PI0005883.1 (mRNA) Melon (PI 482460) v1

Overview


Name	PI0005883.1
Type	mRNA
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Peptidase A1 domain-containing protein
Location	chr12: 14395963 .. 14397528 (-)
Sequence length	1566
RNA-Seq Expression	PI0005883.1
Synteny	PI0005883.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTTCAATATCATCAATCTCCATTGCAACAAAATTCTTGAGCCTATTCCTTCTTCTTGTACATATCTCAACACAAACCCTAGCTACAAACCCTAAAACCAATTTCCCCAAAGATTCTCTAGTTCTTGGTCTTGTTCATTCAAGAACATCCCTCCTTACACCCAAAAAAGGCTATAATTTCATTTCAAAGAAGAGAATGAAGGCAATGGATCAGATGGATGGTGATGATAATGTGATAGAGCCATTGAGAGAAATTAGGGATGGTTATTTAATGTCCTTAACAATAGGGACACCCCCACAAGTTGTTCAAGTGTATATGGACACTGGAAGTGATCTCACATGGGTTCCTTGTGGTAACCTTTCTTTTGATTGTCAAGATTGTGAGGAGTATCAAAACAATATTTCTGGCCCAAAATTGGCTGCCTTTTTGCCTACTCATTCTTCTACTTCTATTAGAGACACTTGTGGCAGTTCCTTTTGTATGGATATTCATAGCTCTGATAACCCTTTTGATCCTTGCACAATTGCTGGTTGTTCCTTAGCTACCCTTGTGAAGGGAACTTGCCCTAGACCTTGCCCTTCTTTTGCTTATACTTATGGAGCAAGCGGGGTTGTAACTGGAAGTTTAACAAGAGATGTTCTTTTTATGCATGGAAATTATCATAATAATAATGATGATAATAATAATAATAAGAAAATCCCTAGGTTTTGTTTTGGATGTGTCGGTGCAACTTATAGAGAGCCAATTGGGATTGCTGGTTTTGGTAGAGGCTTACTTTCTCTTCCTTTTCAATTAGGGTTTTCTCATAAGGGGTTTTCTCATTGTTTTTTACCTTTTAAATTCTCAAATAACCCTAATTTCTCAAGCCCTTTGATTCTTGGTAATCTTGCCATTTCTTCAAAAGATGAAAATTTGCAATTTACCCCTTTGTTGAAAAGTCCAATTTACCCTAACTATTACTATATTGGGCTTGAGTCAATTACCATTGGGAACGGAGATAATAATTTTAGATTTGGGGTTTCTTTTAAATTGAGAGAGATTGATACAAAGGGTAATGGGGGAATGTTGATTGATTCTGGTACTACTTATACTCATTTACCTGAACCATTGTATTCACAACTTATTTCTAATCTTGAATTAGTGATAAGTTATCCAAGAGCCAAACAAGTTGAACTCAATACTGGGTTTGATCTTTGTTATAAAGTTCCTTGTAAAAACAACAATTCTTCTTTTGTTGATGACTCTCAACTCCCTTCTATAACATTCCATTTTTTGAATAATGTTAGTGTTGTTTTGCCTCAAGGGAATAACTTCTATGCCATGGCTGCTCCAATTAACTCCACTGTGGTTAAATGTCTGTTGTATCAAAGCATGGACGGTGTCGGTGATGATAACGACAGTGACGACAACGGGCCAGCGGGCATTTTCGGAAGCTTTCAACAGCAAAATCTAGAGGTTGTTTATGATTTGGAGAAGGAAAGATTAGGGTTTCAACCAATGGATTGTGTTTCTGTTGCTGCCAATCAGGGACTTCACAAGAATGTGAGAAGGAATGAAAGTTGA

mRNA sequence

ATGACTTCAATATCATCAATCTCCATTGCAACAAAATTCTTGAGCCTATTCCTTCTTCTTGTACATATCTCAACACAAACCCTAGCTACAAACCCTAAAACCAATTTCCCCAAAGATTCTCTAGTTCTTGGTCTTGTTCATTCAAGAACATCCCTCCTTACACCCAAAAAAGGCTATAATTTCATTTCAAAGAAGAGAATGAAGGCAATGGATCAGATGGATGGTGATGATAATGTGATAGAGCCATTGAGAGAAATTAGGGATGGTTATTTAATGTCCTTAACAATAGGGACACCCCCACAAGTTGTTCAAGTGTATATGGACACTGGAAGTGATCTCACATGGGTTCCTTGTGGTAACCTTTCTTTTGATTGTCAAGATTGTGAGGAGTATCAAAACAATATTTCTGGCCCAAAATTGGCTGCCTTTTTGCCTACTCATTCTTCTACTTCTATTAGAGACACTTGTGGCAGTTCCTTTTGTATGGATATTCATAGCTCTGATAACCCTTTTGATCCTTGCACAATTGCTGGTTGTTCCTTAGCTACCCTTGTGAAGGGAACTTGCCCTAGACCTTGCCCTTCTTTTGCTTATACTTATGGAGCAAGCGGGGTTGTAACTGGAAGTTTAACAAGAGATGTTCTTTTTATGCATGGAAATTATCATAATAATAATGATGATAATAATAATAATAAGAAAATCCCTAGGTTTTGTTTTGGATGTGTCGGTGCAACTTATAGAGAGCCAATTGGGATTGCTGGTTTTGGTAGAGGCTTACTTTCTCTTCCTTTTCAATTAGGGTTTTCTCATAAGGGGTTTTCTCATTGTTTTTTACCTTTTAAATTCTCAAATAACCCTAATTTCTCAAGCCCTTTGATTCTTGGTAATCTTGCCATTTCTTCAAAAGATGAAAATTTGCAATTTACCCCTTTGTTGAAAAGTCCAATTTACCCTAACTATTACTATATTGGGCTTGAGTCAATTACCATTGGGAACGGAGATAATAATTTTAGATTTGGGGTTTCTTTTAAATTGAGAGAGATTGATACAAAGGGTAATGGGGGAATGTTGATTGATTCTGGTACTACTTATACTCATTTACCTGAACCATTGTATTCACAACTTATTTCTAATCTTGAATTAGTGATAAGTTATCCAAGAGCCAAACAAGTTGAACTCAATACTGGGTTTGATCTTTGTTATAAAGTTCCTTGTAAAAACAACAATTCTTCTTTTGTTGATGACTCTCAACTCCCTTCTATAACATTCCATTTTTTGAATAATGTTAGTGTTGTTTTGCCTCAAGGGAATAACTTCTATGCCATGGCTGCTCCAATTAACTCCACTGTGGTTAAATGTCTGTTGTATCAAAGCATGGACGGTGTCGGTGATGATAACGACAGTGACGACAACGGGCCAGCGGGCATTTTCGGAAGCTTTCAACAGCAAAATCTAGAGGTTGTTTATGATTTGGAGAAGGAAAGATTAGGGTTTCAACCAATGGATTGTGTTTCTGTTGCTGCCAATCAGGGACTTCACAAGAATGTGAGAAGGAATGAAAGTTGA

Coding sequence (CDS)

ATGACTTCAATATCATCAATCTCCATTGCAACAAAATTCTTGAGCCTATTCCTTCTTCTTGTACATATCTCAACACAAACCCTAGCTACAAACCCTAAAACCAATTTCCCCAAAGATTCTCTAGTTCTTGGTCTTGTTCATTCAAGAACATCCCTCCTTACACCCAAAAAAGGCTATAATTTCATTTCAAAGAAGAGAATGAAGGCAATGGATCAGATGGATGGTGATGATAATGTGATAGAGCCATTGAGAGAAATTAGGGATGGTTATTTAATGTCCTTAACAATAGGGACACCCCCACAAGTTGTTCAAGTGTATATGGACACTGGAAGTGATCTCACATGGGTTCCTTGTGGTAACCTTTCTTTTGATTGTCAAGATTGTGAGGAGTATCAAAACAATATTTCTGGCCCAAAATTGGCTGCCTTTTTGCCTACTCATTCTTCTACTTCTATTAGAGACACTTGTGGCAGTTCCTTTTGTATGGATATTCATAGCTCTGATAACCCTTTTGATCCTTGCACAATTGCTGGTTGTTCCTTAGCTACCCTTGTGAAGGGAACTTGCCCTAGACCTTGCCCTTCTTTTGCTTATACTTATGGAGCAAGCGGGGTTGTAACTGGAAGTTTAACAAGAGATGTTCTTTTTATGCATGGAAATTATCATAATAATAATGATGATAATAATAATAATAAGAAAATCCCTAGGTTTTGTTTTGGATGTGTCGGTGCAACTTATAGAGAGCCAATTGGGATTGCTGGTTTTGGTAGAGGCTTACTTTCTCTTCCTTTTCAATTAGGGTTTTCTCATAAGGGGTTTTCTCATTGTTTTTTACCTTTTAAATTCTCAAATAACCCTAATTTCTCAAGCCCTTTGATTCTTGGTAATCTTGCCATTTCTTCAAAAGATGAAAATTTGCAATTTACCCCTTTGTTGAAAAGTCCAATTTACCCTAACTATTACTATATTGGGCTTGAGTCAATTACCATTGGGAACGGAGATAATAATTTTAGATTTGGGGTTTCTTTTAAATTGAGAGAGATTGATACAAAGGGTAATGGGGGAATGTTGATTGATTCTGGTACTACTTATACTCATTTACCTGAACCATTGTATTCACAACTTATTTCTAATCTTGAATTAGTGATAAGTTATCCAAGAGCCAAACAAGTTGAACTCAATACTGGGTTTGATCTTTGTTATAAAGTTCCTTGTAAAAACAACAATTCTTCTTTTGTTGATGACTCTCAACTCCCTTCTATAACATTCCATTTTTTGAATAATGTTAGTGTTGTTTTGCCTCAAGGGAATAACTTCTATGCCATGGCTGCTCCAATTAACTCCACTGTGGTTAAATGTCTGTTGTATCAAAGCATGGACGGTGTCGGTGATGATAACGACAGTGACGACAACGGGCCAGCGGGCATTTTCGGAAGCTTTCAACAGCAAAATCTAGAGGTTGTTTATGATTTGGAGAAGGAAAGATTAGGGTTTCAACCAATGGATTGTGTTTCTGTTGCTGCCAATCAGGGACTTCACAAGAATGTGAGAAGGAATGAAAGTTGA

Protein sequence

MTSISSISIATKFLSLFLLLVHISTQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYNFISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLTIGTPPQVVQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNDDNNNNKKIPRFCFGCVGATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSGTTYTHLPEPLYSQLISNLELVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLPSITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQQNLEVVYDLEKERLGFQPMDCVSVAANQGLHKNVRRNES

Homology

[Close]

BLAST of PI0005883.1 vs. ExPASy Swiss-Prot
Match: Q940R4 (Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g16563 PE=2 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 5.8e-52
Identity = 148/450 (32.89%), Postives = 203/450 (45.11%), Query Frame = 0


Query: 90  YLMSLTIGTPPQVVQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNISGPKLAAFLPTHSS 149
           YL+SL++G+    V +Y+DTGSDL W PC    F C  CE      S P       + SS
Sbjct: 83  YLISLSVGSSSSAVSLYLDTGSDLVWFPC--RPFTCILCESKPLPPSPPS------SLSS 142

Query: 150 TSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKGTC---PRPCPSFAYTYGASGVV 209
           ++   +C S  C   HSS    D C I+ C L  +  G C     PCP F Y YG  G +
Sbjct: 143 SATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYG-DGSL 202

Query: 210 TGSLTRDVLFMHGNYHNNNDDNNNNKKIPRFCFGCVGATYREPIGIAGFGRGLLSLPFQL 269
              L  D L +             +  +  F FGC   T  EPIG+AGFGRG LSLP QL
Sbjct: 203 VAKLYSDSLSL------------PSVSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQL 262

Query: 270 GF--SHKG--FSHCFLPFKF-SNNPNFSSPLILGNLA------------------ISSKD 329
                H G  FS+C +   F S+     SPLILG                        K 
Sbjct: 263 AVHSPHLGNSFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKKK 322

Query: 330 ENLQFTPLLKSPIYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSGTT 389
               FT +L++P +P +Y + L+ I+IG  +          LR ID  G GG+++DSGTT
Sbjct: 323 NEFVFTEMLENPKHPYFYSVSLQGISIGKRN----IPAPAMLRRIDKNGGGGVVVDSGTT 382

Query: 390 YTHLPEPLYSQLISNLELVIS--YPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLPSI 449
           +T LP   Y+ ++   +  +   + RA +VE ++G   CY +             ++P++
Sbjct: 383 FTMLPAKFYNSVVEEFDSRVGRVHERADRVEPSSGMSPCYYLN---------QTVKVPAL 442

Query: 450 TFHFL-NNVSVVLPQGNNFYAMA----APINSTVVKCLLYQSMDGVGDDNDSDDNGPAGI 507
             HF  N  SV LP+ N FY              + CL+  +    G D      G   I
Sbjct: 443 VLHFAGNRSSVTLPRRNYFYEFMDGGDGKEEKRKIGCLMLMN----GGDESELRGGTGAI 494

[Close]

BLAST of PI0005883.1 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 4.2e-34
Identity = 128/445 (28.76%), Postives = 180/445 (40.45%), Query Frame = 0


Query: 64  KKRMKAMDQMDGDDNVIEPLREIRDG-YLMSLTIGTPPQVVQVYMDTGSDLTWVPCGNLS 123
           ++RM++++ M    + IE      DG YLM++ IGTP       MDTGSDL W  C    
Sbjct: 69  ERRMRSINAMLQSSSGIETPVYAGDGEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCE--- 128

Query: 124 FDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLA 183
             C  C      I       F P  SS+     C S +C D+     P + C    C   
Sbjct: 129 -PCTQCFSQPTPI-------FNPQDSSSFSTLPCESQYCQDL-----PSETCNNNEC--- 188

Query: 184 TLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNDDNNNNKKIPRFCFGC- 243
                        + Y YG      G +  +                    +P   FGC 
Sbjct: 189 ------------QYTYGYGDGSTTQGYMATETFTF------------ETSSVPNIAFGCG 248

Query: 244 ---VGATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLA 303
               G       G+ G G G LSLP QLG     FS+C   +  S+     S L LG+ A
Sbjct: 249 EDNQGFGQGNGAGLIGMGWGPLSLPSQLGVGQ--FSYCMTSYGSSS----PSTLALGS-A 308

Query: 304 ISSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLI 363
            S   E    T L+ S + P YYYI L+ IT+G GDN    G+     ++   G GGM+I
Sbjct: 309 ASGVPEGSPSTTLIHSSLNPTYYYITLQGITVG-GDN---LGIPSSTFQLQDDGTGGMII 368

Query: 364 DSGTTYTHLPEPLYSQLISNLELVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQL 423
           DSGTT T+LP+  Y+ +       I+ P     E ++G   C++ P   +        Q+
Sbjct: 369 DSGTTLTYLPQDAYNAVAQAFTDQINLPTVD--ESSSGLSTCFQQPSDGST------VQV 428

Query: 424 PSITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFG 483
           P I+  F   V  +  Q      + +P    +  CL   S   +G            IFG
Sbjct: 429 PEISMQFDGGVLNLGEQN----ILISPAEGVI--CLAMGSSSQLG----------ISIFG 435

Query: 484 SFQQQNLEVVYDLEKERLGFQPMDC 504
           + QQQ  +V+YDL+   + F P  C
Sbjct: 489 NIQQQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of PI0005883.1 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 2.5e-31
Identity = 123/437 (28.15%), Postives = 183/437 (41.88%), Query Frame = 0


Query: 78  NVIEPLREIRDGYLMSLTIGTPPQVVQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNISG 137
           +V+  L +    Y   L +GTP + V + +DTGSD+ W+ C      C+ C    + I  
Sbjct: 130 SVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCA----PCRRCYSQSDPI-- 189

Query: 138 PKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKGTCPRPCPSFA 197
                F P  S T     C S  C  + S          AGC+     + TC      + 
Sbjct: 190 -----FDPRKSKTYATIPCSSPHCRRLDS----------AGCNTR---RKTC-----LYQ 249

Query: 198 YTYGASGVVTGSLTRDVLFMHGNYHNNNDDNNNNKKIPRFCFGC--------VGATYREP 257
            +YG      G  + + L    N            ++     GC        VGA     
Sbjct: 250 VSYGDGSFTVGDFSTETLTFRRN------------RVKGVALGCGHDNEGLFVGAA---- 309

Query: 258 IGIAGFGRGLLSLPFQLG--FSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSKDENLQ 317
            G+ G G+G LS P Q G  F+ K FS+C +    S+ P   S ++ GN A+S      +
Sbjct: 310 -GLLGLGKGKLSFPGQTGHRFNQK-FSYCLVDRSASSKP---SSVVFGNAAVS---RIAR 369

Query: 318 FTPLLKSPIYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSGTTYTHL 377
           FTPLL +P    +YY+GL  I++G        GV+  L ++D  GNGG++IDSGT+ T L
Sbjct: 370 FTPLLSNPKLDTFYYVGLLGISVGGTRVP---GVTASLFKLDQIGNGGVIIDSGTSVTRL 429

Query: 378 PEPLYSQLISNLEL-VISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLPSITFHFL 437
             P Y  +     +   +  RA    L   FD C+ +       S +++ ++P++  HF 
Sbjct: 430 IRPAYIAMRDAFRVGAKTLKRAPDFSL---FDTCFDL-------SNMNEVKVPTVVLHF- 484

Query: 438 NNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQQNLE 497
               V LP  N       P+++    C  +           +   G   I G+ QQQ   
Sbjct: 490 RGADVSLPATN----YLIPVDTNGKFCFAF-----------AGTMGGLSIIGNIQQQGFR 484

Query: 498 VVYDLEKERLGFQPMDC 504
           VVYDL   R+GF P  C
Sbjct: 550 VVYDLASSRVGFAPGGC 484

[Close]

BLAST of PI0005883.1 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 1.3e-30
Identity = 129/447 (28.86%), Postives = 180/447 (40.27%), Query Frame = 0


Query: 65  KRMKAMDQMDGDDNVIEPLREIRDG-YLMSLTIGTPPQVVQVYMDTGSDLTWVPCGNLSF 124
           +R++ ++ M    + +E      DG YLM+L+IGTP Q     MDTGSDL W        
Sbjct: 69  RRLQRLEAMLNGPSGVETSVYAGDGEYLMNLSIGTPAQPFSAIMDTGSDLIWT------- 128

Query: 125 DCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLAT 184
            CQ C +  N         F P  SS+     C S  C  + S                 
Sbjct: 129 QCQPCTQCFNQ----STPIFNPQGSSSFSTLPCSSQLCQALSSP---------------- 188

Query: 185 LVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNDDNNNNKKIPRFCFGC-- 244
               TC      + Y YG      GS+  + L               +  IP   FGC  
Sbjct: 189 ----TCSNNFCQYTYGYGDGSETQGSMGTETLTF------------GSVSIPNITFGCGE 248

Query: 245 --VGATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAI 304
              G       G+ G GRG LSLP QL  +   FS+C  P   S   N    L+LG+LA 
Sbjct: 249 NNQGFGQGNGAGLVGMGRGPLSLPSQLDVTK--FSYCMTPIGSSTPSN----LLLGSLA- 308

Query: 305 SSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGDNNFRFGV---SFKLREIDTKGNGGM 364
           +S       T L++S   P +YYI L  +++G    + R  +   +F L      G GG+
Sbjct: 309 NSVTAGSPNTTLIQSSQIPTFYYITLNGLSVG----STRLPIDPSAFALN--SNNGTGGI 368

Query: 365 LIDSGTTYTHLPEPLYSQLISNLELVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDS 424
           +IDSGTT T+     Y  +       I+ P       ++GFDLC++ P   +N       
Sbjct: 369 IIDSGTTLTYFVNNAYQSVRQEFISQINLPVVN--GSSSGFDLCFQTPSDPSN------L 428

Query: 425 QLPSITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGI 484
           Q+P+   HF +   + LP  N F    +P N  +  CL   S            +    I
Sbjct: 429 QIPTFVMHF-DGGDLELPSENYF---ISPSNGLI--CLAMGS-----------SSQGMSI 434

Query: 485 FGSFQQQNLEVVYDLEKERLGFQPMDC 504
           FG+ QQQN+ VVYD     + F    C
Sbjct: 489 FGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of PI0005883.1 vs. ExPASy Swiss-Prot
Match: O04496 (Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 9.0e-29
Identity = 112/428 (26.17%), Postives = 183/428 (42.76%), Query Frame = 0


Query: 90  YLMSLTIGTPPQVVQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNISGPKLAAFLPTHSS 149
           Y++   +GTPPQ++ + +DT +D  W+PC      C  C                 +++S
Sbjct: 104 YVVRAKLGTPPQLMFMVLDTSNDAVWLPCSG----CSGC-----------------SNAS 163

Query: 150 TSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKGTCPRPCP-----SFAYTYGASG 209
           TS      S++             C+ A C+ A  +  TCP   P     SF  +YG   
Sbjct: 164 TSFNTNSSSTYST---------VSCSTAQCTQARGL--TCPSSSPQPSVCSFNQSYGGDS 223

Query: 210 VVTGSLTRDVLFMHGNYHNNNDDNNNNKKIPRFCFGCVGATYRE---PIGIAGFGRGLLS 269
             + SL +D L +  +             IP F FGC+ +       P G+ G GRG +S
Sbjct: 224 SFSASLVQDTLTLAPDV------------IPNFSFGCINSASGNSLPPQGLMGLGRGPMS 283

Query: 270 LPFQLGFSHKG-FSHCFLPFKFSNNPNFSSPLILGNLAISSKDENLQFTPLLKSPIYPNY 329
           L  Q    + G FS+C   F+   +  FS  L LG L    + +++++TPLL++P  P+ 
Sbjct: 284 LVSQTTSLYSGVFSYCLPSFR---SFYFSGSLKLGLL---GQPKSIRYTPLLRNPRRPSL 343

Query: 330 YYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSGTTYTHLPEPLYSQLISNLE 389
           YY+ L  +++G    + +  V       D     G +IDSGT  T   +P+Y  +     
Sbjct: 344 YYVNLTGVSVG----SVQVPVDPVYLTFDANSGAGTIIDSGTVITRFAQPVYEAIRDEFR 403

Query: 390 LVISYPRAKQVELNT-----GFDLCYKVPCKNNNSSFVDDSQLPSITFHFLNNVSVVLPQ 449
                   KQV +++      FD C+         S  +++  P IT H + ++ + LP 
Sbjct: 404 --------KQVNVSSFSTLGAFDTCF---------SADNENVAPKITLH-MTSLDLKLPM 448

Query: 450 GNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQQNLEVVYDLEKER 504
            N     +A      + CL   SM G+      + N    +  + QQQNL +++D+   R
Sbjct: 464 ENTLIHSSA----GTLTCL---SMAGI----RQNANAVLNVIANLQQQNLRILFDVPNSR 448

[Close]

BLAST of PI0005883.1 vs. ExPASy TrEMBL
Match: A0A0A0LYP0 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G704590 PE=3 SV=1)

HSP 1 Score: 1034.6 bits (2674), Expect = 1.4e-298
Identity = 503/521 (96.55%), Postives = 512/521 (98.27%), Query Frame = 0


Query: 1   MTSISSISIATKFLSLFLLLVHISTQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60
           M SISSIS ATKFLSLFLLLVH+STQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN
Sbjct: 1   MPSISSISTATKFLSLFLLLVHVSTQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60

Query: 61  FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLTIGTPPQVVQVYMDTGSDLTWVPCGN 120
           FISKKRMKAMDQ DGDDNVIEPLREIRDGYLMSL+IGTPPQVVQVYMDTGSDLTWVPCGN
Sbjct: 61  FISKKRMKAMDQTDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDCEEYQNNISGP+LAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCEEYQNNISGPRLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180

Query: 181 LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNDDNNNNKKIPRFCFG 240
           LA+LVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLF HGNY+NN   NNNNK+IPRFCFG
Sbjct: 181 LASLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFTHGNYNNN---NNNNKQIPRFCFG 240

Query: 241 CVGATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAIS 300
           CVGATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAIS
Sbjct: 241 CVGATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAIS 300

Query: 301 SKDENLQFTPLLKSPIYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDS 360
           SKDENLQFTPLLKSP+YPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDS
Sbjct: 301 SKDENLQFTPLLKSPMYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDS 360

Query: 361 GTTYTHLPEPLYSQLISNLELVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLPS 420
           GTTYTHLPEPLYSQLISNLELVI YPRAKQVELNTGFDLCYKVPCKNNNSSFVDD+QLPS
Sbjct: 361 GTTYTHLPEPLYSQLISNLELVIGYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDAQLPS 420

Query: 421 ITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSF 480
           ITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSF
Sbjct: 421 ITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSF 480

Query: 481 QQQNLEVVYDLEKERLGFQPMDCVSVAANQGLHKNVRRNES 522
           QQQN+EVVYDLEKERLGFQPMDCVSVAA QGLHKNVRRNES
Sbjct: 481 QQQNIEVVYDLEKERLGFQPMDCVSVAAKQGLHKNVRRNES 518

[Close]

BLAST of PI0005883.1 vs. ExPASy TrEMBL
Match: A0A5A7TNC9 (Aspartic proteinase nepenthesin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold129G00970 PE=3 SV=1)

HSP 1 Score: 1033.9 bits (2672), Expect = 2.3e-298
Identity = 504/520 (96.92%), Postives = 512/520 (98.46%), Query Frame = 0


Query: 1   MTSISSISIATKFLSLFLLLVHISTQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60
           M SISS SIATKFLSLFLLLVH S QTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN
Sbjct: 1   MPSISSTSIATKFLSLFLLLVHASKQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60

Query: 61  FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLTIGTPPQVVQVYMDTGSDLTWVPCGN 120
           FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSL+IGTPPQVVQVYMDTGSDLTWVPCGN
Sbjct: 61  FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180

Query: 181 LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNDDN-NNNKKIPRFCF 240
           LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNN++N NNNK++PRFCF
Sbjct: 181 LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCF 240

Query: 241 GCVGATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAI 300
           GCVGATYREPIGIAGFGRGLLSLPFQLGFS KGFSHCFLPFKFSNNPNFSSPLILG+LAI
Sbjct: 241 GCVGATYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHLAI 300

Query: 301 SSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLID 360
           SSKDENLQFTPLLKSPIYPNYYYIGLESITIGNG+NNFRFGVSFKLREIDTKGNGGMLID
Sbjct: 301 SSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLID 360

Query: 361 SGTTYTHLPEPLYSQLISNLELVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLP 420
           SGTTYTHLPEPLYSQLISNLE VISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLP
Sbjct: 361 SGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLP 420

Query: 421 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 480
           SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS
Sbjct: 421 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 480

Query: 481 FQQQNLEVVYDLEKERLGFQPMDCVSVAANQGLHKNVRRN 520
           FQQQNL+VVYDLEKERLGFQ MDCVSVAANQGLHKNVRRN
Sbjct: 481 FQQQNLQVVYDLEKERLGFQAMDCVSVAANQGLHKNVRRN 520

[Close]

BLAST of PI0005883.1 vs. ExPASy TrEMBL
Match: A0A1S3CAK9 (aspartic proteinase nepenthesin-2 OS=Cucumis melo OX=3656 GN=LOC103498305 PE=3 SV=1)

HSP 1 Score: 1033.9 bits (2672), Expect = 2.3e-298
Identity = 504/520 (96.92%), Postives = 512/520 (98.46%), Query Frame = 0


Query: 1   MTSISSISIATKFLSLFLLLVHISTQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60
           M SISS SIATKFLSLFLLLVH S QTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN
Sbjct: 1   MPSISSTSIATKFLSLFLLLVHASKQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60

Query: 61  FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLTIGTPPQVVQVYMDTGSDLTWVPCGN 120
           FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSL+IGTPPQVVQVYMDTGSDLTWVPCGN
Sbjct: 61  FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180

Query: 181 LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNDDN-NNNKKIPRFCF 240
           LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNN++N NNNK++PRFCF
Sbjct: 181 LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCF 240

Query: 241 GCVGATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAI 300
           GCVGATYREPIGIAGFGRGLLSLPFQLGFS KGFSHCFLPFKFSNNPNFSSPLILG+LAI
Sbjct: 241 GCVGATYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHLAI 300

Query: 301 SSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLID 360
           SSKDENLQFTPLLKSPIYPNYYYIGLESITIGNG+NNFRFGVSFKLREIDTKGNGGMLID
Sbjct: 301 SSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLID 360

Query: 361 SGTTYTHLPEPLYSQLISNLELVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLP 420
           SGTTYTHLPEPLYSQLISNLE VISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLP
Sbjct: 361 SGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLP 420

Query: 421 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 480
           SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS
Sbjct: 421 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 480

Query: 481 FQQQNLEVVYDLEKERLGFQPMDCVSVAANQGLHKNVRRN 520
           FQQQNL+VVYDLEKERLGFQ MDCVSVAANQGLHKNVRRN
Sbjct: 481 FQQQNLQVVYDLEKERLGFQAMDCVSVAANQGLHKNVRRN 520

[Close]

BLAST of PI0005883.1 vs. ExPASy TrEMBL
Match: A0A6J1CMP8 (probable aspartyl protease At4g16563 OS=Momordica charantia OX=3673 GN=LOC111012684 PE=3 SV=1)

HSP 1 Score: 835.9 bits (2158), Expect = 9.4e-239
Identity = 420/529 (79.40%), Postives = 461/529 (87.15%), Query Frame = 0


Query: 2   TSISSISIATKFLSLFLLLVHI--STQTLATNPKTNFPK-DSLVLGLVHSRTSLLTPKKG 61
           T  S+ +I++K L+ FLLL+ +   ++T A   + NFP  DSLVLGLVHSRTSLLTPK+G
Sbjct: 4   TKSSATTISSKVLTFFLLLILLLSVSETAARPHRNNFPNTDSLVLGLVHSRTSLLTPKRG 63

Query: 62  Y---NFISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLTIGTPPQVVQVYMDTGSDLTW 121
           Y      S    K M+++ G DNVIEPLREIRDGYL+SLT+GTPPQV+QVYMDTGSDLTW
Sbjct: 64  YFSRKGSSSSINKPMEEI-GSDNVIEPLREIRDGYLISLTLGTPPQVIQVYMDTGSDLTW 123

Query: 122 VPCGNLSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCT 181
           VPCGNLSFDCQDCEEYQNN+SGPKLA F PTHSSTSIRDTCGSSFCMDIHSSDNPFDPCT
Sbjct: 124 VPCGNLSFDCQDCEEYQNNVSGPKLAPFSPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCT 183

Query: 182 IAGCSLATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNDDNNNNKKIP 241
           IAGCSLATLVKGTCPRPCPSFAYTYGASGVVTG+LT+DV+ MHG         N+  +IP
Sbjct: 184 IAGCSLATLVKGTCPRPCPSFAYTYGASGVVTGTLTKDVILMHG------VSPNSTTQIP 243

Query: 242 RFCFGCVGATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILG 301
           RFCFGCVGATYREPIGIAGFGRGLLSLP QLGFSHKGFSHCFLPFKFSNNPNFSSPLILG
Sbjct: 244 RFCFGCVGATYREPIGIAGFGRGLLSLPSQLGFSHKGFSHCFLPFKFSNNPNFSSPLILG 303

Query: 302 NLAISSKDENLQFTPLLKSPIYPNYYYIGLESITIGN--GDNNFRFGVSFKLREIDTKGN 361
           +LAISSKD +LQFTPLLKSP+YPNYYYIGLES+TIG+  G+NN RFGVS KLREIDTKGN
Sbjct: 304 SLAISSKDHSLQFTPLLKSPLYPNYYYIGLESVTIGDGIGNNNSRFGVSLKLREIDTKGN 363

Query: 362 GGMLIDSGTTYTHLPEPLYSQLISNLELVISYPRAKQVELNTGFDLCYKVPCKNNN--SS 421
           GGMLIDSGTTYTHLPEPLYSQLISNLE V++YPRAKQVE+NTGFDLCYK+PCKNN   SS
Sbjct: 364 GGMLIDSGTTYTHLPEPLYSQLISNLESVVTYPRAKQVEINTGFDLCYKIPCKNNTNFSS 423

Query: 422 FVDD---SQLPSITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDND- 481
            +DD   + LPSITFHFLNNVSVVLPQGNNFYAMAAP NSTVVKCLL+QSMDG G D D 
Sbjct: 424 SMDDYCSNLLPSITFHFLNNVSVVLPQGNNFYAMAAPTNSTVVKCLLFQSMDGGGGDGDG 483

Query: 482 -SDDNGPAGIFGSFQQQNLEVVYDLEKERLGFQPMDCVSVAANQGLHKN 516
             DD+GPAGIFGSFQQQN+EVVYDL+KER+GFQ MDC S AA+QGLHKN
Sbjct: 484 NGDDDGPAGIFGSFQQQNMEVVYDLQKERIGFQTMDCASSAASQGLHKN 525

[Close]

BLAST of PI0005883.1 vs. ExPASy TrEMBL
Match: A0A6J1EHM1 (probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC111434252 PE=3 SV=1)

HSP 1 Score: 831.2 bits (2146), Expect = 2.3e-237
Identity = 415/514 (80.74%), Postives = 453/514 (88.13%), Query Frame = 0


Query: 1   MTSISSISIATKFLSLFLLLVHISTQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60
           M SI++ S     L L L+      QTLA NPKT F KDSLVLGLVHSRTSLLTPK+GYN
Sbjct: 1   MASIAARSYFVLVLVLVLVSGEAMGQTLA-NPKTKFLKDSLVLGLVHSRTSLLTPKRGYN 60

Query: 61  FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLTIGTPPQVVQVYMDTGSDLTWVPCGN 120
            +  KR+K M+   G+D+VIEPLREIRDGYLMSLT+GTPPQV+QVYMDTGSDLTWVPCGN
Sbjct: 61  SLLTKRIKPMEM--GNDDVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDC+EYQNN+ GPKLAAFLPTHSSTSIRDTCGSSFC+DIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCDEYQNNVLGPKLAAFLPTHSSTSIRDTCGSSFCIDIHSSDNPFDPCTIAGCS 180

Query: 181 LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNDDNNNNKKIPRFCFG 240
           LATLVKGTCPRPCPSF+YTYGASG+V G+LT+DV+F+HGN        N+++KIP+FCFG
Sbjct: 181 LATLVKGTCPRPCPSFSYTYGASGLVIGTLTKDVIFIHGN------SPNSSRKIPKFCFG 240

Query: 241 CVGATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAIS 300
           CVGATYREPIGIAGFGRGLLSLP QLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAIS
Sbjct: 241 CVGATYREPIGIAGFGRGLLSLPSQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAIS 300

Query: 301 SKDENLQFTPLLKSPIYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDS 360
           SK E+L+FTP LKSP YPNYYYIGLESITIGNG+N  RFGVS +LREIDTKGNGG+LIDS
Sbjct: 301 SK-EHLKFTPFLKSPFYPNYYYIGLESITIGNGENYSRFGVSLQLREIDTKGNGGILIDS 360

Query: 361 GTTYTHLPEPLYSQLISNLELVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLPS 420
           GTTYTHLPEPLYSQLISNLE +ISYPRAK+ ELNTGFDLCYKVP K NN+ F D+ +LPS
Sbjct: 361 GTTYTHLPEPLYSQLISNLESLISYPRAKEHELNTGFDLCYKVPYK-NNTFFSDEFELPS 420

Query: 421 ITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSF 480
           ITFHFLNNVSVVLPQGN+FYAMAAP NSTVVKCLL+QSMDG GD       GPAGIFGSF
Sbjct: 421 ITFHFLNNVSVVLPQGNSFYAMAAPSNSTVVKCLLFQSMDGDGD-------GPAGIFGSF 480

Query: 481 QQQNLEVVYDLEKERLGFQPMDCVSVAANQGLHK 515
           QQQNLEVVYDLEKERLGF+ MDC SVA +QGLHK
Sbjct: 481 QQQNLEVVYDLEKERLGFEAMDCASVAVSQGLHK 496

[Close]

BLAST of PI0005883.1 vs. NCBI nr
Match: XP_004145478.2 (probable aspartyl protease At4g16563 [Cucumis sativus] >KGN66888.1 hypothetical protein Csa_007266 [Cucumis sativus])

HSP 1 Score: 1034.6 bits (2674), Expect = 2.8e-298
Identity = 503/521 (96.55%), Postives = 512/521 (98.27%), Query Frame = 0


Query: 1   MTSISSISIATKFLSLFLLLVHISTQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60
           M SISSIS ATKFLSLFLLLVH+STQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN
Sbjct: 1   MPSISSISTATKFLSLFLLLVHVSTQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60

Query: 61  FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLTIGTPPQVVQVYMDTGSDLTWVPCGN 120
           FISKKRMKAMDQ DGDDNVIEPLREIRDGYLMSL+IGTPPQVVQVYMDTGSDLTWVPCGN
Sbjct: 61  FISKKRMKAMDQTDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDCEEYQNNISGP+LAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCEEYQNNISGPRLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180

Query: 181 LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNDDNNNNKKIPRFCFG 240
           LA+LVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLF HGNY+NN   NNNNK+IPRFCFG
Sbjct: 181 LASLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFTHGNYNNN---NNNNKQIPRFCFG 240

Query: 241 CVGATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAIS 300
           CVGATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAIS
Sbjct: 241 CVGATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAIS 300

Query: 301 SKDENLQFTPLLKSPIYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDS 360
           SKDENLQFTPLLKSP+YPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDS
Sbjct: 301 SKDENLQFTPLLKSPMYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDS 360

Query: 361 GTTYTHLPEPLYSQLISNLELVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLPS 420
           GTTYTHLPEPLYSQLISNLELVI YPRAKQVELNTGFDLCYKVPCKNNNSSFVDD+QLPS
Sbjct: 361 GTTYTHLPEPLYSQLISNLELVIGYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDAQLPS 420

Query: 421 ITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSF 480
           ITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSF
Sbjct: 421 ITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSF 480

Query: 481 QQQNLEVVYDLEKERLGFQPMDCVSVAANQGLHKNVRRNES 522
           QQQN+EVVYDLEKERLGFQPMDCVSVAA QGLHKNVRRNES
Sbjct: 481 QQQNIEVVYDLEKERLGFQPMDCVSVAAKQGLHKNVRRNES 518

[Close]

BLAST of PI0005883.1 vs. NCBI nr
Match: XP_008459091.1 (PREDICTED: aspartic proteinase nepenthesin-2 [Cucumis melo] >KAA0043307.1 aspartic proteinase nepenthesin-2 [Cucumis melo var. makuwa] >TYK29371.1 aspartic proteinase nepenthesin-2 [Cucumis melo var. makuwa])

HSP 1 Score: 1033.9 bits (2672), Expect = 4.8e-298
Identity = 504/520 (96.92%), Postives = 512/520 (98.46%), Query Frame = 0


Query: 1   MTSISSISIATKFLSLFLLLVHISTQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60
           M SISS SIATKFLSLFLLLVH S QTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN
Sbjct: 1   MPSISSTSIATKFLSLFLLLVHASKQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60

Query: 61  FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLTIGTPPQVVQVYMDTGSDLTWVPCGN 120
           FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSL+IGTPPQVVQVYMDTGSDLTWVPCGN
Sbjct: 61  FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180

Query: 181 LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNDDN-NNNKKIPRFCF 240
           LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNN++N NNNK++PRFCF
Sbjct: 181 LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCF 240

Query: 241 GCVGATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAI 300
           GCVGATYREPIGIAGFGRGLLSLPFQLGFS KGFSHCFLPFKFSNNPNFSSPLILG+LAI
Sbjct: 241 GCVGATYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHLAI 300

Query: 301 SSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLID 360
           SSKDENLQFTPLLKSPIYPNYYYIGLESITIGNG+NNFRFGVSFKLREIDTKGNGGMLID
Sbjct: 301 SSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLID 360

Query: 361 SGTTYTHLPEPLYSQLISNLELVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLP 420
           SGTTYTHLPEPLYSQLISNLE VISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLP
Sbjct: 361 SGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLP 420

Query: 421 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 480
           SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS
Sbjct: 421 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 480

Query: 481 FQQQNLEVVYDLEKERLGFQPMDCVSVAANQGLHKNVRRN 520
           FQQQNL+VVYDLEKERLGFQ MDCVSVAANQGLHKNVRRN
Sbjct: 481 FQQQNLQVVYDLEKERLGFQAMDCVSVAANQGLHKNVRRN 520

[Close]

BLAST of PI0005883.1 vs. NCBI nr
Match: XP_038893627.1 (probable aspartyl protease At4g16563 [Benincasa hispida])

HSP 1 Score: 938.7 bits (2425), Expect = 2.1e-269
Identity = 460/516 (89.15%), Postives = 482/516 (93.41%), Query Frame = 0


Query: 1   MTSISSISIATKFLSLFLLLVHISTQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60
           M SIS+ S A K LS FLLLV++S +TLATNPKTN PKDSLV+GLVHSRT+LLTPKKGYN
Sbjct: 1   MASIST-SFAKKILSYFLLLVYVSRKTLATNPKTNGPKDSLVIGLVHSRTTLLTPKKGYN 60

Query: 61  FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLTIGTPPQVVQVYMDTGSDLTWVPCGN 120
           FIS+KRMKAM+    DDNVIEPLREIRDGYLMSLT+GTPPQV+QVYMDTGSDLTWVPCGN
Sbjct: 61  FISRKRMKAMEM---DDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDCEEYQNN+SGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCEEYQNNVSGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180

Query: 181 LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNDDNNNNKKIPRFCFG 240
           LATLVK TCPRPCPSFAYTYGASGVV G+LTRDVL MH N  N N  N++ KK PRFCFG
Sbjct: 181 LATLVKATCPRPCPSFAYTYGASGVVIGTLTRDVLLMHIN--NINSPNSSTKKTPRFCFG 240

Query: 241 CVGATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAIS 300
           CVGA+YREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLA+S
Sbjct: 241 CVGASYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAVS 300

Query: 301 SKDENLQFTPLLKSPIYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDS 360
           SKDE+LQFTPLLKSPIYPNYYYIGLESITIGNG++NFRFGVSF LREIDTKGNGGMLIDS
Sbjct: 301 SKDEHLQFTPLLKSPIYPNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDS 360

Query: 361 GTTYTHLPEPLYSQLISNLELVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLPS 420
           GTTYTHLPEPLYSQLISNLE VISYPRAKQVELNTGFDLCYKVPCKNNN SF+DDSQLPS
Sbjct: 361 GTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPS 420

Query: 421 ITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSF 480
           ITFHFLNNVSVVLPQ NNFYAMAAPINSTVVKCLL+QSMDGVG D D D +GPAGIFGSF
Sbjct: 421 ITFHFLNNVSVVLPQENNFYAMAAPINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFGSF 480

Query: 481 QQQNLEVVYDLEKERLGFQPMDCVSVAANQGLHKNV 517
           QQQNLEVVYDLEKERLGFQPMDC  VAA QGLHKNV
Sbjct: 481 QQQNLEVVYDLEKERLGFQPMDCAYVAATQGLHKNV 510

[Close]

BLAST of PI0005883.1 vs. NCBI nr
Match: XP_023520027.1 (probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 839.7 bits (2168), Expect = 1.3e-239
Identity = 417/510 (81.76%), Postives = 457/510 (89.61%), Query Frame = 0


Query: 9   IATKFLSLFLLLVHIS----TQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYNFISK 68
           +A  F  L L+LV +S     QTLA NPKT F KDSLVLGLVHSRTSLLTPK+GYN +S+
Sbjct: 5   VAKSFFVLVLVLVLVSGEAMGQTLA-NPKTKFLKDSLVLGLVHSRTSLLTPKRGYNSLSR 64

Query: 69  KRMKAMDQMDGDDNVIEPLREIRDGYLMSLTIGTPPQVVQVYMDTGSDLTWVPCGNLSFD 128
           KR+K M+   G+D+VIEPLREIRDGYLMSLT+GTPPQV+QVYMDTGSDLTWVPCGNLSFD
Sbjct: 65  KRIKPMEM--GNDDVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFD 124

Query: 129 CQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATL 188
           CQDC+EYQNN+ GPKLAAFLPTHSSTSIRDTCGSSFC+DIHSSDNPFDPCTIAGCSLATL
Sbjct: 125 CQDCDEYQNNVLGPKLAAFLPTHSSTSIRDTCGSSFCIDIHSSDNPFDPCTIAGCSLATL 184

Query: 189 VKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNDDNNNNKKIPRFCFGCVGA 248
           VKGTCPRPCPSF+YTYGASG+V G+LT+DV+F+HGN        N+++KIP+FCFGCVGA
Sbjct: 185 VKGTCPRPCPSFSYTYGASGLVIGTLTKDVIFIHGN------SPNSSRKIPKFCFGCVGA 244

Query: 249 TYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSKDE 308
           TYREPIGIAGFGRGLLSLP+QLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSK E
Sbjct: 245 TYREPIGIAGFGRGLLSLPYQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSK-E 304

Query: 309 NLQFTPLLKSPIYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSGTTY 368
           +L+FTPLLKSP YPNYYYIGLESITIGNG+N  RFGVS +LREIDTKGNGG+LIDSGTTY
Sbjct: 305 HLKFTPLLKSPFYPNYYYIGLESITIGNGENYSRFGVSLQLREIDTKGNGGILIDSGTTY 364

Query: 369 THLPEPLYSQLISNLELVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLPSITFH 428
           THLPEPLYSQLISNLE +ISYPRAK+ ELNTGFDLCYKVP K NN+ F D+ +LPSITFH
Sbjct: 365 THLPEPLYSQLISNLESLISYPRAKEHELNTGFDLCYKVPYK-NNTFFSDEFELPSITFH 424

Query: 429 FLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQQN 488
           FLNNVSVVLPQGN+FYAMAAP NSTVVKCLL+QSMDG GD       GPAGIFGSFQQQN
Sbjct: 425 FLNNVSVVLPQGNSFYAMAAPSNSTVVKCLLFQSMDGDGD-------GPAGIFGSFQQQN 484

Query: 489 LEVVYDLEKERLGFQPMDCVSVAANQGLHK 515
           LEVVYDLEKERLGF+ MDC SVA +QGLHK
Sbjct: 485 LEVVYDLEKERLGFEGMDCASVAVSQGLHK 496

[Close]

BLAST of PI0005883.1 vs. NCBI nr
Match: XP_022142611.1 (probable aspartyl protease At4g16563 [Momordica charantia])

HSP 1 Score: 835.9 bits (2158), Expect = 1.9e-238
Identity = 420/529 (79.40%), Postives = 461/529 (87.15%), Query Frame = 0


Query: 2   TSISSISIATKFLSLFLLLVHI--STQTLATNPKTNFPK-DSLVLGLVHSRTSLLTPKKG 61
           T  S+ +I++K L+ FLLL+ +   ++T A   + NFP  DSLVLGLVHSRTSLLTPK+G
Sbjct: 4   TKSSATTISSKVLTFFLLLILLLSVSETAARPHRNNFPNTDSLVLGLVHSRTSLLTPKRG 63

Query: 62  Y---NFISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLTIGTPPQVVQVYMDTGSDLTW 121
           Y      S    K M+++ G DNVIEPLREIRDGYL+SLT+GTPPQV+QVYMDTGSDLTW
Sbjct: 64  YFSRKGSSSSINKPMEEI-GSDNVIEPLREIRDGYLISLTLGTPPQVIQVYMDTGSDLTW 123

Query: 122 VPCGNLSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCT 181
           VPCGNLSFDCQDCEEYQNN+SGPKLA F PTHSSTSIRDTCGSSFCMDIHSSDNPFDPCT
Sbjct: 124 VPCGNLSFDCQDCEEYQNNVSGPKLAPFSPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCT 183

Query: 182 IAGCSLATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNDDNNNNKKIP 241
           IAGCSLATLVKGTCPRPCPSFAYTYGASGVVTG+LT+DV+ MHG         N+  +IP
Sbjct: 184 IAGCSLATLVKGTCPRPCPSFAYTYGASGVVTGTLTKDVILMHG------VSPNSTTQIP 243

Query: 242 RFCFGCVGATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILG 301
           RFCFGCVGATYREPIGIAGFGRGLLSLP QLGFSHKGFSHCFLPFKFSNNPNFSSPLILG
Sbjct: 244 RFCFGCVGATYREPIGIAGFGRGLLSLPSQLGFSHKGFSHCFLPFKFSNNPNFSSPLILG 303

Query: 302 NLAISSKDENLQFTPLLKSPIYPNYYYIGLESITIGN--GDNNFRFGVSFKLREIDTKGN 361
           +LAISSKD +LQFTPLLKSP+YPNYYYIGLES+TIG+  G+NN RFGVS KLREIDTKGN
Sbjct: 304 SLAISSKDHSLQFTPLLKSPLYPNYYYIGLESVTIGDGIGNNNSRFGVSLKLREIDTKGN 363

Query: 362 GGMLIDSGTTYTHLPEPLYSQLISNLELVISYPRAKQVELNTGFDLCYKVPCKNNN--SS 421
           GGMLIDSGTTYTHLPEPLYSQLISNLE V++YPRAKQVE+NTGFDLCYK+PCKNN   SS
Sbjct: 364 GGMLIDSGTTYTHLPEPLYSQLISNLESVVTYPRAKQVEINTGFDLCYKIPCKNNTNFSS 423

Query: 422 FVDD---SQLPSITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDND- 481
            +DD   + LPSITFHFLNNVSVVLPQGNNFYAMAAP NSTVVKCLL+QSMDG G D D 
Sbjct: 424 SMDDYCSNLLPSITFHFLNNVSVVLPQGNNFYAMAAPTNSTVVKCLLFQSMDGGGGDGDG 483

Query: 482 -SDDNGPAGIFGSFQQQNLEVVYDLEKERLGFQPMDCVSVAANQGLHKN 516
             DD+GPAGIFGSFQQQN+EVVYDL+KER+GFQ MDC S AA+QGLHKN
Sbjct: 484 NGDDDGPAGIFGSFQQQNMEVVYDLQKERIGFQTMDCASSAASQGLHKN 525

[Close]

BLAST of PI0005883.1 vs. TAIR 10
Match: AT5G45120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 590.5 bits (1521), Expect = 1.3e-168
Identity = 304/514 (59.14%), Postives = 374/514 (72.76%), Query Frame = 0


Query: 11  TKFLSLFL---LLVHISTQTLATNPKTNFPKDS--LVLGLVHSRTSLLTPKKGYNFISKK 70
           T  L LFL   LL++ + +T A   K      S  LVL L  S  SL TPK       KK
Sbjct: 5   THVLFLFLLITLLLNTTNKTQARQHKNPSSSSSSFLVLTLTKSSVSLPTPKSQTQERIKK 64

Query: 71  RMKAMDQMDGDDNVIEPLREIRDGYLMSLTIGTPPQVVQVYMDTGSDLTWVPCGNLSFDC 130
            + ++D       V+EPLRE+RDGYL++L IGTPPQ VQVY+DTGSDLTWVPCGNLSFDC
Sbjct: 65  PLSSVDV------VMEPLREVRDGYLITLNIGTPPQAVQVYLDTGSDLTWVPCGNLSFDC 124

Query: 131 QDCEEYQNN-ISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATL 190
            +C + +NN +  P  + F P HSSTS RD+C SSFC++IHSSDNPFDPC +AGCS++ L
Sbjct: 125 IECYDLKNNDLKSP--SVFSPLHSSTSFRDSCASSFCVEIHSSDNPFDPCAVAGCSVSML 184

Query: 191 VKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNDDNNNNKKIPRFCFGCVGA 250
           +K TC RPCPSFAYTYG  G+++G LTRD+L                + +PRF FGCV +
Sbjct: 185 LKSTCVRPCPSFAYTYGEGGLISGILTRDIL------------KARTRDVPRFSFGCVTS 244

Query: 251 TYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAIS-SKD 310
           TYREPIGIAGFGRGLLSLP QLGF  KGFSHCFLPFKF NNPN SSPLILG  A+S +  
Sbjct: 245 TYREPIGIAGFGRGLLSLPSQLGFLEKGFSHCFLPFKFVNNPNISSPLILGASALSINLT 304

Query: 311 ENLQFTPLLKSPIYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSGTT 370
           ++LQFTP+L +P+YPN YYIGLESITIG   N     V   LR+ D++GNGGML+DSGTT
Sbjct: 305 DSLQFTPMLNTPMYPNSYYIGLESITIGT--NITPTQVPLTLRQFDSQGNGGMLVDSGTT 364

Query: 371 YTHLPEPLYSQLISNLELVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQL---PS 430
           YTHLPEP YSQL++ L+  I+YPRA + E  TGFDLCYKVPC NNN + +++  +   PS
Sbjct: 365 YTHLPEPFYSQLLTTLQSTITYPRATETESRTGFDLCYKVPCPNNNLTSLENDVMMIFPS 424

Query: 431 ITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSF 490
           ITFHFLNN +++LPQGN+FYAM+AP + +VV+CLL+Q+M       +  D GPAG+FGSF
Sbjct: 425 ITFHFLNNATLLLPQGNSFYAMSAPSDGSVVQCLLFQNM-------EDGDYGPAGVFGSF 484

Query: 491 QQQNLEVVYDLEKERLGFQPMDCVSVAANQGLHK 515
           QQQN++VVYDLEKER+GFQ MDCV  AA+ GL++
Sbjct: 485 QQQNVKVVYDLEKERIGFQAMDCVLEAASHGLNQ 489

[Close]

BLAST of PI0005883.1 vs. TAIR 10
Match: AT4G16563.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 206.8 bits (525), Expect = 4.1e-53
Identity = 148/450 (32.89%), Postives = 203/450 (45.11%), Query Frame = 0


Query: 90  YLMSLTIGTPPQVVQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNISGPKLAAFLPTHSS 149
           YL+SL++G+    V +Y+DTGSDL W PC    F C  CE      S P       + SS
Sbjct: 83  YLISLSVGSSSSAVSLYLDTGSDLVWFPC--RPFTCILCESKPLPPSPPS------SLSS 142

Query: 150 TSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKGTC---PRPCPSFAYTYGASGVV 209
           ++   +C S  C   HSS    D C I+ C L  +  G C     PCP F Y YG  G +
Sbjct: 143 SATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYG-DGSL 202

Query: 210 TGSLTRDVLFMHGNYHNNNDDNNNNKKIPRFCFGCVGATYREPIGIAGFGRGLLSLPFQL 269
              L  D L +             +  +  F FGC   T  EPIG+AGFGRG LSLP QL
Sbjct: 203 VAKLYSDSLSL------------PSVSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQL 262

Query: 270 GF--SHKG--FSHCFLPFKF-SNNPNFSSPLILGNLA------------------ISSKD 329
                H G  FS+C +   F S+     SPLILG                        K 
Sbjct: 263 AVHSPHLGNSFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKKK 322

Query: 330 ENLQFTPLLKSPIYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSGTT 389
               FT +L++P +P +Y + L+ I+IG  +          LR ID  G GG+++DSGTT
Sbjct: 323 NEFVFTEMLENPKHPYFYSVSLQGISIGKRN----IPAPAMLRRIDKNGGGGVVVDSGTT 382

Query: 390 YTHLPEPLYSQLISNLELVIS--YPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLPSI 449
           +T LP   Y+ ++   +  +   + RA +VE ++G   CY +             ++P++
Sbjct: 383 FTMLPAKFYNSVVEEFDSRVGRVHERADRVEPSSGMSPCYYLN---------QTVKVPAL 442

Query: 450 TFHFL-NNVSVVLPQGNNFYAMA----APINSTVVKCLLYQSMDGVGDDNDSDDNGPAGI 507
             HF  N  SV LP+ N FY              + CL+  +    G D      G   I
Sbjct: 443 VLHFAGNRSSVTLPRRNYFYEFMDGGDGKEEKRKIGCLMLMN----GGDESELRGGTGAI 494

[Close]

BLAST of PI0005883.1 vs. TAIR 10
Match: AT3G52500.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 178.7 bits (452), Expect = 1.2e-44
Identity = 134/424 (31.60%), Postives = 190/424 (44.81%), Query Frame = 0


Query: 89  GYLMSLTIGTPPQVVQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNISGPKLAAFLPTHS 148
           GY +SL+ GTP Q +    DTGS L W+PC +  + C  C+   + +    +  F+P +S
Sbjct: 89  GYSVSLSFGTPSQTIPFVFDTGSSLVWLPCTS-RYLCSGCD--FSGLDPTLIPRFIPKNS 148

Query: 149 STSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKGTCPRPCPSFAYTYGASGVVTG 208
           S+S    C S  C  ++    P   C   GC   T     C   CP +   YG       
Sbjct: 149 SSSKIIGCQSPKCQFLY---GPNVQC--RGCDPNT---RNCTVGCPPYILQYGLGSTAGV 208

Query: 209 SLTRDVLFMHGNYHNNNDDNNNNKKIPRFCFGCVGATYREPIGIAGFGRGLLSLPFQLGF 268
            +T  + F              +  +P F  GC   + R+P GIAGFGRG +SLP Q+  
Sbjct: 209 LITEKLDF-------------PDLTVPDFVVGCSIISTRQPAGIAGFGRGPVSLPSQMNL 268

Query: 269 SHKGFSHCFLPFKFSNNPNFSSPLILGNLA---ISSKDENLQFTPLLKSPIYPN-----Y 328
             K FSHC +  +F ++ N ++ L L   +     SK   L +TP  K+P   N     Y
Sbjct: 269 --KRFSHCLVSRRF-DDTNVTTDLDLDTGSGHNSGSKTPGLTYTPFRKNPNVSNKAFLEY 328

Query: 329 YYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSGTTYTHLPEPLYSQLISNLE 388
           YY+ L  I +G         + +K     T G+GG ++DSG+T+T +  P++  +     
Sbjct: 329 YYLNLRRIYVGRK----HVKIPYKYLAPGTNGDGGSIVDSGSTFTFMERPVFELVAEEFA 388

Query: 389 LVIS-YPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLPSITFHFLNNVSVVLPQGNNF 448
             +S Y R K +E  TG   C+ +  K        D  +P + F F     + LP  N F
Sbjct: 389 SQMSNYTREKDLEKETGLGPCFNISGKG-------DVTVPELIFEFKGGAKLELPLSNYF 448

Query: 449 YAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQQNLEVVYDLEKERLGFQ 504
                 + +T   CL   S   V   N S   GPA I GSFQQQN  V YDLE +R GF 
Sbjct: 449 ----TFVGNTDTVCLTVVSDKTV---NPSGGTGPAIILGSFQQQNYLVEYDLENDRFGFA 467

[Close]

BLAST of PI0005883.1 vs. TAIR 10
Match: AT3G25700.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 150.2 bits (378), Expect = 4.6e-36
Identity = 142/512 (27.73%), Postives = 203/512 (39.65%), Query Frame = 0


Query: 13  FLSLFLL----LVHISTQT----LATNPKTNFPKDSLVLGLVHSRTSLLT-PKKGYNFIS 72
           FLSLFLL    +  +S       L    K+ FP  +  L L   R   L+  +K   F+ 
Sbjct: 11  FLSLFLLPPSNIAAVSNHNKYLKLPLLRKSPFPSPTQALALDTRRLHFLSLRRKPIPFVK 70

Query: 73  KKRMKAMDQMDGDDNVIEPLREIRDGYLMSLTIGTPPQVVQVYMDTGSDLTWVPCGNLSF 132
              +       G              Y + L IG PPQ + +  DTGSDL WV C     
Sbjct: 71  SPVVSGAASGSGQ-------------YFVDLRIGQPPQSLLLIADTGSDLVWVKCS---- 130

Query: 133 DCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLAT 192
            C++C  +           F P HSST     C    C  +   D        A     T
Sbjct: 131 ACRNCSHHS------PATVFFPRHSSTFSPAHCYDPVCRLVPKPDR-------APICNHT 190

Query: 193 LVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNDDNNNNKKIPRFCFGC-- 252
            +  TC      + Y Y    + +G   R+   +          +    ++    FGC  
Sbjct: 191 RIHSTC-----HYEYGYADGSLTSGLFARETTSL-------KTSSGKEARLKSVAFGCGF 250

Query: 253 -------VGATYREPIGIAGFGRGLLSLPFQLG--FSHKGFSHCFLPFKFSNNPNFSSPL 312
                   G ++    G+ G GRG +S   QLG  F +K FS+C + +  S  P  +S L
Sbjct: 251 RISGQSVSGTSFNGANGVMGLGRGPISFASQLGRRFGNK-FSYCLMDYTLSPPP--TSYL 310

Query: 313 ILGNLAISSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKG 372
           I+GN         L FTPLL +P+ P +YY+ L+S+ +    N  +  +   + EID  G
Sbjct: 311 IIGN--GGDGISKLFFTPLLTNPLSPTFYYVKLKSVFV----NGAKLRIDPSIWEIDDSG 370

Query: 373 NGGMLIDSGTTYTHLPEPLYSQLISNLELVISYPRAKQVELNTGFDLCYKVPCKNNNSSF 432
           NGG ++DSGTT   L EP Y  +I+ +   +  P A    L  GFDLC  V     +   
Sbjct: 371 NGGTVVDSGTTLAFLAEPAYRSVIAAVRRRVKLPIAD--ALTPGFDLCVNV-----SGVT 430

Query: 433 VDDSQLPSITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMD-GVGDDNDSDDN 492
             +  LP + F F      V P  N F           ++CL  QS+D  VG        
Sbjct: 431 KPEKILPRLKFEFSGGAVFVPPPRNYFIE-----TEEQIQCLAIQSVDPKVG-------- 449

Query: 493 GPAGIFGSFQQQNLEVVYDLEKERLGFQPMDC 504
               + G+  QQ     +D ++ RLGF    C
Sbjct: 491 --FSVIGNLMQQGFLFEFDRDRSRLGFSRRGC 449

[Close]

BLAST of PI0005883.1 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 149.8 bits (377), Expect = 6.0e-36
Identity = 157/530 (29.62%), Postives = 223/530 (42.08%), Query Frame = 0


Query: 2   TSISSISIATKFLSLFLLLVHISTQTLATNPKT---NFPKDSLVLGLVH-----SRTSLL 61
           +S SS  +   FL LF  L+ +S+   +   +T   N P+    L L H     + T + 
Sbjct: 3   SSSSSSLLFPFFLILFSCLISVSSSRRSLIDRTLPKNLPRSGFRLSLRHVDSGKNLTKIQ 62

Query: 62  TPKKGYN--FISKKRMKAM------DQMDGDDNVIEPLREIRDGYLMSLTIGTPPQVVQV 121
             ++G N  F    R+ A+       + D  +N+  P       +LM L+IG P      
Sbjct: 63  KIQRGINRGFHRLNRLGAVAVLAVASKPDDTNNIKAPTHGGSGEFLMELSIGNPAVKYSA 122

Query: 122 YMDTGSDLTWVPCGNLSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIH 181
            +DTGSDL W  C      C +C +    I       F P  SS+  +  C S  C  + 
Sbjct: 123 IVDTGSDLIWTQCK----PCTECFDQPTPI-------FDPEKSSSYSKVGCSSGLCNALP 182

Query: 182 SSDNPFDPCTIAGCSLATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNN 241
            S+   D             K  C      + YTYG      G L  +            
Sbjct: 183 RSNCNED-------------KDAC-----EYLYTYGDYSSTRGLLATETFTF-------- 242

Query: 242 DDNNNNKKIPRFCFGC----VGATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFK 301
           +D N+   I    FGC     G  + +  G+ G GRG LSL  QL      FS+C    +
Sbjct: 243 EDENS---ISGIGFGCGVENEGDGFSQGSGLVGLGRGPLSLISQL--KETKFSYCLTSIE 302

Query: 302 FSNNPNFSSPLILGNLA--------ISSKDENLQFTPLLKSPIYPNYYYIGLESITIGNG 361
            S     SS L +G+LA         S   E  +   LL++P  P++YY+ L+ IT+G  
Sbjct: 303 DS---EASSSLFIGSLASGIVNKTGASLDGEVTKTMSLLRNPDQPSFYYLELQGITVGAK 362

Query: 362 DNNFRFGVSFKLREIDTKGNGGMLIDSGTTYTHLPEPLYSQLISNLELVISYPRAKQVEL 421
               R  V     E+   G GGM+IDSGTT T+L E  +  L       +S P       
Sbjct: 363 ----RLSVEKSTFELAEDGTGGMIIDSGTTITYLEETAFKVLKEEFTSRMSLP--VDDSG 422

Query: 422 NTGFDLCYKVPCKNNNSSFVDDSQLPSITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKC 481
           +TG DLC+K+P    N +      +P + FHF     + LP G N+  M A  +ST V C
Sbjct: 423 STGLDLCFKLPDAAKNIA------VPKMIFHF-KGADLELP-GENY--MVAD-SSTGVLC 458

Query: 482 LLYQSMDGVGDDNDSDDNGPAGIFGSFQQQNLEVVYDLEKERLGFQPMDC 504
           L   S +G+             IFG+ QQQN  V++DLEKE + F P +C
Sbjct: 483 LAMGSSNGM------------SIFGNVQQQNFNVLHDLEKETVSFVPTEC 458

The following BLAST results are available for this feature:

BLAST of PI0005883.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q940R4	5.8e-52	32.89	Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g1656...	[more]
Q766C2	4.2e-34	28.76	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q9LNJ3	2.5e-31	28.15	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q766C3	1.3e-30	28.86	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
O04496	9.0e-29	26.17	Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1	[more]

BLAST of PI0005883.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LYP0	1.4e-298	96.55	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G70459...	[more]
A0A5A7TNC9	2.3e-298	96.92	Aspartic proteinase nepenthesin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3CAK9	2.3e-298	96.92	aspartic proteinase nepenthesin-2 OS=Cucumis melo OX=3656 GN=LOC103498305 PE=3 S...	[more]
A0A6J1CMP8	9.4e-239	79.40	probable aspartyl protease At4g16563 OS=Momordica charantia OX=3673 GN=LOC111012...	[more]
A0A6J1EHM1	2.3e-237	80.74	probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC1114342...	[more]

BLAST of PI0005883.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004145478.2	2.8e-298	96.55	probable aspartyl protease At4g16563 [Cucumis sativus] >KGN66888.1 hypothetical ...	[more]
XP_008459091.1	4.8e-298	96.92	PREDICTED: aspartic proteinase nepenthesin-2 [Cucumis melo] >KAA0043307.1 aspart...	[more]
XP_038893627.1	2.1e-269	89.15	probable aspartyl protease At4g16563 [Benincasa hispida]	[more]
XP_023520027.1	1.3e-239	81.76	probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo]	[more]
XP_022142611.1	1.9e-238	79.40	probable aspartyl protease At4g16563 [Momordica charantia]	[more]

BLAST of PI0005883.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G45120.1	1.3e-168	59.14	Eukaryotic aspartyl protease family protein	[more]
AT4G16563.1	4.1e-53	32.89	Eukaryotic aspartyl protease family protein	[more]
AT3G52500.1	1.2e-44	31.60	Eukaryotic aspartyl protease family protein	[more]
AT3G25700.1	4.6e-36	27.73	Eukaryotic aspartyl protease family protein	[more]
AT2G03200.1	6.0e-36	29.62	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 321..499 e-value: 2.3E-25 score: 89.3
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 296..509 e-value: 2.3E-44 score: 153.2
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 84..295 e-value: 5.3E-30 score: 106.8
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 89..505
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 90..296 e-value: 5.9E-28 score: 98.3
None	No IPR available	PANTHER	PTHR47967:SF47	CHLOROPLAST NUCLEOID DNA-BINDING PROTEIN-LIKE	coord: 50..508
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 50..508
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 356..367
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 90..499 score: 33.454998
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 89..503 e-value: 2.44451E-69 score: 221.368

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
PI0005883	PI0005883	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0005883.1-exon	PI0005883.1-exon-chr12:14395963..14397528	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0005883.1-cds	PI0005883.1-cds-chr12:14395963..14397528	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
PI0005883.1	PI0005883.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004190	aspartic-type endopeptidase activity