MS020514.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS020514.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	4-hydroxy-3-methylbut-2-enyl diphosphate reductase
Location	scaffold375: 797107 .. 800042 (-)
Sequence length	1392
RNA-Seq Expression	MS020514.1
Synteny	MS020514.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGATTTCTCTGCAGATCCCCCGCGTTCCCCATCGCGCAGCCTTCATTTCACCCAGTCCCCTCTCCGCCGCCGGAGCTCTGCCTCTTCGCCTTCGGAAACCCTTTTCCCTCCGGTGCACCGCCGATTCTCCGTCATCTCCGGGGCCGCTCGATCCCGATTTCGACGCGAAGTTGTTCTGGAAGAACTGGGCCAGAGGTGAAAACTACAACCGGAAAGGGTTTGGGCACAAGGAGGAGACTATGAAGCTCATGAACAGCGAGTTCACGAGTGAGTTTTCTTGTATTCGATTGTACCAATATCGTGATGGTTTTAAACGATTTCTGTGTTGAATGGCTCAGGTGAAGTTATAAATACTCTGAATGAAAATGGGAACGAGTTCACTTGGGGAAATGTTACTGTGAAATTGGCTCGTGCTTATGGCTTCTGCTATGGAGTTGAGCGAGCTGTGCAGATTGCTTTCGAAGCGAGGAAACAGTTTCCGAGTAGAACCATTTGGATTACCAATGAAATCATTCACAACCCCACTGTTAACAAGGTCCTCTGAACTTTTCTTGCCTTTACCATTACTATTTCATTAGCTGGATTCCACAGAAATTATAGTGTATACCACTTCCTGTTCATATTTAGCTACAATTACTATCATATTTAGCTCGAGACTCACATAACTTTAAAATTCAAAGACATGGTTATGAAAGAATGATCTCAGATAACATACTCTTTCTTTTTGTGTTGTTTTGTTCTGTTTTGCATTTATGGTTGTGTTGTAGCCGAAAACATGATGTGTTGTACCTGAAATGAGATATCATTTGGTAACTCTTTGGACGATTCTATGAGTACAGCGTTTGGGGGAGATGGGAGTGCATTACATTCCTCTAGAGGAAAGAAAGAAACAGTTCGATGTCGTTAATCCTGGCGATGTTGTGATTTTGCCTACCTTTGGAGCTGCAGTCGACGAAATGCTTGTTTTGAATGATAAAAATGTCCAGATTGTTGACACAACTTGCCCATGGGTTTCCAAGGTAAGCTTGTCATTTACTTGTCCTTCACAATTCTCTCCTCAGAAGGAAAGACATCCTTGTATTGTATGCTGTCTTCTGTCGTTATTTTTTCCTTTTGGTAAATGTTCCACTTAGTAACATTATGCAGCTGTTGATGCTTAGATTATACTGCACTTCTTTCGTGTAGGTTTGGAACGTCGTTGACAAACACAAAAAAAGGGAATACACGTCAATAATTCATGGTAAATATCGGCATGAAGAAACCATAGCAACTGCTTCTTTCGCTGGAAAGTACATCATTGTGAAAAACATGGCAGAGGTACATCCTTGGCAGTCTTGTCCCTTTTCTAACATTTCAAACTCTTGGCACATAGCATAAGTAAGCATTTTAAGAGTAGAAGTTTGAACATGGGTCAGGCAACATATGTAAGCGACTTTATTCTCGGGGTTGAACGTAATGGATCGATCTTAACTAAAGAAGAATTTTTGAAGGTACAACCTTTTATTTTAGCTATTTACTTAACTGATCATGCTTAATATTTTACAACATGCACGATACGAGTCAAGTTTCTGGCTTGTTTGTAGAAATTTGAGTCTGCTGTTTCGAGAGGATTTGATCCAGATTATGATCTTATAAAAGTTGGCATTGCTAATCAGACCACAATGCTCAAAGGAGAAACAGAAGAGATTGGTTAGTATTTAGAGCTATTGAATGCTATATCAAATCATTGTTTATCGACAAGCTTGTATTCTCGTGGATTATTGATTTTGTGATTCTGTTAACAGGAAAATTGATAGAGAGAACTATGATGCGTAAGTATGGAGTTGAAAATGTCAATGAGCACTTCGTTAGTTTCAATACGATCTGCGACGCTACTCAAGTAAGTACCGTTCTTTTTGTAACGTATAGCTTATCGGAAGGGAATCTTTACAAAAACTCCCACCATCTTAATTGGCTCATTTTCATATTAAGAAACTTATAGATGAAGGGAAGGTTTCCATTATGGATATGTTAAGAGTGCAGCTATGAAGTCCTATGTAATAATGCATAAGATGATAGGTTTATATGGACAATATCTCCATTAATGTCAAGTCTTTTGGGTTAGTACAAAAGTCGATTAATCTAAACTTTAGACCTAAAATAGACAATATCATATTGATGAGAGTCGTGCTCGTAGTATCTACTCATGTAGTGGTTCTATGGTCCCATCCCGCCATGGTGTGGTCAACCTGAAACCCCAACGGGCACCACGCCACACGAAATTCGTGTGGATCAAGACTAGAATTAGGACAATAATCATTGATTAGTTCCTCTTTGCGTACGTTATGAATAGTGTACTAATACTCCAGAAAGCCCATGATAAAGACTAGAGACATTATCTTCCATCCTCAGGAGCGCCAAGATGCAATGTACCAGTTGGTGGAGGAAGATATGGATCTCATGTTGGTTGTTGGTGGATGGAACTCAAGCAACACCTCTCATCTTCAAGAGATTGCCGAGGCTCGAGGAATCCCGTCATATTGGATCGACAGCGAGCAGCGAATAGGTCCAGGAAACTCAATATCATACAAGCTCATTGTAAGTCTGAATTCAGAGCGTTGATTCAAACATCCTACCTGCCATCTTTTTCCTCTTTCCAACTCATGTTTACTCGTTTAATCCTACGACAGCACGGGGAGCTGGCCGAGAAAGAAAACTGGCTACCTGATGGTCATGTCACCATTGGAGTGACCTCCGGTGCCTCCACACCAGATAAGGTTAGTGAAAAGTTAACTGTAAAAACCTTGGAATGTTCAATGTGACTGAAAACTGTGAATCTGCCATTTTCATTTGACATTTAACATTTTTGTCAAATGGGTTGAATTTTTAGGTTGTTGAAAATGTTCTTAAGAAGGTTTTTGACGTCAAAGGGGAAGGAACTGTGCAACTTGCA

mRNA sequence

ATGGCGATTTCTCTGCAGATCCCCCGCGTTCCCCATCGCGCAGCCTTCATTTCACCCAGTCCCCTCTCCGCCGCCGGAGCTCTGCCTCTTCGCCTTCGGAAACCCTTTTCCCTCCGGTGCACCGCCGATTCTCCGTCATCTCCGGGGCCGCTCGATCCCGATTTCGACGCGAAGTTGTTCTGGAAGAACTGGGCCAGAGGTGAAAACTACAACCGGAAAGGGTTTGGGCACAAGGAGGAGACTATGAAGCTCATGAACAGCGAGTTCACGAGTGAAGTTATAAATACTCTGAATGAAAATGGGAACGAGTTCACTTGGGGAAATGTTACTGTGAAATTGGCTCGTGCTTATGGCTTCTGCTATGGAGTTGAGCGAGCTGTGCAGATTGCTTTCGAAGCGAGGAAACAGTTTCCGAGTAGAACCATTTGGATTACCAATGAAATCATTCACAACCCCACTGTTAACAAGCGTTTGGGGGAGATGGGAGTGCATTACATTCCTCTAGAGGAAAGAAAGAAACAGTTCGATGTCGTTAATCCTGGCGATGTTGTGATTTTGCCTACCTTTGGAGCTGCAGTCGACGAAATGCTTGTTTTGAATGATAAAAATGTCCAGATTGTTGACACAACTTGCCCATGGGTTTCCAAGGTTTGGAACGTCGTTGACAAACACAAAAAAAGGGAATACACGTCAATAATTCATGGTAAATATCGGCATGAAGAAACCATAGCAACTGCTTCTTTCGCTGGAAAGTACATCATTGTGAAAAACATGGCAGAGGCAACATATGTAAGCGACTTTATTCTCGGGGTTGAACGTAATGGATCGATCTTAACTAAAGAAGAATTTTTGAAGAAATTTGAGTCTGCTGTTTCGAGAGGATTTGATCCAGATTATGATCTTATAAAAGTTGGCATTGCTAATCAGACCACAATGCTCAAAGGAGAAACAGAAGAGATTGGAAAATTGATAGAGAGAACTATGATGCGTAAGTATGGAGTTGAAAATGTCAATGAGCACTTCGTTAGTTTCAATACGATCTGCGACGCTACTCAAGAGCGCCAAGATGCAATGTACCAGTTGGTGGAGGAAGATATGGATCTCATGTTGGTTGTTGGTGGATGGAACTCAAGCAACACCTCTCATCTTCAAGAGATTGCCGAGGCTCGAGGAATCCCGTCATATTGGATCGACAGCGAGCAGCGAATAGGTCCAGGAAACTCAATATCATACAAGCTCATTCACGGGGAGCTGGCCGAGAAAGAAAACTGGCTACCTGATGGTCATGTCACCATTGGAGTGACCTCCGGTGCCTCCACACCAGATAAGGTTGTTGAAAATGTTCTTAAGAAGGTTTTTGACGTCAAAGGGGAAGGAACTGTGCAACTTGCA

Coding sequence (CDS)

ATGGCGATTTCTCTGCAGATCCCCCGCGTTCCCCATCGCGCAGCCTTCATTTCACCCAGTCCCCTCTCCGCCGCCGGAGCTCTGCCTCTTCGCCTTCGGAAACCCTTTTCCCTCCGGTGCACCGCCGATTCTCCGTCATCTCCGGGGCCGCTCGATCCCGATTTCGACGCGAAGTTGTTCTGGAAGAACTGGGCCAGAGGTGAAAACTACAACCGGAAAGGGTTTGGGCACAAGGAGGAGACTATGAAGCTCATGAACAGCGAGTTCACGAGTGAAGTTATAAATACTCTGAATGAAAATGGGAACGAGTTCACTTGGGGAAATGTTACTGTGAAATTGGCTCGTGCTTATGGCTTCTGCTATGGAGTTGAGCGAGCTGTGCAGATTGCTTTCGAAGCGAGGAAACAGTTTCCGAGTAGAACCATTTGGATTACCAATGAAATCATTCACAACCCCACTGTTAACAAGCGTTTGGGGGAGATGGGAGTGCATTACATTCCTCTAGAGGAAAGAAAGAAACAGTTCGATGTCGTTAATCCTGGCGATGTTGTGATTTTGCCTACCTTTGGAGCTGCAGTCGACGAAATGCTTGTTTTGAATGATAAAAATGTCCAGATTGTTGACACAACTTGCCCATGGGTTTCCAAGGTTTGGAACGTCGTTGACAAACACAAAAAAAGGGAATACACGTCAATAATTCATGGTAAATATCGGCATGAAGAAACCATAGCAACTGCTTCTTTCGCTGGAAAGTACATCATTGTGAAAAACATGGCAGAGGCAACATATGTAAGCGACTTTATTCTCGGGGTTGAACGTAATGGATCGATCTTAACTAAAGAAGAATTTTTGAAGAAATTTGAGTCTGCTGTTTCGAGAGGATTTGATCCAGATTATGATCTTATAAAAGTTGGCATTGCTAATCAGACCACAATGCTCAAAGGAGAAACAGAAGAGATTGGAAAATTGATAGAGAGAACTATGATGCGTAAGTATGGAGTTGAAAATGTCAATGAGCACTTCGTTAGTTTCAATACGATCTGCGACGCTACTCAAGAGCGCCAAGATGCAATGTACCAGTTGGTGGAGGAAGATATGGATCTCATGTTGGTTGTTGGTGGATGGAACTCAAGCAACACCTCTCATCTTCAAGAGATTGCCGAGGCTCGAGGAATCCCGTCATATTGGATCGACAGCGAGCAGCGAATAGGTCCAGGAAACTCAATATCATACAAGCTCATTCACGGGGAGCTGGCCGAGAAAGAAAACTGGCTACCTGATGGTCATGTCACCATTGGAGTGACCTCCGGTGCCTCCACACCAGATAAGGTTGTTGAAAATGTTCTTAAGAAGGTTTTTGACGTCAAAGGGGAAGGAACTGTGCAACTTGCA

Protein sequence

MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLFWKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFCYGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVNPGDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRHEETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDYDLIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQERQDAMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIHGELAEKENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQLA

Homology

[Close]

BLAST of MS020514.1 vs. NCBI nr
Match: XP_022148542.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 932.2 bits (2408), Expect = 1.8e-267
Identity = 460/465 (98.92%), Postives = 462/465 (99.35%), Query Frame = 0


Query: 1   MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60
           MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF
Sbjct: 1   MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60

Query: 61  WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120
           WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC
Sbjct: 61  WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120

Query: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVNP 180
           YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVNP
Sbjct: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVNP 180

Query: 181 GDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRHE 240
           GDVVILP FGAAVDEMLVL DKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRHE
Sbjct: 181 GDVVILPAFGAAVDEMLVLKDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRHE 240

Query: 241 ETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDYD 300
           ETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDYD
Sbjct: 241 ETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDYD 300

Query: 301 LIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQ-ERQDAMY 360
           LIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQ +RQDAMY
Sbjct: 301 LIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQVKRQDAMY 360

Query: 361 QLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIHGELA 420
           QLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLI+GELA
Sbjct: 361 QLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIYGELA 420

Query: 421 EKENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQLA 465
           EKENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQLA
Sbjct: 421 EKENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQLA 465

[Close]

BLAST of MS020514.1 vs. NCBI nr
Match: XP_038902065.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X2 [Benincasa hispida])

HSP 1 Score: 797.7 bits (2059), Expect = 5.2e-227
Identity = 389/463 (84.02%), Postives = 421/463 (90.93%), Query Frame = 0


Query: 1   MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60
           MAISLQIPR P R  F+S    S  G   LR RKP  LRCTADS SSP P+DPDFDAKLF
Sbjct: 1   MAISLQIPRFPLRIDFVSRKTFS--GVRALRCRKPLFLRCTADSSSSPEPIDPDFDAKLF 60

Query: 61  WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120
           WKN++RGENYNRKGFGHKEET+KLM+SEFTSE++NTLNENG +FTWGNVTVKLA AYGFC
Sbjct: 61  WKNFSRGENYNRKGFGHKEETVKLMSSEFTSEIVNTLNENGKQFTWGNVTVKLAGAYGFC 120

Query: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVNP 180
           +GVERAVQIA+EARKQFPS TIWITNEIIHNPTVNKRL EMGV+ IPLEE KKQF+VVNP
Sbjct: 121 WGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKQFNVVNP 180

Query: 181 GDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRHE 240
           GDVVILP FGAAVDEM+VLNDK V+IVDTTCPWVSKVWN VDKHKK EYTSIIHGKYRHE
Sbjct: 181 GDVVILPAFGAAVDEMIVLNDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHE 240

Query: 241 ETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDYD 300
           ETIATASFAGKYIIVK+MAEA  V D+ILGVE NG I TK EFLKKF+SAVS+GFDPD D
Sbjct: 241 ETIATASFAGKYIIVKDMAEAMDVCDYILGVEHNGLISTKAEFLKKFKSAVSKGFDPDID 300

Query: 301 LIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQERQDAMYQ 360
           L+K+GI+NQTTMLK ETEEIGKL+E+TMMRKYGVEN+NEHF+SFNTIC+ATQ RQDAMY+
Sbjct: 301 LMKIGISNQTTMLKRETEEIGKLMEKTMMRKYGVENINEHFISFNTICNATQVRQDAMYE 360

Query: 361 LVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIHGELAE 420
           LVEED+DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKL HGELA 
Sbjct: 361 LVEEDVDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLTHGELAN 420

Query: 421 KENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQL 464
           KENWLPDGH+TIGVTSGASTPDKVV NVLKKVFD+KG+GT QL
Sbjct: 421 KENWLPDGHITIGVTSGASTPDKVVGNVLKKVFDIKGDGTAQL 461

[Close]

BLAST of MS020514.1 vs. NCBI nr
Match: XP_004145400.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Cucumis sativus] >KAE8651867.1 hypothetical protein Csa_006064 [Cucumis sativus])

HSP 1 Score: 795.4 bits (2053), Expect = 2.6e-226
Identity = 386/463 (83.37%), Postives = 419/463 (90.50%), Query Frame = 0


Query: 1   MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60
           MAISLQ P  PHR  F SP   S  GA  LR RKP  LRCTADS SSP P+DPDFD KLF
Sbjct: 1   MAISLQSPCFPHRIQFFSPKTFSGVGA--LRCRKPLLLRCTADSSSSPKPVDPDFDPKLF 60

Query: 61  WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120
            KN++RGENYNRKGFGHKEET+KLMNSEFTSE++NTLNENG +FTWG VTVKLA AYGFC
Sbjct: 61  LKNFSRGENYNRKGFGHKEETIKLMNSEFTSEIVNTLNENGYQFTWGKVTVKLAGAYGFC 120

Query: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVNP 180
           +GVERAVQ A+EARKQ+PS TIWITNEIIHNPTVNKRL EMGVH IPLEE  K+FDVVNP
Sbjct: 121 WGVERAVQFAYEARKQYPSETIWITNEIIHNPTVNKRLEEMGVHNIPLEEGNKRFDVVNP 180

Query: 181 GDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRHE 240
           GDVVILP FGAAVDE+LVLNDK V+IVDTTCPWVSKVWN V KHKK EYTSIIHGKYRHE
Sbjct: 181 GDVVILPAFGAAVDEILVLNDKKVKIVDTTCPWVSKVWNAVGKHKKGEYTSIIHGKYRHE 240

Query: 241 ETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDYD 300
           ETIATASFAG+YIIVK+MAEA  VSD+ILGVE NGSI TKEEFLKKF+SAVS+GFDPD D
Sbjct: 241 ETIATASFAGRYIIVKDMAEAACVSDYILGVEHNGSISTKEEFLKKFKSAVSKGFDPDID 300

Query: 301 LIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQERQDAMYQ 360
           L+K+GIANQTTMLKGETEEIGKL+E+TMMRKYGVEN+NEHF+SFNTIC+ATQ RQDAMY+
Sbjct: 301 LMKIGIANQTTMLKGETEEIGKLMEKTMMRKYGVENINEHFISFNTICNATQVRQDAMYE 360

Query: 361 LVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIHGELAE 420
           LV+ED+DLMLVVGGWNSSNTSHLQEIAEARG+PSYWIDSEQRIGPGNSISYKL HGELA 
Sbjct: 361 LVKEDVDLMLVVGGWNSSNTSHLQEIAEARGVPSYWIDSEQRIGPGNSISYKLAHGELAN 420

Query: 421 KENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQL 464
           KENWLPDGH+TIGVTSGASTPDKVVE+VLKKVFD+KG+G  QL
Sbjct: 421 KENWLPDGHITIGVTSGASTPDKVVEDVLKKVFDIKGDGNAQL 461

[Close]

BLAST of MS020514.1 vs. NCBI nr
Match: XP_023512039.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 794.7 bits (2051), Expect = 4.4e-226
Identity = 390/463 (84.23%), Postives = 422/463 (91.14%), Query Frame = 0


Query: 1   MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60
           MAISLQIPR+P R   +S    S AGA  LR RKP  +RCTADS S    ++PDFDAKLF
Sbjct: 1   MAISLQIPRLPRRVDSVSSKSFSGAGA--LRFRKPLLVRCTADSSS----VNPDFDAKLF 60

Query: 61  WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120
           WKN++RGENYNRKGFG+KEE +KLM+SEFTSEV+NTLNENGN FTWGNVTVKLA AYGFC
Sbjct: 61  WKNFSRGENYNRKGFGYKEEALKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFC 120

Query: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVNP 180
           +GVERAVQIA+EARKQFPS TIWITNEIIHNPTVNKRL EMGV+ IPLEE KK+FDVVNP
Sbjct: 121 WGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNP 180

Query: 181 GDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRHE 240
           GDVVILP FGAAVDEMLVLNDK V+IVDTTCPWVSKVWN VDKHKK EYTSIIHGKYRHE
Sbjct: 181 GDVVILPAFGAAVDEMLVLNDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHE 240

Query: 241 ETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDYD 300
           ET+ATASFAGKYIIVK++ EATYV D+ILGVE NG I TKE FLKKF+SAVS+GFDPD D
Sbjct: 241 ETVATASFAGKYIIVKDIVEATYVCDYILGVESNGLISTKEAFLKKFKSAVSKGFDPDSD 300

Query: 301 LIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQERQDAMYQ 360
           L+KVGIANQTTMLKGETEEIGKLIE+TMMRKYG+EN+N+HF+SFNTIC+ATQ RQDAMYQ
Sbjct: 301 LMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQ 360

Query: 361 LVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIHGELAE 420
           LVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKL HGELA+
Sbjct: 361 LVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAK 420

Query: 421 KENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQL 464
           KENWLPDGHVTIGVTSGASTPDKVV NVLKKVF++KGEGT QL
Sbjct: 421 KENWLPDGHVTIGVTSGASTPDKVVGNVLKKVFEIKGEGTAQL 457

[Close]

BLAST of MS020514.1 vs. NCBI nr
Match: XP_022944389.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 790.0 bits (2039), Expect = 1.1e-224
Identity = 389/463 (84.02%), Postives = 422/463 (91.14%), Query Frame = 0


Query: 1   MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60
           MAISLQIPR+  R   +S    SA GA  LR RKP  +RCTADS S    ++PDFDAKLF
Sbjct: 1   MAISLQIPRLHLRVDSVSSKRFSAGGA--LRCRKPLLVRCTADSSS----VNPDFDAKLF 60

Query: 61  WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120
           WKN++RGENYNRKGFGHKEET+KLM+SEFTSEV+NTLNENGN FTWGNVTVKLA AYGFC
Sbjct: 61  WKNFSRGENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFC 120

Query: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVNP 180
           +GVERAVQIA+EARKQFPS TIWITNEIIHNPTVNKRL EMGV+ IPLEE KK+FDVVNP
Sbjct: 121 WGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNP 180

Query: 181 GDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRHE 240
           GDVVILP FGAAVDEMLVL+DK V+IVDTTCPWVSKVWN VDKHKK EYTSIIHGKYRHE
Sbjct: 181 GDVVILPAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHE 240

Query: 241 ETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDYD 300
           ET+ATASFAGKYIIVK+M EATYV D+ILGVE NG I TKE FLKKF+SA+S+GFDPD D
Sbjct: 241 ETVATASFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDPDSD 300

Query: 301 LIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQERQDAMYQ 360
           L+KVGIANQTTMLKGETEEIGKLIE+TMMRKYG+EN+N+HF+SFNTIC+ATQ RQDAMYQ
Sbjct: 301 LMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQ 360

Query: 361 LVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIHGELAE 420
           LVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKL HGELA+
Sbjct: 361 LVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAK 420

Query: 421 KENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQL 464
           KENWLPDGHVTIGVTSGASTPDKVV NVLKKVF++KGEGT Q+
Sbjct: 421 KENWLPDGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQV 457

[Close]

BLAST of MS020514.1 vs. ExPASy Swiss-Prot
Match: Q94B35 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISPH PE=1 SV=1)

HSP 1 Score: 685.3 bits (1767), Expect = 4.9e-196
Identity = 336/468 (71.79%), Postives = 394/468 (84.19%), Query Frame = 0


Query: 1   MAISLQIPRVPHRA-AFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGP---LDPDFD 60
           MA++LQ  R+  R   F+  + LS +G+  LR RK  S+RC++   ++P P   +D DFD
Sbjct: 1   MAVALQFSRLCVRPDTFVRENHLSGSGS--LRRRKALSVRCSSGDENAPSPSVVMDSDFD 60

Query: 61  AKLFWKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARA 120
           AK+F KN  R +NYNRKGFGHKEET+KLMN E+TS+++ TL  NG  ++WG+VTVKLA+A
Sbjct: 61  AKVFRKNLTRSDNYNRKGFGHKEETLKLMNREYTSDILETLKTNGYTYSWGDVTVKLAKA 120

Query: 121 YGFCYGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFD 180
           YGFC+GVERAVQIA+EARKQFP   +WITNEIIHNPTVNKRL +M V  IP+E+ KKQFD
Sbjct: 121 YGFCWGVERAVQIAYEARKQFPEERLWITNEIIHNPTVNKRLEDMDVKIIPVEDSKKQFD 180

Query: 181 VVNPGDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGK 240
           VV   DVVILP FGA VDEM VLNDK VQIVDTTCPWV+KVWN V+KHKK EYTS+IHGK
Sbjct: 181 VVEKDDVVILPAFGAGVDEMYVLNDKKVQIVDTTCPWVTKVWNTVEKHKKGEYTSVIHGK 240

Query: 241 YRHEETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFD 300
           Y HEETIATASFAGKYIIVKNM EA YV D+ILG + +GS  TKEEF++KF+ A+S+GFD
Sbjct: 241 YNHEETIATASFAGKYIIVKNMKEANYVCDYILGGQYDGSSSTKEEFMEKFKYAISKGFD 300

Query: 301 PDYDLIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQERQD 360
           PD DL+KVGIANQTTMLKGETEEIG+L+E TMMRKYGVENV+ HF+SFNTICDATQERQD
Sbjct: 301 PDNDLVKVGIANQTTMLKGETEEIGRLLETTMMRKYGVENVSGHFISFNTICDATQERQD 360

Query: 361 AMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIHG 420
           A+Y+LVEE +DLMLVVGGWNSSNTSHLQEI+EARGIPSYWIDSE+RIGPGN I+YKL +G
Sbjct: 361 AIYELVEEKIDLMLVVGGWNSSNTSHLQEISEARGIPSYWIDSEKRIGPGNKIAYKLHYG 420

Query: 421 ELAEKENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQLA 465
           EL EKEN+LP G +TIGVTSGASTPDKVVE+ L KVFD+K E  +QLA
Sbjct: 421 ELVEKENFLPKGPITIGVTSGASTPDKVVEDALVKVFDIKREELLQLA 466

[Close]

BLAST of MS020514.1 vs. ExPASy Swiss-Prot
Match: Q6AVG6 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISPH PE=2 SV=1)

HSP 1 Score: 667.2 bits (1720), Expect = 1.4e-190
Identity = 327/455 (71.87%), Postives = 379/455 (83.30%), Query Frame = 0


Query: 13  RAAFISPSPLSAAGALPLRLRKPFSLRC---TADSPSSPGPLDPDFDAKLFWKNWARGEN 72
           R+A +SP    AA       R P S+RC    A S S+   LD DFD K F  N  R +N
Sbjct: 9   RSALLSP----AASPSRRARRAPSSVRCDSSAASSLSASASLDADFDKKQFRHNLTRSDN 68

Query: 73  YNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFCYGVERAVQI 132
           YNRKGFGHK+ET++LM+ E+TS+VI TL ENGN+ TWG VTVKLA AYGFC+GVERAVQI
Sbjct: 69  YNRKGFGHKKETLELMSQEYTSDVIKTLKENGNQHTWGPVTVKLAEAYGFCWGVERAVQI 128

Query: 133 AFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVNPGDVVILPTF 192
           A+EARKQFP   IW+TNEIIHNPTVNKRL +MGV  IP++   K FDVV  GDVV+LP F
Sbjct: 129 AYEARKQFPDDRIWLTNEIIHNPTVNKRLEDMGVQNIPVDAGIKDFDVVEQGDVVVLPAF 188

Query: 193 GAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRHEETIATASFA 252
           GAAV+EM  LN+K VQIVDTTCPWVSKVWN+V+KHKK +YTSIIHGKY HEET+ATASFA
Sbjct: 189 GAAVEEMYTLNEKKVQIVDTTCPWVSKVWNMVEKHKKGDYTSIIHGKYSHEETVATASFA 248

Query: 253 GKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDYDLIKVGIANQ 312
           G YIIVKN+AEA+YV D+ILG + +GS  TKEEFL+KF++AVS GFDPD DL+KVGIANQ
Sbjct: 249 GTYIIVKNIAEASYVCDYILGGQLDGSSSTKEEFLEKFKNAVSPGFDPDVDLVKVGIANQ 308

Query: 313 TTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQERQDAMYQLVEEDMDLM 372
           TTMLKGETEEIGKL+E+TMMR++GVENVN+HF++FNTICDATQERQDAMYQLV+E +DL+
Sbjct: 309 TTMLKGETEEIGKLVEKTMMRRFGVENVNDHFIAFNTICDATQERQDAMYQLVKEKVDLI 368

Query: 373 LVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIHGELAEKENWLPDGH 432
           LVVGGWNSSNTSHLQEI E  GIPSYWIDSEQRIGPGN ISYKL HGEL EKENWLP+G 
Sbjct: 369 LVVGGWNSSNTSHLQEIGELSGIPSYWIDSEQRIGPGNKISYKLNHGELVEKENWLPEGP 428

Query: 433 VTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQLA 465
           +TIGVTSGASTPDKVVE+ L+KVF++K +  +Q A
Sbjct: 429 ITIGVTSGASTPDKVVEDALQKVFEIKRQEVLQAA 459

[Close]

BLAST of MS020514.1 vs. ExPASy Swiss-Prot
Match: B1WTZ2 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Crocosphaera subtropica (strain ATCC 51142 / BH68) OX=43989 GN=ispH PE=3 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 4.7e-146
Identity = 249/406 (61.33%), Postives = 317/406 (78.08%), Query Frame = 0


Query: 56  DAKLFWKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLAR 115
           D K F ++  +  NY+RKGFGH+ E M  MN+E+ S +I  + EN  ++  G+VT+KLA 
Sbjct: 2   DTKAFKRSLQQSNNYHRKGFGHETEVMGTMNTEYQSSLIQKIRENNYQWQEGDVTIKLAE 61

Query: 116 AYGFCYGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQF 175
           A+GFC+GVERAV +A+E R+ FP   IWITNEIIHNP+VN+RL EM V +I +    K F
Sbjct: 62  AFGFCWGVERAVAMAYETRQHFPQEKIWITNEIIHNPSVNQRLLEMNVGFIEVINGSKDF 121

Query: 176 DVVNPGDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHG 235
            VV  GDVVILP FGA+V EM +LNDK   IVDTTCPWVSKVWN V+KHKK++YTSIIHG
Sbjct: 122 SVVEAGDVVILPAFGASVTEMQLLNDKGCTIVDTTCPWVSKVWNSVEKHKKKDYTSIIHG 181

Query: 236 KYRHEETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGF 295
           KY+HEET+AT+SFAG Y++V N+ EA YV D+IL    NG    K+EFL+KF++A S GF
Sbjct: 182 KYKHEETVATSSFAGTYLVVLNLKEAQYVCDYIL----NGG--NKDEFLEKFKNAHSEGF 241

Query: 296 DPDYDLIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQERQ 355
           DPD DLI++GIANQTTMLK ETE+IGKL E TM++KYG   +NEHF+SFNTICDATQERQ
Sbjct: 242 DPDKDLIRLGIANQTTMLKSETEQIGKLFETTMLKKYGPTELNEHFMSFNTICDATQERQ 301

Query: 356 DAMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIH 415
           DAM  LVEE++D+M+V+GG+NSSNT+HLQEI+  +GI SY IDS++RI PGN I +K + 
Sbjct: 302 DAMLNLVEEEVDVMVVIGGFNSSNTTHLQEISIEKGIDSYHIDSDKRILPGNKIEHKPLE 361

Query: 416 GELAEKENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTV 462
            ++  KENWLPDG + +GVTSGASTPDKVVE V++++F+ K  G +
Sbjct: 362 KDIETKENWLPDGKIIVGVTSGASTPDKVVEAVIERIFEEKNAGVL 401

[Close]

BLAST of MS020514.1 vs. ExPASy Swiss-Prot
Match: B8HWD3 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cyanothece sp. (strain PCC 7425 / ATCC 29141) OX=395961 GN=ispH PE=3 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 6.3e-143
Identity = 246/402 (61.19%), Postives = 312/402 (77.61%), Query Frame = 0


Query: 56  DAKLFWKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLAR 115
           D K F ++      Y+RKGFGH+ E  +++  E+ S +I  + ENGN F  G VT++LA 
Sbjct: 2   DTKAFKRSLHSSNQYHRKGFGHEAEVTEMLRFEYQSSLIQQIRENGNRFQRGEVTIQLAE 61

Query: 116 AYGFCYGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEER-KKQ 175
           A+GFC+GVERAV +A+E R+ FP+  IWITNEIIHNP+VN+ L EM V +IP++E+ +K 
Sbjct: 62  AFGFCWGVERAVALAYETRQHFPTERIWITNEIIHNPSVNQNLREMQVEFIPVDEQGQKD 121

Query: 176 FDVVNPGDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIH 235
           F VV   DVVILP FGA+V EM +LNDK  +I+DTTCPWVSKVWN V+KHKK  YTSIIH
Sbjct: 122 FSVVAGEDVVILPAFGASVQEMQLLNDKGCKIMDTTCPWVSKVWNSVEKHKKGSYTSIIH 181

Query: 236 GKYRHEETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRG 295
           GKY+HEETIAT+S+A  Y+IV ++ EA YV ++IL    NG    +EEFL KF  A S  
Sbjct: 182 GKYQHEETIATSSYAATYLIVLDLKEAEYVCNYIL----NGG--DREEFLHKFRHAYSPD 241

Query: 296 FDPDYDLIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQER 355
           FDPD DL++VGIANQTTMLKGETE+IGKL ERT+MRKYG + +N+HF+SFNTICDATQER
Sbjct: 242 FDPDRDLVRVGIANQTTMLKGETEQIGKLFERTLMRKYGPDQINQHFLSFNTICDATQER 301

Query: 356 QDAMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLI 415
           QDAM++LVE  +DLM+V+GG+NSSNT+HLQEI+  R IPSY IDS  RIGPGN + +K +
Sbjct: 302 QDAMFKLVEYPLDLMVVIGGFNSSNTTHLQEISIEREIPSYHIDSADRIGPGNRVEHKPL 361

Query: 416 HGELAEKENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVK 457
           H  L  KENWLP+G + +G+TSGASTPDKVV +VL+K+F +K
Sbjct: 362 HRPLEIKENWLPEGPIVVGITSGASTPDKVVSDVLEKIFALK 397

[Close]

BLAST of MS020514.1 vs. ExPASy Swiss-Prot
Match: B2IZV5 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Nostoc punctiforme (strain ATCC 29133 / PCC 73102) OX=63737 GN=ispH PE=3 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 2.4e-142
Identity = 243/401 (60.60%), Postives = 312/401 (77.81%), Query Frame = 0


Query: 56  DAKLFWKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLAR 115
           D K F ++    ENYNRKGFGH+ E    + SE+ S +I  + +       G+VT++LA+
Sbjct: 2   DTKAFKRSLQHSENYNRKGFGHQAEVATQLQSEYQSNLIQEIRDRNYILQRGDVTIRLAQ 61

Query: 116 AYGFCYGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQF 175
           A+GFC+GVERAV +A+E R+ FP+  IWITNEIIHNP+VN+R+ EM V +IP+E + K F
Sbjct: 62  AFGFCWGVERAVAMAYETRQHFPTEHIWITNEIIHNPSVNQRMQEMEVEFIPIEGKNKDF 121

Query: 176 DVVNPGDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHG 235
            VV  GDVVILP FGA+V EM +L+DK  +IVDTTCPWVSKVWN V+KHKK +YTSIIHG
Sbjct: 122 SVVGTGDVVILPAFGASVQEMQILHDKGCKIVDTTCPWVSKVWNTVEKHKKIDYTSIIHG 181

Query: 236 KYRHEETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGF 295
           KY+HEET+AT+SFAGKY+IV N+ EA YV+D+I+    NG    +EEFL KF  A S GF
Sbjct: 182 KYKHEETVATSSFAGKYLIVLNLQEAQYVADYII----NGG--NREEFLTKFAKACSAGF 241

Query: 296 DPDYDLIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQERQ 355
           DPD DL +VGIANQTTMLK ETE+IGKL ERTM++KY    +N+HF SFNTICDATQERQ
Sbjct: 242 DPDRDLERVGIANQTTMLKDETEQIGKLFERTMLQKYSPIELNQHFQSFNTICDATQERQ 301

Query: 356 DAMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIH 415
           DAM +LVE +++LM+V+GG+NSSNT+ LQ+IA  R IPSY ID+ +RI  G+SI ++ ++
Sbjct: 302 DAMLELVEHNLNLMVVIGGFNSSNTTQLQQIAFERRIPSYHIDTVERIKSGDSIEHRQLN 361

Query: 416 GELAEKENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVK 457
           G+L   ENWLPDG + +G+TSGASTPDKVVE+V++K+F VK
Sbjct: 362 GQLITTENWLPDGEIVVGITSGASTPDKVVEDVIEKIFAVK 396

[Close]

BLAST of MS020514.1 vs. ExPASy TrEMBL
Match: A0A6J1D5C2 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111017170 PE=3 SV=1)

HSP 1 Score: 932.2 bits (2408), Expect = 8.6e-268
Identity = 460/465 (98.92%), Postives = 462/465 (99.35%), Query Frame = 0


Query: 1   MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60
           MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF
Sbjct: 1   MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60

Query: 61  WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120
           WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC
Sbjct: 61  WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120

Query: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVNP 180
           YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVNP
Sbjct: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVNP 180

Query: 181 GDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRHE 240
           GDVVILP FGAAVDEMLVL DKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRHE
Sbjct: 181 GDVVILPAFGAAVDEMLVLKDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRHE 240

Query: 241 ETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDYD 300
           ETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDYD
Sbjct: 241 ETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDYD 300

Query: 301 LIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQ-ERQDAMY 360
           LIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQ +RQDAMY
Sbjct: 301 LIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQVKRQDAMY 360

Query: 361 QLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIHGELA 420
           QLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLI+GELA
Sbjct: 361 QLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIYGELA 420

Query: 421 EKENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQLA 465
           EKENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQLA
Sbjct: 421 EKENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQLA 465

[Close]

BLAST of MS020514.1 vs. ExPASy TrEMBL
Match: A0A6J1FWL7 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448851 PE=3 SV=1)

HSP 1 Score: 790.0 bits (2039), Expect = 5.3e-225
Identity = 389/463 (84.02%), Postives = 422/463 (91.14%), Query Frame = 0


Query: 1   MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60
           MAISLQIPR+  R   +S    SA GA  LR RKP  +RCTADS S    ++PDFDAKLF
Sbjct: 1   MAISLQIPRLHLRVDSVSSKRFSAGGA--LRCRKPLLVRCTADSSS----VNPDFDAKLF 60

Query: 61  WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120
           WKN++RGENYNRKGFGHKEET+KLM+SEFTSEV+NTLNENGN FTWGNVTVKLA AYGFC
Sbjct: 61  WKNFSRGENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFC 120

Query: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVNP 180
           +GVERAVQIA+EARKQFPS TIWITNEIIHNPTVNKRL EMGV+ IPLEE KK+FDVVNP
Sbjct: 121 WGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNP 180

Query: 181 GDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRHE 240
           GDVVILP FGAAVDEMLVL+DK V+IVDTTCPWVSKVWN VDKHKK EYTSIIHGKYRHE
Sbjct: 181 GDVVILPAFGAAVDEMLVLSDKKVKIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYRHE 240

Query: 241 ETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDYD 300
           ET+ATASFAGKYIIVK+M EATYV D+ILGVE NG I TKE FLKKF+SA+S+GFDPD D
Sbjct: 241 ETVATASFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEAFLKKFKSALSKGFDPDSD 300

Query: 301 LIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQERQDAMYQ 360
           L+KVGIANQTTMLKGETEEIGKLIE+TMMRKYG+EN+N+HF+SFNTIC+ATQ RQDAMYQ
Sbjct: 301 LMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFISFNTICNATQVRQDAMYQ 360

Query: 361 LVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIHGELAE 420
           LVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKL HGELA+
Sbjct: 361 LVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLNHGELAK 420

Query: 421 KENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQL 464
           KENWLPDGHVTIGVTSGASTPDKVV NVLKKVF++KGEGT Q+
Sbjct: 421 KENWLPDGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQV 457

[Close]

BLAST of MS020514.1 vs. ExPASy TrEMBL
Match: A0A1S3CAB0 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498435 PE=3 SV=1)

HSP 1 Score: 783.5 bits (2022), Expect = 4.9e-223
Identity = 382/463 (82.51%), Postives = 417/463 (90.06%), Query Frame = 0


Query: 1   MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60
           MAISLQ PR PHR  F  P   S  GA  LR R+P  LRCTADS SSP P+DPDF+AKLF
Sbjct: 1   MAISLQSPRFPHRIDFSLPKTFSGVGA--LRCRQPLLLRCTADSSSSPKPVDPDFNAKLF 60

Query: 61  WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120
            KN++RGENYNRKGFGHKEET+KLMNSEFTSE++ TL ENG +FTWGNVTVKLA AYGFC
Sbjct: 61  LKNFSRGENYNRKGFGHKEETVKLMNSEFTSEIVTTLKENGFQFTWGNVTVKLAGAYGFC 120

Query: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEE-RKKQFDVVN 180
           +GVERAVQ A+EARKQ+PS TIWITNEIIHNPTVNKRL EMGVH IPLEE  KK+FD VN
Sbjct: 121 WGVERAVQFAYEARKQYPSETIWITNEIIHNPTVNKRLEEMGVHNIPLEEGNKKRFDAVN 180

Query: 181 PGDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRH 240
           PGDVVILP FGAAVDE+LVLNDK V+IVDTTCPWVSKVWN V KHKK EYTSIIHGKY H
Sbjct: 181 PGDVVILPAFGAAVDEILVLNDKKVKIVDTTCPWVSKVWNAVGKHKKGEYTSIIHGKYSH 240

Query: 241 EETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDY 300
           EETIATASFAGKYIIVK+MAEA YVSD+ILGVE +GSI TKEEFLKKF+SAVS+GFDPD 
Sbjct: 241 EETIATASFAGKYIIVKDMAEAAYVSDYILGVEHDGSISTKEEFLKKFKSAVSKGFDPDI 300

Query: 301 DLIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQERQDAMY 360
            L+K+GIANQTTMLKGETEEIGKL+E+TMMRKYGVEN+NEHF+SFNTIC+ATQ RQDAMY
Sbjct: 301 HLVKIGIANQTTMLKGETEEIGKLMEKTMMRKYGVENINEHFISFNTICNATQVRQDAMY 360

Query: 361 QLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIHGELA 420
           +LV+ED+DLMLVVGGWNSSNTSHLQEIAEARG+PSYWIDSEQRIGPGNSISYKL HGELA
Sbjct: 361 ELVKEDVDLMLVVGGWNSSNTSHLQEIAEARGVPSYWIDSEQRIGPGNSISYKLAHGELA 420

Query: 421 EKENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQ 463
            KENWLPDGH+TIGVTSGASTPDKVV +VLKKVFD+KG+GT Q
Sbjct: 421 NKENWLPDGHITIGVTSGASTPDKVVGDVLKKVFDIKGDGTAQ 461

[Close]

BLAST of MS020514.1 vs. ExPASy TrEMBL
Match: A0A6J1JG99 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484238 PE=3 SV=1)

HSP 1 Score: 776.2 bits (2003), Expect = 7.8e-221
Identity = 382/463 (82.51%), Postives = 416/463 (89.85%), Query Frame = 0


Query: 1   MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGPLDPDFDAKLF 60
           MAISLQIPR+P R   +S    S  GA  LR  KP  +RCTADS S    ++PDFDAKLF
Sbjct: 1   MAISLQIPRLPLRVDSVSSKRFSGGGA--LRCWKPLLVRCTADSSS----VNPDFDAKLF 60

Query: 61  WKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGFC 120
           WKN++RGENYNRKGF HKEET+KLM+SEFTSEV+NTLNENGN FTWGNVTVKLA AYGFC
Sbjct: 61  WKNFSRGENYNRKGFEHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFC 120

Query: 121 YGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVNP 180
           +GVERAVQIA+EARKQFPS TIWITNEIIHNPTVNKRL EMGV+ IPLEE KK+FDVVNP
Sbjct: 121 WGVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNP 180

Query: 181 GDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKYRHE 240
           GDVVILP FGAAVDEMLVL+DK V IVDTTCPWVSKVWN VDKHKK EYTSIIHGKY HE
Sbjct: 181 GDVVILPAFGAAVDEMLVLSDKKVTIVDTTCPWVSKVWNAVDKHKKGEYTSIIHGKYHHE 240

Query: 241 ETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDPDYD 300
           ET+ATASFAGKYIIVK+M EATYV D+ILGVE NG I TKE+FLKKF+SAVS+GFDPD D
Sbjct: 241 ETVATASFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEDFLKKFKSAVSKGFDPDSD 300

Query: 301 LIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQERQDAMYQ 360
           L+KVGIANQTTMLK ET+EIGKLIE+TMMRKYG+EN++EHF+SFNTIC+ATQ RQDAMYQ
Sbjct: 301 LMKVGIANQTTMLKEETKEIGKLIEKTMMRKYGIENISEHFISFNTICNATQVRQDAMYQ 360

Query: 361 LVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIHGELAE 420
           LVEED DLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKL HGEL +
Sbjct: 361 LVEEDTDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLKHGELVK 420

Query: 421 KENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQL 464
           KENWLPDGH+TIGVTSGASTPD+VV NVLKKVF++KGEGT QL
Sbjct: 421 KENWLPDGHITIGVTSGASTPDQVVGNVLKKVFEIKGEGTAQL 457

[Close]

BLAST of MS020514.1 vs. ExPASy TrEMBL
Match: A0A2N9ECS7 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS380 PE=3 SV=1)

HSP 1 Score: 735.3 bits (1897), Expect = 1.5e-208
Identity = 361/466 (77.47%), Postives = 402/466 (86.27%), Query Frame = 0


Query: 1   MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPG---PLDPDFDA 60
           MAISLQ+ R+P R     P  L+  GA  LRLRKP S+RCT DS SS      +D DFDA
Sbjct: 1   MAISLQLCRIPTRTDLFLPESLAGPGA--LRLRKPLSVRCTGDSSSSSSSSVAVDSDFDA 60

Query: 61  KLFWKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAY 120
           K+F KN  R +NYNRKGFGHKEET++LMN E+TS++I TL ENGNE+TWGNVTVKLA AY
Sbjct: 61  KVFRKNLTRSKNYNRKGFGHKEETLELMNREYTSDIIKTLKENGNEYTWGNVTVKLAEAY 120

Query: 121 GFCYGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDV 180
           GFC+GVERAVQIA+EARKQFP   IWITNEIIHNPTVNKRL EM V  IP+EE +KQF+V
Sbjct: 121 GFCWGVERAVQIAYEARKQFPDEKIWITNEIIHNPTVNKRLEEMEVQNIPIEEGQKQFEV 180

Query: 181 VNPGDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGKY 240
           VN GDVV+LP FGAAVDEML L+DKNVQIVDTTCPWVSKVWN V+KHKK +YTSIIHGKY
Sbjct: 181 VNKGDVVVLPAFGAAVDEMLTLSDKNVQIVDTTCPWVSKVWNTVEKHKKGDYTSIIHGKY 240

Query: 241 RHEETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFDP 300
            HEET+ATASFAGKYIIVKNM EA YV D+ILG E NGS  TKEEFL+KF+ AVS+GFDP
Sbjct: 241 AHEETVATASFAGKYIIVKNMTEAMYVCDYILGGELNGSSSTKEEFLEKFKYAVSKGFDP 300

Query: 301 DYDLIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQERQDA 360
           D DL+K+GIANQTTMLKGETEEIGKL+ERTMMRKYGVENVNEHF+SFNTICDATQERQDA
Sbjct: 301 DSDLLKLGIANQTTMLKGETEEIGKLVERTMMRKYGVENVNEHFISFNTICDATQERQDA 360

Query: 361 MYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIHGE 420
           MY+LVEE +DLMLV+GGWNSSNTSHLQEIAE RGIPSYWIDSEQR+GPGN I+YKL HGE
Sbjct: 361 MYKLVEEKVDLMLVIGGWNSSNTSHLQEIAEDRGIPSYWIDSEQRVGPGNKIAYKLNHGE 420

Query: 421 LAEKENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQL 464
           L EKENWLP+G +TIGVTSGASTPDKVVE+VL KVFD+K E  +QL
Sbjct: 421 LVEKENWLPEGPITIGVTSGASTPDKVVEDVLIKVFDIKREEALQL 464

[Close]

BLAST of MS020514.1 vs. TAIR 10
Match: AT4G34350.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase )

HSP 1 Score: 685.3 bits (1767), Expect = 3.5e-197
Identity = 336/468 (71.79%), Postives = 394/468 (84.19%), Query Frame = 0


Query: 1   MAISLQIPRVPHRA-AFISPSPLSAAGALPLRLRKPFSLRCTADSPSSPGP---LDPDFD 60
           MA++LQ  R+  R   F+  + LS +G+  LR RK  S+RC++   ++P P   +D DFD
Sbjct: 1   MAVALQFSRLCVRPDTFVRENHLSGSGS--LRRRKALSVRCSSGDENAPSPSVVMDSDFD 60

Query: 61  AKLFWKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARA 120
           AK+F KN  R +NYNRKGFGHKEET+KLMN E+TS+++ TL  NG  ++WG+VTVKLA+A
Sbjct: 61  AKVFRKNLTRSDNYNRKGFGHKEETLKLMNREYTSDILETLKTNGYTYSWGDVTVKLAKA 120

Query: 121 YGFCYGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFD 180
           YGFC+GVERAVQIA+EARKQFP   +WITNEIIHNPTVNKRL +M V  IP+E+ KKQFD
Sbjct: 121 YGFCWGVERAVQIAYEARKQFPEERLWITNEIIHNPTVNKRLEDMDVKIIPVEDSKKQFD 180

Query: 181 VVNPGDVVILPTFGAAVDEMLVLNDKNVQIVDTTCPWVSKVWNVVDKHKKREYTSIIHGK 240
           VV   DVVILP FGA VDEM VLNDK VQIVDTTCPWV+KVWN V+KHKK EYTS+IHGK
Sbjct: 181 VVEKDDVVILPAFGAGVDEMYVLNDKKVQIVDTTCPWVTKVWNTVEKHKKGEYTSVIHGK 240

Query: 241 YRHEETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTKEEFLKKFESAVSRGFD 300
           Y HEETIATASFAGKYIIVKNM EA YV D+ILG + +GS  TKEEF++KF+ A+S+GFD
Sbjct: 241 YNHEETIATASFAGKYIIVKNMKEANYVCDYILGGQYDGSSSTKEEFMEKFKYAISKGFD 300

Query: 301 PDYDLIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEHFVSFNTICDATQERQD 360
           PD DL+KVGIANQTTMLKGETEEIG+L+E TMMRKYGVENV+ HF+SFNTICDATQERQD
Sbjct: 301 PDNDLVKVGIANQTTMLKGETEEIGRLLETTMMRKYGVENVSGHFISFNTICDATQERQD 360

Query: 361 AMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQRIGPGNSISYKLIHG 420
           A+Y+LVEE +DLMLVVGGWNSSNTSHLQEI+EARGIPSYWIDSE+RIGPGN I+YKL +G
Sbjct: 361 AIYELVEEKIDLMLVVGGWNSSNTSHLQEISEARGIPSYWIDSEKRIGPGNKIAYKLHYG 420

Query: 421 ELAEKENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEGTVQLA 465
           EL EKEN+LP G +TIGVTSGASTPDKVVE+ L KVFD+K E  +QLA
Sbjct: 421 ELVEKENFLPKGPITIGVTSGASTPDKVVEDALVKVFDIKREELLQLA 466

The following BLAST results are available for this feature:

BLAST of MS020514.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148542.1	1.8e-267	98.92	4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like [Momordic...	[more]
XP_038902065.1	5.2e-227	84.02	4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X...	[more]
XP_004145400.1	2.6e-226	83.37	4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Cucumis sativ...	[more]
XP_023512039.1	4.4e-226	84.23	4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X...	[more]
XP_022944389.1	1.1e-224	84.02	4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X...	[more]

BLAST of MS020514.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94B35	4.9e-196	71.79	4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Arabidopsis...	[more]
Q6AVG6	1.4e-190	71.87	4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Oryza sativ...	[more]
B1WTZ2	4.7e-146	61.33	4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Crocosphaera subtropica (s...	[more]
B8HWD3	6.3e-143	61.19	4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cyanothece sp. (strain PCC...	[more]
B2IZV5	2.4e-142	60.60	4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Nostoc punctiforme (strain...	[more]

BLAST of MS020514.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D5C2	8.6e-268	98.92	4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like OS=Momord...	[more]
A0A6J1FWL7	5.3e-225	84.02	4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X...	[more]
A0A1S3CAB0	4.9e-223	82.51	4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X...	[more]
A0A6J1JG99	7.8e-221	82.51	4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X...	[more]
A0A2N9ECS7	1.5e-208	77.47	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS380 PE=3 SV=1	[more]

BLAST of MS020514.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G34350.1	3.5e-197	71.79	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.1010.20		coord: 332..463 e-value: 1.1E-40 score: 139.8
None	No IPR available	GENE3D	3.40.1010.20		coord: 212..331 e-value: 3.7E-12 score: 47.9
None	No IPR available	GENE3D	3.40.50.11270		coord: 120..210 e-value: 1.7E-20 score: 74.8
None	No IPR available	PANTHER	PTHR31619	4-HYDROXY-3-METHYLBUT-2-ENYL DIPHOSPHATE REDUCTASE, CHLOROPLASTIC	coord: 31..460
IPR003451	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	TIGRFAM	TIGR00216	TIGR00216	coord: 111..452 e-value: 9.3E-91 score: 301.8
IPR003451	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	PFAM	PF02401	LYTB	coord: 111..452 e-value: 1.4E-84 score: 283.5
IPR003451	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	HAMAP	MF_00191	IspH	coord: 109..456 score: 28.719051
IPR003451	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	CDD	cd13944	lytB_ispH	coord: 111..452 e-value: 7.17632E-109 score: 321.667

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS020514	MS020514	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS020514.1-cds	MS020514.1-cds-scaffold375:797107..797169	CDS
MS020514.1-cds	MS020514.1-cds-scaffold375:797283..797369	CDS
MS020514.1-cds	MS020514.1-cds-scaffold375:797462..797647	CDS
MS020514.1-cds	MS020514.1-cds-scaffold375:798160..798254	CDS
MS020514.1-cds	MS020514.1-cds-scaffold375:798350..798455	CDS
MS020514.1-cds	MS020514.1-cds-scaffold375:798549..798623	CDS
MS020514.1-cds	MS020514.1-cds-scaffold375:798722..798853	CDS
MS020514.1-cds	MS020514.1-cds-scaffold375:799020..799199	CDS
MS020514.1-cds	MS020514.1-cds-scaffold375:799504..799700	CDS
MS020514.1-cds	MS020514.1-cds-scaffold375:799772..800042	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS020514.1	MS020514.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050992	dimethylallyl diphosphate biosynthetic process
biological_process	GO:0019288	isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
molecular_function	GO:0051745	4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity
molecular_function	GO:0046872	metal ion binding