3673 GN=LOC111008140 PE=3 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 4.4e-69
Identity = 129/145 (88.97%), Postives = 142/145 (97.93%), Query Frame = 0


Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           MVELLFDDIFKVKCVNPDG+KYDKVSRI+A SEKL+M+MLLDVNTEIYPIDEG++ILMVL
Sbjct: 1   MVELLFDDIFKVKCVNPDGQKYDKVSRIDARSEKLAMHMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPV S+S QEGKKSLADKFEY+MHGKLYKLS+EGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVASHSSQEGKKSLADKFEYVMHGKLYKLSEEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRKI 146
           MLKGDP+HCAKFR+DQ+MFLLLRK+
Sbjct: 121 MLKGDPVHCAKFRVDQNMFLLLRKL 145

[Close]

BLAST of Tan0000631.3 vs. TAIR 10
Match: AT1G54250.1 (RNA polymerase Rpb8 )

HSP 1 Score: 174.1 bits (440), Expect = 8.3e-44
Identity = 82/142 (57.75%), Postives = 106/142 (74.65%), Query Frame = 0


Query: 4   LLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVLSPT 63
           +LF+DIF V  ++PDGKK+DKV+R++A S  L M+M LDVNTE+YP+  GD+  + L+PT
Sbjct: 6   ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPT 65

Query: 64  LNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQMMLK 123
           LN DG P T Y     KK+LADK+EYIMHGKLYK+S E  G   K E+Y SFGGL M+LK
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKIS-ERDGKTPKAELYVSFGGLLMLLK 125

Query: 124 GDPLHCAKFRMDQSMFLLLRKI 146
           GDP H + F +DQ +FLL+RK+
Sbjct: 126 GDPAHISHFELDQRLFLLMRKL 146

[Close]

BLAST of Tan0000631.3 vs. TAIR 10
Match: AT3G59600.1 (RNA polymerase Rpb8 )

HSP 1 Score: 171.8 bits (434), Expect = 4.1e-43
Identity = 80/142 (56.34%), Postives = 106/142 (74.65%), Query Frame = 0


Query: 4   LLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVLSPT 63
           ++F+DIF V  ++PDGKK+DKV+R+EA S  L M+M LDVNTE+YP+  GD+  + ++PT
Sbjct: 6   IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMAPT 65

Query: 64  LNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQMMLK 123
           LN DG P T Y     KK+LADK+EYIMHGKLYK+S E  G   K E+Y SFGGL M+L+
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKIS-ERDGKTPKAELYVSFGGLLMLLQ 125

Query: 124 GDPLHCAKFRMDQSMFLLLRKI 146
           GDP H + F +DQ +FLL+RK+
Sbjct: 126 GDPAHISHFELDQRLFLLMRKL 146

The following BLAST results are available for this feature:

BLAST of Tan0000631.3 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81097	1.2e-42	57.75	DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana OX=3702...	[more]
Q9M1A8	5.8e-42	56.34	DNA-directed RNA polymerases II, IV and V subunit 8B OS=Arabidopsis thaliana OX=...	[more]
Q54YW8	6.6e-30	45.14	DNA-directed RNA polymerases I, II, and III subunit rpabc3 OS=Dictyostelium disc...	[more]
Q19826	1.9e-29	40.69	Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhab...	[more]
A8XT81	2.1e-28	40.00	Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhab...	[more]

BLAST of Tan0000631.3 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022983196.1	2.7e-73	94.52	DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP...	[more]
KAG6600626.1	1.0e-72	93.84	DNA-directed RNA polymerases II, IV and V subunit 8B, partial [Cucurbita argyros...	[more]
XP_022942165.1	1.5e-71	93.79	DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata]	[more]
XP_022929200.1	2.6e-71	91.72	DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata] >...	[more]
XP_038901286.1	2.2e-70	91.67	DNA-directed RNA polymerases II, IV and V subunit 8B-like [Benincasa hispida]	[more]

BLAST of Tan0000631.3 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J6Q9	1.3e-73	94.52	DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita maxima OX...	[more]
A0A6J1FVR2	7.3e-72	93.79	DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita moschata ...	[more]
A0A6J1ETP8	1.2e-71	91.72	DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita moschata ...	[more]
A0A6J1J454	1.2e-71	91.72	DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita maxima OX...	[more]
A0A6J1C3H4	4.4e-69	88.97	DNA-directed RNA polymerases II and V subunit 8A-like OS=Momordica charantia OX=...	[more]

BLAST of Tan0000631.3 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G54250.1	8.3e-44	57.75	RNA polymerase Rpb8	[more]
AT3G59600.1	4.1e-43	56.34	RNA polymerase Rpb8	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005570	RNA polymerase, Rpb8	SMART	SM00658	rpol8neu	coord: 2..144 e-value: 6.1E-56 score: 201.8
IPR005570	RNA polymerase, Rpb8	PFAM	PF03870	RNA_pol_Rpb8	coord: 7..144 e-value: 6.7E-49 score: 165.6
IPR005570	RNA polymerase, Rpb8	PIRSF	PIRSF000779	RPB8	coord: 1..146 e-value: 1.2E-46 score: 156.4
IPR005570	RNA polymerase, Rpb8	PANTHER	PTHR10917	DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3	coord: 1..145
None	No IPR available	GENE3D	2.40.50.140		coord: 6..146 e-value: 5.3E-53 score: 180.9
None	No IPR available	PANTHER	PTHR10917:SF1	DNA-DIRECTED RNA POLYMERASE I, II	coord: 1..145
IPR012340	Nucleic acid-binding, OB-fold	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 4..144

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Tan0000631	Tan0000631	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0000631.3-five_prime_utr	Tan0000631.3-five_prime_utr-LG01:13183831..13184333	five_prime_UTR
Tan0000631.3-five_prime_utr	Tan0000631.3-five_prime_utr-LG01:13184625..13184657	five_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0000631.3-exon	Tan0000631.3-exon-LG01:13183831..13184333	exon
Tan0000631.3-exon	Tan0000631.3-exon-LG01:13184625..13184730	exon
Tan0000631.3-exon	Tan0000631.3-exon-LG01:13184898..13185055	exon
Tan0000631.3-exon	Tan0000631.3-exon-LG01:13186115..13186209	exon
Tan0000631.3-exon	Tan0000631.3-exon-LG01:13187080..13187375	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0000631.3-cds	Tan0000631.3-cds-LG01:13184658..13184730	CDS
Tan0000631.3-cds	Tan0000631.3-cds-LG01:13184898..13185055	CDS
Tan0000631.3-cds	Tan0000631.3-cds-LG01:13186115..13186209	CDS
Tan0000631.3-cds	Tan0000631.3-cds-LG01:13187080..13187194	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Tan0000631.3-three_prime_utr	Tan0000631.3-three_prime_utr-LG01:13187195..13187375	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Tan0000631.3	Tan0000631.3-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0005736	RNA polymerase I complex
cellular_component	GO:0005665	RNA polymerase II, core complex
cellular_component	GO:0005666	RNA polymerase III complex
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity