PI0013838.1 (mRNA) Melon (PI 482460) v1

BLAST of PI0013838.1 vs. ExPASy Swiss-Prot
Match: P30181 (DNA topoisomerase 1 alpha OS=Arabidopsis thaliana OX=3702 GN=TOP1A PE=1 SV=1)

HSP 1 Score: 1013.4 bits (2619), Expect = 1.5e-294
Identity = 574/943 (60.87%), Postives = 687/943 (72.85%), Query Frame = 0

Query: 1   MAVETFGKAPMFDD---DDEDNMPIVFKRSS--ASRQNQLDSEIKHSQAQRLNKLPGRQG 60
           M  ET  K P+ D+   D +D+ P+ FKR++  AS  NQ  S  + S+A    K+   + 
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMR- 60

Query: 61  SDLCSTNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSSPIQEPLTKSLGTSLKASDSMD 120
           S + S NG + +N        KTS ++S + S  +SK+SP + PL   + +++K    + 
Sbjct: 61  SPVTSPNGTTPSN--------KTSIVKSSMPS-SSSKASPAKSPLRNDMPSTVKDRSQLQ 120

Query: 121 SRPKNLVNAAVKEENVSIKPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTVSKNKVKKS 180
                      K+++   +   E+ DSEDD+PLS  L GN    +   VS  S    KK+
Sbjct: 121 -----------KDQS---ECKIEHEDSEDDRPLSSILSGNKGPTSSRQVS--SPQPEKKN 180

Query: 181 SGD-------------SDDEVPLSSKFQMKYASGVLGSKQHGFDEKKPLVPKVHQNGSTS 240
           +GD             SDDE P+SS F+ K  SG+ G  Q   DEKKPLV K+HQNGST 
Sbjct: 181 NGDRPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTV 240

Query: 241 RDKLQKPAVLSSKRPLPNADSHSPSQFSTKKPKLSDSSSPLSSK--------------LV 300
           ++++    VL  KRPL    + S  Q S KK K+S S + +  K              LV
Sbjct: 241 KNEVPNGKVL-GKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLV 300

Query: 301 S--LKAKQ-------EVDDDDDHIPISQRMKMVSATVN-------------KSSSSKPAV 360
           S  +KAKQ         DDDDD +PIS+R K  S+  N              SS++KP  
Sbjct: 301 SPKMKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKA 360

Query: 361 SKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQTHGV 420
             VVS   +   K +KK  K SKY  S+K  PS+GDGQKKWTTLVHNGVIFPPPY+ HG+
Sbjct: 361 RNVVSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGI 420

Query: 421 KMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVIQNLDDC 480
           K+LYKGKPVDLT +QEEVATMFAVM++TDY  KP+F+ENFWNDWR+LLGK HVIQ LDDC
Sbjct: 421 KILYKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDC 480

Query: 481 DFTPIYDWYQMEKEKKKQMTTEEKKTLKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPGL 540
           DFTPIY+W+  EKEKKKQM+TEEKK LKEEK+KQEEKYMWA++DGVKEK+GNFRVEPPGL
Sbjct: 481 DFTPIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGL 540

Query: 541 FRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDP 600
           FRGRGEHPKMGKLK+RI P +IT+NIGKGAPIPECPI GERWKEV+HDNTVTWLAFW DP
Sbjct: 541 FRGRGEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADP 600

Query: 601 INQKEFKYVFLAASSSLKGQSDKEKYEKARMLKVN------AYTKDFTSKDPTRRQIAVA 660
           IN KEFKYVFL A SSLKG SDKEKYEKAR L  +       YTK+FT+KD   RQIAVA
Sbjct: 601 INPKEFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVA 660

Query: 661 TYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYENTVEVE 720
           TYLIDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE
Sbjct: 661 TYLIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVE 720

Query: 721 LPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNR 780
             V+KAI QFQ+GKS TDDLFD+LDT+KLNAHLK+L+PGLTAKVFRTYNASITLD+ML++
Sbjct: 721 PLVYKAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQ 780

Query: 781 GTKDGDVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDL 840
            TKDGDV++KIV YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL+++LKELK +L
Sbjct: 781 ETKDGDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNL 840

Query: 841 DRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKI 884
           DRAKKGKPPL+GSDGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKI
Sbjct: 841 DRAKKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKI 900

BLAST of PI0013838.1 vs. ExPASy Swiss-Prot
Match: Q9FJ79 (DNA topoisomerase 1 beta OS=Arabidopsis thaliana OX=3702 GN=TOP1B PE=1 SV=1)

HSP 1 Score: 1011.1 bits (2613), Expect = 7.5e-294
Identity = 579/942 (61.46%), Postives = 685/942 (72.72%), Query Frame = 0

Query: 1   MAVETFGKAPM------FDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQ 60
           MA E F K  +      ++D+DED++P+VFKR+S             + A   N+ P   
Sbjct: 1   MATEAFVKPVVPNGHDGYEDEDEDDIPLVFKRNS-------------NTAATTNR-PSPI 60

Query: 61  GSDLCSTNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSSPIQEPLTKSLGTSLKASDSM 120
            + + ++   S+ +   M S P TSP RS   S ++S   P   P + S+  S   S   
Sbjct: 61  NNAMRNSAIGSTKSSPPMRS-PLTSPNRSASSSTRSSMMKPAL-PSSSSVQRSTLKSPLR 120

Query: 121 DSRPKNLVNAAVKEENVSIK-PS---QENSDSEDDKPLSIRLKGNSNQPNK-----GLVS 180
           D R     +   KE N   K PS    ++ DSEDDKPLS RLK +S +  K     G  S
Sbjct: 121 DDR-----SVVAKERNGFGKAPSVSKSDDEDSEDDKPLSARLKLDSKEVTKQPSSSGRGS 180

Query: 181 T---VSKNKVKKSSGDS-------DDEVPLSSKFQMKYASGVLGSKQHGFDEKKPLVPKV 240
           T   V K+ ++             D+  P+SS  Q K + G   SK    ++K+PLV  +
Sbjct: 181 TQQAVQKSNMRPQGLSDYTKKKVLDERAPMSSTVQTKTSVGTSSSKPVHIEQKRPLVNNI 240

Query: 241 HQNG---STSRDKLQKPAVLSSKRPLPNADSHSPSQFSTKKPKLS--------------- 300
            +NG    T     Q PA    KRPL      S +Q S K+PKLS               
Sbjct: 241 DRNGLKPKTEGHSSQAPA----KRPLEKGS--SSNQSSVKRPKLSEPARPVKVEQGSHIS 300

Query: 301 ----------DSSSPLSSKLVSLKAKQEVDDDDDHIPISQRMKMVSATVNKSSSSKPAVS 360
                     D+S PL +   ++  K++  D DDH+PI+ RMK  S+  NKSSS+KP+ S
Sbjct: 301 ATQDAKGKNLDASKPLRANQATV--KEDNSDGDDHVPIASRMKSDSSN-NKSSSAKPSSS 360

Query: 361 KVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQTHGVK 420
           K+++SS +   +K  K VK SKY KS+K LPS GDGQKKW TL HNGVIFPPPY+ HGVK
Sbjct: 361 KMIASSSRTIAQKPNKWVKDSKYSKSSKSLPS-GDGQKKWKTLQHNGVIFPPPYKRHGVK 420

Query: 421 MLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVIQNLDDCD 480
           +L++GKPVDLTP+QEEVATMFAVM++T+Y  KPKF+ENFWNDWRKLLGKNH+I++LDDCD
Sbjct: 421 ILFQGKPVDLTPEQEEVATMFAVMRETEYYKKPKFRENFWNDWRKLLGKNHMIKSLDDCD 480

Query: 481 FTPIYDWYQMEKEKKKQMTTEEKKTLKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPGLF 540
           FTPIY+WY  EKE KKQMT EEK+ +KEEKLKQEEKYMWA+LDGV+E++GNFRVEPPGLF
Sbjct: 481 FTPIYEWYMQEKETKKQMTAEEKRIVKEEKLKQEEKYMWAVLDGVRERIGNFRVEPPGLF 540

Query: 541 RGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDPI 600
           RGRGEHPKMGKLK+RIRP DITINIGK APIPECPIPGERWKEV+HDNTVTWLAFW+DPI
Sbjct: 541 RGRGEHPKMGKLKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSDPI 600

Query: 601 NQKEFKYVFLAASSSLKGQSDKEKYEKARMLKVN------AYTKDFTSKDPTRRQIAVAT 660
           N KEFKYVFLAASSSLKGQSDKEKYEKAR L  +      AYTKDF +KD T+RQIAVAT
Sbjct: 601 NPKEFKYVFLAASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAVAT 660

Query: 661 YLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYENTVEVEL 720
           YLIDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN L+F+FLGKDSI+Y NTVEVE 
Sbjct: 661 YLIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKLKFDFLGKDSIQYVNTVEVEP 720

Query: 721 PVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRG 780
            V+KAI QFQ+GKS TDDLFD+LDT+KLN HLK+LM GLTAKVFRTYNASITLD ML++ 
Sbjct: 721 LVYKAIGQFQAGKSKTDDLFDELDTSKLNTHLKELMAGLTAKVFRTYNASITLDLMLSKE 780

Query: 781 TKDGDVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLD 840
           T+DGDV EK+V YQ ANKEVAIICNHQRTVSKSHGAQ+ +L  KI ELR+ +KEL IDLD
Sbjct: 781 TRDGDVPEKVVVYQQANKEVAIICNHQRTVSKSHGAQVEKLAVKIEELREQIKELNIDLD 840

Query: 841 RAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKIN 884
           RAKKG+ PL GSDGK+KRNLTPEALEKKI QT  KIEK+ERDM+TKED+KTVALGTSKIN
Sbjct: 841 RAKKGRTPLMGSDGKRKRNLTPEALEKKIMQTQGKIEKMERDMQTKEDMKTVALGTSKIN 900

BLAST of PI0013838.1 vs. ExPASy Swiss-Prot
Match: P93119 (DNA topoisomerase 1 OS=Daucus carota OX=4039 GN=TOP1 PE=2 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 5.4e-236
Identity = 478/810 (59.01%), Postives = 571/810 (70.49%), Query Frame = 0

Query: 120 NLVNAAVKEENVSIKPSQENSDSEDDKPLSIR-----LKGNSNQ----PNKGLVSTVSKN 179
           N++   +KEE  S   S+   DS+DDKPLS +     L GNS+      N    S  +  
Sbjct: 16  NVMEKTLKEEGQSRSNSE---DSDDDKPLSHKLSSGALNGNSHHIRMGSNLSCPSPYTSP 75

Query: 180 KVKKSSGDSDDEVPLSSKFQMKYASGVLGSKQHGFDEKKPLVPKVHQNGSTSRDKLQKPA 239
           K +   G  +DE+ LSS+ Q+   +G   ++    DE KPL             +L+ P+
Sbjct: 76  KPRIIKG-PEDEMLLSSELQLN--AGASNAESSDSDESKPLA-----------SELEDPS 135

Query: 240 VLSSKRPLPNADSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDHIPISQRMK 299
              +KRPL    +  PS   +KK KLS+ S+P + KL + + ++   DDD    IS R K
Sbjct: 136 SSLNKRPLIQPKNSDPS--PSKKAKLSEPSAPTNRKLKTAEQEEAAADDDP--SISNRNK 195

Query: 300 MVSATVNKSSSSKPAVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTL 359
             +   +K S  K      VS+S+ K        V  SK +K   +L    +GQ     L
Sbjct: 196 KSTTPASKVSDKKKRPD--VSASVNK--------VDFSKSLKVPLVLVKGRNGQ----PL 255

Query: 360 VHNGVIFPPPYQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDW 419
           VHNGVIFPP Y+ HGVKMLY+  PVDLTP+QEEVATMFAVM +T+YM KPKF+ENF +DW
Sbjct: 256 VHNGVIFPPLYKPHGVKMLYRENPVDLTPEQEEVATMFAVMLETEYMTKPKFRENFMSDW 315

Query: 420 RKLLGKNHVIQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKTLKEEKLK-QEEKYMWAIL 479
           RK+LG+ H+IQNL+DCDFTPIY+W+Q EKEKKKQM+T+EKK +KE K + ++EKYMWA++
Sbjct: 316 RKILGEKHIIQNLEDCDFTPIYEWHQREKEKKKQMSTDEKKAIKEGKDETRKEKYMWAVV 375

Query: 480 DGV-KEKVGNFRVEPPGLFRGRGEHPKMGKLKRRIRPS-DITINIGKGAPIPECPIPGER 539
           DG+ +EKVGNFRV    + +      K  ++K+ +    DITINIGK APIPE PIPGER
Sbjct: 376 DGMSREKVGNFRVGTTRVVQRSWRASKDPQVKKNVYSQIDITINIGKDAPIPEPPIPGER 435

Query: 540 WKEVRHDNTVTWLAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLK-------V 599
           WKE+RHDNTVTWLAFWNDPIN KEFKYVFLAASSSLKG+  +EKYEK+R LK        
Sbjct: 436 WKEIRHDNTVTWLAFWNDPINPKEFKYVFLAASSSLKGKVTREKYEKSRKLKDYIEGIRA 495

Query: 600 NAYTKDFTSKDPTRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNL 659
            AYTKDF SKD  +RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENV+   PN+
Sbjct: 496 AAYTKDFASKDSKKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVETKRPNI 555

Query: 660 LEFNFLGKDSIRYENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGL 719
           L+F+FLGKDSIRY+N VEVE  VF AI QF+SGK G DDLFD+LDT+KLNAHLK+LMPGL
Sbjct: 556 LKFDFLGKDSIRYQNEVEVEPRVFSAIEQFRSGKEGGDDLFDQLDTSKLNAHLKELMPGL 615

Query: 720 TAKVFRTYNASITLDDM-----LNRGTKDGDVSEKIVTYQHANKEVAIICNHQRTVSKSH 779
           TAKVFRTYNASITLD+M     L+R TK GDV+EK+V YQHANKEVAIICNHQRTVSKSH
Sbjct: 616 TAKVFRTYNASITLDEMVRCKLLSRETKGGDVAEKVVVYQHANKEVAIICNHQRTVSKSH 675

Query: 780 GAQMTRLNEKISELRDILKELKIDLDRAKKGKPPLKGSDGKQKRNLTPE----------- 839
            AQM RLNEKI EL+ +LKEL+ DL R  KGKPPLK SDGK KRNL  +           
Sbjct: 676 SAQMVRLNEKIEELKGLLKELQEDLTRVNKGKPPLKNSDGKPKRNLNLKRYKGKLLKLIQ 735

Query: 840 -----------ALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAWCK 884
                      ++ KKI+QTN KIEK+ERD +TKEDLKTVALGTSKINYLDPRITVAWCK
Sbjct: 736 KLRKWSETRRLSVTKKIAQTNTKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCK 790

BLAST of PI0013838.1 vs. ExPASy Swiss-Prot
Match: Q54RC3 (DNA topoisomerase 1 OS=Dictyostelium discoideum OX=44689 GN=top1 PE=3 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 5.8e-145
Identity = 344/880 (39.09%), Postives = 483/880 (54.89%), Query Frame = 0

Query: 70  KRMTSLPKTSPMRSE-IGSQKASKSSPIQEPLTKSLGTSLKASDSMDSRPKNLVNAAVKE 129
           K++T  PK +P+  +   +    K  P ++P+  S       SDS DS   +  +++  +
Sbjct: 59  KKLTPTPKPAPVTPKPTAAVNGKKPIPKKKPVDSS-----SDSDSSDSSSDSDSDSSDSD 118

Query: 130 ENVSIKPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTVSKNKVKKSSGDSDDEVPLSSK 189
            + S   S ++S S+ D P    +    N+P+ G VS  SKN  K ++  +     + SK
Sbjct: 119 SDSS---SSDSSSSDSDVPKKKPISKIPNKPS-GTVSKTSKNSPKTTTTTTTTTTTVKSK 178

Query: 190 FQMKYASGVLGSKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPNADSHSPSQ 249
                                   PKV    S+S D     +  SS       DS S S 
Sbjct: 179 ----------------------PAPKVKPEQSSSDDSSSDSSDDSSD------DSSSDSS 238

Query: 250 FSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDHIPISQRMKMVSATVNKSSSSKPAVSK 309
              +KPK   S  P + K   +  K+E        P+ + +++   T   ++        
Sbjct: 239 SEEEKPKKKPSKKPAAVK-KEITVKKETTTTTKKTPVKKEVEVKKETKTTTTKKVKKEES 298

Query: 310 VVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQK-------------KWTTLVHNGV 369
                 +KT  K K   +K K  +  +      +G               KWTTL+HNG 
Sbjct: 299 DDDGEDEKTTTKKKTTTRKRKLKEEEEGEEEEEEGVNKWWDKEDDEDDGTKWTTLIHNGP 358

Query: 370 IFPPPYQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLL- 429
           +FPP Y  HG+K +Y GK V LTP+QEEVAT +A   +TD++ K  FK+NF+ +++ LL 
Sbjct: 359 VFPPAYVPHGIKFMYDGKVVKLTPKQEEVATFYANYLETDHVKKQAFKDNFFKEFKGLLT 418

Query: 430 -GKNHVIQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKTLKEEKLKQEEKYMWAILDGVK 489
             +  +++ +D CDF+ I+   + +KE++K  + EEK     EK K +EK+ +A +DG K
Sbjct: 419 SEQKEIVKKMDKCDFSHIHTHLKTKKEQRKARSKEEKDAETAEKKKIQEKHGFATIDGFK 478

Query: 490 EKVGNFRVEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRH 549
           EKVGN+ +EPPGLF GRG+HPK G LK+RI PSD+T+NIG+G  IP  PIPGE+WKE+ H
Sbjct: 479 EKVGNYMIEPPGLFLGRGQHPKTGMLKQRIMPSDVTLNIGEGEVIPPSPIPGEKWKEIVH 538

Query: 550 DNTVTWLAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLK------VNAYTKDF 609
           +NTVTWLAFW + +N   FKYV+LA SS +KGQ+D++K+E AR LK         Y K F
Sbjct: 539 NNTVTWLAFWRESVN-GGFKYVWLAPSSKIKGQADRKKFEVARGLKNCIGDIRKGYQKSF 598

Query: 610 TSKDPTRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLG 669
             +   +RQ+AVA YLID LALR GNEK +D+ADTVGCC+L+VE++K    N +  +FLG
Sbjct: 599 NDESTEKRQLAVALYLIDFLALRVGNEKGEDQADTVGCCSLRVEHIKLESNNTITLDFLG 658

Query: 670 KDSIRYENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRT 729
           KDS+RY NTV++    +K +  F  GK  TD LFD L   +LN HLK  M GL+AKVFRT
Sbjct: 659 KDSMRYLNTVQIREDAYKVLKTFVKGKKPTDLLFDSLTVTQLNNHLKKQMTGLSAKVFRT 718

Query: 730 YNASITLDDMLNR-GTKD-GDVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEK 789
           YNASITL   L++  TK+   + EKI+ Y   N+EVAI+CNHQ+   K+    M++++EK
Sbjct: 719 YNASITLQKELDKMDTKEFQSIDEKILFYNRCNREVAILCNHQKAPPKNLAETMSKIDEK 778

Query: 790 ISELRDILKELKIDLDRAKKGKPPLKGSDG-----------------KQKRNLTPEALEK 849
           I ELRD+      DL R K GK P    +                    K++   E  +K
Sbjct: 779 IQELRDLQ-----DLCRIKIGKTPKSQQEDIESREAERLPKLIQLAITNKKDKDKEKTDK 838

Query: 850 KIS------------------QTNAKIEKIERDMKT-------KEDLKTVALGTSKINYL 884
           +IS                  +   KI K E  +K        K++LKTVALGTSKINYL
Sbjct: 839 EISFDVKAAFEKSRTLPDAKGKIKEKIAKYEEQIKKQEILKTGKDELKTVALGTSKINYL 893

BLAST of PI0013838.1 vs. ExPASy Swiss-Prot
Match: P07799 (DNA topoisomerase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=top1 PE=1 SV=2)

HSP 1 Score: 513.5 bits (1321), Expect = 4.9e-144
Identity = 337/840 (40.12%), Postives = 465/840 (55.36%), Query Frame = 0

Query: 129 ENVSIKPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTVSKNKVKKSSGDSDDEVPLSSK 188
           + +S+K S E SDS ++ PLS                  S NK  KS  D DD +P+  +
Sbjct: 23  KRISMKESDEESDSSENHPLS-----------------ESLNKKSKSESDEDD-IPIRKR 82

Query: 189 FQMKYASGVLGSKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPNADSHSPSQ 248
                      SK++  +       KV  NG     K  K AV+  +         SP  
Sbjct: 83  --------RASSKKNMSNSSSKKRAKVMGNGGLKNGK--KTAVVKEEEDFNEIAKPSPKH 142

Query: 249 FSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDHIPISQRMKMVSATVNKSSSSKPAVSK 308
               K   S + +  +        K+E  D DD +P+ + +  VS T  KS     A   
Sbjct: 143 KRVSKANGSKNGAKSA-------VKKEESDTDDSVPL-RAVSTVSLTPYKSELPSGA--- 202

Query: 309 VVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQ--THGV 368
             S++  ++    +   +  K+  S  +     D  +KWTTL HNGVIF PPY+     V
Sbjct: 203 --STTQNRSPNDEEDEDEDYKWWTSENI-----DDTQKWTTLEHNGVIFAPPYEPLPKNV 262

Query: 369 KMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGK---NHVIQNL 428
           K++Y G PV+L P+ EEVA  +A M +TD+   P F++NF+ D+ K+  +   NH I+  
Sbjct: 263 KLIYDGNPVNLPPEAEEVAGFYAAMLETDHAKNPVFQDNFFRDFLKVCDECNFNHNIKEF 322

Query: 429 DDCDFTPIYDWYQMEKEKKKQMTTEEKKTLKEEKLKQEEKYMWAILDGVKEKVGNFRVEP 488
             CDFT ++  ++ ++E+KK M  E+KK +K++K ++EEKY W ILDG KEKVGNFR+EP
Sbjct: 323 SKCDFTQMFHHFEQKREEKKSMPKEQKKAIKQKKDEEEEKYKWCILDGRKEKVGNFRIEP 382

Query: 489 PGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFW 548
           PGLFRGRG HPK G LKRR+ P  ITINIG+G P+PE P+PG +W EV+HDNTVTWLA W
Sbjct: 383 PGLFRGRGSHPKTGSLKRRVYPEQITINIGEGVPVPE-PLPGHQWAEVKHDNTVTWLATW 442

Query: 549 NDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLK------VNAYTKDFTSKDPTRRQI 608
           ++ IN    KYVFLAA SSLKGQSD +KYEK+R LK         Y KD  S+    RQ 
Sbjct: 443 HENIN-NNVKYVFLAAGSSLKGQSDLKKYEKSRKLKDYIDDIRKGYRKDLKSELTVERQR 502

Query: 609 AVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYENTV 668
             A YLID  ALRAGNEK +DEADTVGCC+L+ E+V   PP  + F+FLGKDSIRY N V
Sbjct: 503 GTAMYLIDVFALRAGNEKGEDEADTVGCCSLRYEHVTLKPPRTVVFDFLGKDSIRYYNEV 562

Query: 669 EVELPVFKAITQF-QSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDD 728
           EV+  VFK +  F +  K   D +FD+L TN LN +L  LM GL+AKVFRTYNAS T+ +
Sbjct: 563 EVDPQVFKNLKIFKRPPKKEGDLIFDRLSTNSLNKYLTSLMDGLSAKVFRTYNASYTMAE 622

Query: 729 MLNRGTKDGDVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISEL---RDIL 788
            L +  K+  +++KI+ Y  AN+ VAI+CNHQR+V+K+H  QM R  E+I  L   R  L
Sbjct: 623 ELKKMPKNLTLADKILFYNRANRTVAILCNHQRSVTKNHDVQMERFAERIKALQYQRMRL 682

Query: 789 KELKIDLD-RAKKGKPP------------------------------------------- 848
           +++ ++L+ +  K KP                                            
Sbjct: 683 RKMMLNLEPKLAKSKPELLAKEEGITDSWIVKHHETLYELEKEKIKKKFDRENEKLAAED 742

Query: 849 -------------LKGSDGKQK--------RNLTP-----EALEKKISQTNAKIEKIERD 884
                        LK +D  +K        + + P     E LEK++++ N +I  +   
Sbjct: 743 PKSVLPESELEVRLKAADELKKALDAELKSKKVDPGRSSMEQLEKRLNKLNERINVMRTQ 802

BLAST of PI0013838.1 vs. ExPASy TrEMBL
Match: A0A1S3BM72 (DNA topoisomerase I OS=Cucumis melo OX=3656 GN=LOC103491068 PE=3 SV=1)

HSP 1 Score: 1672.5 bits (4330), Expect = 0.0e+00
Identity = 863/889 (97.08%), Postives = 869/889 (97.75%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLCS 60
           MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLCS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLCS 60

Query: 61  TNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSSPIQEPLTKSLGTSLKASDSMDSRPKN 120
           TNGQSSNNQKRMTSLPKTSPMRSEIGSQKA KSSPIQEPLTKSLG SLKASDSMDSRPKN
Sbjct: 61  TNGQSSNNQKRMTSLPKTSPMRSEIGSQKAPKSSPIQEPLTKSLGISLKASDSMDSRPKN 120

Query: 121 LVNAAVKEENVSIKPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTVSKNKVKKSSGDSD 180
           LVNAAVKEENVSI PSQENSDSEDDKPLSIRLKGNSNQPNKGLVST+ KNKVKKSSGDSD
Sbjct: 121 LVNAAVKEENVSINPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTIPKNKVKKSSGDSD 180

Query: 181 DEVPLSSKFQMKYASGVLGSKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPN 240
           DEVPLSSKFQMK+ASG +GSKQ GFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPN
Sbjct: 181 DEVPLSSKFQMKHASGPMGSKQLGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPN 240

Query: 241 ADSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDHIPISQRMKMVSATVNKSS 300
            DSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDD IPISQR+K   ATVNKSS
Sbjct: 241 EDSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDDIPISQRLKKTPATVNKSS 300

Query: 301 SSKPAVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
           SSKP VSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP
Sbjct: 301 SSKPTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360

Query: 361 YQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420
           YQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420

Query: 421 QNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKTLKEEKLKQEEKYMWAILDGVKEKVGNFR 480
           QNLDDCDFTPIYDWYQMEKEKKKQMTTEEKK LKEEKLKQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480

Query: 481 VEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540
           VEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540

Query: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLK------VNAYTKDFTSKDPTR 600
           AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKAR+LK       NAYTKDFTSKDPTR
Sbjct: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARVLKDYIKNIRNAYTKDFTSKDPTR 600

Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYE 660
           RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYE 660

Query: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITL 720
           NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITL 720

Query: 721 DDMLNRGTKDGDVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780
           DDMLNRGTKDG+VSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK
Sbjct: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780

Query: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
           ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840

Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 889

BLAST of PI0013838.1 vs. ExPASy TrEMBL
Match: A0A5A7VHI9 (DNA topoisomerase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G002170 PE=3 SV=1)

HSP 1 Score: 1661.4 bits (4301), Expect = 0.0e+00
Identity = 863/907 (95.15%), Postives = 869/907 (95.81%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLCS 60
           MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLCS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLCS 60

Query: 61  TNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSSPIQEPLTKSLGTSLKASDSMDSRPKN 120
           TNGQSSNNQKRMTSLPKTSPMRSEIGSQKA KSSPIQEPLTKSLG SLKASDSMDSRPKN
Sbjct: 61  TNGQSSNNQKRMTSLPKTSPMRSEIGSQKAPKSSPIQEPLTKSLGISLKASDSMDSRPKN 120

Query: 121 LVNAAVKEENVSIKPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTVSKNKVKKSSGDSD 180
           LVNAAVKEENVSI PSQENSDSEDDKPLSIRLKGNSNQPNKGLVST+ KNKVKKSSGDSD
Sbjct: 121 LVNAAVKEENVSINPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTIPKNKVKKSSGDSD 180

Query: 181 DEVPLSSKFQMKYASGVLGSKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPN 240
           DEVPLSSKFQMK+ASG +GSKQ GFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPN
Sbjct: 181 DEVPLSSKFQMKHASGPMGSKQLGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPN 240

Query: 241 ADSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDHIPISQRMKMVSATVNKSS 300
            DSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDD IPISQR+K   ATVNKSS
Sbjct: 241 EDSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDDIPISQRLKKTPATVNKSS 300

Query: 301 SSKPAVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
           SSKP VSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP
Sbjct: 301 SSKPTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360

Query: 361 YQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420
           YQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420

Query: 421 QNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKTLKEEKLKQEEKYMWAILDGVKEKVGNFR 480
           QNLDDCDFTPIYDWYQMEKEKKKQMTTEEKK LKEEKLKQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480

Query: 481 VEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540
           VEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540

Query: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLK------VNAYTKDFTSKDPTR 600
           AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKAR+LK       NAYTKDFTSKDPTR
Sbjct: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARVLKDYIKNIRNAYTKDFTSKDPTR 600

Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYE 660
           RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYE 660

Query: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITL 720
           NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITL 720

Query: 721 DDMLNRGTKDGDVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780
           DDMLNRGTKDG+VSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK
Sbjct: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780

Query: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
           ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840

Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEK------------------IFNKSLLAKFAWAMD 884
           LGTSKINYLDPRITVAWCKRHEVPIEK                  IFNKSLLAKFAWAMD
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKFKNFLLIFVIHNHNLLPQIFNKSLLAKFAWAMD 900

BLAST of PI0013838.1 vs. ExPASy TrEMBL
Match: A0A0A0L2W2 (DNA topoisomerase I OS=Cucumis sativus OX=3659 GN=Csa_3G063680 PE=3 SV=1)

HSP 1 Score: 1656.3 bits (4288), Expect = 0.0e+00
Identity = 854/889 (96.06%), Postives = 866/889 (97.41%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLCS 60
           MAVETFGKAPMFDDDDEDNMPIVFKR+SASRQNQLDSEIKHSQAQRL+KLPGRQGSDLCS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRTSASRQNQLDSEIKHSQAQRLSKLPGRQGSDLCS 60

Query: 61  TNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSSPIQEPLTKSLGTSLKASDSMDSRPKN 120
           TNGQSSNNQKRMTSLPKTSPMRSEIGSQKAS+SSPI EPLTKSLGTSLKAS SMDSRPK+
Sbjct: 61  TNGQSSNNQKRMTSLPKTSPMRSEIGSQKASRSSPIPEPLTKSLGTSLKASHSMDSRPKS 120

Query: 121 LVNAAVKEENVSIKPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTVSKNKVKKSSGDSD 180
           LVNA VKEENVSI PSQENSDSEDDKPLSIRLKGN NQPNKGLVSTV KNKVKKS  DSD
Sbjct: 121 LVNAVVKEENVSINPSQENSDSEDDKPLSIRLKGNPNQPNKGLVSTVPKNKVKKSLADSD 180

Query: 181 DEVPLSSKFQMKYASGVLGSKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPN 240
           DEVPLSSKFQMK+ASGVLGSKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPN
Sbjct: 181 DEVPLSSKFQMKHASGVLGSKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPN 240

Query: 241 ADSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDHIPISQRMKMVSATVNKSS 300
            DSHSPSQF TKKPKL+DSSSPL+SKLVSLKAKQEVDDDDD IPISQRMK VSATVNKSS
Sbjct: 241 EDSHSPSQFPTKKPKLTDSSSPLNSKLVSLKAKQEVDDDDDDIPISQRMKKVSATVNKSS 300

Query: 301 SSKPAVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
           SSKP +SKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP
Sbjct: 301 SSKPTISKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360

Query: 361 YQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420
           YQTHGVKMLYKGKPVDLTP QEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKMLYKGKPVDLTPPQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420

Query: 421 QNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKTLKEEKLKQEEKYMWAILDGVKEKVGNFR 480
           QNLDDCDFTPIYDWYQMEKEKKKQMTTEEKK +KEEKLKQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKAVKEEKLKQEEKYMWAILDGVKEKVGNFR 480

Query: 481 VEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540
           VEPPGLFRGRGEHPKMGKLKRRI PSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKRRICPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540

Query: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLK------VNAYTKDFTSKDPTR 600
           AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLK       NAYTKDFTSKDPTR
Sbjct: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPTR 600

Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYE 660
           RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYE 660

Query: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITL 720
           NTVEVELPVFKAITQFQSGKSGTDDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720

Query: 721 DDMLNRGTKDGDVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780
           DDMLNRGTK+G+VSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK
Sbjct: 721 DDMLNRGTKNGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780

Query: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
           ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840

Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 889

BLAST of PI0013838.1 vs. ExPASy TrEMBL
Match: A0A6J1KZE0 (DNA topoisomerase I OS=Cucurbita maxima OX=3661 GN=LOC111500229 PE=3 SV=1)

HSP 1 Score: 1577.0 bits (4082), Expect = 0.0e+00
Identity = 818/889 (92.01%), Postives = 843/889 (94.83%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLCS 60
           MAVETFGKA MFDDDDEDNMPIVFKRSSASRQNQL+SE+KHSQ+QRLN+L GR GSD CS
Sbjct: 1   MAVETFGKATMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60

Query: 61  TNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSSPIQEPLTKSLGTSLKASDSMDSRPKN 120
           TNGQSSN QKRMTSLPKTSPMR EIGSQKASKSS IQEPL KSLGT+LK SDSMDSRPK 
Sbjct: 61  TNGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSS-IQEPLVKSLGTTLKVSDSMDSRPKQ 120

Query: 121 LVNAAVKEENVSIKPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTVSKNKVKKSSGDSD 180
           LVNAAVKEEN SI P+QENSDSEDDKPLSIRLKGNSN P+KG + TV KNK+KKSS DSD
Sbjct: 121 LVNAAVKEENDSIHPAQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSD 180

Query: 181 DEVPLSSKFQMKYASGVLGSKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPN 240
           DEVPLSSKF+MK ASGVLG KQHGFDEKKPL  KVHQNGSTS DKLQKPAV+SSKRPLPN
Sbjct: 181 DEVPLSSKFRMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSSDKLQKPAVVSSKRPLPN 240

Query: 241 ADSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDHIPISQRMKMVSATVNKSS 300
           ADSHS SQ S+KKPKLSDSS+PLS+  VSLKAKQEV DDDDHIPISQRMKM SA VNKSS
Sbjct: 241 ADSHSSSQISSKKPKLSDSSTPLSTHTVSLKAKQEVVDDDDHIPISQRMKM-SAAVNKSS 300

Query: 301 SSKPAVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
            SK  VSKV SSSIKKT KKSKKPVKKSKYVKSTKLLPS+GDGQKKWTTLVHNGVIFPPP
Sbjct: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360

Query: 361 YQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420
           YQTHGVK+L+KGKPVDLTP+QEEVATMFAVMKDTDYM KPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420

Query: 421 QNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKTLKEEKLKQEEKYMWAILDGVKEKVGNFR 480
           QNLDDCDFTPIYDWYQ EKEKKKQMTTEEKK LKEEKLKQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480

Query: 481 VEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540
           VEPPGLFRGRGEHPKMGKLK+RIRPSDITINIGK APIPECPIPG+RWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWL 540

Query: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLK------VNAYTKDFTSKDPTR 600
           AFWNDPIN KEFKYVFLAASSSLKGQSDKEKYEKARMLK       N+YTKDFTSKDPTR
Sbjct: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTR 600

Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYE 660
           RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSI+YE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660

Query: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITL 720
           NTVEVELPVFKAITQFQSGKSG+DDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720

Query: 721 DDMLNRGTKDGDVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780
           DDMLNRGTKDG+VSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK
Sbjct: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780

Query: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
           ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840

Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887

BLAST of PI0013838.1 vs. ExPASy TrEMBL
Match: A0A6J1EWI0 (DNA topoisomerase I OS=Cucurbita moschata OX=3662 GN=LOC111438616 PE=3 SV=1)

HSP 1 Score: 1573.9 bits (4074), Expect = 0.0e+00
Identity = 816/889 (91.79%), Postives = 841/889 (94.60%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLCS 60
           MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQL+SE+KHSQ+QRLN+L GR GSD CS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60

Query: 61  TNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSSPIQEPLTKSLGTSLKASDSMDSRPKN 120
            NGQSSN QKRMTSLPKTSPMR EIGSQKA+KSS IQEPL KSLGT+LK SDSMDSRPK 
Sbjct: 61  ANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSS-IQEPLVKSLGTTLKVSDSMDSRPKQ 120

Query: 121 LVNAAVKEENVSIKPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTVSKNKVKKSSGDSD 180
           L NAAVKEEN SI P+QENSDSEDDKPLSIRLKGNSN P+KG + TV KNK+KKSS DSD
Sbjct: 121 LANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSD 180

Query: 181 DEVPLSSKFQMKYASGVLGSKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPN 240
           DEVPLSSKFQMK ASGVLG KQHGF EKKPL  KVHQNGSTSRDKLQKPAV+SSKRPLPN
Sbjct: 181 DEVPLSSKFQMKSASGVLGGKQHGFHEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPN 240

Query: 241 ADSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDHIPISQRMKMVSATVNKSS 300
           ADSHS SQ S+KKPKLSDSS+PLS+  VSLKAKQEV DDDDHIPISQRMKM SA VNKSS
Sbjct: 241 ADSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRMKM-SAAVNKSS 300

Query: 301 SSKPAVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
            SK  VSKV SSSIKKT KKSKKPVKKSKYVKSTKLLPS+GDGQKKWTTLVHNGVIFPPP
Sbjct: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360

Query: 361 YQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420
           YQTHGVK+L+KGKPVDLT +QEEVATMFAVMKDTDYM KPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKILHKGKPVDLTTEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420

Query: 421 QNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKTLKEEKLKQEEKYMWAILDGVKEKVGNFR 480
           QNLDDCDFTPIYDWYQ EKEKKKQMTTEEKK LKEEKLKQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480

Query: 481 VEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540
           VEPPGLFRGRGEHPKMGKLK+RIRPSDITINIGK APIPECPIPG+RWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWL 540

Query: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLK------VNAYTKDFTSKDPTR 600
           AFWNDPIN KEFKYVFLAASSSLKGQSDKEKYEKARMLK       N+YTKDFTSKDPTR
Sbjct: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTR 600

Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYE 660
           RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSI+YE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660

Query: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITL 720
           NTVEVELPVFKAITQFQSGKSG+DDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720

Query: 721 DDMLNRGTKDGDVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780
           DDMLNRGTKDG+VSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK
Sbjct: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780

Query: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
           ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840

Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887

BLAST of PI0013838.1 vs. NCBI nr
Match: XP_008449103.1 (PREDICTED: DNA topoisomerase 1-like [Cucumis melo] >XP_008449104.1 PREDICTED: DNA topoisomerase 1-like [Cucumis melo])

HSP 1 Score: 1672.5 bits (4330), Expect = 0.0e+00
Identity = 863/889 (97.08%), Postives = 869/889 (97.75%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLCS 60
           MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLCS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLCS 60

Query: 61  TNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSSPIQEPLTKSLGTSLKASDSMDSRPKN 120
           TNGQSSNNQKRMTSLPKTSPMRSEIGSQKA KSSPIQEPLTKSLG SLKASDSMDSRPKN
Sbjct: 61  TNGQSSNNQKRMTSLPKTSPMRSEIGSQKAPKSSPIQEPLTKSLGISLKASDSMDSRPKN 120

Query: 121 LVNAAVKEENVSIKPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTVSKNKVKKSSGDSD 180
           LVNAAVKEENVSI PSQENSDSEDDKPLSIRLKGNSNQPNKGLVST+ KNKVKKSSGDSD
Sbjct: 121 LVNAAVKEENVSINPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTIPKNKVKKSSGDSD 180

Query: 181 DEVPLSSKFQMKYASGVLGSKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPN 240
           DEVPLSSKFQMK+ASG +GSKQ GFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPN
Sbjct: 181 DEVPLSSKFQMKHASGPMGSKQLGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPN 240

Query: 241 ADSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDHIPISQRMKMVSATVNKSS 300
            DSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDD IPISQR+K   ATVNKSS
Sbjct: 241 EDSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDDIPISQRLKKTPATVNKSS 300

Query: 301 SSKPAVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
           SSKP VSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP
Sbjct: 301 SSKPTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360

Query: 361 YQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420
           YQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420

Query: 421 QNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKTLKEEKLKQEEKYMWAILDGVKEKVGNFR 480
           QNLDDCDFTPIYDWYQMEKEKKKQMTTEEKK LKEEKLKQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480

Query: 481 VEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540
           VEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540

Query: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLK------VNAYTKDFTSKDPTR 600
           AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKAR+LK       NAYTKDFTSKDPTR
Sbjct: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARVLKDYIKNIRNAYTKDFTSKDPTR 600

Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYE 660
           RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYE 660

Query: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITL 720
           NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITL 720

Query: 721 DDMLNRGTKDGDVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780
           DDMLNRGTKDG+VSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK
Sbjct: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780

Query: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
           ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840

Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 889

BLAST of PI0013838.1 vs. NCBI nr
Match: KAA0065816.1 (DNA topoisomerase 1-like [Cucumis melo var. makuwa] >TYJ96555.1 DNA topoisomerase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1661.4 bits (4301), Expect = 0.0e+00
Identity = 863/907 (95.15%), Postives = 869/907 (95.81%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLCS 60
           MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLCS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLCS 60

Query: 61  TNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSSPIQEPLTKSLGTSLKASDSMDSRPKN 120
           TNGQSSNNQKRMTSLPKTSPMRSEIGSQKA KSSPIQEPLTKSLG SLKASDSMDSRPKN
Sbjct: 61  TNGQSSNNQKRMTSLPKTSPMRSEIGSQKAPKSSPIQEPLTKSLGISLKASDSMDSRPKN 120

Query: 121 LVNAAVKEENVSIKPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTVSKNKVKKSSGDSD 180
           LVNAAVKEENVSI PSQENSDSEDDKPLSIRLKGNSNQPNKGLVST+ KNKVKKSSGDSD
Sbjct: 121 LVNAAVKEENVSINPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTIPKNKVKKSSGDSD 180

Query: 181 DEVPLSSKFQMKYASGVLGSKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPN 240
           DEVPLSSKFQMK+ASG +GSKQ GFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPN
Sbjct: 181 DEVPLSSKFQMKHASGPMGSKQLGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPN 240

Query: 241 ADSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDHIPISQRMKMVSATVNKSS 300
            DSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDD IPISQR+K   ATVNKSS
Sbjct: 241 EDSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDDIPISQRLKKTPATVNKSS 300

Query: 301 SSKPAVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
           SSKP VSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP
Sbjct: 301 SSKPTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360

Query: 361 YQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420
           YQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420

Query: 421 QNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKTLKEEKLKQEEKYMWAILDGVKEKVGNFR 480
           QNLDDCDFTPIYDWYQMEKEKKKQMTTEEKK LKEEKLKQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480

Query: 481 VEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540
           VEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540

Query: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLK------VNAYTKDFTSKDPTR 600
           AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKAR+LK       NAYTKDFTSKDPTR
Sbjct: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARVLKDYIKNIRNAYTKDFTSKDPTR 600

Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYE 660
           RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYE 660

Query: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITL 720
           NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITL 720

Query: 721 DDMLNRGTKDGDVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780
           DDMLNRGTKDG+VSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK
Sbjct: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780

Query: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
           ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840

Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEK------------------IFNKSLLAKFAWAMD 884
           LGTSKINYLDPRITVAWCKRHEVPIEK                  IFNKSLLAKFAWAMD
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKFKNFLLIFVIHNHNLLPQIFNKSLLAKFAWAMD 900

BLAST of PI0013838.1 vs. NCBI nr
Match: XP_004149585.1 (DNA topoisomerase 1 alpha [Cucumis sativus] >XP_011650506.1 DNA topoisomerase 1 alpha [Cucumis sativus] >KGN56068.1 hypothetical protein Csa_010762 [Cucumis sativus])

HSP 1 Score: 1656.3 bits (4288), Expect = 0.0e+00
Identity = 854/889 (96.06%), Postives = 866/889 (97.41%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLCS 60
           MAVETFGKAPMFDDDDEDNMPIVFKR+SASRQNQLDSEIKHSQAQRL+KLPGRQGSDLCS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRTSASRQNQLDSEIKHSQAQRLSKLPGRQGSDLCS 60

Query: 61  TNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSSPIQEPLTKSLGTSLKASDSMDSRPKN 120
           TNGQSSNNQKRMTSLPKTSPMRSEIGSQKAS+SSPI EPLTKSLGTSLKAS SMDSRPK+
Sbjct: 61  TNGQSSNNQKRMTSLPKTSPMRSEIGSQKASRSSPIPEPLTKSLGTSLKASHSMDSRPKS 120

Query: 121 LVNAAVKEENVSIKPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTVSKNKVKKSSGDSD 180
           LVNA VKEENVSI PSQENSDSEDDKPLSIRLKGN NQPNKGLVSTV KNKVKKS  DSD
Sbjct: 121 LVNAVVKEENVSINPSQENSDSEDDKPLSIRLKGNPNQPNKGLVSTVPKNKVKKSLADSD 180

Query: 181 DEVPLSSKFQMKYASGVLGSKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPN 240
           DEVPLSSKFQMK+ASGVLGSKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPN
Sbjct: 181 DEVPLSSKFQMKHASGVLGSKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPN 240

Query: 241 ADSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDHIPISQRMKMVSATVNKSS 300
            DSHSPSQF TKKPKL+DSSSPL+SKLVSLKAKQEVDDDDD IPISQRMK VSATVNKSS
Sbjct: 241 EDSHSPSQFPTKKPKLTDSSSPLNSKLVSLKAKQEVDDDDDDIPISQRMKKVSATVNKSS 300

Query: 301 SSKPAVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
           SSKP +SKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP
Sbjct: 301 SSKPTISKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360

Query: 361 YQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420
           YQTHGVKMLYKGKPVDLTP QEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKMLYKGKPVDLTPPQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420

Query: 421 QNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKTLKEEKLKQEEKYMWAILDGVKEKVGNFR 480
           QNLDDCDFTPIYDWYQMEKEKKKQMTTEEKK +KEEKLKQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKAVKEEKLKQEEKYMWAILDGVKEKVGNFR 480

Query: 481 VEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540
           VEPPGLFRGRGEHPKMGKLKRRI PSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKRRICPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540

Query: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLK------VNAYTKDFTSKDPTR 600
           AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLK       NAYTKDFTSKDPTR
Sbjct: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPTR 600

Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYE 660
           RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYE 660

Query: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITL 720
           NTVEVELPVFKAITQFQSGKSGTDDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720

Query: 721 DDMLNRGTKDGDVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780
           DDMLNRGTK+G+VSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK
Sbjct: 721 DDMLNRGTKNGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780

Query: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
           ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840

Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 889

BLAST of PI0013838.1 vs. NCBI nr
Match: XP_038904656.1 (DNA topoisomerase 1 alpha-like isoform X1 [Benincasa hispida] >XP_038904657.1 DNA topoisomerase 1 alpha-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1605.9 bits (4157), Expect = 0.0e+00
Identity = 833/890 (93.60%), Postives = 853/890 (95.84%), Query Frame = 0

Query: 1   MAVETFGKAPMF-DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLC 60
           MAVETFGKAPMF DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQ+QRLNKL GRQGSD+C
Sbjct: 1   MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQGSDIC 60

Query: 61  STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSSPIQEPLTKSLGTSLKASDSMDSRPK 120
           STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASK SPI+EP  K LGTSLK SDSMDSR K
Sbjct: 61  STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASK-SPIREPPVKPLGTSLKVSDSMDSRSK 120

Query: 121 NLVNAAVKEENVSIKPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTVSKNKVKKSSGDS 180
            LVN AVK+ENVSI  +QENSDSEDDKPLSIRLKGNS+QPNKGLV TV KNKVKKSSGDS
Sbjct: 121 PLVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGNSSQPNKGLVPTVPKNKVKKSSGDS 180

Query: 181 DDEVPLSSKFQMKYASGVLGSKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240
           DDE+PLSSKFQMK  SGVLGSKQHGFDEKKPLVP+VHQNGSTSRDKLQKPAVLSSKRPLP
Sbjct: 181 DDEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLP 240

Query: 241 NADSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDHIPISQRMKMVSATVNKS 300
           NADSH+PSQ STKKPKLSDSS+PLSSKLVSLKAKQEVDD+DD IPISQRMK +SA VNKS
Sbjct: 241 NADSHTPSQISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKS 300

Query: 301 SSSKPAVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
           SSSK  VSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP
Sbjct: 301 SSSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360

Query: 361 PYQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420
           PYQTHGVK+LYKGKPVDLTP+QEEVATMFAVMKDT+YMLKPKFKENFWNDWRKLLGKNHV
Sbjct: 361 PYQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHV 420

Query: 421 IQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKTLKEEKLKQEEKYMWAILDGVKEKVGNF 480
           IQNLDDCDFTPIYDWYQ EKEKKKQMTTEEKK LKEEKLKQEEKYMWAILDGVKEKVGNF
Sbjct: 421 IQNLDDCDFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF 480

Query: 481 RVEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW 540
           RVEPPGLFRGRGEHPKMGKLKRRI PSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW
Sbjct: 481 RVEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW 540

Query: 541 LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLK------VNAYTKDFTSKDPT 600
           LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLK       NAYTKDFTSKD T
Sbjct: 541 LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDST 600

Query: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRY 660
           RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVK VPPNLLEFNFLGKDSI+Y
Sbjct: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKY 660

Query: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASIT 720
           ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASIT
Sbjct: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT 720

Query: 721 LDDMLNRGTKDGDVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIL 780
           LDDMLNRGTKDGDV EKI TYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+
Sbjct: 721 LDDMLNRGTKDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIV 780

Query: 781 KELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840
           KELKIDLDRAKKGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV
Sbjct: 781 KELKIDLDRAKKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840

Query: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 889

BLAST of PI0013838.1 vs. NCBI nr
Match: XP_023553297.1 (DNA topoisomerase 1 beta-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1577.8 bits (4084), Expect = 0.0e+00
Identity = 816/889 (91.79%), Postives = 843/889 (94.83%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLCS 60
           MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQL+SE+KHSQ+QRLN+L GR GSD CS
Sbjct: 1   MAVETFGKAPMFDDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDACS 60

Query: 61  TNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSSPIQEPLTKSLGTSLKASDSMDSRPKN 120
            NGQSSN QKRMTSLPKTSPMR EIGSQKASKSS IQEPL KSLGT+LK SDSMDSRPK 
Sbjct: 61  ANGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSS-IQEPLVKSLGTTLKVSDSMDSRPKQ 120

Query: 121 LVNAAVKEENVSIKPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTVSKNKVKKSSGDSD 180
           L NAAVKEEN SI P+Q+NSDSEDDKPLSIRLKGNSN P+KG + TV KNK+KKSS DSD
Sbjct: 121 LANAAVKEENDSIHPTQDNSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSD 180

Query: 181 DEVPLSSKFQMKYASGVLGSKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLPN 240
           DEVPLSSKFQMK ASGVLG +QHGFDEKKPL  KVHQNGSTSRDKLQKPAV+SSKRPLPN
Sbjct: 181 DEVPLSSKFQMKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPN 240

Query: 241 ADSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDHIPISQRMKMVSATVNKSS 300
           ADSHS SQ S+KKPKLSDSS+PLS+  VSLKAKQEV DDDDHIPISQRMKM SA VNKSS
Sbjct: 241 ADSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVADDDDHIPISQRMKM-SAAVNKSS 300

Query: 301 SSKPAVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
            SK  VSKV SSSIKKT KKSKKPVKKSKYVKSTKLLPS+GDGQKKWTTLVHNGVIFPPP
Sbjct: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360

Query: 361 YQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVI 420
           YQTHGVK+L+KGKPVDLTP+QEEVATMFAVMKDTDYM KPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420

Query: 421 QNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKTLKEEKLKQEEKYMWAILDGVKEKVGNFR 480
           QNLDDCDFTPIYDWYQ EKEKKKQMTTEEKK LKEEKLKQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480

Query: 481 VEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540
           VEPPGLFRGRGEHPKMGKLK+RIRPSDITINIGK APIPECPIPG+RWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWL 540

Query: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLK------VNAYTKDFTSKDPTR 600
           AFWNDPIN KEFKYVFLAASSSLKGQSDKEKYEKARMLK       N+YTKDFTSKDPTR
Sbjct: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTR 600

Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYE 660
           RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSI+YE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660

Query: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITL 720
           NTVEVELPVFKAITQFQSGKSG+DDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720

Query: 721 DDMLNRGTKDGDVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780
           DDMLNRGTKDG+VSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+K
Sbjct: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVK 780

Query: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
           ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840

Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887

BLAST of PI0013838.1 vs. TAIR 10
Match: AT5G55300.1 (DNA topoisomerase I alpha )

HSP 1 Score: 1013.4 bits (2619), Expect = 1.1e-295
Identity = 574/943 (60.87%), Postives = 687/943 (72.85%), Query Frame = 0

Query: 1   MAVETFGKAPMFDD---DDEDNMPIVFKRSS--ASRQNQLDSEIKHSQAQRLNKLPGRQG 60
           M  ET  K P+ D+   D +D+ P+ FKR++  AS  NQ  S  + S+A    K+   + 
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMR- 60

Query: 61  SDLCSTNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSSPIQEPLTKSLGTSLKASDSMD 120
           S + S NG + +N        KTS ++S + S  +SK+SP + PL   + +++K    + 
Sbjct: 61  SPVTSPNGTTPSN--------KTSIVKSSMPS-SSSKASPAKSPLRNDMPSTVKDRSQLQ 120

Query: 121 SRPKNLVNAAVKEENVSIKPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTVSKNKVKKS 180
                      K+++   +   E+ DSEDD+PLS  L GN    +   VS  S    KK+
Sbjct: 121 -----------KDQS---ECKIEHEDSEDDRPLSSILSGNKGPTSSRQVS--SPQPEKKN 180

Query: 181 SGD-------------SDDEVPLSSKFQMKYASGVLGSKQHGFDEKKPLVPKVHQNGSTS 240
           +GD             SDDE P+SS F+ K  SG+ G  Q   DEKKPLV K+HQNGST 
Sbjct: 181 NGDRPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTV 240

Query: 241 RDKLQKPAVLSSKRPLPNADSHSPSQFSTKKPKLSDSSSPLSSK--------------LV 300
           ++++    VL  KRPL    + S  Q S KK K+S S + +  K              LV
Sbjct: 241 KNEVPNGKVL-GKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLV 300

Query: 301 S--LKAKQ-------EVDDDDDHIPISQRMKMVSATVN-------------KSSSSKPAV 360
           S  +KAKQ         DDDDD +PIS+R K  S+  N              SS++KP  
Sbjct: 301 SPKMKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKA 360

Query: 361 SKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQTHGV 420
             VVS   +   K +KK  K SKY  S+K  PS+GDGQKKWTTLVHNGVIFPPPY+ HG+
Sbjct: 361 RNVVSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGI 420

Query: 421 KMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVIQNLDDC 480
           K+LYKGKPVDLT +QEEVATMFAVM++TDY  KP+F+ENFWNDWR+LLGK HVIQ LDDC
Sbjct: 421 KILYKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDC 480

Query: 481 DFTPIYDWYQMEKEKKKQMTTEEKKTLKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPGL 540
           DFTPIY+W+  EKEKKKQM+TEEKK LKEEK+KQEEKYMWA++DGVKEK+GNFRVEPPGL
Sbjct: 481 DFTPIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGL 540

Query: 541 FRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDP 600
           FRGRGEHPKMGKLK+RI P +IT+NIGKGAPIPECPI GERWKEV+HDNTVTWLAFW DP
Sbjct: 541 FRGRGEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADP 600

Query: 601 INQKEFKYVFLAASSSLKGQSDKEKYEKARMLKVN------AYTKDFTSKDPTRRQIAVA 660
           IN KEFKYVFL A SSLKG SDKEKYEKAR L  +       YTK+FT+KD   RQIAVA
Sbjct: 601 INPKEFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVA 660

Query: 661 TYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYENTVEVE 720
           TYLIDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE
Sbjct: 661 TYLIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVE 720

Query: 721 LPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNR 780
             V+KAI QFQ+GKS TDDLFD+LDT+KLNAHLK+L+PGLTAKVFRTYNASITLD+ML++
Sbjct: 721 PLVYKAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQ 780

Query: 781 GTKDGDVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDL 840
            TKDGDV++KIV YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL+++LKELK +L
Sbjct: 781 ETKDGDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNL 840

Query: 841 DRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKI 884
           DRAKKGKPPL+GSDGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKI
Sbjct: 841 DRAKKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKI 900

BLAST of PI0013838.1 vs. TAIR 10
Match: AT5G55310.1 (DNA topoisomerase 1 beta )

HSP 1 Score: 1011.1 bits (2613), Expect = 5.3e-295
Identity = 579/942 (61.46%), Postives = 685/942 (72.72%), Query Frame = 0

Query: 1   MAVETFGKAPM------FDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQ 60
           MA E F K  +      ++D+DED++P+VFKR+S             + A   N+ P   
Sbjct: 1   MATEAFVKPVVPNGHDGYEDEDEDDIPLVFKRNS-------------NTAATTNR-PSPI 60

Query: 61  GSDLCSTNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSSPIQEPLTKSLGTSLKASDSM 120
            + + ++   S+ +   M S P TSP RS   S ++S   P   P + S+  S   S   
Sbjct: 61  NNAMRNSAIGSTKSSPPMRS-PLTSPNRSASSSTRSSMMKPAL-PSSSSVQRSTLKSPLR 120

Query: 121 DSRPKNLVNAAVKEENVSIK-PS---QENSDSEDDKPLSIRLKGNSNQPNK-----GLVS 180
           D R     +   KE N   K PS    ++ DSEDDKPLS RLK +S +  K     G  S
Sbjct: 121 DDR-----SVVAKERNGFGKAPSVSKSDDEDSEDDKPLSARLKLDSKEVTKQPSSSGRGS 180

Query: 181 T---VSKNKVKKSSGDS-------DDEVPLSSKFQMKYASGVLGSKQHGFDEKKPLVPKV 240
           T   V K+ ++             D+  P+SS  Q K + G   SK    ++K+PLV  +
Sbjct: 181 TQQAVQKSNMRPQGLSDYTKKKVLDERAPMSSTVQTKTSVGTSSSKPVHIEQKRPLVNNI 240

Query: 241 HQNG---STSRDKLQKPAVLSSKRPLPNADSHSPSQFSTKKPKLS--------------- 300
            +NG    T     Q PA    KRPL      S +Q S K+PKLS               
Sbjct: 241 DRNGLKPKTEGHSSQAPA----KRPLEKGS--SSNQSSVKRPKLSEPARPVKVEQGSHIS 300

Query: 301 ----------DSSSPLSSKLVSLKAKQEVDDDDDHIPISQRMKMVSATVNKSSSSKPAVS 360
                     D+S PL +   ++  K++  D DDH+PI+ RMK  S+  NKSSS+KP+ S
Sbjct: 301 ATQDAKGKNLDASKPLRANQATV--KEDNSDGDDHVPIASRMKSDSSN-NKSSSAKPSSS 360

Query: 361 KVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQTHGVK 420
           K+++SS +   +K  K VK SKY KS+K LPS GDGQKKW TL HNGVIFPPPY+ HGVK
Sbjct: 361 KMIASSSRTIAQKPNKWVKDSKYSKSSKSLPS-GDGQKKWKTLQHNGVIFPPPYKRHGVK 420

Query: 421 MLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVIQNLDDCD 480
           +L++GKPVDLTP+QEEVATMFAVM++T+Y  KPKF+ENFWNDWRKLLGKNH+I++LDDCD
Sbjct: 421 ILFQGKPVDLTPEQEEVATMFAVMRETEYYKKPKFRENFWNDWRKLLGKNHMIKSLDDCD 480

Query: 481 FTPIYDWYQMEKEKKKQMTTEEKKTLKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPGLF 540
           FTPIY+WY  EKE KKQMT EEK+ +KEEKLKQEEKYMWA+LDGV+E++GNFRVEPPGLF
Sbjct: 481 FTPIYEWYMQEKETKKQMTAEEKRIVKEEKLKQEEKYMWAVLDGVRERIGNFRVEPPGLF 540

Query: 541 RGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDPI 600
           RGRGEHPKMGKLK+RIRP DITINIGK APIPECPIPGERWKEV+HDNTVTWLAFW+DPI
Sbjct: 541 RGRGEHPKMGKLKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSDPI 600

Query: 601 NQKEFKYVFLAASSSLKGQSDKEKYEKARMLKVN------AYTKDFTSKDPTRRQIAVAT 660
           N KEFKYVFLAASSSLKGQSDKEKYEKAR L  +      AYTKDF +KD T+RQIAVAT
Sbjct: 601 NPKEFKYVFLAASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAVAT 660

Query: 661 YLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYENTVEVEL 720
           YLIDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN L+F+FLGKDSI+Y NTVEVE 
Sbjct: 661 YLIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKLKFDFLGKDSIQYVNTVEVEP 720

Query: 721 PVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRG 780
            V+KAI QFQ+GKS TDDLFD+LDT+KLN HLK+LM GLTAKVFRTYNASITLD ML++ 
Sbjct: 721 LVYKAIGQFQAGKSKTDDLFDELDTSKLNTHLKELMAGLTAKVFRTYNASITLDLMLSKE 780

Query: 781 TKDGDVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLD 840
           T+DGDV EK+V YQ ANKEVAIICNHQRTVSKSHGAQ+ +L  KI ELR+ +KEL IDLD
Sbjct: 781 TRDGDVPEKVVVYQQANKEVAIICNHQRTVSKSHGAQVEKLAVKIEELREQIKELNIDLD 840

Query: 841 RAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKIN 884
           RAKKG+ PL GSDGK+KRNLTPEALEKKI QT  KIEK+ERDM+TKED+KTVALGTSKIN
Sbjct: 841 RAKKGRTPLMGSDGKRKRNLTPEALEKKIMQTQGKIEKMERDMQTKEDMKTVALGTSKIN 900

BLAST of PI0013838.1 vs. TAIR 10
Match: AT5G55300.2 (DNA topoisomerase I alpha )

HSP 1 Score: 1008.4 bits (2606), Expect = 3.4e-294
Identity = 572/940 (60.85%), Postives = 684/940 (72.77%), Query Frame = 0

Query: 1   MAVETFGKAPMFDD---DDEDNMPIVFKRSS--ASRQNQLDSEIKHSQAQRLNKLPGRQG 60
           M  ET  K P+ D+   D +D+ P+ FKR++  AS  NQ  S  + S+A    K+   + 
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMR- 60

Query: 61  SDLCSTNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSSPIQEPLTKSLGTSLKASDSMD 120
           S + S NG + +N        KTS ++S + S  +SK+SP + PL   + +++K    + 
Sbjct: 61  SPVTSPNGTTPSN--------KTSIVKSSMPS-SSSKASPAKSPLRNDMPSTVKDRSQLQ 120

Query: 121 SRPKNLVNAAVKEENVSIKPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTVSKNKVKKS 180
                      K+++   +   E+ DSEDD+PLS  L GN    +   VS  S    KK+
Sbjct: 121 -----------KDQS---ECKIEHEDSEDDRPLSSILSGNKGPTSSRQVS--SPQPEKKN 180

Query: 181 SGD-------------SDDEVPLSSKFQMKYASGVLGSKQHGFDEKKPLVPKVHQNGSTS 240
           +GD             SDDE P+SS F+ K  SG+ G  Q   DEKKPLV K+HQNGST 
Sbjct: 181 NGDRPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTV 240

Query: 241 RDKLQKPAVLSSKRPLPNADSHSPSQFSTKKPKLSDSSSPLSSK--------------LV 300
           ++++    VL  KRPL    + S  Q S KK K+S S + +  K              LV
Sbjct: 241 KNEVPNGKVL-GKRPLEK--NSSADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLV 300

Query: 301 S--LKAKQ-------EVDDDDDHIPISQRMKMVSATVN-------------KSSSSKPAV 360
           S  +KAKQ         DDDDD +PIS+R K  S+  N              SS++KP  
Sbjct: 301 SPKMKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKA 360

Query: 361 SKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQTHGV 420
             VVS   +   K +KK  K SKY  S+K  PS+GDGQKKWTTLVHNGVIFPPPY+ HG+
Sbjct: 361 RNVVSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGI 420

Query: 421 KMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHVIQNLDDC 480
           K+LYKGKPVDLT +QEEVATMFAVM++TDY  KP+F+ENFWNDWR+LLGK HVIQ LDDC
Sbjct: 421 KILYKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDC 480

Query: 481 DFTPIYDWYQMEKEKKKQMTTEEKKTLKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPGL 540
           DFTPIY+W+  EKEKKKQM+TEEKK LKEEK+KQEEKYMWA++DGVKEK+GNFRVEPPGL
Sbjct: 481 DFTPIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGL 540

Query: 541 FRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDP 600
           FRGRGEHPKMGKLK+RI P +IT+NIGKGAPIPECPI GERWKEV+HDNTVTWLAFW DP
Sbjct: 541 FRGRGEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADP 600

Query: 601 INQKEFKYVFLAASSSLKGQSDKEKYEKARMLKVN------AYTKDFTSKDPTRRQIAVA 660
           IN KEFKYVFL A SSLKG SDKEKYEKAR L  +       YTK+FT+KD   RQIAVA
Sbjct: 601 INPKEFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVA 660

Query: 661 TYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYENTVEVE 720
           TYLIDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE
Sbjct: 661 TYLIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVE 720

Query: 721 LPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNR 780
             V+KAI QFQ+GKS TDDLFD+LDT+KLNAHLK+L+PGLTAKVFRTYNASITLD+ML++
Sbjct: 721 PLVYKAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQ 780

Query: 781 GTKDGDVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDL 840
            TKDGDV++KIV YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL+++LKELK +L
Sbjct: 781 ETKDGDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNL 840

Query: 841 DRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKI 881
           DRAKKGKPPL+GSDGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKI
Sbjct: 841 DRAKKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKI 900

BLAST of PI0013838.1 vs. TAIR 10
Match: AT5G55300.3 (DNA topoisomerase I alpha )

HSP 1 Score: 429.9 bits (1104), Expect = 5.1e-120
Identity = 210/271 (77.49%), Postives = 246/271 (90.77%), Query Frame = 0

Query: 613 NEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRYENTVEVELPVFKAITQFQS 672
           ++ DDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE  V+KAI QFQ+
Sbjct: 291 DDDDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLVYKAIGQFQA 350

Query: 673 GKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDGDVSEKIV 732
           GKS TDDLFD+LDT+KLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TKDGDV++KIV
Sbjct: 351 GKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETKDGDVTQKIV 410

Query: 733 TYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILKELKIDLDRAKKGKPPLKG 792
            YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL+++LKELK +LDRAKKGKPPL+G
Sbjct: 411 VYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRAKKGKPPLEG 470

Query: 793 SDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAWC 852
           SDGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYLDPRITVAWC
Sbjct: 471 SDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYLDPRITVAWC 530

Query: 853 KRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 884
           KRHEVPIEKIF KSLL KFAWAMDV+P++RF
Sbjct: 531 KRHEVPIEKIFTKSLLEKFAWAMDVEPEYRF 561

HSP 2 Score: 99.8 bits (247), Expect = 1.2e-20
Identity = 108/308 (35.06%), Postives = 159/308 (51.62%), Query Frame = 0

Query: 1   MAVETFGKAPMFDD---DDEDNMPIVFKRSS--ASRQNQLDSEIKHSQAQRLNKLPGRQG 60
           M  ET  K P+ D+   D +D+ P+ FKR++  AS  NQ  S  + S+A    K+   + 
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMR- 60

Query: 61  SDLCSTNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSSPIQEPLTKSLGTSLKASDSMD 120
           S + S NG + +N        KTS ++S + S  +SK+SP + PL   + +++K    + 
Sbjct: 61  SPVTSPNGTTPSN--------KTSIVKSSMPS-SSSKASPAKSPLRNDMPSTVKDRSQLQ 120

Query: 121 SRPKNLVNAAVKEENVSIKPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTVSKNKVKKS 180
                      K+++   +   E+ DSEDD+PLS  L GN    +   VS  S    KK+
Sbjct: 121 -----------KDQS---ECKIEHEDSEDDRPLSSILSGNKGPTSSRQVS--SPQPEKKN 180

Query: 181 SGD-------------SDDEVPLSSKFQMKYASGVLGSKQHGFDEKKPLVPKVHQNGSTS 240
           +GD             SDDE P+SS F+ K  SG+ G  Q   DEKKPLV K+HQNGST 
Sbjct: 181 NGDRPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTV 240

Query: 241 RDKLQKPAVLSSKRPLPNADSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDH 291
           ++++    VL  KRPL    + S  Q S KK K+  S+SP S K+     K+E+ DD   
Sbjct: 241 KNEVPNGKVL-GKRPLEK--NSSADQSSLKKAKI--SASPTSVKMKQDSVKKEI-DDKGR 275

BLAST of PI0013838.1 vs. TAIR 10
Match: AT4G26701.1 (DNA binding;DNA topoisomerase type Is )

HSP 1 Score: 129.0 bits (323), Expect = 1.8e-29
Identity = 60/68 (88.24%), Postives = 64/68 (94.12%), Query Frame = 0

Query: 816 KIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAM 875
           KIEK+ERDM+TKEDLKTVALGTSKINY+DPRITVAWCKRHE PIEKIFNKSLL KFAWAM
Sbjct: 2   KIEKMERDMQTKEDLKTVALGTSKINYMDPRITVAWCKRHEAPIEKIFNKSLLEKFAWAM 61

Query: 876 DVDPDFRF 884
           DV+P F F
Sbjct: 62  DVEPHFTF 69