Spg022554.1 (mRNA) Sponge gourd (cylindrica) v1

Overview


Name	Spg022554.1
Type	mRNA
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	protein ASPARTIC PROTEASE IN GUARD CELL 1-like
Location	scaffold2: 3731372 .. 3732829 (-)
Sequence length	1458
RNA-Seq Expression	Spg022554.1
Synteny	Spg022554.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACAACAACTGCTTTTTTTCCATCTTCCTCTTCCTAACACTCCTCAATTCCTCTTTCTTTTCTTCCTCTCTCTCTCGCTTGCTCACAGAATCGCCTCATTCCACCACACTCCTCGATGTCTCTGCCTCTTCCAAGCGAGCCCAAAACGCCCTCTCCATAAACCCTGTCCAAATCCTTCACTCCCATTCATTTCCTATTCTCAATCCAAATTCCTCTCTCTCTCTGCCATTGCACCCCAGATTGGCCATTCATAAGCCTTCGCCTTCTTACAAGGACTACGACAGCCTAGTCCGAGCCCGACTCGCCCGCGATGCCGCCCGGGTTCGCTCCCTCAACCGAAATCTCCAACTCGCTTTGACTGGGGCTGCAGTGGTCCGACCCGATTCCATAACCGCCCCTGTTGTTTCTGGCCAGAGCCAGGGGAGTGGGGAGTATTTTGCACGGATTGGCGTCGGGCAGCCTGCCCAGTCGTTCTACTTCGTGCCCGACACTGGCAGCGATGTCACGTGGCTTCAGTGCCTGCCCTGCAGTGATGCGAACGCCTGTTACAAACAAACCGACCCGATATTCGACCCCAAATCGTCGTCGTCTTACAGTCCTCTCTCCTGCGATTCGCAGCAATGTCAATCACTCGACAGAGGCAGTTGTCAATCCGGCACGTGTAATTACCAAGTCTCGTACGGCGACGGCTCATTCACGACCGGCGAATTCGCCACCGAAACGCTGTCGTTCGCGAATTCGAAATCCGTCCCCAATCTCCCCATCGGCTGCGGCCACGACAACGAAGGCCTCTTCGTCGGAGCCGCCGGTTTGATTGGCCTCGGCGGTGGGGATCTCTCCCTCTCCTCCCAGCTCAAAGCGTCGTCGTTTTCATACTGCCTCGTCGACCGCGACTCGGACTCGTCCTCGACTCTCGAGTTCAACTCCGGGCGACCCAGTGACTCCCTCACCTCTCCGCTCCTCAAAAACGACCGATTCGGCACGTACCGGTACGTGGAGGTCACCGGAATGAGCGTCGGCGGGAAGCCGCTGCCGATTTCGTCAACGAGATTCGATATCGATGGGTCGGGACTCGGGGGAATAATCGTGGACTCGGGGACGTTTATAACTCGGCTACCGAGTGACGTGTACGAATCGCTGAGAGACGCGTTTGTGAATCTGACGGGGAACTTGACGATGACGGGAGGGGTGTCGCCGTTCGACACGTGTTACGATTTTTCCGGTCAGTCGAGCGTGCAAGTGCCGACGGTGGCGTTTGAGTTGTCGAAGGGGAGCTCGCTGCGGCTGCCGGCGAATAACTACTTGATACGGATGGACTCGGCTGGAACTTTTTGCTTGGCGTTTCTTGCAACGACGTCGTCGCTTTCCATAATTGGGAGCTTCCAACAGCAGGGAATACGTGTCAGCTATGACCTGGTCAACTCTCTCGTCGGATTCTCATCCAACAAGTGTTAA

mRNA sequence

ATGAACAACAACTGCTTTTTTTCCATCTTCCTCTTCCTAACACTCCTCAATTCCTCTTTCTTTTCTTCCTCTCTCTCTCGCTTGCTCACAGAATCGCCTCATTCCACCACACTCCTCGATGTCTCTGCCTCTTCCAAGCGAGCCCAAAACGCCCTCTCCATAAACCCTGTCCAAATCCTTCACTCCCATTCATTTCCTATTCTCAATCCAAATTCCTCTCTCTCTCTGCCATTGCACCCCAGATTGGCCATTCATAAGCCTTCGCCTTCTTACAAGGACTACGACAGCCTAGTCCGAGCCCGACTCGCCCGCGATGCCGCCCGGGTTCGCTCCCTCAACCGAAATCTCCAACTCGCTTTGACTGGGGCTGCAGTGGTCCGACCCGATTCCATAACCGCCCCTGTTGTTTCTGGCCAGAGCCAGGGGAGTGGGGAGTATTTTGCACGGATTGGCGTCGGGCAGCCTGCCCAGTCGTTCTACTTCGTGCCCGACACTGGCAGCGATGTCACGTGGCTTCAGTGCCTGCCCTGCAGTGATGCGAACGCCTGTTACAAACAAACCGACCCGATATTCGACCCCAAATCGTCGTCGTCTTACAGTCCTCTCTCCTGCGATTCGCAGCAATGTCAATCACTCGACAGAGGCAGTTGTCAATCCGGCACGTGTAATTACCAAGTCTCGTACGGCGACGGCTCATTCACGACCGGCGAATTCGCCACCGAAACGCTGTCGTTCGCGAATTCGAAATCCGTCCCCAATCTCCCCATCGGCTGCGGCCACGACAACGAAGGCCTCTTCGTCGGAGCCGCCGGTTTGATTGGCCTCGGCGGTGGGGATCTCTCCCTCTCCTCCCAGCTCAAAGCGTCGTCGTTTTCATACTGCCTCGTCGACCGCGACTCGGACTCGTCCTCGACTCTCGAGTTCAACTCCGGGCGACCCAGTGACTCCCTCACCTCTCCGCTCCTCAAAAACGACCGATTCGGCACGTACCGGTACGTGGAGGTCACCGGAATGAGCGTCGGCGGGAAGCCGCTGCCGATTTCGTCAACGAGATTCGATATCGATGGGTCGGGACTCGGGGGAATAATCGTGGACTCGGGGACGTTTATAACTCGGCTACCGAGTGACGTGTACGAATCGCTGAGAGACGCGTTTGTGAATCTGACGGGGAACTTGACGATGACGGGAGGGGTGTCGCCGTTCGACACGTGTTACGATTTTTCCGGTCAGTCGAGCGTGCAAGTGCCGACGGTGGCGTTTGAGTTGTCGAAGGGGAGCTCGCTGCGGCTGCCGGCGAATAACTACTTGATACGGATGGACTCGGCTGGAACTTTTTGCTTGGCGTTTCTTGCAACGACGTCGTCGCTTTCCATAATTGGGAGCTTCCAACAGCAGGGAATACGTGTCAGCTATGACCTGGTCAACTCTCTCGTCGGATTCTCATCCAACAAGTGTTAA

Coding sequence (CDS)

ATGAACAACAACTGCTTTTTTTCCATCTTCCTCTTCCTAACACTCCTCAATTCCTCTTTCTTTTCTTCCTCTCTCTCTCGCTTGCTCACAGAATCGCCTCATTCCACCACACTCCTCGATGTCTCTGCCTCTTCCAAGCGAGCCCAAAACGCCCTCTCCATAAACCCTGTCCAAATCCTTCACTCCCATTCATTTCCTATTCTCAATCCAAATTCCTCTCTCTCTCTGCCATTGCACCCCAGATTGGCCATTCATAAGCCTTCGCCTTCTTACAAGGACTACGACAGCCTAGTCCGAGCCCGACTCGCCCGCGATGCCGCCCGGGTTCGCTCCCTCAACCGAAATCTCCAACTCGCTTTGACTGGGGCTGCAGTGGTCCGACCCGATTCCATAACCGCCCCTGTTGTTTCTGGCCAGAGCCAGGGGAGTGGGGAGTATTTTGCACGGATTGGCGTCGGGCAGCCTGCCCAGTCGTTCTACTTCGTGCCCGACACTGGCAGCGATGTCACGTGGCTTCAGTGCCTGCCCTGCAGTGATGCGAACGCCTGTTACAAACAAACCGACCCGATATTCGACCCCAAATCGTCGTCGTCTTACAGTCCTCTCTCCTGCGATTCGCAGCAATGTCAATCACTCGACAGAGGCAGTTGTCAATCCGGCACGTGTAATTACCAAGTCTCGTACGGCGACGGCTCATTCACGACCGGCGAATTCGCCACCGAAACGCTGTCGTTCGCGAATTCGAAATCCGTCCCCAATCTCCCCATCGGCTGCGGCCACGACAACGAAGGCCTCTTCGTCGGAGCCGCCGGTTTGATTGGCCTCGGCGGTGGGGATCTCTCCCTCTCCTCCCAGCTCAAAGCGTCGTCGTTTTCATACTGCCTCGTCGACCGCGACTCGGACTCGTCCTCGACTCTCGAGTTCAACTCCGGGCGACCCAGTGACTCCCTCACCTCTCCGCTCCTCAAAAACGACCGATTCGGCACGTACCGGTACGTGGAGGTCACCGGAATGAGCGTCGGCGGGAAGCCGCTGCCGATTTCGTCAACGAGATTCGATATCGATGGGTCGGGACTCGGGGGAATAATCGTGGACTCGGGGACGTTTATAACTCGGCTACCGAGTGACGTGTACGAATCGCTGAGAGACGCGTTTGTGAATCTGACGGGGAACTTGACGATGACGGGAGGGGTGTCGCCGTTCGACACGTGTTACGATTTTTCCGGTCAGTCGAGCGTGCAAGTGCCGACGGTGGCGTTTGAGTTGTCGAAGGGGAGCTCGCTGCGGCTGCCGGCGAATAACTACTTGATACGGATGGACTCGGCTGGAACTTTTTGCTTGGCGTTTCTTGCAACGACGTCGTCGCTTTCCATAATTGGGAGCTTCCAACAGCAGGGAATACGTGTCAGCTATGACCTGGTCAACTCTCTCGTCGGATTCTCATCCAACAAGTGTTAA

Protein sequence

MNNNCFFSIFLFLTLLNSSFFSSSLSRLLTESPHSTTLLDVSASSKRAQNALSINPVQILHSHSFPILNPNSSLSLPLHPRLAIHKPSPSYKDYDSLVRARLARDAARVRSLNRNLQLALTGAAVVRPDSITAPVVSGQSQGSGEYFARIGVGQPAQSFYFVPDTGSDVTWLQCLPCSDANACYKQTDPIFDPKSSSSYSPLSCDSQQCQSLDRGSCQSGTCNYQVSYGDGSFTTGEFATETLSFANSKSVPNLPIGCGHDNEGLFVGAAGLIGLGGGDLSLSSQLKASSFSYCLVDRDSDSSSTLEFNSGRPSDSLTSPLLKNDRFGTYRYVEVTGMSVGGKPLPISSTRFDIDGSGLGGIIVDSGTFITRLPSDVYESLRDAFVNLTGNLTMTGGVSPFDTCYDFSGQSSVQVPTVAFELSKGSSLRLPANNYLIRMDSAGTFCLAFLATTSSLSIIGSFQQQGIRVSYDLVNSLVGFSSNKC

Homology

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BLAST of Spg022554.1 vs. NCBI nr
Match: XP_023531915.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 721.1 bits (1860), Expect = 6.5e-204
Identity = 381/484 (78.72%), Postives = 418/484 (86.36%), Query Frame = 0


Query: 2   NNNCFFSIFLFLTLLNSSFFSSSLSRLLTESPHSTTLLDVSASSKRAQNALSINPVQILH 61
           NN   FS FL + +LNS  FSSSL+R+ T+   +T + DVSASS RAQNALSI P Q  H
Sbjct: 9   NNKPIFSTFLRIAILNSLLFSSSLARVFTQ---TTAVFDVSASSNRAQNALSITPPQ-FH 68

Query: 62  SHSFPILNPNSSLSLPLHPRLAIHKPSPSYKDYDSLVRARLARDAARVRSLNRNLQLALT 121
           SH       NSSLSLPLH RLAIHK   +YKDYDSLVRARLARDAARV+SLNRNL LAL 
Sbjct: 69  SHRL----SNSSLSLPLHSRLAIHK--HNYKDYDSLVRARLARDAARVQSLNRNLNLALA 128

Query: 122 GAAVVRPDSITAPVVSGQSQGSGEYFARIGVGQPAQSFYFVPDTGSDVTWLQCLPCSDAN 181
             A VRP+S+TAPVVSGQSQGSGEYFARI VGQPAQSFY VPDTGSD+TWLQCLPCS  N
Sbjct: 129 RDA-VRPNSLTAPVVSGQSQGSGEYFARIAVGQPAQSFYLVPDTGSDITWLQCLPCSYGN 188

Query: 182 ACYKQTDPIFDPKSSSSYSPLSCDSQQCQSLDRGSCQSGTCNYQVSYGDGSFTTGEFATE 241
            CY+QTDPIF+P SSSSY PLSCDSQQCQSLDR  CQSGTC YQV YGDGSFTTG+FATE
Sbjct: 189 TCYQQTDPIFNPTSSSSYRPLSCDSQQCQSLDRPGCQSGTCAYQVRYGDGSFTTGDFATE 248

Query: 242 TLSFANSKSVPNLPIGCGHDNEGLFVGAAGLIGLGGGDLSLSSQLKASSFSYCLVDRDSD 301
           TL+F NSKS+PNLPIGCGHDNEGLFVGAAGLIGLGGG LSLSSQLKASSFSYCLVDRDSD
Sbjct: 249 TLTFGNSKSIPNLPIGCGHDNEGLFVGAAGLIGLGGGALSLSSQLKASSFSYCLVDRDSD 308

Query: 302 SSSTLEFNSGRPSDSLTSPLLKNDRFGTYRYVEVTGMSVGGKPLPISSTRFDIDGSGLGG 361
           SSSTLEF+S RPSDS+T+PLLKN+R  +YRYV+VTGMSVGGK L ISSTRF+IDGSG+GG
Sbjct: 309 SSSTLEFDSARPSDSITTPLLKNNRIDSYRYVQVTGMSVGGKALSISSTRFEIDGSGMGG 368

Query: 362 IIVDSGTFITRLPSDVYESLRDAFVNLTGNLTMTGGVSPFDTCYDFSGQSSVQVPTVAFE 421
           IIVDSGTFITRLP+DVYESLR+AFV    +LT    +SPFDTCY+ +GQ++VQVPTVAFE
Sbjct: 369 IIVDSGTFITRLPTDVYESLREAFVQGAQSLTAASAISPFDTCYNLAGQANVQVPTVAFE 428

Query: 422 LSKGSSLRLPANNYLIRMDSAGTFCLAFLATTSSLSIIGSFQQQGIRVSYDLVNSLVGFS 481
           LSKG  L+LPA NYLIRMD+AGT+CLAFL TTSSLSIIGSFQQQG+RVSYDLVNSLVGFS
Sbjct: 429 LSKGKLLQLPARNYLIRMDTAGTYCLAFLKTTSSLSIIGSFQQQGMRVSYDLVNSLVGFS 481

Query: 482 SNKC 486
           SNKC
Sbjct: 489 SNKC 481

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BLAST of Spg022554.1 vs. NCBI nr
Match: KAG7021604.1 (Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 719.2 bits (1855), Expect = 2.5e-203
Identity = 380/484 (78.51%), Postives = 416/484 (85.95%), Query Frame = 0


Query: 2   NNNCFFSIFLFLTLLNSSFFSSSLSRLLTESPHSTTLLDVSASSKRAQNALSINPVQILH 61
           NN   FS  LF+ +LNS  FSSSL+R+  E   +TT+ DVSASS RAQNALSI P Q  H
Sbjct: 9   NNKPLFSTLLFIAILNSLLFSSSLARVFKE---TTTVFDVSASSNRAQNALSITPPQ-FH 68

Query: 62  SHSFPILNPNSSLSLPLHPRLAIHKPSPSYKDYDSLVRARLARDAARVRSLNRNLQLALT 121
           SH       NSSLSLPLH RLAIHK   SYKDY SLVRARLARDAARV+SLNRNL LAL 
Sbjct: 69  SHRL----SNSSLSLPLHSRLAIHK--HSYKDYQSLVRARLARDAARVQSLNRNLNLALA 128

Query: 122 GAAVVRPDSITAPVVSGQSQGSGEYFARIGVGQPAQSFYFVPDTGSDVTWLQCLPCSDAN 181
           G A VRP+S+TAPVVSGQSQGSGEYFARI VGQP QSFY VPDTGSD+TWLQCLPCS  N
Sbjct: 129 GDA-VRPNSLTAPVVSGQSQGSGEYFARIAVGQPPQSFYLVPDTGSDITWLQCLPCSYGN 188

Query: 182 ACYKQTDPIFDPKSSSSYSPLSCDSQQCQSLDRGSCQSGTCNYQVSYGDGSFTTGEFATE 241
            CY+QTDPIF+P SSSSY PLSCDSQQCQSL+R  CQSGTC YQV YGDGSFT G+F TE
Sbjct: 189 TCYQQTDPIFNPTSSSSYKPLSCDSQQCQSLNRPGCQSGTCVYQVRYGDGSFTVGDFVTE 248

Query: 242 TLSFANSKSVPNLPIGCGHDNEGLFVGAAGLIGLGGGDLSLSSQLKASSFSYCLVDRDSD 301
           TL+F NSKS+PNLPIGCGHDNEGLFVGAAGLIGLGGG LSLSSQLKASSFSYCLVDRDSD
Sbjct: 249 TLTFGNSKSIPNLPIGCGHDNEGLFVGAAGLIGLGGGALSLSSQLKASSFSYCLVDRDSD 308

Query: 302 SSSTLEFNSGRPSDSLTSPLLKNDRFGTYRYVEVTGMSVGGKPLPISSTRFDIDGSGLGG 361
           SSSTLEF+S  PSDS+T+PLLKN+R  +YRYV+VTGMSVGGK L ISSTRF+IDGSG+GG
Sbjct: 309 SSSTLEFDSAPPSDSITTPLLKNNRIDSYRYVQVTGMSVGGKALSISSTRFEIDGSGMGG 368

Query: 362 IIVDSGTFITRLPSDVYESLRDAFVNLTGNLTMTGGVSPFDTCYDFSGQSSVQVPTVAFE 421
           IIVDSGTFITRLP+DVYESLR+AFV    +LT  G +SPFDTCY+ +GQS+VQVPTVAFE
Sbjct: 369 IIVDSGTFITRLPTDVYESLREAFVQGAQSLTAAGAISPFDTCYNLAGQSNVQVPTVAFE 428

Query: 422 LSKGSSLRLPANNYLIRMDSAGTFCLAFLATTSSLSIIGSFQQQGIRVSYDLVNSLVGFS 481
           LSKG  L+LPANNYLIRMD+AG++CLAFL TTSSLSIIGSFQQQG+RVSYDLVNSLVGFS
Sbjct: 429 LSKGKLLQLPANNYLIRMDTAGSYCLAFLKTTSSLSIIGSFQQQGMRVSYDLVNSLVGFS 481

Query: 482 SNKC 486
           SNKC
Sbjct: 489 SNKC 481

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BLAST of Spg022554.1 vs. NCBI nr
Match: XP_023007215.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita maxima])

HSP 1 Score: 718.8 bits (1854), Expect = 3.2e-203
Identity = 383/485 (78.97%), Postives = 420/485 (86.60%), Query Frame = 0


Query: 2   NNNCFFSIFLFLTLLNSSFFSSSLSRLLTESPHSTTLLDVSASSKRAQNALSINPVQILH 61
           NN   FS FLF  +LNS  FSSSL+R+ TE   +TT+ DVSASS RAQNALSI P Q  H
Sbjct: 9   NNKPIFSAFLFTAILNSLLFSSSLARVFTE---TTTVFDVSASSNRAQNALSITPPQ-FH 68

Query: 62  SHSFPILNPNSSLSLPLHPRLAIHKPSPSYKDYDSLVRARLARDAARVRSLNRNLQLALT 121
           SH       NSSLSL LH RLAIHK   +YKDY+SLVRARLARDAARV+SLNRNL LAL 
Sbjct: 69  SHHL----SNSSLSLSLHSRLAIHK--HNYKDYESLVRARLARDAARVQSLNRNLNLALA 128

Query: 122 GAAVVRPDSITAPVVSGQSQGSGEYFARIGVGQPAQSFYFVPDTGSDVTWLQCLPCSDAN 181
           G A VRP+S+TAPVVSGQSQGSGEYFARI VGQPAQSFY VPDTGSD+TWLQCLPCS  N
Sbjct: 129 GDA-VRPNSLTAPVVSGQSQGSGEYFARIAVGQPAQSFYLVPDTGSDITWLQCLPCSIGN 188

Query: 182 ACYKQTDPIFDPKSSSSYSPLSCDSQQCQSLDRGSCQSGTCNYQVSYGDGSFTTGEFATE 241
            CY QTDPIF+P SSSSY PLSCDSQQCQSL+R  CQSGTC YQV YGDGSFTTG+FATE
Sbjct: 189 TCYPQTDPIFNPTSSSSYRPLSCDSQQCQSLNRPGCQSGTCVYQVWYGDGSFTTGDFATE 248

Query: 242 TLSFANSKSVPNLPIGCGHDNEGLFVGAAGLIGLGGGDLSLSSQLKASSFSYCLVDRDSD 301
           TL+F NSKS+PNLPIGCGHDN+GLFVGAAGLIGLGGG LSLSSQLKASSFSYCLVDRDSD
Sbjct: 249 TLTFGNSKSIPNLPIGCGHDNKGLFVGAAGLIGLGGGALSLSSQLKASSFSYCLVDRDSD 308

Query: 302 SSSTLEFNSGRPSDSLTSPLLKNDRFGTYRYVEVTGMSVGGKPLPISSTRFDIDGSGLGG 361
           SSSTLEF+S RPSDS+T+PLLKN+R  +YRYV+VTGMSVGGK L ISSTRF+IDGSG+GG
Sbjct: 309 SSSTLEFDSARPSDSITTPLLKNNRIDSYRYVQVTGMSVGGKALSISSTRFEIDGSGMGG 368

Query: 362 IIVDSGTFITRLPSDVYESLRDAFVNLTGNLTMTGGVSPFDTCYDFSGQSSVQVPTVAFE 421
           IIVDSGTFITRLP+DVYESLR+AFV    +LT  G +SPFDTCY+ +GQS+VQVPTVAFE
Sbjct: 369 IIVDSGTFITRLPTDVYESLREAFVQGARSLTAAGAISPFDTCYNLAGQSNVQVPTVAFE 428

Query: 422 LSKGSSLRLPANNYLIRMDSAGTFCLAFL-ATTSSLSIIGSFQQQGIRVSYDLVNSLVGF 481
           LSKG+ L+LPA NYLIRMD+AGT+CLAFL  TTSSLSIIGSFQQQG+RVSYDLVNSLVGF
Sbjct: 429 LSKGNLLQLPARNYLIRMDTAGTYCLAFLKLTTSSLSIIGSFQQQGMRVSYDLVNSLVGF 482

Query: 482 SSNKC 486
           SSNKC
Sbjct: 489 SSNKC 482

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BLAST of Spg022554.1 vs. NCBI nr
Match: XP_022933467.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita moschata] >KAG6587644.1 Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 716.5 bits (1848), Expect = 1.6e-202
Identity = 378/484 (78.10%), Postives = 417/484 (86.16%), Query Frame = 0


Query: 2   NNNCFFSIFLFLTLLNSSFFSSSLSRLLTESPHSTTLLDVSASSKRAQNALSINPVQILH 61
           NN   FS  LF+ +LNS  FSSSL+R+  E   +TT+ DVSASS RAQ+ALS+ P Q  H
Sbjct: 9   NNKPLFSTLLFIAILNSLLFSSSLARVFKE---TTTVFDVSASSNRAQSALSMTPPQ-FH 68

Query: 62  SHSFPILNPNSSLSLPLHPRLAIHKPSPSYKDYDSLVRARLARDAARVRSLNRNLQLALT 121
           SH       NSSLSLPLH RLAIHK   SYKDY+SLVRARLARDAARV+SLNRNL LAL 
Sbjct: 69  SHRL----SNSSLSLPLHSRLAIHK--HSYKDYESLVRARLARDAARVQSLNRNLNLALA 128

Query: 122 GAAVVRPDSITAPVVSGQSQGSGEYFARIGVGQPAQSFYFVPDTGSDVTWLQCLPCSDAN 181
           G A VRP+S+TAPVVSGQSQGSGEYFARI VGQP QSFY VPDTGSD+TWLQCLPCS  N
Sbjct: 129 GDA-VRPNSLTAPVVSGQSQGSGEYFARIAVGQPPQSFYLVPDTGSDITWLQCLPCSYGN 188

Query: 182 ACYKQTDPIFDPKSSSSYSPLSCDSQQCQSLDRGSCQSGTCNYQVSYGDGSFTTGEFATE 241
            CY+QTDPIF+P SSSSY PLSCDSQQCQSL+R  CQSGTC YQV YGDGSFT G+F TE
Sbjct: 189 TCYQQTDPIFNPTSSSSYRPLSCDSQQCQSLNRPGCQSGTCVYQVRYGDGSFTVGDFVTE 248

Query: 242 TLSFANSKSVPNLPIGCGHDNEGLFVGAAGLIGLGGGDLSLSSQLKASSFSYCLVDRDSD 301
           TL+F NSKS+PNLPIGCGHDNEGLFVGAAGLIGLGGG LSLSSQLKASSFSYCLVDRDSD
Sbjct: 249 TLTFGNSKSIPNLPIGCGHDNEGLFVGAAGLIGLGGGALSLSSQLKASSFSYCLVDRDSD 308

Query: 302 SSSTLEFNSGRPSDSLTSPLLKNDRFGTYRYVEVTGMSVGGKPLPISSTRFDIDGSGLGG 361
           SSSTLEF+S  PSDS+T+PLLKN+R  +YRYV+VTGMSVGGK L ISSTRF+IDGSG+GG
Sbjct: 309 SSSTLEFDSAPPSDSITTPLLKNNRIDSYRYVQVTGMSVGGKALSISSTRFEIDGSGMGG 368

Query: 362 IIVDSGTFITRLPSDVYESLRDAFVNLTGNLTMTGGVSPFDTCYDFSGQSSVQVPTVAFE 421
           IIVDSGTFITRLP+DVYESLR+AFV    +LT  G +SPFDTCY+ +GQS+VQVPTVAFE
Sbjct: 369 IIVDSGTFITRLPTDVYESLREAFVQGAQSLTAAGAISPFDTCYNLAGQSNVQVPTVAFE 428

Query: 422 LSKGSSLRLPANNYLIRMDSAGTFCLAFLATTSSLSIIGSFQQQGIRVSYDLVNSLVGFS 481
           LSKG  L+LPANNYLIRMD+AG++CLAFL TTSSLSIIGSFQQQG+RVSYDLVNSLVGFS
Sbjct: 429 LSKGKLLQLPANNYLIRMDTAGSYCLAFLKTTSSLSIIGSFQQQGMRVSYDLVNSLVGFS 481

Query: 482 SNKC 486
           SNKC
Sbjct: 489 SNKC 481

[Close]

BLAST of Spg022554.1 vs. NCBI nr
Match: XP_038878113.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1 [Benincasa hispida])

HSP 1 Score: 677.9 bits (1748), Expect = 6.3e-191
Identity = 361/488 (73.98%), Postives = 399/488 (81.76%), Query Frame = 0


Query: 8   SIFLFLTLLNSSFFSSSLSRLLTESPHSTTLLDVSASSKRAQNALSINPVQI-LHSHSFP 67
           S F+FLT+L S  F S  SR LT+SP+ST + DVSAS+K+AQNALSI P     HSH   
Sbjct: 7   SAFIFLTILTSLQFPSIFSRKLTQSPYSTAIFDVSASTKQAQNALSIKPKPFETHSH--- 66

Query: 68  ILNPNSSLSLPLHPRLAIHKPSPSYKDYDSLVRARLARDAARVRSLNRNLQLALTGAAVV 127
             +PNS LSLPLHPRL ++  +PSYKDY SLVRARLARDA RV+SLNRNL+L+L G   +
Sbjct: 67  --HPNSPLSLPLHPRLTLY--NPSYKDYGSLVRARLARDAVRVQSLNRNLELSLNGGQKI 126

Query: 128 --------RPDSITAPVVSGQSQGS-GEYFARIGVGQPAQSFYFVPDTGSDVTWLQCLPC 187
                     DSITAPVVSGQS G+ GEYFARIGVGQP QSFY VPDTGSDVTWLQC PC
Sbjct: 127 GAGINGSESADSITAPVVSGQSLGNGGEYFARIGVGQPVQSFYLVPDTGSDVTWLQCQPC 186

Query: 188 SDANACYKQTDPIFDPKSSSSYSPLSCDSQQCQSLDRGSCQSGTCNYQVSYGDGSFTTGE 247
           +   ACYKQ DPIFDPKSSSSY+ LSC+SQQCQ LD+ +C S  C YQV+YGDGSFTTGE
Sbjct: 187 AAEKACYKQIDPIFDPKSSSSYTALSCNSQQCQLLDKANCNSDVCQYQVNYGDGSFTTGE 246

Query: 248 FATETLSFANSKSVPNLPIGCGHDNEGLFVGAAGLIGLGGGDLSLSSQLKASSFSYCLVD 307
            ATETLSF NS S+PNLPIGCGHDNEGLFVGAAGLIGLGGG +SLSSQLKASSFSYCLVD
Sbjct: 247 LATETLSFGNSNSIPNLPIGCGHDNEGLFVGAAGLIGLGGGAISLSSQLKASSFSYCLVD 306

Query: 308 RDSDSSSTLEFNSGRPSDSLTSPLLKNDRFGTYRYVEVTGMSVGGKPLPISSTRFDIDGS 367
            DSDSSSTLEFN+ RPSDSLTSPL+KNDRF +YRYV+V GMSVGG PLPISSTRF+ID S
Sbjct: 307 LDSDSSSTLEFNTDRPSDSLTSPLVKNDRFHSYRYVKVVGMSVGGNPLPISSTRFEIDES 366

Query: 368 GLGGIIVDSGTFITRLPSDVYESLRDAFVNLTGNLTMTGGVSPFDTCYDFSGQSSVQVPT 427
           GLGGIIVDSGT IT+LPSDVYESLR+AFV  T NL    G+S FDTCYD SGQSSV+VP 
Sbjct: 367 GLGGIIVDSGTTITQLPSDVYESLREAFVKQTSNLPPAQGISLFDTCYDLSGQSSVEVPV 426

Query: 428 VAFELSKGSSLRLPANNYLIRMDSAGTFCLAFLATTSSLSIIGSFQQQGIRVSYDLVNSL 486
           +AF L   +SLRLPA NYLI +DS GT+CLAF  T SSLSIIGSFQQQGIRVSYDL NSL
Sbjct: 427 IAFVLPGENSLRLPAKNYLIPVDSDGTYCLAFFKTRSSLSIIGSFQQQGIRVSYDLANSL 486

[Close]

BLAST of Spg022554.1 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 1.5e-126
Identity = 251/499 (50.30%), Postives = 338/499 (67.74%), Query Frame = 0


Query: 8   SIFLFLTLLNSSFFSSSLSRLLTESPHSTTLLDVSASSKRAQNALSINPVQILHSHSFP- 67
           ++ LFLT  ++S  S SLS     +P  T +LDV +S ++ Q  LS++P +   + + P 
Sbjct: 14  TLSLFLTTTDAS--SRSLS-----TPPKTNVLDVVSSLQQTQTILSLDPTRSSLTTTKPE 73

Query: 68  ------ILNPNSSLSLPLHPRLAIHKPSPSYKDYDSLVRARLARDAARVRSLNRNLQLAL 127
                   N +S LSL LH R      +  +KDY SL  +RL RD++RV  +   ++ A+
Sbjct: 74  SLSDPVFFNSSSPLSLELHSRDTF--VASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAV 133

Query: 128 TGA--AVVRP----------DSITAPVVSGQSQGSGEYFARIGVGQPAQSFYFVPDTGSD 187
            G   + ++P          + +T PVVSG SQGSGEYF+RIGVG PA+  Y V DTGSD
Sbjct: 134 EGVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSD 193

Query: 188 VTWLQCLPCSDANACYKQTDPIFDPKSSSSYSPLSCDSQQCQSLDRGSCQSGTCNYQVSY 247
           V W+QC PC+D   CY+Q+DP+F+P SSS+Y  L+C + QC  L+  +C+S  C YQVSY
Sbjct: 194 VNWIQCEPCAD---CYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSY 253

Query: 248 GDGSFTTGEFATETLSFANSKSVPNLPIGCGHDNEGLFVGAAGLIGLGGGDLSLSSQLKA 307
           GDGSFT GE AT+T++F NS  + N+ +GCGHDNEGLF GAAGL+GLGGG LS+++Q+KA
Sbjct: 254 GDGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKA 313

Query: 308 SSFSYCLVDRDSDSSSTLEFNSGR-PSDSLTSPLLKNDRFGTYRYVEVTGMSVGGKPLPI 367
           +SFSYCLVDRDS  SS+L+FNS +      T+PLL+N +  T+ YV ++G SVGG+ + +
Sbjct: 314 TSFSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVL 373

Query: 368 SSTRFDIDGSGLGGIIVDSGTFITRLPSDVYESLRDAFVNLTGNLTM-TGGVSPFDTCYD 427
               FD+D SG GG+I+D GT +TRL +  Y SLRDAF+ LT NL   +  +S FDTCYD
Sbjct: 374 PDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYD 433

Query: 428 FSGQSSVQVPTVAFELSKGSSLRLPANNYLIRMDSAGTFCLAFLATTSSLSIIGSFQQQG 486
           FS  S+V+VPTVAF  + G SL LPA NYLI +D +GTFC AF  T+SSLSIIG+ QQQG
Sbjct: 434 FSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQG 493

[Close]

BLAST of Spg022554.1 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 3.8e-98
Identity = 187/409 (45.72%), Postives = 259/409 (63.33%), Query Frame = 0


Query: 87  PSPSYKDYDSLVRARLARDAARVRSLNRNLQLALTGAAVVRPDS------ITAPVVSGQS 146
           PS +Y+++   + AR+ RD  RV ++ R     ++G  +   DS        + +VSG  
Sbjct: 70  PSVTYRNHHHRLHARMRRDTDRVSAILRR----ISGKVIPSSDSRYEVNDFGSDIVSGMD 129

Query: 147 QGSGEYFARIGVGQPAQSFYFVPDTGSDVTWLQCLPCSDANACYKQTDPIFDPKSSSSYS 206
           QGSGEYF RIGVG P +  Y V D+GSD+ W+QC PC     CYKQ+DP+FDP  S SY+
Sbjct: 130 QGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPC---KLCYKQSDPVFDPAKSGSYT 189

Query: 207 PLSCDSQQCQSLDRGSCQSGTCNYQVSYGDGSFTTGEFATETLSFANSKSVPNLPIGCGH 266
            +SC S  C  ++   C SG C Y+V YGDGS+T G  A ETL+FA +  V N+ +GCGH
Sbjct: 190 GVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTFAKT-VVRNVAMGCGH 249

Query: 267 DNEGLFVGAAGLIGLGGGDLSLSSQLK---ASSFSYCLVDRDSDSSSTLEF-NSGRPSDS 326
            N G+F+GAAGL+G+GGG +S   QL      +F YCLV R +DS+ +L F     P  +
Sbjct: 250 RNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDSTGSLVFGREALPVGA 309

Query: 327 LTSPLLKNDRFGTYRYVEVTGMSVGGKPLPISSTRFDIDGSGLGGIIVDSGTFITRLPSD 386
              PL++N R  ++ YV + G+ VGG  +P+    FD+  +G GG+++D+GT +TRLP+ 
Sbjct: 310 SWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMDTGTAVTRLPTA 369

Query: 387 VYESLRDAFVNLTGNLTMTGGVSPFDTCYDFSGQSSVQVPTVAFELSKGSSLRLPANNYL 446
            Y + RD F + T NL    GVS FDTCYD SG  SV+VPTV+F  ++G  L LPA N+L
Sbjct: 370 AYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPARNFL 429

Query: 447 IRMDSAGTFCLAFLATTSSLSIIGSFQQQGIRVSYDLVNSLVGFSSNKC 486
           + +D +GT+C AF A+ + LSIIG+ QQ+GI+VS+D  N  VGF  N C
Sbjct: 430 MPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

[Close]

BLAST of Spg022554.1 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 1.5e-94
Identity = 198/407 (48.65%), Postives = 254/407 (62.41%), Query Frame = 0


Query: 90  SYKDYDSLVRARLARDAARVRSLNRNLQLALTGAAVV---RPDSITAPVVSGQSQGSGEY 149
           S K  D L  +RL RD+ RV+S+   L   + G  V    RP   ++ VVSG SQGSGEY
Sbjct: 84  SNKTPDELFSSRLQRDSRRVKSI-ATLAAQIPGRNVTHAPRPGGFSSSVVSGLSQGSGEY 143

Query: 150 FARIGVGQPAQSFYFVPDTGSDVTWLQCLPCSDANACYKQTDPIFDPKSSSSYSPLSCDS 209
           F R+GVG PA+  Y V DTGSD+ WLQC PC     CY Q+DPIFDP+ S +Y+ + C S
Sbjct: 144 FTRLGVGTPARYVYMVLDTGSDIVWLQCAPC---RRCYSQSDPIFDPRKSKTYATIPCSS 203

Query: 210 QQCQSLDRGSCQS--GTCNYQVSYGDGSFTTGEFATETLSFANSKSVPNLPIGCGHDNEG 269
             C+ LD   C +   TC YQVSYGDGSFT G+F+TETL+F  ++ V  + +GCGHDNEG
Sbjct: 204 PHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNR-VKGVALGCGHDNEG 263

Query: 270 LFVGAAGLIGLGGGDLSLSSQLK---ASSFSYCLVDRDSDS--SSTLEFNSGRPSDSLTS 329
           LFVGAAGL+GLG G LS   Q        FSYCLVDR + S  SS +  N+     +  +
Sbjct: 264 LFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNAAVSRIARFT 323

Query: 330 PLLKNDRFGTYRYVEVTGMSVGGKPLP-ISSTRFDIDGSGLGGIIVDSGTFITRLPSDVY 389
           PLL N +  T+ YV + G+SVGG  +P ++++ F +D  G GG+I+DSGT +TRL    Y
Sbjct: 324 PLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAY 383

Query: 390 ESLRDAFVNLTGNLTMTGGVSPFDTCYDFSGQSSVQVPTVAFELSKGSSLRLPANNYLIR 449
            ++RDAF      L      S FDTC+D S  + V+VPTV     +G+ + LPA NYLI 
Sbjct: 384 IAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHF-RGADVSLPATNYLIP 443

Query: 450 MDSAGTFCLAFLATTSSLSIIGSFQQQGIRVSYDLVNSLVGFSSNKC 486
           +D+ G FC AF  T   LSIIG+ QQQG RV YDL +S VGF+   C
Sbjct: 444 VDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGC 484

[Close]

BLAST of Spg022554.1 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 7.0e-68
Identity = 146/350 (41.71%), Postives = 205/350 (58.57%), Query Frame = 0


Query: 142 GSGEYFARIGVGQPAQSFYFVPDTGSDVTWLQCLPCSDANACYKQTDPIFDPKSSSSYSP 201
           G GEY   + +G PAQ F  + DTGSD+ W QC PC+    C+ Q+ PIF+P+ SSS+S 
Sbjct: 91  GDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQ---CFNQSTPIFNPQGSSSFST 150

Query: 202 LSCDSQQCQSLDRGSCQSGTCNYQVSYGDGSFTTGEFATETLSFANSKSVPNLPIGCGHD 261
           L C SQ CQ+L   +C +  C Y   YGDGS T G   TETL+F  S S+PN+  GCG +
Sbjct: 151 LPCSSQLCQALSSPTCSNNFCQYTYGYGDGSETQGSMGTETLTF-GSVSIPNITFGCGEN 210

Query: 262 NEGLFVG-AAGLIGLGGGDLSLSSQLKASSFSYCLVDRDSDSSSTLEFNSGRPSDSLTSP 321
           N+G   G  AGL+G+G G LSL SQL  + FSYC+    S + S L   S   S +  SP
Sbjct: 211 NQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGSP 270

Query: 322 ---LLKNDRFGTYRYVEVTGMSVGGKPLPISSTRFDID-GSGLGGIIVDSGTFITRLPSD 381
              L+++ +  T+ Y+ + G+SVG   LPI  + F ++  +G GGII+DSGT +T   ++
Sbjct: 271 NTTLIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNN 330

Query: 382 VYESLRDAFVNLTGNLTMTGGVSPFDTCYDF-SGQSSVQVPTVAFELSKGSSLRLPANNY 441
            Y+S+R  F++      + G  S FD C+   S  S++Q+PT       G  L LP+ NY
Sbjct: 331 AYQSVRQEFISQINLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFD-GGDLELPSENY 390

Query: 442 LIRMDSAGTFCLAFLATTSSLSIIGSFQQQGIRVSYDLVNSLVGFSSNKC 486
            I   S G  CLA  +++  +SI G+ QQQ + V YD  NS+V F+S +C
Sbjct: 391 FI-SPSNGLICLAMGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of Spg022554.1 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 4.5e-67
Identity = 160/395 (40.51%), Postives = 214/395 (54.18%), Query Frame = 0


Query: 97  LVRARLARDAARVRSLNRNLQLALTGAAVVRPDSITAPVVSGQSQGSGEYFARIGVGQPA 156
           L++  + R   R+RS+N  LQ +           I  PV +    G GEY   + +G P 
Sbjct: 60  LIKRAIKRGERRMRSINAMLQSS---------SGIETPVYA----GDGEYLMNVAIGTPD 119

Query: 157 QSFYFVPDTGSDVTWLQCLPCSDANACYKQTDPIFDPKSSSSYSPLSCDSQQCQSLDRGS 216
            SF  + DTGSD+ W QC PC+    C+ Q  PIF+P+ SSS+S L C+SQ CQ L   +
Sbjct: 120 SSFSAIMDTGSDLIWTQCEPCTQ---CFSQPTPIFNPQDSSSFSTLPCESQYCQDLPSET 179

Query: 217 CQSGTCNYQVSYGDGSFTTGEFATETLSFANSKSVPNLPIGCGHDNEGLFVG-AAGLIGL 276
           C +  C Y   YGDGS T G  ATET +F  S SVPN+  GCG DN+G   G  AGLIG+
Sbjct: 180 CNNNECQYTYGYGDGSTTQGYMATETFTFETS-SVPNIAFGCGEDNQGFGQGNGAGLIGM 239

Query: 277 GGGDLSLSSQLKASSFSYCLVDRDSDSSSTLEFN---SGRPSDSLTSPLLKNDRFGTYRY 336
           G G LSL SQL    FSYC+    S S STL      SG P  S ++ L+ +    TY Y
Sbjct: 240 GWGPLSLPSQLGVGQFSYCMTSYGSSSPSTLALGSAASGVPEGSPSTTLIHSSLNPTYYY 299

Query: 337 VEVTGMSVGGKPLPISSTRFDIDGSGLGGIIVDSGTFITRLPSDVYESLRDAFVNLTGNL 396
           + + G++VGG  L I S+ F +   G GG+I+DSGT +T LP D Y ++  AF +     
Sbjct: 300 ITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLP 359

Query: 397 TMTGGVSPFDTCYDF-SGQSSVQVPTVAFELSKGSSLRLPANNYLIRMDSAGTFCLAFLA 456
           T+    S   TC+   S  S+VQVP ++ +   G  L L   N LI   + G  CLA  +
Sbjct: 360 TVDESSSGLSTCFQQPSDGSTVQVPEISMQFD-GGVLNLGEQNILI-SPAEGVICLAMGS 419

Query: 457 TTS-SLSIIGSFQQQGIRVSYDLVNSLVGFSSNKC 486
           ++   +SI G+ QQQ  +V YDL N  V F   +C
Sbjct: 420 SSQLGISIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of Spg022554.1 vs. ExPASy TrEMBL
Match: A0A6J1L2D2 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita maxima OX=3661 GN=LOC111499769 PE=4 SV=1)

HSP 1 Score: 718.8 bits (1854), Expect = 1.6e-203
Identity = 383/485 (78.97%), Postives = 420/485 (86.60%), Query Frame = 0


Query: 2   NNNCFFSIFLFLTLLNSSFFSSSLSRLLTESPHSTTLLDVSASSKRAQNALSINPVQILH 61
           NN   FS FLF  +LNS  FSSSL+R+ TE   +TT+ DVSASS RAQNALSI P Q  H
Sbjct: 9   NNKPIFSAFLFTAILNSLLFSSSLARVFTE---TTTVFDVSASSNRAQNALSITPPQ-FH 68

Query: 62  SHSFPILNPNSSLSLPLHPRLAIHKPSPSYKDYDSLVRARLARDAARVRSLNRNLQLALT 121
           SH       NSSLSL LH RLAIHK   +YKDY+SLVRARLARDAARV+SLNRNL LAL 
Sbjct: 69  SHHL----SNSSLSLSLHSRLAIHK--HNYKDYESLVRARLARDAARVQSLNRNLNLALA 128

Query: 122 GAAVVRPDSITAPVVSGQSQGSGEYFARIGVGQPAQSFYFVPDTGSDVTWLQCLPCSDAN 181
           G A VRP+S+TAPVVSGQSQGSGEYFARI VGQPAQSFY VPDTGSD+TWLQCLPCS  N
Sbjct: 129 GDA-VRPNSLTAPVVSGQSQGSGEYFARIAVGQPAQSFYLVPDTGSDITWLQCLPCSIGN 188

Query: 182 ACYKQTDPIFDPKSSSSYSPLSCDSQQCQSLDRGSCQSGTCNYQVSYGDGSFTTGEFATE 241
            CY QTDPIF+P SSSSY PLSCDSQQCQSL+R  CQSGTC YQV YGDGSFTTG+FATE
Sbjct: 189 TCYPQTDPIFNPTSSSSYRPLSCDSQQCQSLNRPGCQSGTCVYQVWYGDGSFTTGDFATE 248

Query: 242 TLSFANSKSVPNLPIGCGHDNEGLFVGAAGLIGLGGGDLSLSSQLKASSFSYCLVDRDSD 301
           TL+F NSKS+PNLPIGCGHDN+GLFVGAAGLIGLGGG LSLSSQLKASSFSYCLVDRDSD
Sbjct: 249 TLTFGNSKSIPNLPIGCGHDNKGLFVGAAGLIGLGGGALSLSSQLKASSFSYCLVDRDSD 308

Query: 302 SSSTLEFNSGRPSDSLTSPLLKNDRFGTYRYVEVTGMSVGGKPLPISSTRFDIDGSGLGG 361
           SSSTLEF+S RPSDS+T+PLLKN+R  +YRYV+VTGMSVGGK L ISSTRF+IDGSG+GG
Sbjct: 309 SSSTLEFDSARPSDSITTPLLKNNRIDSYRYVQVTGMSVGGKALSISSTRFEIDGSGMGG 368

Query: 362 IIVDSGTFITRLPSDVYESLRDAFVNLTGNLTMTGGVSPFDTCYDFSGQSSVQVPTVAFE 421
           IIVDSGTFITRLP+DVYESLR+AFV    +LT  G +SPFDTCY+ +GQS+VQVPTVAFE
Sbjct: 369 IIVDSGTFITRLPTDVYESLREAFVQGARSLTAAGAISPFDTCYNLAGQSNVQVPTVAFE 428

Query: 422 LSKGSSLRLPANNYLIRMDSAGTFCLAFL-ATTSSLSIIGSFQQQGIRVSYDLVNSLVGF 481
           LSKG+ L+LPA NYLIRMD+AGT+CLAFL  TTSSLSIIGSFQQQG+RVSYDLVNSLVGF
Sbjct: 429 LSKGNLLQLPARNYLIRMDTAGTYCLAFLKLTTSSLSIIGSFQQQGMRVSYDLVNSLVGF 482

Query: 482 SSNKC 486
           SSNKC
Sbjct: 489 SSNKC 482

[Close]

BLAST of Spg022554.1 vs. ExPASy TrEMBL
Match: A0A6J1EZU8 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=LOC111440870 PE=4 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 7.7e-203
Identity = 378/484 (78.10%), Postives = 417/484 (86.16%), Query Frame = 0


Query: 2   NNNCFFSIFLFLTLLNSSFFSSSLSRLLTESPHSTTLLDVSASSKRAQNALSINPVQILH 61
           NN   FS  LF+ +LNS  FSSSL+R+  E   +TT+ DVSASS RAQ+ALS+ P Q  H
Sbjct: 9   NNKPLFSTLLFIAILNSLLFSSSLARVFKE---TTTVFDVSASSNRAQSALSMTPPQ-FH 68

Query: 62  SHSFPILNPNSSLSLPLHPRLAIHKPSPSYKDYDSLVRARLARDAARVRSLNRNLQLALT 121
           SH       NSSLSLPLH RLAIHK   SYKDY+SLVRARLARDAARV+SLNRNL LAL 
Sbjct: 69  SHRL----SNSSLSLPLHSRLAIHK--HSYKDYESLVRARLARDAARVQSLNRNLNLALA 128

Query: 122 GAAVVRPDSITAPVVSGQSQGSGEYFARIGVGQPAQSFYFVPDTGSDVTWLQCLPCSDAN 181
           G A VRP+S+TAPVVSGQSQGSGEYFARI VGQP QSFY VPDTGSD+TWLQCLPCS  N
Sbjct: 129 GDA-VRPNSLTAPVVSGQSQGSGEYFARIAVGQPPQSFYLVPDTGSDITWLQCLPCSYGN 188

Query: 182 ACYKQTDPIFDPKSSSSYSPLSCDSQQCQSLDRGSCQSGTCNYQVSYGDGSFTTGEFATE 241
            CY+QTDPIF+P SSSSY PLSCDSQQCQSL+R  CQSGTC YQV YGDGSFT G+F TE
Sbjct: 189 TCYQQTDPIFNPTSSSSYRPLSCDSQQCQSLNRPGCQSGTCVYQVRYGDGSFTVGDFVTE 248

Query: 242 TLSFANSKSVPNLPIGCGHDNEGLFVGAAGLIGLGGGDLSLSSQLKASSFSYCLVDRDSD 301
           TL+F NSKS+PNLPIGCGHDNEGLFVGAAGLIGLGGG LSLSSQLKASSFSYCLVDRDSD
Sbjct: 249 TLTFGNSKSIPNLPIGCGHDNEGLFVGAAGLIGLGGGALSLSSQLKASSFSYCLVDRDSD 308

Query: 302 SSSTLEFNSGRPSDSLTSPLLKNDRFGTYRYVEVTGMSVGGKPLPISSTRFDIDGSGLGG 361
           SSSTLEF+S  PSDS+T+PLLKN+R  +YRYV+VTGMSVGGK L ISSTRF+IDGSG+GG
Sbjct: 309 SSSTLEFDSAPPSDSITTPLLKNNRIDSYRYVQVTGMSVGGKALSISSTRFEIDGSGMGG 368

Query: 362 IIVDSGTFITRLPSDVYESLRDAFVNLTGNLTMTGGVSPFDTCYDFSGQSSVQVPTVAFE 421
           IIVDSGTFITRLP+DVYESLR+AFV    +LT  G +SPFDTCY+ +GQS+VQVPTVAFE
Sbjct: 369 IIVDSGTFITRLPTDVYESLREAFVQGAQSLTAAGAISPFDTCYNLAGQSNVQVPTVAFE 428

Query: 422 LSKGSSLRLPANNYLIRMDSAGTFCLAFLATTSSLSIIGSFQQQGIRVSYDLVNSLVGFS 481
           LSKG  L+LPANNYLIRMD+AG++CLAFL TTSSLSIIGSFQQQG+RVSYDLVNSLVGFS
Sbjct: 429 LSKGKLLQLPANNYLIRMDTAGSYCLAFLKTTSSLSIIGSFQQQGMRVSYDLVNSLVGFS 481

Query: 482 SNKC 486
           SNKC
Sbjct: 489 SNKC 481

[Close]

BLAST of Spg022554.1 vs. ExPASy TrEMBL
Match: A0A5A7UQC2 (Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003690 PE=4 SV=1)

HSP 1 Score: 676.0 bits (1743), Expect = 1.2e-190
Identity = 358/488 (73.36%), Postives = 406/488 (83.20%), Query Frame = 0


Query: 8   SIFLFLTLLNSSFFSSSLSRLLTESPHSTTLLDVSASSKRAQNALSINPVQI-LHSHSFP 67
           S+FLFLT+  S  FSS LSR LT+SP+ST++ DV AS+ +A NALSI P  +  HSH   
Sbjct: 7   SVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQTHSHL-- 66

Query: 68  ILNPNSSLSLPLHPRLAIHKPSPSYKDYDSLVRARLARDAARVRSLNRNLQLALTGA--- 127
              PNSSLSLPL+PRL++H  +PSYKDYDSLVRARLARDAARV+ LNRNL+ +L G    
Sbjct: 67  ---PNSSLSLPLYPRLSLH--NPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDF 126

Query: 128 -----AVVRPDSITAPVVSGQSQGSG-EYFARIGVGQPAQSFYFVPDTGSDVTWLQCLPC 187
                  +  DSITAPVVSGQS+GSG EY A++GVGQP + FY VPDTGSDVTWLQC PC
Sbjct: 127 GEVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPC 186

Query: 188 SDANACYKQTDPIFDPKSSSSYSPLSCDSQQCQSLDRGSCQSGTCNYQVSYGDGSFTTGE 247
           +  NACYKQ DPIFDPKSSSSY+PLSC+SQQC  LDR +C SGTC YQV YGDGSFTTGE
Sbjct: 187 ATENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGE 246

Query: 248 FATETLSFANSKSVPNLPIGCGHDNEGLFVGAAGLIGLGGGDLSLSSQLKASSFSYCLVD 307
            ATETLSF NS S+PNLPIGCGHDNEGLF G AGLIGLGGG +SLSSQLKASSFSYCLV+
Sbjct: 247 LATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVN 306

Query: 308 RDSDSSSTLEFNSGRPSDSLTSPLLKNDRFGTYRYVEVTGMSVGGKPLPISSTRFDIDGS 367
            DSDSSSTLEFNS  PSDSLTSPL+KNDRF +YRYV+V G+SVGGK LPISSTRF+ID S
Sbjct: 307 MDSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVIGISVGGKTLPISSTRFEIDES 366

Query: 368 GLGGIIVDSGTFITRLPSDVYESLRDAFVNLTGNLTMTGGVSPFDTCYDFSGQSSVQVPT 427
           GLGGIIVDSGT I+RLPSDVYESLR+AFV LT +L+   G+S FDTCY+ S QS+V+VPT
Sbjct: 367 GLGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSSQSNVEVPT 426

Query: 428 VAFELSKGSSLRLPANNYLIRMDSAGTFCLAFLATTSSLSIIGSFQQQGIRVSYDLVNSL 486
           +AF LS G+SLRLPA NYLIR+D+AGT+CLAF+ T SSLSIIGSFQQQGIRVSYDL NSL
Sbjct: 427 IAFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSL 486

[Close]

BLAST of Spg022554.1 vs. ExPASy TrEMBL
Match: A0A5D3DYG2 (Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00370 PE=4 SV=1)

HSP 1 Score: 674.5 bits (1739), Expect = 3.4e-190
Identity = 357/488 (73.16%), Postives = 405/488 (82.99%), Query Frame = 0


Query: 8   SIFLFLTLLNSSFFSSSLSRLLTESPHSTTLLDVSASSKRAQNALSINPVQI-LHSHSFP 67
           S+FLFLT+  S  FSS LSR LT+SP+ST++ DV AS+ +A NALSI P  +  HSH   
Sbjct: 7   SVFLFLTIFTSLQFSSILSRKLTQSPYSTSIFDVLASTNQALNALSIKPKHLQTHSHL-- 66

Query: 68  ILNPNSSLSLPLHPRLAIHKPSPSYKDYDSLVRARLARDAARVRSLNRNLQLALTGA--- 127
              PNSSLSLPL+PRL++H  +PSYKDYDSLVRARLARDAARV+ LNRNL+ +L G    
Sbjct: 67  ---PNSSLSLPLYPRLSLH--NPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGKDF 126

Query: 128 -----AVVRPDSITAPVVSGQSQGSG-EYFARIGVGQPAQSFYFVPDTGSDVTWLQCLPC 187
                  +  DSITAPVVSGQS+GSG EY A++GVGQP + FY VPDTGSDVTWLQC PC
Sbjct: 127 GEVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQPC 186

Query: 188 SDANACYKQTDPIFDPKSSSSYSPLSCDSQQCQSLDRGSCQSGTCNYQVSYGDGSFTTGE 247
           +  NACYKQ DPIFDPKSSSSY+PLSC+SQQC  LDR +C SGTC YQV YGDGSFTTGE
Sbjct: 187 ATENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTTGE 246

Query: 248 FATETLSFANSKSVPNLPIGCGHDNEGLFVGAAGLIGLGGGDLSLSSQLKASSFSYCLVD 307
            ATE LSF NS S+PNLPIGCGHDNEGLF G AGLIGLGGG +SLSSQLKASSFSYCLV+
Sbjct: 247 LATEILSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCLVN 306

Query: 308 RDSDSSSTLEFNSGRPSDSLTSPLLKNDRFGTYRYVEVTGMSVGGKPLPISSTRFDIDGS 367
            DSDSSSTLEFNS  PSDSLTSPL+KNDRF +YRYV+V G+SVGG  LPISSTRF+ID S
Sbjct: 307 MDSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGNTLPISSTRFEIDES 366

Query: 368 GLGGIIVDSGTFITRLPSDVYESLRDAFVNLTGNLTMTGGVSPFDTCYDFSGQSSVQVPT 427
           GLGGIIVDSGT I+RLPSDVYESLR+AFV LT +L+   G+S FDTCY+ SGQS+V+VPT
Sbjct: 367 GLGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSGQSNVEVPT 426

Query: 428 VAFELSKGSSLRLPANNYLIRMDSAGTFCLAFLATTSSLSIIGSFQQQGIRVSYDLVNSL 486
           +AF LS G+SLRLPA NYLIR+D+AGT+CLAF+ T SSLSIIGSFQQQGIRVSYDL NSL
Sbjct: 427 IAFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTNSL 486

[Close]

BLAST of Spg022554.1 vs. ExPASy TrEMBL
Match: A0A0A0LPJ3 (Aspartic proteinase nepenthesin-1 OS=Cucumis sativus OX=3659 GN=Csa_1G022490 PE=4 SV=1)

HSP 1 Score: 662.5 bits (1708), Expect = 1.3e-186
Identity = 352/493 (71.40%), Postives = 402/493 (81.54%), Query Frame = 0


Query: 3   NNCFFSIFLFLTLLNSSFFSSSLSRLLTESPHSTTLLDVSASSKRAQNALSINPVQIL-H 62
           N    S+FLFLT+  S  F S LSR LT S +ST++ DVSAS+ +A +ALSI P  +  H
Sbjct: 2   NTSLSSVFLFLTIFTSLQFPSILSRKLTPSSYSTSIFDVSASTNQALDALSIKPKPLQNH 61

Query: 63  SHSFPILNPNSSLSLPLHPRLAIHKPSPSYKDYDSLVRARLARDAARVRSLNRNLQLALT 122
           SH      PNS  SLPL+PRLA+H  +PSYKDY++LVRARL RDAARV+ LNRNL+ +L 
Sbjct: 62  SHL-----PNSPFSLPLYPRLALH--NPSYKDYNTLVRARLTRDAARVQFLNRNLERSLN 121

Query: 123 GAA--------VVRPDSITAPVVSGQSQGSG-EYFARIGVGQPAQSFYFVPDTGSDVTWL 182
           G           +  DSITAPVVSGQS+GSG EY A+IGVGQP + FY VPDTGSDVTWL
Sbjct: 122 GGTHFGESINESLIGDSITAPVVSGQSKGSGAEYLAQIGVGQPVKLFYLVPDTGSDVTWL 181

Query: 183 QCLPCSDANACYKQTDPIFDPKSSSSYSPLSCDSQQCQSLDRGSCQSGTCNYQVSYGDGS 242
           QC PC+  N CYKQ DPIFDPKSSSSYSPLSC+SQQC+ LD+ +C S TC YQV YGDGS
Sbjct: 182 QCQPCASENTCYKQFDPIFDPKSSSSYSPLSCNSQQCKLLDKANCNSDTCIYQVHYGDGS 241

Query: 243 FTTGEFATETLSFANSKSVPNLPIGCGHDNEGLFVGAAGLIGLGGGDLSLSSQLKASSFS 302
           FTTGE ATETLSF NS S+PNLPIGCGHDNEGLF G AGLIGLGGG +SLSSQLKASSFS
Sbjct: 242 FTTGELATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFS 301

Query: 303 YCLVDRDSDSSSTLEFNSGRPSDSLTSPLLKNDRFGTYRYVEVTGMSVGGKPLPISSTRF 362
           YCLV+ DSDSSSTLEFNS  PSDSLTSPL+KNDRF +YRYV+V G+SVGGK LPIS TRF
Sbjct: 302 YCLVNLDSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVVGISVGGKTLPISPTRF 361

Query: 363 DIDGSGLGGIIVDSGTFITRLPSDVYESLRDAFVNLTGNLTMTGGVSPFDTCYDFSGQSS 422
           +ID SGLGGIIVDSGT I+RLPSDVYESLR+AFV LT +L+   G+S FDTCY+FSGQS+
Sbjct: 362 EIDESGLGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNFSGQSN 421

Query: 423 VQVPTVAFELSKGSSLRLPANNYLIRMDSAGTFCLAFLATTSSLSIIGSFQQQGIRVSYD 482
           V+VPT+AF LS+G+SLRLPA NYLI +D+AGT+CLAF+ T SSLSIIGSFQQQGIRVSYD
Sbjct: 422 VEVPTIAFVLSEGTSLRLPARNYLIMLDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYD 481

Query: 483 LVNSLVGFSSNKC 486
           L NSLVGFS+NKC
Sbjct: 482 LTNSLVGFSTNKC 487

[Close]

BLAST of Spg022554.1 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 468.0 bits (1203), Expect = 9.2e-132
Identity = 258/491 (52.55%), Postives = 339/491 (69.04%), Query Frame = 0


Query: 7   FSIFLFLTLLNSSFFSSSLSRLLTESPHSTT-LLDVSASSKRAQNALSINPVQILHSHSF 66
           +S F F+  L S   SS  SR+L E+  +TT +L+V+ S  R +   S      L+    
Sbjct: 5   YSFFFFIFFLTS--HSSVFSRILPETSTTTTSILNVADSIHRTKYTSSFR----LNQQEE 64

Query: 67  PILNPNSSLSLPLHPRLAIHKPSPSYKDYDSLVRARLARDAARVRSLNRNLQLALT--GA 126
              + +SS SL LH R+++      + DY SL  ARL RD ARV+SL   L LA+     
Sbjct: 65  QTHSASSSFSLQLHSRVSVR--GTEHSDYKSLTLARLNRDTARVKSLITRLDLAINNISK 124

Query: 127 AVVRPDS---------ITAPVVSGQSQGSGEYFARIGVGQPAQSFYFVPDTGSDVTWLQC 186
           A ++P S         I AP++SG +QGSGEYF R+G+G+PA+  Y V DTGSDV WLQC
Sbjct: 125 ADLKPISTMYTTEEQDIEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSDVNWLQC 184

Query: 187 LPCSDANACYKQTDPIFDPKSSSSYSPLSCDSQQCQSLDRGSCQSGTCNYQVSYGDGSFT 246
            PC+D   CY QT+PIF+P SSSSY PLSCD+ QC +L+   C++ TC Y+VSYGDGS+T
Sbjct: 185 TPCAD---CYHQTEPIFEPSSSSSYEPLSCDTPQCNALEVSECRNATCLYEVSYGDGSYT 244

Query: 247 TGEFATETLSFANSKSVPNLPIGCGHDNEGLFVGAAGLIGLGGGDLSLSSQLKASSFSYC 306
            G+FATETL+   S  V N+ +GCGH NEGLFVGAAGL+GLGGG L+L SQL  +SFSYC
Sbjct: 245 VGDFATETLTI-GSTLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTTSFSYC 304

Query: 307 LVDRDSDSSSTLEFNSGRPSDSLTSPLLKNDRFGTYRYVEVTGMSVGGKPLPISSTRFDI 366
           LVDRDSDS+ST++F +    D++ +PLL+N +  T+ Y+ +TG+SVGG+ L I  + F++
Sbjct: 305 LVDRDSDSASTVDFGTSLSPDAVVAPLLRNHQLDTFYYLGLTGISVGGELLQIPQSSFEM 364

Query: 367 DGSGLGGIIVDSGTFITRLPSDVYESLRDAFVNLTGNLTMTGGVSPFDTCYDFSGQSSVQ 426
           D SG GGII+DSGT +TRL +++Y SLRD+FV  T +L    GV+ FDTCY+ S +++V+
Sbjct: 365 DESGSGGIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTTVE 424

Query: 427 VPTVAFELSKGSSLRLPANNYLIRMDSAGTFCLAFLATTSSLSIIGSFQQQGIRVSYDLV 486
           VPTVAF    G  L LPA NY+I +DS GTFCLAF  T SSL+IIG+ QQQG RV++DL 
Sbjct: 425 VPTVAFHFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTFDLA 483

[Close]

BLAST of Spg022554.1 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 454.5 bits (1168), Expect = 1.1e-127
Identity = 251/499 (50.30%), Postives = 338/499 (67.74%), Query Frame = 0


Query: 8   SIFLFLTLLNSSFFSSSLSRLLTESPHSTTLLDVSASSKRAQNALSINPVQILHSHSFP- 67
           ++ LFLT  ++S  S SLS     +P  T +LDV +S ++ Q  LS++P +   + + P 
Sbjct: 14  TLSLFLTTTDAS--SRSLS-----TPPKTNVLDVVSSLQQTQTILSLDPTRSSLTTTKPE 73

Query: 68  ------ILNPNSSLSLPLHPRLAIHKPSPSYKDYDSLVRARLARDAARVRSLNRNLQLAL 127
                   N +S LSL LH R      +  +KDY SL  +RL RD++RV  +   ++ A+
Sbjct: 74  SLSDPVFFNSSSPLSLELHSRDTF--VASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAV 133

Query: 128 TGA--AVVRP----------DSITAPVVSGQSQGSGEYFARIGVGQPAQSFYFVPDTGSD 187
            G   + ++P          + +T PVVSG SQGSGEYF+RIGVG PA+  Y V DTGSD
Sbjct: 134 EGVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSD 193

Query: 188 VTWLQCLPCSDANACYKQTDPIFDPKSSSSYSPLSCDSQQCQSLDRGSCQSGTCNYQVSY 247
           V W+QC PC+D   CY+Q+DP+F+P SSS+Y  L+C + QC  L+  +C+S  C YQVSY
Sbjct: 194 VNWIQCEPCAD---CYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSY 253

Query: 248 GDGSFTTGEFATETLSFANSKSVPNLPIGCGHDNEGLFVGAAGLIGLGGGDLSLSSQLKA 307
           GDGSFT GE AT+T++F NS  + N+ +GCGHDNEGLF GAAGL+GLGGG LS+++Q+KA
Sbjct: 254 GDGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKA 313

Query: 308 SSFSYCLVDRDSDSSSTLEFNSGR-PSDSLTSPLLKNDRFGTYRYVEVTGMSVGGKPLPI 367
           +SFSYCLVDRDS  SS+L+FNS +      T+PLL+N +  T+ YV ++G SVGG+ + +
Sbjct: 314 TSFSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVL 373

Query: 368 SSTRFDIDGSGLGGIIVDSGTFITRLPSDVYESLRDAFVNLTGNLTM-TGGVSPFDTCYD 427
               FD+D SG GG+I+D GT +TRL +  Y SLRDAF+ LT NL   +  +S FDTCYD
Sbjct: 374 PDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYD 433

Query: 428 FSGQSSVQVPTVAFELSKGSSLRLPANNYLIRMDSAGTFCLAFLATTSSLSIIGSFQQQG 486
           FS  S+V+VPTVAF  + G SL LPA NYLI +D +GTFC AF  T+SSLSIIG+ QQQG
Sbjct: 434 FSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQG 493

[Close]

BLAST of Spg022554.1 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 360.1 bits (923), Expect = 2.7e-99
Identity = 187/409 (45.72%), Postives = 259/409 (63.33%), Query Frame = 0


Query: 87  PSPSYKDYDSLVRARLARDAARVRSLNRNLQLALTGAAVVRPDS------ITAPVVSGQS 146
           PS +Y+++   + AR+ RD  RV ++ R     ++G  +   DS        + +VSG  
Sbjct: 70  PSVTYRNHHHRLHARMRRDTDRVSAILRR----ISGKVIPSSDSRYEVNDFGSDIVSGMD 129

Query: 147 QGSGEYFARIGVGQPAQSFYFVPDTGSDVTWLQCLPCSDANACYKQTDPIFDPKSSSSYS 206
           QGSGEYF RIGVG P +  Y V D+GSD+ W+QC PC     CYKQ+DP+FDP  S SY+
Sbjct: 130 QGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPC---KLCYKQSDPVFDPAKSGSYT 189

Query: 207 PLSCDSQQCQSLDRGSCQSGTCNYQVSYGDGSFTTGEFATETLSFANSKSVPNLPIGCGH 266
            +SC S  C  ++   C SG C Y+V YGDGS+T G  A ETL+FA +  V N+ +GCGH
Sbjct: 190 GVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTFAKT-VVRNVAMGCGH 249

Query: 267 DNEGLFVGAAGLIGLGGGDLSLSSQLK---ASSFSYCLVDRDSDSSSTLEF-NSGRPSDS 326
            N G+F+GAAGL+G+GGG +S   QL      +F YCLV R +DS+ +L F     P  +
Sbjct: 250 RNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDSTGSLVFGREALPVGA 309

Query: 327 LTSPLLKNDRFGTYRYVEVTGMSVGGKPLPISSTRFDIDGSGLGGIIVDSGTFITRLPSD 386
              PL++N R  ++ YV + G+ VGG  +P+    FD+  +G GG+++D+GT +TRLP+ 
Sbjct: 310 SWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMDTGTAVTRLPTA 369

Query: 387 VYESLRDAFVNLTGNLTMTGGVSPFDTCYDFSGQSSVQVPTVAFELSKGSSLRLPANNYL 446
            Y + RD F + T NL    GVS FDTCYD SG  SV+VPTV+F  ++G  L LPA N+L
Sbjct: 370 AYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPARNFL 429

Query: 447 IRMDSAGTFCLAFLATTSSLSIIGSFQQQGIRVSYDLVNSLVGFSSNKC 486
           + +D +GT+C AF A+ + LSIIG+ QQ+GI+VS+D  N  VGF  N C
Sbjct: 430 MPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

[Close]

BLAST of Spg022554.1 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 359.8 bits (922), Expect = 3.5e-99
Identity = 233/509 (45.78%), Postives = 298/509 (58.55%), Query Frame = 0


Query: 3   NNCFFSIF--LFLTLLNSSFFSSSLSRLLTES-----PHSTTLLDVSASSKRAQNALSIN 62
           N   FS+F  LF T   SS + + +   L  S     P S +L D S S      ++ ++
Sbjct: 7   NTLAFSVFAVLFFTSSASSQYQTLVVNTLPSSATLSWPESESLTDESLSESTTSLSVHLS 66

Query: 63  PVQILHSHSFPILNPNSSLSLPLHPRLAIHKPSPSYKDYDSLVRARLARDAARVRSLNRN 122
            V  L S S                       SP+      L   RL RD+ RV+S+  +
Sbjct: 67  HVDALSSFS---------------------DASPA-----DLFNLRLQRDSLRVKSIT-S 126

Query: 123 LQLALTGAAVVRPDSITA-----PVVSGQSQGSGEYFARIGVGQPAQSFYFVPDTGSDVT 182
           L    TG    +    TA      V+SG SQGSGEYF R+GVG PA + Y V DTGSDV 
Sbjct: 127 LAAVSTGRNATKRTPRTAGGFSGAVISGLSQGSGEYFMRLGVGTPATNVYMVLDTGSDVV 186

Query: 183 WLQCLPCSDANACYKQTDPIFDPKSSSSYSPLSCDSQQCQSLDRGS-C---QSGTCNYQV 242
           WLQC PC    ACY QTD IFDPK S +++ + C S+ C+ LD  S C   +S TC YQV
Sbjct: 187 WLQCSPC---KACYNQTDAIFDPKKSKTFATVPCGSRLCRRLDDSSECVTRRSKTCLYQV 246

Query: 243 SYGDGSFTTGEFATETLSFANSKSVPNLPIGCGHDNEGLFVGAAGLIGLGGGDLSLSSQL 302
           SYGDGSFT G+F+TETL+F  ++ V ++P+GCGHDNEGLFVGAAGL+GLG G LS  SQ 
Sbjct: 247 SYGDGSFTEGDFSTETLTFHGAR-VDHVPLGCGHDNEGLFVGAAGLLGLGRGGLSFPSQT 306

Query: 303 K---ASSFSYCLVDRDSDSS-----STLEF-NSGRPSDSLTSPLLKNDRFGTYRYVEVTG 362
           K      FSYCLVDR S  S     ST+ F N+  P  S+ +PLL N +  T+ Y+++ G
Sbjct: 307 KNRYNGKFSYCLVDRTSSGSSSKPPSTIVFGNAAVPKTSVFTPLLTNPKLDTFYYLQLLG 366

Query: 363 MSVGGKPLP-ISSTRFDIDGSGLGGIIVDSGTFITRLPSDVYESLRDAFVNLTGNLTMTG 422
           +SVGG  +P +S ++F +D +G GG+I+DSGT +TRL    Y +LRDAF      L    
Sbjct: 367 ISVGGSRVPGVSESQFKLDATGNGGVIIDSGTSVTRLTQPAYVALRDAFRLGATKLKRAP 426

Query: 423 GVSPFDTCYDFSGQSSVQVPTVAFELSKGSSLRLPANNYLIRMDSAGTFCLAFLATTSSL 482
             S FDTC+D SG ++V+VPTV F    G  + LPA+NYLI +++ G FC AF  T  SL
Sbjct: 427 SYSLFDTCFDLSGMTTVKVPTVVFHFG-GGEVSLPASNYLIPVNTEGRFCFAFAGTMGSL 483

Query: 483 SIIGSFQQQGIRVSYDLVNSLVGFSSNKC 486
           SIIG+ QQQG RV+YDLV S VGF S  C
Sbjct: 487 SIIGNIQQQGFRVAYDLVGSRVGFLSRAC 483

[Close]

BLAST of Spg022554.1 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 348.2 bits (892), Expect = 1.1e-95
Identity = 198/407 (48.65%), Postives = 254/407 (62.41%), Query Frame = 0


Query: 90  SYKDYDSLVRARLARDAARVRSLNRNLQLALTGAAVV---RPDSITAPVVSGQSQGSGEY 149
           S K  D L  +RL RD+ RV+S+   L   + G  V    RP   ++ VVSG SQGSGEY
Sbjct: 84  SNKTPDELFSSRLQRDSRRVKSI-ATLAAQIPGRNVTHAPRPGGFSSSVVSGLSQGSGEY 143

Query: 150 FARIGVGQPAQSFYFVPDTGSDVTWLQCLPCSDANACYKQTDPIFDPKSSSSYSPLSCDS 209
           F R+GVG PA+  Y V DTGSD+ WLQC PC     CY Q+DPIFDP+ S +Y+ + C S
Sbjct: 144 FTRLGVGTPARYVYMVLDTGSDIVWLQCAPC---RRCYSQSDPIFDPRKSKTYATIPCSS 203

Query: 210 QQCQSLDRGSCQS--GTCNYQVSYGDGSFTTGEFATETLSFANSKSVPNLPIGCGHDNEG 269
             C+ LD   C +   TC YQVSYGDGSFT G+F+TETL+F  ++ V  + +GCGHDNEG
Sbjct: 204 PHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNR-VKGVALGCGHDNEG 263

Query: 270 LFVGAAGLIGLGGGDLSLSSQLK---ASSFSYCLVDRDSDS--SSTLEFNSGRPSDSLTS 329
           LFVGAAGL+GLG G LS   Q        FSYCLVDR + S  SS +  N+     +  +
Sbjct: 264 LFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNAAVSRIARFT 323

Query: 330 PLLKNDRFGTYRYVEVTGMSVGGKPLP-ISSTRFDIDGSGLGGIIVDSGTFITRLPSDVY 389
           PLL N +  T+ YV + G+SVGG  +P ++++ F +D  G GG+I+DSGT +TRL    Y
Sbjct: 324 PLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAY 383

Query: 390 ESLRDAFVNLTGNLTMTGGVSPFDTCYDFSGQSSVQVPTVAFELSKGSSLRLPANNYLIR 449
            ++RDAF      L      S FDTC+D S  + V+VPTV     +G+ + LPA NYLI 
Sbjct: 384 IAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHF-RGADVSLPATNYLIP 443

Query: 450 MDSAGTFCLAFLATTSSLSIIGSFQQQGIRVSYDLVNSLVGFSSNKC 486
           +D+ G FC AF  T   LSIIG+ QQQG RV YDL +S VGF+   C
Sbjct: 444 VDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGC 484

The following BLAST results are available for this feature:

BLAST of Spg022554.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023531915.1	6.5e-204	78.72	protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita pepo subsp. pepo]	[more]
KAG7021604.1	2.5e-203	78.51	Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp...	[more]
XP_023007215.1	3.2e-203	78.97	protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita maxima]	[more]
XP_022933467.1	1.6e-202	78.10	protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita moschata] >KAG6587644....	[more]
XP_038878113.1	6.3e-191	73.98	protein ASPARTIC PROTEASE IN GUARD CELL 1 [Benincasa hispida]	[more]

BLAST of Spg022554.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LS40	1.5e-126	50.30	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LHE3	3.8e-98	45.72	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LNJ3	1.5e-94	48.65	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q766C3	7.0e-68	41.71	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q766C2	4.5e-67	40.51	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]

BLAST of Spg022554.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1L2D2	1.6e-203	78.97	protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1EZU8	7.7e-203	78.10	protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A5A7UQC2	1.2e-190	73.36	Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=11...	[more]
A0A5D3DYG2	3.4e-190	73.16	Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=11...	[more]
A0A0A0LPJ3	1.3e-186	71.40	Aspartic proteinase nepenthesin-1 OS=Cucumis sativus OX=3659 GN=Csa_1G022490 PE=...	[more]

BLAST of Spg022554.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G25510.1	9.2e-132	52.55	Eukaryotic aspartyl protease family protein	[more]
AT3G18490.1	1.1e-127	50.30	Eukaryotic aspartyl protease family protein	[more]
AT3G20015.1	2.7e-99	45.72	Eukaryotic aspartyl protease family protein	[more]
AT3G61820.1	3.5e-99	45.78	Eukaryotic aspartyl protease family protein	[more]
AT1G01300.1	1.1e-95	48.65	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 310..485 e-value: 9.1E-48 score: 164.3
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 121..308 e-value: 3.0E-51 score: 176.2
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 139..485
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 146..308 e-value: 2.5E-48 score: 164.7
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 332..481 e-value: 2.2E-36 score: 125.1
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 17..485
None	No IPR available	PANTHER	PTHR13683:SF274	PROTEIN ASPARTIC PROTEASE IN GUARD CELL 1	coord: 17..485
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 146..481 score: 45.978207

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spg022554	Spg022554	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spg022554.1-cds	Spg022554.1-cds-scaffold2:3731372..3732829	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spg022554.1	Spg022554.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016020	membrane
molecular_function	GO:0004190	aspartic-type endopeptidase activity