Tan0000631 (gene) Snake gourd v1

Overview


Name	Tan0000631
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	DNA-directed RNA polymerases II and V subunit 8A-like
Location	LG01: 13183831 .. 13187375 (+)
RNA-Seq Expression	Tan0000631
Synteny	Tan0000631

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAACCCTAGTCCCTCACCGTTAAAAAAAAAAAAACCCTATACCCCTCATCTTTCATTTTCTACCCCCTTCTTCCCGCCGGCTCTTCCTTTCTTTCAGCAGCTCACTGATTCCGCCGCCGTCGTTCTCCTGCATGTTCCTGCGCCTCCTCCGTCATAGCGGTTTGCACTGATTTTCTACTCTCTTGTTGTTATTGGTATTCTCCCAAATTCCATCCATGTCTTTCTTCTCACTTGAAAAACTTAAATTAGGACTTAGTCTAGTGAAAGAATAGAGTTGTTTTTTTTTTTTTGGTCTCGGCTGAAAATTTCATTGTTTCTAGTCTCCCACATCTTCCAAAGCATCCCCCACTGTGAATTTTTGAGCATCTCCCTCCTTTATTTGTCTCGAATCAGTCGAAGTAATCTATCACACACCAATTTTCCCTATTAAACAAAAATTCCCAGATATTAGAATTAAAGTTGTTATAACCAAATTTCTTTTCGAAACTTACACCGCATGGCAGGTAGTTTCGATTTTACTCTTTTTGTTTTTAGCGTCAACTTGTTCAATACAATCTGGTCCTGGACAGACTTGGAAGTTCTTGTATTGGTTGTTTTCTGGGGTTTGAACGTTGGAATTTGCTTTATCAATTTTCTACTGTAATTTTGCGTTTGCGCTGAGTGCTTTTTTTAATTCCCAATTCAAATACTTTCTCCAACCCCTCTACTATTTCATTCTGATTCTGCTCAAATTCTAGTTGGCGCTCTTTTTCCCTGCTTTCTATTTATTATTTGTTGCTTGCCAACCGACTCAGATTTGCATCTTGAGGAGTAAATTCTCCATAAACATGGTGGAGCTTCTATTTGATGATATCTTCAAGGTTAAGTGTGTCAACCCAGATGGTAAAAAGTATGATAAAGGTACTTGCCCTCAGTCTTGTTACTGCTCTCGCATTCTTTTGATTATAATTTTCTGGTCTGTCAACTCTCTCTGAGTCTCTTTTGTAGACTTGTAATCTGCAATATCTTGACTTTGCTGTGAATGATTTTACATGACGTTTACTGACTGCATAAAATTGTTCTTTTAGTCTCTAGAATTGAAGCATGGAGTGAGAAGCTTTCCATGTATATGCTGCTGGATGTAAATACAGAGATATATCCAATTGATGAAGGCGACAGGATTCTGATGGTCCTGTCTCCCACGCTTAATTATGATGGAGCTCCTGTCACCAGCTATAGTGGTCAGGTAATATTTTCCCCCTTAATGTTTAATTGAATTTCGTACCATGCTGGAGCTCGTGTCATTTGTTTCATGTTTCGACAGATACACTTTTCTGCATTTATATGCATCTTAAAGATTCTAAAAGAAACAACTAATAATTCCATAAACTTTATCTGATACTGAAAATTTGGAGAAGGATTTTACCGAACAGTCCCAAAAAAATTAGATAAGAGGGTCACTGAACTGCAAGGCAAGTATCCATTGGTTCTGTTCTGTTAAGGTTGTGCTAGCCATGTCTCTTAAAAAGAAATGAGAGAAAAGGAAAGGTCATTTAAGTAGGTGAGATAAGGCTTGTGTCCAAGGCTTGTGACGGATGGTATAGACTTGTTTAATACTGAAGACACTCGCCTTATGCTCCTTGAAAATATGGAGGAGTATTTTCTCAGTCTCAACAGAACTCTTCAAGATGAGCGTCTTTGTAGGATCAATTTGTTGGAGGAGTTCCTTGGTAGTCTCCCGGGAAAGGGGTCTGGTTCTCTTGCCATCGGTTTTTTGCTGCCTCGTTGCATTCATGGCTCAAAAGAAATTGGTGAGTCTTTAGGGATATAGAGAAGTCATGAAGAGGAGGTTTGGACTCTTTGGAGTGAGTTGCATTTCAATGCCTCTCTCTAGGTGTGCTTGTTTAACTTTGGGAACAAACTGCTGTAATTTTCCTTTTGTTATGTTTTGTAAGCTACTTTGTCTGGCCCATTCTTTTTCGTCATCCGCTCCTTTTGAGAAAACATATATATTCTCTTAGGAAGAAAATATATATGTTTTCTTTTTATAATTTGACTAGGTTTCTATAAACTTTCCATAAAGTATTAAAAAAAAAAGAACAGTGATTATAAGCAAACAACTTTATGTTCTAGACGCAAAACTAAAAACAGAATTCTGCAAATGTTCCTTAGTATGTGAAGTGCCGAGGATGTGCTAACAAGAATCGCTTTGACAAACCTGTTTTACTTCAGCTGTCAAGAATGTGAACATAATTTAAATCTCTAGTAATAGTAATATCTTTCAATTGAACTTAATGTTTTTCAGGAAGGGAAGAAATCACTAGCAGATAAATTTGAGTATATCATGCATGGGAAACTGTATAAACTTTCAGACGAAGGCTCAGGATCAGACTTGAAAGTGTAAGTGAACTTGCATTACCATGATTTTCCTCAACGATTTGAATCTGCTATTTTTTTTTTGGTTTGGGGGTTCAAATATCCTAAATGTATAACTTAGTCCCTTATTTGTGCCTTTGAACAAAATGCTAAAATAAGAAAGAATGATCATTTATAAATTTGGAAAAGATATTTTTTAAGTGGTTGATGTTAGACTATGTTGATATAATTAAATTTACCCCAAACTCTCAGCTTAAGCTTTTAGTTTGATTGGTGATTTAAGATGGTATCAGAGTGGGTGGTCCAGGAGATCCTGTGTTCAAGCCTTGCAAAGTCAGTTTCCTCCCCATTTAATCTTGATTTACCCCAAACTCTCAGCTTAAGCTTTTAGTTTGATTGGTGATTTAAGAGTTTACTCACCACTATCTGCCCATTTTGGTGGATACATTTTGTATAACATCAAACCATTTTTGGGTTAGATAAGTGTAAGGTGAACTCCCTATACTATATGGTTTGATATTCAAAATGAAAACAAGCAAGAATAAAACAAGTGCCCTACGAATTTGAGAGGGTGTGAGAGTACTAACAGGAGATATGTTGGAAATATTTGTAGTGTAGTAATAAAGACATAGTGGTAATTAGTTAGGAGTGACTTGGTTATAAATAGAGGGAGTTTAGGGGCCTTAGAAGGGCGATGGTTTTGTGTATTGTCTAGTAAGGAAACAAACACAATTTTTAAGTTATCAAATGGGGCATTATAATTCTAACCTTTCTTAAAGTGTAAGTATTTTTTTTTAATATGTCATCTCATAAAAAATATAGTATATTTTCAACTCAATTAATAAAATACATTTTAATTCTGATGTTGTTTGGTTGGAATGGTTGTATTTGCAGAGAGGTTTATGCATCTTTTGGAGGACTCCAAATGATGTTGAAGGGAGATCCCCTCCACTGTGCCAAATTCAGGATGGATCAGAGCATGTTTTTGCTCCTCCGAAAAATCACATGAACTAATTATGGCCTTTTTTTATTACAGAAATTGTAATAACTCTGTTTTTTTTTTTTTTTTACTTATAGTTAATTTATGAATTATGAAAACTTTCACTAAATTGATCTTCATAGTTTCTGTATGGTTTAACATACATGGGTAAATTTTATTTGAAATTCTTTTTTCTTTCCCTTCTAAAAAA

mRNA sequence

AAACCCTAGTCCCTCACCGTTAAAAAAAAAAAAACCCTATACCCCTCATCTTTCATTTTCTACCCCCTTCTTCCCGCCGGCTCTTCCTTTCTTTCAGCAGCTCACTGATTCCGCCGCCGTCGTTCTCCTGCATGTTCCTGCGCCTCCTCCGTCATAGCGATTTGCATCTTGAGGAGTAAATTCTCCATAAACATGGTGGAGCTTCTATTTGATGATATCTTCAAGGTTAAGTGTGTCAACCCAGATGGTAAAAAGTATGATAAAGTCTCTAGAATTGAAGCATGGAGTGAGAAGCTTTCCATGTATATGCTGCTGGATGTAAATACAGAGATATATCCAATTGATGAAGGCGACAGGATTCTGATGGTCCTGTCTCCCACGCTTAATTATGATGGAGCTCCTGTCACCAGCTATAGTGGTCAGGAAGGGAAGAAATCACTAGCAGATAAATTTGAGTATATCATGCATGGGAAACTGTATAAACTTTCAGACGAAGGCTCAGGATCAGACTTGAAAGTAGAGGTTTATGCATCTTTTGGAGGACTCCAAATGATGTTGAAGGGAGATCCCCTCCACTGTGCCAAATTCAGGATGGATCAGAGCATGTTTTTGCTCCTCCGAAAAATCACATGAACTAATTATGGCCTTTTTTTATTACAGAAATTGTAATAACTCTGTTTTTTTTTTTTTTTTACTTATAGTTAATTTATGAATTATGAAAACTTTCACTAAATTGATCTTCATAGTTTCTGTATGGTTTAACATACATGGGTAAATTTTATTTGAAATTCTTTTTTCTTTCCCTTCTAAAAAA

Coding sequence (CDS)

ATGGTGGAGCTTCTATTTGATGATATCTTCAAGGTTAAGTGTGTCAACCCAGATGGTAAAAAGTATGATAAAGTCTCTAGAATTGAAGCATGGAGTGAGAAGCTTTCCATGTATATGCTGCTGGATGTAAATACAGAGATATATCCAATTGATGAAGGCGACAGGATTCTGATGGTCCTGTCTCCCACGCTTAATTATGATGGAGCTCCTGTCACCAGCTATAGTGGTCAGGAAGGGAAGAAATCACTAGCAGATAAATTTGAGTATATCATGCATGGGAAACTGTATAAACTTTCAGACGAAGGCTCAGGATCAGACTTGAAAGTAGAGGTTTATGCATCTTTTGGAGGACTCCAAATGATGTTGAAGGGAGATCCCCTCCACTGTGCCAAATTCAGGATGGATCAGAGCATGTTTTTGCTCCTCCGAAAAATCACATGA

Protein sequence

MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVLSPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQMMLKGDPLHCAKFRMDQSMFLLLRKIT

Homology

[Close]

BLAST of Tan0000631 vs. ExPASy Swiss-Prot
Match: O81097 (DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana OX=3702 GN=NRPB8A PE=1 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 1.2e-42
Identity = 82/142 (57.75%), Postives = 106/142 (74.65%), Query Frame = 0


Query: 4   LLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVLSPT 63
           +LF+DIF V  ++PDGKK+DKV+R++A S  L M+M LDVNTE+YP+  GD+  + L+PT
Sbjct: 6   ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPT 65

Query: 64  LNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQMMLK 123
           LN DG P T Y     KK+LADK+EYIMHGKLYK+S E  G   K E+Y SFGGL M+LK
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKIS-ERDGKTPKAELYVSFGGLLMLLK 125

Query: 124 GDPLHCAKFRMDQSMFLLLRKI 146
           GDP H + F +DQ +FLL+RK+
Sbjct: 126 GDPAHISHFELDQRLFLLMRKL 146

[Close]

BLAST of Tan0000631 vs. ExPASy Swiss-Prot
Match: Q9M1A8 (DNA-directed RNA polymerases II, IV and V subunit 8B OS=Arabidopsis thaliana OX=3702 GN=NRPB8B PE=1 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 5.8e-42
Identity = 80/142 (56.34%), Postives = 106/142 (74.65%), Query Frame = 0


Query: 4   LLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVLSPT 63
           ++F+DIF V  ++PDGKK+DKV+R+EA S  L M+M LDVNTE+YP+  GD+  + ++PT
Sbjct: 6   IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMAPT 65

Query: 64  LNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQMMLK 123
           LN DG P T Y     KK+LADK+EYIMHGKLYK+S E  G   K E+Y SFGGL M+L+
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKIS-ERDGKTPKAELYVSFGGLLMLLQ 125

Query: 124 GDPLHCAKFRMDQSMFLLLRKI 146
           GDP H + F +DQ +FLL+RK+
Sbjct: 126 GDPAHISHFELDQRLFLLMRKL 146

[Close]

BLAST of Tan0000631 vs. ExPASy Swiss-Prot
Match: Q54YW8 (DNA-directed RNA polymerases I, II, and III subunit rpabc3 OS=Dictyostelium discoideum OX=44689 GN=polr2h PE=3 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 6.6e-30
Identity = 65/144 (45.14%), Postives = 97/144 (67.36%), Query Frame = 0


Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M  +LF+D F+VK ++PDGKK+D+VSR   +SE   M + +D+ T IYP+   +R  MVL
Sbjct: 1   MTSILFEDTFEVKEIDPDGKKFDRVSRFVCYSENYEMDLQIDIATHIYPLGH-ERFKMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           + +LN DG+   S S    K SLADK++Y+M+GK++K   E S S  KV V+ASFGGL +
Sbjct: 61  ASSLNLDGSSDNSGSYDTNKPSLADKYDYVMYGKIFKYQKENSSS--KVSVFASFGGLLV 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRK 145
           +L+GDP +     +D  ++LL++K
Sbjct: 121 LLQGDPRYLPGIELDARIYLLIKK 141

[Close]

BLAST of Tan0000631 vs. ExPASy Swiss-Prot
Match: Q19826 (Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhabditis elegans OX=6239 GN=rpb-8 PE=1 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 1.9e-29
Identity = 59/145 (40.69%), Postives = 99/145 (68.28%), Query Frame = 0


Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M  ++FDD+FKVK V+PDGKK+D+VSR    +E   M +++D+N++IYP+ + D++ +VL
Sbjct: 1   MAGIIFDDMFKVKSVDPDGKKFDRVSRYFCDAESFKMELIIDINSQIYPLKQNDKVRLVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSD-LKVEVYASFGGLQ 120
           + TL  DG         + +     ++EY+M+GK+Y+L D+ +G+D  K+  YASFGGL 
Sbjct: 61  ATTLREDGLADEGEYDPKAEYPRIKQYEYVMYGKVYRLEDDDTGTDGGKLAAYASFGGLL 120

Query: 121 MMLKGDPLHCAKFRMDQSMFLLLRK 145
           M LKG+ ++   F +D +++LL++K
Sbjct: 121 MRLKGEAINLHGFEVDMNLYLLMKK 145

[Close]

BLAST of Tan0000631 vs. ExPASy Swiss-Prot
Match: A8XT81 (Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhabditis briggsae OX=6238 GN=rpb-8 PE=3 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 2.1e-28
Identity = 58/145 (40.00%), Postives = 97/145 (66.90%), Query Frame = 0


Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M  ++FDD+F VK V+PDGKK+D+VSR    +E   M +++D+N++IYP+ + D+  +VL
Sbjct: 1   MAGIIFDDMFSVKSVDPDGKKFDRVSRYFCDAESFKMELIIDINSQIYPLKQNDKFRLVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSD-LKVEVYASFGGLQ 120
           + TL  DG         + +     ++EY+M+GK+Y+L D+ SG+D  ++  YASFGGL 
Sbjct: 61  ATTLREDGLADEGEYDPKAEYPRIKQYEYVMYGKVYRLEDDDSGTDGGQLAAYASFGGLL 120

Query: 121 MMLKGDPLHCAKFRMDQSMFLLLRK 145
           M LKG+ ++   F +D +++LL++K
Sbjct: 121 MRLKGEAINLHGFEVDMNLYLLMKK 145

[Close]

BLAST of Tan0000631 vs. NCBI nr
Match: XP_022983196.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983203.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983212.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983221.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983229.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima])

HSP 1 Score: 285.4 bits (729), Expect = 2.7e-73
Identity = 138/146 (94.52%), Postives = 144/146 (98.63%), Query Frame = 0


Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M E+LFDDIFKVKCVNPDGKKYDKVSRIEA SEKLSMYMLLDVNTEIYPIDEGD+ILMVL
Sbjct: 1   MAEVLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKL+DEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRKIT 147
           MLKGDPLHCAKFR+DQ+MFLLLRK+T
Sbjct: 121 MLKGDPLHCAKFRVDQNMFLLLRKLT 146

[Close]

BLAST of Tan0000631 vs. NCBI nr
Match: KAG6600626.1 (DNA-directed RNA polymerases II, IV and V subunit 8B, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031262.1 DNA-directed RNA polymerases II, IV and V subunit 8B, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 283.5 bits (724), Expect = 1.0e-72
Identity = 137/146 (93.84%), Postives = 143/146 (97.95%), Query Frame = 0


Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M ELLFDDIFKVKCVNPDGKKYDKVSRIEA SEKLSMYMLLDVNTEIYPIDEGD+ILMVL
Sbjct: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVT YSGQ+GKKSLADKFEYIMHGKLYKL+DEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRKIT 147
           MLKGDPLHCAKFR+DQ+MFLLLRK+T
Sbjct: 121 MLKGDPLHCAKFRVDQNMFLLLRKLT 146

[Close]

BLAST of Tan0000631 vs. NCBI nr
Match: XP_022942165.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata])

HSP 1 Score: 279.6 bits (714), Expect = 1.5e-71
Identity = 136/145 (93.79%), Postives = 141/145 (97.24%), Query Frame = 0


Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M ELLFDDIFKVKCVNPDGKKYDKVSRIEA SEKLSMYMLLDVNTEIYPIDEGD+ILMVL
Sbjct: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMH KLYKL+DEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHRKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRKI 146
           MLKGDPLHC KFR+DQ+MFLLLRK+
Sbjct: 121 MLKGDPLHCVKFRVDQNMFLLLRKL 145

[Close]

BLAST of Tan0000631 vs. NCBI nr
Match: XP_022929200.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata] >XP_022929202.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata] >XP_022985172.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_023551974.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita pepo subsp. pepo] >KAG6577274.1 DNA-directed RNA polymerases II, IV and V subunit 8B, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015358.1 DNA-directed RNA polymerases II, IV and V subunit 8B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 278.9 bits (712), Expect = 2.6e-71
Identity = 133/145 (91.72%), Postives = 143/145 (98.62%), Query Frame = 0


Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M ELLF+D+FKVKCVNPDGKKYDKVSRIEA SEKLSMYMLLDVNTEIYPIDEG++ILMVL
Sbjct: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGK+YKLSDEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRKI 146
           +LKGDP+HCAKFR+DQ+MFLLLRK+
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 145

[Close]

BLAST of Tan0000631 vs. NCBI nr
Match: XP_038901286.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like [Benincasa hispida])

HSP 1 Score: 275.8 bits (704), Expect = 2.2e-70
Identity = 132/144 (91.67%), Postives = 141/144 (97.92%), Query Frame = 0


Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M ELLFDDIF VKCVNPDGKKYDKVSRIEA SEKLSMYMLLDVNTEIYPIDEG++ILMVL
Sbjct: 1   MAELLFDDIFMVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSY+GQEGKKSLADKFEYIMHGK+YKLSD+GSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYTGQEGKKSLADKFEYIMHGKIYKLSDDGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRK 145
           +LKGDP+HCAKFR+DQ+MFLLLRK
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRK 144

[Close]

BLAST of Tan0000631 vs. ExPASy TrEMBL
Match: A0A6J1J6Q9 (DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita maxima OX=3661 GN=LOC111481850 PE=3 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 1.3e-73
Identity = 138/146 (94.52%), Postives = 144/146 (98.63%), Query Frame = 0


Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M E+LFDDIFKVKCVNPDGKKYDKVSRIEA SEKLSMYMLLDVNTEIYPIDEGD+ILMVL
Sbjct: 1   MAEVLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKL+DEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRKIT 147
           MLKGDPLHCAKFR+DQ+MFLLLRK+T
Sbjct: 121 MLKGDPLHCAKFRVDQNMFLLLRKLT 146

[Close]

BLAST of Tan0000631 vs. ExPASy TrEMBL
Match: A0A6J1FVR2 (DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita moschata OX=3662 GN=LOC111447307 PE=3 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 7.3e-72
Identity = 136/145 (93.79%), Postives = 141/145 (97.24%), Query Frame = 0


Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M ELLFDDIFKVKCVNPDGKKYDKVSRIEA SEKLSMYMLLDVNTEIYPIDEGD+ILMVL
Sbjct: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMH KLYKL+DEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHRKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRKI 146
           MLKGDPLHC KFR+DQ+MFLLLRK+
Sbjct: 121 MLKGDPLHCVKFRVDQNMFLLLRKL 145

[Close]

BLAST of Tan0000631 vs. ExPASy TrEMBL
Match: A0A6J1ETP8 (DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita moschata OX=3662 GN=LOC111435865 PE=3 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.2e-71
Identity = 133/145 (91.72%), Postives = 143/145 (98.62%), Query Frame = 0


Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M ELLF+D+FKVKCVNPDGKKYDKVSRIEA SEKLSMYMLLDVNTEIYPIDEG++ILMVL
Sbjct: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGK+YKLSDEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRKI 146
           +LKGDP+HCAKFR+DQ+MFLLLRK+
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 145

[Close]

BLAST of Tan0000631 vs. ExPASy TrEMBL
Match: A0A6J1J454 (DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita maxima OX=3661 GN=LOC111483257 PE=3 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.2e-71
Identity = 133/145 (91.72%), Postives = 143/145 (98.62%), Query Frame = 0


Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           M ELLF+D+FKVKCVNPDGKKYDKVSRIEA SEKLSMYMLLDVNTEIYPIDEG++ILMVL
Sbjct: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGK+YKLSDEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRKI 146
           +LKGDP+HCAKFR+DQ+MFLLLRK+
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 145

[Close]

BLAST of Tan0000631 vs. ExPASy TrEMBL
Match: A0A6J1C3H4 (DNA-directed RNA polymerases II and V subunit 8A-like OS=Momordica charantia OX=3673 GN=LOC111008140 PE=3 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 4.4e-69
Identity = 129/145 (88.97%), Postives = 142/145 (97.93%), Query Frame = 0


Query: 1   MVELLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVL 60
           MVELLFDDIFKVKCVNPDG+KYDKVSRI+A SEKL+M+MLLDVNTEIYPIDEG++ILMVL
Sbjct: 1   MVELLFDDIFKVKCVNPDGQKYDKVSRIDARSEKLAMHMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPV S+S QEGKKSLADKFEY+MHGKLYKLS+EGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVASHSSQEGKKSLADKFEYVMHGKLYKLSEEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRMDQSMFLLLRKI 146
           MLKGDP+HCAKFR+DQ+MFLLLRK+
Sbjct: 121 MLKGDPVHCAKFRVDQNMFLLLRKL 145

[Close]

BLAST of Tan0000631 vs. TAIR 10
Match: AT1G54250.1 (RNA polymerase Rpb8 )

HSP 1 Score: 174.1 bits (440), Expect = 8.3e-44
Identity = 82/142 (57.75%), Postives = 106/142 (74.65%), Query Frame = 0


Query: 4   LLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVLSPT 63
           +LF+DIF V  ++PDGKK+DKV+R++A S  L M+M LDVNTE+YP+  GD+  + L+PT
Sbjct: 6   ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPT 65

Query: 64  LNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQMMLK 123
           LN DG P T Y     KK+LADK+EYIMHGKLYK+S E  G   K E+Y SFGGL M+LK
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKIS-ERDGKTPKAELYVSFGGLLMLLK 125

Query: 124 GDPLHCAKFRMDQSMFLLLRKI 146
           GDP H + F +DQ +FLL+RK+
Sbjct: 126 GDPAHISHFELDQRLFLLMRKL 146

[Close]

BLAST of Tan0000631 vs. TAIR 10
Match: AT3G59600.1 (RNA polymerase Rpb8 )

HSP 1 Score: 171.8 bits (434), Expect = 4.1e-43
Identity = 80/142 (56.34%), Postives = 106/142 (74.65%), Query Frame = 0


Query: 4   LLFDDIFKVKCVNPDGKKYDKVSRIEAWSEKLSMYMLLDVNTEIYPIDEGDRILMVLSPT 63
           ++F+DIF V  ++PDGKK+DKV+R+EA S  L M+M LDVNTE+YP+  GD+  + ++PT
Sbjct: 6   IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMAPT 65

Query: 64  LNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLSDEGSGSDLKVEVYASFGGLQMMLK 123
           LN DG P T Y     KK+LADK+EYIMHGKLYK+S E  G   K E+Y SFGGL M+L+
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKIS-ERDGKTPKAELYVSFGGLLMLLQ 125

Query: 124 GDPLHCAKFRMDQSMFLLLRKI 146
           GDP H + F +DQ +FLL+RK+
Sbjct: 126 GDPAHISHFELDQRLFLLMRKL 146

The following BLAST results are available for this feature:

BLAST of Tan0000631 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81097	1.2e-42	57.75	DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana OX=3702...	[more]
Q9M1A8	5.8e-42	56.34	DNA-directed RNA polymerases II, IV and V subunit 8B OS=Arabidopsis thaliana OX=...	[more]
Q54YW8	6.6e-30	45.14	DNA-directed RNA polymerases I, II, and III subunit rpabc3 OS=Dictyostelium disc...	[more]
Q19826	1.9e-29	40.69	Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhab...	[more]
A8XT81	2.1e-28	40.00	Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhab...	[more]

BLAST of Tan0000631 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022983196.1	2.7e-73	94.52	DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP...	[more]
KAG6600626.1	1.0e-72	93.84	DNA-directed RNA polymerases II, IV and V subunit 8B, partial [Cucurbita argyros...	[more]
XP_022942165.1	1.5e-71	93.79	DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata]	[more]
XP_022929200.1	2.6e-71	91.72	DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata] >...	[more]
XP_038901286.1	2.2e-70	91.67	DNA-directed RNA polymerases II, IV and V subunit 8B-like [Benincasa hispida]	[more]

BLAST of Tan0000631 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J6Q9	1.3e-73	94.52	DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita maxima OX...	[more]
A0A6J1FVR2	7.3e-72	93.79	DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita moschata ...	[more]
A0A6J1ETP8	1.2e-71	91.72	DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita moschata ...	[more]
A0A6J1J454	1.2e-71	91.72	DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita maxima OX...	[more]
A0A6J1C3H4	4.4e-69	88.97	DNA-directed RNA polymerases II and V subunit 8A-like OS=Momordica charantia OX=...	[more]

BLAST of Tan0000631 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G54250.1	8.3e-44	57.75	RNA polymerase Rpb8	[more]
AT3G59600.1	4.1e-43	56.34	RNA polymerase Rpb8	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005570	RNA polymerase, Rpb8	SMART	SM00658	rpol8neu	coord: 2..144 e-value: 6.1E-56 score: 201.8
IPR005570	RNA polymerase, Rpb8	PFAM	PF03870	RNA_pol_Rpb8	coord: 7..144 e-value: 6.7E-49 score: 165.6
IPR005570	RNA polymerase, Rpb8	PIRSF	PIRSF000779	RPB8	coord: 1..146 e-value: 1.2E-46 score: 156.4
IPR005570	RNA polymerase, Rpb8	PANTHER	PTHR10917	DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3	coord: 1..145
None	No IPR available	GENE3D	2.40.50.140		coord: 6..146 e-value: 5.3E-53 score: 180.9
None	No IPR available	PANTHER	PTHR10917:SF1	DNA-DIRECTED RNA POLYMERASE I, II	coord: 1..145
IPR012340	Nucleic acid-binding, OB-fold	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 4..144

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0000631.1	Tan0000631.1	mRNA
Tan0000631.2	Tan0000631.2	mRNA
Tan0000631.3	Tan0000631.3	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0005736	RNA polymerase I complex
cellular_component	GO:0005665	RNA polymerase II, core complex
cellular_component	GO:0005666	RNA polymerase III complex
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity