Spg022725.1 (mRNA) Sponge gourd (cylindrica) v1

BLAST of Spg022725.1 vs. NCBI nr
Match: KAG6589933.1 (Transmembrane protein 234-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 206.5 bits (524), Expect = 3.1e-49
Identity = 109/126 (86.51%), Postives = 118/126 (93.65%), Query Frame = 0

Query: 1   MIGDVEKMVAIGLVWGATNALMRRGALLWDQALKSSSNSTHTSTKLLSSLRRWLKLLSIW 60
           MIGDVEKMVAIG+VWGATNALMRRGALLWDQALKSSSN+T +S KLLSSLRRWLK+LSIW
Sbjct: 1   MIGDVEKMVAIGIVWGATNALMRRGALLWDQALKSSSNATRSSNKLLSSLRRWLKVLSIW 60

Query: 61  QYTVPFLLNLTASATFFAILSDAPISLAVPVTNATTFAATAVFGMLLGEDTRVG-GLVGI 120
           QYTVPFL+NLTASATFFAILSDAPISLAVPVTNATTFAATAVFGMLLGE+TRVG  L G 
Sbjct: 61  QYTVPFLVNLTASATFFAILSDAPISLAVPVTNATTFAATAVFGMLLGEETRVGYALFGF 120

Query: 121 WKIEPN 126
           ++ + N
Sbjct: 121 YRCDCN 126

BLAST of Spg022725.1 vs. NCBI nr
Match: XP_022961299.1 (transmembrane protein 234 homolog [Cucurbita moschata] >XP_022961300.1 transmembrane protein 234 homolog [Cucurbita moschata] >XP_022961301.1 transmembrane protein 234 homolog [Cucurbita moschata] >XP_022961303.1 transmembrane protein 234 homolog [Cucurbita moschata])

HSP 1 Score: 204.9 bits (520), Expect = 9.0e-49
Identity = 107/114 (93.86%), Postives = 112/114 (98.25%), Query Frame = 0

Query: 1   MIGDVEKMVAIGLVWGATNALMRRGALLWDQALKSSSNSTHTSTKLLSSLRRWLKLLSIW 60
           MIGDVEKMVAIG+VWGATNALMRRGALLWDQALKSSSN+T +S KLLSSLRRWLKLLSIW
Sbjct: 1   MIGDVEKMVAIGIVWGATNALMRRGALLWDQALKSSSNATRSSNKLLSSLRRWLKLLSIW 60

Query: 61  QYTVPFLLNLTASATFFAILSDAPISLAVPVTNATTFAATAVFGMLLGEDTRVG 115
           QYTVPFL+NLTASATFFAILSDAPISLAVPVTNATTFAATAVFGMLLGE+TRVG
Sbjct: 61  QYTVPFLVNLTASATFFAILSDAPISLAVPVTNATTFAATAVFGMLLGEETRVG 114

BLAST of Spg022725.1 vs. NCBI nr
Match: XP_023516356.1 (transmembrane protein 234 homolog isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 203.8 bits (517), Expect = 2.0e-48
Identity = 106/114 (92.98%), Postives = 112/114 (98.25%), Query Frame = 0

Query: 1   MIGDVEKMVAIGLVWGATNALMRRGALLWDQALKSSSNSTHTSTKLLSSLRRWLKLLSIW 60
           MIGDVEKMVAIG+VWGATNALMRRGALLWDQALKSSSN+T +S KLLSSLRRWLKLLSIW
Sbjct: 1   MIGDVEKMVAIGMVWGATNALMRRGALLWDQALKSSSNATRSSNKLLSSLRRWLKLLSIW 60

Query: 61  QYTVPFLLNLTASATFFAILSDAPISLAVPVTNATTFAATAVFGMLLGEDTRVG 115
           QYT+PFL+NLTASATFFAILSDAPISLAVPVTNATTFAATAVFGMLLGE+TRVG
Sbjct: 61  QYTLPFLVNLTASATFFAILSDAPISLAVPVTNATTFAATAVFGMLLGEETRVG 114

BLAST of Spg022725.1 vs. NCBI nr
Match: XP_023516357.1 (transmembrane protein 234 homolog isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 203.8 bits (517), Expect = 2.0e-48
Identity = 106/114 (92.98%), Postives = 112/114 (98.25%), Query Frame = 0

Query: 1   MIGDVEKMVAIGLVWGATNALMRRGALLWDQALKSSSNSTHTSTKLLSSLRRWLKLLSIW 60
           MIGDVEKMVAIG+VWGATNALMRRGALLWDQALKSSSN+T +S KLLSSLRRWLKLLSIW
Sbjct: 1   MIGDVEKMVAIGMVWGATNALMRRGALLWDQALKSSSNATRSSNKLLSSLRRWLKLLSIW 60

Query: 61  QYTVPFLLNLTASATFFAILSDAPISLAVPVTNATTFAATAVFGMLLGEDTRVG 115
           QYT+PFL+NLTASATFFAILSDAPISLAVPVTNATTFAATAVFGMLLGE+TRVG
Sbjct: 61  QYTLPFLVNLTASATFFAILSDAPISLAVPVTNATTFAATAVFGMLLGEETRVG 114

BLAST of Spg022725.1 vs. NCBI nr
Match: KAG7023602.1 (Transmembrane protein-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 202.2 bits (513), Expect = 5.8e-48
Identity = 106/114 (92.98%), Postives = 111/114 (97.37%), Query Frame = 0

Query: 1   MIGDVEKMVAIGLVWGATNALMRRGALLWDQALKSSSNSTHTSTKLLSSLRRWLKLLSIW 60
           MIG VEKMVAIG+VWGATNALMRRGALLWDQALKSSSN+T +S KLLSSLRRWLKLLSIW
Sbjct: 1   MIGGVEKMVAIGIVWGATNALMRRGALLWDQALKSSSNATRSSNKLLSSLRRWLKLLSIW 60

Query: 61  QYTVPFLLNLTASATFFAILSDAPISLAVPVTNATTFAATAVFGMLLGEDTRVG 115
           QYTVPFL+NLTASATFFAILSDAPISLAVPVTNATTFAATAVFGMLLGE+TRVG
Sbjct: 61  QYTVPFLVNLTASATFFAILSDAPISLAVPVTNATTFAATAVFGMLLGEETRVG 114

BLAST of Spg022725.1 vs. ExPASy Swiss-Prot
Match: Q54ZG7 (Transmembrane protein 234 homolog OS=Dictyostelium discoideum OX=44689 GN=DDB_G0277575 PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 3.2e-09
Identity = 36/106 (33.96%), Postives = 58/106 (54.72%), Query Frame = 0

Query: 4   DVEKMVAIGLVWGATNALMRRGALLWDQALKSSSNSTHTSTKLLSSLRRWLKLLSIWQYT 63
           ++  ++ +G +WG TN L++RG+    + +         S  +   +  W +      YT
Sbjct: 5   NIISLLLVGFIWGGTNPLIKRGS----EGVSKVKKDNFLSQIIYEFVYLWTRP----SYT 64

Query: 64  VPFLLNLTASATFFAILSDAPISLAVPVTNATTFAATAVFGMLLGE 110
           +P L+NL+ S  FF  LS   ISL VP++N+ TF  T++ GMLLGE
Sbjct: 65  IPMLINLSGSVVFFYTLSKVDISLVVPISNSLTFLFTSLMGMLLGE 102

BLAST of Spg022725.1 vs. ExPASy Swiss-Prot
Match: A0NGI1 (Transmembrane protein 234 homolog OS=Anopheles gambiae OX=7165 GN=AGAP012180 PE=3 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 2.6e-06
Identity = 41/121 (33.88%), Postives = 65/121 (53.72%), Query Frame = 0

Query: 5   VEKMVAIGLVWGATNALMRRGALLWDQALKSSSNSTHTSTKLLSSLRRWLK---LLSIWQ 64
           V  ++ + ++WGATN  ++RG++ +++ LK+ S         L  L  WL+   L++ WQ
Sbjct: 17  VLSILLVAIMWGATNPFIKRGSIGYNE-LKADSK--------LGQL--WLEVRFLITRWQ 76

Query: 65  YTVPFLLNLTASATFFAILSDAPISLAVPVTNATTFAATAVFGMLLGEDTRVGGLVGIWK 123
           Y +P ++N   S  +   L    +SL VP+ N+ TF  TA+   LLGE  R  G    WK
Sbjct: 77  YLLPLVINQLGSIVYVLTLQRTELSLTVPMANSLTFVFTAITARLLGE--RQSG----WK 120

BLAST of Spg022725.1 vs. ExPASy Swiss-Prot
Match: Q8R1E7 (Transmembrane protein 234 OS=Mus musculus OX=10090 GN=Tmem234 PE=1 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 1.4e-04
Identity = 33/113 (29.20%), Postives = 53/113 (46.90%), Query Frame = 0

Query: 3   GDVEKMVAIGLVWGATNALMRRGALLWDQALKSSSNSTHTSTKLLSSLRRWLKLLSIWQY 62
           G V  +V +  +WG T  L++R +        S        T     L+    L    +Y
Sbjct: 6   GQVLALVLVAALWGGTQPLLKRAS--------SGLEQVRERTWAWQLLQEIKALFGNTEY 65

Query: 63  TVPFLLNLTASATFFAILSDAPISLAVPVTNATTFAATAVFGMLLGEDTRVGG 116
            +PFLLN + S  ++  L+   ++LAVP+ N+     T + G +LGED  +GG
Sbjct: 66  LMPFLLNQSGSLLYYLTLASTDLTLAVPICNSLAIVFTLIVGKVLGED--IGG 108

BLAST of Spg022725.1 vs. ExPASy TrEMBL
Match: A0A6J1HBE7 (transmembrane protein 234 homolog OS=Cucurbita moschata OX=3662 GN=LOC111461839 PE=3 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 4.3e-49
Identity = 107/114 (93.86%), Postives = 112/114 (98.25%), Query Frame = 0

Query: 1   MIGDVEKMVAIGLVWGATNALMRRGALLWDQALKSSSNSTHTSTKLLSSLRRWLKLLSIW 60
           MIGDVEKMVAIG+VWGATNALMRRGALLWDQALKSSSN+T +S KLLSSLRRWLKLLSIW
Sbjct: 1   MIGDVEKMVAIGIVWGATNALMRRGALLWDQALKSSSNATRSSNKLLSSLRRWLKLLSIW 60

Query: 61  QYTVPFLLNLTASATFFAILSDAPISLAVPVTNATTFAATAVFGMLLGEDTRVG 115
           QYTVPFL+NLTASATFFAILSDAPISLAVPVTNATTFAATAVFGMLLGE+TRVG
Sbjct: 61  QYTVPFLVNLTASATFFAILSDAPISLAVPVTNATTFAATAVFGMLLGEETRVG 114

BLAST of Spg022725.1 vs. ExPASy TrEMBL
Match: A0A6J1JK46 (transmembrane protein 234 homolog OS=Cucurbita maxima OX=3661 GN=LOC111485302 PE=3 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 3.7e-48
Identity = 105/114 (92.11%), Postives = 110/114 (96.49%), Query Frame = 0

Query: 1   MIGDVEKMVAIGLVWGATNALMRRGALLWDQALKSSSNSTHTSTKLLSSLRRWLKLLSIW 60
           MIGDVEKMVAIG+ WGATNALMRRGALLWDQALKSSSN+T +S KLLSS RRWLKLLSIW
Sbjct: 1   MIGDVEKMVAIGIAWGATNALMRRGALLWDQALKSSSNATRSSNKLLSSFRRWLKLLSIW 60

Query: 61  QYTVPFLLNLTASATFFAILSDAPISLAVPVTNATTFAATAVFGMLLGEDTRVG 115
           QYTVPFL+NLTASATFFAILSDAPISLAVPVTNATTFAATAVFGMLLGE+TRVG
Sbjct: 61  QYTVPFLVNLTASATFFAILSDAPISLAVPVTNATTFAATAVFGMLLGEETRVG 114

BLAST of Spg022725.1 vs. ExPASy TrEMBL
Match: A0A5A7VAD4 (Transmembrane protein 234-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold369G00120 PE=3 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 1.1e-44
Identity = 103/114 (90.35%), Postives = 107/114 (93.86%), Query Frame = 0

Query: 1   MIGDVEKMVAIGLVWGATNALMRRGALLWDQALKSSSNSTHTSTKLLSSLRRWLKLLSIW 60
           MIGDVEKMVAIGLVWGATNALMRRGALLWDQAL+SSS+    STK  SSLRRWLKL SIW
Sbjct: 1   MIGDVEKMVAIGLVWGATNALMRRGALLWDQALQSSSD----STKPFSSLRRWLKLFSIW 60

Query: 61  QYTVPFLLNLTASATFFAILSDAPISLAVPVTNATTFAATAVFGMLLGEDTRVG 115
           QYT+PFLLNLTASATFFAILSDAPISLAVPVTNATTFAATAVFG+LLGEDTRVG
Sbjct: 61  QYTLPFLLNLTASATFFAILSDAPISLAVPVTNATTFAATAVFGVLLGEDTRVG 110

BLAST of Spg022725.1 vs. ExPASy TrEMBL
Match: A0A1S4E4M5 (transmembrane protein 234 homolog OS=Cucumis melo OX=3656 GN=LOC103501037 PE=3 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 1.1e-44
Identity = 103/114 (90.35%), Postives = 107/114 (93.86%), Query Frame = 0

Query: 1   MIGDVEKMVAIGLVWGATNALMRRGALLWDQALKSSSNSTHTSTKLLSSLRRWLKLLSIW 60
           MIGDVEKMVAIGLVWGATNALMRRGALLWDQAL+SSS+    STK  SSLRRWLKL SIW
Sbjct: 11  MIGDVEKMVAIGLVWGATNALMRRGALLWDQALQSSSD----STKPFSSLRRWLKLFSIW 70

Query: 61  QYTVPFLLNLTASATFFAILSDAPISLAVPVTNATTFAATAVFGMLLGEDTRVG 115
           QYT+PFLLNLTASATFFAILSDAPISLAVPVTNATTFAATAVFG+LLGEDTRVG
Sbjct: 71  QYTLPFLLNLTASATFFAILSDAPISLAVPVTNATTFAATAVFGVLLGEDTRVG 120

BLAST of Spg022725.1 vs. ExPASy TrEMBL
Match: A0A6J1CV55 (transmembrane protein 234 homolog isoform X3 OS=Momordica charantia OX=3673 GN=LOC111014550 PE=3 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 7.9e-43
Identity = 99/114 (86.84%), Postives = 103/114 (90.35%), Query Frame = 0

Query: 1   MIGDVEKMVAIGLVWGATNALMRRGALLWDQALKSSSNSTHTSTKLLSSLRRWLKLLSIW 60
           MIGDVEKMVA+GLVWGATNALMRRGALLWDQAL SSSN +       SSL RWLKLLSIW
Sbjct: 1   MIGDVEKMVAVGLVWGATNALMRRGALLWDQALNSSSNGSS------SSLLRWLKLLSIW 60

Query: 61  QYTVPFLLNLTASATFFAILSDAPISLAVPVTNATTFAATAVFGMLLGEDTRVG 115
           QYT+PFLLNL+ASATFFAILSDAPISLAVPVTNATTFAATAVFGMLLGE TRVG
Sbjct: 61  QYTIPFLLNLSASATFFAILSDAPISLAVPVTNATTFAATAVFGMLLGEQTRVG 108

BLAST of Spg022725.1 vs. TAIR 10
Match: AT5G19570.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Uncharacterised protein family UPF0546 (InterPro:IPR018908); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 150.2 bits (378), Expect = 2.4e-36
Identity = 75/116 (64.66%), Postives = 96/116 (82.76%), Query Frame = 0

Query: 4   DVEKMVAIGLVWGATNALMRRGALLWDQALKSSSNSTHT-----STKLLSSLRRWLKLLS 63
           D++KMVA+GLVWGATNAL+RRGAL WD+   SS+  +H+       K++ +LR W+ LL 
Sbjct: 4   DMDKMVAVGLVWGATNALIRRGALAWDKTKSSSTTESHSPPLQIRRKIMIALRDWINLLL 63

Query: 64  IWQYTVPFLLNLTASATFFAILSDAPISLAVPVTNATTFAATAVFGMLLGEDTRVG 115
            WQY++PFL+NL+ASATFFA+LS APISLAVPVTNATTFAATA FG+LLGE+T++G
Sbjct: 64  FWQYSIPFLINLSASATFFALLSHAPISLAVPVTNATTFAATAAFGILLGEETQIG 119