Spg008623.1 (mRNA) Sponge gourd (cylindrica) v1

BLAST of Spg008623.1 vs. NCBI nr
Match: XP_038884306.1 (LOW QUALITY PROTEIN: putative wall-associated receptor kinase-like 16 [Benincasa hispida])

HSP 1 Score: 275.0 bits (702), Expect = 5.9e-70
Identity = 130/225 (57.78%), Postives = 168/225 (74.67%), Query Frame = 0

Query: 6   EALVRRLVVLKISILAVAAAALAASQAKPGCDDMCGNLTIPYPFGMSKDCYLDEAFFILC 65
           + LVR ++V+ I+I +  AAA   SQA PGC+  CG+L IPYPFGM K CYL+  F I C
Sbjct: 7   KTLVRLIMVVNIAIFSAPAAAAVPSQALPGCESKCGDLEIPYPFGMKKGCYLNINFSITC 66

Query: 66  NKTHYDPPMAFLTGGNIEITNISILHGELHILHYVARDCYAQDGRNIKNDKPTLSLANFT 125
           N+THY PP  FL  GNIE+TNIS L+GELHIL+Y+ARDCY +DG    N++P L++  F 
Sbjct: 67  NETHYHPPKPFLMNGNIEVTNIS-LYGELHILNYIARDCYRKDGLPGINNRPRLTVPMFA 126

Query: 126 ISNTKNKFTVIGCDTYAYIYGQLEGEKFRTACVASCDDISQV--KDGSCSGFGCCQLQIP 185
           ISNTKNKFTV+GCDTYAYIYG L GE + + C+A C   S V  +DGSCSG GCCQL+IP
Sbjct: 127 ISNTKNKFTVVGCDTYAYIYGDLPGESYTSGCMALCTAESDVTIEDGSCSGNGCCQLEIP 186

Query: 186 KVLKDLKYEVYSFKNHSEVQDFNPCGYAFLIEEDMFNFSKTFIRD 229
           K LK L+ +V SF NH+ V  FNPCG+AF+++++ F+FSK +I++
Sbjct: 187 KGLKLLRLDVRSFDNHTYVHSFNPCGFAFVVQQNQFSFSKKYIQN 230

BLAST of Spg008623.1 vs. NCBI nr
Match: KAG6602140.1 (Wall-associated receptor kinase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 272.3 bits (695), Expect = 3.8e-69
Identity = 131/234 (55.98%), Postives = 172/234 (73.50%), Query Frame = 0

Query: 1   MGGSMEALVRRLV--VLKISILAVAAAALAASQAKPGCDDMCGNLTIPYPFGMSKDCYLD 60
           MG  +E L+R ++  +L +S+L  AA A+AASQA PGCDD CG+L IPYPFG  + CYL+
Sbjct: 1   MGRQIETLIRLMIANILILSLLVYAATAVAASQALPGCDDRCGDLQIPYPFGTREGCYLN 60

Query: 61  EAFFILCNKTHYDPPMAFLTGGNIEITNISILHGELHILHYVARDCYAQDGRNIKNDK-- 120
           + F I C+ THYD P  FL  GNI++TNISI  GELHILH+ A+ CY  +  NI ++   
Sbjct: 61  KNFLITCDTTHYDRPTPFLRTGNIQVTNISI-SGELHILHFTAKYCYPMN--NILDESWT 120

Query: 121 -PTLSLANFTISNTKNKFTVIGCDTYAYIYGQLEGEKFRTACVASCDDISQVKDGSCSGF 180
             TL L+ FT+S++KNKFTVIGCDTYAY+ G +EG+ + T C+A C++I+ V+DG+CSG 
Sbjct: 121 FATLDLSEFTVSSSKNKFTVIGCDTYAYLSGLIEGQNYTTGCMAWCENINSVRDGACSGN 180

Query: 181 GCCQLQIPKVLKDLKYEVYSFKNHSEVQDFNPCGYAFLIEEDMFNFSKTFIRDF 230
           GCCQL IP  LK L+  V+SF NH+ V  FNPCGYAF+ EED FNFS+ +IR+F
Sbjct: 181 GCCQLDIPSGLKGLRCTVHSFHNHTNVLSFNPCGYAFVTEEDKFNFSRAYIRNF 231

BLAST of Spg008623.1 vs. NCBI nr
Match: XP_022989770.1 (wall-associated receptor kinase 2-like [Cucurbita maxima])

HSP 1 Score: 271.2 bits (692), Expect = 8.5e-69
Identity = 128/220 (58.18%), Postives = 167/220 (75.91%), Query Frame = 0

Query: 11  RLVVLKISILAVAAAALAASQAKPGCDDMCGNLTIPYPFGMSKDCYLDEAFFILCNKTHY 70
           RL+++ I IL+ +  A+ ASQA  GCDD CG+L IPYPFG  + CYL++ F I CN TH+
Sbjct: 10  RLMIINILILSPSVYAV-ASQALLGCDDRCGDLQIPYPFGTREGCYLNKNFLITCNTTHF 69

Query: 71  DPPMAFLTGGNIEITNISILHGELHILHYVARDCYAQDGR-NIKNDKPTLSLANFTISNT 130
           DPP  FL  GNI++TNISI  GEL ILH+ A+DCY ++   + +    TL+L+ FT+S+T
Sbjct: 70  DPPRPFLRTGNIQVTNISI-SGELQILHFGAKDCYPKNNSLDARRTSATLNLSTFTVSST 129

Query: 131 KNKFTVIGCDTYAYIYGQLEGEKFRTACVASCDDISQVKDGSCSGFGCCQLQIPKVLKDL 190
           KNKFTVIGCDTYA++ GQ+EG+ +RTACVA CD+I+ V+DG+CSG GCCQL IP  LK L
Sbjct: 130 KNKFTVIGCDTYAFLSGQIEGQSYRTACVALCDNITTVRDGACSGNGCCQLDIPSGLKGL 189

Query: 191 KYEVYSFKNHSEVQDFNPCGYAFLIEEDMFNFSKTFIRDF 230
           +Y V SF NH++V  FNPCGYAF+ EED F+FS  +IR+F
Sbjct: 190 RYRVRSFDNHTDVFSFNPCGYAFVTEEDKFHFSAAYIRNF 227

BLAST of Spg008623.1 vs. NCBI nr
Match: XP_023512416.1 (wall-associated receptor kinase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 270.4 bits (690), Expect = 1.4e-68
Identity = 127/226 (56.19%), Postives = 166/226 (73.45%), Query Frame = 0

Query: 5   MEALVRRLVVLKISILAVAAAALAASQAKPGCDDMCGNLTIPYPFGMSKDCYLDEAFFIL 64
           ME  +  L+ L I  + + ++ + ASQA  GC D CG+L IPYPFG  + CYL++ F I 
Sbjct: 1   MERQIETLIRLMIVNILILSSPVYASQALLGCPDRCGDLQIPYPFGTREGCYLNKNFLIT 60

Query: 65  CNKTHYDPPMAFLTGGNIEITNISILHGELHILHYVARDCYAQDGR-NIKNDKPTLSLAN 124
           CN TH+DPP  FL  GNI++TNISI  GEL ILH+ A+DCY ++   + +    TL+L+ 
Sbjct: 61  CNTTHFDPPRPFLRTGNIQVTNISI-SGELQILHFGAKDCYPKNNSVDARRTSATLNLST 120

Query: 125 FTISNTKNKFTVIGCDTYAYIYGQLEGEKFRTACVASCDDISQVKDGSCSGFGCCQLQIP 184
           FT+S+TKNKFTVIGCDTYA++ GQ+EG+ +RTACVA CD+I+ V+DG+CSG GCCQL IP
Sbjct: 121 FTVSSTKNKFTVIGCDTYAFLSGQIEGQSYRTACVALCDNITTVRDGACSGNGCCQLDIP 180

Query: 185 KVLKDLKYEVYSFKNHSEVQDFNPCGYAFLIEEDMFNFSKTFIRDF 230
             LK L+Y V SF NH++V  FNPCGYAF+ EED F+FS  +IRDF
Sbjct: 181 SGLKGLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDF 225

BLAST of Spg008623.1 vs. NCBI nr
Match: XP_038886240.1 (wall-associated receptor kinase 2-like [Benincasa hispida])

HSP 1 Score: 267.7 bits (683), Expect = 9.4e-68
Identity = 124/230 (53.91%), Postives = 167/230 (72.61%), Query Frame = 0

Query: 5   MEALVRRLVVLKISILAVAAAALAASQAKPGCDDMCGNLTIPYPFGMSKDCYLDEAFFIL 64
           ME    +L+ L + I+  + A   A+ A   C+  CGNL IPYPFGM K CYL+E F + 
Sbjct: 1   MEHPEEKLLFLFMLIMFPSVALATAAAASQDCERQCGNLEIPYPFGMKKGCYLNENFLVT 60

Query: 65  CNKTHYDPPMAFLTGGNIEITNISILHGELHILHYVARDCYAQDGR-NIKNDKPTLSLAN 124
           CNKTHY PP AFL G NIE+T+ISIL  ELHIL+YVARDCY +DGR +I   +P L +  
Sbjct: 61  CNKTHYHPPKAFLRGSNIEVTHISILPSELHILNYVARDCYTKDGRPDISRARPFLVVDM 120

Query: 125 FTISNTKNKFTVIGCDTYAYIYGQLEGEKFRTACVASCDDISQ-VKDGSCSGFGCCQLQI 184
           F+ISNTKNK T++GCDTY Y++G+++GE + + C+A C + S+ +KDGSCSG GCCQL+I
Sbjct: 121 FSISNTKNKLTIVGCDTYGYLHGEIQGEIYSSGCMALCGNSSRTIKDGSCSGSGCCQLEI 180

Query: 185 PKVLKDLKYEVYSFKNHSEVQDFNPCGYAFLIEEDMFNFSKTFIRDFVVS 233
           PK LK+++ +V SF NH+ V   NPCGYAF+I+++ F FSK +I++F  S
Sbjct: 181 PKGLKEIQLDVRSFNNHTRVHSLNPCGYAFVIQQNRFTFSKNYIKNFTES 230

BLAST of Spg008623.1 vs. ExPASy Swiss-Prot
Match: Q9LMP1 (Wall-associated receptor kinase 2 OS=Arabidopsis thaliana OX=3702 GN=WAK2 PE=1 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 8.3e-35
Identity = 79/208 (37.98%), Postives = 120/208 (57.69%), Query Frame = 0

Query: 17  ISILAVAAAALAASQAKPGCDDMCGNLTIPYPFGMSKDCYL--DEAFFILCNKTHYDPPM 76
           +++  +A   L   Q +  C   CGN+ + YPFG S  CY   DE+F + CN+       
Sbjct: 10  VAVFYLAYTQLVKGQPRKECQTRCGNVAVEYPFGTSPGCYYPGDESFNLTCNEQEK---- 69

Query: 77  AFLTGGNIEITNISILHGELHILHYVARDCYAQDGRNIKNDKPTLSLANFTISNTKNKFT 136
             L  GN+ + N+S L G+L +    +R CY   G+         +L NFT+S   N+FT
Sbjct: 70  --LFFGNMPVINMS-LSGQLRVRLVRSRVCYDSQGKQTDYIAQRTTLGNFTLSEL-NRFT 129

Query: 137 VIGCDTYAYIYGQLEG-EKFRTACVASCDDISQVKDGSCSGFGCCQLQIPKVLKDLKYEV 196
           V+GC++YA++  +  G EK+ T C++ CD  +  K+GSCSG GCCQ+ +P+    ++ + 
Sbjct: 130 VVGCNSYAFL--RTSGVEKYSTGCISICDS-ATTKNGSCSGEGCCQIPVPRGYSFVRVKP 189

Query: 197 YSFKNHSEVQDFNPCGYAFLIEEDMFNF 222
           +SF NH  V  FNPC YAFL+E+ MF+F
Sbjct: 190 HSFHNHPTVHLFNPCTYAFLVEDGMFDF 206

BLAST of Spg008623.1 vs. ExPASy Swiss-Prot
Match: Q39191 (Wall-associated receptor kinase 1 OS=Arabidopsis thaliana OX=3702 GN=WAK1 PE=1 SV=2)

HSP 1 Score: 126.3 bits (316), Expect = 4.4e-28
Identity = 74/212 (34.91%), Postives = 116/212 (54.72%), Query Frame = 0

Query: 21  AVAAAALAASQAKPG--CDDMCGNLTIPYPFGMSKDCYL--DEAFFILCNKTHYDPPMAF 80
           ++A   L   Q +PG  C + CGN+TI YPFG+S  CY   +E+F I C +   D P   
Sbjct: 15  SLACTQLVKGQHQPGENCQNKCGNITIEYPFGISSGCYYPGNESFSITCKE---DRPHVL 74

Query: 81  LTGGNIEITNISILHGELHILHYVARDCYAQDGRNIKNDKPTLSLANFTISNTKNKFTVI 140
               +IE+ N +   G+L +L   +  CY + G+  + D  + +L N ++S   NK T +
Sbjct: 75  ---SDIEVANFN-HSGQLQVLLNRSSTCYDEQGKKTEEDS-SFTLENLSLS-ANNKLTAV 134

Query: 141 GCDTYAYI--YGQLEGEKFRTACVASCDDISQVKDGSCSGFGCCQLQIPKVLKDLKYEVY 200
           GC+  + +  +G    + + TAC++ CD   +  DG C+G GCC++ +   L    +E  
Sbjct: 135 GCNALSLLDTFGM---QNYSTACLSLCDSPPEA-DGECNGRGCCRVDVSAPLDSYTFETT 194

Query: 201 S--FKNHSEVQDFNPCGYAFLIEEDMFNFSKT 225
           S   K+ +   DF+PC YAFL+E+D FNFS T
Sbjct: 195 SGRIKHMTSFHDFSPCTYAFLVEDDKFNFSST 213

BLAST of Spg008623.1 vs. ExPASy Swiss-Prot
Match: Q9LMN7 (Wall-associated receptor kinase 5 OS=Arabidopsis thaliana OX=3702 GN=WAK5 PE=2 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 3.6e-22
Identity = 68/205 (33.17%), Postives = 103/205 (50.24%), Query Frame = 0

Query: 22  VAAAALAASQAKPGCDDMCGNLTIPYPFGMSKDCYL--DEAFFILCNKTHYDPPMAFLTG 81
           +A   L  +Q +  C   CG++ I YPFG+S  CY   D++F I C +   D P      
Sbjct: 15  LAYTQLVKAQPRDDCQTRCGDVPIDYPFGISTGCYYPGDDSFNITCEE---DKPNVL--- 74

Query: 82  GNIEITNISILHGELHILHYVARDCYAQDGRNIKNDKPTLSLANFTISNTKNKFTVIGCD 141
            NIE+ N +   G+L  L   +  CY Q   N   +     L N + S   NKFT++GC+
Sbjct: 75  SNIEVLNFN-HSGQLRGLIPRSTVCYDQQTNN-DFESLWFRLDNLSFS-PNNKFTLVGCN 134

Query: 142 TYAYIYGQLEGEKFRTACVASCDDISQVKDGSCSGFGCCQLQIPKVLKDLKYEVY--SFK 201
            +A +      + + T C++ C D     +  C+G GCC+ ++   L   + E     F+
Sbjct: 135 AWA-LLSTFGIQNYSTGCMSLC-DTPPPPNSKCNGVGCCRTEVSIPLDSHRIETQPSRFE 194

Query: 202 NHSEVQDFNPCGYAFLIEEDMFNFS 223
           N + V+ FNPC YAF +E+ MFNFS
Sbjct: 195 NMTSVEHFNPCSYAFFVEDGMFNFS 208

BLAST of Spg008623.1 vs. ExPASy Swiss-Prot
Match: Q9LMN6 (Wall-associated receptor kinase 4 OS=Arabidopsis thaliana OX=3702 GN=WAK4 PE=2 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 2.3e-21
Identity = 67/218 (30.73%), Postives = 107/218 (49.08%), Query Frame = 0

Query: 9   VRRLVVLKISILAVAAAALAASQAKPGCDDMCGNLTIPYPFGMSKDCYL--DEAFFILCN 68
           V+RL +  ++I  ++   L   Q  P C + CGN+T+ YPFG S  C+   D +F + C 
Sbjct: 3   VQRLFL--VAIFCLSYMQLVKGQTLPRCPEKCGNVTLEYPFGFSPGCWRAEDPSFNLSCV 62

Query: 69  KTHYDPPMAFLTGGNIEITNISILHGELHILHYVARDCYAQDGRNIKNDKPTLSLANFTI 128
             +      F  G  +E+  IS    +L +L+  +  CY   G+  K      +L N T+
Sbjct: 63  NEN-----LFYKG--LEVVEIS-HSSQLRVLYPASYICYNSKGKFAKGTYYWSNLGNLTL 122

Query: 129 SNTKNKFTVIGCDTYAYIYGQLEGEKFRTACVASCDDISQVKDGSCSGFGCCQLQIPKVL 188
           S   N  T +GC++YA++       +    C+++CD +S   +G C+G GCCQ  +P   
Sbjct: 123 SG-NNTITALGCNSYAFVSSN-GTRRNSVGCISACDALSHEANGECNGEGCCQNPVPAGN 182

Query: 189 KDLKYEVYSFKNHSEVQDFN--PCGYAFLIEEDMFNFS 223
             L    Y F N + VQ  +   C YAFL+E   F ++
Sbjct: 183 NWLIVRSYRFDNDTSVQPISEGQCIYAFLVENGKFKYN 208

BLAST of Spg008623.1 vs. ExPASy Swiss-Prot
Match: Q9LMN8 (Wall-associated receptor kinase 3 OS=Arabidopsis thaliana OX=3702 GN=WAK3 PE=2 SV=2)

HSP 1 Score: 94.7 bits (234), Expect = 1.4e-18
Identity = 67/227 (29.52%), Postives = 106/227 (46.70%), Query Frame = 0

Query: 17  ISILAVAAAALAASQAKPGCDDMCGNLTIPYPFGMSKDCYL--DEAFFILCNKTHYDPPM 76
           I  LA         Q +  C   CGN+TI YPFG+S  CY   D+ F + C         
Sbjct: 12  IFFLAYTQLVKGQHQPREDCKLKCGNVTIEYPFGISTGCYYPGDDNFNLTCVVEE----- 71

Query: 77  AFLTGGNIEITNISILHGELHILHYVARDCYAQDGRNIKNDKPTLSL-----ANFTISNT 136
             L  G I++TNIS   G + +L     +CY Q     KN+    +L     ++F++S +
Sbjct: 72  KLLLFGIIQVTNIS-HSGHVSVLFERFSECYEQ-----KNETNGTALGYQLGSSFSLS-S 131

Query: 137 KNKFTVIGCDTYAYIYGQLEGEKFRTACVASCDDISQVKDGSCSGFGCC---QLQIPKVL 196
            NKFT++GC+  + +      + + T C++ C+   +  +G C+G GCC      +P   
Sbjct: 132 NNKFTLVGCNALS-LLSTFGKQNYSTGCLSLCNSQPEA-NGRCNGVGCCTTEDFSVPFDS 191

Query: 197 KDLKYEVYSFKN---------HSEVQDFNPCGYAFLIEEDMFNFSKT 225
              ++     +N         ++ V  FNPC YAFL+E+  FNF  +
Sbjct: 192 DTFQFGSVRLRNQVNNSLDLFNTSVYQFNPCTYAFLVEDGKFNFDSS 224

BLAST of Spg008623.1 vs. ExPASy TrEMBL
Match: A0A6J1JNA5 (wall-associated receptor kinase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111486858 PE=4 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 4.1e-69
Identity = 128/220 (58.18%), Postives = 167/220 (75.91%), Query Frame = 0

Query: 11  RLVVLKISILAVAAAALAASQAKPGCDDMCGNLTIPYPFGMSKDCYLDEAFFILCNKTHY 70
           RL+++ I IL+ +  A+ ASQA  GCDD CG+L IPYPFG  + CYL++ F I CN TH+
Sbjct: 10  RLMIINILILSPSVYAV-ASQALLGCDDRCGDLQIPYPFGTREGCYLNKNFLITCNTTHF 69

Query: 71  DPPMAFLTGGNIEITNISILHGELHILHYVARDCYAQDGR-NIKNDKPTLSLANFTISNT 130
           DPP  FL  GNI++TNISI  GEL ILH+ A+DCY ++   + +    TL+L+ FT+S+T
Sbjct: 70  DPPRPFLRTGNIQVTNISI-SGELQILHFGAKDCYPKNNSLDARRTSATLNLSTFTVSST 129

Query: 131 KNKFTVIGCDTYAYIYGQLEGEKFRTACVASCDDISQVKDGSCSGFGCCQLQIPKVLKDL 190
           KNKFTVIGCDTYA++ GQ+EG+ +RTACVA CD+I+ V+DG+CSG GCCQL IP  LK L
Sbjct: 130 KNKFTVIGCDTYAFLSGQIEGQSYRTACVALCDNITTVRDGACSGNGCCQLDIPSGLKGL 189

Query: 191 KYEVYSFKNHSEVQDFNPCGYAFLIEEDMFNFSKTFIRDF 230
           +Y V SF NH++V  FNPCGYAF+ EED F+FS  +IR+F
Sbjct: 190 RYRVRSFDNHTDVFSFNPCGYAFVTEEDKFHFSAAYIRNF 227

BLAST of Spg008623.1 vs. ExPASy TrEMBL
Match: A0A5D3DKI3 (Wall-associated receptor kinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1607G001160 PE=4 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 4.5e-68
Identity = 131/227 (57.71%), Postives = 162/227 (71.37%), Query Frame = 0

Query: 5   MEALVRRLVVLKISILAVAAAALAASQAKPGCDDMCGNLTIPYPFGMSKDCYLDEAFFIL 64
           M+     L+ L I +    AAA+  ++   GCD+ CGNL IPYPFG+ K CYLD+AF I 
Sbjct: 1   MKIQAEDLMKLIILLSGTLAAAVEVAKVLTGCDEHCGNLRIPYPFGIKKRCYLDQAFSIT 60

Query: 65  CNKTHYDPPMAFLTGGNIEITNISILHGELHILHYVARDCYAQDGRNIKNDKPTLSLANF 124
           CNKT Y PP  FL   NIEITNISI+ G+LHI  +VARDCY ++G +  N +P L L  F
Sbjct: 61  CNKT-YHPPKPFLQDSNIEITNISIIQGQLHIKQFVARDCYTKNGPSESNTRPFLLLNKF 120

Query: 125 TISNTKNKFTVIGCDTYAYIYGQLEGEKFRTACVASC--DDISQVKDGSCSGFGCCQLQI 184
            ISNT NKF VIGCDTYAYIYG++EGE +++ C+A C  ++   +KDGSCSG GCCQLQI
Sbjct: 121 RISNTDNKFIVIGCDTYAYIYGEIEGESYKSGCMALCGNNNTKIIKDGSCSGNGCCQLQI 180

Query: 185 PKVLKDLKYEVYSFKNHSEVQDFNPCGYAFLIEEDMFNFSKTFIRDF 230
           PK LK L+ EV SF NHSEV  FNPCGYAF+I++D F FSK +I +F
Sbjct: 181 PKGLKILELEVRSFDNHSEVLKFNPCGYAFVIQQDKFTFSKKYIYNF 226

BLAST of Spg008623.1 vs. ExPASy TrEMBL
Match: A0A1S3B3R7 (wall-associated receptor kinase 2-like OS=Cucumis melo OX=3656 GN=LOC103485671 PE=4 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 4.5e-68
Identity = 131/227 (57.71%), Postives = 162/227 (71.37%), Query Frame = 0

Query: 5   MEALVRRLVVLKISILAVAAAALAASQAKPGCDDMCGNLTIPYPFGMSKDCYLDEAFFIL 64
           M+     L+ L I +    AAA+  ++   GCD+ CGNL IPYPFG+ K CYLD+AF I 
Sbjct: 1   MKIQAEDLMKLIILLSGTLAAAVEVAKVLTGCDEHCGNLRIPYPFGIKKRCYLDQAFSIT 60

Query: 65  CNKTHYDPPMAFLTGGNIEITNISILHGELHILHYVARDCYAQDGRNIKNDKPTLSLANF 124
           CNKT Y PP  FL   NIEITNISI+ G+LHI  +VARDCY ++G +  N +P L L  F
Sbjct: 61  CNKT-YHPPKPFLQDSNIEITNISIIQGQLHIKQFVARDCYTKNGPSESNTRPFLLLNKF 120

Query: 125 TISNTKNKFTVIGCDTYAYIYGQLEGEKFRTACVASC--DDISQVKDGSCSGFGCCQLQI 184
            ISNT NKF VIGCDTYAYIYG++EGE +++ C+A C  ++   +KDGSCSG GCCQLQI
Sbjct: 121 RISNTDNKFIVIGCDTYAYIYGEIEGESYKSGCMALCGNNNTKIIKDGSCSGNGCCQLQI 180

Query: 185 PKVLKDLKYEVYSFKNHSEVQDFNPCGYAFLIEEDMFNFSKTFIRDF 230
           PK LK L+ EV SF NHSEV  FNPCGYAF+I++D F FSK +I +F
Sbjct: 181 PKGLKILELEVRSFDNHSEVLKFNPCGYAFVIQQDKFTFSKKYIYNF 226

BLAST of Spg008623.1 vs. ExPASy TrEMBL
Match: A0A6J1H843 (wall-associated receptor kinase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111461403 PE=4 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 1.0e-67
Identity = 124/222 (55.86%), Postives = 164/222 (73.87%), Query Frame = 0

Query: 9   VRRLVVLKISILAVAAAALAASQAKPGCDDMCGNLTIPYPFGMSKDCYLDEAFFILCNKT 68
           +  L+ L I  + + ++ + ASQA  GC D CG+L IPYPFG  + CYL++ F I CN T
Sbjct: 712 IETLIRLMIVNILILSSPVYASQALLGCPDRCGDLQIPYPFGTREGCYLNKNFLITCNTT 771

Query: 69  HYDPPMAFLTGGNIEITNISILHGELHILHYVARDCYAQDGR-NIKNDKPTLSLANFTIS 128
           H++PP  FL  GNI++TNISI  GEL ILH+ A+DCY ++   + +    TL+L+ FT+S
Sbjct: 772 HFNPPRPFLRTGNIQVTNISI-SGELQILHFGAKDCYPKNNSLDARRTSATLNLSTFTVS 831

Query: 129 NTKNKFTVIGCDTYAYIYGQLEGEKFRTACVASCDDISQVKDGSCSGFGCCQLQIPKVLK 188
           +TKNKFTVIGCDTYA++ GQ+EG+ +RTACVA CD+I+ V+DG+CSG GCCQL IP  LK
Sbjct: 832 STKNKFTVIGCDTYAFLSGQIEGQSYRTACVALCDNITTVRDGACSGNGCCQLDIPSGLK 891

Query: 189 DLKYEVYSFKNHSEVQDFNPCGYAFLIEEDMFNFSKTFIRDF 230
            L+Y V SF NH++V  FNPCGYAF+ EED F+FS  +IRDF
Sbjct: 892 GLRYRVRSFDNHTDVLSFNPCGYAFVTEEDKFHFSAAYIRDF 932

BLAST of Spg008623.1 vs. ExPASy TrEMBL
Match: A0A6J1CJM0 (putative wall-associated receptor kinase-like 16 OS=Momordica charantia OX=3673 GN=LOC111011910 PE=4 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 5.0e-67
Identity = 136/233 (58.37%), Postives = 168/233 (72.10%), Query Frame = 0

Query: 5   MEALVRRLV-VLKISILAVAA--AALAASQAKPGCDDMCGNLTIPYPFGMSKDCYLDEAF 64
           ME L+R ++  ++I IL+ AA  AA  ASQA PGC++ CG++ IPYPFGM + CYL+E F
Sbjct: 1   METLMRLIITAVQIGILSAAAATAAAGASQALPGCEEWCGDVQIPYPFGMKEGCYLNEKF 60

Query: 65  FILCNKTHYDPPMAFLTGGNIEITNISILHGELHILHYVARDCYAQDGRNIKNDKPTLSL 124
           FI CNKTH D P AFL GGN+ +TNISI  GEL+ILH+ ARDCY        N  P LS 
Sbjct: 61  FISCNKTHNDSPKAFLQGGNLNVTNISI-SGELNILHFWARDCYP------PNTLPDLST 120

Query: 125 A----NFTISNTKNKFTVIGCDTYAYIYGQLEGEKF-RTACVASCDDISQVKDGSCSGFG 184
           A     F +S+ KNKFTVIGCDT+++I G + G +F ++ACVA CDDI+ VKDG+CSG G
Sbjct: 121 AFLNTEFAVSSAKNKFTVIGCDTFSFISGLVGGTQFYKSACVALCDDITTVKDGACSGNG 180

Query: 185 CCQLQIPKVLKDLKYEVYSFKNHSEVQDFNPCGYAFLIEEDMFNFSKTFIRDF 230
           CCQL+IPK L +L Y V SF NH+ V  FNPCGYAF+IEED FNFS  +IR F
Sbjct: 181 CCQLEIPKGLNNLNYTVSSFDNHTNVLSFNPCGYAFVIEEDKFNFSSKYIRHF 226

BLAST of Spg008623.1 vs. TAIR 10
Match: AT1G21270.1 (wall-associated kinase 2 )

HSP 1 Score: 148.7 bits (374), Expect = 5.9e-36
Identity = 79/208 (37.98%), Postives = 120/208 (57.69%), Query Frame = 0

Query: 17  ISILAVAAAALAASQAKPGCDDMCGNLTIPYPFGMSKDCYL--DEAFFILCNKTHYDPPM 76
           +++  +A   L   Q +  C   CGN+ + YPFG S  CY   DE+F + CN+       
Sbjct: 10  VAVFYLAYTQLVKGQPRKECQTRCGNVAVEYPFGTSPGCYYPGDESFNLTCNEQEK---- 69

Query: 77  AFLTGGNIEITNISILHGELHILHYVARDCYAQDGRNIKNDKPTLSLANFTISNTKNKFT 136
             L  GN+ + N+S L G+L +    +R CY   G+         +L NFT+S   N+FT
Sbjct: 70  --LFFGNMPVINMS-LSGQLRVRLVRSRVCYDSQGKQTDYIAQRTTLGNFTLSEL-NRFT 129

Query: 137 VIGCDTYAYIYGQLEG-EKFRTACVASCDDISQVKDGSCSGFGCCQLQIPKVLKDLKYEV 196
           V+GC++YA++  +  G EK+ T C++ CD  +  K+GSCSG GCCQ+ +P+    ++ + 
Sbjct: 130 VVGCNSYAFL--RTSGVEKYSTGCISICDS-ATTKNGSCSGEGCCQIPVPRGYSFVRVKP 189

Query: 197 YSFKNHSEVQDFNPCGYAFLIEEDMFNF 222
           +SF NH  V  FNPC YAFL+E+ MF+F
Sbjct: 190 HSFHNHPTVHLFNPCTYAFLVEDGMFDF 206

BLAST of Spg008623.1 vs. TAIR 10
Match: AT1G21250.1 (cell wall-associated kinase )

HSP 1 Score: 126.3 bits (316), Expect = 3.1e-29
Identity = 74/212 (34.91%), Postives = 116/212 (54.72%), Query Frame = 0

Query: 21  AVAAAALAASQAKPG--CDDMCGNLTIPYPFGMSKDCYL--DEAFFILCNKTHYDPPMAF 80
           ++A   L   Q +PG  C + CGN+TI YPFG+S  CY   +E+F I C +   D P   
Sbjct: 15  SLACTQLVKGQHQPGENCQNKCGNITIEYPFGISSGCYYPGNESFSITCKE---DRPHVL 74

Query: 81  LTGGNIEITNISILHGELHILHYVARDCYAQDGRNIKNDKPTLSLANFTISNTKNKFTVI 140
               +IE+ N +   G+L +L   +  CY + G+  + D  + +L N ++S   NK T +
Sbjct: 75  ---SDIEVANFN-HSGQLQVLLNRSSTCYDEQGKKTEEDS-SFTLENLSLS-ANNKLTAV 134

Query: 141 GCDTYAYI--YGQLEGEKFRTACVASCDDISQVKDGSCSGFGCCQLQIPKVLKDLKYEVY 200
           GC+  + +  +G    + + TAC++ CD   +  DG C+G GCC++ +   L    +E  
Sbjct: 135 GCNALSLLDTFGM---QNYSTACLSLCDSPPEA-DGECNGRGCCRVDVSAPLDSYTFETT 194

Query: 201 S--FKNHSEVQDFNPCGYAFLIEEDMFNFSKT 225
           S   K+ +   DF+PC YAFL+E+D FNFS T
Sbjct: 195 SGRIKHMTSFHDFSPCTYAFLVEDDKFNFSST 213

BLAST of Spg008623.1 vs. TAIR 10
Match: AT1G21230.1 (wall associated kinase 5 )

HSP 1 Score: 106.7 bits (265), Expect = 2.6e-23
Identity = 68/205 (33.17%), Postives = 103/205 (50.24%), Query Frame = 0

Query: 22  VAAAALAASQAKPGCDDMCGNLTIPYPFGMSKDCYL--DEAFFILCNKTHYDPPMAFLTG 81
           +A   L  +Q +  C   CG++ I YPFG+S  CY   D++F I C +   D P      
Sbjct: 15  LAYTQLVKAQPRDDCQTRCGDVPIDYPFGISTGCYYPGDDSFNITCEE---DKPNVL--- 74

Query: 82  GNIEITNISILHGELHILHYVARDCYAQDGRNIKNDKPTLSLANFTISNTKNKFTVIGCD 141
            NIE+ N +   G+L  L   +  CY Q   N   +     L N + S   NKFT++GC+
Sbjct: 75  SNIEVLNFN-HSGQLRGLIPRSTVCYDQQTNN-DFESLWFRLDNLSFS-PNNKFTLVGCN 134

Query: 142 TYAYIYGQLEGEKFRTACVASCDDISQVKDGSCSGFGCCQLQIPKVLKDLKYEVY--SFK 201
            +A +      + + T C++ C D     +  C+G GCC+ ++   L   + E     F+
Sbjct: 135 AWA-LLSTFGIQNYSTGCMSLC-DTPPPPNSKCNGVGCCRTEVSIPLDSHRIETQPSRFE 194

Query: 202 NHSEVQDFNPCGYAFLIEEDMFNFS 223
           N + V+ FNPC YAF +E+ MFNFS
Sbjct: 195 NMTSVEHFNPCSYAFFVEDGMFNFS 208

BLAST of Spg008623.1 vs. TAIR 10
Match: AT1G21210.1 (wall associated kinase 4 )

HSP 1 Score: 104.0 bits (258), Expect = 1.7e-22
Identity = 67/218 (30.73%), Postives = 107/218 (49.08%), Query Frame = 0

Query: 9   VRRLVVLKISILAVAAAALAASQAKPGCDDMCGNLTIPYPFGMSKDCYL--DEAFFILCN 68
           V+RL +  ++I  ++   L   Q  P C + CGN+T+ YPFG S  C+   D +F + C 
Sbjct: 3   VQRLFL--VAIFCLSYMQLVKGQTLPRCPEKCGNVTLEYPFGFSPGCWRAEDPSFNLSCV 62

Query: 69  KTHYDPPMAFLTGGNIEITNISILHGELHILHYVARDCYAQDGRNIKNDKPTLSLANFTI 128
             +      F  G  +E+  IS    +L +L+  +  CY   G+  K      +L N T+
Sbjct: 63  NEN-----LFYKG--LEVVEIS-HSSQLRVLYPASYICYNSKGKFAKGTYYWSNLGNLTL 122

Query: 129 SNTKNKFTVIGCDTYAYIYGQLEGEKFRTACVASCDDISQVKDGSCSGFGCCQLQIPKVL 188
           S   N  T +GC++YA++       +    C+++CD +S   +G C+G GCCQ  +P   
Sbjct: 123 SG-NNTITALGCNSYAFVSSN-GTRRNSVGCISACDALSHEANGECNGEGCCQNPVPAGN 182

Query: 189 KDLKYEVYSFKNHSEVQDFN--PCGYAFLIEEDMFNFS 223
             L    Y F N + VQ  +   C YAFL+E   F ++
Sbjct: 183 NWLIVRSYRFDNDTSVQPISEGQCIYAFLVENGKFKYN 208

BLAST of Spg008623.1 vs. TAIR 10
Match: AT1G21240.1 (wall associated kinase 3 )

HSP 1 Score: 94.7 bits (234), Expect = 1.0e-19
Identity = 67/227 (29.52%), Postives = 106/227 (46.70%), Query Frame = 0

Query: 17  ISILAVAAAALAASQAKPGCDDMCGNLTIPYPFGMSKDCYL--DEAFFILCNKTHYDPPM 76
           I  LA         Q +  C   CGN+TI YPFG+S  CY   D+ F + C         
Sbjct: 12  IFFLAYTQLVKGQHQPREDCKLKCGNVTIEYPFGISTGCYYPGDDNFNLTCVVEE----- 71

Query: 77  AFLTGGNIEITNISILHGELHILHYVARDCYAQDGRNIKNDKPTLSL-----ANFTISNT 136
             L  G I++TNIS   G + +L     +CY Q     KN+    +L     ++F++S +
Sbjct: 72  KLLLFGIIQVTNIS-HSGHVSVLFERFSECYEQ-----KNETNGTALGYQLGSSFSLS-S 131

Query: 137 KNKFTVIGCDTYAYIYGQLEGEKFRTACVASCDDISQVKDGSCSGFGCC---QLQIPKVL 196
            NKFT++GC+  + +      + + T C++ C+   +  +G C+G GCC      +P   
Sbjct: 132 NNKFTLVGCNALS-LLSTFGKQNYSTGCLSLCNSQPEA-NGRCNGVGCCTTEDFSVPFDS 191

Query: 197 KDLKYEVYSFKN---------HSEVQDFNPCGYAFLIEEDMFNFSKT 225
              ++     +N         ++ V  FNPC YAFL+E+  FNF  +
Sbjct: 192 DTFQFGSVRLRNQVNNSLDLFNTSVYQFNPCTYAFLVEDGKFNFDSS 224