Tan0000012.3 (mRNA) Snake gourd v1

BLAST of Tan0000012.3 vs. ExPASy Swiss-Prot
Match: O23530 (Protein SUPPRESSOR OF npr1-1, CONSTITUTIVE 1 OS=Arabidopsis thaliana OX=3702 GN=SNC1 PE=1 SV=5)

HSP 1 Score: 202.2 bits (513), Expect = 1.9e-50
Identity = 166/513 (32.36%), Postives = 256/513 (49.90%), Query Frame = 0

Query: 2    LRSLEFLNLRGCEKLQEIPDLSIASNLKKLDLNGCINLRIIHESDFSLDKLVILNLGNCA 61
            L SL+ +NLR    L+EIPDLS+A NL++LDL GC +L  +  S  +  KL+ L++ +C 
Sbjct: 621  LGSLKEMNLRYSNNLKEIPDLSLAINLEELDLVGCKSLVTLPSSIQNATKLIYLDMSDCK 680

Query: 62   NLEKVPSYLMSKSLEFLNLRKCKKLKEI---------YDFSAASNIQRVYLGECISLRII 121
             LE  P+ L  +SLE+LNL  C  L+            DF    N   + + +C      
Sbjct: 681  KLESFPTDLNLESLEYLNLTGCPNLRNFPAIKMGCSDVDFPEGRN--EIVVEDC----FW 740

Query: 122  HEFVGRSLDKLVALEIKYCYNLKRLPCCFMSKSLQVLDLTLCRKIIKFPDFSFEKLPSPL 181
            ++ +   LD L  L        + +PC F  + L  L++           +  EKL   +
Sbjct: 741  NKNLPAGLDYLDCL-------TRCMPCEFRPEQLAFLNVR---------GYKHEKLWEGI 800

Query: 182  K-LKSLRVVSLSMCDNLEEITELSIASNLEVLDLSHCFNLRIIHECIGSLDKLVTLNLTD 241
            + L SL  + LS  +NL EI +LS A+ LE L L++C +L  +   IG+L +LV L + +
Sbjct: 801  QSLGSLEGMDLSESENLTEIPDLSKATKLESLILNNCKSLVTLPSTIGNLHRLVRLEMKE 860

Query: 242  CSNLEKLPNFSAALNLQELYLGNCTSLRT--------------------IHESIGSLNKL 301
            C+ LE LP      +L+ L L  C+SLR+                    I  +IG+L++L
Sbjct: 861  CTGLEVLPTDVNLSSLETLDLSGCSSLRSFPLISTNIVWLYLENTAIEEIPSTIGNLHRL 920

Query: 302  VTLDLRGCSNVEKLPSYLRLKFLDCLNLSDYRRLEQLPEFDENMKSLRRMDLEFSAIKKL 361
            V L+++ C+ +E LP+ + L  L+ L+LS    L   P   E++K L    LE +AI+++
Sbjct: 921  VRLEMKKCTGLEVLPTDVNLSSLETLDLSGCSSLRSFPLISESIKWLY---LENTAIEEI 980

Query: 362  PLSIGNLTKLKFLSLRGCANLIALPSTIYLLESLERLCLRGCSKLFMFPQPSSLKVPQES 421
            P  +   T LK L L  C +L+ LP+TI  L+ L    ++ C+ L + P   +L      
Sbjct: 981  P-DLSKATNLKNLKLNNCKSLVTLPTTIGNLQKLVSFEMKECTGLEVLPIDVNL------ 1040

Query: 422  LCSKLTFLDLKNCNILNVDFLETLSRVSTSLFELHLSGNKFSRLPSCLHNFTSLRELHLK 481
              S L  LDL  C+      L T   +ST++  L+L       +PS + N   L +L +K
Sbjct: 1041 --SSLMILDLSGCS-----SLRTFPLISTNIVWLYLENTAIEEIPSTIGNLHRLVKLEMK 1093

BLAST of Tan0000012.3 vs. ExPASy Swiss-Prot
Match: F4JNB7 (Disease resistance protein RPP5 OS=Arabidopsis thaliana OX=3702 GN=RPP5 PE=1 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 2.9e-43
Identity = 164/562 (29.18%), Postives = 258/562 (45.91%), Query Frame = 0

Query: 2    LRSLEFLNLRGCEKLQEIPDLSIASNLKKLDLNGC-------------INLRIIH----- 61
            L SL+ +NL   + L+EIPDLS A NL++LDL GC             I LR +H     
Sbjct: 618  LGSLKKMNLLCSKNLKEIPDLSNARNLEELDLEGCESLVTLPSSIQNAIKLRKLHCSGVI 677

Query: 62   -------------------------------------------------ESDFSLDKLVI 121
                                                              S+F ++ LV 
Sbjct: 678  LIDLKSLEGMCNLEYLSVDCSRVEGTQGIVYFPSKLRLLLWNNCPLKRLHSNFKVEYLVK 737

Query: 122  LNLGNCANLEKVPSYLMSKS-LEFLNLRKCKKLKEIYDFSAASNIQRVYLGECISLRIIH 181
            L + N ++LEK+         L+ + LR  K LKEI D S A N++ V + +C SL    
Sbjct: 738  LRMEN-SDLEKLWDGTQPLGRLKQMFLRGSKYLKEIPDLSLAINLEEVDICKCESLVTFP 797

Query: 182  EFVGRSLDKLVALEIKYCYNLKRLPCCFMSKSLQVLDLTLCRKIIKFP-------DFSFE 241
              +  ++ KL+ L+I  C  L+  P     +SL+ L+LT C  +  FP       D  F 
Sbjct: 798  SSMQNAI-KLIYLDISDCKKLESFPTDLNLESLEYLNLTGCPNLRNFPAIKMGCSDVDFP 857

Query: 242  KLPSPLKLKSL-----RVVSLSMCDNLEEITELSIASNLEVLDLSHCFNLRIIHECIGSL 301
            +  + + ++           L   D L             V     C+    + E I SL
Sbjct: 858  EGRNEIVVEDCFWNKNLPAGLDYLDCLMRCMPCEFRPEYLVFLNVRCYKHEKLWEGIQSL 917

Query: 302  DKLVTLNLTDCSNLEKLPNFSAALNLQELYLGNCTSLRTIHESIGSLNKLVTLDLRGCSN 361
              L  ++L++  NL ++P+ S A NL+ LYL NC SL T+  +IG+L KLV L+++ C+ 
Sbjct: 918  GSLEEMDLSESENLTEIPDLSKATNLKHLYLNNCKSLVTLPSTIGNLQKLVRLEMKECTG 977

Query: 362  VEKLPSYLRLKFLDCLNLSDYRRLEQLPEFDENMKSLRRMDLEFSAIKKLPLSIGNLTKL 421
            +E LP+ + L  L+ L+LS    L   P      KS++ + LE +AI+++ L +   TKL
Sbjct: 978  LEVLPTDVNLSSLETLDLSGCSSLRTFPLIS---KSIKWLYLENTAIEEI-LDLSKATKL 1037

Query: 422  KFLSLRGCANLIALPSTIYLLESLERLCLRGCSKLFMFPQPSSLKVPQESLCSKLTFLDL 481
            + L L  C +L+ LPSTI  L++L RL ++ C+ L + P   +L        S L  LDL
Sbjct: 1038 ESLILNNCKSLVTLPSTIGNLQNLRRLYMKRCTGLEVLPTDVNL--------SSLGILDL 1097

BLAST of Tan0000012.3 vs. ExPASy Swiss-Prot
Match: F4JT82 (Probable disease resistance protein At4g19520 OS=Arabidopsis thaliana OX=3702 GN=At4g19520 PE=3 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 4.9e-35
Identity = 141/498 (28.31%), Postives = 240/498 (48.19%), Query Frame = 0

Query: 2    LRSLEFLNLRGCEKLQEIPDLSIASNLKKLDLNGCINLRIIHESDFSLDKLVILNLGNCA 61
            L  L+ + L    +L  + +L  + N++K+DL GC+ L+   ++   L  L I++L  C 
Sbjct: 602  LEVLKRITLSCSVQLLNVDELQYSPNIEKIDLKGCLELQSFPDTG-QLQHLRIVDLSTCK 661

Query: 62   NLEKVPSYLMSKSLEFLNLRKCKKLKEIYDFSAASNIQRVYLGECISLRIIHEFVGRSLD 121
             ++  P   +  S+  L+L+    ++++   + +S  QR      ++ ++ +        
Sbjct: 662  KIKSFPK--VPPSIRKLHLQG-TGIRDLSSLNHSSESQR------LTRKLENVSSSNQDH 721

Query: 122  KLVALEIKYCYNLKRLPCCFMSKSLQVLDLTLCRKIIKFPDFSFEKLPSPLKLKSLRVVS 181
            +   L++K   +L  LP   + +SL+VLD + C ++     F        L   +++ V 
Sbjct: 722  RKQVLKLKDSSHLGSLPDIVIFESLEVLDFSGCSELEDIQGFPQNLKRLYLAKTAIKEVP 781

Query: 182  LSMCDNLEEITELSIASNLEVLDLSHCFNLRIIHECIGSLDKLVTLNLTDCSNLEKLPNF 241
             S+C ++         S L  LD+ +C  LR +   + ++  L  L L+ CSNLE +   
Sbjct: 782  SSLCHHI---------SKLVKLDMENCERLRDLPMGMSNMKYLAVLKLSGCSNLENIKEL 841

Query: 242  SAALNLQELYLGNCTSLRTIHESIGSLNKLVTLDLRGCSNVEKLPSYL-RLKFLDCLNLS 301
                NL+ELYL            + +L+++V LDL  C  ++ LP+ + +L+FL  L LS
Sbjct: 842  PR--NLKELYLAGTAVKEFPSTLLETLSEVVLLDLENCKKLQGLPTGMSKLEFLVMLKLS 901

Query: 302  DYRRLEQLPEFDENMKSLRRMDLEFSAIKKLPLSIGNLTKLKFLSLRGCANLIALPSTIY 361
               +LE + +   N+  L    L  +AI++LP SIG+L  L  L L+ C  L  LP  ++
Sbjct: 902  GCSKLEIIVDLPLNLIELY---LAGTAIRELPPSIGDLALLDTLDLKNCNRLRHLPMEMH 961

Query: 362  LLESLERLCLRGCSKLFMFPQPSSLKVPQE--------SLCSKLTFLDLKNCNILNVDFL 421
             L  L+ L L  CS+L +F   SSL   +E         L SKL F     C  +  +  
Sbjct: 962  NLNPLKVLDLSNCSELEVF--TSSLPKVRELRPAPTVMLLRSKLPF-----CFFIFYEHR 1021

Query: 422  ETLS-------------RVSTSLFELHLSGNKFSRLPSCLHNFTSLRELHLKNCKFLQEI 478
             TLS             R   SL  L LS N F+ +P  + +F+ L  L L+ C+ L+ +
Sbjct: 1022 VTLSLYKARLQYIPEEIRWMPSLKTLDLSRNGFTEVPVSIKDFSKLLSLRLRYCENLRSL 1068

BLAST of Tan0000012.3 vs. ExPASy Swiss-Prot
Match: V9M2S5 (Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 1.9e-31
Identity = 118/365 (32.33%), Postives = 183/365 (50.14%), Query Frame = 0

Query: 174  LKSLRVVSLSMCDNLEEITELSIASNLEVLDLSHCFNLRIIHECIGSLDKLVTLNLTDCS 233
            L+ L+V+ LS    L +++E S   NLE L LS C +L  IH  +G++ KL TL+L  C+
Sbjct: 655  LERLKVIDLSYSRKLSQMSEFSSMPNLERLCLSGCVSLIDIHPSVGNMKKLTTLSLRSCN 714

Query: 234  NLEKLPNFSAAL-NLQELYLGNCTSLRTIHESIGSLNKLVTLDLRGCSNVEKLPSYLRLK 293
             L+ LP+    L +L+ LYL NC+      E  G++  L  LDL+  +  +   S   L+
Sbjct: 715  KLKNLPDSIGDLESLESLYLSNCSKFEKFPEKGGNMKSLTELDLKNTAIKDLPDSIGDLE 774

Query: 294  FLDCLNLSDYRRLEQLPEFDENMKSLRRMDLEFSAIKKLPLSIGNLTKLKFLSLRGCAN- 353
             L+ L LS+  + E+ PE   NMKSL  +DL+ +AIK LP SIG+L  L+ L+L  CA  
Sbjct: 775  SLESLYLSNCSKFEKFPEKGGNMKSLTELDLKNTAIKDLPDSIGDLESLEILNLSDCAKF 834

Query: 354  ----------------------LIALPSTIYLLESLERLCLRGCSKLFMFPQP-SSLKVP 413
                                  +  LP +I  L+SL+ L L  CSK   FP+   ++K  
Sbjct: 835  EKFPEKGGNMKSLKELDLQNTAIKDLPDSIGDLKSLKYLSLSDCSKFEKFPEKGGNMKRL 894

Query: 414  QESLCSKLTFLDLKNCNILNVDFLETLSRVSTSLFELHLSG-NKFSRLPSCLHNFTSLRE 473
             + + S     DL +    ++  LE       SL  L+LS  +KF + P    N  SL E
Sbjct: 895  LQLILSNTAIKDLPD----SIGDLE-------SLKYLYLSDCSKFEKFPEKGGNMKSLTE 954

Query: 474  LHLKNCKFLQEIPNL---PQSIQIMDASGCESLVRSPDNIADIIDYEFDSSKREFILMNS 510
            L LKN   ++++P+     +S++I++ S C    + P+   ++       S +E  L N+
Sbjct: 955  LDLKNTA-IKDLPDSIGDLESLEILNLSDCAKFEKFPEKGGNM------KSLKELDLQNT 1001

BLAST of Tan0000012.3 vs. ExPASy Swiss-Prot
Match: A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 3.6e-30
Identity = 120/453 (26.49%), Postives = 201/453 (44.37%), Query Frame = 0

Query: 45   SDFSLDKLVILNLGNCANLEKVPSYLMSKSLEFLNLRKCKKLKEIYDFSAASNIQRVYLG 104
            S+F   KLV L +   + +E          L  L+L  C KL +  DF   +N++R+ L 
Sbjct: 595  SNFEPSKLVYLTMKGSSIIELWNGAKRLAFLTTLDLSYCHKLIQTPDFRMITNLERLILS 654

Query: 105  ECISLRIIHEFVGRSLDKLVALEIKYCYNLKRLPCCFMSKSLQVLDLTLCRKIIKFPDFS 164
             C +L  +H  VG  L  L+ L + +C +L+RLP    S+ L+VLDL  C  +  FP+  
Sbjct: 655  SCDALVEVHPSVG-FLKNLILLNMDHCISLERLPAIIQSECLEVLDLNYCFNLKMFPEVE 714

Query: 165  FEKLPSPLKLKSLRVVSLSMCDNLEEITELSIASNLEVLDLSHCFNLRIIHECIGSLDKL 224
                 +   LK L + S  + +    I  LS   NL++   +   +L        S+ + 
Sbjct: 715  ----RNMTHLKKLDLTSTGIRELPASIEHLSSLENLQMHSCNQLVSLP------SSIWRF 774

Query: 225  VTLNLTDCSNLEKLPNFSAALNLQELYLGNCTSLRTIHESIGSLNKLVTLDLRGCSNVEK 284
              L +++C  L  LP      N     +    S++ +  SIG+L  L  L++  C  +  
Sbjct: 775  RNLKISECEKLGSLPEIHGNSNCTRELILKLVSIKELPTSIGNLTSLNFLEICNCKTISS 834

Query: 285  LPSYL-RLKFLDCLNLSDYRRLEQLPEFDENMKSLRRMDLEFSAIKKLPLSIGNLTKLKF 344
            L S +  L  L  L L D R+L+ LP     +  L    L+     + P     L  L+ 
Sbjct: 835  LSSSIWGLTSLTTLKLLDCRKLKNLPGIPNAINHLSGHGLQLLLTLEQPTIYERLDLLRI 894

Query: 345  LSLRGCANLIALPSTIYLLESLERLCLRGCSKLFMFPQ---------------PSSLKVP 404
            + +  C+ + +LP  I++L+ L  LC+  CS+L   P+                  L++P
Sbjct: 895  IDMSWCSCISSLPHNIWMLKFLRILCISYCSRLEYLPENLGHLEHLEELLADGTGILRLP 954

Query: 405  QE-SLCSKLTFLDLK-----------NCNILNV--DFLETLSRVSTSLFELHLSGNKFSR 464
               +  +KL  L  +           + ++LN+  D   +L  +  S+ +L+LSGN F  
Sbjct: 955  SSVARLNKLEVLSFRKKFAIGPKVQYSSSMLNLPDDVFGSLGSLG-SVVKLNLSGNGFCN 1014

Query: 465  LPSCLHNFTSLRELHLKNCKFLQEIPNLPQSIQ 468
            LP  ++    L  L +  C+ L+ +P LP SI+
Sbjct: 1015 LPETMNQLFCLEYLDITFCQRLEALPELPPSIK 1035

BLAST of Tan0000012.3 vs. NCBI nr
Match: XP_023537666.1 (disease resistance protein RPS6-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023537667.1 disease resistance protein RPS6-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_023537668.1 disease resistance protein RPS6-like isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 505.4 bits (1300), Expect = 7.8e-139
Identity = 305/629 (48.49%), Postives = 382/629 (60.73%), Query Frame = 0

Query: 15   KLQEIPDLSIASNLKKLDLNGCINLRIIHESDFSLDKLVILNLGNCANLEKVPSYLMSKS 74
            +L++IPD S +S+L+KL LN CINLR I  S  SL KLVIL+L +C+NL+K+PSYL  KS
Sbjct: 641  RLEKIPDFSTSSSLEKLYLNHCINLRAIPMSVVSLSKLVILDLNHCSNLKKLPSYLRLKS 700

Query: 75   LEFLNLRKCKKLKEIYDFSAASNIQRVYLGECISLRIIHEFVGRSLDKLVALEIKYCYNL 134
            L+ LNL  CKKL+E+ +FSA SN++++YL EC +LR+IHE +G SLD+LV L+++ C NL
Sbjct: 701  LKVLNLNYCKKLEELPNFSAVSNLEKLYLKECTNLRMIHESIG-SLDELVTLDLEKCSNL 760

Query: 135  KRLPCCFMSKSLQVLDLTLCRKIIKFPDFSFEKLPSPLKLKSLRVVSLSMCDNLEEITEL 194
            K+LP C                               L LKSL+                
Sbjct: 761  KKLPGC-------------------------------LSLKSLK---------------- 820

Query: 195  SIASNLEVLDLSHCFNLRIIHECIGSLDKLVTLNLTDCSNLEKLPNFSAALNLQELYLGN 254
                                            LNL  C  L+++P+FSAALNLQ LYL  
Sbjct: 821  -------------------------------HLNLDHCKKLQEIPDFSAALNLQSLYLKK 880

Query: 255  CTSLRTIHESIGSLNKLVTLDLRGCSNVEKLPSYLRLKFLDCLNLSDYRRLEQLPEFDEN 314
            CT+LR +HESIGSL+ LV LD+R C+ +EKLPSYL+LK L  L LS   +LE  P+  EN
Sbjct: 881  CTNLRVLHESIGSLDNLVVLDVRHCTKLEKLPSYLKLKSLKYLELSGCSKLEMFPKIAEN 940

Query: 315  MKSLRRMDLEFSAIKKLPLSIGNLTKLKFLSLRGCANLIALPSTIYLLESLERLCLRGCS 374
            MKSL  + L+ + IKKLP SIGNLT+LK L+L GC NLI++P  IYLL++LE L L GCS
Sbjct: 941  MKSLVLLHLDSTGIKKLPSSIGNLTELKVLNLDGCTNLISVPGIIYLLQNLEELHLGGCS 1000

Query: 375  KLFMFPQPSSLKV-----PQESLCSKLTFLDLKNCNILNVDFLETLSRVSTSLFELHLSG 434
            +  M P      +     P   + SKLT LDL++CN+ N DFLE L  V+  L  L LS 
Sbjct: 1001 RFEMLPHKWDSPIHHGNLPTNPMFSKLTLLDLQHCNLSNADFLEILCDVNPFLNSLLLSK 1060

Query: 435  NKFSRLPSCLHNFTSLRELHLKNCKFLQEIPNLPQSIQIMDASGCESLVRSPDNIADII- 494
            NKF  LPSCLH F SL  L L+NCKFLQ+IPNLPQ IQ MDA+GCESLVRSPDNI DII 
Sbjct: 1061 NKFCSLPSCLHKFMSLWNLQLRNCKFLQQIPNLPQCIQRMDATGCESLVRSPDNILDIIS 1120

Query: 495  ---DYEFDSSKREFILMNSEIPELFSHQTRISSTSFNIQHHSDKGRALAACVVFKVNGYS 554
               D       REFILMN EIPE FSHQT  +S   N +H  +  R LA    F+V+G S
Sbjct: 1121 SKQDITLGDFPREFILMNIEIPEWFSHQTISNSIKVNFRHDHNIERTLATSAKFRVDGDS 1180

Query: 555  DKAIAFISYHVLIDGKIWDSFWRRLFPTSTSEYMWLVTT--PLKFSSLGINDRHEIKVWF 614
             +  A IS ++ I  ++  SF R+  P STSEY WLVTT  P   SSL  ND +++ VWF
Sbjct: 1181 YEGEALISCNIFIGYRLQSSFMRKFAP-STSEYTWLVTTSSPTFSSSLATNDWNDVIVWF 1189

Query: 615  EVYKIYNAIVTIRSQGVHV---IDGLQAD 630
            EV K     VTIRS GVH+   +DG+Q D
Sbjct: 1241 EVVKSREVNVTIRSCGVHLTDEVDGIQND 1189

BLAST of Tan0000012.3 vs. NCBI nr
Match: XP_023537672.1 (disease resistance protein RPS6-like isoform X6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 505.4 bits (1300), Expect = 7.8e-139
Identity = 305/629 (48.49%), Postives = 382/629 (60.73%), Query Frame = 0

Query: 15   KLQEIPDLSIASNLKKLDLNGCINLRIIHESDFSLDKLVILNLGNCANLEKVPSYLMSKS 74
            +L++IPD S +S+L+KL LN CINLR I  S  SL KLVIL+L +C+NL+K+PSYL  KS
Sbjct: 532  RLEKIPDFSTSSSLEKLYLNHCINLRAIPMSVVSLSKLVILDLNHCSNLKKLPSYLRLKS 591

Query: 75   LEFLNLRKCKKLKEIYDFSAASNIQRVYLGECISLRIIHEFVGRSLDKLVALEIKYCYNL 134
            L+ LNL  CKKL+E+ +FSA SN++++YL EC +LR+IHE +G SLD+LV L+++ C NL
Sbjct: 592  LKVLNLNYCKKLEELPNFSAVSNLEKLYLKECTNLRMIHESIG-SLDELVTLDLEKCSNL 651

Query: 135  KRLPCCFMSKSLQVLDLTLCRKIIKFPDFSFEKLPSPLKLKSLRVVSLSMCDNLEEITEL 194
            K+LP C                               L LKSL+                
Sbjct: 652  KKLPGC-------------------------------LSLKSLK---------------- 711

Query: 195  SIASNLEVLDLSHCFNLRIIHECIGSLDKLVTLNLTDCSNLEKLPNFSAALNLQELYLGN 254
                                            LNL  C  L+++P+FSAALNLQ LYL  
Sbjct: 712  -------------------------------HLNLDHCKKLQEIPDFSAALNLQSLYLKK 771

Query: 255  CTSLRTIHESIGSLNKLVTLDLRGCSNVEKLPSYLRLKFLDCLNLSDYRRLEQLPEFDEN 314
            CT+LR +HESIGSL+ LV LD+R C+ +EKLPSYL+LK L  L LS   +LE  P+  EN
Sbjct: 772  CTNLRVLHESIGSLDNLVVLDVRHCTKLEKLPSYLKLKSLKYLELSGCSKLEMFPKIAEN 831

Query: 315  MKSLRRMDLEFSAIKKLPLSIGNLTKLKFLSLRGCANLIALPSTIYLLESLERLCLRGCS 374
            MKSL  + L+ + IKKLP SIGNLT+LK L+L GC NLI++P  IYLL++LE L L GCS
Sbjct: 832  MKSLVLLHLDSTGIKKLPSSIGNLTELKVLNLDGCTNLISVPGIIYLLQNLEELHLGGCS 891

Query: 375  KLFMFPQPSSLKV-----PQESLCSKLTFLDLKNCNILNVDFLETLSRVSTSLFELHLSG 434
            +  M P      +     P   + SKLT LDL++CN+ N DFLE L  V+  L  L LS 
Sbjct: 892  RFEMLPHKWDSPIHHGNLPTNPMFSKLTLLDLQHCNLSNADFLEILCDVNPFLNSLLLSK 951

Query: 435  NKFSRLPSCLHNFTSLRELHLKNCKFLQEIPNLPQSIQIMDASGCESLVRSPDNIADII- 494
            NKF  LPSCLH F SL  L L+NCKFLQ+IPNLPQ IQ MDA+GCESLVRSPDNI DII 
Sbjct: 952  NKFCSLPSCLHKFMSLWNLQLRNCKFLQQIPNLPQCIQRMDATGCESLVRSPDNILDIIS 1011

Query: 495  ---DYEFDSSKREFILMNSEIPELFSHQTRISSTSFNIQHHSDKGRALAACVVFKVNGYS 554
               D       REFILMN EIPE FSHQT  +S   N +H  +  R LA    F+V+G S
Sbjct: 1012 SKQDITLGDFPREFILMNIEIPEWFSHQTISNSIKVNFRHDHNIERTLATSAKFRVDGDS 1071

Query: 555  DKAIAFISYHVLIDGKIWDSFWRRLFPTSTSEYMWLVTT--PLKFSSLGINDRHEIKVWF 614
             +  A IS ++ I  ++  SF R+  P STSEY WLVTT  P   SSL  ND +++ VWF
Sbjct: 1072 YEGEALISCNIFIGYRLQSSFMRKFAP-STSEYTWLVTTSSPTFSSSLATNDWNDVIVWF 1080

Query: 615  EVYKIYNAIVTIRSQGVHV---IDGLQAD 630
            EV K     VTIRS GVH+   +DG+Q D
Sbjct: 1132 EVVKSREVNVTIRSCGVHLTDEVDGIQND 1080

BLAST of Tan0000012.3 vs. NCBI nr
Match: XP_023537669.1 (disease resistance protein RPS6-like isoform X4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 505.4 bits (1300), Expect = 7.8e-139
Identity = 305/629 (48.49%), Postives = 382/629 (60.73%), Query Frame = 0

Query: 15   KLQEIPDLSIASNLKKLDLNGCINLRIIHESDFSLDKLVILNLGNCANLEKVPSYLMSKS 74
            +L++IPD S +S+L+KL LN CINLR I  S  SL KLVIL+L +C+NL+K+PSYL  KS
Sbjct: 641  RLEKIPDFSTSSSLEKLYLNHCINLRAIPMSVVSLSKLVILDLNHCSNLKKLPSYLRLKS 700

Query: 75   LEFLNLRKCKKLKEIYDFSAASNIQRVYLGECISLRIIHEFVGRSLDKLVALEIKYCYNL 134
            L+ LNL  CKKL+E+ +FSA SN++++YL EC +LR+IHE +G SLD+LV L+++ C NL
Sbjct: 701  LKVLNLNYCKKLEELPNFSAVSNLEKLYLKECTNLRMIHESIG-SLDELVTLDLEKCSNL 760

Query: 135  KRLPCCFMSKSLQVLDLTLCRKIIKFPDFSFEKLPSPLKLKSLRVVSLSMCDNLEEITEL 194
            K+LP C                               L LKSL+                
Sbjct: 761  KKLPGC-------------------------------LSLKSLK---------------- 820

Query: 195  SIASNLEVLDLSHCFNLRIIHECIGSLDKLVTLNLTDCSNLEKLPNFSAALNLQELYLGN 254
                                            LNL  C  L+++P+FSAALNLQ LYL  
Sbjct: 821  -------------------------------HLNLDHCKKLQEIPDFSAALNLQSLYLKK 880

Query: 255  CTSLRTIHESIGSLNKLVTLDLRGCSNVEKLPSYLRLKFLDCLNLSDYRRLEQLPEFDEN 314
            CT+LR +HESIGSL+ LV LD+R C+ +EKLPSYL+LK L  L LS   +LE  P+  EN
Sbjct: 881  CTNLRVLHESIGSLDNLVVLDVRHCTKLEKLPSYLKLKSLKYLELSGCSKLEMFPKIAEN 940

Query: 315  MKSLRRMDLEFSAIKKLPLSIGNLTKLKFLSLRGCANLIALPSTIYLLESLERLCLRGCS 374
            MKSL  + L+ + IKKLP SIGNLT+LK L+L GC NLI++P  IYLL++LE L L GCS
Sbjct: 941  MKSLVLLHLDSTGIKKLPSSIGNLTELKVLNLDGCTNLISVPGIIYLLQNLEELHLGGCS 1000

Query: 375  KLFMFPQPSSLKV-----PQESLCSKLTFLDLKNCNILNVDFLETLSRVSTSLFELHLSG 434
            +  M P      +     P   + SKLT LDL++CN+ N DFLE L  V+  L  L LS 
Sbjct: 1001 RFEMLPHKWDSPIHHGNLPTNPMFSKLTLLDLQHCNLSNADFLEILCDVNPFLNSLLLSK 1060

Query: 435  NKFSRLPSCLHNFTSLRELHLKNCKFLQEIPNLPQSIQIMDASGCESLVRSPDNIADII- 494
            NKF  LPSCLH F SL  L L+NCKFLQ+IPNLPQ IQ MDA+GCESLVRSPDNI DII 
Sbjct: 1061 NKFCSLPSCLHKFMSLWNLQLRNCKFLQQIPNLPQCIQRMDATGCESLVRSPDNILDIIS 1120

Query: 495  ---DYEFDSSKREFILMNSEIPELFSHQTRISSTSFNIQHHSDKGRALAACVVFKVNGYS 554
               D       REFILMN EIPE FSHQT  +S   N +H  +  R LA    F+V+G S
Sbjct: 1121 SKQDITLGDFPREFILMNIEIPEWFSHQTISNSIKVNFRHDHNIERTLATSAKFRVDGDS 1180

Query: 555  DKAIAFISYHVLIDGKIWDSFWRRLFPTSTSEYMWLVTT--PLKFSSLGINDRHEIKVWF 614
             +  A IS ++ I  ++  SF R+  P STSEY WLVTT  P   SSL  ND +++ VWF
Sbjct: 1181 YEGEALISCNIFIGYRLQSSFMRKFAP-STSEYTWLVTTSSPTFSSSLATNDWNDVIVWF 1189

Query: 615  EVYKIYNAIVTIRSQGVHV---IDGLQAD 630
            EV K     VTIRS GVH+   +DG+Q D
Sbjct: 1241 EVVKSREVNVTIRSCGVHLTDEVDGIQND 1189

BLAST of Tan0000012.3 vs. NCBI nr
Match: XP_023537671.1 (disease resistance protein RPS6-like isoform X5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 505.4 bits (1300), Expect = 7.8e-139
Identity = 305/629 (48.49%), Postives = 382/629 (60.73%), Query Frame = 0

Query: 15   KLQEIPDLSIASNLKKLDLNGCINLRIIHESDFSLDKLVILNLGNCANLEKVPSYLMSKS 74
            +L++IPD S +S+L+KL LN CINLR I  S  SL KLVIL+L +C+NL+K+PSYL  KS
Sbjct: 545  RLEKIPDFSTSSSLEKLYLNHCINLRAIPMSVVSLSKLVILDLNHCSNLKKLPSYLRLKS 604

Query: 75   LEFLNLRKCKKLKEIYDFSAASNIQRVYLGECISLRIIHEFVGRSLDKLVALEIKYCYNL 134
            L+ LNL  CKKL+E+ +FSA SN++++YL EC +LR+IHE +G SLD+LV L+++ C NL
Sbjct: 605  LKVLNLNYCKKLEELPNFSAVSNLEKLYLKECTNLRMIHESIG-SLDELVTLDLEKCSNL 664

Query: 135  KRLPCCFMSKSLQVLDLTLCRKIIKFPDFSFEKLPSPLKLKSLRVVSLSMCDNLEEITEL 194
            K+LP C                               L LKSL+                
Sbjct: 665  KKLPGC-------------------------------LSLKSLK---------------- 724

Query: 195  SIASNLEVLDLSHCFNLRIIHECIGSLDKLVTLNLTDCSNLEKLPNFSAALNLQELYLGN 254
                                            LNL  C  L+++P+FSAALNLQ LYL  
Sbjct: 725  -------------------------------HLNLDHCKKLQEIPDFSAALNLQSLYLKK 784

Query: 255  CTSLRTIHESIGSLNKLVTLDLRGCSNVEKLPSYLRLKFLDCLNLSDYRRLEQLPEFDEN 314
            CT+LR +HESIGSL+ LV LD+R C+ +EKLPSYL+LK L  L LS   +LE  P+  EN
Sbjct: 785  CTNLRVLHESIGSLDNLVVLDVRHCTKLEKLPSYLKLKSLKYLELSGCSKLEMFPKIAEN 844

Query: 315  MKSLRRMDLEFSAIKKLPLSIGNLTKLKFLSLRGCANLIALPSTIYLLESLERLCLRGCS 374
            MKSL  + L+ + IKKLP SIGNLT+LK L+L GC NLI++P  IYLL++LE L L GCS
Sbjct: 845  MKSLVLLHLDSTGIKKLPSSIGNLTELKVLNLDGCTNLISVPGIIYLLQNLEELHLGGCS 904

Query: 375  KLFMFPQPSSLKV-----PQESLCSKLTFLDLKNCNILNVDFLETLSRVSTSLFELHLSG 434
            +  M P      +     P   + SKLT LDL++CN+ N DFLE L  V+  L  L LS 
Sbjct: 905  RFEMLPHKWDSPIHHGNLPTNPMFSKLTLLDLQHCNLSNADFLEILCDVNPFLNSLLLSK 964

Query: 435  NKFSRLPSCLHNFTSLRELHLKNCKFLQEIPNLPQSIQIMDASGCESLVRSPDNIADII- 494
            NKF  LPSCLH F SL  L L+NCKFLQ+IPNLPQ IQ MDA+GCESLVRSPDNI DII 
Sbjct: 965  NKFCSLPSCLHKFMSLWNLQLRNCKFLQQIPNLPQCIQRMDATGCESLVRSPDNILDIIS 1024

Query: 495  ---DYEFDSSKREFILMNSEIPELFSHQTRISSTSFNIQHHSDKGRALAACVVFKVNGYS 554
               D       REFILMN EIPE FSHQT  +S   N +H  +  R LA    F+V+G S
Sbjct: 1025 SKQDITLGDFPREFILMNIEIPEWFSHQTISNSIKVNFRHDHNIERTLATSAKFRVDGDS 1084

Query: 555  DKAIAFISYHVLIDGKIWDSFWRRLFPTSTSEYMWLVTT--PLKFSSLGINDRHEIKVWF 614
             +  A IS ++ I  ++  SF R+  P STSEY WLVTT  P   SSL  ND +++ VWF
Sbjct: 1085 YEGEALISCNIFIGYRLQSSFMRKFAP-STSEYTWLVTTSSPTFSSSLATNDWNDVIVWF 1093

Query: 615  EVYKIYNAIVTIRSQGVHV---IDGLQAD 630
            EV K     VTIRS GVH+   +DG+Q D
Sbjct: 1145 EVVKSREVNVTIRSCGVHLTDEVDGIQND 1093

BLAST of Tan0000012.3 vs. NCBI nr
Match: KGN65609.2 (hypothetical protein Csa_019785 [Cucumis sativus])

HSP 1 Score: 497.3 bits (1279), Expect = 2.1e-136
Identity = 320/641 (49.92%), Postives = 408/641 (63.65%), Query Frame = 0

Query: 3    RSLEFLNLRGCEKLQEIPDLSIASNLKKLDLNGCINLRIIHESDFSLDKLVILNLGNCAN 62
            + L+ ++L     L+E PD S A NL+KL L  C  L++IH S  SL KLV L+L  C N
Sbjct: 602  KMLKHVDLSYWRLLEETPDFSAALNLEKLYLLSCKRLKMIHGSVASLSKLVTLDLEGCEN 661

Query: 63   LEKVP-SYLMSKSLEFLNLRKCKKLKEIYDFSAASNIQRVYLGECISLRIIHE-FVGRSL 122
            LEK+P S+LM KSLE LNL  C KLKEI D SA+SN++ ++L EC  LRIIH+  VGR L
Sbjct: 662  LEKLPSSFLMLKSLEVLNLSGCIKLKEIPDLSASSNLKELHLRECYHLRIIHDSAVGRFL 721

Query: 123  DKLVALEIKYCYNLKRLPCCFM-SKSLQVLDLTLCRKIIKFPDFSFEKLPSPLKLKSLRV 182
            DKLV L+++ C  L+RLP     SKS++V++L  CRKI +  D  FEK PS LK +SL+V
Sbjct: 722  DKLVILDLEGCKILERLPRYISNSKSIEVMNLDSCRKIEQLFDNYFEKFPSHLKFESLKV 781

Query: 183  VSLSMCDNLEEITELSIASNLEVLDLSHCFNLRIIHECIGSLDKLVTLNLTDCSNLEKLP 242
            ++LS C NL+EIT+ SIASNLE+ DL  CF                              
Sbjct: 782  LNLSYCQNLKEITDFSIASNLEIFDLRGCF------------------------------ 841

Query: 243  NFSAALNLQELYLGNCTSLRTIHESIGSLNKLVTLDLRGCSNVEKLPSYLRLKFLDCLNL 302
                             SLRTIH+S+GSL++L+ L L  C  +E+LPS LRLK LD L+L
Sbjct: 842  -----------------SLRTIHKSVGSLDQLIALKLDFCHQLEELPSCLRLKSLDSLSL 901

Query: 303  SDYRRLEQLPEFDENMKSLRRMDLEFSAIKKLPLSIGNLTKLKFLSLRGCANLIALPSTI 362
            ++  ++EQLPEFDENMKSLR M+L+ +AI+KLP SI  L  L+ L L  C NLI+LPS I
Sbjct: 902  TNCYKIEQLPEFDENMKSLREMNLKGTAIRKLPTSIRYLIGLENLILSYCTNLISLPSEI 961

Query: 363  YLLESLERLCLRGCSKLFMFPQPSSLKVPQESLCSKLTFLDLKNCNILNVDFLETLSRVS 422
            +LL+SL+ L LR CS+L M P  SSL  PQ SLCS LT LDL+NCNI N DFLE LS   
Sbjct: 962  HLLKSLKELDLRECSRLDMLPSGSSLNFPQRSLCSNLTILDLQNCNISNSDFLENLSNFC 1021

Query: 423  TSLFELHLSGNKFSRLPSCLHNFTSLRELHLKNCKFLQEIPNLPQSIQIMDASGCESLVR 482
            T+L EL+LSGNKF  LPS L NFTSLR L L+NCKFL+ I  +P  ++ MDASGCE LV 
Sbjct: 1022 TTLKELNLSGNKFCCLPS-LKNFTSLRLLELRNCKFLRNIVKIPHCLKRMDASGCELLVI 1081

Query: 483  SPDNIADII----DYEFDSSKREFILMNSEIPELFSHQTRISSTSFNIQHHSDK-GRALA 542
            SPD IAD++    D +  + KRE I+  SEIP+  ++QT  SS SF+ QH+SD    AL 
Sbjct: 1082 SPDYIADMMFRNQDLKLRNFKRELIVTYSEIPKFCNNQTTESSISFSFQHNSDMIIPALV 1141

Query: 543  ACVVFKVNGYSDKAIAFISYHVLIDGKIWDSFWRRLFPT------STSEYMWLV-TTPLK 602
             CVVFKV+  S  A AFI + VL DG+      + + PT      S SE+M L+ T P +
Sbjct: 1142 VCVVFKVDADSFVAEAFIHFQVLFDGQ------KLMMPTMESWCGSKSEHMLLLRTPPSQ 1187

Query: 603  FSSLGINDRHEIKVWFEVYKIYN--AIVTIRSQGVHVIDGL 627
               L  N+RH+I+V F+V + YN  A V IRS GV+V+D L
Sbjct: 1202 LICLNENNRHKIEVSFQV-RNYNKKAKVIIRSLGVYVVDDL 1187

BLAST of Tan0000012.3 vs. ExPASy TrEMBL
Match: A0A0A0LWV1 (TIR domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G334920 PE=4 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 1.0e-136
Identity = 320/641 (49.92%), Postives = 408/641 (63.65%), Query Frame = 0

Query: 3    RSLEFLNLRGCEKLQEIPDLSIASNLKKLDLNGCINLRIIHESDFSLDKLVILNLGNCAN 62
            + L+ ++L     L+E PD S A NL+KL L  C  L++IH S  SL KLV L+L  C N
Sbjct: 636  KMLKHVDLSYWRLLEETPDFSAALNLEKLYLLSCKRLKMIHGSVASLSKLVTLDLEGCEN 695

Query: 63   LEKVP-SYLMSKSLEFLNLRKCKKLKEIYDFSAASNIQRVYLGECISLRIIHE-FVGRSL 122
            LEK+P S+LM KSLE LNL  C KLKEI D SA+SN++ ++L EC  LRIIH+  VGR L
Sbjct: 696  LEKLPSSFLMLKSLEVLNLSGCIKLKEIPDLSASSNLKELHLRECYHLRIIHDSAVGRFL 755

Query: 123  DKLVALEIKYCYNLKRLPCCFM-SKSLQVLDLTLCRKIIKFPDFSFEKLPSPLKLKSLRV 182
            DKLV L+++ C  L+RLP     SKS++V++L  CRKI +  D  FEK PS LK +SL+V
Sbjct: 756  DKLVILDLEGCKILERLPRYISNSKSIEVMNLDSCRKIEQLFDNYFEKFPSHLKFESLKV 815

Query: 183  VSLSMCDNLEEITELSIASNLEVLDLSHCFNLRIIHECIGSLDKLVTLNLTDCSNLEKLP 242
            ++LS C NL+EIT+ SIASNLE+ DL  CF                              
Sbjct: 816  LNLSYCQNLKEITDFSIASNLEIFDLRGCF------------------------------ 875

Query: 243  NFSAALNLQELYLGNCTSLRTIHESIGSLNKLVTLDLRGCSNVEKLPSYLRLKFLDCLNL 302
                             SLRTIH+S+GSL++L+ L L  C  +E+LPS LRLK LD L+L
Sbjct: 876  -----------------SLRTIHKSVGSLDQLIALKLDFCHQLEELPSCLRLKSLDSLSL 935

Query: 303  SDYRRLEQLPEFDENMKSLRRMDLEFSAIKKLPLSIGNLTKLKFLSLRGCANLIALPSTI 362
            ++  ++EQLPEFDENMKSLR M+L+ +AI+KLP SI  L  L+ L L  C NLI+LPS I
Sbjct: 936  TNCYKIEQLPEFDENMKSLREMNLKGTAIRKLPTSIRYLIGLENLILSYCTNLISLPSEI 995

Query: 363  YLLESLERLCLRGCSKLFMFPQPSSLKVPQESLCSKLTFLDLKNCNILNVDFLETLSRVS 422
            +LL+SL+ L LR CS+L M P  SSL  PQ SLCS LT LDL+NCNI N DFLE LS   
Sbjct: 996  HLLKSLKELDLRECSRLDMLPSGSSLNFPQRSLCSNLTILDLQNCNISNSDFLENLSNFC 1055

Query: 423  TSLFELHLSGNKFSRLPSCLHNFTSLRELHLKNCKFLQEIPNLPQSIQIMDASGCESLVR 482
            T+L EL+LSGNKF  LPS L NFTSLR L L+NCKFL+ I  +P  ++ MDASGCE LV 
Sbjct: 1056 TTLKELNLSGNKFCCLPS-LKNFTSLRLLELRNCKFLRNIVKIPHCLKRMDASGCELLVI 1115

Query: 483  SPDNIADII----DYEFDSSKREFILMNSEIPELFSHQTRISSTSFNIQHHSDK-GRALA 542
            SPD IAD++    D +  + KRE I+  SEIP+  ++QT  SS SF+ QH+SD    AL 
Sbjct: 1116 SPDYIADMMFRNQDLKLRNFKRELIVTYSEIPKFCNNQTTESSISFSFQHNSDMIIPALV 1175

Query: 543  ACVVFKVNGYSDKAIAFISYHVLIDGKIWDSFWRRLFPT------STSEYMWLV-TTPLK 602
             CVVFKV+  S  A AFI + VL DG+      + + PT      S SE+M L+ T P +
Sbjct: 1176 VCVVFKVDADSFVAEAFIHFQVLFDGQ------KLMMPTMESWCGSKSEHMLLLRTPPSQ 1221

Query: 603  FSSLGINDRHEIKVWFEVYKIYN--AIVTIRSQGVHVIDGL 627
               L  N+RH+I+V F+V + YN  A V IRS GV+V+D L
Sbjct: 1236 LICLNENNRHKIEVSFQV-RNYNKKAKVIIRSLGVYVVDDL 1221

BLAST of Tan0000012.3 vs. ExPASy TrEMBL
Match: A0A5D3C521 (TMV resistance protein N OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold163G001460 PE=4 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 1.8e-136
Identity = 314/634 (49.53%), Postives = 405/634 (63.88%), Query Frame = 0

Query: 3    RSLEFLNLRGCEKLQEIPDLSIASNLKKLDLNGCINLRIIHESDFSLDKLVILNLGNCAN 62
            + L+ ++L     L+E PD S+A NL+KL L  C  L +IH S  SL KLV L+L  C N
Sbjct: 610  KMLKHVDLSYWRLLEETPDFSVALNLEKLYLRSCKRLEMIHGSIASLSKLVTLDLEGCEN 669

Query: 63   LEKVP-SYLMSKSLEFLNLRKCKKLKEIYDFSAASNIQRVYLGECISLRIIHEFVGRSLD 122
            LEK+P S+LM KSLE LNL  C KLKEI D SA+S+++ ++L EC +LRIIH+ VGR LD
Sbjct: 670  LEKLPSSFLMLKSLEVLNLSGCIKLKEIPDLSASSSLKELHLRECYNLRIIHDSVGRFLD 729

Query: 123  KLVALEIKYCYNLKRLPCCFMSK--SLQVLDLTLCRKIIKFPDFSFEKLPSPLKLKSLRV 182
            KLV L+ + C NL+RLP  ++SK  S++VL+L  CRKI +  D  FEK P+ LK +SL+V
Sbjct: 730  KLVILDFEGCRNLERLP-RYISKSGSIEVLNLDSCRKIEQIFDNYFEKFPNHLKYESLKV 789

Query: 183  VSLSMCDNLEEITELSIASNLEVLDLSHCFNLRIIHECIGSLDKLVTLNLTDCSNLEKLP 242
            ++LS C NL+ IT+ S ASNLE+LDL  CF                              
Sbjct: 790  LNLSYCQNLKGITDFSFASNLEILDLRGCF------------------------------ 849

Query: 243  NFSAALNLQELYLGNCTSLRTIHESIGSLNKLVTLDLRGCSNVEKLPSYLRLKFLDCLNL 302
                             SLRTIHES+GSL+KL+ L L  C  +E+LPS LRLK LD L+L
Sbjct: 850  -----------------SLRTIHESVGSLDKLIALKLDSCHLLEELPSCLRLKSLDSLSL 909

Query: 303  SDYRRLEQLPEFDENMKSLRRMDLEFSAIKKLPLSIGNLTKLKFLSLRGCANLIALPSTI 362
            ++  +LEQLPEFDENMKSLR M+L+ +AI+KLP SI  L  L+ L+L  C NLI+LPS I
Sbjct: 910  TNCYKLEQLPEFDENMKSLREMNLKGTAIRKLPSSIRYLIGLENLNLSYCTNLISLPSEI 969

Query: 363  YLLESLERLCLRGCSKLFMFPQPSSLKVPQESLCSKLTFLDLKNCNILNVDFLETLSRVS 422
            +LL+SL+ L L  CS+L MFP  SSL  PQ+SL S LT LDL+NCNI N DFLE LS   
Sbjct: 970  HLLKSLKELDLHECSRLDMFPSGSSLNFPQQSLFSNLTILDLQNCNISNTDFLENLSNFC 1029

Query: 423  TSLFELHLSGNKFSRLPSCLHNFTSLRELHLKNCKFLQEIPNLPQSIQIMDASGCESLVR 482
            T+L EL+LSGNKF  LPS L NFTSLR L L+NCKFL+ I  +P  +  +DASGCE  V 
Sbjct: 1030 TTLKELNLSGNKFCSLPS-LQNFTSLRHLELRNCKFLRNIVKIPHCLTRVDASGCELFVI 1089

Query: 483  SPDNIADII----DYEFDSSKREFILMNSEIPELFSHQTRISSTSFNIQHHSDK-GRALA 542
            SPD IAD++    D E  + KRE I+  S+IP+  ++QT  SSTSF+ Q +SD    AL 
Sbjct: 1090 SPDYIADMMFRNQDLELRNFKRELIVAYSKIPKFCNNQTTESSTSFSFQQNSDTIIPALV 1149

Query: 543  ACVVFKVNGYSDKAIAFISYHVLIDGKIWDSFWRRLFPTSTSEYMWLV-TTPLKFSSLGI 602
             CVVFKV+  S  A  FI + VLIDG++        +  S SE+M L+ T P +   L  
Sbjct: 1150 VCVVFKVDEDSCDAEGFIRFQVLIDGQMLMMPTMEGWCGSKSEHMLLLRTPPSQLIYLNE 1194

Query: 603  NDRHEIKVWFEVYKI-YNAIVTIRSQGVHVIDGL 627
            N+RH+I+V F+V+     A V IRS GV+V++ L
Sbjct: 1210 NNRHKIEVSFQVWNYKKKAKVVIRSLGVYVVNEL 1194

BLAST of Tan0000012.3 vs. ExPASy TrEMBL
Match: A0A6J1G4A0 (TMV resistance protein N-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111450603 PE=4 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 2.6e-132
Identity = 297/635 (46.77%), Postives = 377/635 (59.37%), Query Frame = 0

Query: 16   LQEIPDLSIASNLKKLDLNGCINLRIIHESDFSLDKLVILNLGNCANLEKVPSYLMSKSL 75
            L++IPD S +S L+KL LN C NLR I  S   L KLVIL+L +C+NL+K+P+YL  KSL
Sbjct: 533  LEKIPDFSSSSGLEKLYLNHCTNLRAIPMSVVFLSKLVILDLNHCSNLKKLPNYLRLKSL 592

Query: 76   EFLNLRKCKKLKEIYDFSAASNIQRVYLGECISLRIIHEFVGRSLDKLVALEIKYCYNLK 135
            + LNL  CKKL+E+ +FSAASN++ +YL EC +LR+IHE +G SLD+LV L+++ C NLK
Sbjct: 593  KVLNLNYCKKLEELPNFSAASNLENLYLKECTNLRMIHESIG-SLDELVTLDLEKCSNLK 652

Query: 136  RLPCCFMSKSLQVLDLTLCRKIIKFPDFSFEKLPSPLKLKSLRVVSLSMCDNLEEITELS 195
                                           KLP  L LKSL+                 
Sbjct: 653  -------------------------------KLPGYLSLKSLK----------------- 712

Query: 196  IASNLEVLDLSHCFNLRIIHECIGSLDKLVTLNLTDCSNLEKLPNFSAALNLQELYLGNC 255
                                           LNL  C  L+++P+FSAALNLQ LYL  C
Sbjct: 713  ------------------------------HLNLDHCKKLQEIPDFSAALNLQSLYLKKC 772

Query: 256  TSLRTIHESIGSLNKLVTLDLRGCSNVEKLPSYLRLKFLDCLNLSDYRRLEQLPEFDENM 315
            T+LR +HESIGSL+ LV LD+R C+ +EKLPSYL+LK L  L LS   +LE  P+  ENM
Sbjct: 773  TNLRVLHESIGSLDNLVVLDVRHCTKLEKLPSYLKLKSLRYLELSGCSKLEMFPKMAENM 832

Query: 316  KSLRRMDLEFSAIKKLPLSIGNLTKLKFLSLRGCANLIALPSTIYLLESLERLCLRGCSK 375
            KSL  + L+ + IKKLP SIGNLT+LK L+L GC NLI++P  IYLL++L+ L L GCS+
Sbjct: 833  KSLVLLHLDSTGIKKLPSSIGNLTELKVLNLDGCTNLISVPGIIYLLQNLKELHLGGCSR 892

Query: 376  LFMFP------------QPSSLKVPQESLCSKLTFLDLKNCNILNVDFLETLSRVSTSLF 435
              M P              +   +P   + S+LT LDL++CN+ N DFLE L  V+  L 
Sbjct: 893  FEMLPHKWDSPIHPLCSSSNHGNLPTNPMFSQLTLLDLQHCNLSNADFLEILCDVNPFLN 952

Query: 436  ELHLSGNKFSRLPSCLHNFTSLRELHLKNCKFLQEIPNLPQSIQIMDASGCESLVRSPDN 495
             L LS NKF  LPSCLH F SL  L L+NCKFL++IP LPQ IQ MDA+GCESLVRSPDN
Sbjct: 953  SLLLSKNKFCSLPSCLHKFMSLWNLQLRNCKFLRQIPILPQCIQRMDATGCESLVRSPDN 1012

Query: 496  IADII----DYEFDSSKREFILMNSEIPELFSHQTRISSTSFNIQHHSDKGRALAACVVF 555
            + DII    D       REFILMN EIPE FSHQT  +S   + +H  +  R LA    F
Sbjct: 1013 VLDIISSKQDITLGDFPREFILMNIEIPEWFSHQTISNSIKVSFRHDHNIERTLATSAKF 1072

Query: 556  KVNGYSDKAIAFISYHVLIDGKIWDSFWRRLFPTSTSEYMWLVTT--PLKFSSLGINDRH 615
            +V+G S +  A IS ++ I  ++  SF R+  P STSEY WLVTT  P   SSL  ND +
Sbjct: 1073 RVDGDSYEGEALISCNIFIGYRLQSSFMRKFAP-STSEYTWLVTTSSPTFSSSLVANDWN 1087

Query: 616  EIKVWFEVYKIYNAIVTIRSQGVHV---IDGLQAD 630
            ++ VWFEV K     VTIRS GVH+   +DG+Q D
Sbjct: 1133 DVIVWFEVVKRREVNVTIRSCGVHLTDEVDGIQND 1087

BLAST of Tan0000012.3 vs. ExPASy TrEMBL
Match: A0A6J1G421 (TMV resistance protein N-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450603 PE=4 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 2.6e-132
Identity = 297/635 (46.77%), Postives = 377/635 (59.37%), Query Frame = 0

Query: 16   LQEIPDLSIASNLKKLDLNGCINLRIIHESDFSLDKLVILNLGNCANLEKVPSYLMSKSL 75
            L++IPD S +S L+KL LN C NLR I  S   L KLVIL+L +C+NL+K+P+YL  KSL
Sbjct: 642  LEKIPDFSSSSGLEKLYLNHCTNLRAIPMSVVFLSKLVILDLNHCSNLKKLPNYLRLKSL 701

Query: 76   EFLNLRKCKKLKEIYDFSAASNIQRVYLGECISLRIIHEFVGRSLDKLVALEIKYCYNLK 135
            + LNL  CKKL+E+ +FSAASN++ +YL EC +LR+IHE +G SLD+LV L+++ C NLK
Sbjct: 702  KVLNLNYCKKLEELPNFSAASNLENLYLKECTNLRMIHESIG-SLDELVTLDLEKCSNLK 761

Query: 136  RLPCCFMSKSLQVLDLTLCRKIIKFPDFSFEKLPSPLKLKSLRVVSLSMCDNLEEITELS 195
                                           KLP  L LKSL+                 
Sbjct: 762  -------------------------------KLPGYLSLKSLK----------------- 821

Query: 196  IASNLEVLDLSHCFNLRIIHECIGSLDKLVTLNLTDCSNLEKLPNFSAALNLQELYLGNC 255
                                           LNL  C  L+++P+FSAALNLQ LYL  C
Sbjct: 822  ------------------------------HLNLDHCKKLQEIPDFSAALNLQSLYLKKC 881

Query: 256  TSLRTIHESIGSLNKLVTLDLRGCSNVEKLPSYLRLKFLDCLNLSDYRRLEQLPEFDENM 315
            T+LR +HESIGSL+ LV LD+R C+ +EKLPSYL+LK L  L LS   +LE  P+  ENM
Sbjct: 882  TNLRVLHESIGSLDNLVVLDVRHCTKLEKLPSYLKLKSLRYLELSGCSKLEMFPKMAENM 941

Query: 316  KSLRRMDLEFSAIKKLPLSIGNLTKLKFLSLRGCANLIALPSTIYLLESLERLCLRGCSK 375
            KSL  + L+ + IKKLP SIGNLT+LK L+L GC NLI++P  IYLL++L+ L L GCS+
Sbjct: 942  KSLVLLHLDSTGIKKLPSSIGNLTELKVLNLDGCTNLISVPGIIYLLQNLKELHLGGCSR 1001

Query: 376  LFMFP------------QPSSLKVPQESLCSKLTFLDLKNCNILNVDFLETLSRVSTSLF 435
              M P              +   +P   + S+LT LDL++CN+ N DFLE L  V+  L 
Sbjct: 1002 FEMLPHKWDSPIHPLCSSSNHGNLPTNPMFSQLTLLDLQHCNLSNADFLEILCDVNPFLN 1061

Query: 436  ELHLSGNKFSRLPSCLHNFTSLRELHLKNCKFLQEIPNLPQSIQIMDASGCESLVRSPDN 495
             L LS NKF  LPSCLH F SL  L L+NCKFL++IP LPQ IQ MDA+GCESLVRSPDN
Sbjct: 1062 SLLLSKNKFCSLPSCLHKFMSLWNLQLRNCKFLRQIPILPQCIQRMDATGCESLVRSPDN 1121

Query: 496  IADII----DYEFDSSKREFILMNSEIPELFSHQTRISSTSFNIQHHSDKGRALAACVVF 555
            + DII    D       REFILMN EIPE FSHQT  +S   + +H  +  R LA    F
Sbjct: 1122 VLDIISSKQDITLGDFPREFILMNIEIPEWFSHQTISNSIKVSFRHDHNIERTLATSAKF 1181

Query: 556  KVNGYSDKAIAFISYHVLIDGKIWDSFWRRLFPTSTSEYMWLVTT--PLKFSSLGINDRH 615
            +V+G S +  A IS ++ I  ++  SF R+  P STSEY WLVTT  P   SSL  ND +
Sbjct: 1182 RVDGDSYEGEALISCNIFIGYRLQSSFMRKFAP-STSEYTWLVTTSSPTFSSSLVANDWN 1196

Query: 616  EIKVWFEVYKIYNAIVTIRSQGVHV---IDGLQAD 630
            ++ VWFEV K     VTIRS GVH+   +DG+Q D
Sbjct: 1242 DVIVWFEVVKRREVNVTIRSCGVHLTDEVDGIQND 1196

BLAST of Tan0000012.3 vs. ExPASy TrEMBL
Match: A0A6J1G440 (TMV resistance protein N-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111450603 PE=4 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 2.6e-132
Identity = 297/635 (46.77%), Postives = 377/635 (59.37%), Query Frame = 0

Query: 16   LQEIPDLSIASNLKKLDLNGCINLRIIHESDFSLDKLVILNLGNCANLEKVPSYLMSKSL 75
            L++IPD S +S L+KL LN C NLR I  S   L KLVIL+L +C+NL+K+P+YL  KSL
Sbjct: 546  LEKIPDFSSSSGLEKLYLNHCTNLRAIPMSVVFLSKLVILDLNHCSNLKKLPNYLRLKSL 605

Query: 76   EFLNLRKCKKLKEIYDFSAASNIQRVYLGECISLRIIHEFVGRSLDKLVALEIKYCYNLK 135
            + LNL  CKKL+E+ +FSAASN++ +YL EC +LR+IHE +G SLD+LV L+++ C NLK
Sbjct: 606  KVLNLNYCKKLEELPNFSAASNLENLYLKECTNLRMIHESIG-SLDELVTLDLEKCSNLK 665

Query: 136  RLPCCFMSKSLQVLDLTLCRKIIKFPDFSFEKLPSPLKLKSLRVVSLSMCDNLEEITELS 195
                                           KLP  L LKSL+                 
Sbjct: 666  -------------------------------KLPGYLSLKSLK----------------- 725

Query: 196  IASNLEVLDLSHCFNLRIIHECIGSLDKLVTLNLTDCSNLEKLPNFSAALNLQELYLGNC 255
                                           LNL  C  L+++P+FSAALNLQ LYL  C
Sbjct: 726  ------------------------------HLNLDHCKKLQEIPDFSAALNLQSLYLKKC 785

Query: 256  TSLRTIHESIGSLNKLVTLDLRGCSNVEKLPSYLRLKFLDCLNLSDYRRLEQLPEFDENM 315
            T+LR +HESIGSL+ LV LD+R C+ +EKLPSYL+LK L  L LS   +LE  P+  ENM
Sbjct: 786  TNLRVLHESIGSLDNLVVLDVRHCTKLEKLPSYLKLKSLRYLELSGCSKLEMFPKMAENM 845

Query: 316  KSLRRMDLEFSAIKKLPLSIGNLTKLKFLSLRGCANLIALPSTIYLLESLERLCLRGCSK 375
            KSL  + L+ + IKKLP SIGNLT+LK L+L GC NLI++P  IYLL++L+ L L GCS+
Sbjct: 846  KSLVLLHLDSTGIKKLPSSIGNLTELKVLNLDGCTNLISVPGIIYLLQNLKELHLGGCSR 905

Query: 376  LFMFP------------QPSSLKVPQESLCSKLTFLDLKNCNILNVDFLETLSRVSTSLF 435
              M P              +   +P   + S+LT LDL++CN+ N DFLE L  V+  L 
Sbjct: 906  FEMLPHKWDSPIHPLCSSSNHGNLPTNPMFSQLTLLDLQHCNLSNADFLEILCDVNPFLN 965

Query: 436  ELHLSGNKFSRLPSCLHNFTSLRELHLKNCKFLQEIPNLPQSIQIMDASGCESLVRSPDN 495
             L LS NKF  LPSCLH F SL  L L+NCKFL++IP LPQ IQ MDA+GCESLVRSPDN
Sbjct: 966  SLLLSKNKFCSLPSCLHKFMSLWNLQLRNCKFLRQIPILPQCIQRMDATGCESLVRSPDN 1025

Query: 496  IADII----DYEFDSSKREFILMNSEIPELFSHQTRISSTSFNIQHHSDKGRALAACVVF 555
            + DII    D       REFILMN EIPE FSHQT  +S   + +H  +  R LA    F
Sbjct: 1026 VLDIISSKQDITLGDFPREFILMNIEIPEWFSHQTISNSIKVSFRHDHNIERTLATSAKF 1085

Query: 556  KVNGYSDKAIAFISYHVLIDGKIWDSFWRRLFPTSTSEYMWLVTT--PLKFSSLGINDRH 615
            +V+G S +  A IS ++ I  ++  SF R+  P STSEY WLVTT  P   SSL  ND +
Sbjct: 1086 RVDGDSYEGEALISCNIFIGYRLQSSFMRKFAP-STSEYTWLVTTSSPTFSSSLVANDWN 1100

Query: 616  EIKVWFEVYKIYNAIVTIRSQGVHV---IDGLQAD 630
            ++ VWFEV K     VTIRS GVH+   +DG+Q D
Sbjct: 1146 DVIVWFEVVKRREVNVTIRSCGVHLTDEVDGIQND 1100

BLAST of Tan0000012.3 vs. TAIR 10
Match: AT4G16890.1 (disease resistance protein (TIR-NBS-LRR class), putative )

HSP 1 Score: 202.2 bits (513), Expect = 1.3e-51
Identity = 166/513 (32.36%), Postives = 256/513 (49.90%), Query Frame = 0

Query: 2    LRSLEFLNLRGCEKLQEIPDLSIASNLKKLDLNGCINLRIIHESDFSLDKLVILNLGNCA 61
            L SL+ +NLR    L+EIPDLS+A NL++LDL GC +L  +  S  +  KL+ L++ +C 
Sbjct: 612  LGSLKEMNLRYSNNLKEIPDLSLAINLEELDLVGCKSLVTLPSSIQNATKLIYLDMSDCK 671

Query: 62   NLEKVPSYLMSKSLEFLNLRKCKKLKEI---------YDFSAASNIQRVYLGECISLRII 121
             LE  P+ L  +SLE+LNL  C  L+            DF    N   + + +C      
Sbjct: 672  KLESFPTDLNLESLEYLNLTGCPNLRNFPAIKMGCSDVDFPEGRN--EIVVEDC----FW 731

Query: 122  HEFVGRSLDKLVALEIKYCYNLKRLPCCFMSKSLQVLDLTLCRKIIKFPDFSFEKLPSPL 181
            ++ +   LD L  L        + +PC F  + L  L++           +  EKL   +
Sbjct: 732  NKNLPAGLDYLDCL-------TRCMPCEFRPEQLAFLNVR---------GYKHEKLWEGI 791

Query: 182  K-LKSLRVVSLSMCDNLEEITELSIASNLEVLDLSHCFNLRIIHECIGSLDKLVTLNLTD 241
            + L SL  + LS  +NL EI +LS A+ LE L L++C +L  +   IG+L +LV L + +
Sbjct: 792  QSLGSLEGMDLSESENLTEIPDLSKATKLESLILNNCKSLVTLPSTIGNLHRLVRLEMKE 851

Query: 242  CSNLEKLPNFSAALNLQELYLGNCTSLRT--------------------IHESIGSLNKL 301
            C+ LE LP      +L+ L L  C+SLR+                    I  +IG+L++L
Sbjct: 852  CTGLEVLPTDVNLSSLETLDLSGCSSLRSFPLISTNIVWLYLENTAIEEIPSTIGNLHRL 911

Query: 302  VTLDLRGCSNVEKLPSYLRLKFLDCLNLSDYRRLEQLPEFDENMKSLRRMDLEFSAIKKL 361
            V L+++ C+ +E LP+ + L  L+ L+LS    L   P   E++K L    LE +AI+++
Sbjct: 912  VRLEMKKCTGLEVLPTDVNLSSLETLDLSGCSSLRSFPLISESIKWLY---LENTAIEEI 971

Query: 362  PLSIGNLTKLKFLSLRGCANLIALPSTIYLLESLERLCLRGCSKLFMFPQPSSLKVPQES 421
            P  +   T LK L L  C +L+ LP+TI  L+ L    ++ C+ L + P   +L      
Sbjct: 972  P-DLSKATNLKNLKLNNCKSLVTLPTTIGNLQKLVSFEMKECTGLEVLPIDVNL------ 1031

Query: 422  LCSKLTFLDLKNCNILNVDFLETLSRVSTSLFELHLSGNKFSRLPSCLHNFTSLRELHLK 481
              S L  LDL  C+      L T   +ST++  L+L       +PS + N   L +L +K
Sbjct: 1032 --SSLMILDLSGCS-----SLRTFPLISTNIVWLYLENTAIEEIPSTIGNLHRLVKLEMK 1084

BLAST of Tan0000012.3 vs. TAIR 10
Match: AT1G69550.1 (disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 200.3 bits (508), Expect = 5.0e-51
Identity = 180/520 (34.62%), Postives = 276/520 (53.08%), Query Frame = 0

Query: 10   LRGCEKLQEIP-DLSIASNLKKLDLNGCINLRIIHESDFSLDKLVILNLGNCANLEKVPS 69
            L  C  L E+P  +  A+N+K LD+ GC +L  +  S  +L  L  L+L  C++L ++PS
Sbjct: 723  LSDCSSLIELPSSIGNATNIKSLDIQGCSSLLKLPSSIGNLITLPRLDLMGCSSLVELPS 782

Query: 70   YLMSK-SLEFLNLRKCKKLKEI-YDFSAASNIQRVYLGECISLRIIHEFVGRSLDKLVAL 129
             + +  +L  L+L  C  L E+        N++  Y   C SL  +   +G +L  L  L
Sbjct: 783  SIGNLINLPRLDLMGCSSLVELPSSIGNLINLEAFYFHGCSSLLELPSSIG-NLISLKIL 842

Query: 130  EIKYCYNLKRLPCCFMSK-SLQVLDLTLCRKIIKFPDFSFEKLPSPLKLKSLRVVSLSMC 189
             +K   +L  +P    +  +L++L+L+ C  +++ P        S   L +L+ + LS C
Sbjct: 843  YLKRISSLVEIPSSIGNLINLKLLNLSGCSSLVELPS-------SIGNLINLKKLDLSGC 902

Query: 190  DNLEEITELSIAS--NLEVLDLSHCFNLRIIHECIGSLDKLVTLNLTDCSNLEKLP-NFS 249
             +L E+  LSI +  NL+ L LS C +L  +   IG+L  L TLNL++CS+L +LP +  
Sbjct: 903  SSLVEL-PLSIGNLINLQELYLSECSSLVELPSSIGNLINLKTLNLSECSSLVELPSSIG 962

Query: 250  AALNLQELYLGNCTSLRTIHESIGSLNKLVTLDLRGCSNVEKLP-SYLRLKFLDCLNLSD 309
              +NLQELYL  C+SL  +  SIG+L  L  LDL GCS++ +LP S   L  L  LNLS+
Sbjct: 963  NLINLQELYLSECSSLVELPSSIGNLINLKKLDLSGCSSLVELPLSIGNLINLKTLNLSE 1022

Query: 310  YRRLEQLPEFDENMKSLRRMDL-EFSAIKKLPLSIGNLTKLKFLSLRGCANLIALPSTIY 369
               L +LP    N+ +L+ + L E S++ +LP SIGNL  LK L L GC++L+ LP +I 
Sbjct: 1023 CSSLVELPSSIGNLINLQELYLSECSSLVELPSSIGNLINLKKLDLSGCSSLVELPLSIG 1082

Query: 370  LLESLERLCLRGCSKLFMFPQP---SSLKVPQESLCSKLTFLDLKNCNILNVDFLETLSR 429
             L +L+ L L GCS L   P      +LK    S CS L  L     N++N         
Sbjct: 1083 NLINLKTLNLSGCSSLVELPSSIGNLNLKKLDLSGCSSLVELPSSIGNLIN--------- 1142

Query: 430  VSTSLFELHLSG-NKFSRLPSCLHNFTSLRELHLKNCKFLQEIP----NLPQSIQIMDAS 489
                L +L LSG +    LP  + N  +L+EL+L  C  L E+P    NL  ++Q +  S
Sbjct: 1143 ----LKKLDLSGCSSLVELPLSIGNLINLQELYLSECSSLVELPSSIGNL-INLQELYLS 1202

Query: 490  GCESLVRSPDNIADIIDY-EFDSSKREFILMNSEIPELFS 512
             C SLV  P +I ++I+  + D +K   ++   ++P+  S
Sbjct: 1203 ECSSLVELPSSIGNLINLKKLDLNKCTKLVSLPQLPDSLS 1219

BLAST of Tan0000012.3 vs. TAIR 10
Match: AT3G25510.1 (disease resistance protein (TIR-NBS-LRR class), putative )

HSP 1 Score: 183.7 bits (465), Expect = 4.9e-46
Identity = 169/556 (30.40%), Postives = 267/556 (48.02%), Query Frame = 0

Query: 40   RIIHESDFSLDKLVILNL--GNCANLEKVPSYLMSKSLEFLNLRKCKKLKEIYDFSAASN 99
            R+   S F+ + LV LN+    C  L +    L  ++L++++L     LKE+ D S A+N
Sbjct: 631  RLCLPSTFNPEFLVELNMPSSTCHTLWEGSKAL--RNLKWMDLSYSISLKELPDLSTATN 690

Query: 100  IQRVYLGECISLRIIHEFVGRSLDKLVALEIKYCYNLKRLPCCFMS-KSLQVLDLTLCRK 159
            ++ + L  C+SL  +   VG+ L KL  L +  C ++  LP    +   LQ LDL  C  
Sbjct: 691  LEELILKYCVSLVKVPSCVGK-LGKLQVLCLHGCTSILELPSFTKNVTGLQSLDLNECSS 750

Query: 160  IIKFPD---------------FSFEKLP-SPLKLKSLRVVSLSMCDNLEEITELSIASNL 219
            +++ P                    KLP S +K  +L+   L+ C +L E+  +  A+NL
Sbjct: 751  LVELPSSIGNAINLQNLDLGCLRLLKLPLSIVKFTNLKKFILNGCSSLVELPFMGNATNL 810

Query: 220  EVLDLSHCFNLRIIHECIGSLDKLVTLNLTDCSNLEKLPNF-SAALNLQELYLGNCTSLR 279
            + LDL +C +L  +   IG+   L  L+L++CS+L KLP+F   A NL+ L L  C+SL 
Sbjct: 811  QNLDLGNCSSLVELPSSIGNAINLQNLDLSNCSSLVKLPSFIGNATNLEILDLRKCSSLV 870

Query: 280  TIHESIGSLNKLVTLDLRGCSNVEKLPSYL-RLKFLDCLNLSDYRRLEQLPEFDENMKSL 339
             I  SIG +  L  LDL GCS++ +LPS +  +  L  LNL +   L +LP    +  +L
Sbjct: 871  EIPTSIGHVTNLWRLDLSGCSSLVELPSSVGNISELQVLNLHNCSNLVKLPSSFGHATNL 930

Query: 340  RRMDLE-FSAIKKLPLSIGNLTKLKFLSLRGCANLIALPSTIYLLESLERLCLRGCSKLF 399
             R+DL   S++ +LP SIGN+T L+ L+L  C+NL+ LPS+I  L  L  L L  C KL 
Sbjct: 931  WRLDLSGCSSLVELPSSIGNITNLQELNLCNCSNLVKLPSSIGNLHLLFTLSLARCQKLE 990

Query: 400  MFPQPSSLKVPQESLCSKLTFLDLKNCNILNVDFLETLSRVSTSLFELHLSGNKFSRLPS 459
              P   +LK         L  LDL +C+       ++   +ST++  L+L G     +PS
Sbjct: 991  ALPSNINLK--------SLERLDLTDCS-----QFKSFPEISTNIECLYLDGTAVEEVPS 1050

Query: 460  CLHNFTSLRELH-------------------------------------------LKNCK 514
             + +++ L  LH                                           L  C+
Sbjct: 1051 SIKSWSRLTVLHMSYFEKLKEFSHVLDIITWLEFGEDIQEVAPWIKEISRLHGLRLYKCR 1110

BLAST of Tan0000012.3 vs. TAIR 10
Match: AT4G16950.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 178.3 bits (451), Expect = 2.0e-44
Identity = 164/562 (29.18%), Postives = 258/562 (45.91%), Query Frame = 0

Query: 2    LRSLEFLNLRGCEKLQEIPDLSIASNLKKLDLNGC-------------INLRIIH----- 61
            L SL+ +NL   + L+EIPDLS A NL++LDL GC             I LR +H     
Sbjct: 618  LGSLKKMNLLCSKNLKEIPDLSNARNLEELDLEGCESLVTLPSSIQNAIKLRKLHCSGVI 677

Query: 62   -------------------------------------------------ESDFSLDKLVI 121
                                                              S+F ++ LV 
Sbjct: 678  LIDLKSLEGMCNLEYLSVDCSRVEGTQGIVYFPSKLRLLLWNNCPLKRLHSNFKVEYLVK 737

Query: 122  LNLGNCANLEKVPSYLMSKS-LEFLNLRKCKKLKEIYDFSAASNIQRVYLGECISLRIIH 181
            L + N ++LEK+         L+ + LR  K LKEI D S A N++ V + +C SL    
Sbjct: 738  LRMEN-SDLEKLWDGTQPLGRLKQMFLRGSKYLKEIPDLSLAINLEEVDICKCESLVTFP 797

Query: 182  EFVGRSLDKLVALEIKYCYNLKRLPCCFMSKSLQVLDLTLCRKIIKFP-------DFSFE 241
              +  ++ KL+ L+I  C  L+  P     +SL+ L+LT C  +  FP       D  F 
Sbjct: 798  SSMQNAI-KLIYLDISDCKKLESFPTDLNLESLEYLNLTGCPNLRNFPAIKMGCSDVDFP 857

Query: 242  KLPSPLKLKSL-----RVVSLSMCDNLEEITELSIASNLEVLDLSHCFNLRIIHECIGSL 301
            +  + + ++           L   D L             V     C+    + E I SL
Sbjct: 858  EGRNEIVVEDCFWNKNLPAGLDYLDCLMRCMPCEFRPEYLVFLNVRCYKHEKLWEGIQSL 917

Query: 302  DKLVTLNLTDCSNLEKLPNFSAALNLQELYLGNCTSLRTIHESIGSLNKLVTLDLRGCSN 361
              L  ++L++  NL ++P+ S A NL+ LYL NC SL T+  +IG+L KLV L+++ C+ 
Sbjct: 918  GSLEEMDLSESENLTEIPDLSKATNLKHLYLNNCKSLVTLPSTIGNLQKLVRLEMKECTG 977

Query: 362  VEKLPSYLRLKFLDCLNLSDYRRLEQLPEFDENMKSLRRMDLEFSAIKKLPLSIGNLTKL 421
            +E LP+ + L  L+ L+LS    L   P      KS++ + LE +AI+++ L +   TKL
Sbjct: 978  LEVLPTDVNLSSLETLDLSGCSSLRTFPLIS---KSIKWLYLENTAIEEI-LDLSKATKL 1037

Query: 422  KFLSLRGCANLIALPSTIYLLESLERLCLRGCSKLFMFPQPSSLKVPQESLCSKLTFLDL 481
            + L L  C +L+ LPSTI  L++L RL ++ C+ L + P   +L        S L  LDL
Sbjct: 1038 ESLILNNCKSLVTLPSTIGNLQNLRRLYMKRCTGLEVLPTDVNL--------SSLGILDL 1097

BLAST of Tan0000012.3 vs. TAIR 10
Match: AT4G16950.2 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 178.3 bits (451), Expect = 2.0e-44
Identity = 164/562 (29.18%), Postives = 258/562 (45.91%), Query Frame = 0

Query: 2    LRSLEFLNLRGCEKLQEIPDLSIASNLKKLDLNGC-------------INLRIIH----- 61
            L SL+ +NL   + L+EIPDLS A NL++LDL GC             I LR +H     
Sbjct: 618  LGSLKKMNLLCSKNLKEIPDLSNARNLEELDLEGCESLVTLPSSIQNAIKLRKLHCSGVI 677

Query: 62   -------------------------------------------------ESDFSLDKLVI 121
                                                              S+F ++ LV 
Sbjct: 678  LIDLKSLEGMCNLEYLSVDCSRVEGTQGIVYFPSKLRLLLWNNCPLKRLHSNFKVEYLVK 737

Query: 122  LNLGNCANLEKVPSYLMSKS-LEFLNLRKCKKLKEIYDFSAASNIQRVYLGECISLRIIH 181
            L + N ++LEK+         L+ + LR  K LKEI D S A N++ V + +C SL    
Sbjct: 738  LRMEN-SDLEKLWDGTQPLGRLKQMFLRGSKYLKEIPDLSLAINLEEVDICKCESLVTFP 797

Query: 182  EFVGRSLDKLVALEIKYCYNLKRLPCCFMSKSLQVLDLTLCRKIIKFP-------DFSFE 241
              +  ++ KL+ L+I  C  L+  P     +SL+ L+LT C  +  FP       D  F 
Sbjct: 798  SSMQNAI-KLIYLDISDCKKLESFPTDLNLESLEYLNLTGCPNLRNFPAIKMGCSDVDFP 857

Query: 242  KLPSPLKLKSL-----RVVSLSMCDNLEEITELSIASNLEVLDLSHCFNLRIIHECIGSL 301
            +  + + ++           L   D L             V     C+    + E I SL
Sbjct: 858  EGRNEIVVEDCFWNKNLPAGLDYLDCLMRCMPCEFRPEYLVFLNVRCYKHEKLWEGIQSL 917

Query: 302  DKLVTLNLTDCSNLEKLPNFSAALNLQELYLGNCTSLRTIHESIGSLNKLVTLDLRGCSN 361
              L  ++L++  NL ++P+ S A NL+ LYL NC SL T+  +IG+L KLV L+++ C+ 
Sbjct: 918  GSLEEMDLSESENLTEIPDLSKATNLKHLYLNNCKSLVTLPSTIGNLQKLVRLEMKECTG 977

Query: 362  VEKLPSYLRLKFLDCLNLSDYRRLEQLPEFDENMKSLRRMDLEFSAIKKLPLSIGNLTKL 421
            +E LP+ + L  L+ L+LS    L   P      KS++ + LE +AI+++ L +   TKL
Sbjct: 978  LEVLPTDVNLSSLETLDLSGCSSLRTFPLIS---KSIKWLYLENTAIEEI-LDLSKATKL 1037

Query: 422  KFLSLRGCANLIALPSTIYLLESLERLCLRGCSKLFMFPQPSSLKVPQESLCSKLTFLDL 481
            + L L  C +L+ LPSTI  L++L RL ++ C+ L + P   +L        S L  LDL
Sbjct: 1038 ESLILNNCKSLVTLPSTIGNLQNLRRLYMKRCTGLEVLPTDVNL--------SSLGILDL 1097