PI0016550.2 (mRNA) Melon (PI 482460) v1

Overview


Name	PI0016550.2
Type	mRNA
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Guanylate kinase
Location	chr01: 494442 .. 497887 (+)
Sequence length	2302
RNA-Seq Expression	PI0016550.2
Synteny	PI0016550.2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCGTCCCGCCCGGCCATCTCTAGTTGTGATACTGTAAACTGCACTTCCCATTTTTCTTCTCCCTCATGGCTCGCAGATTCTTCCGCACTTCTCTTTCCCTTTCCCGCTCTTTAATGCTCCACACTGAATTTCACAATCCCATTCCTTTTCCTAATTTACTCAAACCCATTCAATCATTCCCTCTAACCCGACCATTTTTCTCCTCCATTTCTCAAATCGACGATGCCCTTTCCCCCACCATTCTGCAGCCCGAAAAAGCCAACCGATTGGAATTGCTTCGCTCCCTTGAATTTTTGTTCGGTTCTTCGTTTCGCTCCGACCCTTTGGTCCCCAATCCAAGCCCTTTAGTTATTGTGATCAGTGGCCCACCTGGGGTCGGAAATGATGCTGTGATTAAACGATTGAGAGAAGTCCGAGAAGGGCTCCATTTCGCTGTCACAGCGACGACTCGTCCCATGCGACCTGGTGAAGTCGATGGAAAAGATTGTTATTTCGTCTCCAAAGAAGAGTTTCTTGCTATGATGAAGAGGAACGAGCTTCTGGAATATACGTCAACGTATGGAAACTACAAGGGGACTCCCAAAACGCAAATTCGTGAGTCTATGGAAAAAGGGTATGATATTGTGTTGACAGTTGAAATTCCAAGAGCTAAAATATTGAGGAAGATTCTTCGAGATTCTGCTGTTTTTGTGTTTTTGAAGGCGGAGAGTAAGGTTAAATTAGGGGAGAGGTTGATTGATAGGAAGGCGGGGACTGAAGAATCATTGTTGGTGAGACTTCCATTGGCTAGAGAGGAAGTGAAGCATGTTAAGAATTTTGATTACGTGGTTGTGAATGCAGATGGGAAGTTGGAGAGTGCAGTTAAGCTTGTGGAATCCATTATTGATGCTGAGAAGGCAAAGGTCTGGCAGAGAAAGACTGTTGTTAGATTCAGCTTTTGAGACATCATTTTGCAAGGTAACTTTTGTTTTATAGTTCTATTGGGAGATGGGTTTCTTTCTTAGAAAAGTTTTACTGTTAGTGATTGAGAAGGAACAACAGAATCATACCTTACCTACTCTGTTTGAAACTCCAGCTTCCTCTATTCAAGTATTTATCGCTTTTATTTGTTTGATAGTAGAATAGTTTTGCCTGAAACTTGCATAGTTTTGTTTTGGATTCTATTTCAAATGCCTGGTAATGGTATCACTGGAGAAGTTTTCATTATTCATGTATCCAAGTTGTTACTGACTTCTAACAGGCTACATCTCAACATTTAGCTTAATAGTATTGAGTCTTATTGTATAAAAAACCAACTTTTGATTTCTTGGAGATTTCATCAGCCATAATGGTCGGCTTTTTTATACTCTCTTTGGCGATATTCTTCATAAAGTCTAATGGTTTCTCGTGATTGTCATATCACGCTTCGATGGAGAACATTATGTTATTAGATGGATGAATTTTCAGGTTGCTTCTTATTAAAGGATACTCTCCCTCCTCTTTTAAGATTAGAATTCAGATTGATCTGAGAGATCTAACTGGTGTGATATAGAAGGACAACTCTCCCTAGAATCGAAACTCAAATCTTACTGTTAGTCGAAGGTTACTGAATACATGAGATGAGAGATAGAGAACCAAAAACAAATTAAGAACTAAAATCCAAACAGGAAATTAAAAAGGATTAGCCAGGGAATTACTCAATTACCCAAGGCTAGGATAACTACTATATTACATCATAACAGGAGGTCTATTGATATGCAAATGGAAGGAAACGATCATCGAGTATGATTGAACCTAGAATCTCTAATGGAGTGTAAGTGGGTCGGTTAATCTTATTTTTTGGTCAAAACTGGCTTTATACTAACCTACTGATACAAGAAAAATGTGAAAATCAACATTCAAACCAACATTCTAGATAACCAATGCCAACCGAATAGACTATACCAGCCCCTAGATTACTTTTTGTATTTGGAGTTTCATGAAACTGAACCAACGGACATGTATTGCTCAAATCAATTGACTAGCTCTCCTACTTTAAGCTTTAATGCTATGTTCTGGTTATCATTGGACTAGAAAATTTGGAGTGCTAATCTTCTTTTTTTTTTTTTTTTTTTTGAAACAGAAACTAAACTTTTCATCGATGAAATGAAATGAGGCTAATGCTCAAAATATAATGACAGAAAAGAGCCAAAATACAAACATAGAAATTTGGGATCAGCAGGTGTTGCAAGATATCTCAACTAGGTTGGCACAGCTATAAACATCCTCATCACATCCCTAAACAAGAGGACTCATGTACATTATCTTTGTAACCTGATTACGTAGAAAGTAAAAGCATTCCAATTAAGAAAAATATCCTTAATAGGATAATTTTCAAAGAACTTCGACATGGGAACTCCACGAGGATGCATGCTAGTGAATAAGTAGAACCAAGGCGATCTGACCAATCCAAGGGCTTATCATCAAAGACTCTAATTTATTTCAAACCAAATCTCAGAAATTAAGGCGTTAACTGCATTTGACCAAAGAATCTGTGCTTTAGAAAAAGCAACGGGCCAACCAATATTGGGACATAGTTTCTCGAAGTGACTGCTCAAAAACCAAGATAAGTTGAAGCAGAAGAACGACTTCCACCAGCGCTTGCTTGTAAAGGAGCGAACAAACAAAAGATGCTGTAAATTTTCTCCGTCCTCTAGACACAACAGGCAAATAGAAGGAGATGAGCTTTGATTCCTAAGCTAATGTTGTAAAGTGGCTGAGCAATTTAGAGACCCAAACAACAGAATCCATATCAACACATTGACTCTTTTGGGACACTTCGATCTCCAAAGGCCACAATAAGTATCTTTTTGTAAATGGGAAGAAGAGTTTAAATATTTTGTCAAAGATTTTACCGAGAACCTTTTTGAGGAATCCAAAGACCATACCCGAGCATCATAAGCATGAGAAATTCTACTATTTTCTACTTCACACAGTAGCTACTGGAAATCTGTTATTTGACTCCTCTTTTACTGCCTACGGAAGATTAGATTCAGTGGTTGTATCCCAATGATCATTGGTGAGGAGGCAATGCAAAACAATACAGGGAAACTCACCTTAGAGGAGGTTTTCGAACACCATGAATCAGACCAAAAGTAAATTTGATGCTTGTACTCCAAGGCTTTTGGAAATGGCTGAGTTGTACTCCTTTTCTGCTTGGCTCTTATTTCTCCCCTTTAACTGCACACAAAATAACTCTATTTCCCCACCATTTCCTTCTTCAAGATTTTAGGTCTAACTGATTTATTCACTTATTTATAAACACAAAAAATGAGACACGTCAGAGGGGTTTGGTATAGAAAGATGCAAAGGTTTTTGTTATTCATAATTTAAATTCTATTTTGGTCTATAAAATATTAAAAACCCTTCAAATAATATGATCGTGTATTTAGAAACAATAATGGCTATCGTTTTGCCTCCTTCACTT

mRNA sequence

CCCGTCCCGCCCGGCCATCTCTAGTTGTGATACTGTAAACTGCACTTCCCATTTTTCTTCTCCCTCATGGCTCGCAGATTCTTCCGCACTTCTCTTTCCCTTTCCCGCTCTTTAATGCTCCACACTGAATTTCACAATCCCATTCCTTTTCCTAATTTACTCAAACCCATTCAATCATTCCCTCTAACCCGACCATTTTTCTCCTCCATTTCTCAAATCGACGATGCCCTTTCCCCCACCATTCTGCAGCCCGAAAAAGCCAACCGATTGGAATTGCTTCGCTCCCTTGAATTTTTGTTCGGTTCTTCGTTTCGCTCCGACCCTTTGGTCCCCAATCCAAGCCCTTTAGTTATTGTGATCAGTGGCCCACCTGGGGTCGGAAATGATGCTGTGATTAAACGATTGAGAGAAGTCCGAGAAGGGCTCCATTTCGCTGTCACAGCGACGACTCGTCCCATGCGACCTGGTGAAGTCGATGGAAAAGATTGTTATTTCGTCTCCAAAGAAGAGTTTCTTGCTATGATGAAGAGGAACGAGCTTCTGGAATATACGTCAACGTATGGAAACTACAAGGGGACTCCCAAAACGCAAATTCGTGAGTCTATGGAAAAAGGGTATGATATTGTGTTGACAGTTGAAATTCCAAGAGCTAAAATATTGAGGAAGATTCTTCGAGATTCTGCTGTTTTTGTGTTTTTGAAGGCGGAGAGTAAGGTTAAATTAGGGGAGAGGTTGATTGATAGGAAGGCGGGGACTGAAGAATCATTGTTGGTGAGACTTCCATTGGCTAGAGAGGAAGTGAAGCATGTTAAGAATTTTGATTACGTGGTTGTGAATGCAGATGGGAAGTTGGAGAGTGCAGTTAAGCTTGTGGAATCCATTATTGATGCTGAGAAGGCAAAGGTCTGGCAGAGAAAGACTGTTGTTAGATTCAGCTTTTGAGACATCATTTTGCAAGAAACTAAACTTTTCATCGATGAAATGAAATGAGGCTAATGCTCAAAATATAATGACAGAAAAGAGCCAAAATACAAACATAGAAATTTGGGATCAGCAGGTGTTGCAAGATATCTCAACTAGGTTGGCACAGCTATAAACATCCTCATCACATCCCTAAACAAGAGGACTCATGTACATTATCTTTGTAACCTGATTACGTAGAAAGTAAAAGCATTCCAATTAAGAAAAATATCCTTAATAGGATAATTTTCAAAGAACTTCGACATGGGAACTCCACGAGGATGCATGCTAGTGAATAAGTAGAACCAAGGCGATCTGACCAATCCAAGGGCTTATCATCAAAGACTCTAATTTATTTCAAACCAAATCTCAGAAATTAAGGCGTTAACTGCATTTGACCAAAGAATCTGTGCTTTAGAAAAAGCAACGGGCCAACCAATATTGGGACATAGTTTCTCGAAGTGACTGCTCAAAAACCAAGATAAGTTGAAGCAGAAGAACGACTTCCACCAGCGCTTGCTTGTAAAGGAGCGAACAAACAAAAGATGCTGTAAATTTTCTCCGTCCTCTAGACACAACAGGCAAATAGAAGGAGATGAGCTTTGATTCCTAAGCTAATGTTGTAAAGTGGCTGAGCAATTTAGAGACCCAAACAACAGAATCCATATCAACACATTGACTCTTTTGGGACACTTCGATCTCCAAAGGCCACAATAAGTATCTTTTTGTAAATGGGAAGAAGAGTTTAAATATTTTGTCAAAGATTTTACCGAGAACCTTTTTGAGGAATCCAAAGACCATACCCGAGCATCATAAGCATGAGAAATTCTACTATTTTCTACTTCACACAGTAGCTACTGGAAATCTGTTATTTGACTCCTCTTTTACTGCCTACGGAAGATTAGATTCAGTGGTTGTATCCCAATGATCATTGGTGAGGAGGCAATGCAAAACAATACAGGGAAACTCACCTTAGAGGAGGTTTTCGAACACCATGAATCAGACCAAAAGTAAATTTGATGCTTGTACTCCAAGGCTTTTGGAAATGGCTGAGTTGTACTCCTTTTCTGCTTGGCTCTTATTTCTCCCCTTTAACTGCACACAAAATAACTCTATTTCCCCACCATTTCCTTCTTCAAGATTTTAGGTCTAACTGATTTATTCACTTATTTATAAACACAAAAAATGAGACACGTCAGAGGGGTTTGGTATAGAAAGATGCAAAGGTTTTTGTTATTCATAATTTAAATTCTATTTTGGTCTATAAAATATTAAAAACCCTTCAAATAATATGATCGTGTATTTAGAAACAATAATGGCTATCGTTTTGCCTCCTTCACTT

Coding sequence (CDS)

ATGGCTCGCAGATTCTTCCGCACTTCTCTTTCCCTTTCCCGCTCTTTAATGCTCCACACTGAATTTCACAATCCCATTCCTTTTCCTAATTTACTCAAACCCATTCAATCATTCCCTCTAACCCGACCATTTTTCTCCTCCATTTCTCAAATCGACGATGCCCTTTCCCCCACCATTCTGCAGCCCGAAAAAGCCAACCGATTGGAATTGCTTCGCTCCCTTGAATTTTTGTTCGGTTCTTCGTTTCGCTCCGACCCTTTGGTCCCCAATCCAAGCCCTTTAGTTATTGTGATCAGTGGCCCACCTGGGGTCGGAAATGATGCTGTGATTAAACGATTGAGAGAAGTCCGAGAAGGGCTCCATTTCGCTGTCACAGCGACGACTCGTCCCATGCGACCTGGTGAAGTCGATGGAAAAGATTGTTATTTCGTCTCCAAAGAAGAGTTTCTTGCTATGATGAAGAGGAACGAGCTTCTGGAATATACGTCAACGTATGGAAACTACAAGGGGACTCCCAAAACGCAAATTCGTGAGTCTATGGAAAAAGGGTATGATATTGTGTTGACAGTTGAAATTCCAAGAGCTAAAATATTGAGGAAGATTCTTCGAGATTCTGCTGTTTTTGTGTTTTTGAAGGCGGAGAGTAAGGTTAAATTAGGGGAGAGGTTGATTGATAGGAAGGCGGGGACTGAAGAATCATTGTTGGTGAGACTTCCATTGGCTAGAGAGGAAGTGAAGCATGTTAAGAATTTTGATTACGTGGTTGTGAATGCAGATGGGAAGTTGGAGAGTGCAGTTAAGCTTGTGGAATCCATTATTGATGCTGAGAAGGCAAAGGTCTGGCAGAGAAAGACTGTTGTTAGATTCAGCTTTTGA

Protein sequence

MARRFFRTSLSLSRSLMLHTEFHNPIPFPNLLKPIQSFPLTRPFFSSISQIDDALSPTILQPEKANRLELLRSLEFLFGSSFRSDPLVPNPSPLVIVISGPPGVGNDAVIKRLREVREGLHFAVTATTRPMRPGEVDGKDCYFVSKEEFLAMMKRNELLEYTSTYGNYKGTPKTQIRESMEKGYDIVLTVEIPRAKILRKILRDSAVFVFLKAESKVKLGERLIDRKAGTEESLLVRLPLAREEVKHVKNFDYVVVNADGKLESAVKLVESIIDAEKAKVWQRKTVVRFSF

Homology

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BLAST of PI0016550.2 vs. ExPASy Swiss-Prot
Match: Q94JM2 (Guanylate kinase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=GK3 PE=2 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 8.4e-77
Identity = 160/276 (57.97%), Postives = 200/276 (72.46%), Query Frame = 0


Query: 18  LHTEFHNPIPFPNLLKPI---QSFP-----LTRPFFSSISQIDDAL--SPTILQPEKANR 77
           L + F   I FPN  KPI   +SFP      +   +SS+   D  +  +P     +   +
Sbjct: 5   LCSSFSRSIIFPN--KPIFIPRSFPQPLRHSSALSYSSLKMTDTHIPGNPNSSPIQSPTK 64

Query: 78  LELLRSLEFLFGSSFRSDPLVPNPSPLVIVISGPPGVGNDAVIKRLREVREGLHFAVTAT 137
            E LRSLEF  GSSF +DP++P P+ +VIVISGP GVG DAVI +LREVREGLHF VTAT
Sbjct: 65  PESLRSLEFQLGSSFTADPIIPPPNQIVIVISGPSGVGKDAVINKLREVREGLHFVVTAT 124

Query: 138 TRPMRPGEVDGKDCYFVSKEEFLAMMKRNELLEYTSTYGNYKGTPKTQIRESMEKGYDIV 197
           +RPMRPGEVDGKD +FVS+++FL+M++  ELLEY   YG YKG PK QI+E M KG DIV
Sbjct: 125 SRPMRPGEVDGKDYFFVSRDQFLSMVENEELLEYALVYGEYKGIPKKQIQEFMAKGEDIV 184

Query: 198 LTVEIPRAKILRKILRDSAVFVFLKAESKVKLGERLIDRKAGTEESLLVRLPLAREEVKH 257
           L V+I  A+ LR+IL +SAVF+FL AES++ + ERLIDRK  ++E LLVR+  AREEV+H
Sbjct: 185 LRVDIQGAQTLRRILGNSAVFIFLVAESELAMVERLIDRKTESQEELLVRVATAREEVRH 244

Query: 258 VKNFDYVVVNADGKLESAVKLVESIIDAEKAKVWQR 284
           +KNFDYVVVNA G+L+ AV  VESIIDAEK+KV QR
Sbjct: 245 LKNFDYVVVNAKGRLDDAVNRVESIIDAEKSKVHQR 278

[Close]

BLAST of PI0016550.2 vs. ExPASy Swiss-Prot
Match: Q10M74 (Guanylate kinase 2, chloroplastic/mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=V2 PE=1 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 4.3e-73
Identity = 157/283 (55.48%), Postives = 192/283 (67.84%), Query Frame = 0


Query: 1   MARRFFRTSLSLSRSLMLHTEFHNPIPFPNLLKPIQSFPLTRPFFSSISQIDDALSPTIL 60
           + RRF   S +L+RS +L      P   P    P    P  R   SS S    +  P   
Sbjct: 3   LTRRF---SSALARSPLLPRSLPPPRAVP-ATPPAPRPPPRRLMSSSSSGWHHSSRPPPP 62

Query: 61  QPEKANRLELLRSLEFLFGSSFRSDPLVPNPSPLVIVISGPPGVGNDAVIKRLREVREGL 120
            P  A++ +L R LE   G++F S+PL P P P+++VISGP GVG DAVI+RL+E REG+
Sbjct: 63  PPSGADKDQLFRGLEAALGTTFSSEPLAPPPQPMILVISGPSGVGKDAVIQRLQEEREGM 122

Query: 121 HFAVTATTRPMRPGEVDGKDCYFVSKEEFLAMMKRNELLEYTSTYGNYKGTPKTQIRESM 180
           HF VTAT+R  RPGEVDGKD YFV+KEEFL M++R ELLEY   YG YKG PK QIR+ M
Sbjct: 123 HFVVTATSRAKRPGEVDGKDYYFVTKEEFLTMIERKELLEYALVYGEYKGIPKQQIRDYM 182

Query: 181 EKGYDIVLTVEIPRAKILRKILRDSAVFVFLKAESKVKLGERLIDRKAGTEESLLVRLPL 240
            KGYDIVL V+I  A  LR+IL +SA+F+FL AES+  L +RLI RK  T + LLVR+  
Sbjct: 183 AKGYDIVLRVDIQGAATLREILGESAIFIFLVAESEEALVKRLIHRKTETSDMLLVRVAT 242

Query: 241 AREEVKHVKNFDYVVVNADGKLESAVKLVESIIDAEKAKVWQR 284
           AREEVK + NFDYVVVN++G LE AVK VESIIDAEKAKV +R
Sbjct: 243 AREEVKRMNNFDYVVVNSEGNLEGAVKQVESIIDAEKAKVHKR 281

[Close]

BLAST of PI0016550.2 vs. ExPASy Swiss-Prot
Match: Q3ZWD8 (Guanylate kinase OS=Dehalococcoides mccartyi (strain CBDB1) OX=255470 GN=gmk PE=3 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 1.6e-35
Identity = 78/191 (40.84%), Postives = 124/191 (64.92%), Query Frame = 0


Query: 93  PLVIVISGPPGVGNDAVIKRLREVREGLHFAVTATTRPMRPGEVDGKDCYFVSKEEFLAM 152
           PL++++SGP GVG DAV+ R++E +  L + VT TTRP R  E +G D  F+   EF  +
Sbjct: 13  PLLLIVSGPSGVGKDAVLARMKERKLPLTYIVTTTTRPKRETETEGVDYNFIRPAEFQEL 72

Query: 153 MKRNELLEYTSTYGNYKGTPKTQIRESMEKGYDIVLTVEIPRAKILRKILRDSAVFVFLK 212
           + +NELLE+ + YGN+ G PK  IR+++ +G D+++ V++  A  ++KI+  +AVF+FL 
Sbjct: 73  ISQNELLEWANVYGNFYGVPKAPIRQALSRGSDVIVKVDVQGAASIKKIV-PNAVFIFLM 132

Query: 213 AESKVKLGERLIDRKAGTEESLLVRLPLAREEVKHVKNFDYVVVNADGKLESAVKLVESI 272
                +L  RL  R   + ESL  RL  A  E++ + +FDYVVVN +G++++AV+ + SI
Sbjct: 133 PPDMDELTRRLEHRLTESPESLRRRLATAPLEIEKLPDFDYVVVNPEGEIDNAVREIMSI 192

Query: 273 IDAEKAKVWQR 284
           I AE  ++  R
Sbjct: 193 IAAEHCRIQPR 202

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BLAST of PI0016550.2 vs. ExPASy Swiss-Prot
Match: Q3ZAF9 (Guanylate kinase OS=Dehalococcoides mccartyi (strain ATCC BAA-2266 / KCTC 15142 / 195) OX=243164 GN=gmk PE=3 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 3.0e-34
Identity = 77/188 (40.96%), Postives = 121/188 (64.36%), Query Frame = 0


Query: 93  PLVIVISGPPGVGNDAVIKRLREVREGLHFAVTATTRPMRPGEVDGKDCYFVSKEEFLAM 152
           PL++V+SGP GVG DAV+ R++E +  L + VT TTR  R  E +G D  F+   EF  +
Sbjct: 13  PLLLVVSGPSGVGKDAVLARMKERKLPLAYIVTTTTRTKREKETEGVDYNFIRPAEFQQL 72

Query: 153 MKRNELLEYTSTYGNYKGTPKTQIRESMEKGYDIVLTVEIPRAKILRKILRDSAVFVFLK 212
           + +NELLE+ + YGN+ G PK  IR+++  G+D+++ V++  A  ++KI+  +AVF+FL 
Sbjct: 73  IGQNELLEWANVYGNFYGVPKAPIRQALAHGFDVIVKVDVQGAASIKKIV-PNAVFIFLM 132

Query: 213 AESKVKLGERLIDRKAGTEESLLVRLPLAREEVKHVKNFDYVVVNADGKLESAVKLVESI 272
                +L  RL  R   + ESL  RL  A  E++ + +FDYVVVN +G++++AV  + SI
Sbjct: 133 PPDMDELTRRLEHRLTESPESLKRRLATAPLEIEKLPDFDYVVVNPEGEIDNAVSEIMSI 192

Query: 273 IDAEKAKV 281
           I AE  ++
Sbjct: 193 ISAEHCRI 199

[Close]

BLAST of PI0016550.2 vs. ExPASy Swiss-Prot
Match: Q895Q5 (Guanylate kinase OS=Clostridium tetani (strain Massachusetts / E88) OX=212717 GN=gmk PE=3 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 4.0e-34
Identity = 81/197 (41.12%), Postives = 121/197 (61.42%), Query Frame = 0


Query: 94  LVIVISGPPGVGNDAVIKRLREVREGLHFAVTATTRPMRPGEVDGKDCYFVSKEEFLAMM 153
           L+IVISGP G G   V K L        F+V++TTR  R GE+ GK  YF+SKEEF   +
Sbjct: 5   LLIVISGPSGTGKGTVCKELLR-NNNFWFSVSSTTRDPREGEIQGKSYYFMSKEEFEDKI 64

Query: 154 KRNELLEYTSTYGNYKGTPKTQIRESMEKGYDIVLTVEIPRAKILRKILRDSAVFVFLKA 213
           K N+ LEY   YGNY GTPK+++ E ++KG D++L ++I  A  +++  ++  +F+F+  
Sbjct: 65  KENDFLEYAKVYGNYYGTPKSKVIEMLDKGKDVILEIDIQGALQVKENYKE-GIFIFILP 124

Query: 214 ESKVKLGERLIDRKAGTEESLLVRLPLAREEVKHVKNFDYVVVNADGKLESAVKLVESII 273
            S  +L  R+I R   TEESL+ R   A +E+ +V  ++Y VVN   K+  AV+ ++SII
Sbjct: 125 PSMEELKNRIIKRGTETEESLMTRFKSAYKEINYVSKYNYAVVN--DKVHDAVEKIQSII 184

Query: 274 DAEKAKVWQRKTVVRFS 291
            AEK +V + K  +  S
Sbjct: 185 SAEKCRVDRIKDSILLS 197

[Close]

BLAST of PI0016550.2 vs. ExPASy TrEMBL
Match: A0A0A0K367 (Guanylate kinase-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G073640 PE=4 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 1.4e-122
Identity = 239/287 (83.28%), Postives = 253/287 (88.15%), Query Frame = 0


Query: 1   MARRFFRTSLSLSRSLMLHTEFHNPIPFPNLLKPIQSFPLTRPFFSSISQIDDALSPTIL 60
           MARRFF TSLSLSRSL  HT+F NPIPFPNL+KPIQSF LT PFFSS+SQI DALSPTI 
Sbjct: 1   MARRFFSTSLSLSRSLTRHTKFPNPIPFPNLIKPIQSFHLTPPFFSSVSQIGDALSPTIQ 60

Query: 61  QPEKANRLELLRSLEFLFGSSFRSDPLVPNPSPLVIVISGPPGVGNDAVIKRLREVREGL 120
            P++ NR ELLRSLE L GSSFRSD LV N SPLVIV+SGPPGVGNDAVIKRLREVREGL
Sbjct: 61  PPDEDNRSELLRSLEVLLGSSFRSDRLVYNQSPLVIVLSGPPGVGNDAVIKRLREVREGL 120

Query: 121 HFAVTATTRPMRPGEVDGKDCYFVSKEEFLAMMKRNELLEYTSTYGNYKGTPKTQIRESM 180
           HFAVTATTRPMRPGEVDGKDCYF+SKEEF AMMKRNELLE++  YG+YKG PKTQIRE M
Sbjct: 121 HFAVTATTRPMRPGEVDGKDCYFISKEEFSAMMKRNELLEFSFMYGHYKGIPKTQIREFM 180

Query: 181 EKGYDIVLTVEIPRAKILRKILRDSAVFVFLKAESKVKLGERLIDRKAGTEESLLVRLPL 240
           EKGYDIVL VEI RAKILRKILR+SAVFVFL AES+ KLGER IDRK GTEESLLVRLP 
Sbjct: 181 EKGYDIVLRVEISRAKILRKILRNSAVFVFLMAESEAKLGERSIDRKTGTEESLLVRLPW 240

Query: 241 AREEVKHVKNFDYVVVNADGKLESAVKLVESIIDAEKAKVWQRKTVV 288
            RE   +VKNFDYVVVN DGKLESAVKLVESIIDAEKAKV QR+TVV
Sbjct: 241 VRE---NVKNFDYVVVNTDGKLESAVKLVESIIDAEKAKVRQRRTVV 284

[Close]

BLAST of PI0016550.2 vs. ExPASy TrEMBL
Match: A0A1S3AWS3 (Guanylate kinase OS=Cucumis melo OX=3656 GN=LOC103483755 PE=3 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 7.8e-110
Identity = 224/297 (75.42%), Postives = 241/297 (81.14%), Query Frame = 0


Query: 1   MARRFFRTSLSLSRSLMLHTEFHNPIPFPNLLKP-------IQSFPLTRPFFSSISQIDD 60
           M+RRFF T  SLSRS MLHT+FHNPIPFPN L P       IQSFPLT  FFSSISQ+ D
Sbjct: 1   MSRRFFAT--SLSRSSMLHTKFHNPIPFPNFLNPISLKSKTIQSFPLTPRFFSSISQMGD 60

Query: 61  ALS---PTILQPEKANRLELLRSLEFLFGSSFRSDPLVPNPSPLVIVISGPPGVGNDAVI 120
           A     PTI  P+KA+R ELLRSLEF  GSSF SDPLVPNPSPLVIVISGP GVG DAVI
Sbjct: 61  ARRPPFPTIPPPDKADRSELLRSLEFSLGSSFSSDPLVPNPSPLVIVISGPSGVGKDAVI 120

Query: 121 KRLREVREGLHFAVTATTRPMRPGEVDGKDCYFVSKEEFLAMMKRNELLEYTSTYGNYKG 180
           KRLREVREGLHF VTAT+RPMRPGEVDGKD YFVSKE+FL M+ RNELLEY   YG+YKG
Sbjct: 121 KRLREVREGLHFVVTATSRPMRPGEVDGKDYYFVSKEDFLDMIDRNELLEYALVYGDYKG 180

Query: 181 TPKTQIRESMEKGYDIVLTVEIPRAKILRKILRDSAVFVFLKAESKVKLGERLIDRKAGT 240
            PK QIRE M KGYDIVL V+   A  LRK+L +SAVFVFL AES+VKL ERLIDRK  T
Sbjct: 181 IPKRQIREFMAKGYDIVLRVDTQGAATLRKVLGNSAVFVFLMAESEVKLVERLIDRKTET 240

Query: 241 EESLLVRLPLAREEVKHVKNFDYVVVNADGKLESAVKLVESIIDAEKAKVWQRKTVV 288
           +ESLL+R+  AREEVKHV+NFDYVVVNADGKLESAVKLVESIIDAEKAKV QR  VV
Sbjct: 241 KESLLLRVATAREEVKHVRNFDYVVVNADGKLESAVKLVESIIDAEKAKVSQRNAVV 295

[Close]

BLAST of PI0016550.2 vs. ExPASy TrEMBL
Match: A0A6J1INA9 (Guanylate kinase OS=Cucurbita maxima OX=3661 GN=LOC111479039 PE=3 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 3.8e-104
Identity = 211/292 (72.26%), Postives = 238/292 (81.51%), Query Frame = 0


Query: 1   MARRFFRTSLSLSRSLMLHTEFHNPIPFPNL--LKPIQSFPLTRPFFSSISQIDDA---L 60
           M+ RFF    SLSRS  LH +FHNP   PN    KPIQSFPLT   FSSIS++ DA   +
Sbjct: 1   MSSRFF--GASLSRSSTLHPKFHNPNSLPNFPKSKPIQSFPLTPLSFSSISEMGDARRPI 60

Query: 61  SPTILQPEKANRLELLRSLEFLFGSSFRSDPLVPNPSPLVIVISGPPGVGNDAVIKRLRE 120
           S TI  P+KA+R ELLRSLEF  GSSF S+PLVPNPSPL+IVISGP GVG DAVIKRLRE
Sbjct: 61  SATIPPPDKADRSELLRSLEFSLGSSFSSEPLVPNPSPLIIVISGPSGVGKDAVIKRLRE 120

Query: 121 VREGLHFAVTATTRPMRPGEVDGKDCYFVSKEEFLAMMKRNELLEYTSTYGNYKGTPKTQ 180
           VREGLHF VTAT+RP+RPGEVDGKD YFVSKEEFLA+++RNELLEY   YG+YKG PK Q
Sbjct: 121 VREGLHFVVTATSRPIRPGEVDGKDYYFVSKEEFLALIERNELLEYALVYGDYKGIPKRQ 180

Query: 181 IRESMEKGYDIVLTVEIPRAKILRKILRDSAVFVFLKAESKVKLGERLIDRKAGTEESLL 240
           IR+ M KGYDIVL V+I  A+ LRKIL +SAVFVFL AE+++KL ERLI+RK  T+ESL+
Sbjct: 181 IRDFMAKGYDIVLRVDIQGAETLRKILGNSAVFVFLMAENELKLVERLINRKTETKESLM 240

Query: 241 VRLPLAREEVKHVKNFDYVVVNADGKLESAVKLVESIIDAEKAKVWQRKTVV 288
           VR+  AREEVKHV NFDYVVVNA+GKLESAVKLVESIIDAEKAKVWQR  VV
Sbjct: 241 VRVATAREEVKHVNNFDYVVVNAEGKLESAVKLVESIIDAEKAKVWQRNAVV 290

[Close]

BLAST of PI0016550.2 vs. ExPASy TrEMBL
Match: A0A6J1GV20 (Guanylate kinase OS=Cucurbita moschata OX=3662 GN=LOC111457439 PE=3 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 6.4e-104
Identity = 211/292 (72.26%), Postives = 236/292 (80.82%), Query Frame = 0


Query: 1   MARRFFRTSLSLSRSLMLHTEFHNPIPFPNL--LKPIQSFPLTRPFFSSISQIDDA---L 60
           M+ RFF    SLSRS  LH +FHNP   PN    KPIQSFPLT   FSSIS + DA   +
Sbjct: 1   MSSRFF--GASLSRSSTLHPKFHNPNSLPNFPKSKPIQSFPLTPRLFSSISGMGDARRPI 60

Query: 61  SPTILQPEKANRLELLRSLEFLFGSSFRSDPLVPNPSPLVIVISGPPGVGNDAVIKRLRE 120
           S TI  P+KA+R ELLRSLEF  GSSF S+PLVPNPSPL+IVISGP GVG DAVIKRLRE
Sbjct: 61  SDTIPAPDKADRSELLRSLEFSLGSSFSSEPLVPNPSPLIIVISGPSGVGKDAVIKRLRE 120

Query: 121 VREGLHFAVTATTRPMRPGEVDGKDCYFVSKEEFLAMMKRNELLEYTSTYGNYKGTPKTQ 180
           VREGLHF VTAT+RP+RPGEVDGKD YFVSKEEFLA+++RNELLEY   YG+YKG PK Q
Sbjct: 121 VREGLHFVVTATSRPIRPGEVDGKDYYFVSKEEFLALIERNELLEYALVYGDYKGIPKRQ 180

Query: 181 IRESMEKGYDIVLTVEIPRAKILRKILRDSAVFVFLKAESKVKLGERLIDRKAGTEESLL 240
           IR+ M KGYDIVL V+I  A+ LRKIL +SAVFVFL AE ++KL ERLI+RK  T+ESL+
Sbjct: 181 IRDFMAKGYDIVLRVDIQGAETLRKILGNSAVFVFLMAEDELKLVERLINRKTETKESLM 240

Query: 241 VRLPLAREEVKHVKNFDYVVVNADGKLESAVKLVESIIDAEKAKVWQRKTVV 288
           VR+  AREEVKHV NFDYVVVNA+GKLESAVKLVESIIDAEKAKVWQR  VV
Sbjct: 241 VRVATAREEVKHVNNFDYVVVNAEGKLESAVKLVESIIDAEKAKVWQRNAVV 290

[Close]

BLAST of PI0016550.2 vs. ExPASy TrEMBL
Match: A0A6J1C9N3 (Guanylate kinase OS=Momordica charantia OX=3673 GN=LOC111009480 PE=3 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 4.3e-100
Identity = 203/297 (68.35%), Postives = 233/297 (78.45%), Query Frame = 0


Query: 1   MARRFFRTSLSLSRSLMLHTEFHNPIPFPNLLKP-------IQSFPLTRPFFSSISQIDD 60
           M+RRFF    SLSRS +LH +F NPI  P  L P       I  FP+T  FFSS SQ+ D
Sbjct: 1   MSRRFFVGGASLSRSSILHAKFRNPISLPYFLHPNFPKSKSIHPFPITPRFFSSNSQMGD 60

Query: 61  A---LSPTILQPEKANRLELLRSLEFLFGSSFRSDPLVPNPSPLVIVISGPPGVGNDAVI 120
           A   LS  I  PE A+R ELLRSLEF   SSF S+PL+PNPSPL+IVISGP GVG DAVI
Sbjct: 61  ARRPLSANIPPPETADRSELLRSLEFSLNSSFSSEPLLPNPSPLIIVISGPSGVGKDAVI 120

Query: 121 KRLREVREGLHFAVTATTRPMRPGEVDGKDCYFVSKEEFLAMMKRNELLEYTSTYGNYKG 180
           KRLREVREGLHF VTAT+RPMRPGEVDG D YFVSKEEFLAM++RNELLEY   YG+YKG
Sbjct: 121 KRLREVREGLHFVVTATSRPMRPGEVDGVDYYFVSKEEFLAMIERNELLEYALVYGDYKG 180

Query: 181 TPKTQIRESMEKGYDIVLTVEIPRAKILRKILRDSAVFVFLKAESKVKLGERLIDRKAGT 240
            PK QIRE M +G+DIVL V+I  A+ L+KIL ++AVF+FL AES++KL ERLI+RK  T
Sbjct: 181 IPKKQIREFMARGHDIVLRVDIQGAETLKKILGNTAVFLFLMAESEIKLVERLINRKTET 240

Query: 241 EESLLVRLPLAREEVKHVKNFDYVVVNADGKLESAVKLVESIIDAEKAKVWQRKTVV 288
           +ESLLVR+  AREEVKHVK+FDYVVVN +G+LESAVKLVESIIDAEKAKVWQR  V+
Sbjct: 241 KESLLVRVATAREEVKHVKSFDYVVVNTEGELESAVKLVESIIDAEKAKVWQRNAVI 297

[Close]

BLAST of PI0016550.2 vs. NCBI nr
Match: XP_011658899.1 (guanylate kinase 3, chloroplastic [Cucumis sativus] >XP_011658900.1 guanylate kinase 3, chloroplastic [Cucumis sativus] >XP_031744600.1 guanylate kinase 3, chloroplastic [Cucumis sativus] >XP_031744601.1 guanylate kinase 3, chloroplastic [Cucumis sativus] >KGN43928.1 hypothetical protein Csa_017095 [Cucumis sativus])

HSP 1 Score: 449.1 bits (1154), Expect = 2.8e-122
Identity = 239/287 (83.28%), Postives = 253/287 (88.15%), Query Frame = 0


Query: 1   MARRFFRTSLSLSRSLMLHTEFHNPIPFPNLLKPIQSFPLTRPFFSSISQIDDALSPTIL 60
           MARRFF TSLSLSRSL  HT+F NPIPFPNL+KPIQSF LT PFFSS+SQI DALSPTI 
Sbjct: 1   MARRFFSTSLSLSRSLTRHTKFPNPIPFPNLIKPIQSFHLTPPFFSSVSQIGDALSPTIQ 60

Query: 61  QPEKANRLELLRSLEFLFGSSFRSDPLVPNPSPLVIVISGPPGVGNDAVIKRLREVREGL 120
            P++ NR ELLRSLE L GSSFRSD LV N SPLVIV+SGPPGVGNDAVIKRLREVREGL
Sbjct: 61  PPDEDNRSELLRSLEVLLGSSFRSDRLVYNQSPLVIVLSGPPGVGNDAVIKRLREVREGL 120

Query: 121 HFAVTATTRPMRPGEVDGKDCYFVSKEEFLAMMKRNELLEYTSTYGNYKGTPKTQIRESM 180
           HFAVTATTRPMRPGEVDGKDCYF+SKEEF AMMKRNELLE++  YG+YKG PKTQIRE M
Sbjct: 121 HFAVTATTRPMRPGEVDGKDCYFISKEEFSAMMKRNELLEFSFMYGHYKGIPKTQIREFM 180

Query: 181 EKGYDIVLTVEIPRAKILRKILRDSAVFVFLKAESKVKLGERLIDRKAGTEESLLVRLPL 240
           EKGYDIVL VEI RAKILRKILR+SAVFVFL AES+ KLGER IDRK GTEESLLVRLP 
Sbjct: 181 EKGYDIVLRVEISRAKILRKILRNSAVFVFLMAESEAKLGERSIDRKTGTEESLLVRLPW 240

Query: 241 AREEVKHVKNFDYVVVNADGKLESAVKLVESIIDAEKAKVWQRKTVV 288
            RE   +VKNFDYVVVN DGKLESAVKLVESIIDAEKAKV QR+TVV
Sbjct: 241 VRE---NVKNFDYVVVNTDGKLESAVKLVESIIDAEKAKVRQRRTVV 284

[Close]

BLAST of PI0016550.2 vs. NCBI nr
Match: XP_031739306.1 (guanylate kinase 3, chloroplastic [Cucumis sativus] >XP_031739307.1 guanylate kinase 3, chloroplastic [Cucumis sativus] >KAE8650366.1 hypothetical protein Csa_009762 [Cucumis sativus])

HSP 1 Score: 409.1 bits (1050), Expect = 3.3e-110
Identity = 225/297 (75.76%), Postives = 242/297 (81.48%), Query Frame = 0


Query: 1   MARRFFRTSLSLSRSLMLHTEFHNPIPFPNLL-------KPIQSFPLTRPFFSSISQIDD 60
           M+R FF T  +LSRS MLHT+FHNPIPFPN L       KPIQSFPLT PFFSSISQ+ D
Sbjct: 1   MSRIFFAT--TLSRSSMLHTKFHNPIPFPNFLNPISLKSKPIQSFPLTPPFFSSISQMGD 60

Query: 61  ALS---PTILQPEKANRLELLRSLEFLFGSSFRSDPLVPNPSPLVIVISGPPGVGNDAVI 120
           A     PTI  P+KA+R ELLRSLEF   SSF SDPLVPNPSPLVIVISGP GVG DAVI
Sbjct: 61  ARRPPFPTIPPPDKADRSELLRSLEFSLASSFSSDPLVPNPSPLVIVISGPSGVGKDAVI 120

Query: 121 KRLREVREGLHFAVTATTRPMRPGEVDGKDCYFVSKEEFLAMMKRNELLEYTSTYGNYKG 180
           KRLREVREGLHF VTAT+RPMRPGEVDGKD YFVSKE+FL M+ RNELLEY   YG+YKG
Sbjct: 121 KRLREVREGLHFVVTATSRPMRPGEVDGKDYYFVSKEDFLDMIARNELLEYALVYGDYKG 180

Query: 181 TPKTQIRESMEKGYDIVLTVEIPRAKILRKILRDSAVFVFLKAESKVKLGERLIDRKAGT 240
            PK QIRE M KGYDIVL V+   A+ LRK+L +SAVFVFL AES+VKL ERLIDRK  T
Sbjct: 181 IPKRQIREFMAKGYDIVLRVDTQGAETLRKVLGNSAVFVFLMAESEVKLVERLIDRKTET 240

Query: 241 EESLLVRLPLAREEVKHVKNFDYVVVNADGKLESAVKLVESIIDAEKAKVWQRKTVV 288
           +ESLLVR+  AREEVKHVKNFDYVVVNADGKLESAVKLVESIIDAEKAKV QR  VV
Sbjct: 241 KESLLVRVATAREEVKHVKNFDYVVVNADGKLESAVKLVESIIDAEKAKVLQRNAVV 295

[Close]

BLAST of PI0016550.2 vs. NCBI nr
Match: XP_008438733.1 (PREDICTED: guanylate kinase 3, chloroplastic-like [Cucumis melo] >XP_008438734.1 PREDICTED: guanylate kinase 3, chloroplastic-like [Cucumis melo] >XP_016899071.1 PREDICTED: guanylate kinase 3, chloroplastic-like [Cucumis melo])

HSP 1 Score: 406.8 bits (1044), Expect = 1.6e-109
Identity = 224/297 (75.42%), Postives = 241/297 (81.14%), Query Frame = 0


Query: 1   MARRFFRTSLSLSRSLMLHTEFHNPIPFPNLLKP-------IQSFPLTRPFFSSISQIDD 60
           M+RRFF T  SLSRS MLHT+FHNPIPFPN L P       IQSFPLT  FFSSISQ+ D
Sbjct: 1   MSRRFFAT--SLSRSSMLHTKFHNPIPFPNFLNPISLKSKTIQSFPLTPRFFSSISQMGD 60

Query: 61  ALS---PTILQPEKANRLELLRSLEFLFGSSFRSDPLVPNPSPLVIVISGPPGVGNDAVI 120
           A     PTI  P+KA+R ELLRSLEF  GSSF SDPLVPNPSPLVIVISGP GVG DAVI
Sbjct: 61  ARRPPFPTIPPPDKADRSELLRSLEFSLGSSFSSDPLVPNPSPLVIVISGPSGVGKDAVI 120

Query: 121 KRLREVREGLHFAVTATTRPMRPGEVDGKDCYFVSKEEFLAMMKRNELLEYTSTYGNYKG 180
           KRLREVREGLHF VTAT+RPMRPGEVDGKD YFVSKE+FL M+ RNELLEY   YG+YKG
Sbjct: 121 KRLREVREGLHFVVTATSRPMRPGEVDGKDYYFVSKEDFLDMIDRNELLEYALVYGDYKG 180

Query: 181 TPKTQIRESMEKGYDIVLTVEIPRAKILRKILRDSAVFVFLKAESKVKLGERLIDRKAGT 240
            PK QIRE M KGYDIVL V+   A  LRK+L +SAVFVFL AES+VKL ERLIDRK  T
Sbjct: 181 IPKRQIREFMAKGYDIVLRVDTQGAATLRKVLGNSAVFVFLMAESEVKLVERLIDRKTET 240

Query: 241 EESLLVRLPLAREEVKHVKNFDYVVVNADGKLESAVKLVESIIDAEKAKVWQRKTVV 288
           +ESLL+R+  AREEVKHV+NFDYVVVNADGKLESAVKLVESIIDAEKAKV QR  VV
Sbjct: 241 KESLLLRVATAREEVKHVRNFDYVVVNADGKLESAVKLVESIIDAEKAKVSQRNAVV 295

[Close]

BLAST of PI0016550.2 vs. NCBI nr
Match: XP_038880963.1 (guanylate kinase 3, chloroplastic-like isoform X1 [Benincasa hispida] >XP_038880964.1 guanylate kinase 3, chloroplastic-like isoform X2 [Benincasa hispida] >XP_038880965.1 guanylate kinase 3, chloroplastic-like isoform X2 [Benincasa hispida])

HSP 1 Score: 403.3 bits (1035), Expect = 1.8e-108
Identity = 223/297 (75.08%), Postives = 242/297 (81.48%), Query Frame = 0


Query: 1   MARRFFRTSLSLSRSLMLHTEFHNPIPFPNLL-------KPIQSFPLTRPFFSSISQIDD 60
           M+RRFF TSLSLS   +LHT+FHNPIPFPNLL       KPIQSFPLT PFFSSISQ+ D
Sbjct: 1   MSRRFFGTSLSLSS--VLHTKFHNPIPFPNLLNPIFPKSKPIQSFPLTPPFFSSISQMGD 60

Query: 61  A---LSPTILQPEKANRLELLRSLEFLFGSSFRSDPLVPNPSPLVIVISGPPGVGNDAVI 120
           A   L P I   ++  RLELLRSLEF   +SF SDP+VPNPSPLVIVISGP GVG DAVI
Sbjct: 61  ARGPLGPIIPPSDEVERLELLRSLEFSLNTSFSSDPMVPNPSPLVIVISGPSGVGKDAVI 120

Query: 121 KRLREVREGLHFAVTATTRPMRPGEVDGKDCYFVSKEEFLAMMKRNELLEYTSTYGNYKG 180
           KRLREVREGLHF VTAT+RPMRPGEVDGKD YFVSKEEFLAM++RNELLEY    G+ KG
Sbjct: 121 KRLREVREGLHFVVTATSRPMRPGEVDGKDNYFVSKEEFLAMIQRNELLEYALVCGHSKG 180

Query: 181 TPKTQIRESMEKGYDIVLTVEIPRAKILRKILRDSAVFVFLKAESKVKLGERLIDRKAGT 240
            PK QIRE M KGYDIVL+V I  A+ LRKILR+SAVFVFL AES+VKL ERLI+RK  T
Sbjct: 181 IPKRQIREFMGKGYDIVLSVNIQGAETLRKILRNSAVFVFLMAESEVKLVERLIERKTET 240

Query: 241 EESLLVRLPLAREEVKHVKNFDYVVVNADGKLESAVKLVESIIDAEKAKVWQRKTVV 288
           +ESLLV +  AREEVKHVKNFDYVVVNADGKLESAVKLVESIIDAEKAKV QR   V
Sbjct: 241 KESLLVTVAAAREEVKHVKNFDYVVVNADGKLESAVKLVESIIDAEKAKVCQRNAAV 295

[Close]

BLAST of PI0016550.2 vs. NCBI nr
Match: XP_038892980.1 (guanylate kinase 3, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 393.7 bits (1010), Expect = 1.4e-105
Identity = 215/281 (76.51%), Postives = 232/281 (82.56%), Query Frame = 0


Query: 17  MLHTEFHNPIPFPNL-------LKPIQSFPLTRPFFSSISQIDDA---LSPTILQPEKAN 76
           ML T+FHN IPF NL        KPIQSFPLT PFFSSISQ+ DA   LSP I   +KA+
Sbjct: 1   MLRTKFHNAIPFTNLRKPIFPKSKPIQSFPLTPPFFSSISQMGDARRPLSPAIPPLDKAD 60

Query: 77  RLELLRSLEFLFGSSFRSDPLVPNPSPLVIVISGPPGVGNDAVIKRLREVREGLHFAVTA 136
           RLELLRSLE    SSF SDPLVPNPSPLVIVISGP GVG DAVIKRLREVREGLHF VTA
Sbjct: 61  RLELLRSLESSLSSSFSSDPLVPNPSPLVIVISGPSGVGKDAVIKRLREVREGLHFVVTA 120

Query: 137 TTRPMRPGEVDGKDCYFVSKEEFLAMMKRNELLEYTSTYGNYKGTPKTQIRESMEKGYDI 196
           T+RPMR GEVDGKD YFVSKEEFLAM++RNELLEY S YG+YKG PK QIRE M KGYDI
Sbjct: 121 TSRPMRHGEVDGKDYYFVSKEEFLAMIERNELLEYASVYGDYKGIPKRQIREFMAKGYDI 180

Query: 197 VLTVEIPRAKILRKILRDSAVFVFLKAESKVKLGERLIDRKAGTEESLLVRLPLAREEVK 256
           VL V+I  A+ LRKIL +SAVFVFL AES+VKL ERLIDRK  T+ESLLVR+  AR+EV+
Sbjct: 181 VLRVDIQGAETLRKILGNSAVFVFLMAESEVKLVERLIDRKTETKESLLVRVATARKEVQ 240

Query: 257 HVKNFDYVVVNADGKLESAVKLVESIIDAEKAKVWQRKTVV 288
           HVKNFDYVVVNADGKL+SAVKLVESIIDAEKAKVWQR  VV
Sbjct: 241 HVKNFDYVVVNADGKLDSAVKLVESIIDAEKAKVWQRNAVV 281

[Close]

BLAST of PI0016550.2 vs. TAIR 10
Match: AT3G06200.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 288.5 bits (737), Expect = 6.0e-78
Identity = 160/276 (57.97%), Postives = 200/276 (72.46%), Query Frame = 0


Query: 18  LHTEFHNPIPFPNLLKPI---QSFP-----LTRPFFSSISQIDDAL--SPTILQPEKANR 77
           L + F   I FPN  KPI   +SFP      +   +SS+   D  +  +P     +   +
Sbjct: 5   LCSSFSRSIIFPN--KPIFIPRSFPQPLRHSSALSYSSLKMTDTHIPGNPNSSPIQSPTK 64

Query: 78  LELLRSLEFLFGSSFRSDPLVPNPSPLVIVISGPPGVGNDAVIKRLREVREGLHFAVTAT 137
            E LRSLEF  GSSF +DP++P P+ +VIVISGP GVG DAVI +LREVREGLHF VTAT
Sbjct: 65  PESLRSLEFQLGSSFTADPIIPPPNQIVIVISGPSGVGKDAVINKLREVREGLHFVVTAT 124

Query: 138 TRPMRPGEVDGKDCYFVSKEEFLAMMKRNELLEYTSTYGNYKGTPKTQIRESMEKGYDIV 197
           +RPMRPGEVDGKD +FVS+++FL+M++  ELLEY   YG YKG PK QI+E M KG DIV
Sbjct: 125 SRPMRPGEVDGKDYFFVSRDQFLSMVENEELLEYALVYGEYKGIPKKQIQEFMAKGEDIV 184

Query: 198 LTVEIPRAKILRKILRDSAVFVFLKAESKVKLGERLIDRKAGTEESLLVRLPLAREEVKH 257
           L V+I  A+ LR+IL +SAVF+FL AES++ + ERLIDRK  ++E LLVR+  AREEV+H
Sbjct: 185 LRVDIQGAQTLRRILGNSAVFIFLVAESELAMVERLIDRKTESQEELLVRVATAREEVRH 244

Query: 258 VKNFDYVVVNADGKLESAVKLVESIIDAEKAKVWQR 284
           +KNFDYVVVNA G+L+ AV  VESIIDAEK+KV QR
Sbjct: 245 LKNFDYVVVNAKGRLDDAVNRVESIIDAEKSKVHQR 278

[Close]

BLAST of PI0016550.2 vs. TAIR 10
Match: AT2G41880.1 (guanylate kinase 1 )

HSP 1 Score: 79.0 bits (193), Expect = 7.2e-15
Identity = 55/180 (30.56%), Postives = 92/180 (51.11%), Query Frame = 0


Query: 96  IVISGPPGVGNDAVIKRL-REVREGLHFAVTATTRPMRPGEVDGKDCYFVSKEEFLAMMK 155
           IVISGP GVG   +I  L +E      F+V+ TTR  R  E+DG   +F  K+     +K
Sbjct: 140 IVISGPSGVGKGTLISMLMKEFPSMFGFSVSHTTRSPRSMEMDGVHYHFADKKVMEKEIK 199

Query: 156 RNELLEYTSTYGNYKGTPKTQIRESMEKGYDIVLTVEIPRAKILRKILRDSAVFVFLKAE 215
             + LE+ S +GN  GT    +    + G   +L +++  A+ +R    D A+F+F+   
Sbjct: 200 DGKFLEFASVHGNLYGTSIESVEAVTDSGKRCILDIDVQGARSVRASSLD-AIFIFVCPP 259

Query: 216 SKVKLGERLIDRKAGTEESLLVRLPLAREEVKH-VKNFDYVVVNADGKLESAVKLVESII 274
           S  +L +RL  R   TEE +  RL  A  E+K  + +  + ++  +  LE   K +++++
Sbjct: 260 SMKELEDRLRARGTETEEQIQKRLRNAEAEIKEGISSGIFGLILYNDNLEECYKKLKNLL 318

[Close]

BLAST of PI0016550.2 vs. TAIR 10
Match: AT3G57550.1 (guanylate kinase )

HSP 1 Score: 73.2 bits (178), Expect = 4.0e-13
Identity = 56/182 (30.77%), Postives = 91/182 (50.00%), Query Frame = 0


Query: 96  IVISGPPGVGNDAVIKRL-REVREGLHFAVTATTRPMRPGEVDGKDCYFVSKEEFLAMMK 155
           IVISGP GVG   +I  L +E      F+V+ TTR  R  E +G   +F  K      +K
Sbjct: 140 IVISGPSGVGKGTLISMLMKEFPSMFGFSVSHTTRAPRCMEKNGVHYHFTDKTVMEKEIK 199

Query: 156 RNELLEYTSTYGNYKGTPKTQIRESMEKGYDIVLTVEIPRAKILRKILRDSAVFVFLKAE 215
             + LE+ S +GN  GT    +    + G   +L +++  A+ ++    D A+F+F+   
Sbjct: 200 DGKFLEFASVHGNLYGTSIESVEVVTDSGKRCILDIDVQGARSVKASSLD-AIFIFVCPP 259

Query: 216 SKVKLGERLIDRKAGTEESLLVRLPLAREEVKHVKN---FDYVVVNADGKLESAVKLVES 274
           S  +L +RL  R   TEE +  RL  A  E+K  K+   F++ + N    LE   K +++
Sbjct: 260 SMKELEDRLRARGTETEEQIQKRLRNADAEIKAGKSSGIFEHKLYN--DNLEECYKTLKN 318

[Close]

BLAST of PI0016550.2 vs. TAIR 10
Match: AT3G57550.2 (guanylate kinase )

HSP 1 Score: 73.2 bits (178), Expect = 4.0e-13
Identity = 56/182 (30.77%), Postives = 91/182 (50.00%), Query Frame = 0


Query: 96  IVISGPPGVGNDAVIKRL-REVREGLHFAVTATTRPMRPGEVDGKDCYFVSKEEFLAMMK 155
           IVISGP GVG   +I  L +E      F+V+ TTR  R  E +G   +F  K      +K
Sbjct: 140 IVISGPSGVGKGTLISMLMKEFPSMFGFSVSHTTRAPRCMEKNGVHYHFTDKTVMEKEIK 199

Query: 156 RNELLEYTSTYGNYKGTPKTQIRESMEKGYDIVLTVEIPRAKILRKILRDSAVFVFLKAE 215
             + LE+ S +GN  GT    +    + G   +L +++  A+ ++    D A+F+F+   
Sbjct: 200 DGKFLEFASVHGNLYGTSIESVEVVTDSGKRCILDIDVQGARSVKASSLD-AIFIFVCPP 259

Query: 216 SKVKLGERLIDRKAGTEESLLVRLPLAREEVKHVKN---FDYVVVNADGKLESAVKLVES 274
           S  +L +RL  R   TEE +  RL  A  E+K  K+   F++ + N    LE   K +++
Sbjct: 260 SMKELEDRLRARGTETEEQIQKRLRNADAEIKAGKSSGIFEHKLYN--DNLEECYKTLKN 318

The following BLAST results are available for this feature:

BLAST of PI0016550.2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94JM2	8.4e-77	57.97	Guanylate kinase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=GK3 PE=2 SV...	[more]
Q10M74	4.3e-73	55.48	Guanylate kinase 2, chloroplastic/mitochondrial OS=Oryza sativa subsp. japonica ...	[more]
Q3ZWD8	1.6e-35	40.84	Guanylate kinase OS=Dehalococcoides mccartyi (strain CBDB1) OX=255470 GN=gmk PE=...	[more]
Q3ZAF9	3.0e-34	40.96	Guanylate kinase OS=Dehalococcoides mccartyi (strain ATCC BAA-2266 / KCTC 15142 ...	[more]
Q895Q5	4.0e-34	41.12	Guanylate kinase OS=Clostridium tetani (strain Massachusetts / E88) OX=212717 GN...	[more]

BLAST of PI0016550.2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K367	1.4e-122	83.28	Guanylate kinase-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Cs...	[more]
A0A1S3AWS3	7.8e-110	75.42	Guanylate kinase OS=Cucumis melo OX=3656 GN=LOC103483755 PE=3 SV=1	[more]
A0A6J1INA9	3.8e-104	72.26	Guanylate kinase OS=Cucurbita maxima OX=3661 GN=LOC111479039 PE=3 SV=1	[more]
A0A6J1GV20	6.4e-104	72.26	Guanylate kinase OS=Cucurbita moschata OX=3662 GN=LOC111457439 PE=3 SV=1	[more]
A0A6J1C9N3	4.3e-100	68.35	Guanylate kinase OS=Momordica charantia OX=3673 GN=LOC111009480 PE=3 SV=1	[more]

BLAST of PI0016550.2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011658899.1	2.8e-122	83.28	guanylate kinase 3, chloroplastic [Cucumis sativus] >XP_011658900.1 guanylate ki...	[more]
XP_031739306.1	3.3e-110	75.76	guanylate kinase 3, chloroplastic [Cucumis sativus] >XP_031739307.1 guanylate ki...	[more]
XP_008438733.1	1.6e-109	75.42	PREDICTED: guanylate kinase 3, chloroplastic-like [Cucumis melo] >XP_008438734.1...	[more]
XP_038880963.1	1.8e-108	75.08	guanylate kinase 3, chloroplastic-like isoform X1 [Benincasa hispida] >XP_038880...	[more]
XP_038892980.1	1.4e-105	76.51	guanylate kinase 3, chloroplastic-like [Benincasa hispida]	[more]

BLAST of PI0016550.2 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G06200.1	6.0e-78	57.97	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT2G41880.1	7.2e-15	30.56	guanylate kinase 1	[more]
AT3G57550.1	4.0e-13	30.77	guanylate kinase	[more]
AT3G57550.2	4.0e-13	30.77	guanylate kinase	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008145	Guanylate kinase/L-type calcium channel beta subunit	SMART	SM00072	gk_7	coord: 92..277 e-value: 1.6E-37 score: 140.7
IPR008145	Guanylate kinase/L-type calcium channel beta subunit	PFAM	PF00625	Guanylate_kin	coord: 95..275 e-value: 9.3E-38 score: 129.7
IPR017665	Guanylate kinase	TIGRFAM	TIGR03263	TIGR03263	coord: 95..274 e-value: 1.6E-48 score: 162.6
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 88..287 e-value: 2.0E-47 score: 163.3
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 91..280
None	No IPR available	PANTHER	PTHR23117	GUANYLATE KINASE-RELATED	coord: 53..284
None	No IPR available	PANTHER	PTHR23117:SF13	GUANYLATE KINASE	coord: 53..284
None	No IPR available	CDD	cd00071	GMPK	coord: 95..212 e-value: 4.45315E-42 score: 139.59
IPR008144	Guanylate kinase-like domain	PROSITE	PS50052	GUANYLATE_KINASE_2	coord: 93..274 score: 28.983843

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
PI0016550	PI0016550	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0016550.2-five_prime_utr	PI0016550.2-five_prime_utr-chr01:494442..494507	five_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0016550.2-exon	PI0016550.2-exon-chr01:494442..495399	exon
PI0016550.2-exon	PI0016550.2-exon-chr01:496544..497887	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0016550.2-cds	PI0016550.2-cds-chr01:494508..495383	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0016550.2-three_prime_utr	PI0016550.2-three_prime_utr-chr01:495384..495399	three_prime_UTR
PI0016550.2-three_prime_utr	PI0016550.2-three_prime_utr-chr01:496544..497887	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
PI0016550.2	PI0016550.2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006163	purine nucleotide metabolic process
biological_process	GO:0046710	GDP metabolic process
biological_process	GO:0046037	GMP metabolic process
biological_process	GO:0016310	phosphorylation
cellular_component	GO:0005829	cytosol
molecular_function	GO:0004385	guanylate kinase activity
molecular_function	GO:0005524	ATP binding